AlfalfaGEDB | MS.gene029532

M. sativa cultivar XinJiangDaYe / MS.gene029532

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029532.t1	XP_013442640.1	85	214	32	0	1	214	1	214	1.70E-97	365.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029532.t1	Q9FKK2	52.7	167	76	2	5	168	3	169	6.7e-39	162.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029532.t1	A0A072TSY1	85.0	214	32	0	1	214	1	214	1.2e-97	365.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene029532.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene029532	MS.gene22094	PPI
MS.gene029532	MS.gene068836	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029532.t1	MTR_0121s0080	84.651	215	33	0	1	215	1	215	8.96e-134	375
MS.gene029532.t1	MTR_7g062350	83.854	192	31	0	24	215	36	227	1.83e-116	332
MS.gene029532.t1	MTR_2g030740	73.023	215	58	0	1	215	1	215	1.27e-113	324
MS.gene029532.t1	MTR_1g105920	72.558	215	59	0	1	215	1	215	4.04e-113	323
MS.gene029532.t1	MTR_8g043650	72.093	215	60	0	1	215	1	215	4.03e-112	320
MS.gene029532.t1	MTR_1g012570	69.767	215	65	0	1	215	1	215	2.51e-108	311
MS.gene029532.t1	MTR_1g105910	70.233	215	64	0	1	215	1	215	2.86e-108	311
MS.gene029532.t1	MTR_1g108510	69.585	217	58	1	1	217	1	209	1.09e-106	306
MS.gene029532.t1	MTR_3g052920	68.837	215	67	0	1	215	1	215	4.48e-106	305
MS.gene029532.t1	MTR_2g093190	70.507	217	56	1	1	217	1	209	1.42e-105	303
MS.gene029532.t1	MTR_3g030770	69.585	217	58	1	1	217	1	209	1.97e-105	303
MS.gene029532.t1	MTR_1g108580	69.585	217	58	1	1	217	1	209	1.51e-104	301
MS.gene029532.t1	MTR_1g075600	62.326	215	81	0	1	215	1	215	1.50e-94	276
MS.gene029532.t1	MTR_1g106070	72.376	181	50	0	1	181	1	181	4.10e-94	273
MS.gene029532.t1	MTR_1g077360	61.395	215	83	0	1	215	1	215	1.60e-93	273
MS.gene029532.t1	MTR_2g085250	60.000	215	86	0	1	215	1	215	2.01e-90	266
MS.gene029532.t1	MTR_3g052870	63.721	215	59	2	1	215	1	196	3.01e-90	265
MS.gene029532.t1	MTR_2g085280	59.535	215	87	0	1	215	1	215	1.57e-89	263
MS.gene029532.t1	MTR_5g053390	60.930	215	84	0	1	215	1	215	1.89e-89	263
MS.gene029532.t1	MTR_5g045560	60.930	215	84	0	1	215	1	215	2.06e-89	263
MS.gene029532.t1	MTR_1g105905	64.467	197	70	0	18	214	1	197	5.90e-89	261
MS.gene029532.t1	MTR_5g055100	58.605	215	89	0	1	215	1	215	7.05e-88	259
MS.gene029532.t1	MTR_3g030780	86.364	132	18	0	1	132	1	132	4.19e-79	233
MS.gene029532.t1	MTR_0121s0100	72.727	132	36	0	1	132	1	132	2.91e-63	193
MS.gene029532.t1	MTR_1g075570	55.056	178	66	1	18	195	1	164	1.97e-54	172
MS.gene029532.t1	MTR_3g080940	43.523	193	103	1	9	195	9	201	2.50e-52	167
MS.gene029532.t1	MTR_3g067910	62.000	100	38	0	23	122	4	103	2.74e-38	129
MS.gene029532.t1	MTR_1g108500	72.917	96	5	1	23	118	111	185	5.15e-37	132
