AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027467

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027467.t1 MTR_4g045667 97.379 496 13 0 1 496 1 496 0.0 1009
MS.gene027467.t1 MTR_2g089030 73.306 487 130 0 8 494 81 567 0.0 780
MS.gene027467.t1 MTR_5g013620 50.575 435 198 4 11 442 87 507 5.44e-158 460
MS.gene027467.t1 MTR_5g013640 50.577 433 197 4 13 442 87 505 3.60e-157 457
MS.gene027467.t1 MTR_4g099110 51.501 433 199 5 13 442 78 502 1.31e-155 454
MS.gene027467.t1 MTR_7g077410 49.886 439 208 6 12 445 264 695 1.40e-149 444
MS.gene027467.t1 MTR_5g026210 45.547 393 210 3 12 402 217 607 5.29e-130 392
MS.gene027467.t1 MTR_6g477780 52.616 344 154 6 104 442 9 348 7.15e-121 359
MS.gene027467.t1 MTR_8g099775 37.671 438 260 8 14 444 80 511 3.34e-102 317
MS.gene027467.t1 MTR_5g026210 46.599 294 151 2 12 304 217 505 7.85e-98 305
MS.gene027467.t1 MTR_2g020240 38.196 377 219 6 13 383 83 451 5.90e-90 285
MS.gene027467.t1 MTR_5g026210 47.951 244 126 1 12 254 217 460 8.95e-85 269
MS.gene027467.t1 MTR_2g020240 39.441 322 182 5 67 383 3 316 5.20e-80 255
MS.gene027467.t1 MTR_6g477770 50.538 93 46 0 13 105 106 198 2.66e-29 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027467.t1 AT4G15210 68.750 496 154 1 2 496 3 498 0.0 741
MS.gene027467.t1 AT2G32290 67.206 494 159 2 2 494 67 558 0.0 703
MS.gene027467.t1 AT4G15210 69.213 445 137 0 52 496 1 445 0.0 677
MS.gene027467.t1 AT4G15210 69.213 445 137 0 52 496 1 445 0.0 677
MS.gene027467.t1 AT4G15210 70.833 408 118 1 2 408 3 410 0.0 630
MS.gene027467.t1 AT4G00490 52.403 437 198 5 13 445 95 525 1.03e-164 477
MS.gene027467.t1 AT4G17090 50.808 433 202 4 13 442 86 510 5.91e-162 470
MS.gene027467.t1 AT3G23920 50.909 440 209 6 13 447 106 543 6.74e-159 463
MS.gene027467.t1 AT2G45880 46.925 439 221 5 12 445 247 678 1.43e-139 418
MS.gene027467.t1 AT5G45300 41.514 436 247 5 12 445 252 681 1.04e-119 367
MS.gene027467.t1 AT5G45300 41.514 436 247 5 12 445 254 683 1.07e-119 367
MS.gene027467.t1 AT5G45300 41.514 436 247 5 12 445 268 697 1.71e-119 366
MS.gene027467.t1 AT5G55700 40.268 447 245 9 13 445 92 530 1.90e-112 343
MS.gene027467.t1 AT5G55700 41.691 343 182 8 113 445 33 367 2.10e-85 268
MS.gene027467.t1 AT5G18670 32.880 441 277 6 1 439 75 498 2.98e-74 244

Find 112 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGGAATTGGAGAGTTTC+AGG 0.170209 4.2:-54455554 MS.gene027467:intron
ACACCAATCGCTCCGGTATT+AGG 0.274428 4.2:-54456124 MS.gene027467:CDS
GGATGGCAATTTCCTGGAAT+TGG 0.314241 4.2:-54455565 MS.gene027467:CDS
AGAACATCATCAGGAATCTT+TGG 0.317642 4.2:+54454422 None:intergenic
ACTCCATACATCCGAAGTTT+TGG 0.319950 4.2:+54454673 None:intergenic
CTACATTTCCTCCACATTGA+TGG 0.320030 4.2:+54456216 None:intergenic
TGAAGCTAATAAAGCTTTCC+TGG 0.337953 4.2:-54455222 MS.gene027467:CDS
TTGAAGAATTCGGTAGATTC+TGG 0.345794 4.2:+54455335 None:intergenic
AGTTGTTCTTTAAGTCCATC+TGG 0.346354 4.2:+54456398 None:intergenic
GATAACAAACCCATCTTTCA+TGG 0.347915 4.2:-54456074 MS.gene027467:CDS
ATTGGTTAGAATACCAAGTT+AGG 0.349643 4.2:+54455273 None:intergenic
AGAGAAAACATTGAAGTTGC+TGG 0.361074 4.2:-54454784 MS.gene027467:CDS
GATCACCATGATTCAGCAAT+TGG 0.366811 4.2:+54455255 None:intergenic
GAAACAGACATGAAAGTTGA+TGG 0.371042 4.2:-54454356 MS.gene027467:CDS
ACTGGGAGCATGACATAAAC+TGG 0.372512 4.2:+54456551 None:intergenic
AGCCAATACCGCCGTTCCCC+TGG 0.375627 4.2:-54454385 MS.gene027467:CDS
