AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027565

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027565.t1 MTR_5g035150 95.172 435 21 0 1 435 1 435 0.0 847
MS.gene027565.t1 MTR_5g035070 64.828 435 151 2 1 435 5 437 0.0 562
MS.gene027565.t1 MTR_5g035090 62.069 435 159 3 1 435 3 431 0.0 546
MS.gene027565.t1 MTR_5g035080 65.926 405 118 5 34 435 37 424 0.0 540
MS.gene027565.t1 MTR_3g109840 61.239 436 142 8 1 433 6 417 0.0 526
MS.gene027565.t1 MTR_8g104970 56.710 462 150 9 16 432 10 466 0.0 514
MS.gene027565.t1 MTR_7g033230 59.626 374 138 5 69 433 242 611 9.88e-161 467
MS.gene027565.t1 MTR_7g033230 59.626 374 138 5 69 433 312 681 9.97e-160 467
MS.gene027565.t1 MTR_5g035140 64.906 265 86 4 172 433 7 267 4.67e-126 366
MS.gene027565.t1 MTR_8g099300 41.761 352 194 7 85 431 74 419 1.55e-95 293
MS.gene027565.t1 MTR_8g027900 40.227 353 201 5 83 431 209 555 1.67e-94 295
MS.gene027565.t1 MTR_6g016880 37.590 415 241 10 25 432 17 420 1.21e-93 289
MS.gene027565.t1 MTR_7g019120 35.593 413 244 10 29 432 22 421 5.47e-88 275
MS.gene027565.t1 MTR_6g016875 38.814 371 213 7 68 432 47 409 8.09e-84 263
MS.gene027565.t1 MTR_6g007777 38.202 356 207 7 82 433 113 459 2.69e-83 263
MS.gene027565.t1 MTR_2g016060 37.640 356 197 12 90 433 60 402 2.59e-75 241
MS.gene027565.t1 MTR_6g061710 33.069 378 200 13 83 433 165 516 4.56e-64 215
MS.gene027565.t1 MTR_1g076690 33.333 363 215 11 85 433 223 572 4.57e-64 216
MS.gene027565.t1 MTR_7g094750 32.880 368 206 14 84 433 90 434 3.01e-53 184
MS.gene027565.t1 MTR_2g022560 33.693 371 202 14 84 435 96 441 2.85e-49 174
MS.gene027565.t1 MTR_5g035120 49.419 172 76 2 4 175 5 165 6.59e-48 162
MS.gene027565.t1 MTR_4g126950 32.418 364 199 14 83 427 107 442 6.90e-47 167
MS.gene027565.t1 MTR_0136s0010 32.970 367 204 15 85 433 170 512 8.12e-47 169
MS.gene027565.t1 MTR_7g093450 31.343 402 239 16 43 427 202 583 1.00e-45 167
MS.gene027565.t1 MTR_7g109550 31.844 358 198 14 85 427 137 463 7.55e-44 160
MS.gene027565.t1 MTR_5g059400 31.129 363 206 15 84 427 174 511 8.10e-44 160
MS.gene027565.t1 MTR_1g116130 31.129 363 206 15 84 427 174 511 8.10e-44 160
MS.gene027565.t1 MTR_4g014440 31.111 360 213 16 85 427 62 403 2.61e-42 154
MS.gene027565.t1 MTR_1g058380 28.804 368 217 13 83 431 53 394 2.83e-42 154
MS.gene027565.t1 MTR_4g125060 29.428 367 221 12 76 427 124 467 3.65e-42 155
MS.gene027565.t1 MTR_4g016940 29.491 373 219 16 79 431 79 427 8.40e-42 153
MS.gene027565.t1 MTR_4g125060 29.428 367 221 12 76 427 163 506 8.98e-42 155
MS.gene027565.t1 MTR_4g125060 29.428 367 221 12 76 427 192 535 1.07e-41 155
MS.gene027565.t1 MTR_4g121560 27.830 424 253 17 27 431 64 453 1.12e-41 154
MS.gene027565.t1 MTR_8g093530 30.328 366 217 12 75 431 80 416 1.71e-41 152
MS.gene027565.t1 MTR_8g069790 30.319 376 218 16 73 432 171 518 1.99e-41 154
MS.gene027565.t1 MTR_7g070060 31.044 364 208 16 85 427 64 405 3.87e-41 151
MS.gene027565.t1 MTR_8g463790 27.059 425 259 15 23 427 12 405 5.23e-41 151
MS.gene027565.t1 MTR_5g071350 30.110 362 226 10 77 427 50 395 5.55e-41 150
MS.gene027565.t1 MTR_2g073510 31.831 355 206 13 82 431 30 353 3.34e-40 147
MS.gene027565.t1 MTR_7g068920 31.088 386 214 16 69 431 65 421 6.47e-40 148
MS.gene027565.t1 MTR_3g035830 29.805 359 195 11 83 427 29 344 1.41e-39 145
MS.gene027565.t1 MTR_5g033800 27.717 368 228 11 81 433 141 485 1.45e-39 148
MS.gene027565.t1 MTR_3g021060 30.747 348 206 11 83 427 44 359 3.79e-38 142
MS.gene027565.t1 MTR_3g021090 30.387 362 189 14 83 427 29 344 1.15e-37 142
MS.gene027565.t1 MTR_3g464570 30.086 349 225 11 82 427 40 372 2.14e-37 140
MS.gene027565.t1 MTR_3g070900 27.778 360 229 12 82 427 103 445 2.92e-37 141
MS.gene027565.t1 MTR_3g049330 30.556 360 210 13 75 427 20 346 1.95e-36 137
MS.gene027565.t1 MTR_3g090120 29.189 370 202 12 84 431 128 459 2.21e-36 139
MS.gene027565.t1 MTR_6g016240 28.381 451 258 18 8 431 4 416 2.69e-36 138
