Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070193.t1 | XP_003599925.1 | 99.7 | 375 | 1 | 0 | 1 | 375 | 1 | 375 | 8.40E-182 | 646.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070193.t1 | Q9SJW6 | 65.3 | 377 | 124 | 5 | 1 | 373 | 1 | 374 | 5.4e-137 | 488.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070193.t1 | G7IZZ4 | 99.7 | 375 | 1 | 0 | 1 | 375 | 1 | 375 | 6.1e-182 | 646.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene070193 | 0.807429 | 5.08E-50 | -1.69E-46 |
| MS.gene049254 | MS.gene070193 | 0.81328 | 2.77E-51 | -1.69E-46 |
| MS.gene049666 | MS.gene070193 | 0.803887 | 2.81E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene070193 | 0.817287 | 3.57E-52 | -1.69E-46 |
| MS.gene050559 | MS.gene070193 | 0.801168 | 1.02E-48 | -1.69E-46 |
| MS.gene050561 | MS.gene070193 | 0.804337 | 2.27E-49 | -1.69E-46 |
| MS.gene050562 | MS.gene070193 | 0.806222 | 9.13E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene070193 | 0.82425 | 8.94E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene070193 | 0.817624 | 2.99E-52 | -1.69E-46 |
| MS.gene05191 | MS.gene070193 | 0.810196 | 1.30E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene070193 | 0.804883 | 1.74E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene070193 | 0.816749 | 4.71E-52 | -1.69E-46 |
| MS.gene053998 | MS.gene070193 | 0.814755 | 1.31E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene070193 | 0.807353 | 5.27E-50 | -1.69E-46 |
| MS.gene054991 | MS.gene070193 | 0.81289 | 3.38E-51 | -1.69E-46 |
| MS.gene056050 | MS.gene070193 | 0.834839 | 2.38E-56 | -1.69E-46 |
| MS.gene056174 | MS.gene070193 | 0.831394 | 1.71E-55 | -1.69E-46 |
| MS.gene05686 | MS.gene070193 | 0.832771 | 7.82E-56 | -1.69E-46 |
| MS.gene05690 | MS.gene070193 | 0.805757 | 1.14E-49 | -1.69E-46 |
| MS.gene057915 | MS.gene070193 | 0.80633 | 8.67E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene070193 | 0.806981 | 6.32E-50 | -1.69E-46 |
| MS.gene05897 | MS.gene070193 | 0.807336 | 5.31E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene070193 | 0.843571 | 1.29E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene22023 | MS.gene070193 | PPI |
| MS.gene027210 | MS.gene070193 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070193.t1 | MTR_3g049070 | 99.733 | 375 | 1 | 0 | 1 | 375 | 1 | 375 | 0.0 | 778 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070193.t1 | AT2G31300 | 78.249 | 377 | 75 | 5 | 1 | 373 | 1 | 374 | 0.0 | 595 |
| MS.gene070193.t1 | AT2G30910 | 77.719 | 377 | 76 | 6 | 1 | 372 | 1 | 374 | 0.0 | 594 |
| MS.gene070193.t1 | AT2G30910 | 77.719 | 377 | 76 | 6 | 1 | 372 | 1 | 374 | 0.0 | 594 |
| MS.gene070193.t1 | AT2G30910 | 75.597 | 377 | 84 | 6 | 1 | 372 | 1 | 374 | 0.0 | 574 |
| MS.gene070193.t1 | AT2G31300 | 78.393 | 361 | 71 | 5 | 1 | 357 | 1 | 358 | 0.0 | 568 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTACATCTGATATGAAATT+TGG | 0.233687 | 3.2:-41721505 | MS.gene070193:CDS |
| CAGCATGATCAGGTAATATC+TGG | 0.263285 | 3.2:-41724398 | MS.gene070193:CDS |
| TATCAGTTCTCTGAAGCATT+TGG | 0.292881 | 3.2:-41719063 | MS.gene070193:intron |