MS.gene029532.t1	MTR_8g086290	38.462	169	94	2	5	170	8	169	1.67e-31	113
MS.gene029532.t1	MTR_2g105290	36.196	163	100	1	5	167	8	166	7.33e-31	112
MS.gene029532.t1	MTR_7g028448	42.336	137	67	3	23	159	26	150	1.07e-25	98.6
MS.gene029532.t1	MTR_4g036915	42.336	137	67	3	23	159	26	150	1.07e-25	98.6
MS.gene029532.t1	MTR_3g067915	64.179	67	24	0	60	126	30	96	3.32e-24	92.4
MS.gene029532.t1	MTR_3g067875	75.000	56	14	0	18	73	1	56	2.24e-23	89.4
MS.gene029532.t1	MTR_4g127140	32.597	181	108	3	5	175	8	184	3.39e-22	89.7
MS.gene029532.t1	MTR_1g054265	41.593	113	64	1	9	121	2	112	3.90e-21	86.7
MS.gene029532.t1	MTR_1g047550	46.667	90	39	1	9	89	2	91	1.66e-20	85.1
MS.gene029532.t1	MTR_4g131030	35.938	128	67	2	9	121	2	129	4.27e-20	85.1
MS.gene029532.t1	MTR_8g079502	53.623	69	32	0	9	77	2	70	5.52e-20	84.0
MS.gene029532.t1	MTR_1g063160	42.857	98	54	1	9	106	2	97	2.25e-19	82.0
MS.gene029532.t1	MTR_3g067870	61.905	63	24	0	16	78	83	145	2.64e-19	81.3
MS.gene029532.t1	MTR_8g051580	50.725	69	34	0	9	77	2	70	2.44e-18	79.3
MS.gene029532.t1	MTR_4g094632	39.091	110	65	1	9	118	2	109	3.56e-18	78.6
MS.gene029532.t1	MTR_8g046350	49.275	69	35	0	9	77	2	70	1.09e-17	77.4
MS.gene029532.t1	MTR_4g094638	37.736	106	65	1	9	113	2	107	3.10e-15	71.2
MS.gene029532.t1	MTR_3g005530	45.588	68	37	0	9	76	2	69	5.61e-15	71.2
MS.gene029532.t1	MTR_8g033270	37.963	108	63	2	7	112	2	107	1.38e-14	70.9
MS.gene029532.t1	MTR_3g452380	40.476	84	50	0	3	86	11	94	1.41e-14	70.9
MS.gene029532.t1	MTR_4g093970	48.485	66	34	0	10	75	29	94	1.76e-14	70.5
MS.gene029532.t1	MTR_6g464720	36.000	125	70	5	7	126	2	121	1.77e-14	70.5
MS.gene029532.t1	MTR_8g022970	31.013	158	96	3	8	159	17	167	3.32e-14	68.6
MS.gene029532.t1	MTR_4g102530	42.466	73	42	0	10	82	3	75	5.35e-14	68.6
MS.gene029532.t1	MTR_4g109830	45.070	71	36	2	7	76	2	70	5.55e-14	68.9
MS.gene029532.t1	MTR_1g053070	36.634	101	63	1	9	108	2	102	5.59e-14	68.9
MS.gene029532.t1	MTR_5g066180	44.118	68	38	0	9	76	2	69	7.78e-14	68.2
MS.gene029532.t1	MTR_4g084740	43.421	76	41	2	9	83	2	76	2.17e-13	68.2
MS.gene029532.t1	MTR_0003s0590	37.805	82	51	0	9	90	2	83	2.69e-13	67.4
MS.gene029532.t1	MTR_3g102570	39.216	102	59	3	9	109	2	101	3.69e-13	67.4
MS.gene029532.t1	MTR_8g087860	44.118	68	38	0	9	76	18	85	4.23e-13	66.6
MS.gene029532.t1	MTR_2g017865	44.118	68	38	0	9	76	17	84	7.58e-13	66.2
MS.gene029532.t1	MTR_8g033220	40.476	84	49	1	10	92	3	86	8.54e-13	65.5
MS.gene029532.t1	MTR_2g009890	41.176	68	40	0	9	76	2	69	9.14e-13	66.2
MS.gene029532.t1	MTR_1g101970	42.647	68	39	0	9	76	2	69	9.28e-13	64.7
MS.gene029532.t1	MTR_5g032150	41.176	68	40	0	9	76	2	69	9.47e-13	65.5
MS.gene029532.t1	MTR_6g015975	45.070	71	36	2	7	76	2	70	9.86e-13	65.5
MS.gene029532.t1	MTR_5g032520	41.176	68	40	0	9	76	2	69	1.01e-12	65.5