MS.gene027565.t1 MTR_2g073500 30.726 358 202 14 82 431 26 345 4.40e-36 136
MS.gene027565.t1 MTR_7g094750 33.992 253 138 9 84 321 90 328 6.85e-36 135
MS.gene027565.t1 MTR_8g093870 26.722 363 231 9 83 431 131 472 1.04e-35 137
MS.gene027565.t1 MTR_4g088030 29.333 375 224 11 55 427 11 346 7.54e-35 132
MS.gene027565.t1 MTR_5g083260 27.901 362 224 12 82 427 80 420 1.03e-34 134
MS.gene027565.t1 MTR_4g092830 28.537 410 227 16 54 431 17 392 1.41e-34 134
MS.gene027565.t1 MTR_2g091175 31.700 347 199 13 85 427 50 362 5.18e-34 130
MS.gene027565.t1 MTR_4g079700 29.921 381 231 13 55 431 15 363 1.00e-33 130
MS.gene027565.t1 MTR_4g087730 30.347 346 208 10 84 427 44 358 1.19e-33 130
MS.gene027565.t1 MTR_1g050448 28.533 368 231 13 67 431 37 375 1.58e-33 129
MS.gene027565.t1 MTR_3g071650 28.056 360 220 11 85 427 89 426 1.64e-33 130
MS.gene027565.t1 MTR_1g112210 27.298 359 216 13 80 427 54 378 1.68e-32 127
MS.gene027565.t1 MTR_2g015720 30.199 351 207 13 85 431 42 358 3.78e-32 125
MS.gene027565.t1 MTR_5g034810 29.513 349 209 12 83 427 33 348 6.13e-32 125
MS.gene027565.t1 MTR_5g082880 28.219 365 222 15 83 427 163 507 2.50e-31 125
MS.gene027565.t1 MTR_4g014430 27.838 370 217 16 83 431 106 446 2.62e-30 122
MS.gene027565.t1 MTR_1g112210 26.124 356 211 12 80 427 54 365 5.54e-30 119
MS.gene027565.t1 MTR_1g111010 28.412 359 208 15 79 431 26 341 5.64e-29 116
MS.gene027565.t1 MTR_7g019140 37.086 151 86 3 29 174 22 168 1.53e-28 110
MS.gene027565.t1 MTR_5g033800 31.667 180 116 3 81 254 141 319 8.60e-27 111
MS.gene027565.t1 MTR_7g070060 29.260 311 178 15 137 427 15 303 3.27e-25 105
MS.gene027565.t1 MTR_7g070060 30.488 246 146 9 85 319 64 295 6.14e-24 101
MS.gene027565.t1 MTR_4g102570 38.583 127 66 3 201 321 2 122 3.90e-22 92.8
MS.gene027565.t1 MTR_3g464570 28.369 282 183 11 149 427 3 268 6.09e-21 92.4
MS.gene027565.t1 MTR_7g117160 31.633 196 81 6 235 430 47 189 2.37e-19 85.9
MS.gene027565.t1 MTR_7g019130 31.200 125 79 3 206 326 4 125 5.31e-13 66.6
MS.gene027565.t1 MTR_0929s0010 31.818 110 66 3 29 133 22 127 1.99e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027565.t1 AT1G70230 49.070 430 193 10 10 435 8 415 1.92e-148 429
MS.gene027565.t1 AT5G15890 40.052 387 213 8 50 432 152 523 2.85e-99 306
MS.gene027565.t1 AT1G01430 38.760 387 222 8 52 431 69 447 4.97e-92 286
MS.gene027565.t1 AT3G02440 36.524 397 218 7 60 432 83 469 2.75e-91 285
MS.gene027565.t1 AT3G02440 36.524 397 218 7 60 432 91 477 3.92e-91 284
MS.gene027565.t1 AT4G01080 37.940 398 224 8 35 429 51 428 9.61e-91 282
MS.gene027565.t1 AT3G28150 40.227 353 197 8 85 433 70 412 1.19e-89 278
MS.gene027565.t1 AT4G23790 36.385 426 252 11 15 431 12 427 7.08e-89 277
MS.gene027565.t1 AT4G11090 35.880 432 256 12 9 431 6 425 5.66e-87 272
MS.gene027565.t1 AT5G15900 37.467 379 219 10 65 435 45 413 4.94e-83 261
MS.gene027565.t1 AT5G15900 37.467 379 219 10 65 435 64 432 9.50e-83 261
MS.gene027565.t1 AT4G25360 33.249 397 236 11 51 433 145 526 8.44e-70 230
MS.gene027565.t1 AT4G25360 33.249 397 236 11 51 433 145 526 8.44e-70 230
MS.gene027565.t1 AT5G51640 34.409 372 208 12 82 433 135 490 1.22e-66 221
MS.gene027565.t1 AT5G19160 35.171 381 213 12 73 435 84 448 2.17e-58 198
MS.gene027565.t1 AT3G06080 31.956 363 219 10 85 435 103 449 2.55e-52 182
MS.gene027565.t1 AT3G12060 32.075 371 218 15 77 433 188 538 5.23e-50 178
MS.gene027565.t1 AT5G06700 32.493 357 210 14 83 427 253 590 5.33e-47 171
MS.gene027565.t1 AT5G64020 31.351 370 213 12 85 431 57 408 2.05e-46 165
MS.gene027565.t1 AT1G48880 34.043 376 196 17 71 428 95 436 9.15e-46 164
MS.gene027565.t1 AT5G20590 32.055 365 210 14 78 427 134 475 2.57e-44 161
MS.gene027565.t1 AT3G11570 32.249 369 200 15 85 431 79 419 4.09e-44 159
MS.gene027565.t1 AT3G62390 30.226 354 216 12 83 427 136 467 1.15e-42 157
MS.gene027565.t1 AT2G14530 29.286 420 236 19 36 431 24 406 2.44e-42 154
MS.gene027565.t1 AT5G58600 30.108 372 226 11 68 431 55 400 6.32e-42 153
