AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033047

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033047.t1 MTR_5g080890 79.224 438 84 5 1 435 1 434 0.0 689
MS.gene033047.t1 MTR_5g080960 78.995 438 85 5 1 435 1 434 0.0 686
MS.gene033047.t1 MTR_8g096990 77.752 436 92 2 1 435 1 432 0.0 677
MS.gene033047.t1 MTR_8g038280 73.394 436 115 1 1 435 1 436 0.0 645
MS.gene033047.t1 MTR_5g044770 72.477 436 114 3 1 435 1 431 0.0 629
MS.gene033047.t1 MTR_8g038250 72.172 442 90 4 1 435 1 416 0.0 619
MS.gene033047.t1 MTR_6g090290 71.330 436 123 2 1 434 1 436 0.0 607
MS.gene033047.t1 MTR_8g038310 63.532 436 134 5 1 434 1 413 0.0 540
MS.gene033047.t1 MTR_4g109760 69.266 436 96 6 1 435 1 399 0.0 539
MS.gene033047.t1 MTR_3g028120 51.313 419 191 6 26 435 25 439 1.17e-145 422
MS.gene033047.t1 MTR_6g088760 53.865 414 177 8 1 405 1 409 5.58e-141 413
MS.gene033047.t1 MTR_5g038360 49.099 444 208 8 1 434 1 436 2.20e-139 406
MS.gene033047.t1 MTR_2g067830 53.528 411 178 7 32 434 1 406 7.73e-138 401
MS.gene033047.t1 MTR_0024s0040 50.360 417 188 8 30 434 1 410 3.09e-129 379
MS.gene033047.t1 MTR_2g105300 50.490 408 167 8 32 431 1 381 4.17e-125 368
MS.gene033047.t1 MTR_5g044570 43.722 446 204 10 1 434 1 411 3.29e-118 351
MS.gene033047.t1 MTR_0036s0070 42.955 440 220 10 10 434 3 426 6.72e-113 338
MS.gene033047.t1 MTR_0036s0050 42.921 445 222 10 10 434 3 435 5.71e-111 333
MS.gene033047.t1 MTR_0036s0110 40.879 455 235 10 1 434 1 442 2.09e-104 317
MS.gene033047.t1 MTR_5g008070 42.043 421 218 10 21 433 23 425 2.15e-96 296
MS.gene033047.t1 MTR_2g059490 46.089 358 180 8 86 435 59 411 1.51e-92 286
MS.gene033047.t1 MTR_2g059460 41.568 421 198 12 23 434 25 406 3.58e-89 276
MS.gene033047.t1 MTR_5g008150 40.284 422 207 11 22 434 27 412 1.36e-87 273
MS.gene033047.t1 MTR_5g008110 38.215 437 222 11 5 434 20 415 3.61e-85 266
MS.gene033047.t1 MTR_3g097150 36.526 449 251 12 7 435 15 449 7.68e-82 259
MS.gene033047.t1 MTR_1g060750 36.700 406 215 10 40 434 2 376 5.84e-75 239
MS.gene033047.t1 MTR_4g045857 40.252 318 150 9 2 314 6 288 2.73e-62 203
MS.gene033047.t1 MTR_5g008090 38.742 302 169 8 141 434 21 314 3.02e-57 191
MS.gene033047.t1 MTR_1g115210 33.562 438 251 17 16 432 68 486 5.97e-55 190
MS.gene033047.t1 MTR_1g039270 36.088 363 200 13 83 432 126 469 1.27e-54 188
MS.gene033047.t1 MTR_7g117430 33.568 426 244 14 28 432 77 484 5.84e-54 187
MS.gene033047.t1 MTR_2g028190 37.017 362 206 12 83 432 100 451 3.57e-52 181
MS.gene033047.t1 MTR_6g088765 43.333 240 94 5 161 397 5 205 7.92e-49 166
MS.gene033047.t1 MTR_3g046300 32.905 389 237 10 66 435 78 461 2.26e-46 166
MS.gene033047.t1 MTR_5g091130 34.367 387 225 12 71 435 177 556 6.49e-46 167
MS.gene033047.t1 MTR_3g053710 33.880 366 212 11 83 432 115 466 4.46e-44 160
MS.gene033047.t1 MTR_6g085010 33.428 353 211 12 86 432 157 491 1.37e-43 159
MS.gene033047.t1 MTR_1g111350 33.240 358 212 12 83 432 129 467 4.10e-43 157
MS.gene033047.t1 MTR_4g007740 33.333 372 206 12 82 433 129 478 2.45e-42 155
MS.gene033047.t1 MTR_8g103880 31.503 346 213 12 83 420 114 443 2.20e-40 150
MS.gene033047.t1 MTR_2g070430 28.387 465 273 17 5 429 6 450 1.05e-35 137
MS.gene033047.t1 MTR_3g053980 33.242 364 211 12 83 432 140 485 1.03e-34 135
MS.gene033047.t1 MTR_2g055840 28.534 382 234 13 77 432 5 373 2.31e-33 129
MS.gene033047.t1 MTR_3g065160 27.005 374 232 13 85 435 74 429 8.16e-30 120
MS.gene033047.t1 MTR_7g105850 29.075 454 247 20 38 434 32 467 1.82e-29 120
MS.gene033047.t1 MTR_3g068000 26.684 386 222 13 88 432 76 441 7.34e-29 118
MS.gene033047.t1 MTR_4g008110 55.556 117 25 1 160 276 48 137 1.14e-28 110
