AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene030435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030435.t1 MTR_8g096990 92.906 437 26 1 1 437 1 432 0.0 823
MS.gene030435.t1 MTR_8g038250 76.298 443 72 3 1 437 1 416 0.0 678
MS.gene030435.t1 MTR_5g080890 75.399 439 101 4 1 437 1 434 0.0 672
MS.gene030435.t1 MTR_5g080960 75.626 439 100 4 1 437 1 434 0.0 671
MS.gene030435.t1 MTR_8g038280 71.854 437 122 1 1 437 1 436 0.0 647
MS.gene030435.t1 MTR_5g044770 71.396 437 119 2 1 437 1 431 0.0 636
MS.gene030435.t1 MTR_6g090290 68.192 437 137 2 1 436 1 436 0.0 580
MS.gene030435.t1 MTR_8g038310 62.700 437 138 4 1 436 1 413 0.0 540
MS.gene030435.t1 MTR_4g109760 66.362 437 109 5 1 437 1 399 0.0 533
MS.gene030435.t1 MTR_3g028120 53.682 421 182 7 25 437 24 439 2.44e-149 432
MS.gene030435.t1 MTR_6g088760 53.846 416 176 8 1 407 1 409 2.34e-146 427
MS.gene030435.t1 MTR_2g067830 52.670 412 182 8 32 436 1 406 2.25e-143 415
MS.gene030435.t1 MTR_5g038360 49.203 439 204 9 8 436 7 436 2.37e-136 399
MS.gene030435.t1 MTR_2g105300 51.345 409 164 9 32 433 1 381 1.00e-131 385
MS.gene030435.t1 MTR_0024s0040 50.718 418 187 9 30 436 1 410 1.98e-127 375
MS.gene030435.t1 MTR_5g044570 47.545 448 186 13 1 436 1 411 2.78e-126 372
MS.gene030435.t1 MTR_0036s0070 43.567 443 226 9 1 436 1 426 6.58e-115 343
MS.gene030435.t1 MTR_0036s0050 43.653 449 226 10 1 436 1 435 2.41e-113 340
MS.gene030435.t1 MTR_0036s0110 41.228 456 234 10 1 436 1 442 4.27e-108 327
MS.gene030435.t1 MTR_5g008070 41.943 422 219 10 21 435 23 425 3.24e-100 306
MS.gene030435.t1 MTR_2g059490 41.324 438 214 11 8 437 9 411 1.04e-95 295
MS.gene030435.t1 MTR_2g059460 41.371 423 198 12 23 436 25 406 8.21e-94 289
MS.gene030435.t1 MTR_5g008150 40.933 386 211 8 59 436 36 412 7.75e-92 284
MS.gene030435.t1 MTR_5g008110 37.868 441 222 11 3 436 20 415 4.33e-88 274
MS.gene030435.t1 MTR_3g097150 37.176 425 236 12 27 437 42 449 4.67e-83 262
MS.gene030435.t1 MTR_1g060750 37.838 407 211 11 40 436 2 376 3.95e-78 247
MS.gene030435.t1 MTR_5g008090 38.284 303 170 8 142 436 21 314 8.56e-61 200
MS.gene030435.t1 MTR_4g045857 39.355 310 147 9 14 316 13 288 1.03e-58 194
MS.gene030435.t1 MTR_1g115210 31.860 430 261 12 21 434 73 486 1.14e-54 189
MS.gene030435.t1 MTR_7g117430 32.553 427 249 15 28 434 77 484 7.17e-54 187
MS.gene030435.t1 MTR_1g039270 34.890 364 204 15 84 434 126 469 7.97e-53 184
MS.gene030435.t1 MTR_6g088765 43.388 242 98 5 162 402 5 208 3.88e-50 170
MS.gene030435.t1 MTR_2g028190 35.342 365 209 13 84 434 100 451 1.26e-46 167
MS.gene030435.t1 MTR_3g046300 31.877 389 240 12 68 437 79 461 1.32e-45 164
MS.gene030435.t1 MTR_6g085010 33.521 355 211 11 86 434 156 491 9.71e-45 162
MS.gene030435.t1 MTR_4g007740 31.592 402 243 13 49 435 94 478 1.22e-44 162
MS.gene030435.t1 MTR_1g111350 30.211 427 244 14 21 434 82 467 6.01e-44 160
MS.gene030435.t1 MTR_5g091130 30.266 413 258 12 47 437 152 556 4.64e-40 151
MS.gene030435.t1 MTR_2g070430 27.941 476 281 17 2 436 3 457 1.17e-39 148
MS.gene030435.t1 MTR_3g053710 31.522 368 217 12 85 434 116 466 4.81e-39 146
MS.gene030435.t1 MTR_2g055840 30.208 384 226 14 78 434 5 373 1.73e-37 140
MS.gene030435.t1 MTR_8g103880 28.272 382 249 12 62 435 92 456 3.66e-37 141
MS.gene030435.t1 MTR_3g068000 28.165 387 216 14 89 434 76 441 3.66e-33 130
MS.gene030435.t1 MTR_7g080230 27.765 443 261 17 30 435 11 431 6.04e-33 130
MS.gene030435.t1 MTR_4g008110 54.545 121 27 1 158 278 45 137 5.99e-30 113
