Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027577.t1 | XP_003613297.1 | 94.2 | 156 | 9 | 0 | 1 | 156 | 1 | 156 | 3.50E-81 | 310.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027577.t1 | Q93VR4 | 51.7 | 149 | 72 | 0 | 3 | 151 | 1 | 149 | 6.1e-42 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027577.t1 | B7FKA0 | 94.2 | 156 | 9 | 0 | 1 | 156 | 1 | 156 | 2.5e-81 | 310.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027577 | MS.gene022840 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027577.t1 | MTR_5g035010 | 94.231 | 156 | 9 | 0 | 1 | 156 | 1 | 156 | 3.38e-111 | 312 |
| MS.gene027577.t1 | MTR_8g012550 | 29.139 | 151 | 106 | 1 | 1 | 151 | 1 | 150 | 3.90e-19 | 79.0 |
| MS.gene027577.t1 | MTR_1g030810 | 31.373 | 153 | 98 | 5 | 3 | 151 | 1 | 150 | 7.53e-15 | 67.8 |
| MS.gene027577.t1 | MTR_1g030820 | 30.323 | 155 | 97 | 6 | 3 | 151 | 1 | 150 | 1.21e-13 | 64.7 |
| MS.gene027577.t1 | MTR_1g031640 | 29.630 | 135 | 86 | 5 | 9 | 141 | 7 | 134 | 1.62e-12 | 61.6 |
| MS.gene027577.t1 | MTR_1g030840 | 31.852 | 135 | 82 | 4 | 3 | 130 | 1 | 132 | 1.96e-12 | 61.6 |
| MS.gene027577.t1 | MTR_8g045520 | 26.452 | 155 | 110 | 4 | 3 | 155 | 1 | 153 | 1.63e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027577.t1 | AT1G24020 | 51.678 | 149 | 72 | 0 | 3 | 151 | 1 | 149 | 2.65e-55 | 171 |
| MS.gene027577.t1 | AT1G24020 | 51.678 | 149 | 72 | 0 | 3 | 151 | 1 | 149 | 2.65e-55 | 171 |
| MS.gene027577.t1 | AT1G70830 | 34.868 | 152 | 96 | 2 | 2 | 151 | 19 | 169 | 1.39e-22 | 88.6 |
| MS.gene027577.t1 | AT1G70840 | 36.667 | 150 | 92 | 2 | 4 | 151 | 19 | 167 | 2.34e-22 | 87.8 |
| MS.gene027577.t1 | AT1G70830 | 34.868 | 152 | 96 | 2 | 2 | 151 | 19 | 169 | 2.65e-22 | 88.6 |
| MS.gene027577.t1 | AT1G70830 | 34.868 | 152 | 96 | 2 | 2 | 151 | 19 | 169 | 8.98e-22 | 88.2 |
| MS.gene027577.t1 | AT1G70850 | 34.416 | 154 | 98 | 2 | 2 | 153 | 6 | 158 | 1.42e-21 | 87.4 |
| MS.gene027577.t1 | AT1G70830 | 34.868 | 152 | 96 | 2 | 2 | 151 | 19 | 169 | 2.22e-21 | 88.6 |
| MS.gene027577.t1 | AT1G70830 | 33.333 | 150 | 97 | 2 | 4 | 151 | 183 | 331 | 1.30e-19 | 84.0 |
| MS.gene027577.t1 | AT1G70850 | 34.416 | 154 | 98 | 2 | 2 | 153 | 6 | 158 | 4.24e-21 | 87.4 |
| MS.gene027577.t1 | AT1G70850 | 33.813 | 139 | 89 | 2 | 15 | 151 | 175 | 312 | 1.55e-17 | 77.8 |
| MS.gene027577.t1 | AT1G70850 | 34.416 | 154 | 98 | 2 | 2 | 153 | 6 | 158 | 4.24e-21 | 87.4 |
| MS.gene027577.t1 | AT1G70850 | 33.813 | 139 | 89 | 2 | 15 | 151 | 175 | 312 | 1.55e-17 | 77.8 |
| MS.gene027577.t1 | AT1G70890 | 35.526 | 152 | 95 | 3 | 2 | 151 | 4 | 154 | 2.28e-20 | 82.4 |
