AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027568

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027568.t1 MTR_5g035150 96.322 435 16 0 1 435 1 435 0.0 882
MS.gene027568.t1 MTR_5g035070 65.287 435 149 2 1 435 5 437 0.0 568
MS.gene027568.t1 MTR_5g035090 62.989 435 155 3 1 435 3 431 0.0 554
MS.gene027568.t1 MTR_5g035080 66.914 405 114 6 34 435 37 424 0.0 545
MS.gene027568.t1 MTR_3g109840 62.615 436 136 9 1 433 6 417 0.0 535
MS.gene027568.t1 MTR_8g104970 57.359 462 147 9 16 432 10 466 0.0 520
MS.gene027568.t1 MTR_7g033230 62.535 355 126 4 82 433 261 611 3.40e-163 473
MS.gene027568.t1 MTR_7g033230 62.535 355 126 4 82 433 331 681 2.59e-162 473
MS.gene027568.t1 MTR_5g035140 66.038 265 83 4 172 433 7 267 2.28e-128 372
MS.gene027568.t1 MTR_8g099300 42.330 352 192 7 85 431 74 419 2.38e-97 298
MS.gene027568.t1 MTR_8g027900 39.362 376 213 6 60 431 191 555 1.38e-96 300
MS.gene027568.t1 MTR_6g016880 37.831 415 240 10 25 432 17 420 5.14e-95 293
MS.gene027568.t1 MTR_7g019120 35.835 413 243 10 29 432 22 421 2.45e-89 278
MS.gene027568.t1 MTR_6g016875 38.814 371 213 7 68 432 47 409 9.75e-85 266
MS.gene027568.t1 MTR_6g007777 38.172 372 212 8 66 433 102 459 3.33e-84 266
MS.gene027568.t1 MTR_2g016060 37.640 356 197 12 90 433 60 402 1.21e-76 244
MS.gene027568.t1 MTR_1g076690 33.609 363 214 11 85 433 223 572 2.07e-65 220
MS.gene027568.t1 MTR_6g061710 31.989 372 212 11 83 433 165 516 1.11e-63 214
MS.gene027568.t1 MTR_7g094750 33.333 369 203 15 84 433 90 434 9.80e-53 183
MS.gene027568.t1 MTR_2g022560 33.423 371 203 14 84 435 96 441 3.10e-49 174
MS.gene027568.t1 MTR_5g035120 50.000 172 75 3 4 175 5 165 4.45e-48 162
MS.gene027568.t1 MTR_0136s0010 33.060 366 205 14 85 433 170 512 7.82e-48 171
MS.gene027568.t1 MTR_7g093450 31.343 402 239 14 43 427 202 583 2.07e-46 169
MS.gene027568.t1 MTR_4g126950 32.044 362 211 13 83 431 107 446 4.94e-46 165
MS.gene027568.t1 MTR_5g059400 30.579 363 208 14 84 427 174 511 3.21e-43 159
MS.gene027568.t1 MTR_1g116130 30.579 363 208 14 84 427 174 511 3.21e-43 159
MS.gene027568.t1 MTR_8g069790 29.600 375 227 13 73 432 166 518 5.21e-42 155
MS.gene027568.t1 MTR_5g071350 30.110 362 226 10 77 427 50 395 8.39e-42 152
MS.gene027568.t1 MTR_4g125060 29.700 367 220 12 76 427 124 467 8.68e-42 154
MS.gene027568.t1 MTR_4g014440 31.111 360 213 16 85 427 62 403 9.05e-42 153
MS.gene027568.t1 MTR_8g093530 29.609 358 214 10 83 431 88 416 1.06e-41 153
MS.gene027568.t1 MTR_1g058380 28.726 369 216 14 83 431 53 394 1.28e-41 152
MS.gene027568.t1 MTR_4g121560 27.830 424 253 17 27 431 64 453 1.41e-41 153
MS.gene027568.t1 MTR_4g125060 29.700 367 220 12 76 427 163 506 2.30e-41 154
MS.gene027568.t1 MTR_4g125060 29.700 367 220 12 76 427 192 535 2.39e-41 154
MS.gene027568.t1 MTR_7g109550 31.285 358 200 14 85 427 137 463 3.85e-41 152
MS.gene027568.t1 MTR_4g016940 29.255 376 216 16 79 431 79 427 6.63e-41 150
MS.gene027568.t1 MTR_7g068920 31.088 386 214 15 69 431 65 421 3.23e-40 149
MS.gene027568.t1 MTR_7g070060 30.194 361 215 15 85 427 64 405 7.29e-40 147
MS.gene027568.t1 MTR_8g463790 26.835 436 258 16 23 433 12 411 1.89e-39 147
MS.gene027568.t1 MTR_5g033800 27.446 368 229 12 81 433 141 485 5.20e-39 147
MS.gene027568.t1 MTR_2g073510 31.268 355 208 13 82 431 30 353 1.94e-38 142
MS.gene027568.t1 MTR_3g035830 29.248 359 197 11 83 427 29 344 1.11e-37 140
MS.gene027568.t1 MTR_3g464570 30.086 349 225 11 82 427 40 372 2.32e-37 140
MS.gene027568.t1 MTR_3g070900 27.778 360 229 12 82 427 103 445 7.16e-37 140
MS.gene027568.t1 MTR_3g090120 28.831 385 214 13 69 431 113 459 1.48e-36 140
MS.gene027568.t1 MTR_3g021090 29.834 362 191 14 83 427 29 344 4.21e-36 137
MS.gene027568.t1 MTR_3g049330 30.641 359 209 13 76 427 21 346 6.62e-36 135