CGATTGGTGTAGAAGATATC+AGG 0.378414 4.2:+54456137 None:intergenic
ACCTGAAACTCTCCAATTCC+AGG 0.378431 4.2:+54455553 None:intergenic
AATCAAGGATGGCAATTTCC+TGG 0.389845 4.2:-54455571 MS.gene027467:CDS
CATGTCTGTTTCTGAATCCC+AGG 0.393293 4.2:+54454367 None:intergenic
TCCCAGGGGAACGGCGGTAT+TGG 0.394469 4.2:+54454383 None:intergenic
AAGCTTTATTAGCTTCATCT+AGG 0.401337 4.2:+54455228 None:intergenic
TTGTACGTGCCAGCGTCATC+TGG 0.406376 4.2:+54455365 None:intergenic
GTCTTGCATTCAGTATGATT+TGG 0.408923 4.2:+54454722 None:intergenic
AAAGCAGCTGCAGCGAGTGC+TGG 0.411824 4.2:-54455407 MS.gene027467:CDS
TTTGGTCCTGATCGCTTTAA+CGG 0.417754 4.2:+54454440 None:intergenic
CTGGAGAAAATGCACTTTCA+AGG 0.424053 4.2:-54454765 MS.gene027467:CDS
AGAATTTCCCTTTCTCAGTA+AGG 0.426702 4.2:+54455297 None:intergenic
AAGGGAAATTCTTCCTAACT+TGG 0.434705 4.2:-54455286 MS.gene027467:CDS
GTGAGTTCTGCAGCGTGATT+CGG 0.440264 4.2:+54455061 None:intergenic
GGCCGTTCTTCCATGAAAGA+TGG 0.440893 4.2:+54456064 None:intergenic
CTATGTATAATAAAGGTCTC+TGG 0.447912 4.2:-54455106 MS.gene027467:intron
CACCAATGCTCAGATATTCC+TGG 0.447983 4.2:+54456099 None:intergenic
GGCACATACCTTACTGAGAA+AGG 0.452438 4.2:-54455305 MS.gene027467:CDS
TCTCCAAAACTTCGGATGTA+TGG 0.458600 4.2:-54454676 MS.gene027467:CDS
AGGGACCTAAGGAGTATGAT+TGG 0.463127 4.2:-54456307 MS.gene027467:CDS
CGAGTGCTGGCCATTCTGAA+TGG 0.465242 4.2:-54455394 MS.gene027467:CDS
CATCAATGTGGAGGAAATGT+AGG 0.469434 4.2:-54456215 MS.gene027467:CDS
GCACTCCAATCATACTCCTT+AGG 0.478043 4.2:+54456302 None:intergenic
GGTAAAGATGCATGCAGATC+AGG 0.484424 4.2:-54454584 MS.gene027467:intron
GCTTCATTAAGAACATCATC+AGG 0.484558 4.2:+54454413 None:intergenic
CTAATTTGACTTTACATCCC+AGG 0.486962 4.2:+54455204 None:intergenic
GTGTTATGACAAATATTTGA+AGG 0.488663 4.2:-54455438 MS.gene027467:CDS
ATAGACATTGAAGTCGGTCT+TGG 0.489311 4.2:-54455631 MS.gene027467:CDS
ACCGAATTCTTCAAAACAAA+CGG 0.489789 4.2:-54455326 MS.gene027467:CDS
CAAAGTTTAACTCACCTCAA+TGG 0.489937 4.2:+54456043 None:intergenic
TCAAGCACCCACTTTGGTAA+TGG 0.492462 4.2:+54456176 None:intergenic
CCGTCTTATCCGCAGAATCA+AGG 0.494530 4.2:-54455586 MS.gene027467:CDS
GGTCCTGATCGCTTTAACGG+TGG 0.496755 4.2:+54454443 None:intergenic
ACCACAGAAACTTGTTCAGC+AGG 0.503110 4.2:-54454903 MS.gene027467:intron
CCGTCTTTGTTGACACCTTG+TGG 0.505367 4.2:+54454700 None:intergenic
GCCGTTCTTCCATGAAAGAT+GGG 0.505531 4.2:+54456065 None:intergenic
CCTTGATTCTGCGGATAAGA+CGG 0.507484 4.2:+54455586 None:intergenic
TCTAACCAATTGCTGAATCA+TGG 0.509733 4.2:-54455260 MS.gene027467:CDS
CACGCTGCAGAACTCACTGC+TGG 0.509974 4.2:-54455055 MS.gene027467:CDS
CAAATTGCAGGATTACTGTT+CGG 0.510588 4.2:-54454492 MS.gene027467:intron
GAGTGCTGGCCATTCTGAAT+GGG 0.513626 4.2:-54455393 MS.gene027467:CDS
CTCGTGCAACTACGAGCAGC+AGG 0.514137 4.2:-54456377 MS.gene027467:CDS
TTCAATGTCTATTATAACCT+CGG 0.515003 4.2:+54455642 None:intergenic
ATATTCCTGGTTCCTAATAC+CGG 0.516943 4.2:+54456112 None:intergenic
TGTTATGATCGATGTCTGGT+GGG 0.519735 4.2:-54456345 MS.gene027467:CDS
CCGATGTCAAGCACCCACTT+TGG 0.522352 4.2:+54456170 None:intergenic
ATCAATGTGGAGGAAATGTA+GGG 0.527842 4.2:-54456214 MS.gene027467:CDS
ATCTTCTACACCAATCGCTC+CGG 0.528546 4.2:-54456131 MS.gene027467:CDS
GGTATCCATCTCTATTATCA+AGG 0.529551 4.2:+54455023 None:intergenic
CATTGAACAAAAGGGACCTA+AGG 0.530418 4.2:-54456318 MS.gene027467:CDS
CTTGATTCTGCGGATAAGAC+GGG 0.531810 4.2:+54455587 None:intergenic
GACCCTGAAAAGTACAATCA+TGG 0.532554 4.2:-54454470 MS.gene027467:CDS
