Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026780.t1 | XP_013454666.1 | 96 | 175 | 5 | 1 | 1 | 173 | 1 | 175 | 4.10E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026780.t1 | Q8CXK5 | 28.1 | 128 | 75 | 4 | 9 | 131 | 1 | 116 | 2.7e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026780.t1 | A0A072UG80 | 96.0 | 175 | 5 | 1 | 1 | 173 | 1 | 175 | 3.0e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60227 | MS.gene026780 | PPI |
| MS.gene75379 | MS.gene026780 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026780.t1 | MTR_4g011020 | 98.857 | 175 | 0 | 1 | 1 | 173 | 1 | 175 | 2.78e-126 | 352 |
| MS.gene026780.t1 | MTR_2g103490 | 74.713 | 174 | 38 | 2 | 1 | 173 | 1 | 169 | 1.62e-90 | 261 |
| MS.gene026780.t1 | MTR_2g103460 | 71.084 | 166 | 47 | 1 | 1 | 165 | 1 | 166 | 7.45e-83 | 242 |
| MS.gene026780.t1 | MTR_4g114080 | 67.105 | 152 | 49 | 1 | 5 | 155 | 1 | 152 | 7.26e-77 | 227 |
| MS.gene026780.t1 | MTR_1g115170 | 68.531 | 143 | 44 | 1 | 24 | 165 | 1 | 143 | 2.97e-66 | 199 |
| MS.gene026780.t1 | MTR_2g005880 | 62.500 | 136 | 51 | 0 | 8 | 143 | 2 | 137 | 2.35e-61 | 188 |
| MS.gene026780.t1 | MTR_5g021610 | 54.362 | 149 | 60 | 2 | 6 | 148 | 22 | 168 | 8.95e-56 | 175 |
| MS.gene026780.t1 | MTR_8g076160 | 54.286 | 140 | 60 | 1 | 8 | 143 | 12 | 151 | 1.15e-54 | 171 |
| MS.gene026780.t1 | MTR_0003s0630 | 47.977 | 173 | 82 | 4 | 2 | 168 | 29 | 199 | 1.28e-53 | 169 |
| MS.gene026780.t1 | MTR_4g125860 | 29.365 | 126 | 82 | 2 | 13 | 136 | 72 | 192 | 5.09e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026780.t1 | AT1G80160 | 79.290 | 169 | 32 | 1 | 1 | 169 | 1 | 166 | 4.26e-97 | 278 |
| MS.gene026780.t1 | AT1G80160 | 79.290 | 169 | 32 | 1 | 1 | 169 | 42 | 207 | 8.22e-97 | 279 |
| MS.gene026780.t1 | AT1G15380 | 75.000 | 172 | 43 | 0 | 1 | 172 | 1 | 172 | 8.30e-97 | 278 |
| MS.gene026780.t1 | AT1G15380 | 75.000 | 172 | 43 | 0 | 1 | 172 | 1 | 172 | 8.30e-97 | 278 |
| MS.gene026780.t1 | AT1G80160 | 77.778 | 117 | 23 | 1 | 53 | 169 | 27 | 140 | 2.47e-60 | 184 |
| MS.gene026780.t1 | AT2G28420 | 54.478 | 134 | 59 | 2 | 11 | 142 | 18 | 151 | 1.45e-53 | 168 |
Find 35 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAACCAGGCCTTCTTAT+TGG | 0.200598 | 4.1:+85118604 | None:intergenic |
| TTCTTCCTTGGTAACGTCTC+TGG | 0.223323 | 4.1:+85118217 | None:intergenic |
| GACCTAATTTGCAGGCTATT+TGG | 0.231443 | 4.1:-85118277 | MS.gene026780:intron |
| GATTTCTACCAGAATGTTCT+TGG | 0.277645 | 4.1:-85118634 | MS.gene026780:CDS |
| GGATTTATTTCTTTCTTCCT+TGG | 0.309354 | 4.1:+85118205 | None:intergenic |
| AAGAACCAGGCCTTCTTATT+GGG | 0.313845 | 4.1:+85118605 | None:intergenic |
| AGGCTATTTGGCTATGGTAT+TGG | 0.365518 | 4.1:-85118265 | MS.gene026780:CDS |
| ATCTCAATCATGAAACCATC+TGG | 0.372159 | 4.1:+85117259 | None:intergenic |
| CTTGCTACCTCACCAACTAA+TGG | 0.386113 | 4.1:+85117208 | None:intergenic |
