Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60227.t1 | XP_013454666.1 | 96 | 175 | 5 | 1 | 1 | 173 | 1 | 175 | 4.10E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60227.t1 | Q8CXK5 | 28.1 | 128 | 75 | 4 | 9 | 131 | 1 | 116 | 2.7e-06 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60227.t1 | A0A072UG80 | 96.0 | 175 | 5 | 1 | 1 | 173 | 1 | 175 | 3.0e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052937 | MS.gene60227 | 0.805818 | 1.11E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75379 | MS.gene60227 | PPI |
| MS.gene60227 | MS.gene026780 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60227.t1 | MTR_4g011020 | 98.857 | 175 | 0 | 1 | 1 | 173 | 1 | 175 | 2.78e-126 | 352 |
| MS.gene60227.t1 | MTR_2g103490 | 74.713 | 174 | 38 | 2 | 1 | 173 | 1 | 169 | 1.62e-90 | 261 |
| MS.gene60227.t1 | MTR_2g103460 | 71.084 | 166 | 47 | 1 | 1 | 165 | 1 | 166 | 7.45e-83 | 242 |
| MS.gene60227.t1 | MTR_4g114080 | 67.105 | 152 | 49 | 1 | 5 | 155 | 1 | 152 | 7.26e-77 | 227 |
| MS.gene60227.t1 | MTR_1g115170 | 68.531 | 143 | 44 | 1 | 24 | 165 | 1 | 143 | 2.97e-66 | 199 |
| MS.gene60227.t1 | MTR_2g005880 | 62.500 | 136 | 51 | 0 | 8 | 143 | 2 | 137 | 2.35e-61 | 188 |
| MS.gene60227.t1 | MTR_5g021610 | 54.362 | 149 | 60 | 2 | 6 | 148 | 22 | 168 | 8.95e-56 | 175 |
| MS.gene60227.t1 | MTR_8g076160 | 54.286 | 140 | 60 | 1 | 8 | 143 | 12 | 151 | 1.15e-54 | 171 |
| MS.gene60227.t1 | MTR_0003s0630 | 47.977 | 173 | 82 | 4 | 2 | 168 | 29 | 199 | 1.28e-53 | 169 |
| MS.gene60227.t1 | MTR_4g125860 | 29.365 | 126 | 82 | 2 | 13 | 136 | 72 | 192 | 5.09e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60227.t1 | AT1G80160 | 79.290 | 169 | 32 | 1 | 1 | 169 | 1 | 166 | 4.26e-97 | 278 |
| MS.gene60227.t1 | AT1G80160 | 79.290 | 169 | 32 | 1 | 1 | 169 | 42 | 207 | 8.22e-97 | 279 |
| MS.gene60227.t1 | AT1G15380 | 75.000 | 172 | 43 | 0 | 1 | 172 | 1 | 172 | 8.30e-97 | 278 |
| MS.gene60227.t1 | AT1G15380 | 75.000 | 172 | 43 | 0 | 1 | 172 | 1 | 172 | 8.30e-97 | 278 |
| MS.gene60227.t1 | AT1G80160 | 77.778 | 117 | 23 | 1 | 53 | 169 | 27 | 140 | 2.47e-60 | 184 |
| MS.gene60227.t1 | AT2G28420 | 54.478 | 134 | 59 | 2 | 11 | 142 | 18 | 151 | 1.45e-53 | 168 |
Find 35 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAACCAGGCCTTCTTAT+TGG | 0.200598 | 4.3:+85802565 | None:intergenic |
| TTCTTCCTTGGTAACGTCTC+TGG | 0.223323 | 4.3:+85802171 | None:intergenic |