MS.gene027568.t1 MTR_3g021060 30.460 348 207 12 83 427 44 359 7.89e-36 135
MS.gene027568.t1 MTR_6g016240 28.027 446 266 16 8 431 4 416 1.49e-35 136
MS.gene027568.t1 MTR_2g073500 30.168 358 204 14 82 431 26 345 3.38e-35 134
MS.gene027568.t1 MTR_8g093870 26.722 363 231 9 83 431 131 472 3.92e-35 136
MS.gene027568.t1 MTR_7g094750 33.597 253 139 9 84 321 90 328 4.35e-35 133
MS.gene027568.t1 MTR_4g092830 28.780 410 226 16 54 431 17 392 8.16e-35 134
MS.gene027568.t1 MTR_2g091175 31.988 347 198 13 85 427 50 362 2.21e-34 132
MS.gene027568.t1 MTR_4g088030 30.058 346 209 10 84 427 32 346 3.35e-34 131
MS.gene027568.t1 MTR_4g087730 30.347 346 208 10 84 427 44 358 8.82e-34 130
MS.gene027568.t1 MTR_5g083260 29.111 371 208 13 82 427 80 420 9.59e-34 131
MS.gene027568.t1 MTR_1g050448 28.533 368 231 12 67 431 37 375 2.89e-33 129
MS.gene027568.t1 MTR_3g071650 29.167 360 216 12 85 427 89 426 2.96e-33 130
MS.gene027568.t1 MTR_1g112210 26.776 366 209 13 80 427 54 378 4.02e-32 126
MS.gene027568.t1 MTR_5g082880 28.767 365 220 15 83 427 163 507 4.44e-32 128
MS.gene027568.t1 MTR_4g079700 28.871 381 235 13 55 431 15 363 8.96e-32 124
MS.gene027568.t1 MTR_2g015720 29.945 364 219 14 70 431 29 358 1.76e-31 124
MS.gene027568.t1 MTR_5g034810 28.857 350 210 13 83 427 33 348 2.82e-31 123
MS.gene027568.t1 MTR_1g112210 25.627 359 209 11 80 427 54 365 2.96e-30 120
MS.gene027568.t1 MTR_4g014430 27.568 370 218 16 83 431 106 446 3.36e-29 119
MS.gene027568.t1 MTR_7g019140 37.086 151 86 3 29 174 22 168 1.03e-28 111
MS.gene027568.t1 MTR_1g111010 28.219 365 213 15 73 431 20 341 8.07e-28 113
MS.gene027568.t1 MTR_5g033800 31.667 180 116 3 81 254 141 319 1.08e-26 110
MS.gene027568.t1 MTR_7g070060 28.617 311 180 15 137 427 15 303 3.20e-24 102
MS.gene027568.t1 MTR_7g070060 30.488 246 146 9 85 319 64 295 1.34e-23 100
MS.gene027568.t1 MTR_4g102570 38.583 127 66 3 201 321 2 122 3.86e-22 92.8
MS.gene027568.t1 MTR_3g464570 28.369 282 183 11 149 427 3 268 7.11e-21 92.4
MS.gene027568.t1 MTR_7g117160 33.163 196 78 8 235 430 47 189 1.03e-19 87.0
MS.gene027568.t1 MTR_7g019130 31.200 125 79 3 206 326 4 125 5.58e-13 66.6
MS.gene027568.t1 MTR_0929s0010 31.818 110 66 3 29 133 22 127 1.45e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027568.t1 AT1G70230 49.767 430 190 10 10 435 8 415 1.18e-150 434
MS.gene027568.t1 AT5G15890 40.052 387 213 8 50 432 152 523 9.84e-100 308
MS.gene027568.t1 AT1G01430 39.276 387 220 8 52 431 69 447 2.39e-93 289
MS.gene027568.t1 AT4G01080 38.191 398 223 8 35 429 51 428 4.38e-92 285
MS.gene027568.t1 AT3G02440 39.326 356 195 7 85 432 127 469 6.39e-92 286
MS.gene027568.t1 AT3G02440 39.326 356 195 7 85 432 135 477 8.63e-92 286
MS.gene027568.t1 AT4G23790 36.879 423 254 9 15 431 12 427 9.64e-92 284
MS.gene027568.t1 AT3G28150 37.284 405 236 10 36 433 19 412 7.39e-91 281
MS.gene027568.t1 AT4G11090 36.574 432 253 12 9 431 6 425 3.65e-89 278
MS.gene027568.t1 AT5G15900 37.467 379 219 10 65 435 45 413 2.60e-83 262
MS.gene027568.t1 AT5G15900 37.467 379 219 10 65 435 64 432 4.84e-83 262
MS.gene027568.t1 AT4G25360 33.501 397 235 11 51 433 145 526 7.35e-71 233
MS.gene027568.t1 AT4G25360 33.501 397 235 11 51 433 145 526 7.35e-71 233
MS.gene027568.t1 AT5G51640 34.615 390 222 14 63 433 115 490 3.09e-66 220
MS.gene027568.t1 AT5G19160 34.699 366 208 11 85 435 99 448 3.43e-56 192
MS.gene027568.t1 AT3G06080 31.956 363 219 11 85 435 103 449 8.35e-51 178
MS.gene027568.t1 AT3G12060 32.345 371 217 15 77 433 188 538 5.39e-50 178
MS.gene027568.t1 AT1G48880 34.483 377 193 17 71 428 95 436 2.12e-46 166
MS.gene027568.t1 AT5G06700 31.933 357 212 13 83 427 253 590 3.36e-46 169
MS.gene027568.t1 AT5G64020 31.081 370 214 14 85 431 57 408 1.24e-45 163
MS.gene027568.t1 AT3G11570 32.249 369 200 15 85 431 79 419 2.30e-44 160