MS.gene033047.t1 MTR_1g078240 28.982 383 221 16 83 433 67 430 1.42e-28 117
MS.gene033047.t1 MTR_8g094590 28.312 385 216 16 83 435 71 427 4.75e-28 115
MS.gene033047.t1 MTR_1g078240 29.610 385 217 17 83 433 67 431 1.05e-27 115
MS.gene033047.t1 MTR_7g080230 26.244 442 267 17 30 433 11 431 1.85e-27 114
MS.gene033047.t1 MTR_8g094590 31.834 289 154 12 83 356 71 331 3.97e-27 112
MS.gene033047.t1 MTR_8g094590 32.955 264 144 10 83 337 71 310 4.23e-27 110
MS.gene033047.t1 MTR_7g062360 27.907 387 220 16 88 435 62 428 9.48e-27 112
MS.gene033047.t1 MTR_4g095270 27.614 373 224 14 84 435 79 426 4.69e-26 111
MS.gene033047.t1 MTR_4g095270 27.614 373 224 14 84 435 79 426 6.77e-26 110
MS.gene033047.t1 MTR_5g016260 24.648 426 261 18 33 435 46 434 1.56e-25 109
MS.gene033047.t1 MTR_2g063110 26.230 427 256 14 55 432 92 508 1.62e-25 108
MS.gene033047.t1 MTR_5g016260 24.648 426 261 18 33 435 46 434 2.13e-25 109
MS.gene033047.t1 MTR_6g084710 27.041 392 221 17 79 432 186 550 3.67e-25 108
MS.gene033047.t1 MTR_1g078240 30.599 317 177 14 83 375 67 364 2.37e-24 104
MS.gene033047.t1 MTR_5g077850 26.098 387 223 15 85 435 81 440 1.20e-23 102
MS.gene033047.t1 MTR_1g061940 28.467 411 235 19 58 433 59 445 1.85e-23 102
MS.gene033047.t1 MTR_7g021510 26.234 385 235 17 81 433 67 434 2.41e-23 102
MS.gene033047.t1 MTR_8g075090 28.647 377 213 18 86 434 113 461 2.19e-22 99.8
MS.gene033047.t1 MTR_4g056420 28.609 381 222 18 84 433 76 437 2.32e-22 99.4
MS.gene033047.t1 MTR_2g103340 44.348 115 21 1 21 135 34 105 3.40e-22 94.0
MS.gene033047.t1 MTR_6g084710 29.010 293 171 12 79 356 186 456 3.83e-22 99.0
MS.gene033047.t1 MTR_3g096930 25.197 381 232 15 83 435 82 437 4.12e-22 98.6
MS.gene033047.t1 MTR_2g084550 29.319 382 219 16 84 433 76 438 5.31e-22 98.6
MS.gene033047.t1 MTR_1g037630 28.009 432 258 19 26 433 27 429 5.94e-22 97.8
MS.gene033047.t1 MTR_8g075090 28.307 378 215 18 86 435 113 462 1.08e-21 97.4
MS.gene033047.t1 MTR_7g102750 28.460 383 222 20 83 433 79 441 1.27e-21 97.4
MS.gene033047.t1 MTR_4g023650 25.436 401 237 18 71 435 48 422 6.11e-21 94.7
MS.gene033047.t1 MTR_3g014290 29.945 364 212 17 86 433 81 417 1.31e-20 94.0
MS.gene033047.t1 MTR_4g023630 24.060 399 245 15 71 435 48 422 1.12e-19 91.3
MS.gene033047.t1 MTR_4g100990 27.446 419 215 22 86 435 75 473 2.06e-19 90.9
MS.gene033047.t1 MTR_5g012490 26.442 416 216 18 86 432 75 469 3.16e-19 90.1
MS.gene033047.t1 MTR_1g029810 24.869 382 232 17 84 435 56 412 8.40e-19 88.6
MS.gene033047.t1 MTR_1g029810 24.607 382 233 17 84 435 56 412 1.13e-18 88.2
MS.gene033047.t1 MTR_8g075090 28.947 342 195 16 91 408 118 435 1.19e-18 88.2
MS.gene033047.t1 MTR_0945s0010 39.683 126 72 3 313 435 1 125 2.98e-18 82.0
MS.gene033047.t1 MTR_8g039540 26.869 428 269 17 26 433 31 434 3.52e-18 86.7
MS.gene033047.t1 MTR_5g061690 27.624 362 209 19 92 427 108 442 1.28e-17 85.5
MS.gene033047.t1 MTR_3g087780 24.834 451 272 18 28 435 27 453 1.32e-17 85.5
MS.gene033047.t1 MTR_7g102750 28.693 352 200 19 83 403 79 410 1.50e-17 84.7
MS.gene033047.t1 MTR_5g022880 26.400 375 222 15 86 432 113 461 2.39e-17 84.7
MS.gene033047.t1 MTR_5g080330 25.137 366 194 15 102 432 2 322 3.75e-17 83.6
MS.gene033047.t1 MTR_7g080230 26.048 334 198 13 128 433 49 361 5.21e-16 79.7
MS.gene033047.t1 MTR_5g077920 25.661 378 236 13 85 434 68 428 7.20e-16 79.7
MS.gene033047.t1 MTR_3g100500 28.333 360 219 13 82 425 94 430 1.44e-15 79.0
MS.gene033047.t1 MTR_3g006820 23.227 409 271 12 52 435 25 415 4.27e-15 77.0
MS.gene033047.t1 MTR_2g037710 28.899 218 123 9 223 432 12 205 2.74e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033047.t1 AT1G64830 49.153 413 202 4 26 435 24 431 6.67e-134 392