| AAAGATATGTGATTATCCTT+TGG | 0.393062 | 4.1:+85118184 | None:intergenic |
| GGAAGAAACCAAGAACATTC+TGG | 0.404879 | 4.1:+85118626 | None:intergenic |
| GGATAATCACATATCTTTCC+AGG | 0.407468 | 4.1:-85118179 | MS.gene026780:intron |
| CTTCCTGTGATTCCATTAGT+TGG | 0.419159 | 4.1:-85117220 | MS.gene026780:CDS |
| TCTTCCTTGGTAACGTCTCT+GGG | 0.440829 | 4.1:+85118218 | None:intergenic |
| TTGGTTTCTTCCCAATAAGA+AGG | 0.469291 | 4.1:-85118615 | MS.gene026780:CDS |
| ATTTGCAGGCTATTTGGCTA+TGG | 0.473328 | 4.1:-85118271 | MS.gene026780:intron |
| TTCTTCCCAATAAGAAGGCC+TGG | 0.475428 | 4.1:-85118610 | MS.gene026780:CDS |
| TGATGTGCAGTGTGAGAGCA+TGG | 0.503338 | 4.1:-85117380 | MS.gene026780:intron |
| TCATGAAACCATCTGGGTCA+TGG | 0.503506 | 4.1:+85117266 | None:intergenic |
| GATGTGCAGTGTGAGAGCAT+GGG | 0.508852 | 4.1:-85117379 | MS.gene026780:intron |
| TGAGAAAATGAAGGAAAGTG+TGG | 0.520447 | 4.1:-85118725 | None:intergenic |
| AGAGGAAGGTGGGATTGAAG+TGG | 0.523433 | 4.1:-85117302 | MS.gene026780:CDS |
| TCACCAACTAATGGAATCAC+AGG | 0.547180 | 4.1:+85117217 | None:intergenic |
| TGTGATTCCATTAGTTGGTG+AGG | 0.549148 | 4.1:-85117215 | MS.gene026780:CDS |
| GAGCAAGAGTAGAGGAAGGT+GGG | 0.575892 | 4.1:-85117312 | MS.gene026780:CDS |
| GAGAAAATGAAGGAAAGTGT+GGG | 0.578884 | 4.1:-85118724 | None:intergenic |
| CGAGCAAGAGTAGAGGAAGG+TGG | 0.581051 | 4.1:-85117313 | MS.gene026780:CDS |
| GAAGAAAGAAATAAATCCAA+AGG | 0.593095 | 4.1:-85118200 | MS.gene026780:CDS |
| CCATCAAAATCAAAAGAACC+AGG | 0.595725 | 4.1:+85118592 | None:intergenic |
| ATGTGCAGTGTGAGAGCATG+GGG | 0.599437 | 4.1:-85117378 | MS.gene026780:intron |
| TCTCAATCATGAAACCATCT+GGG | 0.612866 | 4.1:+85117260 | None:intergenic |
| TTATGTTCGAGCAAGAGTAG+AGG | 0.637893 | 4.1:-85117320 | MS.gene026780:CDS |
| AGAACCCAGAGACGTTACCA+AGG | 0.641794 | 4.1:-85118222 | MS.gene026780:CDS |
| GTTCGAGCAAGAGTAGAGGA+AGG | 0.649580 | 4.1:-85117316 | MS.gene026780:CDS |
| TGTGCAGTGTGAGAGCATGG+GGG | 0.820385 | 4.1:-85117377 | MS.gene026780:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTATATTTAGTTTTAAATT+TGG | + | chr4.1:85117851-85117870 | None:intergenic | 10.0% |
| !! | ACACAAAATTTCATATTAAA+TGG | + | chr4.1:85118433-85118452 | None:intergenic | 15.0% |
| !! | TTTGTTAAAACAACTATTAT+GGG | - | chr4.1:85117365-85117384 | MS.gene026780:CDS | 15.0% |
| !!! | AGAAAAAATACTAAATTTTG+AGG | + | chr4.1:85117493-85117512 | None:intergenic | 15.0% |
| !!! | GAAAAAATACTAAATTTTGA+GGG | + | chr4.1:85117492-85117511 | None:intergenic | 15.0% |
| !!! | TTTTGTTAAAACAACTATTA+TGG | - | chr4.1:85117364-85117383 | MS.gene026780:CDS | 15.0% |
| !! | AAATTTAATAAACCTTTGCT+TGG | - | chr4.1:85117517-85117536 | MS.gene026780:intron | 20.0% |
| !! | ATCCAACTTTCTTAATTTAA+TGG | - | chr4.1:85118210-85118229 | MS.gene026780:CDS | 20.0% |
| !! | ATTGATTACGTTTCAAAATT+TGG | - | chr4.1:85117720-85117739 | MS.gene026780:intron | 20.0% |