MS.gene027568.t1 AT5G20590 31.522 368 214 13 75 427 131 475 1.71e-43 159
MS.gene027568.t1 AT2G14530 29.524 420 235 19 36 431 24 406 5.60e-43 156
MS.gene027568.t1 AT3G14850 33.148 359 202 11 79 431 28 354 3.23e-41 150
MS.gene027568.t1 AT5G49340 29.859 355 222 11 84 427 99 437 3.34e-41 152
MS.gene027568.t1 AT3G62390 29.834 362 207 13 83 427 136 467 3.35e-41 152
MS.gene027568.t1 AT5G58600 29.839 372 227 11 68 431 55 400 2.07e-40 149
MS.gene027568.t1 AT5G06230 30.319 376 205 16 83 431 19 364 2.85e-39 145
MS.gene027568.t1 AT5G06230 30.504 377 203 17 83 431 60 405 4.19e-39 145
MS.gene027568.t1 AT2G31110 30.194 361 214 15 72 427 31 358 5.78e-39 144
MS.gene027568.t1 AT2G38320 29.006 362 228 13 80 427 54 400 8.78e-39 145
MS.gene027568.t1 AT5G20680 27.204 397 245 11 57 431 83 457 5.11e-38 144
MS.gene027568.t1 AT5G20680 27.204 397 245 11 57 431 159 533 1.36e-37 144
MS.gene027568.t1 AT5G20680 27.204 397 245 11 57 431 177 551 1.54e-37 144
MS.gene027568.t1 AT5G20680 27.204 397 245 11 57 431 177 551 1.54e-37 144
MS.gene027568.t1 AT5G20680 27.204 397 245 11 57 431 177 551 1.54e-37 144
MS.gene027568.t1 AT5G20680 27.204 397 245 11 57 431 177 551 1.54e-37 144
MS.gene027568.t1 AT2G37720 28.804 368 217 11 83 431 141 482 6.08e-37 141
MS.gene027568.t1 AT5G19160 37.398 246 135 9 85 320 99 335 6.35e-37 138
MS.gene027568.t1 AT1G60790 27.248 367 230 13 83 432 188 534 9.18e-37 141
MS.gene027568.t1 AT2G30010 27.926 376 213 10 82 431 53 396 1.10e-36 139
MS.gene027568.t1 AT2G30010 28.075 374 211 10 84 431 98 439 1.22e-36 139
MS.gene027568.t1 AT5G64470 28.611 360 224 13 85 431 55 394 1.89e-36 138
MS.gene027568.t1 AT2G30900 29.745 353 210 12 82 427 40 361 2.69e-36 137
MS.gene027568.t1 AT3G55990 29.363 361 220 14 83 427 138 479 4.88e-36 139
MS.gene027568.t1 AT5G01360 27.978 361 226 13 83 428 34 375 6.18e-36 136
MS.gene027568.t1 AT2G42570 30.618 356 200 16 85 431 47 364 9.60e-36 135
MS.gene027568.t1 AT5G01360 27.978 361 226 13 83 428 90 431 1.92e-35 136
MS.gene027568.t1 AT1G29050 29.167 360 220 12 75 431 51 378 5.79e-35 134
MS.gene027568.t1 AT1G73140 28.841 371 224 15 72 427 56 401 7.20e-35 134
MS.gene027568.t1 AT2G40160 26.995 426 259 16 26 427 22 419 8.51e-35 134
MS.gene027568.t1 AT2G40160 27.765 425 257 17 26 427 22 419 8.57e-35 134
MS.gene027568.t1 AT2G38320 29.448 326 202 12 115 427 7 317 1.20e-34 131
MS.gene027568.t1 AT2G34070 29.661 354 209 14 84 431 64 383 1.47e-34 132
MS.gene027568.t1 AT5G64470 28.333 360 231 13 85 431 55 400 1.68e-34 133
MS.gene027568.t1 AT3G11030 27.654 358 229 11 83 427 104 444 2.32e-34 133
MS.gene027568.t1 AT2G40150 28.831 385 232 16 63 427 54 416 6.90e-34 132
MS.gene027568.t1 AT2G40320 28.142 366 218 13 82 427 78 418 3.26e-33 130
MS.gene027568.t1 AT3G54260 28.453 362 206 15 85 431 51 374 5.25e-32 125
MS.gene027568.t1 AT3G06080 31.579 247 150 6 85 321 103 340 1.04e-31 124
MS.gene027568.t1 AT1G78710 29.917 361 215 15 76 431 29 356 3.64e-30 120
MS.gene027568.t1 AT3G62390 29.675 246 160 6 83 322 136 374 1.22e-28 117
MS.gene027568.t1 AT5G20590 31.801 261 151 10 75 321 131 378 1.11e-27 113
MS.gene027568.t1 AT5G01620 25.067 375 234 13 77 431 110 457 1.68e-26 111
MS.gene027568.t1 AT5G01620 25.067 375 234 13 77 431 102 449 1.85e-26 111
MS.gene027568.t1 AT5G01620 25.067 375 234 13 77 431 102 449 1.85e-26 111
MS.gene027568.t1 AT5G58600 30.830 253 156 8 68 319 55 289 6.87e-25 103
MS.gene027568.t1 AT2G31110 32.609 184 116 4 72 254 31 207 1.41e-24 102
MS.gene027568.t1 AT2G40160 32.292 192 120 4 26 214 22 206 2.41e-24 103
MS.gene027568.t1 AT5G06230 35.294 204 102 9 83 262 60 257 3.10e-24 102
MS.gene027568.t1 AT2G30900 31.455 213 133 5 82 292 40 241 2.33e-22 96.7
MS.gene027568.t1 AT5G64470 27.037 270 175 10 85 341 55 315 8.59e-20 90.1