MS.gene027565.t1 AT5G49340 29.859 355 222 11 84 427 99 437 8.70e-42 154
MS.gene027565.t1 AT3G14850 32.869 359 203 11 79 431 28 354 3.23e-41 150
MS.gene027565.t1 AT5G06230 30.295 373 209 14 83 431 19 364 1.81e-40 148
MS.gene027565.t1 AT5G06230 30.481 374 207 15 83 431 60 405 2.83e-40 149
MS.gene027565.t1 AT2G31110 30.748 361 212 15 72 427 31 358 2.22e-39 145
MS.gene027565.t1 AT2G38320 28.729 362 229 12 80 427 54 400 3.58e-39 145
MS.gene027565.t1 AT5G20680 26.952 397 246 12 57 431 83 457 5.49e-39 146
MS.gene027565.t1 AT5G20680 27.204 397 245 11 57 431 159 533 1.77e-38 146
MS.gene027565.t1 AT5G20680 27.204 397 245 11 57 431 177 551 2.12e-38 146
MS.gene027565.t1 AT5G20680 27.204 397 245 11 57 431 177 551 2.12e-38 146
MS.gene027565.t1 AT5G20680 27.204 397 245 11 57 431 177 551 2.12e-38 146
MS.gene027565.t1 AT5G20680 27.204 397 245 11 57 431 177 551 2.12e-38 146
MS.gene027565.t1 AT5G19160 37.931 261 140 10 73 320 84 335 3.57e-38 142
MS.gene027565.t1 AT2G37720 29.076 368 216 11 83 431 141 482 6.59e-38 144
MS.gene027565.t1 AT2G30900 30.312 353 208 12 82 427 40 361 1.02e-37 141
MS.gene027565.t1 AT2G30010 28.415 366 224 10 82 431 53 396 2.43e-37 140
MS.gene027565.t1 AT2G30010 28.610 374 209 10 84 431 98 439 5.24e-37 140
MS.gene027565.t1 AT1G73140 28.809 361 219 14 82 427 64 401 2.22e-36 138
MS.gene027565.t1 AT5G01360 27.424 361 228 12 83 428 34 375 2.91e-36 137
MS.gene027565.t1 AT3G55990 28.691 359 225 12 83 427 138 479 4.60e-36 139
MS.gene027565.t1 AT2G42570 30.726 358 197 16 85 431 47 364 4.62e-36 136
MS.gene027565.t1 AT1G60790 26.975 367 231 13 83 432 188 534 6.15e-36 139
MS.gene027565.t1 AT5G64470 28.889 360 223 14 85 431 55 394 9.28e-36 136
MS.gene027565.t1 AT5G01360 27.424 361 228 12 83 428 90 431 9.79e-36 137
MS.gene027565.t1 AT1G29050 30.199 351 208 13 85 431 61 378 1.94e-35 135
MS.gene027565.t1 AT3G11030 27.778 360 226 12 83 427 104 444 2.71e-35 136
MS.gene027565.t1 AT2G38320 29.141 326 203 11 115 427 7 317 3.18e-35 133
MS.gene027565.t1 AT2G40160 27.945 365 219 14 85 427 77 419 5.19e-35 135
MS.gene027565.t1 AT2G40160 27.945 365 219 14 85 427 77 419 8.09e-35 134
MS.gene027565.t1 AT2G40320 27.835 388 228 14 67 427 56 418 9.36e-35 134
MS.gene027565.t1 AT2G34070 29.745 353 210 15 84 431 64 383 1.30e-34 133
MS.gene027565.t1 AT5G64470 28.611 360 230 14 85 431 55 400 2.59e-34 132
MS.gene027565.t1 AT2G40150 28.682 387 230 16 63 427 54 416 1.58e-33 130
MS.gene027565.t1 AT3G06080 32.389 247 148 6 85 321 103 340 8.66e-33 127
MS.gene027565.t1 AT3G54260 28.453 362 206 15 85 431 51 374 3.41e-32 126
MS.gene027565.t1 AT1G78710 29.917 361 215 15 76 431 29 356 3.24e-31 123
MS.gene027565.t1 AT3G62390 30.081 246 159 6 83 322 136 374 2.47e-29 119
MS.gene027565.t1 AT5G01620 25.729 377 229 14 77 431 110 457 2.67e-28 116
MS.gene027565.t1 AT5G01620 25.729 377 229 14 77 431 102 449 3.00e-28 116
MS.gene027565.t1 AT5G01620 25.729 377 229 14 77 431 102 449 3.00e-28 116
MS.gene027565.t1 AT5G20590 32.411 253 144 10 83 321 139 378 8.04e-28 114
MS.gene027565.t1 AT5G58600 31.621 253 154 8 68 319 55 289 2.71e-26 108
MS.gene027565.t1 AT5G06230 34.975 203 104 8 83 262 60 257 1.14e-24 103
MS.gene027565.t1 AT2G31110 33.152 184 115 4 72 254 31 207 1.42e-24 102
MS.gene027565.t1 AT2G40160 37.405 131 80 2 85 214 77 206 2.98e-24 102
MS.gene027565.t1 AT2G30900 31.925 213 132 5 82 292 40 241 6.58e-23 98.2
MS.gene027565.t1 AT5G64470 28.030 264 168 11 85 335 55 309 2.12e-19 89.0
MS.gene027565.t1 AT5G64470 28.030 264 168 11 85 335 55 309 2.54e-19 89.0
MS.gene027565.t1 AT3G14850 30.496 282 158 11 156 431 2 251 2.67e-19 87.4
MS.gene027565.t1 AT1G78710 32.222 180 113 4 76 254 22 193 6.95e-19 87.0
MS.gene027565.t1 AT2G34070 28.520 277 160 15 156 427 2 245 4.21e-16 78.2
MS.gene027565.t1 AT2G31110 27.468 233 133 13 199 427 10 210 4.14e-11 62.8

