AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032304

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032304.t1 MTR_7g111010 52.778 252 111 3 11 255 4 254 4.68e-98 287
MS.gene032304.t1 MTR_1g085460 54.357 241 104 2 20 255 18 257 1.46e-97 286
MS.gene032304.t1 MTR_7g110990 52.675 243 103 3 18 255 4 239 1.07e-94 278
MS.gene032304.t1 MTR_1g026020 52.632 247 107 4 17 255 11 255 7.81e-94 276
MS.gene032304.t1 MTR_3g435430 53.846 234 98 5 22 250 15 243 1.85e-88 263
MS.gene032304.t1 MTR_5g041700 56.444 225 88 5 31 250 24 243 6.02e-87 259
MS.gene032304.t1 MTR_4g081950 50.787 254 114 6 3 250 31 279 8.13e-87 259
MS.gene032304.t1 MTR_3g094040 50.000 254 115 6 4 250 1 249 1.59e-85 255
MS.gene032304.t1 MTR_3g094060 50.787 254 113 7 4 250 1 249 2.43e-85 255
MS.gene032304.t1 MTR_7g011060 53.556 239 99 6 16 250 6 236 8.07e-85 253
MS.gene032304.t1 MTR_5g075320 51.440 243 105 7 15 249 9 246 1.12e-84 253
MS.gene032304.t1 MTR_3g466790 50.000 250 110 6 10 249 5 249 6.33e-83 249
MS.gene032304.t1 MTR_5g079950 47.843 255 123 5 5 253 6 256 1.17e-81 246
MS.gene032304.t1 MTR_3g466760 49.796 245 108 6 15 249 16 255 3.13e-81 244
MS.gene032304.t1 MTR_7g061720 50.877 228 102 5 32 253 32 255 1.59e-80 243
MS.gene032304.t1 MTR_2g097030 47.967 246 116 6 16 253 16 257 2.25e-78 237
MS.gene032304.t1 MTR_7g013150 46.988 249 121 6 11 255 12 253 2.28e-77 234
MS.gene032304.t1 MTR_6g012520 55.310 226 93 5 31 256 27 244 8.71e-76 230
MS.gene032304.t1 MTR_4g063975 50.439 228 103 5 32 253 32 255 4.04e-75 229
MS.gene032304.t1 MTR_7g013140 45.714 245 124 6 15 255 12 251 2.79e-74 226
MS.gene032304.t1 MTR_7g013120 46.122 245 124 5 15 255 13 253 3.25e-74 226
MS.gene032304.t1 MTR_8g058990 47.951 244 118 6 16 255 15 253 1.30e-72 223
MS.gene032304.t1 MTR_8g464350 46.850 254 120 6 13 255 5 254 4.25e-72 221
MS.gene032304.t1 MTR_4g124340 45.259 232 114 6 29 253 58 283 1.07e-70 219
MS.gene032304.t1 MTR_4g102450 45.914 257 123 7 11 256 11 262 3.83e-70 216
MS.gene032304.t1 MTR_5g011210 43.077 260 135 7 4 256 10 263 5.25e-69 214
MS.gene032304.t1 MTR_0040s0140 44.706 255 126 7 1 249 6 251 1.10e-68 213
MS.gene032304.t1 MTR_2g090255 43.802 242 126 5 15 250 11 248 5.29e-67 208
MS.gene032304.t1 MTR_3g064510 46.983 232 106 7 32 250 53 280 6.82e-67 209
MS.gene032304.t1 MTR_5g076950 46.552 232 111 5 32 256 41 266 1.88e-66 207
MS.gene032304.t1 MTR_0008s0120 34.694 245 103 4 11 255 12 199 4.06e-39 135
MS.gene032304.t1 MTR_2g090270 31.801 261 147 10 15 255 9 258 6.70e-31 115
MS.gene032304.t1 MTR_0122s0020 35.745 235 131 8 32 256 41 265 5.42e-30 113
MS.gene032304.t1 MTR_2g090265 32.046 259 147 10 15 255 9 256 2.03e-28 108
MS.gene032304.t1 MTR_6g012500 48.544 103 52 1 154 256 181 282 1.03e-27 107
MS.gene032304.t1 MTR_4g028640 33.077 260 147 11 9 255 19 264 1.51e-25 101
MS.gene032304.t1 MTR_0809s0010 29.804 255 153 10 14 255 29 270 4.03e-21 89.7
MS.gene032304.t1 MTR_0118s0070 30.702 228 136 8 37 251 53 271 2.73e-20 87.8
MS.gene032304.t1 MTR_5g013440 29.134 254 148 12 15 255 17 251 3.27e-18 81.6
MS.gene032304.t1 MTR_0122s0020 36.207 174 91 8 32 195 41 204 1.27e-17 79.3
MS.gene032304.t1 MTR_1g085365 53.704 54 25 0 199 252 4 57 1.18e-12 61.6
MS.gene032304.t1 MTR_4g099370 24.621 264 166 11 8 255 4 250 1.30e-12 66.2
MS.gene032304.t1 MTR_4g075790 28.936 235 138 10 34 255 25 243 9.28e-12 63.9