MS.gene029532.t1	MTR_7g016600	45.070	71	36	2	7	76	2	70	1.02e-12	65.5
MS.gene029532.t1	MTR_3g084980	36.364	110	64	3	7	112	2	109	1.02e-12	65.5
MS.gene029532.t1	MTR_8g033220	40.476	84	49	1	10	92	3	86	1.05e-12	65.5
MS.gene029532.t1	MTR_8g097090	37.255	102	58	3	7	104	2	101	1.13e-12	65.5
MS.gene029532.t1	MTR_8g066260	43.662	71	37	2	7	76	2	70	1.34e-12	65.1
MS.gene029532.t1	MTR_7g075870	43.284	67	38	0	10	76	3	69	2.11e-12	64.3
MS.gene029532.t1	MTR_7g016630	34.579	107	58	4	7	103	2	106	2.67e-12	64.7
MS.gene029532.t1	MTR_2g461710	51.724	58	26	1	7	64	2	57	3.03e-12	60.1
MS.gene029532.t1	MTR_5g031000	41.176	68	40	0	9	76	2	69	6.55e-12	63.2
MS.gene029532.t1	MTR_5g031000	41.176	68	40	0	9	76	2	69	6.91e-12	63.2
MS.gene029532.t1	MTR_5g046790	34.783	115	56	5	9	111	2	109	7.45e-12	62.8
MS.gene029532.t1	MTR_5g046870	40.580	69	40	1	9	76	2	70	2.83e-11	58.9
MS.gene029532.t1	MTR_7g075870	42.647	68	38	1	10	76	3	70	3.10e-11	60.8
MS.gene029532.t1	MTR_5g021270	42.857	70	38	1	9	76	2	71	3.88e-11	60.8
MS.gene029532.t1	MTR_5g021270	42.857	70	38	1	9	76	2	71	4.29e-11	60.8
MS.gene029532.t1	MTR_3g088615	28.346	127	72	2	9	133	2	111	6.10e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene029532.t1	AT5G60440	52.695	167	76	2	5	168	3	169	2.72e-50	166
MS.gene029532.t1	AT2G24840	43.125	160	82	3	9	163	63	218	2.38e-35	126
MS.gene029532.t1	AT1G65360	38.095	189	110	3	5	192	3	185	1.19e-34	123
MS.gene029532.t1	AT1G01530	40.404	198	103	6	4	190	2	195	2.12e-34	123
MS.gene029532.t1	AT4G36590	36.813	182	107	3	5	179	3	183	3.67e-29	109
MS.gene029532.t1	AT3G04100	28.358	201	130	4	2	193	7	202	1.66e-23	94.0
MS.gene029532.t1	AT2G34440	39.823	113	67	1	9	121	2	113	8.54e-21	85.9
MS.gene029532.t1	AT3G66656	37.815	119	70	1	9	123	2	120	1.51e-20	85.5
MS.gene029532.t1	AT1G28460	29.240	171	114	2	5	168	4	174	5.89e-18	78.6
MS.gene029532.t1	AT1G72350	49.315	73	35	1	9	81	43	113	8.67e-18	79.0
MS.gene029532.t1	AT4G09960	37.190	121	70	3	9	126	2	119	7.08e-16	73.9
MS.gene029532.t1	AT2G45650	48.571	70	34	1	7	76	2	69	9.45e-16	73.9
MS.gene029532.t1	AT4G09960	37.600	125	70	4	5	126	26	145	1.05e-15	73.9
MS.gene029532.t1	AT4G09960	37.190	121	70	3	9	126	2	119	1.22e-15	73.6
MS.gene029532.t1	AT4G09960	37.190	121	70	3	9	126	3	120	1.27e-15	73.2
MS.gene029532.t1	AT4G09960	37.190	121	70	3	9	126	12	129	1.44e-15	73.6
MS.gene029532.t1	AT4G09960	37.600	125	70	4	5	126	95	214	2.04e-15	73.9
MS.gene029532.t1	AT1G29962	36.634	101	61	1	5	102	4	104	2.06e-15	72.0
MS.gene029532.t1	AT2G45660	45.588	68	37	0	10	77	3	70	6.75e-15	69.7
MS.gene029532.t1	AT2G45660	45.588	68	37	0	10	77	3	70	1.51e-14	70.1
MS.gene029532.t1	AT4G22950	42.105	76	44	0	10	85	3	78	1.76e-14	68.9