GTTCCTAATACCGGAGCGAT+TGG 0.532915 4.2:+54456121 None:intergenic
GCACATACCTTACTGAGAAA+GGG 0.534073 4.2:-54455304 MS.gene027467:CDS
GTTGTTCTTTAAGTCCATCT+GGG 0.534198 4.2:+54456399 None:intergenic
GCCAATACCGCCGTTCCCCT+GGG 0.540968 4.2:-54454384 MS.gene027467:CDS
ATACCACCGTTAAAGCGATC+AGG 0.546653 4.2:-54454446 MS.gene027467:CDS
ATGTCTGTTTCTGAATCCCA+GGG 0.546684 4.2:+54454368 None:intergenic
TAAAGGTCTCTGGAATTCAC+TGG 0.547752 4.2:-54455096 MS.gene027467:intron
TACAACCTTGATAATAGAGA+TGG 0.550533 4.2:-54455028 MS.gene027467:CDS
AACCAGGAATATCTGAGCAT+TGG 0.551269 4.2:-54456101 MS.gene027467:CDS
CTACGAGCAGCAGGCGTCGA+CGG 0.551976 4.2:-54456368 MS.gene027467:CDS
GTGTTATGATCGATGTCTGG+TGG 0.555121 4.2:-54456346 MS.gene027467:CDS
GTTTCTGAATCCCAGGGGAA+CGG 0.561610 4.2:+54454374 None:intergenic
ACGGTGTTATGATCGATGTC+TGG 0.564978 4.2:-54456349 MS.gene027467:CDS
GTTATGATCGATGTCTGGTG+GGG 0.566303 4.2:-54456344 MS.gene027467:CDS
AACAGGTACTGAGTGGAGGT+TGG 0.567639 4.2:-54454807 MS.gene027467:intron
GAAGTCGGTCTTGGTCCAGC+AGG 0.569062 4.2:-54455622 MS.gene027467:CDS
TCGCTCCGGTATTAGGAACC+AGG 0.580310 4.2:-54456117 MS.gene027467:CDS
AATTGCCATCCTTGATTCTG+CGG 0.582906 4.2:+54455577 None:intergenic
CTGGTAGTTCCCATTCAGAA+TGG 0.585248 4.2:+54455384 None:intergenic
GGGTATCTGAGTTCTCCTGC+TGG 0.587322 4.2:+54455607 None:intergenic
GAAGCTAATAAAGCTTTCCT+GGG 0.588794 4.2:-54455221 MS.gene027467:CDS
AAGACGGCTCTCCAAAACTT+CGG 0.590600 4.2:-54454684 MS.gene027467:CDS
TCATGGAAGAACGGCCATTG+AGG 0.592941 4.2:-54456057 MS.gene027467:intron
GTTATAATAGACATTGAAGT+CGG 0.595729 4.2:-54455637 MS.gene027467:CDS
TAACATACCATTACCAAAGT+GGG 0.596649 4.2:-54456183 MS.gene027467:CDS
ACCCATCTTTCATGGAAGAA+CGG 0.599054 4.2:-54456066 MS.gene027467:CDS
ACCTGCTGAACAAGTTTCTG+TGG 0.599130 4.2:+54454902 None:intergenic
TTATGATCGATGTCTGGTGG+GGG 0.599720 4.2:-54456343 MS.gene027467:CDS
CCAAAGTGGGTGCTTGACAT+CGG 0.611618 4.2:-54456170 MS.gene027467:CDS
ATGTCATTCCATCAATGTGG+AGG 0.631344 4.2:-54456224 MS.gene027467:CDS
AATGTCTTCGAAGACCCAGA+TGG 0.637363 4.2:-54456413 MS.gene027467:CDS
CCACAAGGTGTCAACAAAGA+CGG 0.639776 4.2:-54454700 MS.gene027467:CDS
TTAACATACCATTACCAAAG+TGG 0.641878 4.2:-54456184 MS.gene027467:CDS
CTTATCCGCAGAATCAAGGA+TGG 0.644913 4.2:-54455582 MS.gene027467:CDS
TCTGAATCCCAGGGGAACGG+CGG 0.648038 4.2:+54454377 None:intergenic
ATACTGAATGCAAGACCACA+AGG 0.668768 4.2:-54454715 MS.gene027467:CDS
AGGTCTCTGGAATTCACTGG+TGG 0.672373 4.2:-54455093 MS.gene027467:CDS
ATCATGTCATTCCATCAATG+TGG 0.675140 4.2:-54456227 MS.gene027467:CDS
TTAGATCATCTGATAGACGA+AGG 0.691757 4.2:+54454644 None:intergenic
TGGGAACTACCAGATGACGC+TGG 0.712299 4.2:-54455374 MS.gene027467:CDS
TCAATGTGGAGGAAATGTAG+GGG 0.715859 4.2:-54456213 MS.gene027467:CDS
TATGAACAGGTACTGAGTGG+AGG 0.727187 4.2:-54454811 MS.gene027467:intron
TGTCTGTTTCTGAATCCCAG+GGG 0.737797 4.2:+54454369 None:intergenic
ATGTATGAACAGGTACTGAG+TGG 0.748627 4.2:-54454814 MS.gene027467:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTTAGAGGTTAATTATT+TGG + chr4.2:54455820-54455839 None:intergenic 20.0%
!! ATTATTTGGAAGAAAAATGA+AGG + chr4.2:54455806-54455825 None:intergenic 20.0%
!! TGATATATTCAAAAAGTTTG+TGG - chr4.2:54456337-54456356 MS.gene027467:CDS 20.0%
!!! AAGTTTATTTAGAAGTACTA+TGG + chr4.2:54455211-54455230 None:intergenic 20.0%
! AACTAATATCCATACTGATA+TGG - chr4.2:54454944-54454963 MS.gene027467:CDS 25.0%