MS.gene033047.t1 AT5G33340 50.847 413 194 6 27 435 30 437 1.41e-129 381
MS.gene033047.t1 AT2G35615 45.045 444 223 8 7 433 5 444 8.06e-114 341
MS.gene033047.t1 AT1G31450 43.341 443 227 9 5 433 10 442 4.18e-104 316
MS.gene033047.t1 AT2G28010 40.046 437 202 12 6 435 11 394 1.62e-88 275
MS.gene033047.t1 AT2G28040 39.450 436 205 12 6 435 11 393 7.59e-85 265
MS.gene033047.t1 AT2G28040 40.144 416 193 11 26 435 19 384 4.12e-84 263
MS.gene033047.t1 AT2G28030 43.514 370 175 10 73 435 48 390 1.96e-81 256
MS.gene033047.t1 AT2G28220 37.727 440 214 12 6 435 11 400 3.06e-77 246
MS.gene033047.t1 AT2G03200 37.156 436 231 14 26 435 43 461 1.23e-76 246
MS.gene033047.t1 AT2G28225 40.751 373 185 10 73 435 54 400 1.59e-75 241
MS.gene033047.t1 AT4G30030 36.267 375 208 14 71 432 63 419 3.49e-54 186
MS.gene033047.t1 AT1G01300 38.333 360 198 13 83 432 139 484 1.86e-53 186
MS.gene033047.t1 AT2G23945 29.654 462 271 15 6 435 12 451 4.81e-52 181
MS.gene033047.t1 AT3G61820 35.279 394 227 15 54 432 103 483 1.95e-49 175
MS.gene033047.t1 AT3G20015 31.057 454 248 17 15 432 46 470 4.59e-49 174
MS.gene033047.t1 AT3G18490 32.949 434 246 17 2 426 96 493 2.10e-47 170
MS.gene033047.t1 AT5G10770 34.240 441 241 16 22 433 54 474 3.17e-47 169
MS.gene033047.t1 AT5G10760 35.854 410 223 16 31 432 87 464 5.94e-46 165
MS.gene033047.t1 AT1G25510 35.083 362 200 14 83 432 145 483 1.20e-45 165
MS.gene033047.t1 AT1G79720 32.892 453 247 17 16 432 51 482 5.49e-44 160
MS.gene033047.t1 AT3G25700 31.075 428 246 16 39 433 39 450 1.42e-43 159
MS.gene033047.t1 AT3G12700 31.840 424 256 17 30 433 51 461 5.75e-43 157
MS.gene033047.t1 AT4G30040 31.279 438 236 21 28 427 17 427 2.91e-41 152
MS.gene033047.t1 AT2G42980 32.828 396 222 14 71 435 145 527 1.29e-40 152
MS.gene033047.t1 AT3G59080 32.234 394 225 14 71 435 155 535 3.90e-40 150
MS.gene033047.t1 AT5G36260 30.789 380 212 17 84 433 76 434 8.22e-36 138
MS.gene033047.t1 AT2G39710 30.607 379 204 16 88 427 67 425 3.39e-34 133
MS.gene033047.t1 AT3G02740 29.948 384 226 16 73 433 77 440 3.42e-32 128
MS.gene033047.t1 AT3G59080 30.334 389 203 13 71 435 155 499 1.60e-31 126
MS.gene033047.t1 AT3G02740 29.365 378 229 15 79 433 3 365 2.03e-31 124
MS.gene033047.t1 AT5G37540 28.496 379 221 14 85 432 79 438 2.32e-31 125
MS.gene033047.t1 AT1G66180 27.559 381 224 15 85 435 71 429 3.79e-31 124
MS.gene033047.t1 AT1G09750 29.310 406 240 15 53 432 64 448 4.46e-30 121
MS.gene033047.t1 AT4G33490 25.598 418 248 15 55 435 23 414 2.34e-28 116
MS.gene033047.t1 AT1G65240 27.487 382 227 15 80 433 68 427 3.58e-28 116
MS.gene033047.t1 AT5G43100 27.346 373 224 14 84 435 74 420 2.54e-27 114
MS.gene033047.t1 AT5G02190 26.753 385 211 16 95 432 82 442 3.83e-27 113
MS.gene033047.t1 AT5G43100 27.200 375 222 16 84 435 74 420 5.20e-27 114
MS.gene033047.t1 AT4G12920 28.165 387 230 14 62 432 33 387 5.76e-27 112
MS.gene033047.t1 AT3G54400 28.650 363 219 14 86 433 88 425 8.89e-27 112
MS.gene033047.t1 AT3G50050 27.273 374 225 14 84 435 91 439 4.36e-26 111
MS.gene033047.t1 AT4G33490 27.729 339 199 13 55 373 20 332 1.57e-24 105
MS.gene033047.t1 AT1G44130 25.547 411 244 16 61 435 18 402 2.60e-24 104
MS.gene033047.t1 AT1G05840 27.225 382 233 14 81 433 74 439 3.22e-24 105
MS.gene033047.t1 AT5G22850 29.211 380 224 17 83 433 78 441 7.80e-24 103
MS.gene033047.t1 AT5G45120 27.778 414 216 18 85 432 82 478 1.77e-23 102
MS.gene033047.t1 AT3G52500 28.051 467 243 22 34 433 28 468 3.87e-23 101
MS.gene033047.t1 AT1G49050 25.000 392 240 18 79 434 197 570 1.13e-21 98.2
MS.gene033047.t1 AT1G49050 24.384 365 225 15 104 434 50 397 4.53e-21 95.1