| GACCTAATTTGCAGGCTATT+TGG | 0.231443 | 4.3:-85802231 | MS.gene60227:intron |
| GATTTCTACCAGAATGTTCT+TGG | 0.277645 | 4.3:-85802595 | MS.gene60227:CDS |
| GGATTTATTTCTTTCTTCCT+TGG | 0.309354 | 4.3:+85802159 | None:intergenic |
| AAGAACCAGGCCTTCTTATT+GGG | 0.313845 | 4.3:+85802566 | None:intergenic |
| AGGCTATTTGGCTATGGTAT+TGG | 0.365518 | 4.3:-85802219 | MS.gene60227:CDS |
| ATCTCAATCATGAAACCATC+TGG | 0.372159 | 4.3:+85801204 | None:intergenic |
| CTTGCTACCTCACCAACTAA+TGG | 0.386113 | 4.3:+85801153 | None:intergenic |
| AAAGATATGTGATTATCCTT+TGG | 0.393062 | 4.3:+85802138 | None:intergenic |
| GGAAGAAACCAAGAACATTC+TGG | 0.404879 | 4.3:+85802587 | None:intergenic |
| GGATAATCACATATCTTTCC+AGG | 0.407468 | 4.3:-85802133 | MS.gene60227:intron |
| CTTCCTGTGATTCCATTAGT+TGG | 0.419159 | 4.3:-85801165 | MS.gene60227:CDS |
| TCTTCCTTGGTAACGTCTCT+GGG | 0.440829 | 4.3:+85802172 | None:intergenic |
| TTGGTTTCTTCCCAATAAGA+AGG | 0.469291 | 4.3:-85802576 | MS.gene60227:CDS |
| ATTTGCAGGCTATTTGGCTA+TGG | 0.473328 | 4.3:-85802225 | MS.gene60227:intron |
| TTCTTCCCAATAAGAAGGCC+TGG | 0.475428 | 4.3:-85802571 | MS.gene60227:CDS |
| TGATGTGCAGTGTGAGAGCA+TGG | 0.503338 | 4.3:-85801325 | MS.gene60227:intron |
| TCATGAAACCATCTGGGTCA+TGG | 0.503506 | 4.3:+85801211 | None:intergenic |
| GATGTGCAGTGTGAGAGCAT+GGG | 0.508852 | 4.3:-85801324 | MS.gene60227:intron |
| TGAGAAAATGAAGGAAAGTG+TGG | 0.520447 | 4.3:-85802686 | None:intergenic |
| AGAGGAAGGTGGGATTGAAG+TGG | 0.523433 | 4.3:-85801247 | MS.gene60227:CDS |
| TCACCAACTAATGGAATCAC+AGG | 0.547180 | 4.3:+85801162 | None:intergenic |
| TGTGATTCCATTAGTTGGTG+AGG | 0.549148 | 4.3:-85801160 | MS.gene60227:CDS |
| GAGAAAATGAAGGAAAGTGT+GGG | 0.559061 | 4.3:-85802685 | None:intergenic |
| GAGCAAGAGTAGAGGAAGGT+GGG | 0.575892 | 4.3:-85801257 | MS.gene60227:CDS |
| CGAGCAAGAGTAGAGGAAGG+TGG | 0.581051 | 4.3:-85801258 | MS.gene60227:CDS |
| GAAGAAAGAAATAAATCCAA+AGG | 0.593095 | 4.3:-85802154 | MS.gene60227:CDS |
| CCATCAAAATCAAAAGAACC+AGG | 0.595725 | 4.3:+85802553 | None:intergenic |
| ATGTGCAGTGTGAGAGCATG+GGG | 0.599437 | 4.3:-85801323 | MS.gene60227:intron |
| TCTCAATCATGAAACCATCT+GGG | 0.612866 | 4.3:+85801205 | None:intergenic |
| TTATGTTCGAGCAAGAGTAG+AGG | 0.637893 | 4.3:-85801265 | MS.gene60227:CDS |
| AGAACCCAGAGACGTTACCA+AGG | 0.641794 | 4.3:-85802176 | MS.gene60227:CDS |
| GTTCGAGCAAGAGTAGAGGA+AGG | 0.649580 | 4.3:-85801261 | MS.gene60227:CDS |