! CCTCTAACTATGTATAATAA+AGG - chr4.2:54455829-54455848 MS.gene027467:intron 25.0%
! CCTTTATTATACATAGTTAG+AGG + chr4.2:54455832-54455851 None:intergenic 25.0%
! GTGTTATGACAAATATTTGA+AGG - chr4.2:54455504-54455523 MS.gene027467:intron 25.0%
! GTTATAATAGACATTGAAGT+CGG - chr4.2:54455305-54455324 MS.gene027467:CDS 25.0%
! TAAATTGTGTGAAACTATCT+GGG - chr4.2:54455151-54455170 MS.gene027467:intron 25.0%
! TTAAATTGTGTGAAACTATC+TGG - chr4.2:54455150-54455169 MS.gene027467:intron 25.0%
! TTCAATGTCTATTATAACCT+CGG + chr4.2:54455303-54455322 None:intergenic 25.0%
!! TTTCAACTTGTTCAAAAATG+TGG - chr4.2:54454676-54454695 MS.gene027467:CDS 25.0%
AAAATGTGAAGTGCATTACA+TGG + chr4.2:54456394-54456413 None:intergenic 30.0%
AAGGTGATATTCGTTAATTG+CGG + chr4.2:54455787-54455806 None:intergenic 30.0%
AATTTAACATGTAGAGCAAG+CGG + chr4.2:54456097-54456116 None:intergenic 30.0%
ACCGAATTCTTCAAAACAAA+CGG - chr4.2:54455616-54455635 MS.gene027467:CDS 30.0%
ATTGGTTAGAATACCAAGTT+AGG + chr4.2:54455672-54455691 None:intergenic 30.0%
CCATGTAAGAGAATTAACTA+GGG - chr4.2:54454975-54454994 MS.gene027467:CDS 30.0%
CTATGTATAATAAAGGTCTC+TGG - chr4.2:54455836-54455855 MS.gene027467:intron 30.0%
TAACATACCATTACCAAAGT+GGG - chr4.2:54454759-54454778 MS.gene027467:CDS 30.0%
TCTCAACCAATGATATAGAT+CGG + chr4.2:54455064-54455083 None:intergenic 30.0%
TTAACATACCATTACCAAAG+TGG - chr4.2:54454758-54454777 MS.gene027467:CDS 30.0%
TTTGGTTACTGGAATAAGAT+AGG - chr4.2:54454999-54455018 MS.gene027467:CDS 30.0%
! AAGAGAATTAACTAGGGTTT+TGG - chr4.2:54454981-54455000 MS.gene027467:CDS 30.0%
! ATTTTACATGTCTTGAGATG+AGG - chr4.2:54455984-54456003 MS.gene027467:intron 30.0%
! TACAACCTTGATAATAGAGA+TGG - chr4.2:54455914-54455933 MS.gene027467:intron 30.0%
! TTTTACATGTCTTGAGATGA+GGG - chr4.2:54455985-54456004 MS.gene027467:intron 30.0%
!! AAGCTTTATTAGCTTCATCT+AGG + chr4.2:54455717-54455736 None:intergenic 30.0%
!! AGAAGTACTATGGTAACTAA+TGG + chr4.2:54455201-54455220 None:intergenic 30.0%
!! CTTAGTCATTGATTTGAGAT+CGG + chr4.2:54455127-54455146 None:intergenic 30.0%
AAAATTAATGCATCCGATCC+GGG - chr4.2:54455174-54455193 MS.gene027467:intron 35.0%
AACAGTAATCCTGCAATTTG+AGG + chr4.2:54456450-54456469 None:intergenic 35.0%
AACCAATGATATAGATCGGA+AGG + chr4.2:54455060-54455079 None:intergenic 35.0%
AAGGGAAATTCTTCCTAACT+TGG - chr4.2:54455656-54455675 MS.gene027467:CDS 35.0%
ACAGTAATCCTGCAATTTGA+GGG + chr4.2:54456449-54456468 None:intergenic 35.0%
ACTGTATATCTGTGCAAAGT+AGG + chr4.2:54455234-54455253 None:intergenic 35.0%
AGAACATCATCAGGAATCTT+TGG + chr4.2:54456523-54456542 None:intergenic 35.0%
AGAATTTCCCTTTCTCAGTA+AGG + chr4.2:54455648-54455667 None:intergenic 35.0%
AGAGAAAACATTGAAGTTGC+TGG - chr4.2:54456158-54456177 MS.gene027467:CDS 35.0%
AGTTGTTCTTTAAGTCCATC+TGG + chr4.2:54454547-54454566 None:intergenic 35.0%
ATATTCCTGGTTCCTAATAC+CGG + chr4.2:54454833-54454852 None:intergenic 35.0%
ATCAATGTGGAGGAAATGTA+GGG - chr4.2:54454728-54454747 MS.gene027467:CDS 35.0%
ATCATGTCATTCCATCAATG+TGG - chr4.2:54454715-54454734 MS.gene027467:CDS 35.0%
ATCCTTCCGATCTATATCAT+TGG - chr4.2:54455055-54455074 MS.gene027467:CDS 35.0%
CAAAGTTTAACTCACCTCAA+TGG + chr4.2:54454902-54454921 None:intergenic 35.0%
CAAATTGCAGGATTACTGTT+CGG - chr4.2:54456450-54456469 MS.gene027467:intron 35.0%
CACAGTAGTTTATGTGTATG+AGG + chr4.2:54454421-54454440 None:intergenic 35.0%
CCCTAGTTAATTCTCTTACA+TGG + chr4.2:54454978-54454997 None:intergenic 35.0%