MS.gene029532.t1	AT1G28450	27.586	174	116	3	5	168	4	177	2.19e-14	68.9
MS.gene029532.t1	AT4G18960	36.000	100	54	1	9	108	18	107	4.36e-14	69.3
MS.gene029532.t1	AT4G22950	46.269	67	36	0	10	76	3	69	5.08e-14	68.9
MS.gene029532.t1	AT4G22950	46.269	67	36	0	10	76	3	69	5.08e-14	68.9
MS.gene029532.t1	AT1G22130	36.957	92	57	1	9	99	2	93	6.71e-14	69.7
MS.gene029532.t1	AT1G77980	45.070	71	38	1	9	78	2	72	9.83e-14	69.3
MS.gene029532.t1	AT1G77950	42.105	76	42	2	9	83	2	76	1.10e-13	68.6
MS.gene029532.t1	AT1G77950	42.105	76	42	2	9	83	2	76	1.10e-13	68.6
MS.gene029532.t1	AT1G77950	42.105	76	42	2	9	83	2	76	1.10e-13	68.6
MS.gene029532.t1	AT1G77950	42.105	76	42	2	9	83	2	76	1.10e-13	68.6
MS.gene029532.t1	AT3G57390	45.714	70	36	1	7	76	2	69	1.16e-13	67.8
MS.gene029532.t1	AT5G51870	47.761	67	35	0	10	76	3	69	1.22e-13	67.4
MS.gene029532.t1	AT5G51870	47.761	67	35	0	10	76	3	69	1.25e-13	66.6
MS.gene029532.t1	AT2G42830	44.000	75	40	1	2	76	12	84	1.29e-13	68.2
MS.gene029532.t1	AT2G42830	44.000	75	40	1	2	76	12	84	1.36e-13	68.2
MS.gene029532.t1	AT2G22540	44.118	68	38	0	9	76	2	69	1.46e-13	67.8
MS.gene029532.t1	AT2G22540	44.118	68	38	0	9	76	2	69	1.46e-13	67.8
MS.gene029532.t1	AT3G57230	39.706	68	41	0	9	76	2	69	1.60e-13	65.9
MS.gene029532.t1	AT3G58780	44.000	75	40	1	2	76	12	84	1.87e-13	67.8
MS.gene029532.t1	AT5G51870	47.761	67	35	0	10	76	3	69	2.00e-13	67.0
MS.gene029532.t1	AT3G58780	44.000	75	40	1	2	76	18	90	2.06e-13	67.8
MS.gene029532.t1	AT5G13790	45.588	68	37	0	9	76	2	69	2.21e-13	67.8
MS.gene029532.t1	AT3G57390	45.714	70	36	1	7	76	2	69	2.22e-13	67.8
MS.gene029532.t1	AT5G51870	47.761	67	35	0	10	76	13	79	2.34e-13	67.0
MS.gene029532.t1	AT5G13790	45.588	68	37	0	9	76	2	69	2.34e-13	67.8
MS.gene029532.t1	AT3G58780	44.000	75	40	1	2	76	12	84	2.65e-13	67.8
MS.gene029532.t1	AT5G51870	47.761	67	35	0	10	76	26	92	2.69e-13	66.2
MS.gene029532.t1	AT4G24540	44.118	68	38	0	9	76	2	69	3.06e-13	66.6
MS.gene029532.t1	AT1G17310	50.000	74	35	1	8	81	47	118	3.38e-13	66.6
MS.gene029532.t1	AT1G26310	38.776	98	44	3	7	90	2	97	4.36e-13	66.6
MS.gene029532.t1	AT1G17310	50.000	74	35	1	8	81	58	129	4.86e-13	66.2
MS.gene029532.t1	AT3G30260	43.662	71	37	2	7	76	2	70	5.09e-13	66.6
MS.gene029532.t1	AT4G37940	41.176	68	40	0	9	76	2	69	5.43e-13	66.2
MS.gene029532.t1	AT4G37940	41.176	68	40	0	9	76	2	69	5.70e-13	65.9
MS.gene029532.t1	AT1G69120	45.070	71	36	2	7	76	2	70	5.92e-13	66.2
MS.gene029532.t1	AT5G10140	35.294	102	61	2	9	109	2	99	6.18e-13	64.7
MS.gene029532.t1	AT4G37940	41.176	68	40	0	9	76	2	69	6.47e-13	65.1
MS.gene029532.t1	AT3G57230	39.706	68	41	0	9	76	2	69	7.26e-13	65.9
MS.gene029532.t1	AT3G57230	39.706	68	41	0	9	76	2	69	7.26e-13	65.9
MS.gene029532.t1	AT5G60910	45.070	71	36	2	7	76	2	70	8.29e-13	65.9