| MS.gene027577.t1 | AT5G28010 | 32.237 | 152 | 100 | 2 | 4 | 153 | 14 | 164 | 5.22e-20 | 81.6 |
| MS.gene027577.t1 | AT5G28010 | 32.237 | 152 | 100 | 2 | 4 | 153 | 14 | 164 | 5.22e-20 | 81.6 |
| MS.gene027577.t1 | AT1G70830 | 33.333 | 150 | 97 | 2 | 4 | 151 | 136 | 284 | 6.45e-20 | 84.0 |
| MS.gene027577.t1 | AT5G28000 | 31.579 | 152 | 101 | 2 | 4 | 153 | 12 | 162 | 1.55e-17 | 75.1 |
| MS.gene027577.t1 | AT1G70880 | 31.169 | 154 | 103 | 3 | 2 | 153 | 5 | 157 | 3.47e-17 | 74.3 |
| MS.gene027577.t1 | AT1G14930 | 27.815 | 151 | 104 | 3 | 3 | 151 | 1 | 148 | 1.20e-13 | 64.7 |
| MS.gene027577.t1 | AT1G35260 | 31.081 | 148 | 96 | 4 | 9 | 153 | 6 | 150 | 1.59e-13 | 64.3 |
| MS.gene027577.t1 | AT1G23120 | 28.571 | 147 | 91 | 5 | 9 | 154 | 7 | 140 | 1.19e-11 | 59.3 |
| MS.gene027577.t1 | AT1G23120 | 28.571 | 154 | 96 | 5 | 2 | 154 | 50 | 190 | 2.54e-11 | 59.3 |
| MS.gene027577.t1 | AT3G26460 | 25.333 | 150 | 110 | 2 | 3 | 151 | 1 | 149 | 8.11e-11 | 57.4 |
Find 35 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTCATGTGGGAAGGCCTT+AGG | 0.333006 | 5.1:+25809178 | None:intergenic |
| AATAGTTGTAGAATCCCTTA+TGG | 0.338562 | 5.1:+25809204 | None:intergenic |
| GCAAAATCTTTGACAATAGT+TGG | 0.338740 | 5.1:+25808344 | None:intergenic |
| ATCCCCTTCAATTATACAAT+AGG | 0.381348 | 5.1:+25808481 | None:intergenic |
| ATCTCACATCCTTCTCCAAC+TGG | 0.381866 | 5.1:+25808413 | None:intergenic |
| CCTTGAATGATTTGTAGTAT+TGG | 0.436050 | 5.1:+25808453 | None:intergenic |
| CCAATACTACAAATCATTCA+AGG | 0.446219 | 5.1:-25808453 | MS.gene027577:CDS |
| AGTCTAATGCAGACAAGTAC+TGG | 0.454231 | 5.1:-25809231 | MS.gene027577:CDS |
| AGTCATGTGGGAAGGCCTTA+GGG | 0.459268 | 5.1:+25809179 | None:intergenic |
| GAGAAGGATGTGAGATGAAA+TGG | 0.470943 | 5.1:-25808406 | MS.gene027577:CDS |
| ACAAGTACTGGCAAACCATA+AGG | 0.476885 | 5.1:-25809219 | MS.gene027577:CDS |
| GAGGGTGATGGTAAAGCTCC+TGG | 0.482745 | 5.1:-25809136 | MS.gene027577:CDS |
| TCTTTGACAATAGTTGGATC+AGG | 0.487708 | 5.1:+25808350 | None:intergenic |
| GTGAAGTGGCGGATAGAGCC+AGG | 0.488549 | 5.1:+25809118 | None:intergenic |
| GCAACCTATTGTATAATTGA+AGG | 0.505768 | 5.1:-25808485 | MS.gene027577:CDS |
| AAAAGCATTGAGGTCCTTGA+GGG | 0.507266 | 5.1:-25809154 | MS.gene027577:CDS |
| CAAGTACTGGCAAACCATAA+GGG | 0.509772 | 5.1:-25809218 | MS.gene027577:CDS |
| ATCATGGCTGTAGCTCTTCG+TGG | 0.517791 | 5.1:-25809280 | MS.gene027577:CDS |
| CAACCTATTGTATAATTGAA+GGG | 0.524179 | 5.1:-25808484 | MS.gene027577:CDS |
| TACCTTCAGCATAAGTGAAG+TGG | 0.548909 | 5.1:+25809104 | None:intergenic |
