AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022840


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022840.t1 MTR_8g012550 96.154 156 6 0 1 156 1 156 1.17e-111 313
MS.gene022840.t1 MTR_8g045570 36.552 145 89 3 8 150 6 149 3.03e-29 105
MS.gene022840.t1 MTR_1g030810 33.974 156 97 4 1 153 1 153 2.49e-27 100
MS.gene022840.t1 MTR_1g030820 35.256 156 95 4 1 153 1 153 3.56e-26 97.1
MS.gene022840.t1 MTR_1g031640 35.664 143 86 3 9 150 7 144 3.73e-25 94.4
MS.gene022840.t1 MTR_8g045490 33.562 146 94 3 8 151 6 150 4.51e-25 94.4
MS.gene022840.t1 MTR_1g030840 35.625 160 94 3 1 153 1 158 1.06e-24 93.6
MS.gene022840.t1 MTR_8g045400 35.172 145 90 4 8 150 6 148 2.20e-24 92.8
MS.gene022840.t1 MTR_8g045695 32.000 150 98 4 8 155 6 153 8.47e-24 91.3
MS.gene022840.t1 MTR_8g045300 31.333 150 100 3 8 155 6 154 1.10e-23 90.9
MS.gene022840.t1 MTR_8g045735 32.667 150 97 4 8 155 6 153 1.77e-23 90.5
MS.gene022840.t1 MTR_8g045665 32.877 146 94 4 8 151 6 149 2.68e-23 89.7
MS.gene022840.t1 MTR_8g045520 32.000 150 99 3 8 155 6 154 5.72e-23 89.0
MS.gene022840.t1 MTR_8g045560 32.192 146 95 4 8 151 6 149 1.38e-22 88.2
MS.gene022840.t1 MTR_8g045555 30.345 145 98 3 8 150 6 149 1.45e-22 87.8
MS.gene022840.t1 MTR_8g045640 31.757 148 97 4 6 151 4 149 2.37e-21 84.7
MS.gene022840.t1 MTR_5g035010 29.139 151 106 1 1 150 1 151 1.84e-17 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022840.t1 AT1G70890 44.516 155 84 1 2 154 4 158 4.74e-46 147
MS.gene022840.t1 AT1G70830 43.871 155 85 1 2 154 19 173 9.74e-44 142
MS.gene022840.t1 AT1G70840 44.516 155 84 1 2 154 17 171 1.43e-43 142
MS.gene022840.t1 AT1G70830 43.312 157 87 1 2 156 19 175 1.72e-43 142
MS.gene022840.t1 AT1G70830 43.312 157 87 1 2 156 19 175 6.46e-43 142
MS.gene022840.t1 AT1G70830 45.161 62 34 0 4 65 183 244 2.16e-14 68.6
MS.gene022840.t1 AT1G70830 43.312 157 87 1 2 156 19 175 5.16e-42 142
MS.gene022840.t1 AT1G70830 42.484 153 86 1 4 154 183 335 4.39e-39 135
MS.gene022840.t1 AT1G70850 43.709 151 83 1 2 150 6 156 2.73e-41 138
MS.gene022840.t1 AT1G70850 46.939 49 26 0 15 63 175 223 4.86e-11 59.3
MS.gene022840.t1 AT1G70850 43.709 151 83 1 2 150 6 156 1.24e-40 139
MS.gene022840.t1 AT1G70850 45.775 142 75 1 15 154 175 316 4.26e-40 137
MS.gene022840.t1 AT1G70850 43.709 151 83 1 2 150 6 156 1.24e-40 139
MS.gene022840.t1 AT1G70850 45.775 142 75 1 15 154 175 316 4.26e-40 137
MS.gene022840.t1 AT1G70830 42.484 153 86 1 4 154 136 288 1.37e-39 135