MS.gene032304.t1 MTR_4g098930 26.718 262 155 10 15 254 5 251 1.63e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032304.t1 AT3G15370 66.805 241 80 0 16 256 11 251 1.03e-122 350
MS.gene032304.t1 AT3G15370 59.851 269 78 1 16 254 11 279 5.69e-116 333
MS.gene032304.t1 AT4G01630 50.397 252 119 2 9 255 5 255 2.70e-96 283
MS.gene032304.t1 AT1G20190 54.812 239 98 4 25 255 16 252 2.61e-89 265
MS.gene032304.t1 AT1G69530 54.098 244 99 6 15 250 7 245 3.62e-88 263
MS.gene032304.t1 AT1G69530 54.098 244 99 6 15 250 7 245 3.82e-88 262
MS.gene032304.t1 AT1G69530 54.098 244 99 6 15 250 7 245 3.82e-88 262
MS.gene032304.t1 AT2G40610 51.464 239 107 4 16 249 14 248 2.05e-87 260
MS.gene032304.t1 AT5G56320 51.250 240 107 5 16 250 13 247 1.82e-86 258
MS.gene032304.t1 AT5G56320 51.250 240 107 5 16 250 13 247 1.82e-86 258
MS.gene032304.t1 AT5G56320 51.250 240 107 5 16 250 13 247 7.35e-86 258
MS.gene032304.t1 AT2G03090 51.765 255 106 7 1 250 6 248 5.65e-85 254
MS.gene032304.t1 AT2G39700 51.417 247 108 6 15 253 12 254 1.95e-83 250
MS.gene032304.t1 AT3G55500 48.263 259 124 5 1 253 3 257 3.29e-83 249
MS.gene032304.t1 AT3G29030 47.909 263 110 6 1 250 1 249 2.22e-82 247
MS.gene032304.t1 AT5G05290 49.587 242 112 5 15 250 14 251 2.82e-82 247
MS.gene032304.t1 AT5G02260 50.877 228 102 5 32 253 32 255 3.82e-81 244
MS.gene032304.t1 AT2G37640 51.316 228 101 5 32 253 36 259 2.79e-79 240
MS.gene032304.t1 AT1G26770 50.207 241 110 5 15 250 9 244 2.59e-78 237
MS.gene032304.t1 AT1G26770 50.207 241 110 5 15 250 19 254 7.02e-78 236
MS.gene032304.t1 AT2G28950 48.163 245 115 6 17 253 14 254 3.66e-76 232
MS.gene032304.t1 AT5G39280 49.339 227 107 5 32 255 54 275 2.26e-75 230
MS.gene032304.t1 AT5G39300 48.458 227 109 5 32 255 55 276 2.54e-75 230
MS.gene032304.t1 AT1G20190 56.701 194 77 3 67 255 2 193 2.29e-74 225
MS.gene032304.t1 AT1G69530 54.854 206 80 6 15 212 7 207 5.87e-73 223
MS.gene032304.t1 AT1G69530 54.854 206 80 6 15 212 7 207 6.22e-73 222
MS.gene032304.t1 AT5G39290 48.899 227 108 5 32 255 58 279 6.34e-72 221
MS.gene032304.t1 AT2G37640 51.659 211 92 5 49 253 28 234 7.38e-72 220
MS.gene032304.t1 AT5G39270 48.899 227 108 5 32 255 58 279 9.90e-72 221
MS.gene032304.t1 AT1G12560 43.233 266 135 7 1 255 1 261 4.27e-71 219
MS.gene032304.t1 AT3G03220 47.345 226 106 5 32 250 40 259 3.86e-67 209
MS.gene032304.t1 AT1G62980 42.063 252 132 6 14 255 9 256 2.11e-65 204
MS.gene032304.t1 AT5G39310 42.489 233 125 6 27 255 85 312 5.32e-63 200
MS.gene032304.t1 AT4G38210 44.177 249 126 7 16 256 12 255 2.01e-62 196
MS.gene032304.t1 AT5G39260 44.444 225 117 6 32 255 45 262 4.00e-61 193
MS.gene032304.t1 AT4G28250 36.667 270 140 11 3 255 5 260 2.50e-37 132
MS.gene032304.t1 AT2G20750 37.344 241 133 9 26 255 34 267 1.45e-34 125
MS.gene032304.t1 AT4G28250 34.944 269 140 11 3 255 5 254 2.07e-32 119
MS.gene032304.t1 AT1G65680 35.294 238 129 11 32 255 47 273 3.74e-26 103
MS.gene032304.t1 AT2G20750 38.983 177 91 8 26 192 34 203 6.24e-24 96.3
MS.gene032304.t1 AT4G28250 33.971 209 104 10 3 196 5 194 1.14e-21 90.1
MS.gene032304.t1 AT4G28250 34.634 205 100 10 3 192 5 190 2.51e-21 89.0
MS.gene032304.t1 AT1G65681 29.680 219 133 10 48 255 14 222 1.07e-18 82.4
MS.gene032304.t1 AT3G60570 25.941 239 147 8 32 255 34 257 5.90e-17 78.6
MS.gene032304.t1 AT4G17030 26.744 258 151 11 11 252 11 246 1.62e-13 68.6
MS.gene032304.t1 AT3G60570 25.339 221 135 8 50 255 4 209 3.58e-13 67.0
MS.gene032304.t1 AT3G45960 28.500 200 130 7 63 255 6 199 3.61e-12 64.3