MS.gene030435.t1 MTR_1g078240 27.830 424 246 19 53 435 26 430 3.93e-29 119
MS.gene030435.t1 MTR_3g065160 25.134 374 236 14 88 437 76 429 1.10e-28 117
MS.gene030435.t1 MTR_4g095270 26.014 419 259 18 41 437 37 426 2.68e-28 117
MS.gene030435.t1 MTR_7g105850 27.984 486 269 24 8 436 6 467 2.87e-28 116
MS.gene030435.t1 MTR_4g095270 26.014 419 259 18 41 437 37 426 3.84e-28 117
MS.gene030435.t1 MTR_1g078240 28.000 425 245 20 53 435 26 431 6.31e-28 115
MS.gene030435.t1 MTR_8g094590 26.702 382 225 15 85 437 72 427 1.21e-26 111
MS.gene030435.t1 MTR_5g016260 24.512 461 294 19 3 437 2 434 1.40e-26 112
MS.gene030435.t1 MTR_3g053980 29.477 363 227 10 84 434 140 485 1.60e-26 112
MS.gene030435.t1 MTR_5g077850 26.098 387 220 16 88 437 83 440 1.83e-26 111
MS.gene030435.t1 MTR_0945s0010 42.857 126 68 3 315 437 1 125 1.91e-26 104
MS.gene030435.t1 MTR_7g062360 26.173 405 239 17 72 437 45 428 2.43e-26 110
MS.gene030435.t1 MTR_5g016260 24.512 461 294 19 3 437 2 434 2.50e-26 112
MS.gene030435.t1 MTR_3g096930 26.119 402 241 15 65 437 63 437 3.02e-26 110
MS.gene030435.t1 MTR_6g084710 26.463 393 223 16 80 434 186 550 9.33e-26 110
MS.gene030435.t1 MTR_1g037630 26.995 426 274 14 25 435 26 429 3.39e-25 107
MS.gene030435.t1 MTR_8g094590 28.177 362 206 15 85 422 72 403 4.28e-25 106
MS.gene030435.t1 MTR_4g056420 28.158 380 226 17 85 435 76 437 5.41e-25 107
MS.gene030435.t1 MTR_1g029810 26.893 383 224 18 85 437 56 412 6.90e-25 106
MS.gene030435.t1 MTR_1g029810 26.893 383 224 18 85 437 56 412 6.96e-25 106
MS.gene030435.t1 MTR_7g021510 26.650 394 223 17 82 435 67 434 9.26e-25 106
MS.gene030435.t1 MTR_4g023650 27.317 410 231 20 66 437 42 422 1.27e-24 105
MS.gene030435.t1 MTR_7g102750 27.937 383 223 20 85 435 80 441 3.49e-24 105
MS.gene030435.t1 MTR_2g084550 30.184 381 218 18 85 435 76 438 4.58e-24 104
MS.gene030435.t1 MTR_4g023630 27.206 408 234 19 66 437 42 422 6.01e-24 103
MS.gene030435.t1 MTR_4g100990 27.711 415 222 19 86 437 74 473 9.79e-24 103
MS.gene030435.t1 MTR_3g014290 28.691 359 224 12 87 435 81 417 1.05e-23 102
MS.gene030435.t1 MTR_2g103340 43.220 118 23 1 19 136 32 105 1.32e-23 97.8
MS.gene030435.t1 MTR_8g039540 26.291 426 278 14 25 435 30 434 1.46e-23 102
MS.gene030435.t1 MTR_1g061940 27.195 467 271 25 9 435 8 445 3.63e-23 102
MS.gene030435.t1 MTR_6g084710 28.525 305 175 13 80 369 186 462 5.14e-23 101
MS.gene030435.t1 MTR_1g078240 28.771 358 203 17 53 377 26 364 5.94e-23 100
MS.gene030435.t1 MTR_8g094590 30.916 262 149 9 85 338 72 309 3.67e-22 96.7
MS.gene030435.t1 MTR_7g080230 28.371 356 203 16 110 435 28 361 6.11e-22 97.8
MS.gene030435.t1 MTR_7g102750 28.834 326 184 17 85 383 80 384 2.41e-20 93.2
MS.gene030435.t1 MTR_5g022880 26.343 391 228 16 79 434 96 461 8.90e-20 92.0
MS.gene030435.t1 MTR_5g012490 24.746 493 278 24 9 435 5 470 1.05e-19 91.7
MS.gene030435.t1 MTR_3g087780 24.565 460 264 22 28 437 27 453 1.46e-19 91.3
MS.gene030435.t1 MTR_8g075090 26.525 377 222 16 87 436 113 461 5.07e-19 89.7
MS.gene030435.t1 MTR_8g075090 25.352 426 259 18 40 437 68 462 5.48e-19 89.4
MS.gene030435.t1 MTR_5g077920 23.947 380 237 15 88 436 70 428 8.80e-19 88.6
MS.gene030435.t1 MTR_5g061690 28.852 357 196 20 93 422 108 433 2.82e-18 87.4
MS.gene030435.t1 MTR_8g075090 25.635 394 242 16 40 409 68 434 2.69e-16 81.3
MS.gene030435.t1 MTR_3g006820 23.077 390 250 15 78 437 46 415 1.12e-15 79.0
MS.gene030435.t1 MTR_5g080330 24.802 379 180 17 103 434 2 322 1.34e-15 79.0