MS.gene027568.t1 AT5G64470 27.138 269 174 10 85 340 55 314 8.88e-20 90.1
MS.gene027568.t1 AT3G14850 30.496 282 158 11 156 431 2 251 1.94e-19 87.8
MS.gene027568.t1 AT1G78710 31.667 180 114 4 76 254 22 193 2.88e-18 85.5
MS.gene027568.t1 AT2G34070 28.417 278 159 14 156 427 2 245 5.25e-16 77.8

Find 96 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTCAATTATACTATTA+AGG 0.109514 5.1:+25875276 None:intergenic
GTGCCACTTACGAAATTTAT+TGG 0.120096 5.1:+25875592 None:intergenic
CTGATTTAGGTTATCTTTAT+TGG 0.135262 5.1:-25875793 MS.gene027568:CDS
TATGAGAGTGATTTAATTCT+TGG 0.184685 5.1:-25875374 MS.gene027568:CDS
GTCCTCAAAGCCTTTCTTAA+TGG 0.190045 5.1:+25875302 None:intergenic
TGCAAAGAAGTGACTCTAAT+TGG 0.285207 5.1:+25875663 None:intergenic
ACAAATGGTAGACCTGATTT+AGG 0.287971 5.1:-25875806 MS.gene027568:CDS
TCTCTCAATTATACTATTAA+GGG 0.345275 5.1:+25875277 None:intergenic
TCAACACCATGCACTAGAAA+TGG 0.356439 5.1:+25875530 None:intergenic
GAATGGATGGTGAGATTAGA+AGG 0.357080 5.1:-25875121 MS.gene027568:CDS
AATCTGAGTATGATTAAAAT+CGG 0.358474 5.1:+25875337 None:intergenic
TTGTAGAGATGATGAAGAAA+TGG 0.363410 5.1:-25874875 MS.gene027568:CDS
AGAAAAGTTGCTAAAGGAAA+TGG 0.366030 5.1:-25875257 MS.gene027568:CDS
TATAAACAAAGAAGGGATAT+AGG 0.366305 5.1:+25876253 None:intergenic
GTGACTATTCAAATGGAGAT+TGG 0.369081 5.1:-25875901 MS.gene027568:CDS
GGTGTTGGATGTAACTAAAT+TGG 0.388499 5.1:-25875033 MS.gene027568:CDS
GACATGATTGTTATATCAAC+CGG 0.390990 5.1:-25875425 MS.gene027568:CDS
TACAAAATGATTGCGTTCAT+TGG 0.394768 5.1:-25874935 MS.gene027568:CDS
ATGTGGTGACATTGTATATA+GGG 0.397785 5.1:+25875861 None:intergenic
ATTTATTGGACTTGCCATCT+TGG 0.403569 5.1:+25875606 None:intergenic
GTTTGTTCTTAATGAGTTTG+AGG 0.410280 5.1:+25875720 None:intergenic
CAACACCATGCACTAGAAAT+GGG 0.415330 5.1:+25875531 None:intergenic
CCATCTTGGTAAACAAGGTT+AGG 0.420926 5.1:+25875620 None:intergenic
GGCAAAGGATATTGATCAAA+TGG 0.424223 5.1:-25875447 MS.gene027568:CDS
TGCGTTCATTGGTGCTTGCC+AGG 0.424514 5.1:-25874924 MS.gene027568:CDS
GGAGAAGATCGTTGGAGGAA+TGG 0.425369 5.1:-25875138 MS.gene027568:CDS
TTCCATTAAGAAAGGCTTTG+AGG 0.431515 5.1:-25875304 MS.gene027568:CDS
TTTGGAACTCCATTTGCAAA+TGG 0.431908 5.1:+25874963 None:intergenic
TCACTCTCATAGTAAATTGA+AGG 0.436067 5.1:+25875386 None:intergenic
ACTCCATTTGCAAATGGAAA+AGG 0.436668 5.1:+25874969 None:intergenic
AAGAGTAAGTAGATGAATAG+TGG 0.440562 5.1:+25876161 None:intergenic
CATGTGGTGACATTGTATAT+AGG 0.444034 5.1:+25875860 None:intergenic
CAATAAAGATAACCTAAATC+AGG 0.445212 5.1:+25875794 None:intergenic
ACAAAAGCTAATAAATTTGA+AGG 0.447815 5.1:-25875068 MS.gene027568:CDS
TGTAGAGATGATGAAGAAAT+GGG 0.451545 5.1:-25874874 MS.gene027568:CDS
TGACTATTCAAATGGAGATT+GGG 0.453167 5.1:-25875900 MS.gene027568:CDS
AGAAAAGAGGAGAAGATCGT+TGG 0.456788 5.1:-25875146 MS.gene027568:CDS
ATTTGAAGGTATTAGAATTG+AGG 0.457268 5.1:-25875054 MS.gene027568:CDS
TAGGTTATCTTTATTGGAGA+TGG 0.460710 5.1:-25875787 MS.gene027568:CDS
TAAGGACCTGGATGACCATC+TGG 0.461971 5.1:+25874999 None:intergenic
ATGAGTTTGAGGAAAGTGTT+TGG 0.469541 5.1:+25875731 None:intergenic
AAATCATGTGACTATTCAAA+TGG 0.471379 5.1:-25875908 MS.gene027568:CDS
TTGAGACCAGATGGTCATCC+AGG 0.473377 5.1:-25875005 MS.gene027568:CDS
AAGGATTGAGATAGAAGAAG+TGG 0.473823 5.1:-25875102 MS.gene027568:CDS
TGGTCCCCATTTCTAGTGCA+TGG 0.482297 5.1:-25875536 MS.gene027568:CDS
TAGTTGGTCCAAAAGGAGTA+AGG 0.483038 5.1:+25876215 None:intergenic
GACTAAGCCTTATAGAAAAG+AGG 0.487707 5.1:-25875159 MS.gene027568:CDS
AAAGAGGAGAAGATCGTTGG+AGG 0.490579 5.1:-25875143 MS.gene027568:CDS
GGAGGGAAGATTGTCATTGA+AGG 0.493467 5.1:+25876318 None:intergenic
CCTAACCTTGTTTACCAAGA+TGG 0.495701 5.1:-25875620 MS.gene027568:CDS
AATGGAAAAGGATTCATGTA+AGG 0.496598 5.1:+25874981 None:intergenic