MS.gene029532.t1	AT5G10140	35.294	102	61	2	9	109	2	99	9.56e-13	64.7
MS.gene029532.t1	AT5G10140	34.286	105	64	2	9	112	2	102	1.08e-12	64.7
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.23e-12	63.9
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.23e-12	63.9
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.23e-12	63.9
MS.gene029532.t1	AT5G10140	35.294	102	61	2	9	109	2	99	1.24e-12	64.7
MS.gene029532.t1	AT1G69120	45.070	71	36	2	7	76	2	70	1.28e-12	65.5
MS.gene029532.t1	AT5G20240	33.333	129	63	4	9	133	2	111	1.41e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.48e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.48e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.48e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.48e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.48e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	1.78e-12	62.0
MS.gene029532.t1	AT5G20240	33.333	129	63	4	9	133	2	111	1.98e-12	64.3
MS.gene029532.t1	AT5G15800	45.070	71	36	2	7	76	2	70	2.11e-12	64.7
MS.gene029532.t1	AT3G61120	42.647	68	39	0	9	76	2	69	2.21e-12	64.7
MS.gene029532.t1	AT5G51860	44.776	67	37	0	10	76	3	69	3.50e-12	63.5
MS.gene029532.t1	AT5G15800	45.070	71	36	2	7	76	2	70	3.68e-12	64.3
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.03e-12	63.2
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.03e-12	63.2
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.04e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.04e-12	63.5
MS.gene029532.t1	AT5G62165	41.791	67	39	0	10	76	3	69	4.04e-12	63.5
MS.gene029532.t1	AT5G51860	44.776	67	37	0	10	76	3	69	4.36e-12	63.2
MS.gene029532.t1	AT2G22630	39.706	68	41	0	9	76	2	69	7.24e-12	63.2
MS.gene029532.t1	AT2G22630	39.706	68	41	0	9	76	2	69	7.24e-12	63.2
MS.gene029532.t1	AT3G54340	41.429	70	39	1	9	76	2	71	8.66e-12	62.8
MS.gene029532.t1	AT4G11880	42.647	68	38	1	10	76	3	70	1.66e-11	61.2
MS.gene029532.t1	AT4G11880	42.647	68	38	1	10	76	3	70	1.66e-11	61.2
MS.gene029532.t1	AT3G02310	43.662	71	37	2	7	76	2	70	2.21e-11	61.2
MS.gene029532.t1	AT1G24260	36.735	98	56	3	7	100	2	97	2.79e-11	61.6
MS.gene029532.t1	AT4G11880	42.647	68	38	1	10	76	3	70	3.12e-11	61.2
MS.gene029532.t1	AT4G11880	42.647	68	38	1	10	76	3	70	3.12e-11	61.2
MS.gene029532.t1	AT1G47760	37.313	67	42	0	9	75	2	68	3.18e-11	60.5
MS.gene029532.t1	AT1G24260	36.458	96	55	3	7	98	2	95	3.29e-11	61.2
MS.gene029532.t1	AT1G24260	36.458	96	55	3	7	98	2	95	3.74e-11	61.2
MS.gene029532.t1	AT5G65060	40.580	69	40	1	9	76	2	70	9.97e-11	59.3

Find 40 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATTATTCAAGAAGATTT+TGG	0.197704	1.3:+74044902	MS.gene029532:CDS
TGTTGATAGGAGTGCAATTC+AGG	0.259070	1.3:+74044953	MS.gene029532:CDS