MS.gene022840.t1 AT1G70830 39.796 98 57 1 61 156 31 128 6.06e-18 78.6
MS.gene022840.t1 AT5G28010 40.260 154 90 1 3 154 13 166 2.63e-39 131
MS.gene022840.t1 AT5G28010 40.260 154 90 1 3 154 13 166 2.63e-39 131
MS.gene022840.t1 AT1G70880 40.645 155 90 1 2 154 5 159 8.35e-39 129
MS.gene022840.t1 AT5G28000 37.584 149 91 1 8 154 16 164 1.61e-32 113
MS.gene022840.t1 AT1G23130 34.459 148 94 2 8 153 13 159 6.00e-29 104
MS.gene022840.t1 AT1G35260 39.073 151 87 3 7 154 4 152 1.72e-27 100
MS.gene022840.t1 AT1G14930 31.126 151 100 3 1 150 1 148 8.09e-20 80.9
MS.gene022840.t1 AT1G35310 34.043 141 89 3 15 153 12 150 2.90e-19 79.3
MS.gene022840.t1 AT1G14940 31.429 140 94 2 12 150 10 148 2.99e-19 79.3
MS.gene022840.t1 AT1G14960 28.571 140 98 2 12 150 10 148 6.09e-19 78.6
MS.gene022840.t1 AT1G24020 28.477 151 105 2 1 150 1 149 9.32e-18 75.5
MS.gene022840.t1 AT1G24020 28.477 151 105 2 1 150 1 149 9.32e-18 75.5
MS.gene022840.t1 AT2G01520 29.139 151 103 3 1 150 1 148 1.56e-17 74.7
MS.gene022840.t1 AT1G30990 28.571 140 97 3 12 150 10 147 1.84e-17 74.7
MS.gene022840.t1 AT4G14060 29.139 151 103 3 1 150 1 148 1.98e-17 74.7
MS.gene022840.t1 AT1G23120 28.788 132 88 3 2 132 50 176 6.01e-17 74.3
MS.gene022840.t1 AT1G14950 29.139 151 103 3 1 150 1 148 7.45e-17 73.2
MS.gene022840.t1 AT1G23120 28.462 130 87 3 4 132 2 126 7.71e-17 73.2
MS.gene022840.t1 AT4G23680 30.827 133 88 3 1 132 1 130 7.99e-17 73.2
MS.gene022840.t1 AT2G01530 30.464 151 101 3 1 150 1 148 2.99e-16 71.6
MS.gene022840.t1 AT4G23670 28.477 151 104 3 1 150 1 148 3.66e-16 71.2
MS.gene022840.t1 AT3G26460 30.714 140 92 4 1 138 1 137 1.79e-15 69.7
MS.gene022840.t1 AT3G26450 29.688 128 87 3 13 138 11 137 5.57e-13 63.2
MS.gene022840.t1 AT1G70860 39.062 64 39 0 9 72 12 75 3.08e-11 56.6

Find 35 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATACCCTTTACTATATC+AGG 0.145016 8.1:+84518777 None:intergenic
TAAAACAAAGAGTGAGGATT+AGG 0.284544 8.1:+84517525 None:intergenic
CAGGGCATACATTTGAAATA+TGG 0.304864 8.1:+84518796 None:intergenic
AGAAATGGATGTGCCTATTA+AGG 0.342860 8.1:-84518862 MS.gene022840:CDS
AGTGTTGGGTGGAGACTTAT+TGG 0.355222 8.1:-84517649 MS.gene022840:CDS
ACCACACTTCCTTCTCTTTG+TGG 0.370613 8.1:+84517588 None:intergenic
TGCTGGGGAGTGGCCTTAAT+AGG 0.384194 8.1:+84518849 None:intergenic
TTGAACTTCATGAAGGTGAT+TGG 0.384755 8.1:-84518758 MS.gene022840:CDS