AGTGAGAGATGCATCACAAA+TGG 0.499969 5.1:-25875821 MS.gene027568:CDS
TGCCAGGACCTATAGATACT+TGG 0.502047 5.1:-25874908 MS.gene027568:CDS
GGATGAAGTATAAACAAAGA+AGG 0.502496 5.1:+25876245 None:intergenic
AAAGTGTTTGGTTCAAACCT+TGG 0.502605 5.1:+25875743 None:intergenic
GAGGGAAGATTGTCATTGAA+GGG 0.504425 5.1:+25876319 None:intergenic
GTAAGTAGATGAATAGTGGT+AGG 0.506588 5.1:+25876165 None:intergenic
AGGAGCTTAGTTGGTCCAAA+AGG 0.507239 5.1:+25876208 None:intergenic
TTACCTACAAAAGAAGTTGA+AGG 0.508211 5.1:+25876133 None:intergenic
CAAAAGGAGTAAGGGACAAA+AGG 0.511314 5.1:+25876224 None:intergenic
GTAGAATGAAGGAGCTTAGT+TGG 0.511810 5.1:+25876199 None:intergenic
TTGTTGGAGATTCTTTGGCT+AGG 0.514286 5.1:-25875688 MS.gene027568:CDS
ATCTGAGTATGATTAAAATC+GGG 0.514293 5.1:+25875338 None:intergenic
ATTGAGAGAAAAGTTGCTAA+AGG 0.514738 5.1:-25875263 MS.gene027568:CDS
CTCTTTGATTGTGCCACATG+TGG 0.519016 5.1:+25875844 None:intergenic
GAAGGTGATTGGGATAAGGC+TGG 0.521359 5.1:-25875194 MS.gene027568:CDS
GGTGTTGAAAGATCAAATGA+AGG 0.522081 5.1:-25875515 MS.gene027568:CDS
CTTGGTAGACTACACTCACT+TGG 0.526149 5.1:+25875761 None:intergenic
TGGGGACCAATACAAAGAAA+AGG 0.526383 5.1:+25875550 None:intergenic
GATGAAGTATAAACAAAGAA+GGG 0.527867 5.1:+25876246 None:intergenic
AGTCCAATAAATTTCGTAAG+TGG 0.528019 5.1:-25875595 MS.gene027568:CDS
AAGATCGTTGGAGGAATGGA+TGG 0.529499 5.1:-25875134 MS.gene027568:CDS
TTGTTATATCAACCGGGCAT+TGG 0.533327 5.1:-25875418 MS.gene027568:CDS
TTCCAAGTATCTATAGGTCC+TGG 0.536833 5.1:+25874906 None:intergenic
ATCTCATTCCAAGTATCTAT+AGG 0.540214 5.1:+25874900 None:intergenic
GGATCTTGTCAATGAGAGGT+GGG 0.541719 5.1:-25875468 MS.gene027568:CDS
CAATCACCTTCAAAATGAGC+AGG 0.550833 5.1:+25875206 None:intergenic
TGGATCTTGTCAATGAGAGG+TGG 0.557432 5.1:-25875469 MS.gene027568:CDS
AGGTATTAGAATTGAGGTGT+TGG 0.565513 5.1:-25875048 MS.gene027568:CDS
GAAAAGGTAGCATTATGAGA+AGG 0.570303 5.1:+25875566 None:intergenic
TGGCAATGGTGGTAGAATGA+AGG 0.572970 5.1:+25876188 None:intergenic
TGAATAGTGGTAGGTGGCAA+TGG 0.576540 5.1:+25876174 None:intergenic
ACTTGCCATCTTGGTAAACA+AGG 0.587524 5.1:+25875615 None:intergenic
TTGGCATTATTGAGACCAGA+TGG 0.590154 5.1:-25875014 MS.gene027568:CDS
TGTCAATGAGAGGTGGGCAA+AGG 0.593687 5.1:-25875462 MS.gene027568:CDS
AAAGGATTCATGTAAGGACC+TGG 0.594011 5.1:+25874987 None:intergenic
AGTAGATGAATAGTGGTAGG+TGG 0.614813 5.1:+25876168 None:intergenic
AGTTGGTCCAAAAGGAGTAA+GGG 0.618383 5.1:+25876216 None:intergenic
CCTACAAAAGAAGTTGAAGG+AGG 0.623616 5.1:+25876136 None:intergenic
ATATACAATGTCACCACATG+TGG 0.627135 5.1:-25875857 MS.gene027568:CDS
GTGTTGAAAGATCAAATGAA+GGG 0.634222 5.1:-25875514 MS.gene027568:CDS
AACACCATGCACTAGAAATG+GGG 0.662494 5.1:+25875532 None:intergenic
ACATGATTGTTATATCAACC+GGG 0.662677 5.1:-25875424 MS.gene027568:CDS
CAAGTGAGTGTAGTCTACCA+AGG 0.667691 5.1:-25875760 MS.gene027568:CDS
GATTGGGTTAGTGACATGAG+AGG 0.690038 5.1:-25875884 MS.gene027568:CDS
ATAGTGGTAGGTGGCAATGG+TGG 0.693322 5.1:+25876177 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATAATACAATGTTTT+TGG - chr5.1:25875714-25875733 MS.gene027568:CDS 10.0%
!! ATGAAAAAATTTCTTATTGA+AGG + chr5.1:25874909-25874928 None:intergenic 15.0%
!!! TATGTAATATATTTTTTGCA+GGG - chr5.1:25875248-25875267 MS.gene027568:CDS 15.0%
!!! TTATGTAATATATTTTTTGC+AGG - chr5.1:25875247-25875266 MS.gene027568:CDS 15.0%
!! AAAAAATTTCTTATTGAAGG+AGG + chr5.1:25874906-25874925 None:intergenic 20.0%
!! AAAAATTTCTTATTGAAGGA+GGG + chr5.1:25874905-25874924 None:intergenic 20.0%
!! AATCTGAGTATGATTAAAAT+CGG + chr5.1:25875869-25875888 None:intergenic 20.0%
!! ACAAAAGCTAATAAATTTGA+AGG - chr5.1:25876135-25876154 MS.gene027568:intron 20.0%
!! TCTCTCAATTATACTATTAA+GGG + chr5.1:25875929-25875948 None:intergenic 20.0%