AAATCATTACCAACAGAAAA+TGG	0.265409	1.3:-74044996	None:intergenic
TGGTGGGCTTGTCCGATTAA+TGG	0.300675	1.3:+74044861	MS.gene029532:CDS
AGGCGTTCAAGGCTCAATTT+TGG	0.334747	1.3:+74044841	MS.gene029532:CDS
GTTGAGGATTTGGCTGATAA+TGG	0.350346	1.3:-74045037	None:intergenic
GGTGGGCTTGTCCGATTAAT+GGG	0.369852	1.3:+74044862	MS.gene029532:CDS
CCTTGAACGCCTTGCACAAA+AGG	0.373825	1.3:-74044830	None:intergenic
GTCCGATTAATGGGATGAAT+AGG	0.377904	1.3:+74044871	MS.gene029532:CDS
GCCCTATTCATCCCATTAAT+CGG	0.382765	1.3:-74044873	None:intergenic
TCTATTACCGTATCAACATT+TGG	0.384428	1.3:-74044657	None:intergenic
GTAATAGATCGCTATCTCTC+TGG	0.394171	1.3:+74044672	MS.gene029532:CDS
TTGTTGCTCATCATGTTGAT+AGG	0.402553	1.3:+74044940	MS.gene029532:CDS
TCCGATTAATGGGATGAATA+GGG	0.426477	1.3:+74044872	MS.gene029532:CDS
TAATAGATCGCTATCTCTCT+GGG	0.445296	1.3:+74044673	MS.gene029532:CDS
ATAATGGAGAGGGATGTTAG+AGG	0.448969	1.3:-74045021	None:intergenic
ATTAAATGAGGTTGAACATA+GGG	0.483578	1.3:-74045094	None:intergenic
ACCTTTCCTTCCGCTTGACA+TGG	0.495891	1.3:-74044457	None:intergenic
CGGTCATCCAAATGTTGATA+CGG	0.500939	1.3:+74044650	MS.gene029532:CDS
CACTTGAAGGTTCCTCTCGT+TGG	0.501301	1.3:-74044514	None:intergenic
TGTATTCTCACCTAGTGAAA+AGG	0.518205	1.3:+74044617	MS.gene029532:CDS
AGCATTGAGCTCATACACTT+TGG	0.530404	1.3:-74044748	None:intergenic
GCTCATACACTTTGGCGCTA+CGG	0.535806	1.3:-74044740	None:intergenic
GTTGATAGGAGTGCAATTCA+GGG	0.537321	1.3:+74044954	MS.gene029532:CDS
TTGTTGAAGCCAATTAAATG+AGG	0.551491	1.3:-74045106	None:intergenic
AATTAAATGAGGTTGAACAT+AGG	0.556548	1.3:-74045095	None:intergenic
ACTCAACAACACACTGGACG+AGG	0.585246	1.3:+74044782	MS.gene029532:CDS
ACCATGTCAAGCGGAAGGAA+AGG	0.588345	1.3:+74044456	None:intergenic
GTGAGCCTCAATGAATTCCA+TGG	0.607397	1.3:-74044718	None:intergenic
AGCAACATCTACACCACAGA+GGG	0.623582	1.3:-74044589	None:intergenic
TGTACTTCAACCATGTCAAG+CGG	0.628063	1.3:+74044447	None:intergenic
CTTCAACCATGTCAAGCGGA+AGG	0.628927	1.3:+74044451	None:intergenic
TCAAGCGGAAGGAAAGGTCG+TGG	0.632671	1.3:+74044462	MS.gene029532:CDS
GGATTTGGCTGATAATGGAG+AGG	0.633381	1.3:-74045032	None:intergenic
ACCACAAAACAACAGCACCA+TGG	0.659824	1.3:+74044701	MS.gene029532:CDS
CAGCACCATGGAATTCATTG+AGG	0.664382	1.3:+74044713	MS.gene029532:CDS
GATTTGGCTGATAATGGAGA+GGG	0.685158	1.3:-74045031	None:intergenic
GATTCAACTCAACAACACAC+TGG	0.690388	1.3:+74044776	MS.gene029532:CDS
AATAGATCGCTATCTCTCTG+GGG	0.733972	1.3:+74044674	MS.gene029532:CDS
GAGCAACATCTACACCACAG+AGG	0.777707	1.3:-74044590	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGAATTATTCAAGAAGATTT+TGG	+	chr1.3:74044902-74044921	MS.gene029532:CDS	20.0%
!	AAATCATTACCAACAGAAAA+TGG	-	chr1.3:74044999-74045018	None:intergenic	25.0%