TGAACTTCATGAAGGTGATT+GGG 0.392703 8.1:-84518757 MS.gene022840:CDS
CAAAATAACTTTCAAAGTGT+TGG 0.400707 8.1:-84517664 MS.gene022840:CDS
AAAATAACTTTCAAAGTGTT+GGG 0.411266 8.1:-84517663 MS.gene022840:CDS
TGCAATTGAAAAGATAGAAA+TGG 0.422798 8.1:-84518877 MS.gene022840:CDS
AAAACAAAGAGTGAGGATTA+GGG 0.456937 8.1:+84517526 None:intergenic
CTTACCGAAAACATAATTCC+AGG 0.459113 8.1:+84518713 None:intergenic
GAAGGTGATTGGGGCACTGA+GGG 0.465665 8.1:-84518747 MS.gene022840:CDS
AAACTTGTGTTTCTAAGCAA+AGG 0.480042 8.1:-84517713 MS.gene022840:CDS
CTGAGGGGTGCATTATATCC+TGG 0.482569 8.1:-84518731 MS.gene022840:CDS
ACAAAACTTCATAAAACTGC+TGG 0.482751 8.1:+84518832 None:intergenic
GTATGCCCTGATATAGTAAA+GGG 0.486941 8.1:-84518783 MS.gene022840:CDS
TTCATAAAACTGCTGGGGAG+TGG 0.515855 8.1:+84518839 None:intergenic
TGTATGCCCTGATATAGTAA+AGG 0.517053 8.1:-84518784 MS.gene022840:CDS
CAAAACTTCATAAAACTGCT+GGG 0.538598 8.1:+84518833 None:intergenic
GTTGGGTGGAGACTTATTGG+AGG 0.547743 8.1:-84517646 MS.gene022840:CDS
CAAATTATGCCACAAAGAGA+AGG 0.567700 8.1:-84517597 MS.gene022840:CDS
CAATACCCTTTACTATATCA+GGG 0.568506 8.1:+84518778 None:intergenic
GAACTTCATGAAGGTGATTG+GGG 0.570247 8.1:-84518756 MS.gene022840:CDS
GAGAAGGAAGTGTGGTTCGT+CGG 0.576383 8.1:-84517581 MS.gene022840:CDS
ATAACTTTCAAAGTGTTGGG+TGG 0.599542 8.1:-84517660 MS.gene022840:CDS
GACAAATAAAACAAAGAGTG+AGG 0.603900 8.1:+84517519 None:intergenic
TGAAGGTGATTGGGGCACTG+AGG 0.615934 8.1:-84518748 MS.gene022840:CDS
TGATGCTCATCTTGCACCCG+AGG 0.618366 8.1:-84517475 MS.gene022840:CDS
AAGGGTATTGAACTTCATGA+AGG 0.622666 8.1:-84518765 MS.gene022840:CDS
AAGGTGATTGGGGCACTGAG+GGG 0.666433 8.1:-84518746 MS.gene022840:CDS
AAAACTTCATAAAACTGCTG+GGG 0.672455 8.1:+84518834 None:intergenic
GCCACAAAGAGAAGGAAGTG+TGG 0.682037 8.1:-84517589 MS.gene022840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTATTAGATTAATATAT+AGG + chr8.1:84518574-84518593 None:intergenic 10.0%
!! TTATGATTATTTATAAGTTA+AGG - chr8.1:84518186-84518205 MS.gene022840:intron 10.0%
!!! TTATTTTTTTATTGAAAACA+CGG + chr8.1:84518274-84518293 None:intergenic 10.0%
!!! AAATTTAGAGTTTGATATAT+TGG - chr8.1:84518215-84518234 MS.gene022840:intron 15.0%
!!! AAATTTTATTTACTTTCGAT+CGG + chr8.1:84518384-84518403 None:intergenic 15.0%
!!! TTACTTTTATCTTAACTTTT+CGG - chr8.1:84517746-84517765 MS.gene022840:intron 15.0%
!! ACAAAAATATATATGCAAGT+TGG + chr8.1:84518318-84518337 None:intergenic 20.0%
!! TAAAAGTAACTTGAGTATAA+AGG + chr8.1:84517735-84517754 None:intergenic 20.0%