MS.gene033047.t1 AT2G17760 26.869 428 252 21 38 432 50 449 5.17e-21 95.9
MS.gene033047.t1 AT2G36670 27.513 378 231 17 83 433 97 458 1.76e-20 94.0
MS.gene033047.t1 AT2G36670 27.513 378 231 17 83 433 102 463 6.22e-20 92.4
MS.gene033047.t1 AT1G08210 27.660 376 232 16 83 433 115 475 7.33e-20 92.4
MS.gene033047.t1 AT1G08210 27.660 376 232 16 83 433 129 489 7.44e-20 92.4
MS.gene033047.t1 AT2G36670 27.200 375 230 17 86 433 34 392 7.58e-20 91.7
MS.gene033047.t1 AT2G36670 27.200 375 230 17 86 433 34 392 7.58e-20 91.7
MS.gene033047.t1 AT1G08210 27.660 376 232 16 83 433 81 441 7.72e-20 92.0
MS.gene033047.t1 AT1G77480 24.416 385 231 15 84 435 65 422 4.31e-19 89.4
MS.gene033047.t1 AT1G77480 24.416 385 231 15 84 435 65 422 6.75e-19 89.0
MS.gene033047.t1 AT5G07030 25.959 443 281 17 1 420 24 442 3.51e-18 87.0
MS.gene033047.t1 AT1G08210 25.806 403 232 16 83 433 81 468 4.65e-16 80.5
MS.gene033047.t1 AT5G10080 24.274 482 281 25 5 434 6 455 9.55e-16 79.7
MS.gene033047.t1 AT5G24820 23.919 393 236 12 75 432 35 399 9.03e-15 76.3
MS.gene033047.t1 AT3G51360 26.431 367 220 15 91 432 104 445 1.37e-14 76.3
MS.gene033047.t1 AT3G51360 26.431 367 220 15 91 432 94 435 1.49e-14 75.9
MS.gene033047.t1 AT3G51360 26.431 367 220 15 91 432 10 351 1.66e-14 75.5
MS.gene033047.t1 AT3G25700 28.977 176 105 5 39 201 39 207 4.62e-14 73.6
MS.gene033047.t1 AT3G42550 24.138 377 213 14 76 433 68 390 8.17e-14 73.6
MS.gene033047.t1 AT4G35880 24.828 290 180 11 89 362 110 377 1.30e-12 69.7
MS.gene033047.t1 AT3G51360 27.134 328 193 13 91 396 94 397 1.35e-12 69.7
MS.gene033047.t1 AT3G51360 27.134 328 193 13 91 396 104 407 1.70e-12 69.3
MS.gene033047.t1 AT3G51360 27.063 303 179 11 91 375 94 372 3.13e-12 68.6
MS.gene033047.t1 AT3G51360 27.063 303 179 11 91 375 104 382 3.50e-12 68.2
MS.gene033047.t1 AT4G16563 25.862 406 215 19 104 435 101 494 1.73e-11 66.2
MS.gene033047.t1 AT3G51340 23.288 365 232 15 91 432 108 447 3.33e-11 65.5
MS.gene033047.t1 AT3G51330 24.728 368 230 16 91 432 107 453 8.72e-11 64.3
MS.gene033047.t1 AT3G51330 24.728 368 230 16 91 432 107 453 9.46e-11 63.9

Find 84 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGTTTGACTCACTGTTTA+AGG 0.161660 8.2:+16196775 None:intergenic
GGAAATGCTGCTGAGGTTTC+CGG 0.213900 8.2:-16196734 MS.gene033047:CDS
TCTCAAACTGGTGCCATCTT+TGG 0.229990 8.2:-16196317 MS.gene033047:CDS
TTTAAAATGTGCGGTGATTA+GGG 0.231498 8.2:+16196418 None:intergenic
CTTTAAAATGTGCGGTGATT+AGG 0.242187 8.2:+16196417 None:intergenic
GGATTGTAGAGTGGTGATTT+TGG 0.257821 8.2:+16197403 None:intergenic
GCAATACCATAAAATTTAAA+TGG 0.295214 8.2:+16197229 None:intergenic
GATAATACTGTGTCGTTTCA+TGG 0.321598 8.2:-16196908 MS.gene033047:CDS
ATGAGATACGCACCAGTATC+AGG 0.339411 8.2:+16197274 None:intergenic
CGTGTCTCTAATAACACAAT+TGG 0.344254 8.2:-16196849 MS.gene033047:CDS
GGAAACATCATAATTGATTC+AGG 0.355797 8.2:-16196584 MS.gene033047:CDS
AATGTCAAATAGTAGAAAAC+TGG 0.357495 8.2:+16196671 None:intergenic
TGTGAATATTCTATTAGTTA+TGG 0.366324 8.2:-16197037 MS.gene033047:CDS
CTCAAACTGGTGCCATCTTT+GGG 0.367501 8.2:-16196316 MS.gene033047:CDS
GAAGTTATTGGCGCGGTTGA+TGG 0.369229 8.2:+16197336 None:intergenic
TGGTAAATTCTCTTATTGTT+TGG 0.371059 8.2:-16196813 MS.gene033047:CDS
TAAATCAGCTGTGGCAGCTT+TGG 0.379417 8.2:-16196519 MS.gene033047:CDS
GGTAACAAAAGAGTAGAGTT+TGG 0.381657 8.2:-16196632 MS.gene033047:CDS
TGGTGCCATCTTTGGGAACT+TGG 0.386670 8.2:-16196309 MS.gene033047:CDS
CATAGGCCGAATGTTTGATT+AGG 0.389222 8.2:+16196473 None:intergenic
TCATTTCCTAAAACCGTGAT+TGG 0.394071 8.2:-16196941 MS.gene033047:CDS
ATACAGGTAGTGACATTGTT+TGG 0.395178 8.2:-16197204 MS.gene033047:CDS