MS.gene030435.t1 MTR_3g100500 26.526 426 249 19 40 434 45 437 2.21e-15 78.2
MS.gene030435.t1 MTR_8g075010 24.011 379 233 15 87 437 101 452 3.38e-15 78.2
MS.gene030435.t1 MTR_3g087780 25.680 331 198 16 128 437 8 311 6.87e-14 73.2
MS.gene030435.t1 MTR_3g087780 25.680 331 198 16 128 437 44 347 9.71e-14 73.2
MS.gene030435.t1 MTR_2g037710 27.727 220 126 9 224 435 12 206 2.98e-13 68.9
MS.gene030435.t1 MTR_2g037650 36.937 111 58 4 64 174 99 197 4.19e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene030435.t1 AT1G64830 51.449 414 193 4 26 437 24 431 2.58e-141 411
MS.gene030435.t1 AT5G33340 50.242 414 197 5 27 437 30 437 9.46e-132 387
MS.gene030435.t1 AT2G35615 44.695 443 219 8 14 435 7 444 2.32e-116 348
MS.gene030435.t1 AT1G31450 42.921 445 228 8 9 435 6 442 8.90e-108 326
MS.gene030435.t1 AT2G28010 39.367 442 210 12 3 437 4 394 6.22e-85 265
MS.gene030435.t1 AT2G28040 40.000 420 190 12 26 437 28 393 8.28e-82 257
MS.gene030435.t1 AT2G28040 40.000 420 190 12 26 437 19 384 1.49e-81 256
MS.gene030435.t1 AT2G28030 40.800 375 190 9 69 437 42 390 6.98e-77 244
MS.gene030435.t1 AT2G28220 36.242 447 228 11 1 437 1 400 1.04e-75 242
MS.gene030435.t1 AT2G03200 36.986 438 233 14 25 437 42 461 2.38e-75 243
MS.gene030435.t1 AT2G28225 38.786 379 196 10 69 437 48 400 3.47e-71 230
MS.gene030435.t1 AT4G30030 37.173 382 206 16 70 437 61 422 7.24e-58 196
MS.gene030435.t1 AT1G01300 36.066 366 201 13 84 435 139 485 4.07e-54 187
MS.gene030435.t1 AT3G61820 34.509 397 222 15 55 434 108 483 8.30e-53 184
MS.gene030435.t1 AT3G18490 32.812 384 227 14 62 434 137 500 2.29e-49 175
MS.gene030435.t1 AT3G20015 29.571 443 261 16 20 434 51 470 1.61e-47 170
MS.gene030435.t1 AT3G25700 31.651 436 235 20 39 435 39 450 1.56e-46 167
MS.gene030435.t1 AT1G25510 33.609 363 205 14 84 434 145 483 1.23e-45 165
MS.gene030435.t1 AT5G10760 33.985 409 234 14 31 434 87 464 1.46e-45 164
MS.gene030435.t1 AT5G36260 32.115 383 204 15 85 435 76 434 6.86e-44 160
MS.gene030435.t1 AT2G23945 27.155 464 283 14 8 437 9 451 7.20e-43 157
MS.gene030435.t1 AT5G10770 34.247 365 208 12 84 435 129 474 1.02e-42 157
MS.gene030435.t1 AT4G30040 31.193 436 241 22 28 429 17 427 6.28e-40 148
MS.gene030435.t1 AT1G79720 32.880 368 207 13 87 434 135 482 8.19e-40 149
MS.gene030435.t1 AT3G59080 31.105 389 237 14 72 437 155 535 4.39e-38 145
MS.gene030435.t1 AT2G42980 31.552 393 232 17 72 437 145 527 1.39e-37 144
MS.gene030435.t1 AT1G65240 30.446 381 210 15 85 435 72 427 8.29e-37 140
MS.gene030435.t1 AT3G02740 30.390 385 224 15 74 435 77 440 1.68e-36 140
MS.gene030435.t1 AT3G02740 30.108 372 221 14 87 435 10 365 5.16e-36 137
MS.gene030435.t1 AT2G39710 29.211 380 209 17 89 429 67 425 1.10e-33 131
MS.gene030435.t1 AT3G12700 28.455 369 235 13 85 435 104 461 1.66e-32 128
MS.gene030435.t1 AT3G59080 29.427 384 214 13 72 437 155 499 1.15e-29 121
MS.gene030435.t1 AT1G44130 26.667 405 238 14 67 437 23 402 1.39e-29 119
MS.gene030435.t1 AT5G45120 28.502 414 212 19 87 434 83 478 2.87e-29 119
MS.gene030435.t1 AT4G12920 27.865 384 240 15 61 434 31 387 5.10e-29 117
MS.gene030435.t1 AT5G37540 26.649 379 225 15 88 434 81 438 8.81e-29 117
MS.gene030435.t1 AT5G43100 27.100 369 233 15 84 437 73 420 2.92e-28 117
MS.gene030435.t1 AT3G54400 27.877 391 244 13 62 435 56 425 4.00e-28 115
MS.gene030435.t1 AT5G43100 27.100 369 233 15 84 437 73 420 5.79e-28 117
MS.gene030435.t1 AT1G66180 26.064 376 233 16 88 437 73 429 7.99e-28 115
MS.gene030435.t1 AT4G33490 25.236 424 247 15 54 437 21 414 2.26e-27 113