| CTTCAGTGACAAGTGTTTCA+TGG | 0.301682 | 3.2:-41722629 | MS.gene070193:CDS |
| AATCACGGAACACGACATTC+TGG | 0.316967 | 3.2:+41720675 | None:intergenic |
| AAGAATTGCTGTATGGGATT+TGG | 0.350947 | 3.2:-41718625 | MS.gene070193:CDS |
| GTTTCTGAGAGAACGGTAAT+AGG | 0.350950 | 3.2:-41720520 | MS.gene070193:CDS |
| TGATGTGGGTCCTTCTCCTT+TGG | 0.364729 | 3.2:-41720699 | MS.gene070193:CDS |
| GTACCCATTCTGATCCTTCA+AGG | 0.371878 | 3.2:+41724180 | None:intergenic |
| CTCTCATCATCTTGGACATT+CGG | 0.378975 | 3.2:-41721162 | MS.gene070193:CDS |
| CTGCAGATGAAAGAGGAGTT+TGG | 0.391042 | 3.2:-41720465 | MS.gene070193:intron |
| ATCAGTTCTCTGAAGCATTT+GGG | 0.393609 | 3.2:-41719062 | MS.gene070193:intron |
| AGCTTGATCTCTCATCATCT+TGG | 0.394251 | 3.2:-41721170 | MS.gene070193:CDS |
| ATAGCATATACAGACAGTTT+TGG | 0.396415 | 3.2:+41723522 | None:intergenic |
| ATGTGAACTTCATTATTGTT+TGG | 0.396486 | 3.2:+41725480 | None:intergenic |
| ATCGTTTCCCACACCATACT+TGG | 0.404007 | 3.2:+41719023 | None:intergenic |
| AGCAAGAATACCAACATTAT+CGG | 0.404028 | 3.2:+41722602 | None:intergenic |
| GTGCTGCTCTTTGTGTTCAA+TGG | 0.424300 | 3.2:-41724130 | MS.gene070193:CDS |
| ATAGATTGGTCGAAGACAAA+TGG | 0.455768 | 3.2:-41725455 | MS.gene070193:CDS |
| GTGTTCTTGCTAGATATGTC+TGG | 0.457083 | 3.2:-41724205 | MS.gene070193:intron |
| TGAAGTTCACATCTATAGAT+TGG | 0.461455 | 3.2:-41725469 | MS.gene070193:CDS |
| ACACAGGCACTCACGAATTC+CGG | 0.465538 | 3.2:+41724327 | None:intergenic |
| GTATCATCTGGATCAAGATA+TGG | 0.468909 | 3.2:-41720250 | MS.gene070193:CDS |
| TTCCCACACCATACTTGGAT+TGG | 0.478929 | 3.2:+41719028 | None:intergenic |
| AGGTAATATCTGGGATAGAC+TGG | 0.489400 | 3.2:-41724388 | MS.gene070193:CDS |
| ATCATACCTCTTGGTGAGCA+TGG | 0.490694 | 3.2:-41718796 | MS.gene070193:CDS |
| TGTGTTCAATGGAGTCCAAA+AGG | 0.493426 | 3.2:-41724119 | MS.gene070193:intron |
| TGTAGTTCTATCATACCTCT+TGG | 0.495135 | 3.2:-41718805 | MS.gene070193:intron |
| ATCACGGAACACGACATTCT+GGG | 0.498335 | 3.2:+41720676 | None:intergenic |
| TAGATTGGTCGAAGACAAAT+GGG | 0.500826 | 3.2:-41725454 | MS.gene070193:CDS |
| GCAAGAATACCAACATTATC+GGG | 0.500863 | 3.2:+41722603 | None:intergenic |
| AACAAGTTTGCAGTGGGAAG+TGG | 0.503387 | 3.2:-41723547 | MS.gene070193:CDS |
| AAAGGTGCATGTTCTTCAAA+AGG | 0.504464 | 3.2:-41725430 | MS.gene070193:intron |
| AATGCATCACTTGTCACGCT+TGG | 0.505275 | 3.2:-41728313 | MS.gene070193:CDS |
| GAGCGGAAGACAGTATCATC+TGG | 0.508128 | 3.2:-41720262 | MS.gene070193:CDS |
| ATTATGAGCAGGAAAACAAC+TGG | 0.512153 | 3.2:-41723501 | MS.gene070193:intron |
| TGGATGGAAGAATTGCTGTA+TGG | 0.522720 | 3.2:-41718632 | MS.gene070193:CDS |
| TACCAACTCTTGTTATCCTT+AGG | 0.525803 | 3.2:-41724160 | MS.gene070193:CDS |
| AGAGTGGTTACTAGATTGAT+CGG | 0.525825 | 3.2:+41728286 | None:intergenic |
| GCACTCACGAATTCCGGTCG+TGG | 0.527821 | 3.2:+41724333 | None:intergenic |
| AGATATGTCTGGAACCTTGA+AGG | 0.529442 | 3.2:-41724194 | MS.gene070193:CDS |
| GGATGGAAGAATTGCTGTAT+GGG | 0.529737 | 3.2:-41718631 | MS.gene070193:CDS |
| ATTATTTGTTTCTGAGAGAA+CGG | 0.529928 | 3.2:-41720527 | MS.gene070193:CDS |