GGAGATTCATTTCTCACTAT+AGG 0.395756 8.2:+16196692 None:intergenic
CCTAAAACCGTGATTGGTTG+TGG 0.397879 8.2:-16196935 MS.gene033047:CDS
TCCACTGTTATTCCTGATAC+TGG 0.399847 8.2:-16197286 MS.gene033047:CDS
TCAGGAATAACAGTGGATTC+AGG 0.406305 8.2:+16197292 None:intergenic
CTTGGCTCAACAAAATTTGT+TGG 0.415034 8.2:-16196291 MS.gene033047:CDS
TTGAGCCAAGTTCCCAAAGA+TGG 0.430537 8.2:+16196304 None:intergenic
TCTGGTATAGTTGGCCTTGG+AGG 0.436740 8.2:-16196878 MS.gene033047:CDS
GGTATAGTTGGCCTTGGAGG+TGG 0.436885 8.2:-16196875 MS.gene033047:CDS
TCGTTTCATGGCGCAAGCTC+TGG 0.439221 8.2:-16196896 MS.gene033047:CDS
GATTAAACTTAGGAGTGGTT+TGG 0.439457 8.2:+16197149 None:intergenic
AAAGAAAGAGAGGGTAAGAA+AGG 0.440054 8.2:+16197501 None:intergenic
ACACAATTGGGATCATCAAT+AGG 0.440252 8.2:-16196836 MS.gene033047:CDS
AGCTCTGGTATAGTTGGCCT+TGG 0.443123 8.2:-16196881 MS.gene033047:CDS
GCGACGAGCAAACTAAATTT+CGG 0.444525 8.2:-16196755 MS.gene033047:CDS
TGGTGGAGTACCAACTGAAT+AGG 0.444684 8.2:+16197249 None:intergenic
CTGTTTAAGGTAAGCAATAA+CGG 0.450913 8.2:+16196788 None:intergenic
GCTCAACAAAATTTGTTGGT+TGG 0.464745 8.2:-16196287 MS.gene033047:CDS
TGGATTCAGGTGTACTTGTC+AGG 0.472160 8.2:+16197305 None:intergenic
GATGATCCTAATCAAACATT+CGG 0.480032 8.2:-16196479 MS.gene033047:CDS
ACCTTTGTTCAAGTTGCTGA+TGG 0.493097 8.2:-16196365 MS.gene033047:CDS
TTAAGATCGGTATAAACATA+AGG 0.502749 8.2:+16196539 None:intergenic
TCATCAGAAGGTGGTGATGA+AGG 0.505181 8.2:-16196605 MS.gene033047:CDS
AGATGGCACCAGTTTGAGAT+GGG 0.507792 8.2:+16196321 None:intergenic
GGCGCAAGCTCTGGTATAGT+TGG 0.507923 8.2:-16196887 MS.gene033047:CDS
TGCTATAGTGTGACAACAGA+TGG 0.519279 8.2:-16196452 MS.gene033047:CDS
AACAATGTCACTACCTGTAT+CGG 0.531030 8.2:+16197207 None:intergenic
TAGTCTTGAGACTCTTACGT+TGG 0.531120 8.2:-16196987 MS.gene033047:CDS
GTGTCTCTAATAACACAATT+GGG 0.538676 8.2:-16196848 MS.gene033047:CDS
ATCAGTTCCACAACCAATCA+CGG 0.542873 8.2:+16196928 None:intergenic
AAGATGGCACCAGTTTGAGA+TGG 0.546493 8.2:+16196320 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.556371 8.2:-16196653 MS.gene033047:CDS
ACCATCAGCAACTTGAACAA+AGG 0.564974 8.2:+16196364 None:intergenic
CAATTGTGTTATTAGAGACA+CGG 0.570410 8.2:+16196850 None:intergenic
AAGTTCAACACTAAAACCAT+TGG 0.579518 8.2:+16197441 None:intergenic
ATACCATAAAATTTAAATGG+TGG 0.581758 8.2:+16197232 None:intergenic
CTTACGTTGGAATCCACCAC+TGG 0.585380 8.2:-16196974 MS.gene033047:CDS
AACAAAAGAGTAGAGTTTGG+AGG 0.585737 8.2:-16196629 MS.gene033047:CDS
TATCTCTCACAGATTGACAT+AGG 0.588294 8.2:+16197083 None:intergenic
TGGTTGTAACATTGTTCACA+AGG 0.591014 8.2:+16197169 None:intergenic
AGTTGACACAACACTATCAC+CGG 0.591688 8.2:+16196715 None:intergenic
ACAAAAGAGTAGAGTTTGGA+GGG 0.592954 8.2:-16196628 MS.gene033047:CDS
TGATGGATTAAACTTAGGAG+TGG 0.593505 8.2:+16197144 None:intergenic
AAATTTCGGAAATGCTGCTG+AGG 0.597017 8.2:-16196741 MS.gene033047:CDS
GAGACACGGGTCCACCTCCA+AGG 0.600079 8.2:+16196864 None:intergenic
GAGTTTGGAGGGTCATCAGA+AGG 0.601142 8.2:-16196617 MS.gene033047:CDS
TGAAACAGGAAGACCAGTGG+TGG 0.603675 8.2:+16196961 None:intergenic
TTAAAATGTGCGGTGATTAG+GGG 0.604482 8.2:+16196419 None:intergenic
TGCCACAGCTGATTTAAGAT+CGG 0.605369 8.2:+16196526 None:intergenic
TACACTTTAGTACAATCAGT+AGG 0.607700 8.2:+16196230 None:intergenic
TACCGATCTTAAATCAGCTG+TGG 0.611129 8.2:-16196528 MS.gene033047:CDS
ATCTGCACCTTTAAAATGTG+CGG 0.619507 8.2:+16196409 None:intergenic
AATTGTGTTATTAGAGACAC+GGG 0.620720 8.2:+16196851 None:intergenic
CTGTTGTCACACTATAGCAT+AGG 0.623036 8.2:+16196456 None:intergenic
TATCTCATGACCTATTCAGT+TGG 0.630371 8.2:-16197259 MS.gene033047:CDS
AGATCGGTATAAACATAAGG+TGG 0.642421 8.2:+16196542 None:intergenic