MS.gene032304.t1 AT3G45960 28.500 200 130 7 63 255 54 247 4.88e-12 64.7
MS.gene032304.t1 AT4G38400 26.000 200 135 7 63 255 55 248 3.01e-11 62.4

Find 63 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTACCATTGCACAGAAAT+TGG 0.160155 8.2:+47830620 MS.gene032304:CDS
AACGCTCATGCAACGTTCTA+TGG 0.182142 8.2:+47827725 MS.gene032304:CDS
CGACTTCTAAGACACCATTT+TGG 0.231012 8.2:-47828919 None:intergenic
GAAACTAATAGAATTGATTA+TGG 0.275627 8.2:-47830777 None:intergenic
TAATCTTGAACGAGAGTCTT+TGG 0.293184 8.2:-47830678 None:intergenic
TGGTGGCAGCGGTGATATAA+AGG 0.294731 8.2:+47830564 MS.gene032304:CDS
ACTATAAAATCGACCCAAAA+TGG 0.309177 8.2:+47828905 MS.gene032304:CDS
TTCAAGATTACTTTGGTTGA+TGG 0.313364 8.2:+47830691 MS.gene032304:CDS
ACAGAAATTGGGGAGCTAAT+TGG 0.336521 8.2:+47830632 MS.gene032304:CDS
CACCATCCTCCATGATTGTT+TGG 0.340496 8.2:-47828976 None:intergenic
GTTACCATTGCACAGAAATT+GGG 0.345806 8.2:+47830621 MS.gene032304:CDS
AAGGCTGGTTGGACTTTGAT+TGG 0.372747 8.2:-47827750 None:intergenic
TGGTAACCAAGCTCCACTTC+TGG 0.376497 8.2:-47830605 None:intergenic
GGCAAAGCAGTGCAGATCTA+AGG 0.383218 8.2:+47830653 MS.gene032304:CDS
GTGTCTTCCAATTGGAGATT+TGG 0.391994 8.2:+47830739 MS.gene032304:CDS
GATACCTTTCATGCTGGGTT+TGG 0.393917 8.2:+47828805 MS.gene032304:CDS
TTGTTCCTGTCCTTTATAGA+AGG 0.394122 8.2:+47829073 MS.gene032304:CDS
GCAAAAGAAGAGGAGGAGTT+AGG 0.422267 8.2:+47830491 MS.gene032304:CDS
TCAATGTTGTGTCTTCCAAT+TGG 0.423263 8.2:+47830731 MS.gene032304:CDS
GGTGGCAGCGGTGATATAAA+GGG 0.440322 8.2:+47830565 MS.gene032304:CDS
TATGATGATACCTTTCATGC+TGG 0.440717 8.2:+47828799 MS.gene032304:CDS
TTCAGAGATGGTGAGGTTTG+TGG 0.448953 8.2:+47828859 MS.gene032304:CDS
AGGAGTTAGGTTCACATTGA+AGG 0.456036 8.2:+47830504 MS.gene032304:CDS
ACGCTCATGCAACGTTCTAT+GGG 0.460595 8.2:+47827726 MS.gene032304:CDS
ATGATGATACCTTTCATGCT+GGG 0.461474 8.2:+47828800 MS.gene032304:CDS
AAACTAATAGAATTGATTAT+GGG 0.477188 8.2:-47830776 None:intergenic
ACTCTCGTTCAAGATTACTT+TGG 0.482452 8.2:+47830684 MS.gene032304:CDS
TTCTACCTTCTATAAAGGAC+AGG 0.483939 8.2:-47829078 None:intergenic
TGTTTGTCCAAATCTCCAAT+TGG 0.490797 8.2:-47830746 None:intergenic
GTCATAACTCATACCAAGGC+TGG 0.504359 8.2:-47827765 None:intergenic
ATAACAAACGTTGGTGGCAG+CGG 0.525888 8.2:+47830553 MS.gene032304:CDS
GGAGTTAGGTTCACATTGAA+GGG 0.526521 8.2:+47830505 MS.gene032304:CDS
AATCAAAGTCCAACCAGCCT+TGG 0.527653 8.2:+47827752 MS.gene032304:CDS
CACACCAAACCCAGCATGAA+AGG 0.535814 8.2:-47828809 None:intergenic
TTCTTTCGCATTGCACGACA+AGG 0.541785 8.2:+47829039 MS.gene032304:CDS
CGTGCAATGCGAAAGAAAGC+AGG 0.542098 8.2:-47829033 None:intergenic
AATATTTCAGGAGGAGCTTG+TGG 0.542340 8.2:+47828775 MS.gene032304:intron
GAGGTTCCAGAAGTGGAGCT+TGG 0.549845 8.2:+47830599 MS.gene032304:CDS
GCTCCCCAATTTCTGTGCAA+TGG 0.550029 8.2:-47830625 None:intergenic
CATCCTCCATGATTGTTTGG+AGG 0.550332 8.2:-47828973 None:intergenic
TGGATAAGAGGTTCCAGAAG+TGG 0.560366 8.2:+47830592 MS.gene032304:CDS
ATTGCACGACAAGGAAATGA+AGG 0.572173 8.2:+47829048 MS.gene032304:CDS
AGAATCCACGTTGAAGCTAA+CGG 0.575394 8.2:+47827695 MS.gene032304:CDS
TAACTCATACCAAGGCTGGT+TGG 0.575835 8.2:-47827761 None:intergenic
TTACCATTGCACAGAAATTG+GGG 0.582471 8.2:+47830622 MS.gene032304:CDS
TCCACGTTGAAGCTAACGGT+TGG 0.587064 8.2:+47827699 MS.gene032304:CDS
CTCCAAACAATCATGGAGGA+TGG 0.600779 8.2:+47828974 MS.gene032304:CDS
TTTGGTTGATGGTAAAACAT+TGG 0.607982 8.2:+47830702 MS.gene032304:CDS
GCATGGACATATCAAAGTGA+TGG 0.615233 8.2:-47829011 None:intergenic