!! TTCTCTCAATTATACTATTA+AGG + chr5.1:25875930-25875949 None:intergenic 20.0%
!!! AAATTTATTAGCTTTTGTCT+TGG + chr5.1:25876133-25876152 None:intergenic 20.0%
!!! CAATCATTTTGTACATATTT+TGG + chr5.1:25876261-25876280 None:intergenic 20.0%
!!! TTTTTATGTTCCATTAAGAA+AGG - chr5.1:25875891-25875910 MS.gene027568:CDS 20.0%
! AAATCATGTGACTATTCAAA+TGG - chr5.1:25875295-25875314 MS.gene027568:CDS 25.0%
! ATCTGAGTATGATTAAAATC+GGG + chr5.1:25875868-25875887 None:intergenic 25.0%
! CAATAAAGATAACCTAAATC+AGG + chr5.1:25875412-25875431 None:intergenic 25.0%
! GATGAAGTATAAACAAAGAA+GGG + chr5.1:25874960-25874979 None:intergenic 25.0%
! TATAAACAAAGAAGGGATAT+AGG + chr5.1:25874953-25874972 None:intergenic 25.0%
! TATGAGAGTGATTTAATTCT+TGG - chr5.1:25875829-25875848 MS.gene027568:CDS 25.0%
!! AACAAACATATAGCTTTTGT+TGG - chr5.1:25875499-25875518 MS.gene027568:CDS 25.0%
!! CTGATTTAGGTTATCTTTAT+TGG - chr5.1:25875410-25875429 MS.gene027568:CDS 25.0%
!!! ATTTGAAGGTATTAGAATTG+AGG - chr5.1:25876149-25876168 MS.gene027568:CDS 25.0%
!!! CTAGCAATTTGTTACTATTA+AGG - chr5.1:25875204-25875223 MS.gene027568:CDS 25.0%
AAGAGTAAGTAGATGAATAG+TGG + chr5.1:25875045-25875064 None:intergenic 30.0%
AATGGAAAAGGATTCATGTA+AGG + chr5.1:25876225-25876244 None:intergenic 30.0%
ACATGATTGTTATATCAACC+GGG - chr5.1:25875779-25875798 MS.gene027568:CDS 30.0%
AGAAAAGTTGCTAAAGGAAA+TGG - chr5.1:25875946-25875965 MS.gene027568:intron 30.0%
AGTCCAATAAATTTCGTAAG+TGG - chr5.1:25875608-25875627 MS.gene027568:CDS 30.0%
ATCTCATTCCAAGTATCTAT+AGG + chr5.1:25876306-25876325 None:intergenic 30.0%
ATGTGGTGACATTGTATATA+GGG + chr5.1:25875345-25875364 None:intergenic 30.0%
ATTGAGAGAAAAGTTGCTAA+AGG - chr5.1:25875940-25875959 MS.gene027568:intron 30.0%
GACATGATTGTTATATCAAC+CGG - chr5.1:25875778-25875797 MS.gene027568:CDS 30.0%
GGATGAAGTATAAACAAAGA+AGG + chr5.1:25874961-25874980 None:intergenic 30.0%
GTGTTGAAAGATCAAATGAA+GGG - chr5.1:25875689-25875708 MS.gene027568:CDS 30.0%
GTTTGTTCTTAATGAGTTTG+AGG + chr5.1:25875486-25875505 None:intergenic 30.0%
TACAAAATGATTGCGTTCAT+TGG - chr5.1:25876268-25876287 MS.gene027568:CDS 30.0%
TAGGTTATCTTTATTGGAGA+TGG - chr5.1:25875416-25875435 MS.gene027568:CDS 30.0%
TCACTCTCATAGTAAATTGA+AGG + chr5.1:25875820-25875839 None:intergenic 30.0%
TGACTATTCAAATGGAGATT+GGG - chr5.1:25875303-25875322 MS.gene027568:CDS 30.0%
TGTAGAGATGATGAAGAAAT+GGG - chr5.1:25876329-25876348 MS.gene027568:CDS 30.0%
TGTTACTATTAAGGACTTCT+TGG - chr5.1:25875213-25875232 MS.gene027568:CDS 30.0%
TTACCTACAAAAGAAGTTGA+AGG + chr5.1:25875073-25875092 None:intergenic 30.0%
TTGTAGAGATGATGAAGAAA+TGG - chr5.1:25876328-25876347 MS.gene027568:CDS 30.0%
! AATCCTTTTCCATTTGCAAA+TGG - chr5.1:25876231-25876250 MS.gene027568:CDS 30.0%
! ATCTTCTCCTCTTTTCTATA+AGG + chr5.1:25876054-25876073 None:intergenic 30.0%
! ATGCTACCTTTTCTTTGTAT+TGG - chr5.1:25875647-25875666 MS.gene027568:CDS 30.0%
AAGGATTGAGATAGAAGAAG+TGG - chr5.1:25876101-25876120 MS.gene027568:intron 35.0%
ACAAATGGTAGACCTGATTT+AGG - chr5.1:25875397-25875416 MS.gene027568:CDS 35.0%
ACTCCATTTGCAAATGGAAA+AGG + chr5.1:25876237-25876256 None:intergenic 35.0%
AGGTATTAGAATTGAGGTGT+TGG - chr5.1:25876155-25876174 MS.gene027568:CDS 35.0%
ATATACAATGTCACCACATG+TGG - chr5.1:25875346-25875365 MS.gene027568:CDS 35.0%
ATTTATTGGACTTGCCATCT+TGG + chr5.1:25875600-25875619 None:intergenic 35.0%
CATGTGGTGACATTGTATAT+AGG + chr5.1:25875346-25875365 None:intergenic 35.0%
GAAAAGGTAGCATTATGAGA+AGG + chr5.1:25875640-25875659 None:intergenic 35.0%
GGCAAAGGATATTGATCAAA+TGG - chr5.1:25875756-25875775 MS.gene027568:CDS 35.0%
GTAAGTAGATGAATAGTGGT+AGG + chr5.1:25875041-25875060 None:intergenic 35.0%
GTGACTATTCAAATGGAGAT+TGG - chr5.1:25875302-25875321 MS.gene027568:CDS 35.0%
TGCAAAGAAGTGACTCTAAT+TGG + chr5.1:25875543-25875562 None:intergenic 35.0%