| TGCAGTCAAGAACTTCATTG+AGG | 0.557320 | 5.1:-25808324 | MS.gene027577:CDS |
| CAAAAGCATTGAGGTCCTTG+AGG | 0.558857 | 5.1:-25809155 | MS.gene027577:CDS |
| CGCCACTTCACTTATGCTGA+AGG | 0.559188 | 5.1:-25809106 | MS.gene027577:intron |
| ATTGAGGTCCTTGAGGGTGA+TGG | 0.560374 | 5.1:-25809148 | MS.gene027577:CDS |
| CATCTTGCTGTAACACCAGT+TGG | 0.560972 | 5.1:-25808428 | MS.gene027577:CDS |
| CAATACTACAAATCATTCAA+GGG | 0.584423 | 5.1:-25808452 | MS.gene027577:CDS |
| GCTGTAACACCAGTTGGAGA+AGG | 0.585512 | 5.1:-25808422 | MS.gene027577:CDS |
| AACCTATTGTATAATTGAAG+GGG | 0.589185 | 5.1:-25808483 | MS.gene027577:CDS |
| GAGCTTTACCATCACCCTCA+AGG | 0.617751 | 5.1:+25809140 | None:intergenic |
| ATGCTGCTGATGATGAAAAG+AGG | 0.618271 | 5.1:-25808511 | MS.gene027577:CDS |
| AGTCAAGAACTTCATTGAGG+TGG | 0.628959 | 5.1:-25808321 | MS.gene027577:CDS |
| ACAACTCGCGAAGTCTTCGA+CGG | 0.637947 | 5.1:-25808543 | MS.gene027577:CDS |
| CTTCAGCATAAGTGAAGTGG+CGG | 0.638511 | 5.1:+25809107 | None:intergenic |
| AGCTCTTCGTGGAAAGCTAG+AGG | 0.660101 | 5.1:-25809269 | MS.gene027577:CDS |
| ACATGACTACAAAAGCATTG+AGG | 0.674108 | 5.1:-25809164 | MS.gene027577:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATGTAATTCAATAAATA+AGG | - | chr5.1:25808664-25808683 | MS.gene027577:intron | 15.0% |
| !! | TGATGTAATTCAATAAATAA+GGG | - | chr5.1:25808665-25808684 | MS.gene027577:intron | 15.0% |
| !!! | AATAAATAAGGGTATATCAA+TGG | - | chr5.1:25808676-25808695 | MS.gene027577:intron | 20.0% |
| ! | AAAGTGAGTATTATGTATGT+AGG | + | chr5.1:25808593-25808612 | None:intergenic | 25.0% |
| ! | AACCTATTGTATAATTGAAG+GGG | - | chr5.1:25809086-25809105 | MS.gene027577:intron | 25.0% |
| ! | AATTTAGAGACTTTCTTCTT+TGG | - | chr5.1:25808489-25808508 | MS.gene027577:CDS | 25.0% |
| ! | ATAGCATTGTTCAAATTTGT+AGG | + | chr5.1:25808848-25808867 | None:intergenic | 25.0% |
| ! | CAACCTATTGTATAATTGAA+GGG | - | chr5.1:25809085-25809104 | MS.gene027577:intron | 25.0% |
| ! | CAATACTACAAATCATTCAA+GGG | - | chr5.1:25809117-25809136 | MS.gene027577:CDS | 25.0% |
| ! | GATGATTAAAATGAGACATA+GGG | - | chr5.1:25808770-25808789 | MS.gene027577:intron | 25.0% |
| ! | TCATACATTCTTATCTGATT+TGG | - | chr5.1:25808984-25809003 | MS.gene027577:intron | 25.0% |
| !!! | AACCTCCTATTTTGATTTAA+GGG | - | chr5.1:25808868-25808887 | MS.gene027577:intron | 25.0% |
| !!! | TAACCTCCTATTTTGATTTA+AGG | - | chr5.1:25808867-25808886 | MS.gene027577:intron | 25.0% |
| AATAGTTGTAGAATCCCTTA+TGG | + | chr5.1:25808368-25808387 | None:intergenic | 30.0% | |
| AGGCATAGGTCAAATAAAAT+TGG | + | chr5.1:25808828-25808847 | None:intergenic | 30.0% | |