TTAAATGAAGAAATCACAAG+AGG 0.618476 8.2:-47827632 None:intergenic
AGGGTAGCATGCAAAAGAAG+AGG 0.620539 8.2:+47830481 MS.gene032304:intron
GTAGCATGCAAAAGAAGAGG+AGG 0.621264 8.2:+47830484 MS.gene032304:CDS
GACATATCAAAGTGATGGTG+TGG 0.621785 8.2:-47829006 None:intergenic
ATATCAAAGTGATGGTGTGG+AGG 0.623230 8.2:-47829003 None:intergenic
CGCTCATGCAACGTTCTATG+GGG 0.631588 8.2:+47827727 MS.gene032304:CDS
AATGCGAAAGAAAGCAGGCA+TGG 0.655376 8.2:-47829028 None:intergenic
GTGGCAGCGGTGATATAAAG+GGG 0.655664 8.2:+47830566 MS.gene032304:CDS
GGGTCAATCAAATTTCAACA+TGG 0.676126 8.2:+47830525 MS.gene032304:CDS
GTGATGATAACAAACGTTGG+TGG 0.676699 8.2:+47830547 MS.gene032304:CDS
TGTCCTCCAAACAATCATGG+AGG 0.680110 8.2:+47828970 MS.gene032304:CDS
GCCAACCGTTAGCTTCAACG+TGG 0.691827 8.2:-47827700 None:intergenic
TGGCAGCGGTGATATAAAGG+GGG 0.752235 8.2:+47830567 MS.gene032304:CDS
ATGGTGATGATAACAAACGT+TGG 0.779869 8.2:+47830544 MS.gene032304:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAGAAAAATTTGTTTA+AGG + chr8.2:47830005-47830024 MS.gene032304:intron 10.0%
!!! TATTTTTTTTGTTATATTGT+TGG - chr8.2:47829945-47829964 None:intergenic 10.0%
!!! TTATAGAAAAATTTGTTTAA+GGG + chr8.2:47830006-47830025 MS.gene032304:intron 10.0%
!!! TTCAATTTTATCTATAATTA+TGG + chr8.2:47827857-47827876 MS.gene032304:intron 10.0%
!!! TTTCTTCATTTAATTTTATT+TGG + chr8.2:47827642-47827661 MS.gene032304:CDS 10.0%
!! AAAACATATCTTTCTTTAAT+AGG + chr8.2:47829102-47829121 MS.gene032304:intron 15.0%
!! AATAATACTTCTCAATAAAA+AGG - chr8.2:47828183-47828202 None:intergenic 15.0%
!! AATAGTAATAAAATTCAACA+TGG + chr8.2:47828469-47828488 MS.gene032304:intron 15.0%
!! AGAAAAAGTTGTAAAAAAAA+TGG - chr8.2:47828213-47828232 None:intergenic 15.0%
!! AGTTAAGATTAGAATATATA+AGG - chr8.2:47828669-47828688 None:intergenic 15.0%
!! ATAAATTTACAAAGAAAAAG+AGG - chr8.2:47828293-47828312 None:intergenic 15.0%
!! ATAATACTTCTCAATAAAAA+GGG - chr8.2:47828182-47828201 None:intergenic 15.0%
!! ATTATATCCCTAAAATAAAA+AGG - chr8.2:47827990-47828009 None:intergenic 15.0%
!! CATATAGTATAGATAATTAA+CGG + chr8.2:47830268-47830287 MS.gene032304:intron 15.0%
!! GTTATTTGTATTGAAAATTA+AGG + chr8.2:47830187-47830206 MS.gene032304:intron 15.0%
!! TAAAGGAAAAAAAGATTTAT+AGG + chr8.2:47829623-47829642 MS.gene032304:intron 15.0%
!! TATAAATAAAAAATCTGATG+AGG + chr8.2:47829337-47829356 MS.gene032304:intron 15.0%
!! TGTTATATTAAATTATCAGA+AGG - chr8.2:47828732-47828751 None:intergenic 15.0%
!!! AAAAATAGTGATTTCTTTTT+CGG - chr8.2:47829317-47829336 None:intergenic 15.0%
!!! AATTGTACCTTTTTATTTTA+GGG + chr8.2:47827980-47827999 MS.gene032304:intron 15.0%
!!! CAGTATTTTTTATGAAATTT+TGG + chr8.2:47829834-47829853 MS.gene032304:intron 15.0%
!!! TAACATTTTCAAAAACAATA+TGG - chr8.2:47830347-47830366 None:intergenic 15.0%
!!! TAACATTTTTAGTTAAATAG+TGG - chr8.2:47829716-47829735 None:intergenic 15.0%
!!! TAATTGTACCTTTTTATTTT+AGG + chr8.2:47827979-47827998 MS.gene032304:intron 15.0%
!!! TACACTTTTAATATGATAAA+AGG + chr8.2:47830079-47830098 MS.gene032304:intron 15.0%
!!! TAGTTTTAAATTTATCAAGA+AGG + chr8.2:47827919-47827938 MS.gene032304:intron 15.0%
!!! TCTTTTTCTTTGTAAATTTA+TGG + chr8.2:47828292-47828311 MS.gene032304:intron 15.0%
!!! TGGTCTATATAAAATATTTT+TGG - chr8.2:47828580-47828599 None:intergenic 15.0%
!! ATATATACATGTAATTGAAC+AGG + chr8.2:47830461-47830480 MS.gene032304:intron 20.0%
!! ATGTTTATAGGTTTCATTAA+AGG + chr8.2:47829606-47829625 MS.gene032304:intron 20.0%