Find 96 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTCAATTATACTATTA+AGG 0.109514 5.1:+25920036 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5.1:-25920553 MS.gene027565:CDS
TATGAGAGTGATTTAATTCT+TGG 0.184685 5.1:-25920134 MS.gene027565:CDS
GTCCTCAAAGCCTTTCTTAA+TGG 0.190045 5.1:+25920062 None:intergenic
GTGCCACTTACGAAATTTAT+TGG 0.202356 5.1:+25920352 None:intergenic
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5.1:+25920423 None:intergenic
ACAAATGGAAGACCTGATTT+AGG 0.298411 5.1:-25920566 MS.gene027565:CDS
ATTTATTGGTCTTGCCATCT+TGG 0.331263 5.1:+25920366 None:intergenic
TCTCTCAATTATACTATTAA+GGG 0.345275 5.1:+25920037 None:intergenic
TCAACACCATGCACTAGAAA+TGG 0.356439 5.1:+25920290 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5.1:-25919881 MS.gene027565:CDS
AATCTGAGTATGATTAAAAT+CGG 0.358474 5.1:+25920097 None:intergenic
TTGTAGAGATGATGAAGAAA+TGG 0.363410 5.1:-25919635 MS.gene027565:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5.1:-25920017 MS.gene027565:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5.1:+25921020 None:intergenic
GTGACTATTCAAATGGAGAT+TGG 0.369081 5.1:-25920661 MS.gene027565:CDS
GGTGTTGGATGTAACTAAAT+TGG 0.388499 5.1:-25919793 MS.gene027565:CDS
GACATGATTGTTATATCAAC+CGG 0.390990 5.1:-25920185 MS.gene027565:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5.1:+25920621 None:intergenic
AGAAAAGAGGATAAGATAGT+TGG 0.399914 5.1:-25919906 MS.gene027565:CDS
GTTTGTTCTTAATGAGTTTG+AGG 0.410280 5.1:+25920480 None:intergenic
CAACACCATGCACTAGAAAT+GGG 0.415330 5.1:+25920291 None:intergenic
TACAAAATGATTGTGTTCAT+TGG 0.415725 5.1:-25919695 MS.gene027565:CDS
TGTGTTCATTGGTGCTTGCC+AGG 0.419306 5.1:-25919684 MS.gene027565:CDS
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5.1:+25920380 None:intergenic
GGCAAAGGATATTGATCAAA+TGG 0.424223 5.1:-25920207 MS.gene027565:CDS
TTCCATTAAGAAAGGCTTTG+AGG 0.431515 5.1:-25920064 MS.gene027565:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5.1:+25919723 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5.1:+25920146 None:intergenic
GGATAAGATAGTTGGAGGAA+TGG 0.436305 5.1:-25919898 MS.gene027565:CDS
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5.1:+25919729 None:intergenic
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5.1:+25920928 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5.1:+25920620 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5.1:+25920554 None:intergenic
ACAAAAGCTAATAAATTTGA+AGG 0.447815 5.1:-25919828 MS.gene027565:CDS
AGTGAGAGATGCATCACAAA+TGG 0.449095 5.1:-25920581 MS.gene027565:CDS
TGTAGAGATGATGAAGAAAT+GGG 0.451545 5.1:-25919634 MS.gene027565:CDS
CTTGGTAGATTACACTCACT+TGG 0.451639 5.1:+25920521 None:intergenic
TGACTATTCAAATGGAGATT+GGG 0.453167 5.1:-25920660 MS.gene027565:CDS
ATTTGAAGGTATTAGAATTG+AGG 0.457268 5.1:-25919814 MS.gene027565:CDS
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5.1:-25920547 MS.gene027565:CDS
ATGAGTTTGAGGAAAGTGTT+TGG 0.469541 5.1:+25920491 None:intergenic
AAAGAGGATAAGATAGTTGG+AGG 0.471238 5.1:-25919903 MS.gene027565:CDS
AAATCATGTGACTATTCAAA+TGG 0.471379 5.1:-25920668 MS.gene027565:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5.1:-25920296 MS.gene027565:CDS
TAGTTGGTCCAAAAGGAGTA+AGG 0.483038 5.1:+25920982 None:intergenic
AGAAGCTTAGTTGGTCCAAA+AGG 0.489769 5.1:+25920975 None:intergenic
TAAGGACCAGGATGACCATC+TGG 0.489848 5.1:+25919759 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.491810 5.1:-25919919 MS.gene027565:CDS
GGAGGGAAGATTGTCATTGA+AGG 0.493467 5.1:+25921085 None:intergenic
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5.1:-25920380 MS.gene027565:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5.1:+25919741 None:intergenic
AAGATAGTTGGAGGAATGGA+TGG 0.499482 5.1:-25919894 MS.gene027565:CDS
TGCCAGGACCTATAGATACT+TGG 0.502047 5.1:-25919668 MS.gene027565:CDS
GGATGAAGTATAAACAAAGA+AGG 0.502496 5.1:+25921012 None:intergenic