TGACATAGGTTAGACGAACA+AGG 0.643051 8.2:+16197097 None:intergenic
GGCGATCAATCACATTCACA+AGG 0.664729 8.2:-16197016 MS.gene033047:CDS
CAATTGGGATCATCAATAGG+TGG 0.673677 8.2:-16196833 MS.gene033047:CDS
TTTGGAGGGTCATCAGAAGG+TGG 0.678751 8.2:-16196614 MS.gene033047:CDS
GATGGCACCAGTTTGAGATG+GGG 0.683431 8.2:+16196322 None:intergenic
ACCAGTATCAGGAATAACAG+TGG 0.728729 8.2:+16197285 None:intergenic
AAATGAAACAGGAAGACCAG+TGG 0.733802 8.2:+16196958 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACCATAAAATTTAAATGG+TGG + chr8.2:16196514-16196533 None:intergenic 20.0%
!! GCAATACCATAAAATTTAAA+TGG + chr8.2:16196517-16196536 None:intergenic 20.0%
!! TGTGAATATTCTATTAGTTA+TGG - chr8.2:16196706-16196725 MS.gene033047:CDS 20.0%
! AAAAAGAACTACTGGAATAA+AGG + chr8.2:16196270-16196289 None:intergenic 25.0%
! AATAAAGGAAAAAGAAAGAG+AGG + chr8.2:16196255-16196274 None:intergenic 25.0%
! AATGTCAAATAGTAGAAAAC+TGG + chr8.2:16197075-16197094 None:intergenic 25.0%
! ATAAAGGAAAAAGAAAGAGA+GGG + chr8.2:16196254-16196273 None:intergenic 25.0%
! TGGTAAATTCTCTTATTGTT+TGG - chr8.2:16196930-16196949 MS.gene033047:CDS 25.0%
! TTAAGATCGGTATAAACATA+AGG + chr8.2:16197207-16197226 None:intergenic 25.0%
!! ACTCCACCATTTAAATTTTA+TGG - chr8.2:16196508-16196527 MS.gene033047:CDS 25.0%
!! AGTTTTCTACTATTTGACAT+TGG - chr8.2:16197074-16197093 MS.gene033047:CDS 25.0%
!!! GATTTTGATGGATTAAACTT+AGG + chr8.2:16196607-16196626 None:intergenic 25.0%
!!! TGTTGATATTTGTTTTGTGT+AGG + chr8.2:16196364-16196383 None:intergenic 25.0%
!!! TTGTAACTTGATGATTTTGA+TGG + chr8.2:16196619-16196638 None:intergenic 25.0%
AAGTTCAACACTAAAACCAT+TGG + chr8.2:16196305-16196324 None:intergenic 30.0%
GATGATCCTAATCAAACATT+CGG - chr8.2:16197264-16197283 MS.gene033047:CDS 30.0%
GTGTCTCTAATAACACAATT+GGG - chr8.2:16196895-16196914 MS.gene033047:CDS 30.0%
TACACTTTAGTACAATCAGT+AGG + chr8.2:16197516-16197535 None:intergenic 30.0%
! AATTGTGTTATTAGAGACAC+GGG + chr8.2:16196895-16196914 None:intergenic 30.0%
! CAATTGTGTTATTAGAGACA+CGG + chr8.2:16196896-16196915 None:intergenic 30.0%
! CTGTTTAAGGTAAGCAATAA+CGG + chr8.2:16196958-16196977 None:intergenic 30.0%
! GGAAACATCATAATTGATTC+AGG - chr8.2:16197159-16197178 MS.gene033047:CDS 30.0%
! TTTAAAATGTGCGGTGATTA+GGG + chr8.2:16197328-16197347 None:intergenic 30.0%
!!! AATTTTTGAAGTTATTGGCG+CGG + chr8.2:16196417-16196436 None:intergenic 30.0%
!!! ACAACACGTTTTTTTGTTGA+AGG + chr8.2:16197488-16197507 None:intergenic 30.0%
!!! CAGGCAATTTTTGAAGTTAT+TGG + chr8.2:16196422-16196441 None:intergenic 30.0%
AAAGAAAGAGAGGGTAAGAA+AGG + chr8.2:16196245-16196264 None:intergenic 35.0%
AACAAAAGAGTAGAGTTTGG+AGG - chr8.2:16197114-16197133 MS.gene033047:CDS 35.0%
AACAATGTCACTACCTGTAT+CGG + chr8.2:16196539-16196558 None:intergenic 35.0%
ACAAAAGAGTAGAGTTTGGA+GGG - chr8.2:16197115-16197134 MS.gene033047:CDS 35.0%
ACACAATTGGGATCATCAAT+AGG - chr8.2:16196907-16196926 MS.gene033047:CDS 35.0%
AGATCGGTATAAACATAAGG+TGG + chr8.2:16197204-16197223 None:intergenic 35.0%
AGCATGAGAAAAAGAACTAC+TGG + chr8.2:16196278-16196297 None:intergenic 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr8.2:16196539-16196558 MS.gene033047:CDS 35.0%
CGTGTCTCTAATAACACAAT+TGG - chr8.2:16196894-16196913 MS.gene033047:CDS 35.0%
CTTGGCTCAACAAAATTTGT+TGG - chr8.2:16197452-16197471 MS.gene033047:CDS 35.0%
GATTAAACTTAGGAGTGGTT+TGG + chr8.2:16196597-16196616 None:intergenic 35.0%
GGAGATTCATTTCTCACTAT+AGG + chr8.2:16197054-16197073 None:intergenic 35.0%
GGTAACAAAAGAGTAGAGTT+TGG - chr8.2:16197111-16197130 MS.gene033047:CDS 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.2:16196484-16196503 MS.gene033047:CDS 35.0%