!	ATAGGGTTATGAAAAATTTG+TGG	-	chr1.3:74045080-74045099	None:intergenic	25.0%
!!	AAAGAAAATACCTTTTCACT+AGG	-	chr1.3:74044630-74044649	None:intergenic	25.0%
!!	ATTATTCAAGAAGATTTTGG+TGG	+	chr1.3:74044905-74044924	MS.gene029532:CDS	25.0%
!!	GAAAATATCTCATTTTGTTG+AGG	-	chr1.3:74045056-74045075	None:intergenic	25.0%
!!!	AGTGAAAAGGTATTTTCTTT+CGG	+	chr1.3:74044630-74044649	MS.gene029532:CDS	25.0%
!!!	TCTTTTTCATCTCGATATTT+TGG	-	chr1.3:74044491-74044510	None:intergenic	25.0%
	TCTATTACCGTATCAACATT+TGG	-	chr1.3:74044660-74044679	None:intergenic	30.0%
!	ATCTCATTTTGTTGAGGATT+TGG	-	chr1.3:74045050-74045069	None:intergenic	30.0%
!	CAAACTTTTCCATTTTCTGT+TGG	+	chr1.3:74044987-74045006	MS.gene029532:CDS	30.0%
!	TCTTTGAAAAAGTCACTTGA+AGG	-	chr1.3:74044530-74044549	None:intergenic	30.0%
	TAATAGATCGCTATCTCTCT+GGG	+	chr1.3:74044673-74044692	MS.gene029532:CDS	35.0%
	TCCGATTAATGGGATGAATA+GGG	+	chr1.3:74044872-74044891	MS.gene029532:CDS	35.0%
	TGTATTCTCACCTAGTGAAA+AGG	+	chr1.3:74044617-74044636	MS.gene029532:CDS	35.0%
	TTGTTGCTCATCATGTTGAT+AGG	+	chr1.3:74044940-74044959	MS.gene029532:CDS	35.0%
!!!	ACTTTTTCAAAGAGTCGTAG+CGG	+	chr1.3:74044537-74044556	MS.gene029532:CDS	35.0%
	AATAGATCGCTATCTCTCTG+GGG	+	chr1.3:74044674-74044693	MS.gene029532:CDS	40.0%
	CGGTCATCCAAATGTTGATA+CGG	+	chr1.3:74044650-74044669	MS.gene029532:CDS	40.0%
	GATTCAACTCAACAACACAC+TGG	+	chr1.3:74044776-74044795	MS.gene029532:CDS	40.0%
	GCCCTATTCATCCCATTAAT+CGG	-	chr1.3:74044876-74044895	None:intergenic	40.0%
	GTAATAGATCGCTATCTCTC+TGG	+	chr1.3:74044672-74044691	MS.gene029532:CDS	40.0%
	GTCCGATTAATGGGATGAAT+AGG	+	chr1.3:74044871-74044890	MS.gene029532:CDS	40.0%
	GTTGAGGATTTGGCTGATAA+TGG	-	chr1.3:74045040-74045059	None:intergenic	40.0%
	TGAAAAAGATGACCAACGAG+AGG	+	chr1.3:74044502-74044521	MS.gene029532:CDS	40.0%
!	AGCATTGAGCTCATACACTT+TGG	-	chr1.3:74044751-74044770	None:intergenic	40.0%
!	ATAATGGAGAGGGATGTTAG+AGG	-	chr1.3:74045024-74045043	None:intergenic	40.0%
!	GTTGATAGGAGTGCAATTCA+GGG	+	chr1.3:74044954-74044973	MS.gene029532:CDS	40.0%
!	TGTTGATAGGAGTGCAATTC+AGG	+	chr1.3:74044953-74044972	MS.gene029532:CDS	40.0%
!!	GATTTGGCTGATAATGGAGA+GGG	-	chr1.3:74045034-74045053	None:intergenic	40.0%
!!	GTTCAAGGCTCAATTTTGGT+GGG	+	chr1.3:74044845-74044864	MS.gene029532:CDS	40.0%
!!!	CTTTTTCAAAGAGTCGTAGC+GGG	+	chr1.3:74044538-74044557	MS.gene029532:CDS	40.0%
	ACCACAAAACAACAGCACCA+TGG	+	chr1.3:74044701-74044720	MS.gene029532:CDS	45.0%
	AGCAACATCTACACCACAGA+GGG	-	chr1.3:74044592-74044611	None:intergenic	45.0%
	CAGCACCATGGAATTCATTG+AGG	+	chr1.3:74044713-74044732	MS.gene029532:CDS	45.0%
	GTGAGCCTCAATGAATTCCA+TGG	-	chr1.3:74044721-74044740	None:intergenic	45.0%
