Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027210.t1 | XP_022632118.1 | 88.5 | 383 | 35 | 2 | 1 | 374 | 1 | 383 | 1.80E-200 | 708.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027210.t1 | Q9SJW6 | 78.0 | 377 | 76 | 5 | 1 | 373 | 1 | 374 | 6.5e-175 | 614.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027210.t1 | A0A3Q0EJI2 | 88.5 | 383 | 35 | 2 | 1 | 374 | 1 | 383 | 1.3e-200 | 708.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene22023 | MS.gene027210 | PPI |
| MS.gene027210 | MS.gene070193 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027210.t1 | MTR_3g049070 | 99.467 | 375 | 2 | 0 | 1 | 375 | 1 | 375 | 0.0 | 774 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027210.t1 | AT2G31300 | 77.984 | 377 | 76 | 5 | 1 | 373 | 1 | 374 | 0.0 | 591 |
| MS.gene027210.t1 | AT2G30910 | 77.454 | 377 | 77 | 6 | 1 | 372 | 1 | 374 | 0.0 | 590 |
| MS.gene027210.t1 | AT2G30910 | 77.454 | 377 | 77 | 6 | 1 | 372 | 1 | 374 | 0.0 | 590 |
| MS.gene027210.t1 | AT2G30910 | 75.332 | 377 | 85 | 6 | 1 | 372 | 1 | 374 | 0.0 | 571 |
| MS.gene027210.t1 | AT2G31300 | 78.116 | 361 | 72 | 5 | 1 | 357 | 1 | 358 | 0.0 | 565 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTACATCTGATATGAAATT+TGG | 0.233687 | 3.4:-46241679 | MS.gene027210:CDS |
| CAGCATGATCAGGTAATATC+TGG | 0.263285 | 3.4:-46245315 | MS.gene027210:CDS |
| TATCAGTTCTCTGAAGCATT+TGG | 0.292881 | 3.4:-46239227 | MS.gene027210:intron |
| CTTCAGTGACAAGTGTTTCA+TGG | 0.301682 | 3.4:-46243081 | MS.gene027210:CDS |
| AATCACGGAACACGACATTC+TGG | 0.316967 | 3.4:+46240839 | None:intergenic |
| AAGAATTGCTGTATGGGATT+TGG | 0.350947 | 3.4:-46238786 | MS.gene027210:CDS |
| GTTTCTGAGAGAACGGTAAT+AGG | 0.350950 | 3.4:-46240684 | MS.gene027210:CDS |
| TGATGTGGGTCCTTCTCCTT+TGG | 0.364729 | 3.4:-46240863 | MS.gene027210:CDS |
| CGCAAGAATACCAACATTAT+CGG | 0.368715 | 3.4:+46243054 | None:intergenic |
| GTACCCATTCTGATCCTTCA+AGG | 0.371878 | 3.4:+46245097 | None:intergenic |
| CTCTCATCATCTTGGACATT+CGG | 0.378975 | 3.4:-46241326 | MS.gene027210:CDS |
| ATAGCATATACAAACAGTTT+TGG | 0.380254 | 3.4:+46244395 | None:intergenic |
| CTGCAGATGAAAGAGGAGTT+TGG | 0.391042 | 3.4:-46240629 | MS.gene027210:intron |
| ATCAGTTCTCTGAAGCATTT+GGG | 0.393609 | 3.4:-46239226 | MS.gene027210:intron |
| AGCTTGATCTCTCATCATCT+TGG | 0.394251 | 3.4:-46241334 | MS.gene027210:CDS |
| ATCGTTTCCCACACCATACT+TGG | 0.404007 | 3.4:+46239187 | None:intergenic |
| ATGTGAACTTCATTATTGTT+TGG | 0.410386 | 3.4:+46246482 | None:intergenic |
| GTGCTGCTCTTTGTGTTCAA+TGG | 0.424300 | 3.4:-46245047 | MS.gene027210:CDS |
| GTAGATTGGTCGAAGACAAA+TGG | 0.453328 | 3.4:-46246457 | MS.gene027210:CDS |
| ACACAGGCACTCACGAATTC+CGG | 0.465538 | 3.4:+46245244 | None:intergenic |
| GTATCATCTGGATCAAGATA+TGG | 0.468909 | 3.4:-46240414 | MS.gene027210:CDS |
| TTCCCACACCATACTTGGAT+TGG | 0.478929 | 3.4:+46239192 | None:intergenic |
| TGAAGTTCACATCTGTAGAT+TGG | 0.482915 | 3.4:-46246471 | MS.gene027210:CDS |
| AGGTAATATCTGGGATAGAC+TGG | 0.489400 | 3.4:-46245305 | MS.gene027210:CDS |
| ATCATACCTCTTGGTGAGCA+TGG | 0.490694 | 3.4:-46238960 | MS.gene027210:CDS |
| TGTGTTCAATGGAGTCCAAA+AGG | 0.493426 | 3.4:-46245036 | MS.gene027210:intron |
| TGTAGTTCTATCATACCTCT+TGG | 0.495135 | 3.4:-46238969 | MS.gene027210:intron |
| ATCACGGAACACGACATTCT+GGG | 0.498335 | 3.4:+46240840 | None:intergenic |
| TAGATTGGTCGAAGACAAAT+GGG | 0.498387 | 3.4:-46246456 | MS.gene027210:CDS |
| AACAAGTTTGCAGTGGGAAG+TGG | 0.503387 | 3.4:-46244420 | MS.gene027210:CDS |
| AAAGGTGCATGTTCTTCAAA+AGG | 0.504464 | 3.4:-46246432 | MS.gene027210:intron |