!! ATTTATGTAAAGATTTAGAG+AGG - chr8.2:47828603-47828622 None:intergenic 20.0%
!! CTCTTTATGTATCATATAAA+GGG - chr8.2:47829503-47829522 None:intergenic 20.0%
!! CTTTATATGATACATAAAGA+GGG + chr8.2:47829502-47829521 MS.gene032304:intron 20.0%
!! GGTTCTTAATATTAAACAAA+TGG - chr8.2:47828711-47828730 None:intergenic 20.0%
!! TAATACTTCTCAATAAAAAG+GGG - chr8.2:47828181-47828200 None:intergenic 20.0%
!! TATATACATGTAATTGAACA+GGG + chr8.2:47830462-47830481 MS.gene032304:intron 20.0%
!! TTAAATTAATCCACTCAAAT+TGG + chr8.2:47830364-47830383 MS.gene032304:intron 20.0%
!! TTCTATAATAAAGATTGTGT+TGG - chr8.2:47829994-47830013 None:intergenic 20.0%
!!! ATAATTTTTACACGCATATA+AGG - chr8.2:47828017-47828036 None:intergenic 20.0%
!!! ATTTGTTTGTCATTTGATTA+AGG - chr8.2:47829430-47829449 None:intergenic 20.0%
!!! TATTTGGTGTCTTTTATTAT+TGG + chr8.2:47827658-47827677 MS.gene032304:CDS 20.0%
!!! TTGAAAATTAAGGACAATTT+TGG + chr8.2:47830197-47830216 MS.gene032304:intron 20.0%
!!! TTTAGATATTTTTATAGAGC+TGG + chr8.2:47829214-47829233 MS.gene032304:intron 20.0%
!!! TTTTATTCTACCTTCTATAA+AGG - chr8.2:47829086-47829105 None:intergenic 20.0%
! AGGAAAAAAAGATTTATAGG+TGG + chr8.2:47829626-47829645 MS.gene032304:intron 25.0%
! AGGATGCAACATTATAATAT+GGG - chr8.2:47830418-47830437 None:intergenic 25.0%
! ATCCTCTATTGTAAATCAAT+TGG + chr8.2:47830433-47830452 MS.gene032304:intron 25.0%
! CCTCTTTATGTATCATATAA+AGG - chr8.2:47829504-47829523 None:intergenic 25.0%
! CCTTTATATGATACATAAAG+AGG + chr8.2:47829501-47829520 MS.gene032304:intron 25.0%
! CTGAAATTACTTGATTGTAT+AGG + chr8.2:47829252-47829271 MS.gene032304:intron 25.0%
! GTCATTGAAATAGATAATCA+CGG - chr8.2:47829921-47829940 None:intergenic 25.0%
! GTCATTTGATTAAGGATATT+GGG - chr8.2:47829422-47829441 None:intergenic 25.0%
! TGTGATTATGACAATATTTC+AGG + chr8.2:47828763-47828782 MS.gene032304:intron 25.0%
! TTAAATGAAGAAATCACAAG+AGG - chr8.2:47827635-47827654 None:intergenic 25.0%
!! GAAACCTATAAACATTTTTG+AGG - chr8.2:47829601-47829620 None:intergenic 25.0%
!! TCATACTTGTAAGTTTTACA+TGG + chr8.2:47828552-47828571 MS.gene032304:intron 25.0%
!! TGTCATTTGATTAAGGATAT+TGG - chr8.2:47829423-47829442 None:intergenic 25.0%
!! TTTTATAGTCACATATGACT+TGG - chr8.2:47828894-47828913 None:intergenic 25.0%
!!! GGTTACTTTGTTTTTATTGA+AGG + chr8.2:47829358-47829377 MS.gene032304:intron 25.0%
!!! TAATTTTTTCAGATCTCTGT+AGG + chr8.2:47829548-47829567 MS.gene032304:intron 25.0%
AAAGAGGGAATTTCATTATG+AGG + chr8.2:47829517-47829536 MS.gene032304:intron 30.0%
AAGAGGGAATTTCATTATGA+GGG + chr8.2:47829518-47829537 MS.gene032304:intron 30.0%
AATCTGTACAGACTTAGATT+CGG + chr8.2:47829775-47829794 MS.gene032304:intron 30.0%
ACTATAAAATCGACCCAAAA+TGG + chr8.2:47828905-47828924 MS.gene032304:CDS 30.0%
ATATGTCATAACTCATACCA+AGG - chr8.2:47827772-47827791 None:intergenic 30.0%
GAGGATGCAACATTATAATA+TGG - chr8.2:47830419-47830438 None:intergenic 30.0%
GATTATGACAATATTTCAGG+AGG + chr8.2:47828766-47828785 MS.gene032304:intron 30.0%
TATGTAAAGATTTAGAGAGG+TGG - chr8.2:47828600-47828619 None:intergenic 30.0%
TGGACCTCAAAAATGTTTAT+AGG + chr8.2:47829594-47829613 MS.gene032304:intron 30.0%
TTCAAGATTACTTTGGTTGA+TGG + chr8.2:47830691-47830710 MS.gene032304:CDS 30.0%
! TGCCAATTGATTTACAATAG+AGG - chr8.2:47830438-47830457 None:intergenic 30.0%
! TTATTGAAGGCACAATCAAA+GGG + chr8.2:47829371-47829390 MS.gene032304:intron 30.0%
! TTTATTGAAGGCACAATCAA+AGG + chr8.2:47829370-47829389 MS.gene032304:intron 30.0%
