AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034159

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034159.t1 MTR_5g080960 70.185 379 106 5 2 376 58 433 0.0 524
MS.gene034159.t1 MTR_8g096990 69.761 377 109 4 2 376 58 431 0.0 523
MS.gene034159.t1 MTR_5g044770 69.761 377 108 5 2 376 58 430 0.0 519
MS.gene034159.t1 MTR_5g080890 69.657 379 108 5 2 376 58 433 0.0 518
MS.gene034159.t1 MTR_8g038280 67.717 381 114 4 2 376 58 435 1.29e-179 506
MS.gene034159.t1 MTR_8g038250 67.277 382 94 5 2 376 58 415 3.13e-176 496
MS.gene034159.t1 MTR_8g038310 59.524 378 133 4 1 378 58 415 4.77e-158 451
MS.gene034159.t1 MTR_6g090290 62.141 383 135 5 1 376 57 436 1.64e-154 442
MS.gene034159.t1 MTR_4g109760 63.492 378 108 5 2 376 48 398 1.03e-152 436
MS.gene034159.t1 MTR_3g028120 54.400 375 163 6 8 376 66 438 4.97e-134 390
MS.gene034159.t1 MTR_2g067830 55.844 385 157 11 1 376 26 406 4.60e-129 377
MS.gene034159.t1 MTR_5g038360 51.538 390 167 10 1 376 55 436 9.99e-123 362
MS.gene034159.t1 MTR_0024s0040 50.773 388 174 9 1 376 28 410 1.86e-116 345
MS.gene034159.t1 MTR_6g088760 53.429 350 148 8 3 341 59 404 2.03e-114 343
MS.gene034159.t1 MTR_2g105300 52.094 382 148 12 1 373 26 381 2.47e-114 338
MS.gene034159.t1 MTR_5g044570 47.906 382 157 11 3 376 64 411 9.07e-108 322
MS.gene034159.t1 MTR_0036s0070 42.368 380 200 10 3 376 60 426 6.83e-96 293
MS.gene034159.t1 MTR_2g059460 45.308 373 186 11 12 376 44 406 3.36e-95 290
MS.gene034159.t1 MTR_0036s0050 43.005 386 198 11 3 376 60 435 4.47e-95 291
MS.gene034159.t1 MTR_5g008070 42.706 377 205 7 6 375 53 425 1.20e-94 289
MS.gene034159.t1 MTR_2g059490 46.761 355 179 8 30 376 58 410 1.78e-94 290
MS.gene034159.t1 MTR_5g008110 42.368 380 201 10 7 376 44 415 6.37e-92 282
MS.gene034159.t1 MTR_0036s0110 40.665 391 206 10 3 376 61 442 4.61e-91 281
MS.gene034159.t1 MTR_5g008150 42.338 385 203 10 3 376 36 412 1.15e-90 279
MS.gene034159.t1 MTR_1g060750 37.760 384 201 12 3 376 21 376 7.13e-74 234
MS.gene034159.t1 MTR_3g097150 37.047 386 225 10 2 376 70 448 1.85e-70 228
MS.gene034159.t1 MTR_4g045857 46.311 244 123 6 18 254 46 288 4.64e-61 199
MS.gene034159.t1 MTR_5g008090 40.134 299 165 9 87 376 21 314 1.61e-60 198
MS.gene034159.t1 MTR_1g115210 35.028 354 210 11 29 374 145 486 1.52e-55 189
MS.gene034159.t1 MTR_7g117430 36.236 356 206 12 29 374 140 484 3.72e-55 189
MS.gene034159.t1 MTR_1g039270 34.366 355 212 11 29 374 127 469 2.46e-54 186
MS.gene034159.t1 MTR_6g088765 43.983 241 102 5 105 344 3 211 1.60e-50 169
MS.gene034159.t1 MTR_1g111350 35.674 356 201 15 29 374 130 467 5.65e-50 174
MS.gene034159.t1 MTR_3g046300 33.938 386 230 13 12 376 79 460 1.50e-46 165