| AATGCATCACTTGTCACGCT+TGG | 0.505275 | 3.4:-46248630 | MS.gene027210:CDS |
| GAGCGGAAGACAGTATCATC+TGG | 0.508128 | 3.4:-46240426 | MS.gene027210:CDS |
| ATTATGAGCAGGAAAACAAC+TGG | 0.512153 | 3.4:-46244374 | MS.gene027210:intron |
| TGGATGGAAGAATTGCTGTA+TGG | 0.522720 | 3.4:-46238793 | MS.gene027210:CDS |
| GCAAGAATACCAACATTATC+GGG | 0.523262 | 3.4:+46243055 | None:intergenic |
| TACCAACTCTTGTTATCCTT+AGG | 0.525803 | 3.4:-46245077 | MS.gene027210:CDS |
| AGAGTGGTTACTAGATTGAT+CGG | 0.525825 | 3.4:+46248603 | None:intergenic |
| GCACTCACGAATTCCGGTCG+TGG | 0.527821 | 3.4:+46245250 | None:intergenic |
| TTGTATATGCTATTATGAGC+AGG | 0.529086 | 3.4:-46244385 | MS.gene027210:CDS |
| AGATATGTCTGGAACCTTGA+AGG | 0.529442 | 3.4:-46245111 | MS.gene027210:CDS |
| GGATGGAAGAATTGCTGTAT+GGG | 0.529737 | 3.4:-46238792 | MS.gene027210:CDS |
| ATTATTTGTTTCTGAGAGAA+CGG | 0.529928 | 3.4:-46240691 | MS.gene027210:CDS |
| GAGCAGCACGGTTTAGCCTA+AGG | 0.537294 | 3.4:+46245061 | None:intergenic |
| GAACCTTGAAGGATCAGAAT+GGG | 0.538758 | 3.4:-46245100 | MS.gene027210:CDS |
| TACTCTGCACTTCCCATCCG+TGG | 0.545713 | 3.4:+46241839 | None:intergenic |
| TTTCTTACCTACATAAGCTA+GGG | 0.545926 | 3.4:+46241277 | None:intergenic |
| CACTCACGAATTCCGGTCGT+GGG | 0.548871 | 3.4:+46245251 | None:intergenic |
| AGCCTAAGGATAACAAGAGT+TGG | 0.549485 | 3.4:+46245075 | None:intergenic |
| ATGGGATTTGGAAAATGAGC+AGG | 0.551586 | 3.4:-46238774 | MS.gene027210:CDS |
| GGTCGAAGACAAATGGGAAA+AGG | 0.558159 | 3.4:-46246450 | MS.gene027210:CDS |
| GCAGAAAACAAGTTTGCAGT+GGG | 0.561048 | 3.4:-46244426 | MS.gene027210:intron |
| CCTGAAACTACAGGATTGGA+TGG | 0.561224 | 3.4:-46238809 | MS.gene027210:intron |
| GAGTGGTTACTAGATTGATC+GGG | 0.563409 | 3.4:+46248604 | None:intergenic |
| GGCAGAAAACAAGTTTGCAG+TGG | 0.567517 | 3.4:-46244427 | MS.gene027210:intron |
| GGAACCTTGAAGGATCAGAA+TGG | 0.576474 | 3.4:-46245101 | MS.gene027210:CDS |
| AGCATGATCAGGTAATATCT+GGG | 0.579626 | 3.4:-46245314 | MS.gene027210:CDS |
| TAGTTACTTCATCCCACGAC+CGG | 0.580689 | 3.4:-46245263 | MS.gene027210:CDS |
| GGCCAATCCAAGTATGGTGT+GGG | 0.581786 | 3.4:-46239194 | MS.gene027210:CDS |
| ACATTCTGGGCCAAAGGAGA+AGG | 0.582007 | 3.4:+46240853 | None:intergenic |
| AATAAGCTTATAATTCAAGA+AGG | 0.583387 | 3.4:+46238750 | None:intergenic |
| GAGAGAACGGTAATAGGTGT+GGG | 0.585251 | 3.4:-46240678 | MS.gene027210:CDS |
| CTTGCGACAACATCCACGGA+TGG | 0.586607 | 3.4:-46241852 | MS.gene027210:CDS |
| TGAGAGAACGGTAATAGGTG+TGG | 0.589873 | 3.4:-46240679 | MS.gene027210:CDS |
| TGGATCAAGATATGGCTCAC+AGG | 0.591647 | 3.4:-46240406 | MS.gene027210:intron |
| GGCAATACCCTAGCTTATGT+AGG | 0.594026 | 3.4:-46241284 | MS.gene027210:intron |
| TGGCCAATCCAAGTATGGTG+TGG | 0.598684 | 3.4:-46239195 | MS.gene027210:CDS |
| TTGCGACAACATCCACGGAT+GGG | 0.605771 | 3.4:-46241851 | MS.gene027210:CDS |
| TAAGCTAGGGTATTGCCACT+TGG | 0.608759 | 3.4:+46241290 | None:intergenic |
| AACACGACATTCTGGGCCAA+AGG | 0.611884 | 3.4:+46240847 | None:intergenic |
| TCTTCTTGCGACAACATCCA+CGG | 0.612313 | 3.4:-46241856 | MS.gene027210:CDS |
| ATCTGATATGAAATTTGGAG+AGG | 0.617391 | 3.4:-46241674 | MS.gene027210:intron |
| GGGATATGATTGTAACCCAA+TGG | 0.637775 | 3.4:-46240658 | MS.gene027210:CDS |
| CATCACGGAGAGGCAAATCA+CGG | 0.642366 | 3.4:+46240824 | None:intergenic |
| TTGTCAGGTATCTTGGAGAG+CGG | 0.646625 | 3.4:-46240443 | MS.gene027210:CDS |
| CTTGGACATTCGGAGTCAAG+TGG | 0.649894 | 3.4:-46241316 | MS.gene027210:CDS |
| ATTGAACACAAAGAGCAGCA+CGG | 0.658833 | 3.4:+46245049 | None:intergenic |