| CTGTATATGCTATTATGAGC+AGG | 0.530365 | 3.2:-41723512 | MS.gene070193:CDS |
| GAGCAGCACGGTTTAGCCTA+AGG | 0.537294 | 3.2:+41724144 | None:intergenic |
| TTTGAAACTACAGGATTGGA+TGG | 0.538066 | 3.2:-41718648 | MS.gene070193:intron |
| GAACCTTGAAGGATCAGAAT+GGG | 0.538758 | 3.2:-41724183 | MS.gene070193:CDS |
| TACTCTGCACTTCCCATCCG+TGG | 0.545713 | 3.2:+41721665 | None:intergenic |
| CACTCACGAATTCCGGTCGT+GGG | 0.548871 | 3.2:+41724334 | None:intergenic |
| AGCCTAAGGATAACAAGAGT+TGG | 0.549485 | 3.2:+41724158 | None:intergenic |
| ATGGGATTTGGAAAATGAGC+AGG | 0.551586 | 3.2:-41718613 | MS.gene070193:CDS |
| GGTCGAAGACAAATGGGAAA+AGG | 0.558159 | 3.2:-41725448 | MS.gene070193:CDS |
| GCAGAAAACAAGTTTGCAGT+GGG | 0.561048 | 3.2:-41723553 | MS.gene070193:intron |
| GAGTGGTTACTAGATTGATC+GGG | 0.563409 | 3.2:+41728287 | None:intergenic |
| GGCAATACTCTAGCTTATGT+AGG | 0.567396 | 3.2:-41721120 | MS.gene070193:intron |
| GGCAGAAAACAAGTTTGCAG+TGG | 0.567517 | 3.2:-41723554 | MS.gene070193:intron |
| GGAACCTTGAAGGATCAGAA+TGG | 0.576474 | 3.2:-41724184 | MS.gene070193:CDS |
| CTTGCTACAACATCCACGGA+TGG | 0.576770 | 3.2:-41721678 | MS.gene070193:CDS |
| AGCATGATCAGGTAATATCT+GGG | 0.579626 | 3.2:-41724397 | MS.gene070193:CDS |
| TAGTTACTTCATCCCACGAC+CGG | 0.580689 | 3.2:-41724346 | MS.gene070193:CDS |
| GGCCAATCCAAGTATGGTGT+GGG | 0.581786 | 3.2:-41719030 | MS.gene070193:CDS |
| ACATTCTGGGCCAAAGGAGA+AGG | 0.582007 | 3.2:+41720689 | None:intergenic |
| AATAAGCTTATAATTCAAGA+AGG | 0.583387 | 3.2:+41718589 | None:intergenic |
| GAGAGAACGGTAATAGGTGT+GGG | 0.585251 | 3.2:-41720514 | MS.gene070193:CDS |
| TGAGAGAACGGTAATAGGTG+TGG | 0.589873 | 3.2:-41720515 | MS.gene070193:CDS |
| TGGATCAAGATATGGCTCAC+AGG | 0.591647 | 3.2:-41720242 | MS.gene070193:intron |
| TGGCCAATCCAAGTATGGTG+TGG | 0.598684 | 3.2:-41719031 | MS.gene070193:CDS |
| TTGCTACAACATCCACGGAT+GGG | 0.604516 | 3.2:-41721677 | MS.gene070193:CDS |
| AACACGACATTCTGGGCCAA+AGG | 0.611884 | 3.2:+41720683 | None:intergenic |
| ATCTGATATGAAATTTGGAG+AGG | 0.617391 | 3.2:-41721500 | MS.gene070193:intron |
| TCTTCTTGCTACAACATCCA+CGG | 0.624767 | 3.2:-41721682 | MS.gene070193:CDS |
| GGGATATGATTGTAACCCAA+TGG | 0.637775 | 3.2:-41720494 | MS.gene070193:CDS |
| CATCACGGAGAGGCAAATCA+CGG | 0.642366 | 3.2:+41720660 | None:intergenic |
| TTGTCAGGTATCTTGGAGAG+CGG | 0.646625 | 3.2:-41720279 | MS.gene070193:CDS |
| CTTGGACATTCGGAGTCAAG+TGG | 0.649894 | 3.2:-41721152 | MS.gene070193:CDS |
| TAAGCTAGAGTATTGCCACT+TGG | 0.653319 | 3.2:+41721126 | None:intergenic |
| ATTGAACACAAAGAGCAGCA+CGG | 0.658833 | 3.2:+41724132 | None:intergenic |
| AATGTTCCATGCTCACCAAG+AGG | 0.660956 | 3.2:+41718790 | None:intergenic |
| TGCTGACCCACATCACGGAG+AGG | 0.674971 | 3.2:+41720650 | None:intergenic |
| GTGAGCATGGAACATTGACA+AGG | 0.696012 | 3.2:-41718783 | MS.gene070193:CDS |
| GGAGTCAAGTGGTCACCAAG+TGG | 0.703568 | 3.2:-41721141 | MS.gene070193:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 41718596 | 41728366 | 41718596 | ID=MS.gene070193 |
| chr3.2 | mRNA | 41718596 | 41728366 | 41718596 | ID=MS.gene070193.t1;Parent=MS.gene070193 |