TTCCATTAAGAAAGGCTTTG+AGG - chr5.1:25875899-25875918 MS.gene027568:CDS 35.0%
! AAAGTGTTTGGTTCAAACCT+TGG + chr5.1:25875463-25875482 None:intergenic 35.0%
! AGCTTTTGTTGGAGATTCTT+TGG - chr5.1:25875510-25875529 MS.gene027568:CDS 35.0%
! GACTAAGCCTTATAGAAAAG+AGG - chr5.1:25876044-25876063 MS.gene027568:intron 35.0%
! GGTGTTGAAAGATCAAATGA+AGG - chr5.1:25875688-25875707 MS.gene027568:CDS 35.0%
! GGTGTTGGATGTAACTAAAT+TGG - chr5.1:25876170-25876189 MS.gene027568:CDS 35.0%
! TTTGGAACTCCATTTGCAAA+TGG + chr5.1:25876243-25876262 None:intergenic 35.0%
! TTTTCACCTGCTCATTTTGA+AGG - chr5.1:25875991-25876010 MS.gene027568:intron 35.0%
! TTTTGGATCTTGTCAATGAG+AGG - chr5.1:25875731-25875750 MS.gene027568:CDS 35.0%
!! ATGAGTTTGAGGAAAGTGTT+TGG + chr5.1:25875475-25875494 None:intergenic 35.0%
!! GTGCCACTTACGAAATTTAT+TGG + chr5.1:25875614-25875633 None:intergenic 35.0%
!! TGCTCATTTTGAAGGTGATT+GGG - chr5.1:25875999-25876018 MS.gene027568:intron 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr5.1:25876005-25876024 MS.gene027568:intron 35.0%
!!! ATTTTTTGCAGGGAAGAACA+AGG - chr5.1:25875258-25875277 MS.gene027568:CDS 35.0%
AAAGGATTCATGTAAGGACC+TGG + chr5.1:25876219-25876238 None:intergenic 40.0%
AACACCATGCACTAGAAATG+GGG + chr5.1:25875674-25875693 None:intergenic 40.0%
ACTTGCCATCTTGGTAAACA+AGG + chr5.1:25875591-25875610 None:intergenic 40.0%
AGAAAAGAGGAGAAGATCGT+TGG - chr5.1:25876057-25876076 MS.gene027568:intron 40.0%
AGTAGATGAATAGTGGTAGG+TGG + chr5.1:25875038-25875057 None:intergenic 40.0%
AGTGAGAGATGCATCACAAA+TGG - chr5.1:25875382-25875401 MS.gene027568:CDS 40.0%
AGTTGGTCCAAAAGGAGTAA+GGG + chr5.1:25874990-25875009 None:intergenic 40.0%
CAAAAGGAGTAAGGGACAAA+AGG + chr5.1:25874982-25875001 None:intergenic 40.0%
CAACACCATGCACTAGAAAT+GGG + chr5.1:25875675-25875694 None:intergenic 40.0%
CAATCACCTTCAAAATGAGC+AGG + chr5.1:25876000-25876019 None:intergenic 40.0%
CCATCTTGGTAAACAAGGTT+AGG + chr5.1:25875586-25875605 None:intergenic 40.0%
CCTACAAAAGAAGTTGAAGG+AGG + chr5.1:25875070-25875089 None:intergenic 40.0%
GAATGGATGGTGAGATTAGA+AGG - chr5.1:25876082-25876101 MS.gene027568:intron 40.0%
GAGGGAAGATTGTCATTGAA+GGG + chr5.1:25874887-25874906 None:intergenic 40.0%
GTAGAATGAAGGAGCTTAGT+TGG + chr5.1:25875007-25875026 None:intergenic 40.0%
GTCCTCAAAGCCTTTCTTAA+TGG + chr5.1:25875904-25875923 None:intergenic 40.0%
TAGTTGGTCCAAAAGGAGTA+AGG + chr5.1:25874991-25875010 None:intergenic 40.0%
TCAACACCATGCACTAGAAA+TGG + chr5.1:25875676-25875695 None:intergenic 40.0%
TGGGGACCAATACAAAGAAA+AGG + chr5.1:25875656-25875675 None:intergenic 40.0%
TTCCAAGTATCTATAGGTCC+TGG + chr5.1:25876300-25876319 None:intergenic 40.0%
TTGGCATTATTGAGACCAGA+TGG - chr5.1:25876189-25876208 MS.gene027568:CDS 40.0%
TTGTTATATCAACCGGGCAT+TGG - chr5.1:25875785-25875804 MS.gene027568:CDS 40.0%
TTGTTGGAGATTCTTTGGCT+AGG - chr5.1:25875515-25875534 MS.gene027568:CDS 40.0%
! CCTAACCTTGTTTACCAAGA+TGG - chr5.1:25875583-25875602 MS.gene027568:CDS 40.0%
! CCTCCTTCAACTTCTTTTGT+AGG - chr5.1:25875067-25875086 MS.gene027568:CDS 40.0%
! CTTTTGTCCCTTACTCCTTT+TGG - chr5.1:25874980-25874999 MS.gene027568:CDS 40.0%
!! CTGCTCATTTTGAAGGTGAT+TGG - chr5.1:25875998-25876017 MS.gene027568:intron 40.0%
AAAGAGGAGAAGATCGTTGG+AGG - chr5.1:25876060-25876079 MS.gene027568:intron 45.0%
AAGATCGTTGGAGGAATGGA+TGG - chr5.1:25876069-25876088 MS.gene027568:intron 45.0%
AGGAAGCAAAAACCAATGCC+CGG + chr5.1:25875800-25875819 None:intergenic 45.0%
AGGAGCTTAGTTGGTCCAAA+AGG + chr5.1:25874998-25875017 None:intergenic 45.0%
CAAGTGAGTGTAGTCTACCA+AGG - chr5.1:25875443-25875462 MS.gene027568:CDS 45.0%
CTCTTTGATTGTGCCACATG+TGG + chr5.1:25875362-25875381 None:intergenic 45.0%
CTTGGTAGACTACACTCACT+TGG + chr5.1:25875445-25875464 None:intergenic 45.0%