AAAGTGTTTGGTTCAAACCT+TGG 0.502605 5.1:+25920503 None:intergenic
GAGGGAAGATTGTCATTGAA+GGG 0.504425 5.1:+25921086 None:intergenic
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5.1:+25920932 None:intergenic
TTACCTACAAAAGAAGTTGA+AGG 0.508211 5.1:+25920900 None:intergenic
GTAGAATGAAGAAGCTTAGT+TGG 0.508993 5.1:+25920966 None:intergenic
CAAAAGGAGTAAGGGACAAA+AGG 0.511314 5.1:+25920991 None:intergenic
TTGTTGGAGATTCTTTGGCT+AGG 0.514286 5.1:-25920448 MS.gene027565:CDS
ATCTGAGTATGATTAAAATC+GGG 0.514293 5.1:+25920098 None:intergenic
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5.1:-25920023 MS.gene027565:CDS
CTCTTTGATTGTGCCACATG+TGG 0.519016 5.1:+25920604 None:intergenic
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5.1:-25919954 MS.gene027565:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5.1:-25920275 MS.gene027565:CDS
TTGAGACCAGATGGTCATCC+TGG 0.524393 5.1:-25919765 MS.gene027565:CDS
TGGGGACCAATACAAAGAAA+AGG 0.526383 5.1:+25920310 None:intergenic
GATGAAGTATAAACAAAGAA+GGG 0.527867 5.1:+25921013 None:intergenic
TTGTTATATCAACCGGGCAT+TGG 0.533327 5.1:-25920178 MS.gene027565:CDS
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5.1:+25919666 None:intergenic
AAGGATTGTGATGGAAGAAG+TGG 0.538045 5.1:-25919862 MS.gene027565:CDS
ATCTCATTCCAAGTATCTAT+AGG 0.540214 5.1:+25919660 None:intergenic
AGACCAATAAATTTCGTAAG+TGG 0.541481 5.1:-25920355 MS.gene027565:CDS
GGATCTTGTCAATGAGAGGT+GGG 0.541719 5.1:-25920228 MS.gene027565:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5.1:+25919966 None:intergenic
TGGATCTTGTCAATGAGAGG+TGG 0.557432 5.1:-25920229 MS.gene027565:CDS
AGGTATTAGAATTGAGGTGT+TGG 0.565513 5.1:-25919808 MS.gene027565:CDS
TCTTGCCATCTTGGTAAACA+AGG 0.568916 5.1:+25920375 None:intergenic
GAAAAGGTAGCATTATGAGA+AGG 0.570303 5.1:+25920326 None:intergenic
TGAATAGTGGTAGGTGGCAA+TGG 0.576540 5.1:+25920941 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5.1:-25919774 MS.gene027565:CDS
TGTCAATGAGAGGTGGGCAA+AGG 0.593687 5.1:-25920222 MS.gene027565:CDS
AAAGGATTCATGTAAGGACC+AGG 0.607854 5.1:+25919747 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.614813 5.1:+25920935 None:intergenic
AGTTGGTCCAAAAGGAGTAA+GGG 0.618383 5.1:+25920983 None:intergenic
CCTACAAAAGAAGTTGAAGG+AGG 0.623616 5.1:+25920903 None:intergenic
TGAGATTAGAAGGATTGTGA+TGG 0.626027 5.1:-25919871 MS.gene027565:CDS
ATATACAATGTCACCACATG+TGG 0.627135 5.1:-25920617 MS.gene027565:CDS
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5.1:-25920274 MS.gene027565:CDS
CAAGTGAGTGTAATCTACCA+AGG 0.650297 5.1:-25920520 MS.gene027565:CDS
AACACCATGCACTAGAAATG+GGG 0.662494 5.1:+25920292 None:intergenic
ACATGATTGTTATATCAACC+GGG 0.662677 5.1:-25920184 MS.gene027565:CDS
GATTGGGTTAGTGACATGAG+AGG 0.690038 5.1:-25920644 MS.gene027565:CDS
ATAGTGGTAGGTGGCAATGG+TGG 0.693322 5.1:+25920944 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5.1:25920481-25920500 MS.gene027565:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5.1:25919669-25919688 None:intergenic 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5.1:25920015-25920034 MS.gene027565:CDS 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5.1:25920014-25920033 MS.gene027565:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5.1:25919666-25919685 None:intergenic 20.0%
!! AAAAATTTCTTATTGAAGGA+GGG + chr5.1:25919665-25919684 None:intergenic 20.0%
!! AATCTGAGTATGATTAAAAT+CGG + chr5.1:25920636-25920655 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG - chr5.1:25920902-25920921 MS.gene027565:intron 20.0%
!! TCTCTCAATTATACTATTAA+GGG + chr5.1:25920696-25920715 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTA+AGG + chr5.1:25920697-25920716 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG + chr5.1:25920900-25920919 None:intergenic 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5.1:25921028-25921047 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5.1:25920658-25920677 MS.gene027565:CDS 20.0%