CGAAACATGAATGTATGAAC+AGG - chr4.2:54456118-54456137 MS.gene027467:CDS 35.0%
GAAAATTAATGCATCCGATC+CGG - chr4.2:54455173-54455192 MS.gene027467:intron 35.0%
GAAACAGACATGAAAGTTGA+TGG - chr4.2:54456586-54456605 MS.gene027467:CDS 35.0%
GAAGCTAATAAAGCTTTCCT+GGG - chr4.2:54455721-54455740 MS.gene027467:intron 35.0%
GAAGGATGAGATCAATTGTT+GGG + chr4.2:54455042-54455061 None:intergenic 35.0%
GATAACAAACCCATCTTTCA+TGG - chr4.2:54454868-54454887 MS.gene027467:intron 35.0%
GCCATGTAAGAGAATTAACT+AGG - chr4.2:54454974-54454993 MS.gene027467:CDS 35.0%
GCTTCATTAAGAACATCATC+AGG + chr4.2:54456532-54456551 None:intergenic 35.0%
GCTTCATTTCCATATCAGTA+TGG + chr4.2:54454956-54454975 None:intergenic 35.0%
GGTATCCATCTCTATTATCA+AGG + chr4.2:54455922-54455941 None:intergenic 35.0%
GTCTTGCATTCAGTATGATT+TGG + chr4.2:54456223-54456242 None:intergenic 35.0%
GTTGTTCTTTAAGTCCATCT+GGG + chr4.2:54454546-54454565 None:intergenic 35.0%
TATGTGTATGAGGACTTACT+GGG + chr4.2:54454411-54454430 None:intergenic 35.0%
TCTAACCAATTGCTGAATCA+TGG - chr4.2:54455682-54455701 MS.gene027467:CDS 35.0%
TGAAGCTAATAAAGCTTTCC+TGG - chr4.2:54455720-54455739 MS.gene027467:intron 35.0%
TTAGATCATCTGATAGACGA+AGG + chr4.2:54456301-54456320 None:intergenic 35.0%
TTATGTGTATGAGGACTTAC+TGG + chr4.2:54454412-54454431 None:intergenic 35.0%
TTGAAGAATTCGGTAGATTC+TGG + chr4.2:54455610-54455629 None:intergenic 35.0%
! ATGCCATGATTGTACTTTTC+AGG + chr4.2:54456478-54456497 None:intergenic 35.0%
! CTAATTTGACTTTACATCCC+AGG + chr4.2:54455741-54455760 None:intergenic 35.0%
! TGCCATGATTGTACTTTTCA+GGG + chr4.2:54456477-54456496 None:intergenic 35.0%
!! CATTGTTTTTCTTCGTAGCT+CGG - chr4.2:54454490-54454509 MS.gene027467:intron 35.0%
!!! ACAGTTTTGATTGAAAGCTG+AGG + chr4.2:54455423-54455442 None:intergenic 35.0%
!!! ACGAGAGACTATTTTTGCAA+TGG + chr4.2:54455948-54455967 None:intergenic 35.0%
!!! AGACTATTTTTGCAATGGGA+CGG + chr4.2:54455943-54455962 None:intergenic 35.0%
!!! CGAGAGACTATTTTTGCAAT+GGG + chr4.2:54455947-54455966 None:intergenic 35.0%
!!! GCCGTTTGTTTTGAAGAATT+CGG + chr4.2:54455620-54455639 None:intergenic 35.0%
!!! GTCAGATCTTTTAAAATCCG+AGG - chr4.2:54455283-54455302 MS.gene027467:CDS 35.0%
!!! TTAACTAGGGTTTTGGTTAC+TGG - chr4.2:54454988-54455007 MS.gene027467:CDS 35.0%
AACCAGGAATATCTGAGCAT+TGG - chr4.2:54454841-54454860 MS.gene027467:intron 40.0%
AATCAAGGATGGCAATTTCC+TGG - chr4.2:54455371-54455390 MS.gene027467:CDS 40.0%
AATTGCCATCCTTGATTCTG+CGG + chr4.2:54455368-54455387 None:intergenic 40.0%
AATTTGAGGGAAGAGGGTTA+AGG + chr4.2:54456436-54456455 None:intergenic 40.0%
ACCCATCTTTCATGGAAGAA+CGG - chr4.2:54454876-54454895 MS.gene027467:intron 40.0%
ATACTGAATGCAAGACCACA+AGG - chr4.2:54456227-54456246 MS.gene027467:CDS 40.0%
ATAGACATTGAAGTCGGTCT+TGG - chr4.2:54455311-54455330 MS.gene027467:CDS 40.0%
ATGTCATTCCATCAATGTGG+AGG - chr4.2:54454718-54454737 MS.gene027467:CDS 40.0%
ATTTGAGGGAAGAGGGTTAA+GGG + chr4.2:54456435-54456454 None:intergenic 40.0%
CATCAATGTGGAGGAAATGT+AGG - chr4.2:54454727-54454746 MS.gene027467:CDS 40.0%
CATTGAACAAAAGGGACCTA+AGG - chr4.2:54454624-54454643 MS.gene027467:CDS 40.0%
CGATTGGTGTAGAAGATATC+AGG + chr4.2:54454808-54454827 None:intergenic 40.0%
CTACATTTCCTCCACATTGA+TGG + chr4.2:54454729-54454748 None:intergenic 40.0%
CTGGAGAAAATGCACTTTCA+AGG - chr4.2:54456177-54456196 MS.gene027467:CDS 40.0%
GATCACCATGATTCAGCAAT+TGG + chr4.2:54455690-54455709 None:intergenic 40.0%