TATCTCTCACAGATTGACAT+AGG + chr8.2:16196663-16196682 None:intergenic 35.0%
TCATTTCCTAAAACCGTGAT+TGG - chr8.2:16196802-16196821 MS.gene033047:CDS 35.0%
TGGTTGTAACATTGTTCACA+AGG + chr8.2:16196577-16196596 None:intergenic 35.0%
TTAAAATGTGCGGTGATTAG+GGG + chr8.2:16197327-16197346 None:intergenic 35.0%
! CTAATCACCGCACATTTTAA+AGG - chr8.2:16197327-16197346 MS.gene033047:CDS 35.0%
! TTTTATGGTATTGCCGATAC+AGG - chr8.2:16196523-16196542 MS.gene033047:CDS 35.0%
! TTTTCTCATGCTCTAACCAA+TGG - chr8.2:16196286-16196305 MS.gene033047:CDS 35.0%
! TTTTGTGTAGGATTGTAGAG+TGG + chr8.2:16196352-16196371 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.2:16197090-16197109 MS.gene033047:CDS 35.0%
!! ATCTGCACCTTTAAAATGTG+CGG + chr8.2:16197337-16197356 None:intergenic 35.0%
!! CTTTAAAATGTGCGGTGATT+AGG + chr8.2:16197329-16197348 None:intergenic 35.0%
!! GATAATACTGTGTCGTTTCA+TGG - chr8.2:16196835-16196854 MS.gene033047:CDS 35.0%
!! GCTCAACAAAATTTGTTGGT+TGG - chr8.2:16197456-16197475 MS.gene033047:CDS 35.0%
!! TGATGGATTAAACTTAGGAG+TGG + chr8.2:16196602-16196621 None:intergenic 35.0%
!!! ACGGTTTTAGGAAATGAAAC+AGG + chr8.2:16196799-16196818 None:intergenic 35.0%
AAATGAAACAGGAAGACCAG+TGG + chr8.2:16196788-16196807 None:intergenic 40.0%
AAATTTCGGAAATGCTGCTG+AGG - chr8.2:16197002-16197021 MS.gene033047:CDS 40.0%
ACCAGTATCAGGAATAACAG+TGG + chr8.2:16196461-16196480 None:intergenic 40.0%
ACCATCAGCAACTTGAACAA+AGG + chr8.2:16197382-16197401 None:intergenic 40.0%
AGTTGACACAACACTATCAC+CGG + chr8.2:16197031-16197050 None:intergenic 40.0%
ATCAGTTCCACAACCAATCA+CGG + chr8.2:16196818-16196837 None:intergenic 40.0%
CAATTGGGATCATCAATAGG+TGG - chr8.2:16196910-16196929 MS.gene033047:CDS 40.0%
CATAGGCCGAATGTTTGATT+AGG + chr8.2:16197273-16197292 None:intergenic 40.0%
CTGTTGTCACACTATAGCAT+AGG + chr8.2:16197290-16197309 None:intergenic 40.0%
GCGACGAGCAAACTAAATTT+CGG - chr8.2:16196988-16197007 MS.gene033047:CDS 40.0%
TACCGATCTTAAATCAGCTG+TGG - chr8.2:16197215-16197234 MS.gene033047:CDS 40.0%
TCCACTGTTATTCCTGATAC+TGG - chr8.2:16196457-16196476 MS.gene033047:CDS 40.0%
TGACATAGGTTAGACGAACA+AGG + chr8.2:16196649-16196668 None:intergenic 40.0%
TGCTATAGTGTGACAACAGA+TGG - chr8.2:16197291-16197310 MS.gene033047:CDS 40.0%
! GGATTGTAGAGTGGTGATTT+TGG + chr8.2:16196343-16196362 None:intergenic 40.0%
! TAGTCTTGAGACTCTTACGT+TGG - chr8.2:16196756-16196775 MS.gene033047:CDS 40.0%
! TCAGGAATAACAGTGGATTC+AGG + chr8.2:16196454-16196473 None:intergenic 40.0%
! TGCCACAGCTGATTTAAGAT+CGG + chr8.2:16197220-16197239 None:intergenic 40.0%
!! ACCTTTGTTCAAGTTGCTGA+TGG - chr8.2:16197378-16197397 MS.gene033047:CDS 40.0%
AAGATGGCACCAGTTTGAGA+TGG + chr8.2:16197426-16197445 None:intergenic 45.0%
ATGAGATACGCACCAGTATC+AGG + chr8.2:16196472-16196491 None:intergenic 45.0%
CGCGTTTGACTCACTGTTTA+AGG + chr8.2:16196971-16196990 None:intergenic 45.0%
GGCGATCAATCACATTCACA+AGG - chr8.2:16196727-16196746 MS.gene033047:CDS 45.0%
TAAATCAGCTGTGGCAGCTT+TGG - chr8.2:16197224-16197243 MS.gene033047:CDS 45.0%
TGGATTCAGGTGTACTTGTC+AGG + chr8.2:16196441-16196460 None:intergenic 45.0%
TTGAGCCAAGTTCCCAAAGA+TGG + chr8.2:16197442-16197461 None:intergenic 45.0%
! AGATGGCACCAGTTTGAGAT+GGG + chr8.2:16197425-16197444 None:intergenic 45.0%
! CCACAACCAATCACGGTTTT+AGG + chr8.2:16196811-16196830 None:intergenic 45.0%
! CCTAAAACCGTGATTGGTTG+TGG - chr8.2:16196808-16196827 MS.gene033047:CDS 45.0%
! TCATCAGAAGGTGGTGATGA+AGG - chr8.2:16197138-16197157 MS.gene033047:CDS 45.0%
! TGGTGGAGTACCAACTGAAT+AGG + chr8.2:16196497-16196516 None:intergenic 45.0%
!! CTCAAACTGGTGCCATCTTT+GGG - chr8.2:16197427-16197446 MS.gene033047:CDS 45.0%