| TGTGCAGTGTGAGAGCATGG+GGG | 0.820385 | 4.3:-85801322 | MS.gene60227:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAAATTTTAATAAATTTT+TGG | - | chr4.3:85801465-85801484 | MS.gene60227:intron | 0.0% |
| !!! | AATTTTAATAAATTTTTGGT+TGG | - | chr4.3:85801469-85801488 | MS.gene60227:intron | 10.0% |
| !!! | TCTATATTTAGTTTTAAATT+TGG | + | chr4.3:85801803-85801822 | None:intergenic | 10.0% |
| !! | ACACAAAATTTCATATTAAA+TGG | + | chr4.3:85802394-85802413 | None:intergenic | 15.0% |
| !! | TTTGTTAAAACAACTATTAT+GGG | - | chr4.3:85801317-85801336 | MS.gene60227:CDS | 15.0% |
| !!! | AGAAAAAATACTAAATTTTG+AGG | + | chr4.3:85801445-85801464 | None:intergenic | 15.0% |
| !!! | GAAAAAATACTAAATTTTGA+GGG | + | chr4.3:85801444-85801463 | None:intergenic | 15.0% |
| !!! | TTTTGTTAAAACAACTATTA+TGG | - | chr4.3:85801316-85801335 | MS.gene60227:CDS | 15.0% |
| !! | ATCCAACTTTCTTAATTTAA+TGG | - | chr4.3:85802171-85802190 | MS.gene60227:CDS | 20.0% |
| !! | ATTGATTACGTTTCAAAATT+TGG | - | chr4.3:85801672-85801691 | MS.gene60227:intron | 20.0% |
| !! | TACCATTAAATTAAGAAAGT+TGG | + | chr4.3:85802176-85802195 | None:intergenic | 20.0% |
| !!! | ATTTTGAAACGTAATCAATA+AGG | + | chr4.3:85801671-85801690 | None:intergenic | 20.0% |
| !!! | GAGTGTTTTAGTTTTAAATT+TGG | + | chr4.3:85801831-85801850 | None:intergenic | 20.0% |
| !!! | TGAAAATTGATTTTTGTATG+TGG | - | chr4.3:85801501-85801520 | MS.gene60227:intron | 20.0% |
| ! | AAAGATATGTGATTATCCTT+TGG | + | chr4.3:85801621-85801640 | None:intergenic | 25.0% |
| ! | GAAGAAAGAAATAAATCCAA+AGG | - | chr4.3:85801602-85801621 | MS.gene60227:intron | 25.0% |
| ! | GTGCAAAAAATTACATGATT+CGG | + | chr4.3:85801965-85801984 | None:intergenic | 25.0% |
| ! | GTTTCATCAGAAATTAAACT+TGG | - | chr4.3:85802329-85802348 | MS.gene60227:intron | 25.0% |
| ! | TTTGTGTTTGAACTTTGAAT+TGG | - | chr4.3:85802407-85802426 | MS.gene60227:intron | 25.0% |
| !! | ATGCTCACACATTTTAAATA+CGG | + | chr4.3:85802113-85802132 | None:intergenic | 25.0% |
| !! | CGTTTATGTTATCCTTTTTA+AGG | - | chr4.3:85801869-85801888 | MS.gene60227:intron | 25.0% |
| !! | CTCAACAATTTGTCATTTAT+CGG | - | chr4.3:85802358-85802377 | MS.gene60227:intron | 25.0% |
| !!! | AGATATGATTCACTGATTTT+AGG | + | chr4.3:85801109-85801128 | None:intergenic | 25.0% |
| ACAATGCATAATATATGCAG+AGG | - | chr4.3:85802064-85802083 | MS.gene60227:intron | 30.0% | |