!! TGTCTTATCTATAATTTATG+TGG - chr8.1:84517968-84517987 MS.gene022840:intron 20.0%
!!! AAAATAACTTTCAAAGTGTT+GGG - chr8.1:84518689-84518708 MS.gene022840:intron 20.0%
!!! ATTTTTACTTTTAAAGAGCA+TGG - chr8.1:84518108-84518127 MS.gene022840:intron 20.0%
!!! TTTTACAAGTTGATTTTGTA+AGG + chr8.1:84517915-84517934 None:intergenic 20.0%
! CAAAATAACTTTCAAAGTGT+TGG - chr8.1:84518688-84518707 MS.gene022840:intron 25.0%
! TGCAATTGAAAAGATAGAAA+TGG - chr8.1:84517475-84517494 MS.gene022840:CDS 25.0%
! TTATGCAACAAAAAAAAAGC+TGG - chr8.1:84518614-84518633 MS.gene022840:intron 25.0%
! TTCCAACTATAATTCGATAT+TGG - chr8.1:84518416-84518435 MS.gene022840:intron 25.0%
! TTCCAATATCGAATTATAGT+TGG + chr8.1:84518421-84518440 None:intergenic 25.0%
!! ATATCCTGGAATTATGTTTT+CGG - chr8.1:84517635-84517654 MS.gene022840:CDS 25.0%
!! ATATCTGAATATAGTTGATG+TGG - chr8.1:84518540-84518559 MS.gene022840:intron 25.0%
!! TTTCTTTAAGAAAGTAGTGT+TGG + chr8.1:84518342-84518361 None:intergenic 25.0%
!!! TTTGTTTTATTTGTCTGTTG+AGG - chr8.1:84518841-84518860 MS.gene022840:CDS 25.0%
AAAACAAAGAGTGAGGATTA+GGG + chr8.1:84518829-84518848 None:intergenic 30.0%
AAAACTTCATAAAACTGCTG+GGG + chr8.1:84517521-84517540 None:intergenic 30.0%
ACAAAACTTCATAAAACTGC+TGG + chr8.1:84517523-84517542 None:intergenic 30.0%
CAAAACTTCATAAAACTGCT+GGG + chr8.1:84517522-84517541 None:intergenic 30.0%
CAATACCCTTTACTATATCA+GGG + chr8.1:84517577-84517596 None:intergenic 30.0%
GACAAATAAAACAAAGAGTG+AGG + chr8.1:84518836-84518855 None:intergenic 30.0%
TAAAACAAAGAGTGAGGATT+AGG + chr8.1:84518830-84518849 None:intergenic 30.0%
TCAATACCCTTTACTATATC+AGG + chr8.1:84517578-84517597 None:intergenic 30.0%
! AAACTTGTGTTTCTAAGCAA+AGG - chr8.1:84518639-84518658 MS.gene022840:intron 30.0%
! TGTCATGAACTTTAGTTCAT+CGG + chr8.1:84518007-84518026 None:intergenic 30.0%
!! GTAATTTAGAGTTGATACTG+AGG - chr8.1:84517790-84517809 MS.gene022840:intron 30.0%
AGAAATGGATGTGCCTATTA+AGG - chr8.1:84517490-84517509 MS.gene022840:CDS 35.0%
CAAATTATGCCACAAAGAGA+AGG - chr8.1:84518755-84518774 MS.gene022840:CDS 35.0%
CATCAAAATATATGTACCCC+AGG + chr8.1:84518141-84518160 None:intergenic 35.0%
CTTACCGAAAACATAATTCC+AGG + chr8.1:84517642-84517661 None:intergenic 35.0%
GTATGCCCTGATATAGTAAA+GGG - chr8.1:84517569-84517588 MS.gene022840:CDS 35.0%
GTTTGATATATTGGTCCTCT+CGG - chr8.1:84518224-84518243 MS.gene022840:intron 35.0%