!! CTTTCTTTAATAGGCATTGA+TGG + chr8.2:47829111-47829130 MS.gene032304:intron 30.0%
!! TTTGGTTGATGGTAAAACAT+TGG + chr8.2:47830702-47830721 MS.gene032304:CDS 30.0%
!!! TGCGAATTTTTTTACTTGTG+TGG - chr8.2:47830321-47830340 None:intergenic 30.0%
ACTCTCGTTCAAGATTACTT+TGG + chr8.2:47830684-47830703 MS.gene032304:CDS 35.0%
ATGATGATACCTTTCATGCT+GGG + chr8.2:47828800-47828819 MS.gene032304:CDS 35.0%
GGGTCAATCAAATTTCAACA+TGG + chr8.2:47830525-47830544 MS.gene032304:CDS 35.0%
GTTACCATTGCACAGAAATT+GGG + chr8.2:47830621-47830640 MS.gene032304:CDS 35.0%
GTTTGGAGGACAAAAATTTG+TGG - chr8.2:47828962-47828981 None:intergenic 35.0%
TAATCCACTCAAATTGGTAC+CGG + chr8.2:47830370-47830389 MS.gene032304:intron 35.0%
TAATCTTGAACGAGAGTCTT+TGG - chr8.2:47830681-47830700 None:intergenic 35.0%
TATGATGATACCTTTCATGC+TGG + chr8.2:47828799-47828818 MS.gene032304:CDS 35.0%
TCAATGTTGTGTCTTCCAAT+TGG + chr8.2:47830731-47830750 MS.gene032304:CDS 35.0%
TGTTTGTCCAAATCTCCAAT+TGG - chr8.2:47830749-47830768 None:intergenic 35.0%
TTACCATTGCACAGAAATTG+GGG + chr8.2:47830622-47830641 MS.gene032304:CDS 35.0%
TTCTACCTTCTATAAAGGAC+AGG - chr8.2:47829081-47829100 None:intergenic 35.0%
TTGTTCCTGTCCTTTATAGA+AGG + chr8.2:47829073-47829092 MS.gene032304:CDS 35.0%
! ATGGTGATGATAACAAACGT+TGG + chr8.2:47830544-47830563 MS.gene032304:CDS 35.0%
! TATTGAAGGCACAATCAAAG+GGG + chr8.2:47829372-47829391 MS.gene032304:intron 35.0%
! TTTTGTCCTCCAAACAATCA+TGG + chr8.2:47828967-47828986 MS.gene032304:CDS 35.0%
!!! GACTTCTAAGACACCATTTT+GGG - chr8.2:47828921-47828940 None:intergenic 35.0%
AATATTTCAGGAGGAGCTTG+TGG + chr8.2:47828775-47828794 MS.gene032304:intron 40.0%
ACAGAAATTGGGGAGCTAAT+TGG + chr8.2:47830632-47830651 MS.gene032304:CDS 40.0%
ACTACACTCGTACTACTAGT+TGG + chr8.2:47829574-47829593 MS.gene032304:intron 40.0%
AGAATCCACGTTGAAGCTAA+CGG + chr8.2:47827695-47827714 MS.gene032304:CDS 40.0%
AGGAGTTAGGTTCACATTGA+AGG + chr8.2:47830504-47830523 MS.gene032304:CDS 40.0%
ATTGCACGACAAGGAAATGA+AGG + chr8.2:47829048-47829067 MS.gene032304:CDS 40.0%
GCATGGACATATCAAAGTGA+TGG - chr8.2:47829014-47829033 None:intergenic 40.0%
GGAGTTAGGTTCACATTGAA+GGG + chr8.2:47830505-47830524 MS.gene032304:CDS 40.0%
GGTTACCATTGCACAGAAAT+TGG + chr8.2:47830620-47830639 MS.gene032304:CDS 40.0%
GTGATGATAACAAACGTTGG+TGG + chr8.2:47830547-47830566 MS.gene032304:CDS 40.0%
GTGTCTTCCAATTGGAGATT+TGG + chr8.2:47830739-47830758 MS.gene032304:CDS 40.0%
! AGCACTATGCTTTTCAGAGA+TGG + chr8.2:47828847-47828866 MS.gene032304:CDS 40.0%
! ATATCAAAGTGATGGTGTGG+AGG - chr8.2:47829006-47829025 None:intergenic 40.0%
! GACATATCAAAGTGATGGTG+TGG - chr8.2:47829009-47829028 None:intergenic 40.0%
! TATGCTTTTCAGAGATGGTG+AGG + chr8.2:47828852-47828871 MS.gene032304:CDS 40.0%
!!! CGACTTCTAAGACACCATTT+TGG - chr8.2:47828922-47828941 None:intergenic 40.0%
AACGCTCATGCAACGTTCTA+TGG + chr8.2:47827725-47827744 MS.gene032304:CDS 45.0%
AATCAAAGTCCAACCAGCCT+TGG + chr8.2:47827752-47827771 MS.gene032304:CDS 45.0%
AATGCGAAAGAAAGCAGGCA+TGG - chr8.2:47829031-47829050 None:intergenic 45.0%
ACGCTCATGCAACGTTCTAT+GGG + chr8.2:47827726-47827745 MS.gene032304:CDS 45.0%
AGGGTAGCATGCAAAAGAAG+AGG + chr8.2:47830481-47830500 MS.gene032304:intron 45.0%
CACCATCCTCCATGATTGTT+TGG - chr8.2:47828979-47828998 None:intergenic 45.0%
CATCCTCCATGATTGTTTGG+AGG - chr8.2:47828976-47828995 None:intergenic 45.0%
CTCCAAACAATCATGGAGGA+TGG + chr8.2:47828974-47828993 MS.gene032304:CDS 45.0%