| CGATAAATGACAAATTGTTG+AGG | + | chr4.3:85802360-85802379 | None:intergenic | 30.0% | |
| ! | GGATTTATTTCTTTCTTCCT+TGG | + | chr4.3:85801600-85801619 | None:intergenic | 30.0% |
| ! | TGCTCACACATTTTAAATAC+GGG | + | chr4.3:85802112-85802131 | None:intergenic | 30.0% |
| !! | TCCTTTTTAAGGATATGCAA+TGG | - | chr4.3:85801880-85801899 | MS.gene60227:intron | 30.0% |
| !!! | ATGTAATTTTTTGCACTGCA+CGG | - | chr4.3:85801969-85801988 | MS.gene60227:intron | 30.0% |
| !!! | TTTTTCTATGCGTCACTTTT+AGG | + | chr4.3:85801779-85801798 | None:intergenic | 30.0% |
| !!! | TTTTTTATCAAGGAGACTAG+TGG | + | chr4.3:85802139-85802158 | None:intergenic | 30.0% |
| ATCAGACTATACTTACCTCA+CGG | + | chr4.3:85802301-85802320 | None:intergenic | 35.0% | |
| ATCTCAATCATGAAACCATC+TGG | + | chr4.3:85802555-85802574 | None:intergenic | 35.0% | |
| CCATCAAAATCAAAAGAACC+AGG | + | chr4.3:85801206-85801225 | None:intergenic | 35.0% | |
| GATCTTATCCTCTAACTCTA+AGG | - | chr4.3:85801911-85801930 | MS.gene60227:intron | 35.0% | |
| GATTTCTACCAGAATGTTCT+TGG | - | chr4.3:85801161-85801180 | MS.gene60227:CDS | 35.0% | |
| GCCATTGCATATCCTTAAAA+AGG | + | chr4.3:85801884-85801903 | None:intergenic | 35.0% | |
| GGAGAAAAAGCTGAAAGAAA+TGG | - | chr4.3:85802461-85802480 | MS.gene60227:intron | 35.0% | |
| GGATAATCACATATCTTTCC+AGG | - | chr4.3:85801623-85801642 | MS.gene60227:intron | 35.0% | |
| GTTTAGTGAAAAATCTGACC+TGG | + | chr4.3:85801644-85801663 | None:intergenic | 35.0% | |
| TAAACGTGTCTATGATGACT+AGG | + | chr4.3:85801855-85801874 | None:intergenic | 35.0% | |
| TAGTCTATGTGAGCTTAACT+CGG | - | chr4.3:85802032-85802051 | MS.gene60227:intron | 35.0% | |
| TCTCAATCATGAAACCATCT+GGG | + | chr4.3:85802554-85802573 | None:intergenic | 35.0% | |
| TGCATAATATATGCAGAGGT+CGG | - | chr4.3:85802068-85802087 | MS.gene60227:intron | 35.0% | |
| TGTTAACCATTCGATTCTCA+GGG | - | chr4.3:85802247-85802266 | MS.gene60227:intron | 35.0% | |
| TTGGTTTCTTCCCAATAAGA+AGG | - | chr4.3:85801180-85801199 | MS.gene60227:CDS | 35.0% | |
| TTGTTAACCATTCGATTCTC+AGG | - | chr4.3:85802246-85802265 | MS.gene60227:intron | 35.0% | |
| ! | AGGATATGCAATGGCTTTTA+AGG | - | chr4.3:85801889-85801908 | MS.gene60227:intron | 35.0% |
| ! | GCTCACACATTTTAAATACG+GGG | + | chr4.3:85802111-85802130 | None:intergenic | 35.0% |
| !!! | CCTGGTTCTTTTGATTTTGA+TGG | - | chr4.3:85801203-85801222 | MS.gene60227:CDS | 35.0% |
| !!! | CTTTTGATTTTGATGGAGCA+TGG | - | chr4.3:85801210-85801229 | MS.gene60227:CDS | 35.0% |