MS.gene030435.t1 AT5G02190 26.649 379 220 17 96 434 82 442 1.02e-26 112
MS.gene030435.t1 AT3G50050 26.895 409 249 16 55 437 55 439 1.03e-26 113
MS.gene030435.t1 AT1G77480 26.562 384 225 17 85 437 65 422 1.04e-25 108
MS.gene030435.t1 AT1G05840 27.895 380 227 15 85 435 78 439 1.12e-25 109
MS.gene030435.t1 AT1G77480 26.562 384 225 17 85 437 65 422 2.09e-25 108
MS.gene030435.t1 AT1G09750 28.108 370 223 15 85 434 102 448 2.33e-25 108
MS.gene030435.t1 AT2G36670 28.382 377 228 16 85 435 98 458 2.87e-24 105
MS.gene030435.t1 AT2G36670 28.267 375 227 16 87 435 34 392 8.15e-24 103
MS.gene030435.t1 AT2G36670 28.267 375 227 16 87 435 34 392 8.15e-24 103
MS.gene030435.t1 AT2G36670 28.267 375 227 16 87 435 105 463 8.88e-24 103
MS.gene030435.t1 AT5G07030 26.850 473 275 19 2 435 15 455 1.28e-23 103
MS.gene030435.t1 AT5G22850 29.211 380 223 18 85 435 79 441 1.94e-23 102
MS.gene030435.t1 AT4G33490 26.957 345 199 12 54 375 18 332 3.78e-23 101
MS.gene030435.t1 AT1G49050 24.311 399 235 17 80 436 197 570 4.87e-22 99.4
MS.gene030435.t1 AT1G49050 23.720 371 223 14 104 436 49 397 7.43e-22 97.4
MS.gene030435.t1 AT2G17760 27.297 370 213 17 92 434 109 449 6.88e-21 95.5
MS.gene030435.t1 AT1G08210 27.321 377 231 14 85 435 116 475 2.10e-20 94.0
MS.gene030435.t1 AT1G08210 26.525 377 234 14 85 435 130 489 2.14e-20 94.0
MS.gene030435.t1 AT1G08210 27.321 377 231 14 85 435 82 441 2.30e-20 93.6
MS.gene030435.t1 AT3G52500 26.190 462 258 22 40 435 24 468 1.86e-19 90.9
MS.gene030435.t1 AT1G49050 26.224 286 169 13 80 347 197 458 2.19e-17 84.7
MS.gene030435.t1 AT3G42550 25.341 367 202 14 87 435 78 390 8.15e-17 82.4
MS.gene030435.t1 AT3G25700 31.282 195 109 7 39 221 39 220 1.79e-16 80.9
MS.gene030435.t1 AT3G51350 25.223 448 270 19 27 434 30 452 2.97e-16 81.3
MS.gene030435.t1 AT1G08210 25.743 404 230 15 85 435 82 468 4.80e-16 80.5
MS.gene030435.t1 AT3G51330 25.474 369 227 16 92 434 107 453 2.75e-13 72.0
MS.gene030435.t1 AT3G51330 25.474 369 227 16 92 434 107 453 4.15e-13 71.6
MS.gene030435.t1 AT4G16563 26.585 410 210 21 104 437 100 494 5.90e-13 70.9
MS.gene030435.t1 AT4G35880 22.669 472 291 20 7 437 16 454 1.95e-12 69.3

Find 78 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTAATATTTATAATAATT+TGG 0.118502 4.4:-6592398 MS.gene030435:CDS
TTAAAATGCGCTGTTATTAA+AGG 0.136468 4.4:+6592277 None:intergenic
CTTGTCCTGTTTGACTCAAT+TGG 0.193642 4.4:+6592628 None:intergenic
GACAATACAGTGTCGTTTAA+AGG 0.228680 4.4:-6592769 MS.gene030435:CDS
GGAGATGCCGCTGTGGTTTC+CGG 0.233729 4.4:-6592592 MS.gene030435:CDS
AGCCCACCATTTAAGTTATA+TGG 0.255193 4.4:-6593096 MS.gene030435:CDS
TGTGAATATACTATTACTTA+TGG 0.265512 4.4:-6592898 MS.gene030435:CDS
ATGAGATACTCACCATTATC+AGG 0.282119 4.4:+6593135 None:intergenic
ACAATGCCATATAACTTAAA+TGG 0.288561 4.4:+6593090 None:intergenic
TCATTTCCTAAAACTGTGAT+AGG 0.290097 4.4:-6592802 MS.gene030435:CDS
GGGTTTGACTCAAGAGAAAA+TGG 0.300912 4.4:+6592649 None:intergenic
TCAGGGATTACAGTTGATTC+AGG 0.307586 4.4:+6593153 None:intergenic
TGTTAAATAGTAGAAAACTA+TGG 0.332231 4.4:+6592531 None:intergenic
GTGCGGCATTGAAAACCTTT+TGG 0.333127 4.4:+6593229 None:intergenic
AGGACAAGCAAACTCAGTTT+TGG 0.344934 4.4:-6592613 MS.gene030435:CDS
TCTCCGGACGTTGCTATCTT+CGG 0.362127 4.4:-6592175 MS.gene030435:CDS
GGTAACATCATAATTGATTC+AGG 0.364855 4.4:-6592442 MS.gene030435:CDS
AAATTATTATAAATATTAGA+TGG 0.365192 4.4:+6592400 None:intergenic
CTTAGCTCAACAAAATTTGT+TGG 0.370088 4.4:-6592149 MS.gene030435:CDS
ACGGAAACAAAGGTACTAAT+AGG 0.381395 4.4:+6592232 None:intergenic
CAAAGGTTTAATTGTTGACT+TGG 0.381798 4.4:+6592331 None:intergenic
TTGTCCTGTTTGACTCAATT+GGG 0.392107 4.4:+6592629 None:intergenic
ATACAGGTAGTGACATTGTT+TGG 0.394829 4.4:-6593065 MS.gene030435:CDS
AGCTCTGGTGTAGTTGGCCT+TGG 0.403335 4.4:-6592742 MS.gene030435:CDS
GTTTGAACTTAGGAGTGGTT+TGG 0.409015 4.4:+6593010 None:intergenic
ACACAACTTGGATCGTCAAT+TGG 0.440174 4.4:-6592697 MS.gene030435:CDS
GTTAAATAGTAGAAAACTAT+GGG 0.440593 4.4:+6592532 None:intergenic
TGGTGGGCTACCAATTGAAT+AGG 0.445541 4.4:+6593110 None:intergenic
CAAACCCAATTGAGTCAAAC+AGG 0.448065 4.4:-6592633 MS.gene030435:CDS
GGAAAATTATATTTGTCTGA+TGG 0.448347 4.4:+6592298 None:intergenic
TTATATGGCATTGTTGATAC+AGG 0.450868 4.4:-6593081 MS.gene030435:CDS
TCTGGTGTAGTTGGCCTTGG+AGG 0.451277 4.4:-6592739 MS.gene030435:CDS
TCGTTTAAAGGCGCAAGCTC+TGG 0.453771 4.4:-6592757 MS.gene030435:CDS
TCAACTGTAATCCCTGATAA+TGG 0.457041 4.4:-6593147 MS.gene030435:CDS
GAAAATTATATTTGTCTGAT+GGG 0.458444 4.4:+6592299 None:intergenic
GCTCAACAAAATTTGTTGGT+TGG 0.458698 4.4:-6592145 MS.gene030435:CDS
CCTAAAACTGTGATAGGATG+TGG 0.472735 4.4:-6592796 MS.gene030435:CDS
TATCTCATGACCTATTCAAT+TGG 0.476455 4.4:-6593120 MS.gene030435:CDS
TTTAAACGACACTGTATTGT+CGG 0.480807 4.4:+6592771 None:intergenic
ACCTTTGTTTCCGTTGCCGA+TGG 0.490821 4.4:-6592223 MS.gene030435:CDS
GGTGTAGTTGGCCTTGGAGG+TGG 0.498111 4.4:-6592736 MS.gene030435:CDS
GGAATCAGCCGTGGTAGAAT+CGG 0.498390 4.4:-6592377 MS.gene030435:CDS
GGCGCAAGCTCTGGTGTAGT+TGG 0.503563 4.4:-6592748 MS.gene030435:CDS
TGGAGATCATTCACACTCAC+AGG 0.519162 4.4:-6592878 MS.gene030435:CDS
TACACAAAACAAGTACCAAA+AGG 0.522317 4.4:-6593244 MS.gene030435:CDS
ATGCCATATAACTTAAATGG+TGG 0.534621 4.4:+6593093 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.540009 4.4:-6592511 MS.gene030435:CDS
GATAGCAACGTCCGGAGAAG+CGG 0.540235 4.4:+6592180 None:intergenic
ATCTTCAAATGGTGGTGACG+AGG 0.547934 4.4:-6592464 MS.gene030435:CDS
GAATTTGACAGATCTTCAAA+TGG 0.549535 4.4:-6592475 MS.gene030435:CDS
CTTACATTAGAGTCCTCTAC+TGG 0.564370 4.4:-6592835 MS.gene030435:CDS
TGAAATATGACCGCCAGTAG+AGG 0.573661 4.4:+6592822 None:intergenic
TGCCATATAACTTAAATGGT+GGG 0.584004 4.4:+6593094 None:intergenic
TTTCCGAAGATAGCAACGTC+CGG 0.584898 4.4:+6592172 None:intergenic
ACAAACAATGCCATCGGCAA+CGG 0.588745 4.4:+6592213 None:intergenic
TGGTTGTAACATTGTTCACA+AGG 0.591014 4.4:+6593030 None:intergenic
TGATGGTTTGAACTTAGGAG+TGG 0.591810 4.4:+6593005 None:intergenic
CTGATGGGGTACTATAGCAA+AGG 0.595866 4.4:+6592314 None:intergenic
AGCAAAACAAACAATGCCAT+CGG 0.596115 4.4:+6592207 None:intergenic
AAAATTATATTTGTCTGATG+GGG 0.596520 4.4:+6592300 None:intergenic
TGACATATGTTAGATGAACA+TGG 0.598301 4.4:+6592958 None:intergenic
GCCATCGGCAACGGAAACAA+AGG 0.604101 4.4:+6592222 None:intergenic
TTTGACAGATCTTCAAATGG+TGG 0.609014 4.4:-6592472 MS.gene030435:CDS
GTGTCTCTTATAACACAACT+TGG 0.612159 4.4:-6592709 MS.gene030435:CDS
GACATATGTTAGATGAACAT+GGG 0.622071 4.4:+6592959 None:intergenic
TGAGATACTCACCATTATCA+GGG 0.623189 4.4:+6593136 None:intergenic
TCTTCAAATGGTGGTGACGA+GGG 0.624427 4.4:-6592463 MS.gene030435:CDS
AGTTGACACAACATCATCAC+CGG 0.625862 4.4:+6592573 None:intergenic
GAGACACGGGACCACCTCCA+AGG 0.631998 4.4:+6592725 None:intergenic
TAATAATTTGGAATCAGCCG+TGG 0.648028 4.4:-6592386 MS.gene030435:CDS
AAGTTGTGTTATAAGAGACA+CGG 0.651825 4.4:+6592711 None:intergenic
AGTTGTGTTATAAGAGACAC+GGG 0.672510 4.4:+6592712 None:intergenic
ACATTAGAGTCCTCTACTGG+CGG 0.682517 4.4:-6592832 MS.gene030435:CDS
GGAGATCATTCACACTCACA+GGG 0.715223 4.4:-6592877 MS.gene030435:CDS
TTAAATAGTAGAAAACTATG+GGG 0.715758 4.4:+6592533 None:intergenic
ATCATCACCGGAAACCACAG+CGG 0.715905 4.4:+6592585 None:intergenic
GAGTTTCACCGATTCTACCA+CGG 0.727068 4.4:+6592369 None:intergenic
ACGGTTGATAGAACGACGTG+CGG 0.734859 4.4:+6593212 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATATTCACAAAATTTTT+GGG + chr4.4:6592559-6592578 None:intergenic 15.0%
!!! TAGTATATTCACAAAATTTT+TGG + chr4.4:6592560-6592579 None:intergenic 15.0%
!! AAAAAAAAGCAATGAAACAA+AGG + chr4.4:6592128-6592147 None:intergenic 20.0%
!! AAAAAAAGCAATGAAACAAA+GGG + chr4.4:6592127-6592146 None:intergenic 20.0%
!! AAAATTATATTTGTCTGATG+GGG + chr4.4:6593168-6593187 None:intergenic 20.0%
!! AAAGAAAAAAAGGATTAGAA+AGG + chr4.4:6592103-6592122 None:intergenic 20.0%
!! GAAAATTATATTTGTCTGAT+GGG + chr4.4:6593169-6593188 None:intergenic 20.0%
!! GTTAAATAGTAGAAAACTAT+GGG + chr4.4:6592936-6592955 None:intergenic 20.0%
!! TGTGAATATACTATTACTTA+TGG - chr4.4:6592567-6592586 MS.gene030435:CDS 20.0%
!! TGTTAAATAGTAGAAAACTA+TGG + chr4.4:6592937-6592956 None:intergenic 20.0%
!! TTAAATAGTAGAAAACTATG+GGG + chr4.4:6592935-6592954 None:intergenic 20.0%
!!! AGTTTTCTACTATTTAACAT+TGG - chr4.4:6592938-6592957 MS.gene030435:CDS 20.0%
! AACAAAGGGAAAAGAAAAAA+AGG + chr4.4:6592113-6592132 None:intergenic 25.0%
! ACAATGCCATATAACTTAAA+TGG + chr4.4:6592378-6592397 None:intergenic 25.0%
! GGAAAATTATATTTGTCTGA+TGG + chr4.4:6593170-6593189 None:intergenic 25.0%
! TTAAAATGCGCTGTTATTAA+AGG + chr4.4:6593191-6593210 None:intergenic 25.0%
!! TTAATAACAGCGCATTTTAA+AGG - chr4.4:6593191-6593210 MS.gene030435:CDS 25.0%
!! TTTTGTAGAAATGATTAGCA+CGG + chr4.4:6592275-6592294 None:intergenic 25.0%
!!! ATGACACAGTTTTTTGTTTA+AGG + chr4.4:6593352-6593371 None:intergenic 25.0%
!!! TTGTAAGTTGATGATTTTGA+TGG + chr4.4:6592480-6592499 None:intergenic 25.0%
AAGTTGTGTTATAAGAGACA+CGG + chr4.4:6592757-6592776 None:intergenic 30.0%
ATGCCATATAACTTAAATGG+TGG + chr4.4:6592375-6592394 None:intergenic 30.0%
CTTAGCTCAACAAAATTTGT+TGG - chr4.4:6593316-6593335 MS.gene030435:CDS 30.0%
GAATTTGACAGATCTTCAAA+TGG - chr4.4:6592990-6593009 MS.gene030435:CDS 30.0%
GACATATGTTAGATGAACAT+GGG + chr4.4:6592509-6592528 None:intergenic 30.0%
TACACAAAACAAGTACCAAA+AGG - chr4.4:6592221-6592240 MS.gene030435:CDS 30.0%
TATCTCATGACCTATTCAAT+TGG - chr4.4:6592345-6592364 MS.gene030435:CDS 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr4.4:6592663-6592682 MS.gene030435:CDS 30.0%
TGACATATGTTAGATGAACA+TGG + chr4.4:6592510-6592529 None:intergenic 30.0%
TGCCATATAACTTAAATGGT+GGG + chr4.4:6592374-6592393 None:intergenic 30.0%
TTATATGGCATTGTTGATAC+AGG - chr4.4:6592384-6592403 MS.gene030435:CDS 30.0%
TTTAAACGACACTGTATTGT+CGG + chr4.4:6592697-6592716 None:intergenic 30.0%
! CAAAGGTTTAATTGTTGACT+TGG + chr4.4:6593137-6593156 None:intergenic 30.0%
! GGTAACATCATAATTGATTC+AGG - chr4.4:6593023-6593042 MS.gene030435:CDS 30.0%
!! GATTTTGATGGTTTGAACTT+AGG + chr4.4:6592468-6592487 None:intergenic 30.0%
!!! TTTTGGTACTTGTTTTGTGT+AGG + chr4.4:6592222-6592241 None:intergenic 30.0%
AGCAAAACAAACAATGCCAT+CGG + chr4.4:6593261-6593280 None:intergenic 35.0%
AGTTGTGTTATAAGAGACAC+GGG + chr4.4:6592756-6592775 None:intergenic 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr4.4:6592400-6592419 MS.gene030435:CDS 35.0%
ATGAGATACTCACCATTATC+AGG + chr4.4:6592333-6592352 None:intergenic 35.0%