! AAATCATGTGACTATTCAAA+TGG - chr5.1:25920062-25920081 MS.gene027565:CDS 25.0%
! ATCTGAGTATGATTAAAATC+GGG + chr5.1:25920635-25920654 None:intergenic 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5.1:25920179-25920198 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5.1:25919720-25919739 None:intergenic 25.0%
! TACAAAATGATTGTGTTCAT+TGG - chr5.1:25921035-25921054 MS.gene027565:CDS 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5.1:25919713-25919732 None:intergenic 25.0%
! TATGAGAGTGATTTAATTCT+TGG - chr5.1:25920596-25920615 MS.gene027565:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5.1:25920266-25920285 MS.gene027565:CDS 25.0%
!! ATCTTATCCTCTTTTCTATA+AGG + chr5.1:25920821-25920840 None:intergenic 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5.1:25920177-25920196 MS.gene027565:CDS 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG - chr5.1:25920916-25920935 MS.gene027565:CDS 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5.1:25919971-25919990 MS.gene027565:CDS 25.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5.1:25919805-25919824 None:intergenic 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5.1:25920992-25921011 None:intergenic 30.0%
ACATGATTGTTATATCAACC+GGG - chr5.1:25920546-25920565 MS.gene027565:CDS 30.0%
AGAAAAGAGGATAAGATAGT+TGG - chr5.1:25920824-25920843 MS.gene027565:intron 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5.1:25920713-25920732 MS.gene027565:intron 30.0%
AGACCAATAAATTTCGTAAG+TGG - chr5.1:25920375-25920394 MS.gene027565:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5.1:25921073-25921092 None:intergenic 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5.1:25920112-25920131 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5.1:25920707-25920726 MS.gene027565:intron 30.0%
GACATGATTGTTATATCAAC+CGG - chr5.1:25920545-25920564 MS.gene027565:CDS 30.0%
GGATGAAGTATAAACAAAGA+AGG + chr5.1:25919721-25919740 None:intergenic 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5.1:25920456-25920475 MS.gene027565:CDS 30.0%
GTTTGTTCTTAATGAGTTTG+AGG + chr5.1:25920253-25920272 None:intergenic 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5.1:25920183-25920202 MS.gene027565:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5.1:25920587-25920606 None:intergenic 30.0%
TGACTATTCAAATGGAGATT+GGG - chr5.1:25920070-25920089 MS.gene027565:CDS 30.0%
TGTAGAGATGATGAAGAAAT+GGG - chr5.1:25921096-25921115 MS.gene027565:CDS 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5.1:25919980-25919999 MS.gene027565:CDS 30.0%
TTACCTACAAAAGAAGTTGA+AGG + chr5.1:25919833-25919852 None:intergenic 30.0%
TTGTAGAGATGATGAAGAAA+TGG - chr5.1:25921095-25921114 MS.gene027565:CDS 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5.1:25920998-25921017 MS.gene027565:CDS 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG - chr5.1:25920414-25920433 MS.gene027565:CDS 30.0%
!!! ATTTTTTGCAGGGAATAACA+AGG - chr5.1:25920025-25920044 MS.gene027565:CDS 30.0%
AAAGAGGATAAGATAGTTGG+AGG - chr5.1:25920827-25920846 MS.gene027565:intron 35.0%
ACAAATGGAAGACCTGATTT+AGG - chr5.1:25920164-25920183 MS.gene027565:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5.1:25921004-25921023 None:intergenic 35.0%
AGGTATTAGAATTGAGGTGT+TGG - chr5.1:25920922-25920941 MS.gene027565:CDS 35.0%
ATATACAATGTCACCACATG+TGG - chr5.1:25920113-25920132 MS.gene027565:CDS 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5.1:25920113-25920132 None:intergenic 35.0%
GAAAAGGTAGCATTATGAGA+AGG + chr5.1:25920407-25920426 None:intergenic 35.0%
GGCAAAGGATATTGATCAAA+TGG - chr5.1:25920523-25920542 MS.gene027565:CDS 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5.1:25919801-25919820 None:intergenic 35.0%
GTAGAATGAAGAAGCTTAGT+TGG + chr5.1:25919767-25919786 None:intergenic 35.0%