TCTCGACAATAACAACGATT+TGG + chr8.1:84518038-84518057 None:intergenic 35.0%
TGAACTTCATGAAGGTGATT+GGG - chr8.1:84517595-84517614 MS.gene022840:CDS 35.0%
TGTATGCCCTGATATAGTAA+AGG - chr8.1:84517568-84517587 MS.gene022840:CDS 35.0%
TTGAACTTCATGAAGGTGAT+TGG - chr8.1:84517594-84517613 MS.gene022840:CDS 35.0%
! CAGGGCATACATTTGAAATA+TGG + chr8.1:84517559-84517578 None:intergenic 35.0%
! CTTAACTTTTCGGTTCTCAA+GGG - chr8.1:84517756-84517775 MS.gene022840:intron 35.0%
! GTCATGAACTTTAGTTCATC+GGG + chr8.1:84518006-84518025 None:intergenic 35.0%
! TCTTAACTTTTCGGTTCTCA+AGG - chr8.1:84517755-84517774 MS.gene022840:intron 35.0%
!! AAGGGTATTGAACTTCATGA+AGG - chr8.1:84517587-84517606 MS.gene022840:CDS 35.0%
!! ATAACTTTCAAAGTGTTGGG+TGG - chr8.1:84518692-84518711 MS.gene022840:intron 35.0%
GAACTTCATGAAGGTGATTG+GGG - chr8.1:84517596-84517615 MS.gene022840:CDS 40.0%
! AAAGAGCATGGTTGAAAACC+TGG - chr8.1:84518120-84518139 MS.gene022840:intron 40.0%
! AAGAGCATGGTTGAAAACCT+GGG - chr8.1:84518121-84518140 MS.gene022840:intron 40.0%
!! GGATTAGTCGATTCTTGGAT+CGG - chr8.1:84518245-84518264 MS.gene022840:intron 40.0%
ACCACACTTCCTTCTCTTTG+TGG + chr8.1:84518767-84518786 None:intergenic 45.0%
AGAGCATGGTTGAAAACCTG+GGG - chr8.1:84518122-84518141 MS.gene022840:intron 45.0%
CAAGAATCGACTAATCCGAG+AGG + chr8.1:84518242-84518261 None:intergenic 45.0%
GTTCTCAAGGGTAAAAGTCG+TGG - chr8.1:84517768-84517787 MS.gene022840:intron 45.0%
TTCATAAAACTGCTGGGGAG+TGG + chr8.1:84517516-84517535 None:intergenic 45.0%
! AGTGTTGGGTGGAGACTTAT+TGG - chr8.1:84518703-84518722 MS.gene022840:intron 45.0%
!! CTCTCGGATTAGTCGATTCT+TGG - chr8.1:84518240-84518259 MS.gene022840:intron 45.0%
!!! AGATATATTTATTTTAATTT+TGG - chr8.1:84518590-84518609 MS.gene022840:intron 5.0%
!!! CTATATTATATTTTTATATT+TGG - chr8.1:84517879-84517898 MS.gene022840:intron 5.0%
!!! GATATATTTATTTTAATTTT+GGG - chr8.1:84518591-84518610 MS.gene022840:intron 5.0%
GCCACAAAGAGAAGGAAGTG+TGG - chr8.1:84518763-84518782 MS.gene022840:CDS 50.0%
GTTGGGTGGAGACTTATTGG+AGG - chr8.1:84518706-84518725 MS.gene022840:intron 50.0%
! CTGAGGGGTGCATTATATCC+TGG - chr8.1:84517621-84517640 MS.gene022840:CDS 50.0%
! GAGAAGGAAGTGTGGTTCGT+CGG - chr8.1:84518771-84518790 MS.gene022840:CDS 50.0%
TGATGCTCATCTTGCACCCG+AGG - chr8.1:84518877-84518896 MS.gene022840:CDS 55.0%
! TGCTGGGGAGTGGCCTTAAT+AGG + chr8.1:84517506-84517525 None:intergenic 55.0%
!! AAGGTGATTGGGGCACTGAG+GGG - chr8.1:84517606-84517625 MS.gene022840:CDS 55.0%