GATACCTTTCATGCTGGGTT+TGG + chr8.2:47828805-47828824 MS.gene032304:CDS 45.0%
GCAAAAGAAGAGGAGGAGTT+AGG + chr8.2:47830491-47830510 MS.gene032304:CDS 45.0%
GGTTGTCACAAAGACACTAC+TGG + chr8.2:47830289-47830308 MS.gene032304:intron 45.0%
GTAGCATGCAAAAGAAGAGG+AGG + chr8.2:47830484-47830503 MS.gene032304:CDS 45.0%
GTCATAACTCATACCAAGGC+TGG - chr8.2:47827768-47827787 None:intergenic 45.0%
TAACTCATACCAAGGCTGGT+TGG - chr8.2:47827764-47827783 None:intergenic 45.0%
TGGATAAGAGGTTCCAGAAG+TGG + chr8.2:47830592-47830611 MS.gene032304:CDS 45.0%
TGTCCTCCAAACAATCATGG+AGG + chr8.2:47828970-47828989 MS.gene032304:CDS 45.0%
TTCAGAGATGGTGAGGTTTG+TGG + chr8.2:47828859-47828878 MS.gene032304:CDS 45.0%
TTCTTTCGCATTGCACGACA+AGG + chr8.2:47829039-47829058 MS.gene032304:CDS 45.0%
! AAGGCTGGTTGGACTTTGAT+TGG - chr8.2:47827753-47827772 None:intergenic 45.0%
! ATAAAGGGGGTGTGGATAAG+AGG + chr8.2:47830580-47830599 MS.gene032304:CDS 45.0%
! TCAAATTGGTACCGGAGAGT+AGG + chr8.2:47830378-47830397 MS.gene032304:intron 45.0%
!! ATAACAAACGTTGGTGGCAG+CGG + chr8.2:47830553-47830572 MS.gene032304:CDS 45.0%
!! TAAAATTAAATAATTAACTT+TGG + chr8.2:47828511-47828530 MS.gene032304:intron 5.0%
!!! TTTTTTATCTATAATTTTAT+TGG + chr8.2:47827831-47827850 MS.gene032304:intron 5.0%
CACACCAAACCCAGCATGAA+AGG - chr8.2:47828812-47828831 None:intergenic 50.0%
CGCTCATGCAACGTTCTATG+GGG + chr8.2:47827727-47827746 MS.gene032304:CDS 50.0%
CGTGCAATGCGAAAGAAAGC+AGG - chr8.2:47829036-47829055 None:intergenic 50.0%
CTCTCCGGTACCAATTTGAG+TGG - chr8.2:47830377-47830396 None:intergenic 50.0%
GCTCCCCAATTTCTGTGCAA+TGG - chr8.2:47830628-47830647 None:intergenic 50.0%
GGCAAAGCAGTGCAGATCTA+AGG + chr8.2:47830653-47830672 MS.gene032304:CDS 50.0%
GGTGGCAGCGGTGATATAAA+GGG + chr8.2:47830565-47830584 MS.gene032304:CDS 50.0%
GTGGCAGCGGTGATATAAAG+GGG + chr8.2:47830566-47830585 MS.gene032304:CDS 50.0%
TCCACGTTGAAGCTAACGGT+TGG + chr8.2:47827699-47827718 MS.gene032304:CDS 50.0%
TGGCAGCGGTGATATAAAGG+GGG + chr8.2:47830567-47830586 MS.gene032304:CDS 50.0%
TGGTAACCAAGCTCCACTTC+TGG - chr8.2:47830608-47830627 None:intergenic 50.0%
TGGTGGCAGCGGTGATATAA+AGG + chr8.2:47830564-47830583 MS.gene032304:CDS 50.0%
GAGGTTCCAGAAGTGGAGCT+TGG + chr8.2:47830599-47830618 MS.gene032304:CDS 55.0%
GCGGTGATATAAAGGGGGTG+TGG + chr8.2:47830572-47830591 MS.gene032304:CDS 55.0%
TCCTACTCGCTCCTACTCTC+CGG - chr8.2:47830392-47830411 None:intergenic 55.0%
! GCCAACCGTTAGCTTCAACG+TGG - chr8.2:47827703-47827722 None:intergenic 55.0%
ACCGGAGAGTAGGAGCGAGT+AGG + chr8.2:47830388-47830407 MS.gene032304:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 47827626 47830795 47827626 ID=MS.gene032304
chr8.2 mRNA 47827626 47830795 47827626 ID=MS.gene032304.t1;Parent=MS.gene032304
chr8.2 exon 47827626 47827773 47827626 ID=MS.gene032304.t1.exon1;Parent=MS.gene032304.t1
chr8.2 CDS 47827626 47827773 47827626 ID=cds.MS.gene032304.t1;Parent=MS.gene032304.t1
chr8.2 exon 47828785 47829094 47828785 ID=MS.gene032304.t1.exon2;Parent=MS.gene032304.t1
chr8.2 CDS 47828785 47829094 47828785 ID=cds.MS.gene032304.t1;Parent=MS.gene032304.t1
chr8.2 exon 47830483 47830795 47830483 ID=MS.gene032304.t1.exon3;Parent=MS.gene032304.t1
chr8.2 CDS 47830483 47830795 47830483 ID=cds.MS.gene032304.t1;Parent=MS.gene032304.t1
Gene Sequence

>MS.gene032304