GTGACTATTCAAATGGAGAT+TGG - chr5.1:25920069-25920088 MS.gene027565:CDS 35.0%
TGAGATTAGAAGGATTGTGA+TGG - chr5.1:25920859-25920878 MS.gene027565:intron 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5.1:25920310-25920329 None:intergenic 35.0%
TTCCATTAAGAAAGGCTTTG+AGG - chr5.1:25920666-25920685 MS.gene027565:CDS 35.0%
! AAAGTGTTTGGTTCAAACCT+TGG + chr5.1:25920230-25920249 None:intergenic 35.0%
! AGCTTTTGTTGGAGATTCTT+TGG - chr5.1:25920277-25920296 MS.gene027565:CDS 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5.1:25920811-25920830 MS.gene027565:intron 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5.1:25920455-25920474 MS.gene027565:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG - chr5.1:25920937-25920956 MS.gene027565:CDS 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5.1:25921010-25921029 None:intergenic 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5.1:25920758-25920777 MS.gene027565:intron 35.0%
! TTTTGGATCTTGTCAATGAG+AGG - chr5.1:25920498-25920517 MS.gene027565:CDS 35.0%
!! ATGAGTTTGAGGAAAGTGTT+TGG + chr5.1:25920242-25920261 None:intergenic 35.0%
!! ATTTATTGGTCTTGCCATCT+TGG + chr5.1:25920367-25920386 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5.1:25920381-25920400 None:intergenic 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5.1:25920766-25920785 MS.gene027565:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5.1:25920772-25920791 MS.gene027565:intron 35.0%
AAAGGATTCATGTAAGGACC+AGG + chr5.1:25920986-25921005 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5.1:25920441-25920460 None:intergenic 40.0%
AAGATAGTTGGAGGAATGGA+TGG - chr5.1:25920836-25920855 MS.gene027565:intron 40.0%
AGAAGCTTAGTTGGTCCAAA+AGG + chr5.1:25919758-25919777 None:intergenic 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5.1:25919798-25919817 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5.1:25920149-25920168 MS.gene027565:CDS 40.0%
AGTTGGTCCAAAAGGAGTAA+GGG + chr5.1:25919750-25919769 None:intergenic 40.0%
CAAAAGGAGTAAGGGACAAA+AGG + chr5.1:25919742-25919761 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5.1:25920442-25920461 None:intergenic 40.0%
CAAGTGAGTGTAATCTACCA+AGG - chr5.1:25920210-25920229 MS.gene027565:CDS 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5.1:25920767-25920786 None:intergenic 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5.1:25920353-25920372 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5.1:25919830-25919849 None:intergenic 40.0%
CTTGGTAGATTACACTCACT+TGG + chr5.1:25920212-25920231 None:intergenic 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5.1:25920849-25920868 MS.gene027565:intron 40.0%
GAGGGAAGATTGTCATTGAA+GGG + chr5.1:25919647-25919666 None:intergenic 40.0%
GGATAAGATAGTTGGAGGAA+TGG - chr5.1:25920832-25920851 MS.gene027565:intron 40.0%
GTCCTCAAAGCCTTTCTTAA+TGG + chr5.1:25920671-25920690 None:intergenic 40.0%
TAGTTGGTCCAAAAGGAGTA+AGG + chr5.1:25919751-25919770 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5.1:25920443-25920462 None:intergenic 40.0%
TCTTGCCATCTTGGTAAACA+AGG + chr5.1:25920358-25920377 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG + chr5.1:25920423-25920442 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5.1:25921067-25921086 None:intergenic 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5.1:25920956-25920975 MS.gene027565:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5.1:25920552-25920571 MS.gene027565:CDS 40.0%
TTGTTGGAGATTCTTTGGCT+AGG - chr5.1:25920282-25920301 MS.gene027565:CDS 40.0%
! AAGGATTGTGATGGAAGAAG+TGG - chr5.1:25920868-25920887 MS.gene027565:intron 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5.1:25920350-25920369 MS.gene027565:CDS 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5.1:25919827-25919846 MS.gene027565:CDS 40.0%