!! TCTCAAACTGGTGCCATCTT+TGG - chr8.2:16197426-16197445 MS.gene033047:CDS 45.0%
CTTACGTTGGAATCCACCAC+TGG - chr8.2:16196769-16196788 MS.gene033047:CDS 50.0%
GAAGTTATTGGCGCGGTTGA+TGG + chr8.2:16196410-16196429 None:intergenic 50.0%
GAGTTTGGAGGGTCATCAGA+AGG - chr8.2:16197126-16197145 MS.gene033047:CDS 50.0%
GGAAATGCTGCTGAGGTTTC+CGG - chr8.2:16197009-16197028 MS.gene033047:CDS 50.0%
TGAAACAGGAAGACCAGTGG+TGG + chr8.2:16196785-16196804 None:intergenic 50.0%
TTTGGAGGGTCATCAGAAGG+TGG - chr8.2:16197129-16197148 MS.gene033047:CDS 50.0%
! AGCTCTGGTATAGTTGGCCT+TGG - chr8.2:16196862-16196881 MS.gene033047:CDS 50.0%
! GATGGCACCAGTTTGAGATG+GGG + chr8.2:16197424-16197443 None:intergenic 50.0%
! GCTTTTGCCCCATCTCAAAC+TGG - chr8.2:16197414-16197433 MS.gene033047:CDS 50.0%
!! GTGGTGATTTTGGAGAGTCG+CGG + chr8.2:16196333-16196352 None:intergenic 50.0%
!! TCTGGTATAGTTGGCCTTGG+AGG - chr8.2:16196865-16196884 MS.gene033047:CDS 50.0%
!! TGGTGCCATCTTTGGGAACT+TGG - chr8.2:16197434-16197453 MS.gene033047:CDS 50.0%
TCGTTTCATGGCGCAAGCTC+TGG - chr8.2:16196847-16196866 MS.gene033047:CDS 55.0%
! GGCGCAAGCTCTGGTATAGT+TGG - chr8.2:16196856-16196875 MS.gene033047:CDS 55.0%
!! GGTATAGTTGGCCTTGGAGG+TGG - chr8.2:16196868-16196887 MS.gene033047:CDS 55.0%
!! GTGATTTTGGAGAGTCGCGG+TGG + chr8.2:16196330-16196349 None:intergenic 55.0%
GAGACACGGGTCCACCTCCA+AGG + chr8.2:16196882-16196901 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 16196229 16197536 16196229 ID=MS.gene033047
chr8.2 mRNA 16196229 16197536 16196229 ID=MS.gene033047.t1;Parent=MS.gene033047
chr8.2 exon 16196229 16197536 16196229 ID=MS.gene033047.t1.exon1;Parent=MS.gene033047.t1
chr8.2 CDS 16196229 16197536 16196229 ID=cds.MS.gene033047.t1;Parent=MS.gene033047.t1
Gene Sequence

>MS.gene033047

ATGAAAACACATTCCTTTCTTACCCTCTCTTTCTTTTTCCTTTATTCCAGTAGTTCTTTTTCTCATGCTCTAACCAATGGTTTTAGTGTTGAACTTATCCACCGCGACTCTCCAAAATCACCACTCTACAATCCTACACAAAACAAATATCAACATGTTATCAATGCAGCACGTCATTCCATCAACCGCGCCAATAACTTCAAAAATTGCCTGACAAGTACACCTGAATCCACTGTTATTCCTGATACTGGTGCGTATCTCATGACCTATTCAGTTGGTACTCCACCATTTAAATTTTATGGTATTGCCGATACAGGTAGTGACATTGTTTGGCTTCAATGTGAGCCTTGTGAACAATGTTACAACCAAACCACTCCTAAGTTTAATCCATCAAAATCATCAAGTTACAAAAATATTCCTTGTTCGTCTAACCTATGTCAATCTGTGAGAGATACTTCTTGCAATGATCAAAATTTTTGTGAATATTCTATTAGTTATGGCGATCAATCACATTCACAAGGCGATCTTAGTCTTGAGACTCTTACGTTGGAATCCACCACTGGTCTTCCTGTTTCATTTCCTAAAACCGTGATTGGTTGTGGAACTGATAATACTGTGTCGTTTCATGGCGCAAGCTCTGGTATAGTTGGCCTTGGAGGTGGACCCGTGTCTCTAATAACACAATTGGGATCATCAATAGGTGGTAAATTCTCTTATTGTTTGGCGCCGTTATTGCTTACCTTAAACAGTGAGTCAAACGCGACGAGCAAACTAAATTTCGGAAATGCTGCTGAGGTTTCCGGTGATAGTGTTGTGTCAACTCCTATAGTGAGAAATGAATCTCCAGTTTTCTACTATTTGACATTGGAAGCATTTAGTGTTGGTAACAAAAGAGTAGAGTTTGGAGGGTCATCAGAAGGTGGTGATGAAGGAAACATCATAATTGATTCAGGTACAACATTGACGATTTTACCACCTTATGTTTATACCGATCTTAAATCAGCTGTGGCAGCTTTGGTGAAACTAGAGCGTGTTGATGATCCTAATCAAACATTCGGCCTATGCTATAGTGTGACAACAGATGGATACGATTTTCCCCTAATCACCGCACATTTTAAAGGTGCAGATGTTGAGTTGCATTCTATTAGTACCTTTGTTCAAGTTGCTGATGGTATTGTTTGCTTAGCTTTTGCCCCATCTCAAACTGGTGCCATCTTTGGGAACTTGGCTCAACAAAATTTGTTGGTTGGTTATGACCTTCAACAAAAAAACGTGTTGTTTAAGCCTACTGATTGTACTAAAGTGTAA
Protein sequence

>MS.gene033047.t1

MKTHSFLTLSFFFLYSSSSFSHALTNGFSVELIHRDSPKSPLYNPTQNKYQHVINAARHSINRANNFKNCLTSTPESTVIPDTGAYLMTYSVGTPPFKFYGIADTGSDIVWLQCEPCEQCYNQTTPKFNPSKSSSYKNIPCSSNLCQSVRDTSCNDQNFCEYSISYGDQSHSQGDLSLETLTLESTTGLPVSFPKTVIGCGTDNTVSFHGASSGIVGLGGGPVSLITQLGSSIGGKFSYCLAPLLLTLNSESNATSKLNFGNAAEVSGDSVVSTPIVRNESPVFYYLTLEAFSVGNKRVEFGGSSEGGDEGNIIIDSGTTLTILPPYVYTDLKSAVAALVKLERVDDPNQTFGLCYSVTTDGYDFPLITAHFKGADVELHSISTFVQVADGIVCLAFAPSQTGAIFGNLAQQNLLVGYDLQQKNVLFKPTDCTKV