GCACATACCTTACTGAGAAA+GGG - chr4.2:54455638-54455657 MS.gene027467:CDS 40.0%
GGAAGGATGAGATCAATTGT+TGG + chr4.2:54455043-54455062 None:intergenic 40.0%
TCAATGTGGAGGAAATGTAG+GGG - chr4.2:54454729-54454748 MS.gene027467:CDS 40.0%
TCTCCAAAACTTCGGATGTA+TGG - chr4.2:54456266-54456285 MS.gene027467:CDS 40.0%
TGGGGGATCATTGAACAAAA+GGG - chr4.2:54454616-54454635 MS.gene027467:CDS 40.0%
TGTTATGATCGATGTCTGGT+GGG - chr4.2:54454597-54454616 MS.gene027467:CDS 40.0%
TTTGGTCCTGATCGCTTTAA+CGG + chr4.2:54456505-54456524 None:intergenic 40.0%
! ACTATGGTAACTAATGGACC+CGG + chr4.2:54455195-54455214 None:intergenic 40.0%
! ACTCCATACATCCGAAGTTT+TGG + chr4.2:54456272-54456291 None:intergenic 40.0%
! GACCCTGAAAAGTACAATCA+TGG - chr4.2:54456472-54456491 MS.gene027467:intron 40.0%
!! ATGTATGAACAGGTACTGAG+TGG - chr4.2:54456128-54456147 MS.gene027467:CDS 40.0%
!! ATGTCTGTTTCTGAATCCCA+GGG + chr4.2:54456577-54456596 None:intergenic 40.0%
!! TAAAGGTCTCTGGAATTCAC+TGG - chr4.2:54455846-54455865 MS.gene027467:intron 40.0%
AAGACGGCTCTCCAAAACTT+CGG - chr4.2:54456258-54456277 MS.gene027467:CDS 45.0%
AATGTCTTCGAAGACCCAGA+TGG - chr4.2:54454529-54454548 MS.gene027467:intron 45.0%
ACCTGAAACTCTCCAATTCC+AGG + chr4.2:54455392-54455411 None:intergenic 45.0%
ACCTGCTGAACAAGTTTCTG+TGG + chr4.2:54456043-54456062 None:intergenic 45.0%
ATACCACCGTTAAAGCGATC+AGG - chr4.2:54456496-54456515 MS.gene027467:intron 45.0%
ATCCTGCAATTTGAGGGAAG+AGG + chr4.2:54456443-54456462 None:intergenic 45.0%
ATCTTCTACACCAATCGCTC+CGG - chr4.2:54454811-54454830 MS.gene027467:intron 45.0%
CACCAATGCTCAGATATTCC+TGG + chr4.2:54454846-54454865 None:intergenic 45.0%
CCTTGATTCTGCGGATAAGA+CGG + chr4.2:54455359-54455378 None:intergenic 45.0%
CTGGTAGTTCCCATTCAGAA+TGG + chr4.2:54455561-54455580 None:intergenic 45.0%
CTTATCCGCAGAATCAAGGA+TGG - chr4.2:54455360-54455379 MS.gene027467:CDS 45.0%
CTTGATTCTGCGGATAAGAC+GGG + chr4.2:54455358-54455377 None:intergenic 45.0%
GCACTCCAATCATACTCCTT+AGG + chr4.2:54454643-54454662 None:intergenic 45.0%
GCCGTTCTTCCATGAAAGAT+GGG + chr4.2:54454880-54454899 None:intergenic 45.0%
GGATGGCAATTTCCTGGAAT+TGG - chr4.2:54455377-54455396 MS.gene027467:CDS 45.0%
GGCACATACCTTACTGAGAA+AGG - chr4.2:54455637-54455656 MS.gene027467:CDS 45.0%
GGTAAAGATGCATGCAGATC+AGG - chr4.2:54456358-54456377 MS.gene027467:CDS 45.0%
GTGGGGGATCATTGAACAAA+AGG - chr4.2:54454615-54454634 MS.gene027467:CDS 45.0%
GTGTTATGATCGATGTCTGG+TGG - chr4.2:54454596-54454615 MS.gene027467:CDS 45.0%
GTTATGATCGATGTCTGGTG+GGG - chr4.2:54454598-54454617 MS.gene027467:CDS 45.0%
TCAAGCACCCACTTTGGTAA+TGG + chr4.2:54454769-54454788 None:intergenic 45.0%
TCCTGCAATTTGAGGGAAGA+GGG + chr4.2:54456442-54456461 None:intergenic 45.0%
TCCTGGAATTGGAGAGTTTC+AGG - chr4.2:54455388-54455407 MS.gene027467:CDS 45.0%
TTATGATCGATGTCTGGTGG+GGG - chr4.2:54454599-54454618 MS.gene027467:CDS 45.0%
TTTGAGGGAAGAGGGTTAAG+GGG + chr4.2:54456434-54456453 None:intergenic 45.0%
! ACCACAGAAACTTGTTCAGC+AGG - chr4.2:54456039-54456058 MS.gene027467:intron 45.0%
! ACTGGGAGCATGACATAAAC+TGG + chr4.2:54454394-54454413 None:intergenic 45.0%
! AGGGACCTAAGGAGTATGAT+TGG - chr4.2:54454635-54454654 MS.gene027467:CDS 45.0%
! CCACAAGGTGTCAACAAAGA+CGG - chr4.2:54456242-54456261 MS.gene027467:CDS 45.0%
!! ACGGTGTTATGATCGATGTC+TGG - chr4.2:54454593-54454612 MS.gene027467:CDS 45.0%
!! CATGTCTGTTTCTGAATCCC+AGG + chr4.2:54456578-54456597 None:intergenic 45.0%