| chr3.2 | exon | 41728297 | 41728366 | 41728297 | ID=MS.gene070193.t1.exon1;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41728297 | 41728366 | 41728297 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41725431 | 41725516 | 41725431 | ID=MS.gene070193.t1.exon2;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41725431 | 41725516 | 41725431 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41724341 | 41724417 | 41724341 | ID=MS.gene070193.t1.exon3;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41724341 | 41724417 | 41724341 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41724120 | 41724214 | 41724120 | ID=MS.gene070193.t1.exon4;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41724120 | 41724214 | 41724120 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41723502 | 41723571 | 41723502 | ID=MS.gene070193.t1.exon5;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41723502 | 41723571 | 41723502 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41722617 | 41722686 | 41722617 | ID=MS.gene070193.t1.exon6;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41722617 | 41722686 | 41722617 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41721633 | 41721706 | 41721633 | ID=MS.gene070193.t1.exon7;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41721633 | 41721706 | 41721633 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41721501 | 41721540 | 41721501 | ID=MS.gene070193.t1.exon8;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41721501 | 41721540 | 41721501 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41721121 | 41721202 | 41721121 | ID=MS.gene070193.t1.exon9;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41721121 | 41721202 | 41721121 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41720661 | 41720749 | 41720661 | ID=MS.gene070193.t1.exon10;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41720661 | 41720749 | 41720661 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41720466 | 41720551 | 41720466 | ID=MS.gene070193.t1.exon11;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41720466 | 41720551 | 41720466 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41720243 | 41720306 | 41720243 | ID=MS.gene070193.t1.exon12;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41720243 | 41720306 | 41720243 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41718973 | 41719079 | 41718973 | ID=MS.gene070193.t1.exon13;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41718973 | 41719079 | 41718973 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41718767 | 41718822 | 41718767 | ID=MS.gene070193.t1.exon14;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41718767 | 41718822 | 41718767 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| chr3.2 | exon | 41718596 | 41718657 | 41718596 | ID=MS.gene070193.t1.exon15;Parent=MS.gene070193.t1 |
| chr3.2 | CDS | 41718596 | 41718657 | 41718596 | ID=cds.MS.gene070193.t1;Parent=MS.gene070193.t1 |
| Gene Sequence |
| Protein sequence |