!	AGGCGTTCAAGGCTCAATTT+TGG	+	chr1.3:74044841-74044860	MS.gene029532:CDS	45.0%
!	CGTTCAAGGCTCAATTTTGG+TGG	+	chr1.3:74044844-74044863	MS.gene029532:CDS	45.0%
!!	GGATTTGGCTGATAATGGAG+AGG	-	chr1.3:74045035-74045054	None:intergenic	45.0%
!!	TGAGTTGAGCCTTTTGTGCA+AGG	+	chr1.3:74044821-74044840	MS.gene029532:CDS	45.0%
!!!	ATGGTGCTGTTGTTTTGTGG+TGG	-	chr1.3:74044702-74044721	None:intergenic	45.0%
!!!	TCCATGGTGCTGTTGTTTTG+TGG	-	chr1.3:74044705-74044724	None:intergenic	45.0%
!!!	TGGTGCTGTTGTTTTGTGGT+GGG	-	chr1.3:74044701-74044720	None:intergenic	45.0%
	ACTCAACAACACACTGGACG+AGG	+	chr1.3:74044782-74044801	MS.gene029532:CDS	50.0%
	CACTTGAAGGTTCCTCTCGT+TGG	-	chr1.3:74044517-74044536	None:intergenic	50.0%
	CCTTGAACGCCTTGCACAAA+AGG	-	chr1.3:74044833-74044852	None:intergenic	50.0%
	CTGGACGAGGAGAAAAAGCA+CGG	+	chr1.3:74044795-74044814	MS.gene029532:CDS	50.0%
	GAGCAACATCTACACCACAG+AGG	-	chr1.3:74044593-74044612	None:intergenic	50.0%
	GCTCATACACTTTGGCGCTA+CGG	-	chr1.3:74044743-74044762	None:intergenic	50.0%
!	AGTGAGTTTTGCACCCTCTG+TGG	+	chr1.3:74044576-74044595	MS.gene029532:CDS	50.0%
!	GGTGGGCTTGTCCGATTAAT+GGG	+	chr1.3:74044862-74044881	MS.gene029532:CDS	50.0%
!!	CCTTTTGTGCAAGGCGTTCA+AGG	+	chr1.3:74044830-74044849	MS.gene029532:CDS	50.0%
!!	TGGTGGGCTTGTCCGATTAA+TGG	+	chr1.3:74044861-74044880	MS.gene029532:CDS	50.0%
	TCAAGCGGAAGGAAAGGTCG+TGG	+	chr1.3:74044462-74044481	MS.gene029532:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	74044459	74045115	74044459	ID=MS.gene029532
chr1.3	mRNA	74044459	74045115	74044459	ID=MS.gene029532.t1;Parent=MS.gene029532
chr1.3	exon	74044459	74045115	74044459	ID=MS.gene029532.t1.exon1;Parent=MS.gene029532.t1
chr1.3	CDS	74044459	74045115	74044459	ID=cds.MS.gene029532.t1;Parent=MS.gene029532.t1

Gene Sequence

>MS.gene029532

ATGTCAAGCGGAAGGAAAGGTCGTGGTCGCCAAAATATCGAGATGAAAAAGATGACCAACGAGAGGAACCTTCAAGTGACTTTTTCAAAGAGTCGTAGCGGGCTCTTCAAGAAATTCAGTGAGTTTTGCACCCTCTGTGGTGTAGATGTTGCTCTCGTTGTATTCTCACCTAGTGAAAAGGTATTTTCTTTCGGTCATCCAAATGTTGATACGGTAATAGATCGCTATCTCTCTGGGGTCCCACCACAAAACAACAGCACCATGGAATTCATTGAGGCTCACCGTAGCGCCAAAGTGTATGAGCTCAATGCTGAGTTGATTCAACTCAACAACACACTGGACGAGGAGAAAAAGCACGGTGATGAGTTGAGCCTTTTGTGCAAGGCGTTCAAGGCTCAATTTTGGTGGGCTTGTCCGATTAATGGGATGAATAGGGCTCAACTTGAATTATTCAAGAAGATTTTGGTGGAGCTTAAGAAACTTGTTGCTCATCATGTTGATAGGAGTGCAATTCAGGGTGCTTCTGCTCAAACTTTTCCATTTTCTGTTGGTAATGATTTATCCTCTAACATCCCTCTCCATTATCAGCCAAATCCTCAACAAAATGAGATATTTTCGCCACAAATTTTTCATAACCCTATGTTCAACCTCATTTAA

Protein sequence

>MS.gene029532.t1

MSSGRKGRGRQNIEMKKMTNERNLQVTFSKSRSGLFKKFSEFCTLCGVDVALVVFSPSEKVFSFGHPNVDTVIDRYLSGVPPQNNSTMEFIEAHRSAKVYELNAELIQLNNTLDEEKKHGDELSLLCKAFKAQFWWACPINGMNRAQLELFKKILVELKKLVAHHVDRSAIQGASAQTFPFSVGNDLSSNIPLHYQPNPQQNEIFSPQIFHNPMFNLI