!! GAAGGTGATTGGGGCACTGA+GGG - chr8.1:84517605-84517624 MS.gene022840:CDS 55.0%
!! TGAAGGTGATTGGGGCACTG+AGG - chr8.1:84517604-84517623 MS.gene022840:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 84517464 84518910 84517464 ID=MS.gene022840
chr8.1 mRNA 84517464 84518910 84517464 ID=MS.gene022840.t1;Parent=MS.gene022840
chr8.1 exon 84518718 84518910 84518718 ID=MS.gene022840.t1.exon1;Parent=MS.gene022840.t1
chr8.1 CDS 84518718 84518910 84518718 ID=cds.MS.gene022840.t1;Parent=MS.gene022840.t1
chr8.1 exon 84517464 84517741 84517464 ID=MS.gene022840.t1.exon2;Parent=MS.gene022840.t1
chr8.1 CDS 84517464 84517741 84517464 ID=cds.MS.gene022840.t1;Parent=MS.gene022840.t1
Gene Sequence

>MS.gene022840

ATGGCAGCATCTGCAATTGAAAAGATAGAAATGGATGTGCCTATTAAGGCCACTCCCCAGCAGTTTTATGAAGTTTTGTGCGACAAGACACACCATATTTCAAATGTATGCCCTGATATAGTAAAGGGTATTGAACTTCATGAAGGTGATTGGGGCACTGAGGGGTGCATTATATCCTGGAATTATGTTTTCGGTAAGTAAACTCAACTTTGTTTATTATATTTGAAAGTTGAAACTCAATTTGTTTCAAAAGAACTATATATATCTTCCTTTATACTCAAGTTACTTTTATCTTAACTTTTCGGTTCTCAAGGGTAAAAGTCGTGGTAATTTAGAGTTGATACTGAGGTAAATTTGATCAAAGATTGTTCTAGTCAGATTCAAACTCGTATTTTTAATATCGATTCATCATTAACTATATTATATTTTTATATTTGGTTTAATTCATCCTTACAAAATCAACTTGTAAAATGAGAGATGCATTCATTTATAAAACTCTTACAATGTCTTATCTATAATTTATGTGGAACTTGAGTTTTCCCGATGAACTAAAGTTCATGACAAATTGAGACCAAATCGTTGTTATTGTCGAGATTTTTATTTGAGATATGATTAAACATGCATATCAACTTTGCTTCTCGTGAATTTTTACTTTTAAAGAGCATGGTTGAAAACCTGGGGTACATATATTTTGATGTAAATTAAATCACTTTTGCATAATTTTATGATTATTTATAAGTTAAGGCTAATTAAATTTAGAGTTTGATATATTGGTCCTCTCGGATTAGTCGATTCTTGGATCGGATACCGTGTTTTCAATAAAAAAATAAAAAATAACGTGCTAGCTATTACCAACTTGCATATATATTTTTGTTCCAACACTACTTTCTTAAAGAAAGAGACTGATAATTTTGAGTCCGATCGAAAGTAAATAAAATTTGATTAAGAATTGTTCCAACTATAATTCGATATTGGAATACGTTTAAGTAATCTGTCTTTAACTGAAGTTTATTAATCACTTGAGTCAAACACATATCGACTTGCATATGAGTAGTTTTGTAACATCATCATGAAATATATCTGAATATAGTTGATGTGGCTCATTTTCCTATATATTAATCTAATAAGATATATTTATTTTAATTTTGGGTTATGCAACAAAAAAAAAGCTGGAAAAACTTGTGTTTCTAAGCAAAGGATTGAAGACATAGACAAAGAAAACAACAAAATAACTTTCAAAGTGTTGGGTGGAGACTTATTGGAGGATTACAAGAGCTTCAAGTTCATAATGCAAATTATGCCACAAAGAGAAGGAAGTGTGGTTCGTCGGATAGTAGAATATGAGAAGCTCAATAACAATGTCCCTAATCCTCACTCTTTGTTTTATTTGTCTGTTGAGGTTCTCAAATATGTTGATGCTCATCTTGCACCCGAGGACAAGAAGTAA

Protein sequence

>MS.gene022840.t1

MAASAIEKIEMDVPIKATPQQFYEVLCDKTHHISNVCPDIVKGIELHEGDWGTEGCIISWNYVFAGKTCVSKQRIEDIDKENNKITFKVLGGDLLEDYKSFKFIMQIMPQREGSVVRRIVEYEKLNNNVPNPHSLFYLSVEVLKYVDAHLAPEDKK