!! TATGAACAGGTACTGAGTGG+AGG - chr4.2:54456131-54456150 MS.gene027467:CDS 45.0%
!! TGTCTGTTTCTGAATCCCAG+GGG + chr4.2:54456576-54456595 None:intergenic 45.0%
ACACCAATCGCTCCGGTATT+AGG - chr4.2:54454818-54454837 MS.gene027467:intron 50.0%
ACCCTCTTCCCTCAAATTGC+AGG - chr4.2:54456438-54456457 MS.gene027467:intron 50.0%
CCAAAGTGGGTGCTTGACAT+CGG - chr4.2:54454772-54454791 MS.gene027467:CDS 50.0%
CCGTCTTATCCGCAGAATCA+AGG - chr4.2:54455356-54455375 MS.gene027467:CDS 50.0%
CCGTCTTTGTTGACACCTTG+TGG + chr4.2:54456245-54456264 None:intergenic 50.0%
GGCCGTTCTTCCATGAAAGA+TGG + chr4.2:54454881-54454900 None:intergenic 50.0%
GTGAGTTCTGCAGCGTGATT+CGG + chr4.2:54455884-54455903 None:intergenic 50.0%
GTTCCTAATACCGGAGCGAT+TGG + chr4.2:54454824-54454843 None:intergenic 50.0%
GTTTCTGAATCCCAGGGGAA+CGG + chr4.2:54456571-54456590 None:intergenic 50.0%
TCATGGAAGAACGGCCATTG+AGG - chr4.2:54454885-54454904 MS.gene027467:intron 50.0%
! GGTAACTAATGGACCCGGAT+CGG + chr4.2:54455190-54455209 None:intergenic 50.0%
!! AACAGGTACTGAGTGGAGGT+TGG - chr4.2:54456135-54456154 MS.gene027467:CDS 50.0%
!! AGGTCTCTGGAATTCACTGG+TGG - chr4.2:54455849-54455868 MS.gene027467:intron 50.0%
!! GAGTGCTGGCCATTCTGAAT+GGG - chr4.2:54455549-54455568 MS.gene027467:intron 50.0%
CCGATGTCAAGCACCCACTT+TGG + chr4.2:54454775-54454794 None:intergenic 55.0%
GGGTATCTGAGTTCTCCTGC+TGG + chr4.2:54455338-54455357 None:intergenic 55.0%
GGTCCTGATCGCTTTAACGG+TGG + chr4.2:54456502-54456521 None:intergenic 55.0%
TCGCTCCGGTATTAGGAACC+AGG - chr4.2:54454825-54454844 MS.gene027467:intron 55.0%
TGGGAACTACCAGATGACGC+TGG - chr4.2:54455568-54455587 MS.gene027467:CDS 55.0%
TTGTACGTGCCAGCGTCATC+TGG + chr4.2:54455580-54455599 None:intergenic 55.0%
!! CGAGTGCTGGCCATTCTGAA+TGG - chr4.2:54455548-54455567 MS.gene027467:intron 55.0%
AAAGCAGCTGCAGCGAGTGC+TGG - chr4.2:54455535-54455554 MS.gene027467:intron 60.0%
CACGCTGCAGAACTCACTGC+TGG - chr4.2:54455887-54455906 MS.gene027467:intron 60.0%
CTCGTGCAACTACGAGCAGC+AGG - chr4.2:54454565-54454584 MS.gene027467:intron 60.0%
GAAGTCGGTCTTGGTCCAGC+AGG - chr4.2:54455320-54455339 MS.gene027467:CDS 60.0%
TCTGAATCCCAGGGGAACGG+CGG + chr4.2:54456568-54456587 None:intergenic 60.0%
AGCCAATACCGCCGTTCCCC+TGG - chr4.2:54456557-54456576 MS.gene027467:CDS 65.0%
GCCAATACCGCCGTTCCCCT+GGG - chr4.2:54456558-54456577 MS.gene027467:CDS 65.0%
TCCCAGGGGAACGGCGGTAT+TGG + chr4.2:54456562-54456581 None:intergenic 65.0%
!! CTACGAGCAGCAGGCGTCGA+CGG - chr4.2:54454574-54454593 MS.gene027467:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 54454352 54456612 54454352 ID=MS.gene027467
chr4.2 mRNA 54454352 54456612 54454352 ID=MS.gene027467.t1;Parent=MS.gene027467
chr4.2 exon 54456553 54456612 54456553 ID=MS.gene027467.t1.exon1;Parent=MS.gene027467.t1
chr4.2 CDS 54456553 54456612 54456553 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
chr4.2 exon 54456058 54456456 54456058 ID=MS.gene027467.t1.exon2;Parent=MS.gene027467.t1
chr4.2 CDS 54456058 54456456 54456058 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
chr4.2 exon 54455555 54455719 54455555 ID=MS.gene027467.t1.exon3;Parent=MS.gene027467.t1
chr4.2 CDS 54455555 54455719 54455555 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
chr4.2 exon 54455196 54455459 54455196 ID=MS.gene027467.t1.exon4;Parent=MS.gene027467.t1
chr4.2 CDS 54455196 54455459 54455196 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