! CTTTTGTCCCTTACTCCTTT+TGG - chr5.1:25919740-25919759 MS.gene027565:CDS 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5.1:25920765-25920784 MS.gene027565:intron 40.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5.1:25920567-25920586 None:intergenic 45.0%
CTCTTTGATTGTGCCACATG+TGG + chr5.1:25920129-25920148 None:intergenic 45.0%
GGAGGGAAGATTGTCATTGA+AGG + chr5.1:25919648-25919667 None:intergenic 45.0%
GGATCTTGTCAATGAGAGGT+GGG - chr5.1:25920502-25920521 MS.gene027565:CDS 45.0%
TGAATAGTGGTAGGTGGCAA+TGG + chr5.1:25919792-25919811 None:intergenic 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5.1:25921062-25921081 MS.gene027565:CDS 45.0%
TGGATCTTGTCAATGAGAGG+TGG - chr5.1:25920501-25920520 MS.gene027565:CDS 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5.1:25920086-25920105 MS.gene027565:CDS 45.0%
ATAGTGGTAGGTGGCAATGG+TGG + chr5.1:25919789-25919808 None:intergenic 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5.1:25920776-25920795 MS.gene027565:intron 50.0%
TAAGGACCAGGATGACCATC+TGG + chr5.1:25920974-25920993 None:intergenic 50.0%
TGTCAATGAGAGGTGGGCAA+AGG - chr5.1:25920508-25920527 MS.gene027565:CDS 50.0%
TTGAGACCAGATGGTCATCC+TGG - chr5.1:25920965-25920984 MS.gene027565:CDS 50.0%
! TGTGTTCATTGGTGCTTGCC+AGG - chr5.1:25921046-25921065 MS.gene027565:CDS 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5.1:25920434-25920453 MS.gene027565:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 25919629 25921123 25919629 ID=MS.gene027565
chr5.1 mRNA 25919629 25921123 25919629 ID=MS.gene027565.t1;Parent=MS.gene027565
chr5.1 exon 25920904 25921123 25920904 ID=MS.gene027565.t1.exon1;Parent=MS.gene027565.t1
chr5.1 CDS 25920904 25921123 25920904 ID=cds.MS.gene027565.t1;Parent=MS.gene027565.t1
chr5.1 exon 25919629 25920716 25919629 ID=MS.gene027565.t1.exon2;Parent=MS.gene027565.t1
chr5.1 CDS 25919629 25920716 25919629 ID=cds.MS.gene027565.t1;Parent=MS.gene027565.t1
Gene Sequence

>MS.gene027565

ATGGGAAGCACAAATCCCTTCAATGACAATCTTCCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCCTTTTGTCCCTTACTCCTTTTGGACCAACTAAGCTTCTTCATTCTACCACCATTGCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGTAAATATGCATGCTGCATAATTAACTTCTAGTTGAGATTCACATGAACTCTAACTTCTATTCATCTACTTTTTTTTTTTAAAAAAAAAAATTATGCTAGTTGAGATTCACATGAACTCTAACTAGCAATTTGTTACTATTAAGGACTTCTTGGTTTACTAATTTTTATGTAATATATTTTTTGCAGGGAATAACAAGGTTTATGATTATGAGAAATCATGTGACTATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAGAGTGAGAGATGCATCACAAATGGAAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAATCTACCAAGGTTTGAACCAAACACTTTCCTCAAACTCATTAAGAACAAACATATAGCTTTTGTTGGAGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTAGCAGCTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGACCAATAAATTTCGTAAGTGGCACTTTCCTTCTCATAATGCTACCTTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAGGTGGGCAAAGGATATTGATCAAATGGACATGATTGTTATATCAACCGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAATTCTTGGTAGCTTGAATTGTCCCGATTTTAATCATACTCAGATTGATTTTTATGTTCCATTAAGAAAGGCTTTGAGGACTACCCTTAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGATAAGATAGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGTGATGGAAGAAGTGGAAAGTGCCAAGACAAAAGCTAATAAATTTGAAGGTATTAGAATTGAGGTGTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCTGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAATGATTGTGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTCTTGTAGAGATGATGAAGAAATGGGAGTAA
Protein sequence

>MS.gene027565.t1

MGSTNPFNDNLPSFNKKFFHLTLYSLLPISLLCLYFILLSLTPFGPTKLLHSTTIATYHYSSTYSSPPSTSFVGNNKVYDYEKSCDYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECNLPRFEPNTFLKLIKNKHIAFVGDSLARNQLESLLCMLAATSTPNLVYQDGKTNKFRKWHFPSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNERWAKDIDQMDMIVISTGHWFLLPSIYYESDLILGSLNCPDFNHTQIDFYVPLRKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEDKIVGGMDGEIRRIVMEEVESAKTKANKFEGIRIEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEILVEMMKKWE