AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031492

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031492.t1 MTR_4g081950 98.387 248 4 0 1 248 37 284 0.0 502
MS.gene031492.t1 MTR_5g041700 85.830 247 35 0 1 247 1 247 9.38e-163 450
MS.gene031492.t1 MTR_3g094040 79.752 242 49 0 6 247 12 253 1.44e-145 407
MS.gene031492.t1 MTR_3g094060 79.752 242 49 0 6 247 12 253 2.39e-145 406
MS.gene031492.t1 MTR_3g435430 81.624 234 43 0 14 247 14 247 8.92e-144 402
MS.gene031492.t1 MTR_5g075320 76.735 245 56 1 4 247 7 251 1.12e-138 389
MS.gene031492.t1 MTR_3g466790 76.518 247 58 0 1 247 8 254 4.45e-135 380
MS.gene031492.t1 MTR_3g466760 75.806 248 58 2 1 247 14 260 3.14e-133 376
MS.gene031492.t1 MTR_7g011060 74.297 249 53 3 1 247 1 240 7.04e-132 372
MS.gene031492.t1 MTR_5g079950 70.325 246 70 2 1 244 10 254 2.92e-127 361
MS.gene031492.t1 MTR_7g061720 67.984 253 72 3 1 244 1 253 9.68e-123 349
MS.gene031492.t1 MTR_2g097030 68.651 252 69 4 1 244 6 255 2.60e-122 348
MS.gene031492.t1 MTR_6g012520 74.104 251 54 3 1 248 1 243 4.26e-122 347
MS.gene031492.t1 MTR_1g026020 63.563 247 85 4 1 243 5 250 2.17e-114 328
MS.gene031492.t1 MTR_4g063975 66.000 250 78 3 1 244 5 253 9.75e-114 327
MS.gene031492.t1 MTR_1g085460 64.069 231 79 3 16 242 21 251 2.04e-106 308
MS.gene031492.t1 MTR_7g111010 60.000 245 89 4 7 242 4 248 1.42e-103 301
MS.gene031492.t1 MTR_7g110990 61.250 240 79 4 7 242 4 233 6.51e-103 298
MS.gene031492.t1 MTR_4g124340 57.778 225 90 3 23 244 59 281 6.73e-97 285
MS.gene031492.t1 MTR_7g013120 55.731 253 103 4 1 246 1 251 4.79e-95 279
MS.gene031492.t1 MTR_7g013150 59.091 220 86 2 25 242 30 247 1.19e-93 276
MS.gene031492.t1 MTR_8g058990 57.399 223 91 2 25 245 30 250 1.50e-91 270
MS.gene031492.t1 MTR_7g013140 58.108 222 89 2 25 244 28 247 3.15e-91 269
MS.gene031492.t1 MTR_5g076950 55.804 224 98 1 25 247 41 264 1.80e-89 265
MS.gene031492.t1 MTR_8g464350 56.364 220 92 2 25 242 31 248 7.66e-88 261
MS.gene031492.t1 MTR_5g011210 54.955 222 96 3 25 243 37 257 8.08e-86 256
MS.gene031492.t1 MTR_0040s0140 52.444 225 105 1 25 247 32 256 7.73e-85 253
MS.gene031492.t1 MTR_4g102450 55.157 223 94 4 25 243 36 256 1.44e-83 250
MS.gene031492.t1 MTR_2g090255 51.786 224 106 1 25 246 28 251 2.68e-83 249
MS.gene031492.t1 MTR_3g064510 52.155 232 102 5 25 247 53 284 1.65e-77 236
MS.gene031492.t1 MTR_0008s0120 43.439 221 69 3 25 245 32 196 4.41e-52 168
MS.gene031492.t1 MTR_6g012500 76.238 101 24 0 148 248 181 281 1.07e-50 167
MS.gene031492.t1 MTR_2g090270 30.769 234 132 9 25 243 35 253 3.54e-26 102
MS.gene031492.t1 MTR_2g090265 31.197 234 131 10 25 243 33 251 5.65e-25 99.8
MS.gene031492.t1 MTR_0122s0020 31.602 231 134 9 25 243 41 259 1.17e-23 96.3
MS.gene031492.t1 MTR_0118s0070 31.878 229 128 10 30 242 53 269 1.22e-23 96.7
MS.gene031492.t1 MTR_4g028640 28.807 243 140 11 16 242 33 258 6.37e-20 86.3
MS.gene031492.t1 MTR_0809s0010 29.114 237 130 12 25 242 47 264 1.71e-19 85.1
MS.gene031492.t1 MTR_4g099370 26.939 245 162 9 6 242 9 244 2.57e-17 79.0
MS.gene031492.t1 MTR_5g013440 26.033 242 164 8 8 242 12 245 7.74e-16 75.1
MS.gene031492.t1 MTR_4g075790 28.700 223 126 8 36 242 32 237 2.92e-15 73.6
MS.gene031492.t1 MTR_1g085365 58.824 51 21 0 192 242 4 54 7.83e-15 67.4
MS.gene031492.t1 MTR_4g098930 29.046 241 136 12 25 247 29 252 1.14e-14 71.6
MS.gene031492.t1 MTR_0122s0020 31.250 176 98 8 25 189 41 204 5.51e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031492.t1 AT1G69530 81.928 249 43 2 1 247 1 249 3.03e-150 419
MS.gene031492.t1 AT1G69530 81.928 249 43 2 1 247 1 249 3.03e-150 419
MS.gene031492.t1 AT2G03090 78.543 247 53 0 1 247 6 252 2.10e-148 414
MS.gene031492.t1 AT1G69530 82.041 245 42 2 1 243 1 245 3.29e-148 415
MS.gene031492.t1 AT1G26770 81.452 248 45 1 1 247 1 248 1.38e-140 394
MS.gene031492.t1 AT1G26770 81.452 248 45 1 1 247 11 258 2.10e-140 394
MS.gene031492.t1 AT5G56320 72.112 251 66 1 1 247 1 251 4.33e-135 380
MS.gene031492.t1 AT5G56320 72.112 251 66 1 1 247 1 251 4.33e-135 380
MS.gene031492.t1 AT2G40610 74.074 243 62 1 6 247 11 253 1.21e-134 379
MS.gene031492.t1 AT5G56320 72.112 251 66 1 1 247 1 251 2.19e-134 381
MS.gene031492.t1 AT2G39700 69.048 252 70 2 1 244 1 252 2.49e-126 358
MS.gene031492.t1 AT5G05290 69.076 249 74 2 2 247 7 255 4.63e-126 358
MS.gene031492.t1 AT1G69530 71.888 249 38 3 1 247 1 219 2.44e-125 355
MS.gene031492.t1 AT1G69530 73.333 240 52 4 1 238 1 230 6.00e-125 355
MS.gene031492.t1 AT5G02260 67.339 248 77 2 1 244 6 253 4.89e-124 353
MS.gene031492.t1 AT2G37640 70.124 241 69 2 6 244 18 257 5.68e-124 353
MS.gene031492.t1 AT3G55500 72.889 225 59 1 22 244 31 255 1.67e-123 352
MS.gene031492.t1 AT3G29030 73.661 224 50 2 25 247 38 253 7.23e-119 340
MS.gene031492.t1 AT2G28950 66.270 252 77 2 1 244 1 252 1.93e-116 333
MS.gene031492.t1 AT1G20190 70.852 223 62 2 24 243 25 247 7.44e-114 327
MS.gene031492.t1 AT2G37640 71.220 205 57 1 42 244 28 232 3.30e-109 315
MS.gene031492.t1 AT4G01630 62.343 239 86 3 8 242 11 249 3.48e-106 307
MS.gene031492.t1 AT5G39280 55.649 239 101 2 9 244 35 271 1.89e-97 286
MS.gene031492.t1 AT5G39300 58.182 220 90 1 25 244 55 272 1.67e-96 284
MS.gene031492.t1 AT5G39270 57.727 220 91 1 25 244 58 275 1.00e-92 274
MS.gene031492.t1 AT5G39290 56.818 220 93 1 25 244 58 275 5.20e-92 272
MS.gene031492.t1 AT1G20190 67.914 187 57 2 60 243 2 188 9.08e-91 266
MS.gene031492.t1 AT5G39310 56.757 222 93 2 24 244 89 308 1.31e-88 265
MS.gene031492.t1 AT1G12560 54.783 230 101 3 15 242 27 255 1.25e-85 256
MS.gene031492.t1 AT1G62980 52.066 242 110 5 6 242 10 250 1.53e-84 253
MS.gene031492.t1 AT3G03220 52.232 224 106 1 25 247 40 263 1.75e-83 250
MS.gene031492.t1 AT3G15370 52.263 243 102 7 6 243 12 245 3.48e-81 244
MS.gene031492.t1 AT5G39260 53.846 221 94 2 25 244 45 258 1.45e-78 238
MS.gene031492.t1 AT4G38210 50.661 227 108 2 25 247 28 254 7.97e-78 236
MS.gene031492.t1 AT3G15370 46.886 273 101 8 6 243 12 275 1.33e-74 228
MS.gene031492.t1 AT4G28250 33.190 232 130 9 25 243 36 255 1.82e-27 106
MS.gene031492.t1 AT2G20750 33.047 233 132 9 25 243 40 262 1.20e-25 102
MS.gene031492.t1 AT4G28250 32.468 231 127 9 25 243 36 249 4.41e-24 97.4
MS.gene031492.t1 AT1G65680 30.472 233 135 10 25 242 47 267 3.14e-22 92.8
MS.gene031492.t1 AT1G65681 29.493 217 128 9 39 242 12 216 1.69e-18 81.6
MS.gene031492.t1 AT2G45110 28.333 240 133 9 24 242 32 253 2.48e-18 82.0
MS.gene031492.t1 AT4G17030 26.820 261 162 9 2 248 4 249 5.96e-18 80.9
MS.gene031492.t1 AT4G28250 33.143 175 90 7 25 189 36 193 9.19e-17 76.6
MS.gene031492.t1 AT2G20750 31.214 173 99 6 25 186 40 203 1.97e-16 75.9
MS.gene031492.t1 AT4G28250 33.140 172 88 7 25 186 36 190 3.88e-16 75.1
MS.gene031492.t1 AT3G60570 25.000 232 146 8 25 242 34 251 4.06e-16 75.9
MS.gene031492.t1 AT3G45970 26.147 218 138 10 43 247 14 221 8.50e-14 69.3
MS.gene031492.t1 AT3G45960 29.577 213 126 10 48 247 46 247 1.40e-13 68.9
MS.gene031492.t1 AT4G38400 27.830 212 131 10 48 247 47 248 1.78e-12 65.9
MS.gene031492.t1 AT3G60570 23.832 214 135 8 43 242 4 203 5.76e-12 63.5
MS.gene031492.t1 AT3G45970 25.352 213 136 10 48 247 46 248 3.30e-11 62.0

Find 66 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGCACATGCCACCTTCTA+TGG 0.202737 4.2:+27753680 MS.gene031492:CDS
GCTCCAGCTGGATGGTCTTT+TGG 0.250334 4.2:+27754542 MS.gene031492:CDS
CACCAACCACCTTGATTGTT+TGG 0.269349 4.2:-27754070 None:intergenic
GACCATAGATGGTGCCTTCC+TGG 0.298042 4.2:+27753998 MS.gene031492:CDS
GGTATAAGGTTCACTATTAA+TGG 0.300170 4.2:+27754311 MS.gene031492:CDS
GTAGGTGGGGCTTGTGGATA+TGG 0.300951 4.2:+27753869 MS.gene031492:intron
GGATATCTATGTCAAGAAAC+TGG 0.302501 4.2:+27754423 MS.gene031492:CDS
TTCCATACCCTTGACTATAA+AGG 0.310459 4.2:-27753895 None:intergenic
GGCTTACATTGGCTTGCTTT+TGG 0.310735 4.2:+27753604 MS.gene031492:CDS
GTGGCTCTTGCTATGAAATT+AGG 0.339101 4.2:+27753966 MS.gene031492:CDS
TTCTCCTCTGTTTATGCCTA+TGG 0.366961 4.2:+27753644 MS.gene031492:CDS
ATCACAAACGTTGGTGGTGC+TGG 0.390421 4.2:+27754359 MS.gene031492:CDS
AACCAGGAAGGCACCATCTA+TGG 0.400538 4.2:-27754000 None:intergenic
TCCAATCATGTTGCTCCAGC+TGG 0.405890 4.2:+27754530 MS.gene031492:CDS
CAAGAAACTGGGGACAAAAC+TGG 0.410611 4.2:+27754435 MS.gene031492:CDS
TCCAGCTGGAGCAACATGAT+TGG 0.432138 4.2:-27754531 None:intergenic
GTAGGAGAAAGGGAGGTATA+AGG 0.433696 4.2:+27754297 MS.gene031492:CDS
TAGGGTGTCATGTAGGAGAA+AGG 0.438680 4.2:+27754286 MS.gene031492:intron
TATGGAGGAAGTGATGCTTC+AGG 0.438776 4.2:+27753698 MS.gene031492:CDS
AGCATCACTTCCTCCATAGA+AGG 0.449496 4.2:-27753693 None:intergenic
TTGTTTGGTAAGGCATTGTT+TGG 0.454457 4.2:-27754055 None:intergenic
CAAAGTAACAATTATCTCAA+TGG 0.478172 4.2:+27754458 MS.gene031492:CDS
AGGGTGTCATGTAGGAGAAA+GGG 0.494086 4.2:+27754287 MS.gene031492:intron
AACCTTTATAGTCAAGGGTA+TGG 0.513145 4.2:+27753893 MS.gene031492:CDS
TCTGTTTATGCCTATGGTGG+AGG 0.513461 4.2:+27753650 MS.gene031492:CDS
CGTATTGCTCAATACAAAGC+TGG 0.513556 4.2:+27754139 MS.gene031492:CDS
AGTGATGCTTCAGGAACAAT+GGG 0.514794 4.2:+27753707 MS.gene031492:CDS
TCCTCTGTTTATGCCTATGG+TGG 0.517540 4.2:+27753647 MS.gene031492:CDS
ACCACCTTGATTGTTTGGTA+AGG 0.522218 4.2:-27754065 None:intergenic
ATCATGTTGCTCCAGCTGGA+TGG 0.525196 4.2:+27754534 MS.gene031492:CDS
GTTTGGTGGACAGAAATTAG+TGG 0.526223 4.2:-27754038 None:intergenic
GTTGAGCAAGATCAAAATGT+TGG 0.527634 4.2:-27754105 None:intergenic
TTTAAGGTTACAACTAGTGA+TGG 0.529102 4.2:+27754494 MS.gene031492:CDS
ACCTACCTTCTAAAATCTAC+AGG 0.533353 4.2:-27754169 None:intergenic
AAACAGGAAATGGCTTACAT+TGG 0.533906 4.2:+27753593 None:intergenic
TACATGCTGCATCCATCAAA+GGG 0.543754 4.2:+27754387 MS.gene031492:CDS
TATGGTAACCTTTATAGTCA+AGG 0.546440 4.2:+27753887 MS.gene031492:CDS
TTCAACAATGGCTTAAGCTG+TGG 0.546607 4.2:+27753947 MS.gene031492:CDS
GATCAAAATGTTGGAGAGGA+GGG 0.550651 4.2:-27754096 None:intergenic
GTTCTCATCACAAACGTTGG+TGG 0.552684 4.2:+27754353 MS.gene031492:CDS
TTTGGTAAGGCATTGTTTGG+TGG 0.554613 4.2:-27754052 None:intergenic
AAGTGATGCTTCAGGAACAA+TGG 0.556486 4.2:+27753706 MS.gene031492:CDS
GTACATGCTGCATCCATCAA+AGG 0.567778 4.2:+27754386 MS.gene031492:CDS
TGACCAAAAGACCATCCAGC+TGG 0.568697 4.2:-27754545 None:intergenic
GATATCTATGTCAAGAAACT+GGG 0.570919 4.2:+27754424 MS.gene031492:CDS
TTTATGCCTATGGTGGAGGA+TGG 0.581237 4.2:+27753654 MS.gene031492:CDS
AGATCAAAATGTTGGAGAGG+AGG 0.586254 4.2:-27754097 None:intergenic
ATGGTAACCTTTATAGTCAA+GGG 0.589746 4.2:+27753888 MS.gene031492:CDS
GTGCCTTCCTGGTTCCATTG+TGG 0.591922 4.2:+27754009 MS.gene031492:CDS
TACCAAACAATCAAGGTGGT+TGG 0.598522 4.2:+27754068 MS.gene031492:CDS
ATTAGTCCATCCTCCACCAT+AGG 0.598598 4.2:-27753660 None:intergenic
GCAGTGACCACAATGGAACC+AGG 0.602351 4.2:-27754016 None:intergenic
GTGTCATGTAGGAGAAAGGG+AGG 0.610710 4.2:+27754290 MS.gene031492:CDS
AATGCCTTACCAAACAATCA+AGG 0.612141 4.2:+27754061 MS.gene031492:CDS
GAGGATTTGAGCACCAGTGT+AGG 0.625146 4.2:-27754570 None:intergenic
GGTGTGCTAATGACCATAGA+TGG 0.625411 4.2:+27753987 MS.gene031492:CDS
GCAAGATCAAAATGTTGGAG+AGG 0.641783 4.2:-27754100 None:intergenic
ATCACTTCCTCCATAGAAGG+TGG 0.643748 4.2:-27753690 None:intergenic
GCACATGCCACCTTCTATGG+AGG 0.644186 4.2:+27753683 MS.gene031492:CDS
GAAAAGTGCTGTACTCAAAG+CGG 0.652141 4.2:-27753927 None:intergenic
TTAGTTCTCATCACAAACGT+TGG 0.662861 4.2:+27754350 MS.gene031492:CDS
TGACCACAATGGAACCAGGA+AGG 0.676921 4.2:-27754012 None:intergenic
ATTAGTGGCAGTGACCACAA+TGG 0.700766 4.2:-27754023 None:intergenic
ATATCTATGTCAAGAAACTG+GGG 0.706710 4.2:+27754425 MS.gene031492:CDS
GCCTTACCAAACAATCAAGG+TGG 0.713350 4.2:+27754064 MS.gene031492:CDS
TCCACCATAGGCATAAACAG+AGG 0.739179 4.2:-27753648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCAATTTTTTTGCATT+TGG + chr4.2:27754225-27754244 MS.gene031492:intron 15.0%
!! ATATAAAAAATGTAGATGCT+TGG - chr4.2:27754203-27754222 None:intergenic 20.0%
!!! AATTTGTGTTTTTATTGTGT+TGG + chr4.2:27754256-27754275 MS.gene031492:intron 20.0%
!!! TAGTGGTTTTTATTGAAAAA+TGG - chr4.2:27753739-27753758 None:intergenic 20.0%
! CAAAGTAACAATTATCTCAA+TGG + chr4.2:27754458-27754477 MS.gene031492:CDS 25.0%
!! CAAAACTTTATTTCAAGTAG+TGG - chr4.2:27753756-27753775 None:intergenic 25.0%
AAACAACAAAGTCAGTGAAA+TGG - chr4.2:27753844-27753863 None:intergenic 30.0%
AACAACAAAGTCAGTGAAAT+GGG - chr4.2:27753843-27753862 None:intergenic 30.0%
ATATCTATGTCAAGAAACTG+GGG + chr4.2:27754425-27754444 MS.gene031492:CDS 30.0%
ATGGTAACCTTTATAGTCAA+GGG + chr4.2:27753888-27753907 MS.gene031492:CDS 30.0%
GATATCTATGTCAAGAAACT+GGG + chr4.2:27754424-27754443 MS.gene031492:CDS 30.0%
GGTATAAGGTTCACTATTAA+TGG + chr4.2:27754311-27754330 MS.gene031492:CDS 30.0%
TATGGTAACCTTTATAGTCA+AGG + chr4.2:27753887-27753906 MS.gene031492:CDS 30.0%
! TGGTCAAAGTTTGTCTTTTA+AGG + chr4.2:27754478-27754497 MS.gene031492:CDS 30.0%
! TTGTTCCTGTAGATTTTAGA+AGG + chr4.2:27754164-27754183 MS.gene031492:CDS 30.0%
! TTTAAGGTTACAACTAGTGA+TGG + chr4.2:27754494-27754513 MS.gene031492:CDS 30.0%
!!! GACTTTGTTGTTTTTTGTGT+AGG + chr4.2:27753851-27753870 MS.gene031492:intron 30.0%
!!! TATTGTGTTGGTTTTGTGTA+GGG + chr4.2:27754268-27754287 MS.gene031492:intron 30.0%
!!! TTATTGTGTTGGTTTTGTGT+AGG + chr4.2:27754267-27754286 MS.gene031492:intron 30.0%
!!! TTGTTGTTTTTTGTGTAGGT+GGG + chr4.2:27753855-27753874 MS.gene031492:intron 30.0%
!!! TTTGTTGTTTTTTGTGTAGG+TGG + chr4.2:27753854-27753873 MS.gene031492:intron 30.0%
AACCTTTATAGTCAAGGGTA+TGG + chr4.2:27753893-27753912 MS.gene031492:CDS 35.0%
AATGCCTTACCAAACAATCA+AGG + chr4.2:27754061-27754080 MS.gene031492:CDS 35.0%
GGATATCTATGTCAAGAAAC+TGG + chr4.2:27754423-27754442 MS.gene031492:CDS 35.0%
GTTGAGCAAGATCAAAATGT+TGG - chr4.2:27754108-27754127 None:intergenic 35.0%
TGAGCAATACGAAGAAAAAC+AGG - chr4.2:27754130-27754149 None:intergenic 35.0%
TTCCATACCCTTGACTATAA+AGG - chr4.2:27753898-27753917 None:intergenic 35.0%
! AGTACAGCACTTTTCAACAA+TGG + chr4.2:27753935-27753954 MS.gene031492:CDS 35.0%
! TTAGTTCTCATCACAAACGT+TGG + chr4.2:27754350-27754369 MS.gene031492:CDS 35.0%
!! ACCTACCTTCTAAAATCTAC+AGG - chr4.2:27754172-27754191 None:intergenic 35.0%
!! TCCTGTAGATTTTAGAAGGT+AGG + chr4.2:27754168-27754187 MS.gene031492:intron 35.0%
!! TTGTTTGGTAAGGCATTGTT+TGG - chr4.2:27754058-27754077 None:intergenic 35.0%
!!! TGTTGTTTTTTGTGTAGGTG+GGG + chr4.2:27753856-27753875 MS.gene031492:intron 35.0%
AAGTGATGCTTCAGGAACAA+TGG + chr4.2:27753706-27753725 MS.gene031492:CDS 40.0%
ACCACCTTGATTGTTTGGTA+AGG - chr4.2:27754068-27754087 None:intergenic 40.0%
AGATCAAAATGTTGGAGAGG+AGG - chr4.2:27754100-27754119 None:intergenic 40.0%
AGTGATGCTTCAGGAACAAT+GGG + chr4.2:27753707-27753726 MS.gene031492:CDS 40.0%
CGTATTGCTCAATACAAAGC+TGG + chr4.2:27754139-27754158 MS.gene031492:CDS 40.0%
GATCAAAATGTTGGAGAGGA+GGG - chr4.2:27754099-27754118 None:intergenic 40.0%
GCAAGATCAAAATGTTGGAG+AGG - chr4.2:27754103-27754122 None:intergenic 40.0%
TACATGCTGCATCCATCAAA+GGG + chr4.2:27754387-27754406 MS.gene031492:CDS 40.0%
TACCAAACAATCAAGGTGGT+TGG + chr4.2:27754068-27754087 MS.gene031492:CDS 40.0%
TCAAAGGGTCAAAAACTGGT+TGG + chr4.2:27754402-27754421 MS.gene031492:CDS 40.0%
TCCATCAAAGGGTCAAAAAC+TGG + chr4.2:27754398-27754417 MS.gene031492:CDS 40.0%
TTCAACAATGGCTTAAGCTG+TGG + chr4.2:27753947-27753966 MS.gene031492:CDS 40.0%
TTCTCCTCTGTTTATGCCTA+TGG + chr4.2:27753644-27753663 MS.gene031492:CDS 40.0%
! ACCAGTTTTTGACCCTTTGA+TGG - chr4.2:27754402-27754421 None:intergenic 40.0%
! GTGGCTCTTGCTATGAAATT+AGG + chr4.2:27753966-27753985 MS.gene031492:CDS 40.0%
! GTTTGGTGGACAGAAATTAG+TGG - chr4.2:27754041-27754060 None:intergenic 40.0%
! TCTTTTGGTCAAACCTACAC+TGG + chr4.2:27754557-27754576 MS.gene031492:CDS 40.0%
!! GAAAAGTGCTGTACTCAAAG+CGG - chr4.2:27753930-27753949 None:intergenic 40.0%
!! TTTGGTAAGGCATTGTTTGG+TGG - chr4.2:27754055-27754074 None:intergenic 40.0%
!! TTTTGTGTAGGGTGTCATGT+AGG + chr4.2:27754279-27754298 MS.gene031492:intron 40.0%
AATGCACATGCCACCTTCTA+TGG + chr4.2:27753680-27753699 MS.gene031492:CDS 45.0%
AGCATCACTTCCTCCATAGA+AGG - chr4.2:27753696-27753715 None:intergenic 45.0%
ATCACTTCCTCCATAGAAGG+TGG - chr4.2:27753693-27753712 None:intergenic 45.0%
ATTAGTCCATCCTCCACCAT+AGG - chr4.2:27753663-27753682 None:intergenic 45.0%
ATTAGTGGCAGTGACCACAA+TGG - chr4.2:27754026-27754045 None:intergenic 45.0%
CAAGAAACTGGGGACAAAAC+TGG + chr4.2:27754435-27754454 MS.gene031492:CDS 45.0%
CACCAACCACCTTGATTGTT+TGG - chr4.2:27754073-27754092 None:intergenic 45.0%
GCCTTACCAAACAATCAAGG+TGG + chr4.2:27754064-27754083 MS.gene031492:CDS 45.0%
GGTGTGCTAATGACCATAGA+TGG + chr4.2:27753987-27754006 MS.gene031492:CDS 45.0%
GTACATGCTGCATCCATCAA+AGG + chr4.2:27754386-27754405 MS.gene031492:CDS 45.0%
GTTCTCATCACAAACGTTGG+TGG + chr4.2:27754353-27754372 MS.gene031492:CDS 45.0%
TATGGAGGAAGTGATGCTTC+AGG + chr4.2:27753698-27753717 MS.gene031492:CDS 45.0%
TCCACCATAGGCATAAACAG+AGG - chr4.2:27753651-27753670 None:intergenic 45.0%
TCCTCTGTTTATGCCTATGG+TGG + chr4.2:27753647-27753666 MS.gene031492:CDS 45.0%
TCTGTTTATGCCTATGGTGG+AGG + chr4.2:27753650-27753669 MS.gene031492:CDS 45.0%
! AGGGTGTCATGTAGGAGAAA+GGG + chr4.2:27754287-27754306 MS.gene031492:intron 45.0%
! GTAGGAGAAAGGGAGGTATA+AGG + chr4.2:27754297-27754316 MS.gene031492:CDS 45.0%
! TAGGGTGTCATGTAGGAGAA+AGG + chr4.2:27754286-27754305 MS.gene031492:intron 45.0%
! TTTATGCCTATGGTGGAGGA+TGG + chr4.2:27753654-27753673 MS.gene031492:CDS 45.0%
!!! GGCTTACATTGGCTTGCTTT+TGG + chr4.2:27753604-27753623 MS.gene031492:CDS 45.0%
ATCATGTTGCTCCAGCTGGA+TGG + chr4.2:27754534-27754553 MS.gene031492:CDS 50.0%
GAGGATTTGAGCACCAGTGT+AGG - chr4.2:27754573-27754592 None:intergenic 50.0%
GTGTCATGTAGGAGAAAGGG+AGG + chr4.2:27754290-27754309 MS.gene031492:CDS 50.0%
TCCAATCATGTTGCTCCAGC+TGG + chr4.2:27754530-27754549 MS.gene031492:CDS 50.0%
TCCAGCTGGAGCAACATGAT+TGG - chr4.2:27754534-27754553 None:intergenic 50.0%
TGACCAAAAGACCATCCAGC+TGG - chr4.2:27754548-27754567 None:intergenic 50.0%
TGACCACAATGGAACCAGGA+AGG - chr4.2:27754015-27754034 None:intergenic 50.0%
! TTTTGTGTAGGTGGGGCTTG+TGG + chr4.2:27753863-27753882 MS.gene031492:intron 50.0%
!! AACCAGGAAGGCACCATCTA+TGG - chr4.2:27754003-27754022 None:intergenic 50.0%
!! ATCACAAACGTTGGTGGTGC+TGG + chr4.2:27754359-27754378 MS.gene031492:CDS 50.0%
GACCATAGATGGTGCCTTCC+TGG + chr4.2:27753998-27754017 MS.gene031492:CDS 55.0%
GCACATGCCACCTTCTATGG+AGG + chr4.2:27753683-27753702 MS.gene031492:CDS 55.0%
GCAGTGACCACAATGGAACC+AGG - chr4.2:27754019-27754038 None:intergenic 55.0%
GTAGGTGGGGCTTGTGGATA+TGG + chr4.2:27753869-27753888 MS.gene031492:intron 55.0%
GTGCCTTCCTGGTTCCATTG+TGG + chr4.2:27754009-27754028 MS.gene031492:CDS 55.0%
! GCTCCAGCTGGATGGTCTTT+TGG + chr4.2:27754542-27754561 MS.gene031492:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27753602 27754595 27753602 ID=MS.gene031492
chr4.2 mRNA 27753602 27754595 27753602 ID=MS.gene031492.t1;Parent=MS.gene031492
chr4.2 exon 27753602 27753728 27753602 ID=MS.gene031492.t1.exon1;Parent=MS.gene031492.t1
chr4.2 CDS 27753602 27753728 27753602 ID=cds.MS.gene031492.t1;Parent=MS.gene031492.t1
chr4.2 exon 27753873 27754185 27753873 ID=MS.gene031492.t1.exon2;Parent=MS.gene031492.t1
chr4.2 CDS 27753873 27754185 27753873 ID=cds.MS.gene031492.t1;Parent=MS.gene031492.t1
chr4.2 exon 27754289 27754595 27754289 ID=MS.gene031492.t1.exon3;Parent=MS.gene031492.t1
chr4.2 CDS 27754289 27754595 27754289 ID=cds.MS.gene031492.t1;Parent=MS.gene031492.t1
Gene Sequence

>MS.gene031492

ATGGCTTACATTGGCTTGCTTTTGGTGTGTTCTCTCACTATGTTCTCCTCTGTTTATGCCTATGGTGGAGGATGGACTAATGCACATGCCACCTTCTATGGAGGAAGTGATGCTTCAGGAACAATGGGTATGCACCATTTTTCAATAAAAACCACTACTTGAAATAAAGTTTTGATTTTTAAGCTTTTTTGTAAAAAAAAAAAAAAAAAAAACAGATCTTGAAGCAAAAAACAAGTAACCCATTTCACTGACTTTGTTGTTTTTTGTGTAGGTGGGGCTTGTGGATATGGTAACCTTTATAGTCAAGGGTATGGAACAAACACTGCCGCTTTGAGTACAGCACTTTTCAACAATGGCTTAAGCTGTGGCTCTTGCTATGAAATTAGGTGTGCTAATGACCATAGATGGTGCCTTCCTGGTTCCATTGTGGTCACTGCCACTAATTTCTGTCCACCAAACAATGCCTTACCAAACAATCAAGGTGGTTGGTGTAACCCTCCTCTCCAACATTTTGATCTTGCTCAACCTGTTTTTCTTCGTATTGCTCAATACAAAGCTGGAATTGTTCCTGTAGATTTTAGAAGGTAGGTTGCTTTTACCAAGCATCTACATTTTTTATATAAATTTTCAATTTTTTTGCATTTGGTATAAACTAATTTGTGTTTTTATTGTGTTGGTTTTGTGTAGGGTGTCATGTAGGAGAAAGGGAGGTATAAGGTTCACTATTAATGGACATTCTTACTTCAACTTAGTTCTCATCACAAACGTTGGTGGTGCTGGAGATGTACATGCTGCATCCATCAAAGGGTCAAAAACTGGTTGGATATCTATGTCAAGAAACTGGGGACAAAACTGGCAAAGTAACAATTATCTCAATGGTCAAAGTTTGTCTTTTAAGGTTACAACTAGTGATGGTCGTACTGTTGTTTCCAATCATGTTGCTCCAGCTGGATGGTCTTTTGGTCAAACCTACACTGGTGCTCAAATCCTCTAG
Protein sequence

>MS.gene031492.t1

MAYIGLLLVCSLTMFSSVYAYGGGWTNAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGSCYEIRCANDHRWCLPGSIVVTATNFCPPNNALPNNQGGWCNPPLQHFDLAQPVFLRIAQYKAGIVPVDFRRVSCRRKGGIRFTINGHSYFNLVLITNVGGAGDVHAASIKGSKTGWISMSRNWGQNWQSNNYLNGQSLSFKVTTSDGRTVVSNHVAPAGWSFGQTYTGAQIL