| !! | TACCATTAAATTAAGAAAGT+TGG | + | chr4.1:85118215-85118234 | None:intergenic | 20.0% |
| !!! | ATTTTGAAACGTAATCAATA+AGG | + | chr4.1:85117719-85117738 | None:intergenic | 20.0% |
| !!! | GAGTGTTTTAGTTTTAAATT+TGG | + | chr4.1:85117879-85117898 | None:intergenic | 20.0% |
| !!! | TGAAAATTGATTTTTGTATG+TGG | - | chr4.1:85117549-85117568 | MS.gene026780:intron | 20.0% |
| ! | AAAGATATGTGATTATCCTT+TGG | + | chr4.1:85117669-85117688 | None:intergenic | 25.0% |
| ! | GAAGAAAGAAATAAATCCAA+AGG | - | chr4.1:85117650-85117669 | MS.gene026780:intron | 25.0% |
| ! | GTGCAAAAAATTACATGATT+CGG | + | chr4.1:85118009-85118028 | None:intergenic | 25.0% |
| ! | GTTTCATCAGAAATTAAACT+TGG | - | chr4.1:85118368-85118387 | MS.gene026780:intron | 25.0% |
| ! | TTTGTGTTTGAACTTTGAAT+TGG | - | chr4.1:85118446-85118465 | MS.gene026780:intron | 25.0% |
| !! | CGTTTATGTTATCCTTTTTA+AGG | - | chr4.1:85117917-85117936 | MS.gene026780:intron | 25.0% |
| !! | CTCAACAATTTGTCATTTAT+CGG | - | chr4.1:85118397-85118416 | MS.gene026780:intron | 25.0% |
| !!! | AGATATGATTCACTGATTTT+AGG | + | chr4.1:85117164-85117183 | None:intergenic | 25.0% |
| ACAATGCATAATATATGCAG+AGG | - | chr4.1:85118103-85118122 | MS.gene026780:intron | 30.0% | |
| AGACGTACTTCAATAAATGA+AGG | - | chr4.1:85117778-85117797 | MS.gene026780:intron | 30.0% | |
| CGATAAATGACAAATTGTTG+AGG | + | chr4.1:85118399-85118418 | None:intergenic | 30.0% | |
| TCATTTAGAAAACCAAGCAA+AGG | + | chr4.1:85117532-85117551 | None:intergenic | 30.0% | |
| TTTAAGGATCTTATCCTCTA+AGG | - | chr4.1:85117955-85117974 | MS.gene026780:intron | 30.0% | |
| ! | GGATTTATTTCTTTCTTCCT+TGG | + | chr4.1:85117648-85117667 | None:intergenic | 30.0% |
| !! | TCCTTTTTAAGGATATGCAA+TGG | - | chr4.1:85117928-85117947 | MS.gene026780:intron | 30.0% |
| !!! | ATGTAATTTTTTGCACTGCA+CGG | - | chr4.1:85118013-85118032 | MS.gene026780:intron | 30.0% |
| !!! | GATATGCAATGGCTTTTTTA+AGG | - | chr4.1:85117939-85117958 | MS.gene026780:intron | 30.0% |
| !!! | TTTTTCTATGCGTCACTTTT+AGG | + | chr4.1:85117827-85117846 | None:intergenic | 30.0% |
| !!! | TTTTTTATCAAGGAGACTAG+TGG | + | chr4.1:85118178-85118197 | None:intergenic | 30.0% |
| ATCAGACTATACTTACCTCA+CGG | + | chr4.1:85118340-85118359 | None:intergenic | 35.0% | |
| ATCTCAATCATGAAACCATC+TGG | + | chr4.1:85118594-85118613 | None:intergenic | 35.0% | |
| CCATCAAAATCAAAAGAACC+AGG | + | chr4.1:85117261-85117280 | None:intergenic | 35.0% | |
| GATTTCTACCAGAATGTTCT+TGG | - | chr4.1:85117216-85117235 | MS.gene026780:CDS | 35.0% | |
| GCCATTGCATATCCTTAAAA+AGG | + | chr4.1:85117932-85117951 | None:intergenic | 35.0% | |
| GGAGAAAAAGCTGAAAGAAA+TGG | - | chr4.1:85118500-85118519 | MS.gene026780:intron | 35.0% | |
| GGATAATCACATATCTTTCC+AGG | - | chr4.1:85117671-85117690 | MS.gene026780:intron | 35.0% | |
| GTTTAGTGAAAAATCTGACC+TGG | + | chr4.1:85117692-85117711 | None:intergenic | 35.0% | |