| AATGTTCCATGCTCACCAAG+AGG | 0.660956 | 3.4:+46238954 | None:intergenic |
| TGCTGACCCACATCACGGAG+AGG | 0.674971 | 3.4:+46240814 | None:intergenic |
| GTGAGCATGGAACATTGACA+AGG | 0.696012 | 3.4:-46238947 | MS.gene027210:CDS |
| GGAGTCAAGTGGTCACCAAG+TGG | 0.703568 | 3.4:-46241305 | MS.gene027210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 46238757 | 46248683 | 46238757 | ID=MS.gene027210 |
| chr3.4 | mRNA | 46238757 | 46248683 | 46238757 | ID=MS.gene027210.t1;Parent=MS.gene027210 |
| chr3.4 | exon | 46248614 | 46248683 | 46248614 | ID=MS.gene027210.t1.exon1;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46248614 | 46248683 | 46248614 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46246433 | 46246518 | 46246433 | ID=MS.gene027210.t1.exon2;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46246433 | 46246518 | 46246433 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46245258 | 46245334 | 46245258 | ID=MS.gene027210.t1.exon3;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46245258 | 46245334 | 46245258 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46245037 | 46245131 | 46245037 | ID=MS.gene027210.t1.exon4;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46245037 | 46245131 | 46245037 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46244375 | 46244444 | 46244375 | ID=MS.gene027210.t1.exon5;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46244375 | 46244444 | 46244375 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46243069 | 46243138 | 46243069 | ID=MS.gene027210.t1.exon6;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46243069 | 46243138 | 46243069 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46241807 | 46241880 | 46241807 | ID=MS.gene027210.t1.exon7;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46241807 | 46241880 | 46241807 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46241675 | 46241714 | 46241675 | ID=MS.gene027210.t1.exon8;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46241675 | 46241714 | 46241675 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46241285 | 46241366 | 46241285 | ID=MS.gene027210.t1.exon9;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46241285 | 46241366 | 46241285 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46240825 | 46240913 | 46240825 | ID=MS.gene027210.t1.exon10;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46240825 | 46240913 | 46240825 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46240630 | 46240715 | 46240630 | ID=MS.gene027210.t1.exon11;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46240630 | 46240715 | 46240630 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46240407 | 46240470 | 46240407 | ID=MS.gene027210.t1.exon12;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46240407 | 46240470 | 46240407 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46239137 | 46239243 | 46239137 | ID=MS.gene027210.t1.exon13;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46239137 | 46239243 | 46239137 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46238931 | 46238986 | 46238931 | ID=MS.gene027210.t1.exon14;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46238931 | 46238986 | 46238931 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| chr3.4 | exon | 46238757 | 46238818 | 46238757 | ID=MS.gene027210.t1.exon15;Parent=MS.gene027210.t1 |
| chr3.4 | CDS | 46238757 | 46238818 | 46238757 | ID=cds.MS.gene027210.t1;Parent=MS.gene027210.t1 |
| Gene Sequence |
| Protein sequence |