| ATCCCCTTCAATTATACAAT+AGG | + | chr5.1:25809091-25809110 | None:intergenic | 30.0% | |
| CCAATACTACAAATCATTCA+AGG | - | chr5.1:25809116-25809135 | MS.gene027577:CDS | 30.0% | |
| CGATGATTAAAATGAGACAT+AGG | - | chr5.1:25808769-25808788 | MS.gene027577:intron | 30.0% | |
| GCAAAATCTTTGACAATAGT+TGG | + | chr5.1:25809228-25809247 | None:intergenic | 30.0% | |
| GCAACCTATTGTATAATTGA+AGG | - | chr5.1:25809084-25809103 | MS.gene027577:intron | 30.0% | |
| GTGTTCCCTTAAATCAAAAT+AGG | + | chr5.1:25808876-25808895 | None:intergenic | 30.0% | |
| TTCCCTTAAATCAAAATAGG+AGG | + | chr5.1:25808873-25808892 | None:intergenic | 30.0% | |
| TTGTTCAAATTTGTAGGCAT+AGG | + | chr5.1:25808842-25808861 | None:intergenic | 30.0% | |
| ! | CCTTGAATGATTTGTAGTAT+TGG | + | chr5.1:25809119-25809138 | None:intergenic | 30.0% |
| ! | TTCTACAACTATTTTCCCTA+AGG | - | chr5.1:25808375-25808394 | MS.gene027577:CDS | 30.0% |
| !! | TACATTCTTATCTGATTTGG+TGG | - | chr5.1:25808987-25809006 | MS.gene027577:intron | 30.0% |
| AAAATGAGACATAGGGAGTA+TGG | - | chr5.1:25808777-25808796 | MS.gene027577:intron | 35.0% | |
| AAAGCGATACATGAATCTCT+TGG | + | chr5.1:25808918-25808937 | None:intergenic | 35.0% | |
| CTTCTTTGGTAGAGAATGAA+GGG | - | chr5.1:25808503-25808522 | MS.gene027577:CDS | 35.0% | |
| GAGTTGTGATCCTACAATAT+AGG | + | chr5.1:25809013-25809032 | None:intergenic | 35.0% | |
| GTCAAATGCAACGAAAATTG+TGG | + | chr5.1:25808619-25808638 | None:intergenic | 35.0% | |
| TCTTCTTTGGTAGAGAATGA+AGG | - | chr5.1:25808502-25808521 | MS.gene027577:CDS | 35.0% | |
| TCTTTGACAATAGTTGGATC+AGG | + | chr5.1:25809222-25809241 | None:intergenic | 35.0% | |
| ! | CAATGCTTTTGTAGTCATGT+GGG | + | chr5.1:25808405-25808424 | None:intergenic | 35.0% |
| ! | TCAATGCTTTTGTAGTCATG+TGG | + | chr5.1:25808406-25808425 | None:intergenic | 35.0% |
| !! | ACATGACTACAAAAGCATTG+AGG | - | chr5.1:25808405-25808424 | MS.gene027577:CDS | 35.0% |
| !!! | TAGAGAATGAAGGGCATTTT+GGG | - | chr5.1:25808512-25808531 | MS.gene027577:CDS | 35.0% |
| AGTCAAGAACTTCATTGAGG+TGG | - | chr5.1:25809248-25809267 | MS.gene027577:CDS | 40.0% | |
| AGTCTAATGCAGACAAGTAC+TGG | - | chr5.1:25808338-25808357 | MS.gene027577:CDS | 40.0% | |
| GAGAAGGATGTGAGATGAAA+TGG | - | chr5.1:25809163-25809182 | MS.gene027577:CDS | 40.0% | |
| TACCTTCAGCATAAGTGAAG+TGG | + | chr5.1:25808468-25808487 | None:intergenic | 40.0% | |
| TGCAGTCAAGAACTTCATTG+AGG | - | chr5.1:25809245-25809264 | MS.gene027577:CDS | 40.0% | |
| ! | ATGCTGCTGATGATGAAAAG+AGG | - | chr5.1:25809058-25809077 | MS.gene027577:intron | 40.0% |