| TAAACGTGTCTATGATGACT+AGG | + | chr4.1:85117903-85117922 | None:intergenic | 35.0% | |
| TAGTCTATGTGAGCTTAACT+CGG | - | chr4.1:85118071-85118090 | MS.gene026780:intron | 35.0% | |
| TCTCAATCATGAAACCATCT+GGG | + | chr4.1:85118593-85118612 | None:intergenic | 35.0% | |
| TGCATAATATATGCAGAGGT+CGG | - | chr4.1:85118107-85118126 | MS.gene026780:intron | 35.0% | |
| TGTTAACCATTCGATTCTCA+GGG | - | chr4.1:85118286-85118305 | MS.gene026780:intron | 35.0% | |
| TTGGTTTCTTCCCAATAAGA+AGG | - | chr4.1:85117235-85117254 | MS.gene026780:CDS | 35.0% | |
| TTGTTAACCATTCGATTCTC+AGG | - | chr4.1:85118285-85118304 | MS.gene026780:intron | 35.0% | |
| !!! | CCTGGTTCTTTTGATTTTGA+TGG | - | chr4.1:85117258-85117277 | MS.gene026780:CDS | 35.0% |
| !!! | CTTTTGATTTTGATGGAGCA+TGG | - | chr4.1:85117265-85117284 | MS.gene026780:CDS | 35.0% |
| !!! | TCACTGATTTTAGGTGCAAA+GGG | + | chr4.1:85117155-85117174 | None:intergenic | 35.0% |
| !!! | TTCACTGATTTTAGGTGCAA+AGG | + | chr4.1:85117156-85117175 | None:intergenic | 35.0% |
| AAAGAACCAGGCCTTCTTAT+TGG | + | chr4.1:85117249-85117268 | None:intergenic | 40.0% | |
| AAGAACCAGGCCTTCTTATT+GGG | + | chr4.1:85117248-85117267 | None:intergenic | 40.0% | |
| AGCCAAATAGCCTGCAAATT+AGG | + | chr4.1:85117578-85117597 | None:intergenic | 40.0% | |
| AGGCTATTTGGCTATGGTAT+TGG | - | chr4.1:85117585-85117604 | MS.gene026780:intron | 40.0% | |
| ATTTGCAGGCTATTTGGCTA+TGG | - | chr4.1:85117579-85117598 | MS.gene026780:intron | 40.0% | |
| CATAATATATGCAGAGGTCG+GGG | - | chr4.1:85118109-85118128 | MS.gene026780:intron | 40.0% | |
| CTTCCTGTGATTCCATTAGT+TGG | - | chr4.1:85118630-85118649 | MS.gene026780:CDS | 40.0% | |
| GCATAATATATGCAGAGGTC+GGG | - | chr4.1:85118108-85118127 | MS.gene026780:intron | 40.0% | |
| GCATCGTGCAATTGTAGTAA+AGG | - | chr4.1:85118237-85118256 | MS.gene026780:CDS | 40.0% | |
| GGAAGAAACCAAGAACATTC+TGG | + | chr4.1:85117227-85117246 | None:intergenic | 40.0% | |
| TCACCAACTAATGGAATCAC+AGG | + | chr4.1:85118636-85118655 | None:intergenic | 40.0% | |
| TTATGTTCGAGCAAGAGTAG+AGG | - | chr4.1:85118530-85118549 | MS.gene026780:intron | 40.0% | |
| ! | GACCTAATTTGCAGGCTATT+TGG | - | chr4.1:85117573-85117592 | MS.gene026780:intron | 40.0% |
| ! | GCAATTTGAAGCAACCTTAG+AGG | + | chr4.1:85117972-85117991 | None:intergenic | 40.0% |
| ! | GCTCACACATTTTAAACACG+AGG | + | chr4.1:85118150-85118169 | None:intergenic | 40.0% |
| ! | GGAAAAGAGCTCTGGTAATT+CGG | - | chr4.1:85118307-85118326 | MS.gene026780:intron | 40.0% |
| ! | TATGTGGTGACCTAATTTGC+AGG | - | chr4.1:85117565-85117584 | MS.gene026780:intron | 40.0% |
| ! | TCTTTTCCCTGAGAATCGAA+TGG | + | chr4.1:85118295-85118314 | None:intergenic | 40.0% |
| ! | TGTGATTCCATTAGTTGGTG+AGG | - | chr4.1:85118635-85118654 | MS.gene026780:CDS | 40.0% |
| !! | CATTTTAAACACGAGGTGTC+TGG | + | chr4.1:85118143-85118162 | None:intergenic | 40.0% |