chr4.2 exon 54454904 54455113 54454904 ID=MS.gene027467.t1.exon5;Parent=MS.gene027467.t1
chr4.2 CDS 54454904 54455113 54454904 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
chr4.2 exon 54454585 54454824 54454585 ID=MS.gene027467.t1.exon6;Parent=MS.gene027467.t1
chr4.2 CDS 54454585 54454824 54454585 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
chr4.2 exon 54454352 54454504 54454352 ID=MS.gene027467.t1.exon7;Parent=MS.gene027467.t1
chr4.2 CDS 54454352 54454504 54454352 ID=cds.MS.gene027467.t1;Parent=MS.gene027467.t1
Gene Sequence

>MS.gene027467

ATGTCTACTTTGAACAAAAACATGCTTCTAAATTATGTTCCAGTTTATGTCATGCTCCCAGTAAGTCCTCATACACATAAACTACTGTGTTTGTAAGCTCATAAATATGTGTTTGTTCACTAATATTAACATATATTTCATTGTTTTTCTTCGTAGCTCGGAGTTATTAATGTCAATAATGTCTTCGAAGACCCAGATGGACTTAAAGAACAACTCGTGCAACTACGAGCAGCAGGCGTCGACGGTGTTATGATCGATGTCTGGTGGGGGATCATTGAACAAAAGGGACCTAAGGAGTATGATTGGAGTGCTTATAAGAGCTTGTTTCAACTTGTTCAAAAATGTGGCTTGAAATTGCAAGCTATCATGTCATTCCATCAATGTGGAGGAAATGTAGGGGATGTTGTTAACATACCATTACCAAAGTGGGTGCTTGACATCGGAGAATCAGATCCTGATATCTTCTACACCAATCGCTCCGGTATTAGGAACCAGGAATATCTGAGCATTGGTGTAGATAACAAACCCATCTTTCATGGAAGAACGGCCATTGAGGTGAGTTAAACTTTGAAATTGTAATTATCTTTCTTTTAACTAATATCCATACTGATATGGAAATGAAGCCATGTAAGAGAATTAACTAGGGTTTTGGTTACTGGAATAAGATAGGCTGCAGTTACTGCGTCACCCAACAATTGATCTCATCCTTCCGATCTATATCATTGGTTGAGATCGATTAAAATTAATTTAATGAATGTATAATCTGAGTTGTCCGATCTCAAATCAATGACTAAGATTTTAAATTGTGTGAAACTATCTGGGAAAATTAATGCATCCGATCCGGGTCCATTAGTTACCATAGTACTTCTAAATAAACTTCCTACTTTGCACAGATATACAGTGATTACATGAAGAGTTTCAGAGAAAACATGTCAGATCTTTTAAAATCCGAGGTTATAATAGACATTGAAGTCGGTCTTGGTCCAGCAGGAGAACTCAGATACCCGTCTTATCCGCAGAATCAAGGATGGCAATTTCCTGGAATTGGAGAGTTTCAGGTGATAATTGCCTCAGCTTTCAATCAAAACTGTTTCTTACACATACTGCAATGTCATCTCAGCACTAAAATTTTCATAAAAATGAAATTATGCAGTGTTATGACAAATATTTGAAGGAAAGTTTCAAAGCAGCTGCAGCGAGTGCTGGCCATTCTGAATGGGAACTACCAGATGACGCTGGCACGTACAATGACGTACCAGAATCTACCGAATTCTTCAAAACAAACGGCACATACCTTACTGAGAAAGGGAAATTCTTCCTAACTTGGTATTCTAACCAATTGCTGAATCATGGTGATCAAATCCTAGATGAAGCTAATAAAGCTTTCCTGGGATGTAAAGTCAAATTAGCAATCAAAGTAAAATTCTTAACACCGCAATTAACGAATATCACCTTCATTTTTCTTCCAAATAATTAACCTCTAACTATGTATAATAAAGGTCTCTGGAATTCACTGGTGGTATAAAGCTCCGAATCACGCTGCAGAACTCACTGCTGGATATTACAACCTTGATAATAGAGATGGATACCGTCCCATTGCAAAAATAGTCTCTCGTCATCATGCTATTTTGAATTTTACATGTCTTGAGATGAGGGACTCAGAACAAAGCTCTGATGCACATAGTTCACCACAGAAACTTGTTCAGCAGGTAAATCATTACAATTTTCTTTAGTTTCATGCACCGCTTGCTCTACATGTTAAATTGCGAAACATGAATGTATGAACAGGTACTGAGTGGAGGTTGGAGAGAAAACATTGAAGTTGCTGGAGAAAATGCACTTTCAAGGTATGATGCAACAGCTTATAACCAAATCATACTGAATGCAAGACCACAAGGTGTCAACAAAGACGGCTCTCCAAAACTTCGGATGTATGGAGTAACATACCTTCGTCTATCAGATGATCTAATGCAACAATCAAATTTTGATATATTCAAAAAGTTTGTGGTAAAGATGCATGCAGATCAGGTGAGCATATGCCATGTAATGCACTTCACATTTTTCATAATCATCTTCAAGCCCCTTAACCCTCTTCCCTCAAATTGCAGGATTACTGTTCGGACCCTGAAAAGTACAATCATGGCATACCACCGTTAAAGCGATCAGGACCAAAGATTCCTGATGATGTTCTTAATGAAGCAACTAAGCCAATACCGCCGTTCCCCTGGGATTCAGAAACAGACATGAAAGTTGATGGTTGA
Protein sequence

>MS.gene027467.t1

MSTLNKNMLLNYVPVYVMLPLGVINVNNVFEDPDGLKEQLVQLRAAGVDGVMIDVWWGIIEQKGPKEYDWSAYKSLFQLVQKCGLKLQAIMSFHQCGGNVGDVVNIPLPKWVLDIGESDPDIFYTNRSGIRNQEYLSIGVDNKPIFHGRTAIEIYSDYMKSFRENMSDLLKSEVIIDIEVGLGPAGELRYPSYPQNQGWQFPGIGEFQCYDKYLKESFKAAAASAGHSEWELPDDAGTYNDVPESTEFFKTNGTYLTEKGKFFLTWYSNQLLNHGDQILDEANKAFLGCKVKLAIKVSGIHWWYKAPNHAAELTAGYYNLDNRDGYRPIAKIVSRHHAILNFTCLEMRDSEQSSDAHSSPQKLVQQVLSGGWRENIEVAGENALSRYDATAYNQIILNARPQGVNKDGSPKLRMYGVTYLRLSDDLMQQSNFDIFKKFVVKMHADQDYCSDPEKYNHGIPPLKRSGPKIPDDVLNEATKPIPPFPWDSETDMKVDG