| !! | AAAAGCATTGAGGTCCTTGA+GGG | - | chr5.1:25808415-25808434 | MS.gene027577:CDS | 40.0% |
| !! | ACAAGTACTGGCAAACCATA+AGG | - | chr5.1:25808350-25808369 | MS.gene027577:CDS | 40.0% |
| !! | CAAGTACTGGCAAACCATAA+GGG | - | chr5.1:25808351-25808370 | MS.gene027577:CDS | 40.0% |
| !! | GATTTGGTGGCCTATATTGT+AGG | - | chr5.1:25809000-25809019 | MS.gene027577:intron | 40.0% |
| !!! | GTAGAGAATGAAGGGCATTT+TGG | - | chr5.1:25808511-25808530 | MS.gene027577:CDS | 40.0% |
| ATCTCACATCCTTCTCCAAC+TGG | + | chr5.1:25809159-25809178 | None:intergenic | 45.0% | |
| CATGAATCTCTTGGAACGCT+AGG | + | chr5.1:25808909-25808928 | None:intergenic | 45.0% | |
| CTTCAGCATAAGTGAAGTGG+CGG | + | chr5.1:25808465-25808484 | None:intergenic | 45.0% | |
| ! | CATCTTGCTGTAACACCAGT+TGG | - | chr5.1:25809141-25809160 | MS.gene027577:CDS | 45.0% |
| ! | GCTTTTGTAGTCATGTGGGA+AGG | + | chr5.1:25808401-25808420 | None:intergenic | 45.0% |
| !! | CAAAAGCATTGAGGTCCTTG+AGG | - | chr5.1:25808414-25808433 | MS.gene027577:CDS | 45.0% |
| ACAACTCGCGAAGTCTTCGA+CGG | - | chr5.1:25809026-25809045 | MS.gene027577:intron | 50.0% | |
| AGCTCTTCGTGGAAAGCTAG+AGG | - | chr5.1:25808300-25808319 | MS.gene027577:CDS | 50.0% | |
| AGTCATGTGGGAAGGCCTTA+GGG | + | chr5.1:25808393-25808412 | None:intergenic | 50.0% | |
| ATTGAGGTCCTTGAGGGTGA+TGG | - | chr5.1:25808421-25808440 | MS.gene027577:CDS | 50.0% | |
| CGCCACTTCACTTATGCTGA+AGG | - | chr5.1:25808463-25808482 | MS.gene027577:CDS | 50.0% | |
| GAGCTTTACCATCACCCTCA+AGG | + | chr5.1:25808432-25808451 | None:intergenic | 50.0% | |
| GCTGTAACACCAGTTGGAGA+AGG | - | chr5.1:25809147-25809166 | MS.gene027577:CDS | 50.0% | |
| TAGTCATGTGGGAAGGCCTT+AGG | + | chr5.1:25808394-25808413 | None:intergenic | 50.0% | |
| ! | GAGGGTGATGGTAAAGCTCC+TGG | - | chr5.1:25808433-25808452 | MS.gene027577:CDS | 55.0% |
| !! | GTGAAGTGGCGGATAGAGCC+AGG | + | chr5.1:25808454-25808473 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 25808292 | 25809299 | 25808292 | ID=MS.gene027577 |
| chr5.1 | mRNA | 25808292 | 25809299 | 25808292 | ID=MS.gene027577.t1;Parent=MS.gene027577 |
| chr5.1 | exon | 25809107 | 25809299 | 25809107 | ID=MS.gene027577.t1.exon1;Parent=MS.gene027577.t1 |
| chr5.1 | CDS | 25809107 | 25809299 | 25809107 | ID=cds.MS.gene027577.t1;Parent=MS.gene027577.t1 |
| chr5.1 | exon | 25808292 | 25808569 | 25808292 | ID=MS.gene027577.t1.exon2;Parent=MS.gene027577.t1 |
| chr5.1 | CDS | 25808292 | 25808569 | 25808292 | ID=cds.MS.gene027577.t1;Parent=MS.gene027577.t1 |
| Gene Sequence |
| Protein sequence |