| !! | CTATTTTTCCATGACCCAGA+TGG | - | chr4.1:85118576-85118595 | MS.gene026780:intron | 40.0% |
| ATTCTCAGGGAAAAGAGCTC+TGG | - | chr4.1:85118299-85118318 | MS.gene026780:intron | 45.0% | |
| CTATGTGAGCTTAACTCGGT+TGG | - | chr4.1:85118075-85118094 | MS.gene026780:intron | 45.0% | |
| CTTGCTACCTCACCAACTAA+TGG | + | chr4.1:85118645-85118664 | None:intergenic | 45.0% | |
| TCATGAAACCATCTGGGTCA+TGG | + | chr4.1:85118587-85118606 | None:intergenic | 45.0% | |
| TTCTTCCCAATAAGAAGGCC+TGG | - | chr4.1:85117240-85117259 | MS.gene026780:CDS | 45.0% | |
| ! | TGAGCTTAACTCGGTTGGTA+GGG | - | chr4.1:85118080-85118099 | MS.gene026780:intron | 45.0% |
| !! | TCTTCCTTGGTAACGTCTCT+GGG | + | chr4.1:85117635-85117654 | None:intergenic | 45.0% |
| !! | TTCTTCCTTGGTAACGTCTC+TGG | + | chr4.1:85117636-85117655 | None:intergenic | 45.0% |
| !!! | TAATATTTTTTTTTTTATCA+AGG | + | chr4.1:85118188-85118207 | None:intergenic | 5.0% |
| AGAACCCAGAGACGTTACCA+AGG | - | chr4.1:85117628-85117647 | MS.gene026780:intron | 50.0% | |
| AGAGGAAGGTGGGATTGAAG+TGG | - | chr4.1:85118548-85118567 | MS.gene026780:intron | 50.0% | |
| GAGCAAGAGTAGAGGAAGGT+GGG | - | chr4.1:85118538-85118557 | MS.gene026780:intron | 50.0% | |
| GTGAGCTTAACTCGGTTGGT+AGG | - | chr4.1:85118079-85118098 | MS.gene026780:intron | 50.0% | |
| GTTCGAGCAAGAGTAGAGGA+AGG | - | chr4.1:85118534-85118553 | MS.gene026780:intron | 50.0% | |
| TAATTCGGAGTTCGACCGTG+AGG | - | chr4.1:85118322-85118341 | MS.gene026780:intron | 50.0% | |
| TGATGTGCAGTGTGAGAGCA+TGG | - | chr4.1:85118470-85118489 | MS.gene026780:intron | 50.0% | |
| ! | ATGTGCAGTGTGAGAGCATG+GGG | - | chr4.1:85118472-85118491 | MS.gene026780:intron | 50.0% |
| ! | GATGTGCAGTGTGAGAGCAT+GGG | - | chr4.1:85118471-85118490 | MS.gene026780:intron | 50.0% |
| CGAGCAAGAGTAGAGGAAGG+TGG | - | chr4.1:85118537-85118556 | MS.gene026780:intron | 55.0% | |
| ! | TGTGCAGTGTGAGAGCATGG+GGG | - | chr4.1:85118473-85118492 | MS.gene026780:intron | 55.0% |
| ! | GTGTGAGAGCATGGGGGCAG+TGG | - | chr4.1:85118479-85118498 | MS.gene026780:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 85117132 | 85118740 | 85117132 | ID=MS.gene026780 |
| chr4.1 | mRNA | 85117132 | 85118740 | 85117132 | ID=MS.gene026780.t1;Parent=MS.gene026780 |
| chr4.1 | exon | 85118586 | 85118740 | 85118586 | ID=MS.gene026780.t1.exon1;Parent=MS.gene026780.t1 |
| chr4.1 | CDS | 85118586 | 85118740 | 85118586 | ID=cds.MS.gene026780.t1;Parent=MS.gene026780.t1 |
| chr4.1 | exon | 85118180 | 85118285 | 85118180 | ID=MS.gene026780.t1.exon2;Parent=MS.gene026780.t1 |
| chr4.1 | CDS | 85118180 | 85118285 | 85118180 | ID=cds.MS.gene026780.t1;Parent=MS.gene026780.t1 |
| chr4.1 | exon | 85117132 | 85117392 | 85117132 | ID=MS.gene026780.t1.exon3;Parent=MS.gene026780.t1 |
| chr4.1 | CDS | 85117132 | 85117392 | 85117132 | ID=cds.MS.gene026780.t1;Parent=MS.gene026780.t1 |
| Gene Sequence |
| Protein sequence |