| !!! | TCACTGATTTTAGGTGCAAA+GGG | + | chr4.3:85801100-85801119 | None:intergenic | 35.0% |
| !!! | TTCACTGATTTTAGGTGCAA+AGG | + | chr4.3:85801101-85801120 | None:intergenic | 35.0% |
| AAAGAACCAGGCCTTCTTAT+TGG | + | chr4.3:85801194-85801213 | None:intergenic | 40.0% | |
| AAGAACCAGGCCTTCTTATT+GGG | + | chr4.3:85801193-85801212 | None:intergenic | 40.0% | |
| AGCCAAATAGCCTGCAAATT+AGG | + | chr4.3:85801530-85801549 | None:intergenic | 40.0% | |
| AGGCTATTTGGCTATGGTAT+TGG | - | chr4.3:85801537-85801556 | MS.gene60227:intron | 40.0% | |
| ATTTGCAGGCTATTTGGCTA+TGG | - | chr4.3:85801531-85801550 | MS.gene60227:intron | 40.0% | |
| CATAATATATGCAGAGGTCG+GGG | - | chr4.3:85802070-85802089 | MS.gene60227:intron | 40.0% | |
| CTTCCTGTGATTCCATTAGT+TGG | - | chr4.3:85802591-85802610 | MS.gene60227:CDS | 40.0% | |
| GCATAATATATGCAGAGGTC+GGG | - | chr4.3:85802069-85802088 | MS.gene60227:intron | 40.0% | |
| GCATCGTGCAATTGTAGTAA+AGG | - | chr4.3:85802198-85802217 | MS.gene60227:CDS | 40.0% | |
| GGAAGAAACCAAGAACATTC+TGG | + | chr4.3:85801172-85801191 | None:intergenic | 40.0% | |
| TCACCAACTAATGGAATCAC+AGG | + | chr4.3:85802597-85802616 | None:intergenic | 40.0% | |
| TGAAGCAACCTTAGAGTTAG+AGG | + | chr4.3:85801922-85801941 | None:intergenic | 40.0% | |
| TTATGTTCGAGCAAGAGTAG+AGG | - | chr4.3:85802491-85802510 | MS.gene60227:intron | 40.0% | |
| ! | GACCTAATTTGCAGGCTATT+TGG | - | chr4.3:85801525-85801544 | MS.gene60227:intron | 40.0% |
| ! | GGAAAAGAGCTCTGGTAATT+CGG | - | chr4.3:85802268-85802287 | MS.gene60227:intron | 40.0% |
| ! | TATGTGGTGACCTAATTTGC+AGG | - | chr4.3:85801517-85801536 | MS.gene60227:intron | 40.0% |
| ! | TCTTTTCCCTGAGAATCGAA+TGG | + | chr4.3:85802256-85802275 | None:intergenic | 40.0% |
| ! | TGTGATTCCATTAGTTGGTG+AGG | - | chr4.3:85802596-85802615 | MS.gene60227:CDS | 40.0% |
| !! | CATTTTAAATACGGGGTGTC+TGG | + | chr4.3:85802104-85802123 | None:intergenic | 40.0% |
| !! | CTATTTTTCCATGACCCAGA+TGG | - | chr4.3:85802537-85802556 | MS.gene60227:intron | 40.0% |
| ATTCTCAGGGAAAAGAGCTC+TGG | - | chr4.3:85802260-85802279 | MS.gene60227:intron | 45.0% | |
| CTATGTGAGCTTAACTCGGT+TGG | - | chr4.3:85802036-85802055 | MS.gene60227:intron | 45.0% | |
| CTTGCTACCTCACCAACTAA+TGG | + | chr4.3:85802606-85802625 | None:intergenic | 45.0% | |
| TCATGAAACCATCTGGGTCA+TGG | + | chr4.3:85802548-85802567 | None:intergenic | 45.0% | |