GGAGGGAAGATTGTCATTGA+AGG + chr5.1:25874888-25874907 None:intergenic 45.0%
GGATCTTGTCAATGAGAGGT+GGG - chr5.1:25875735-25875754 MS.gene027568:CDS 45.0%
TGAATAGTGGTAGGTGGCAA+TGG + chr5.1:25875032-25875051 None:intergenic 45.0%
TGCCAGGACCTATAGATACT+TGG - chr5.1:25876295-25876314 MS.gene027568:CDS 45.0%
TGGATCTTGTCAATGAGAGG+TGG - chr5.1:25875734-25875753 MS.gene027568:CDS 45.0%
TGGCAATGGTGGTAGAATGA+AGG + chr5.1:25875018-25875037 None:intergenic 45.0%
!! GATTGGGTTAGTGACATGAG+AGG - chr5.1:25875319-25875338 MS.gene027568:CDS 45.0%
ATAGTGGTAGGTGGCAATGG+TGG + chr5.1:25875029-25875048 None:intergenic 50.0%
GAAGGTGATTGGGATAAGGC+TGG - chr5.1:25876009-25876028 MS.gene027568:intron 50.0%
GGAGAAGATCGTTGGAGGAA+TGG - chr5.1:25876065-25876084 MS.gene027568:intron 50.0%
TAAGGACCTGGATGACCATC+TGG + chr5.1:25876207-25876226 None:intergenic 50.0%
TGTCAATGAGAGGTGGGCAA+AGG - chr5.1:25875741-25875760 MS.gene027568:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr5.1:25876198-25876217 MS.gene027568:CDS 50.0%
!! TGGTCCCCATTTCTAGTGCA+TGG - chr5.1:25875667-25875686 MS.gene027568:CDS 50.0%
! TGCGTTCATTGGTGCTTGCC+AGG - chr5.1:25876279-25876298 MS.gene027568:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 25874869 25876356 25874869 ID=MS.gene027568
chr5.1 mRNA 25874869 25876356 25874869 ID=MS.gene027568.t1;Parent=MS.gene027568
chr5.1 exon 25876137 25876356 25876137 ID=MS.gene027568.t1.exon1;Parent=MS.gene027568.t1
chr5.1 CDS 25876137 25876356 25876137 ID=cds.MS.gene027568.t1;Parent=MS.gene027568.t1
chr5.1 exon 25874869 25875956 25874869 ID=MS.gene027568.t1.exon2;Parent=MS.gene027568.t1
chr5.1 CDS 25874869 25875956 25874869 ID=cds.MS.gene027568.t1;Parent=MS.gene027568.t1
Gene Sequence

>MS.gene027568

ATGGGAAGCACAAATCCCTTCAATGACAATCTTCCCTCCTTCAATAAGAAATTTTTTCATTTGACTCTTTATTCATTGCTTCCTATATCCCTTCTTTGTTTATACTTCATCCTTTTGTCCCTTACTCCTTTTGGACCAACTAAGCTCCTTCATTCTACCACCATTGCCACCTACCACTATTCATCTACTTACTCTTCTCCTCCTTCAACTTCTTTTGTAGGTAAATATGCACGCATAATTAACTTCTAGTTGAGATTCACATGAACTCTAACTTCTATTCATCTACTTTTTTTTTTAAAAAAAATTATGCTAGTTGAGATTCACATGAACTCTAACTAGCAATTTGTTACTATTAAGGACTTCTTGGTTTACTAATTTTTATGTAATATATTTTTTGCAGGGAAGAACAAGGTTTATGATTATGAGAAATCATGTGACTATTCAAATGGAGATTGGGTTAGTGACATGAGAGGCCCTATATACAATGTCACCACATGTGGCACAATCAAAGAGAGTGAGAGATGCATCACAAATGGTAGACCTGATTTAGGTTATCTTTATTGGAGATGGAAGCCAAGTGAGTGTAGTCTACCAAGGTTTGAACCAAACACTTTCCTCAAACTCATTAAGAACAAACATATAGCTTTTGTTGGAGATTCTTTGGCTAGGAACCAATTAGAGTCACTTCTTTGCATGTTAGCAACTACTTCAACACCTAACCTTGTTTACCAAGATGGCAAGTCCAATAAATTTCGTAAGTGGCACTTTCCTTCTCATAATGCTACCTTTTCTTTGTATTGGTCCCCATTTCTAGTGCATGGTGTTGAAAGATCAAATGAAGGGAATTATTATAATACAATGTTTTTGGATCTTGTCAATGAGAGGTGGGCAAAGGATATTGATCAAATGGACATGATTGTTATATCAACCGGGCATTGGTTTTTGCTTCCTTCAATTTACTATGAGAGTGATTTAATTCTTGGTAGCTTGAATTGTCCCGATTTTAATCATACTCAGATTGATTTTTATGTTCCATTAAGAAAGGCTTTGAGGACTACCCTTAATAGTATAATTGAGAGAAAAGTTGCTAAAGGAAATGGAATTGATGTGATTGTGAAAACATTTTCACCTGCTCATTTTGAAGGTGATTGGGATAAGGCTGGTACTTGTTCAAAGACTAAGCCTTATAGAAAAGAGGAGAAGATCGTTGGAGGAATGGATGGTGAGATTAGAAGGATTGAGATAGAAGAAGTGGAAAGTGCCAAGACAAAAGCTAATAAATTTGAAGGTATTAGAATTGAGGTGTTGGATGTAACTAAATTGGCATTATTGAGACCAGATGGTCATCCAGGTCCTTACATGAATCCTTTTCCATTTGCAAATGGAGTTCCAAAATATGTACAAAATGATTGCGTTCATTGGTGCTTGCCAGGACCTATAGATACTTGGAATGAGATTCTTGTAGAGATGATGAAGAAATGGGAGTAA
Protein sequence

>MS.gene027568.t1

MGSTNPFNDNLPSFNKKFFHLTLYSLLPISLLCLYFILLSLTPFGPTKLLHSTTIATYHYSSTYSSPPSTSFVGKNKVYDYEKSCDYSNGDWVSDMRGPIYNVTTCGTIKESERCITNGRPDLGYLYWRWKPSECSLPRFEPNTFLKLIKNKHIAFVGDSLARNQLESLLCMLATTSTPNLVYQDGKSNKFRKWHFPSHNATFSLYWSPFLVHGVERSNEGNYYNTMFLDLVNERWAKDIDQMDMIVISTGHWFLLPSIYYESDLILGSLNCPDFNHTQIDFYVPLRKALRTTLNSIIERKVAKGNGIDVIVKTFSPAHFEGDWDKAGTCSKTKPYRKEEKIVGGMDGEIRRIEIEEVESAKTKANKFEGIRIEVLDVTKLALLRPDGHPGPYMNPFPFANGVPKYVQNDCVHWCLPGPIDTWNEILVEMMKKWE