GTGTCTCTTATAACACAACT+TGG - chr4.4:6592756-6592775 MS.gene030435:CDS 35.0%
TCAACTGTAATCCCTGATAA+TGG - chr4.4:6592318-6592337 MS.gene030435:CDS 35.0%
TGAGATACTCACCATTATCA+GGG + chr4.4:6592332-6592351 None:intergenic 35.0%
TGGTTGTAACATTGTTCACA+AGG + chr4.4:6592438-6592457 None:intergenic 35.0%
TTGTCCTGTTTGACTCAATT+GGG + chr4.4:6592839-6592858 None:intergenic 35.0%
TTTGACAGATCTTCAAATGG+TGG - chr4.4:6592993-6593012 MS.gene030435:CDS 35.0%
! AGCCCACCATTTAAGTTATA+TGG - chr4.4:6592369-6592388 MS.gene030435:CDS 35.0%
! TAATAATTTGGAATCAGCCG+TGG - chr4.4:6593079-6593098 MS.gene030435:CDS 35.0%
! TTTTCTCATGCGCTAAACAA+TGG - chr4.4:6592144-6592163 MS.gene030435:CDS 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr4.4:6592954-6592973 MS.gene030435:CDS 35.0%
!! ACGGAAACAAAGGTACTAAT+AGG + chr4.4:6593236-6593255 None:intergenic 35.0%
!! ATGGTGATTTTGAAGAATCG+CGG + chr4.4:6592191-6592210 None:intergenic 35.0%
!! GACAATACAGTGTCGTTTAA+AGG - chr4.4:6592696-6592715 MS.gene030435:CDS 35.0%
!! GCTCAACAAAATTTGTTGGT+TGG - chr4.4:6593320-6593339 MS.gene030435:CDS 35.0%
!!! ATGGGGTCTTTTTTCACTAT+AGG + chr4.4:6592918-6592937 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTAGAA+TGG + chr4.4:6592210-6592229 None:intergenic 35.0%
ACACAACTTGGATCGTCAAT+TGG - chr4.4:6592768-6592787 MS.gene030435:CDS 40.0%
AGTTGACACAACATCATCAC+CGG + chr4.4:6592895-6592914 None:intergenic 40.0%
CAAACCCAATTGAGTCAAAC+AGG - chr4.4:6592832-6592851 MS.gene030435:CDS 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr4.4:6592669-6592688 MS.gene030435:CDS 40.0%
CTTACATTAGAGTCCTCTAC+TGG - chr4.4:6592630-6592649 MS.gene030435:CDS 40.0%
CTTGTCCTGTTTGACTCAAT+TGG + chr4.4:6592840-6592859 None:intergenic 40.0%
GGGTTTGACTCAAGAGAAAA+TGG + chr4.4:6592819-6592838 None:intergenic 40.0%
GTTTGAACTTAGGAGTGGTT+TGG + chr4.4:6592458-6592477 None:intergenic 40.0%
TGATGGTTTGAACTTAGGAG+TGG + chr4.4:6592463-6592482 None:intergenic 40.0%
! AGGACAAGCAAACTCAGTTT+TGG - chr4.4:6592852-6592871 MS.gene030435:CDS 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr4.4:6592672-6592691 None:intergenic 40.0%
! TCAGGGATTACAGTTGATTC+AGG + chr4.4:6592315-6592334 None:intergenic 40.0%
!! AACAGCGCATTTTAAAGGTG+CGG - chr4.4:6593196-6593215 MS.gene030435:CDS 40.0%
!! TTTTGCTTTTACCGCTTCTC+CGG - chr4.4:6593274-6593293 MS.gene030435:CDS 40.0%
!!! GGTACTTGTTTTGTGTAGGT+TGG + chr4.4:6592218-6592237 None:intergenic 40.0%
ACAAACAATGCCATCGGCAA+CGG + chr4.4:6593255-6593274 None:intergenic 45.0%
ACATTAGAGTCCTCTACTGG+CGG - chr4.4:6592633-6592652 MS.gene030435:CDS 45.0%
GGAGATCATTCACACTCACA+GGG - chr4.4:6592588-6592607 MS.gene030435:CDS 45.0%
TGAAATATGACCGCCAGTAG+AGG + chr4.4:6592646-6592665 None:intergenic 45.0%
TGGAGATCATTCACACTCAC+AGG - chr4.4:6592587-6592606 MS.gene030435:CDS 45.0%
TTTCCGAAGATAGCAACGTC+CGG + chr4.4:6593296-6593315 None:intergenic 45.0%
! ATCTTCAAATGGTGGTGACG+AGG - chr4.4:6593001-6593020 MS.gene030435:CDS 45.0%
! CTGATGGGGTACTATAGCAA+AGG + chr4.4:6593154-6593173 None:intergenic 45.0%
! TCTTCAAATGGTGGTGACGA+GGG - chr4.4:6593002-6593021 MS.gene030435:CDS 45.0%
!! GAGTTTCACCGATTCTACCA+CGG + chr4.4:6593099-6593118 None:intergenic 45.0%
!! GTGATTTTGAAGAATCGCGG+TGG + chr4.4:6592188-6592207 None:intergenic 45.0%
!! GTGCGGCATTGAAAACCTTT+TGG + chr4.4:6592239-6592258 None:intergenic 45.0%
!! TGGTGGGCTACCAATTGAAT+AGG + chr4.4:6592358-6592377 None:intergenic 45.0%