| TTCTTCCCAATAAGAAGGCC+TGG | - | chr4.3:85801185-85801204 | MS.gene60227:CDS | 45.0% | |
| ! | TGAGCTTAACTCGGTTGGTA+GGG | - | chr4.3:85802041-85802060 | MS.gene60227:intron | 45.0% |
| !! | TCTTCCTTGGTAACGTCTCT+GGG | + | chr4.3:85801587-85801606 | None:intergenic | 45.0% |
| !! | TTCTTCCTTGGTAACGTCTC+TGG | + | chr4.3:85801588-85801607 | None:intergenic | 45.0% |
| !!! | TAATATATATTTTTTTATCA+AGG | + | chr4.3:85802149-85802168 | None:intergenic | 5.0% |
| AGAACCCAGAGACGTTACCA+AGG | - | chr4.3:85801580-85801599 | MS.gene60227:intron | 50.0% | |
| AGAGGAAGGTGGGATTGAAG+TGG | - | chr4.3:85802509-85802528 | MS.gene60227:intron | 50.0% | |
| GAGCAAGAGTAGAGGAAGGT+GGG | - | chr4.3:85802499-85802518 | MS.gene60227:intron | 50.0% | |
| GTGAGCTTAACTCGGTTGGT+AGG | - | chr4.3:85802040-85802059 | MS.gene60227:intron | 50.0% | |
| GTTCGAGCAAGAGTAGAGGA+AGG | - | chr4.3:85802495-85802514 | MS.gene60227:intron | 50.0% | |
| TAATTCGGAGTTCGACCGTG+AGG | - | chr4.3:85802283-85802302 | MS.gene60227:intron | 50.0% | |
| TGATGTGCAGTGTGAGAGCA+TGG | - | chr4.3:85802431-85802450 | MS.gene60227:intron | 50.0% | |
| ! | ATGTGCAGTGTGAGAGCATG+GGG | - | chr4.3:85802433-85802452 | MS.gene60227:intron | 50.0% |
| ! | GATGTGCAGTGTGAGAGCAT+GGG | - | chr4.3:85802432-85802451 | MS.gene60227:intron | 50.0% |
| CGAGCAAGAGTAGAGGAAGG+TGG | - | chr4.3:85802498-85802517 | MS.gene60227:intron | 55.0% | |
| ! | TGTGCAGTGTGAGAGCATGG+GGG | - | chr4.3:85802434-85802453 | MS.gene60227:intron | 55.0% |
| ! | GTGTGAGAGCATGGGGGCAG+TGG | - | chr4.3:85802440-85802459 | MS.gene60227:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 85801077 | 85802701 | 85801077 | ID=MS.gene60227 |
| chr4.3 | mRNA | 85801077 | 85802701 | 85801077 | ID=MS.gene60227.t1;Parent=MS.gene60227 |
| chr4.3 | exon | 85802547 | 85802701 | 85802547 | ID=MS.gene60227.t1.exon1;Parent=MS.gene60227.t1 |
| chr4.3 | CDS | 85802547 | 85802701 | 85802547 | ID=cds.MS.gene60227.t1;Parent=MS.gene60227.t1 |
| chr4.3 | exon | 85802134 | 85802239 | 85802134 | ID=MS.gene60227.t1.exon2;Parent=MS.gene60227.t1 |
| chr4.3 | CDS | 85802134 | 85802239 | 85802134 | ID=cds.MS.gene60227.t1;Parent=MS.gene60227.t1 |
| chr4.3 | exon | 85801077 | 85801337 | 85801077 | ID=MS.gene60227.t1.exon3;Parent=MS.gene60227.t1 |
| chr4.3 | CDS | 85801077 | 85801337 | 85801077 | ID=cds.MS.gene60227.t1;Parent=MS.gene60227.t1 |
| Gene Sequence |
| Protein sequence |