ATGGGACCTCTTGTGATTTCTTCATTTAATTTTATTTGGTGTCTTTTATTATTGGTTTTATGTGCAGTTAGAATCCACGTTGAAGCTAACGGTTGGCTTAACGCTCATGCAACGTTCTATGGGGCCAATCAAAGTCCAACCAGCCTTGGTATGAGTTATGACATATTTAAGTTTTAGAGATGTCAAAATACATATCAACTTTTTATTTTTTATCTATAATTTTATTGGCGTTTCAATTTTATCTATAATTATGGTTTAGATATTAACTAGTTTACATATATATAATAAGTTTATAGTTTTAAATTTATCAAGAAGGTATAAATGCGAGACTTTGAAAATTATGATGTAAAGTATAATTGTACCTTTTTATTTTAGGGATATAATTGTACCTTATATGCGTGTAAAAATTATTTGAAATTGTTCTTTAGTGAAAACAAATTGACAAAGCTCGTTAGTAAAAATTGTTAAAACAACTTTATGTGTAATAAGTATATAAACATGTCTTGCTAAAAAAAAGTTTATAACATGTCAAGCATAATATATATGTAATTACCCCTTTTTATTGAGAAGTATTATTTGTATAACCATTTTTTTTACAACTTTTTCTCATATTCACATTATGTTTTTATTTTTTCTCTCTATAATTTTAGTTTTTGTGTCAATACCTCTTTTTCTTTGTAAATTTATGGTTGTCTCATAAGTTGTCATAGAAAAGATTGTTCAAATAACACAACTTTTTATGTAAGCTTGCATCATCATCATCATGTGAAAAAGAAACATAATAATATTAATAATAATAATAATAATAATAATAATACTAATAATAATATTAATATTAATATTAATAGTAATAAAATTCAACATGGAGAAAATCTTTATTATATTTAAAATTAAATAATTAACTTTGGATACACTCAATGCATAGATCATACTTGTAAGTTTTACATGGATCCAAAAATATTTTATATAGACCACCTCTCTAAATCTTTACATAAATCTAAAATGATTTGTTGTGTTATCAACATGCATCAATATTGATACCTTATATATTCTAATCTTAACTAATCATTCATGATTTGATTCCATTTGTTTAATATTAAGAACCTTCTGATAATTTAATATAACATTAATCTTCTTTGTGATTATGACAATATTTCAGGAGGAGCTTGTGGTTATGATGATACCTTTCATGCTGGGTTTGGTGTGAACACAGCAGCAGTGAGCACTATGCTTTTCAGAGATGGTGAGGTTTGTGGTGCTTGCTACCAAGTCATATGTGACTATAAAATCGACCCAAAATGGTGTCTTAGAAGTCGAAGTGTTACAGTAACTGCCACAAATTTTTGTCCTCCAAACAATCATGGAGGATGGTGTGATCCTCCACACCATCACTTTGATATGTCCATGCCTGCTTTCTTTCGCATTGCACGACAAGGAAATGAAGGCATTGTTCCTGTCCTTTATAGAAGGTAGAATAAAACATATCTTTCTTTAATAGGCATTGATGGTGCATTCTTTGTTACAAATTTGAAATTAAATAGTTACTTTAGACACATGCATAGAGAGCATCTTGAAAGTTTTATACAAATTTAGATATTTTTATAGAGCTGGAAATTTTACATGAATCTGAAATTACTTGATTGTATAGGATGCATCAAGTTTGACATTGTTTAATAAAAAACTAACAACCGAAAAAGAAATCACTATTTTTTATAAATAAAAAATCTGATGAGGTTACTTTGTTTTTATTGAAGGCACAATCAAAGGGGCGTGAATAAGCATCTCATTTCAAACCCAATATCCTTAATCAAATGACAAACAAATAATTAGCATTATAATAAAGTGTGTGTATATAAAATAATTAAGAAACATATCCCTTTATATGATACATAAAGAGGGAATTTCATTATGAGGGAATTTCATAATTTTTTCAGATCTCTGTAGGAGCACTACACTCGTACTACTAGTTGGACCTCAAAAATGTTTATAGGTTTCATTAAAGGAAAAAAAGATTTATAGGTGGACTTTACTATTATAGTAAATTTTAATAAAATGTTACATCTCTTTTTCTTTTTTTATTGTAAAATCCACTATTTAACTAAAAATGTTAATAATTCATATCTGTACAGACTTTTTTTTTTTTTGACAAAATCTGTACAGACTTAGATTCGGTTGTTATGAATAAAACTGTAAGATATAAATTATAAACAGTATTTTTTATGAAATTTTGGTTTTGATTAAAACTAAAAATATAAATGTTTGTAACATTTAAGTTATAACAACATAAACTAACCGTGATTATCTATTTCAATGACACCAACAATATAACAAAAAAAATATAATTTAAATTCTCTTTTTAATGACACCAACACAATCTTTATTATAGAAAAATTTGTTTAAGGGTATAATTGAGATAATAAAAAAGTAAGTATAAATATATTCATCTAGTTTGTTACACTTTTAATATGATAAAAGGAAAAAACTTAGACATCTATATATTTATATCTTGTTTAATACATTTTTTTAATATTTAAATTTATTCTTATTTATTTGCTACATGTGTTATTTGTATTGAAAATTAAGGACAATTTTGGAAAGAGAAAAAGTCAACTCAAAAGAGCTGAAAAATGTTATTTTCTTTACATATAGTATAGATAATTAACGGTTGTCACAAAGACACTACTGGAGAGATCCACACAAGTAAAAAAATTCGCAAGTCCATATTGTTTTTGAAAATGTTAAATTAATCCACTCAAATTGGTACCGGAGAGTAGGAGCGAGTAGGAGCACCCATATTATAATGTTGCATCCTCTATTGTAAATCAATTGGCACTTATATATACATGTAATTGAACAGGGTAGCATGCAAAAGAAGAGGAGGAGTTAGGTTCACATTGAAGGGTCAATCAAATTTCAACATGGTGATGATAACAAACGTTGGTGGCAGCGGTGATATAAAGGGGGTGTGGATAAGAGGTTCCAGAAGTGGAGCTTGGTTACCATTGCACAGAAATTGGGGAGCTAATTGGCAAAGCAGTGCAGATCTAAGGAACCAAAGACTCTCGTTCAAGATTACTTTGGTTGATGGTAAAACATTGGTGTTTCTCAATGTTGTGTCTTCCAATTGGAGATTTGGACAAACATTTTCATCCCATAATCAATTCTATTAG
Protein sequence

>MS.gene032304.t1

MGPLVISSFNFIWCLLLLVLCAVRIHVEANGWLNAHATFYGANQSPTSLGGACGYDDTFHAGFGVNTAAVSTMLFRDGEVCGACYQVICDYKIDPKWCLRSRSVTVTATNFCPPNNHGGWCDPPHHHFDMSMPAFFRIARQGNEGIVPVLYRRVACKRRGGVRFTLKGQSNFNMVMITNVGGSGDIKGVWIRGSRSGAWLPLHRNWGANWQSSADLRNQRLSFKITLVDGKTLVFLNVVSSNWRFGQTFSSHNQFY