!! AAATTATTATAAATATTAGA+TGG + chr4.4:6593068-6593087 None:intergenic 5.0%
!! ATCTAATATTTATAATAATT+TGG - chr4.4:6593067-6593086 MS.gene030435:CDS 5.0%
ATCATCACCGGAAACCACAG+CGG + chr4.4:6592883-6592902 None:intergenic 50.0%
GGAATCAGCCGTGGTAGAAT+CGG - chr4.4:6593088-6593107 MS.gene030435:CDS 50.0%
TCTCCGGACGTTGCTATCTT+CGG - chr4.4:6593290-6593309 MS.gene030435:CDS 50.0%
! ACCTTTGTTTCCGTTGCCGA+TGG - chr4.4:6593242-6593261 MS.gene030435:CDS 50.0%
! TCGTTTAAAGGCGCAAGCTC+TGG - chr4.4:6592708-6592727 MS.gene030435:CDS 50.0%
!! ACGGTTGATAGAACGACGTG+CGG + chr4.4:6592256-6592275 None:intergenic 50.0%
GATAGCAACGTCCGGAGAAG+CGG + chr4.4:6593288-6593307 None:intergenic 55.0%
GCCATCGGCAACGGAAACAA+AGG + chr4.4:6593246-6593265 None:intergenic 55.0%
! AGCTCTGGTGTAGTTGGCCT+TGG - chr4.4:6592723-6592742 MS.gene030435:CDS 55.0%
!! TCTGGTGTAGTTGGCCTTGG+AGG - chr4.4:6592726-6592745 MS.gene030435:CDS 55.0%
!!! CAGTTTTGGAGATGCCGCTG+TGG - chr4.4:6592866-6592885 MS.gene030435:CDS 55.0%
GGAGATGCCGCTGTGGTTTC+CGG - chr4.4:6592873-6592892 MS.gene030435:CDS 60.0%
! GGCGCAAGCTCTGGTGTAGT+TGG - chr4.4:6592717-6592736 MS.gene030435:CDS 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG - chr4.4:6592729-6592748 MS.gene030435:CDS 60.0%
GAGACACGGGACCACCTCCA+AGG + chr4.4:6592743-6592762 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 6592087 6593400 6592087 ID=MS.gene030435
chr4.4 mRNA 6592087 6593400 6592087 ID=MS.gene030435.t1;Parent=MS.gene030435
chr4.4 exon 6592087 6593400 6592087 ID=MS.gene030435.t1.exon1;Parent=MS.gene030435.t1
chr4.4 CDS 6592087 6593400 6592087 ID=cds.MS.gene030435.t1;Parent=MS.gene030435.t1
Gene Sequence

>MS.gene030435

ATGAACGCACTTTCCTTTCTAATCCTTTTTTTCTTTTCCCTTTGTTTCATTGCTTTTTTTTCTCATGCGCTAAACAATGGTTTTAGTGTTGAACTCATCCACCGCGATTCTTCAAAATCACCATTCTACCAACCTACACAAAACAAGTACCAAAAGGTTTTCAATGCCGCACGTCGTTCTATCAACCGTGCTAATCATTTCTACAAAAATTCACTTACAAGTACACCTGAATCAACTGTAATCCCTGATAATGGTGAGTATCTCATGACCTATTCAATTGGTAGCCCACCATTTAAGTTATATGGCATTGTTGATACAGGTAGTGACATTGTTTGGCTTCAATGCGAGCCTTGTGAACAATGTTACAACCAAACCACTCCTAAGTTCAAACCATCAAAATCATCAACTTACAAAAATATCCCATGTTCATCTAACATATGTCAATCTGTGAGAGATATCTCTTGTAATGCCCAAAAATTTTGTGAATATACTATTACTTATGGAGATCATTCACACTCACAGGGAGATCTTAGTGTTGATACTCTTACATTAGAGTCCTCTACTGGCGGTCATATTTCATTTCCTAAAACTGTGATAGGATGTGGAACCGACAATACAGTGTCGTTTAAAGGCGCAAGCTCTGGTGTAGTTGGCCTTGGAGGTGGTCCCGTGTCTCTTATAACACAACTTGGATCGTCAATTGGTTCAAAATTCTCTTATTGTTTGCTTCCATTTTCTCTTGAGTCAAACCCAATTGAGTCAAACAGGACAAGCAAACTCAGTTTTGGAGATGCCGCTGTGGTTTCCGGTGATGATGTTGTGTCAACTCCTATAGTGAAAAAAGACCCCATAGTTTTCTACTATTTAACATTGGAAGCATTTAGTGTTGGAAACAAAAGAATAGAATTTGACAGATCTTCAAATGGTGGTGACGAGGGTAACATCATAATTGATTCAGGTACAACATTGACGATTTTGCCATCTAATATTTATAATAATTTGGAATCAGCCGTGGTAGAATCGGTGAAACTCGAGCGTGTTGATGATCCAAGTCAACAATTAAACCTTTGCTATAGTACCCCATCAGACAAATATAATTTTCCTTTAATAACAGCGCATTTTAAAGGTGCGGATATTAAGTTGCATCCTATTAGTACCTTTGTTTCCGTTGCCGATGGCATTGTTTGTTTTGCTTTTACCGCTTCTCCGGACGTTGCTATCTTCGGAAACTTAGCTCAACAAAATTTGTTGGTTGGTTATGACCTTAAACAAAAAACTGTGTCATTTAAGTCTACTGATTGTAGCAAAGTGTAA
Protein sequence

>MS.gene030435.t1

MNALSFLILFFFSLCFIAFFSHALNNGFSVELIHRDSSKSPFYQPTQNKYQKVFNAARRSINRANHFYKNSLTSTPESTVIPDNGEYLMTYSIGSPPFKLYGIVDTGSDIVWLQCEPCEQCYNQTTPKFKPSKSSTYKNIPCSSNICQSVRDISCNAQKFCEYTITYGDHSHSQGDLSVDTLTLESSTGGHISFPKTVIGCGTDNTVSFKGASSGVVGLGGGPVSLITQLGSSIGSKFSYCLLPFSLESNPIESNRTSKLSFGDAAVVSGDDVVSTPIVKKDPIVFYYLTLEAFSVGNKRIEFDRSSNGGDEGNIIIDSGTTLTILPSNIYNNLESAVVESVKLERVDDPSQQLNLCYSTPSDKYNFPLITAHFKGADIKLHPISTFVSVADGIVCFAFTASPDVAIFGNLAQQNLLVGYDLKQKTVSFKSTDCSKV