MS.gene034159.t1 MTR_6g085010 33.143 350 215 11 30 374 156 491 2.11e-46 165
MS.gene034159.t1 MTR_2g028190 33.418 395 223 15 9 374 68 451 6.49e-45 160
MS.gene034159.t1 MTR_5g091130 33.161 386 232 13 12 375 173 554 8.71e-44 159
MS.gene034159.t1 MTR_4g007740 32.687 361 212 15 31 375 133 478 1.25e-41 152
MS.gene034159.t1 MTR_3g053710 33.973 365 208 15 29 374 116 466 2.21e-41 151
MS.gene034159.t1 MTR_4g008110 67.544 114 16 1 103 216 45 137 6.39e-41 141
MS.gene034159.t1 MTR_8g103880 30.114 352 225 11 31 374 117 455 2.19e-37 140
MS.gene034159.t1 MTR_7g080230 30.504 377 217 18 29 375 70 431 1.33e-33 130
MS.gene034159.t1 MTR_2g055840 28.460 383 230 13 22 374 5 373 2.33e-33 128
MS.gene034159.t1 MTR_2g070430 31.099 373 215 15 31 371 88 450 2.18e-32 127
MS.gene034159.t1 MTR_3g068000 28.346 381 219 14 33 374 76 441 9.33e-29 117
MS.gene034159.t1 MTR_3g053980 30.874 366 228 15 21 374 133 485 3.12e-28 115
MS.gene034159.t1 MTR_7g021510 26.893 383 232 18 26 375 67 434 4.21e-27 112
MS.gene034159.t1 MTR_7g105850 29.219 397 214 18 29 376 89 467 4.28e-27 112
MS.gene034159.t1 MTR_1g078240 27.047 403 252 17 2 375 41 430 1.09e-26 111
MS.gene034159.t1 MTR_3g065160 27.926 376 219 14 31 376 75 428 1.63e-25 107
MS.gene034159.t1 MTR_1g078240 27.228 404 251 18 2 375 41 431 1.69e-25 107
MS.gene034159.t1 MTR_7g080230 29.513 349 204 18 54 375 28 361 5.43e-25 105
MS.gene034159.t1 MTR_1g029810 26.471 374 231 14 29 376 56 411 5.52e-25 105
MS.gene034159.t1 MTR_1g029810 26.471 374 231 14 29 376 56 411 7.28e-25 105
MS.gene034159.t1 MTR_5g016260 27.068 399 234 17 3 378 71 435 7.47e-25 106
MS.gene034159.t1 MTR_5g016260 27.068 399 234 17 3 378 71 435 1.20e-24 105
MS.gene034159.t1 MTR_4g023650 26.667 390 243 14 15 376 47 421 1.72e-24 104
MS.gene034159.t1 MTR_2g084550 28.418 373 231 17 29 375 76 438 2.07e-24 104
MS.gene034159.t1 MTR_6g084710 25.974 385 246 14 20 377 181 553 2.27e-24 105
MS.gene034159.t1 MTR_4g095270 27.068 399 234 17 3 378 63 427 6.66e-24 103
MS.gene034159.t1 MTR_4g095270 27.068 399 234 17 3 378 63 427 1.12e-23 103
MS.gene034159.t1 MTR_7g102750 27.224 371 237 16 29 375 80 441 1.68e-23 102
MS.gene034159.t1 MTR_0945s0010 41.600 125 69 3 253 375 1 123 7.51e-23 94.4
MS.gene034159.t1 MTR_5g061690 29.345 351 198 19 37 362 108 433 1.16e-22 99.8
MS.gene034159.t1 MTR_4g023630 24.936 393 246 14 15 376 47 421 1.71e-22 98.6
MS.gene034159.t1 MTR_4g100990 26.859 417 215 18 30 375 74 471 2.30e-22 98.6
MS.gene034159.t1 MTR_1g078240 27.596 337 209 14 2 316 41 364 4.42e-22 97.1
MS.gene034159.t1 MTR_4g056420 28.150 373 231 21 29 375 76 437 4.44e-22 97.8
MS.gene034159.t1 MTR_1g061940 27.346 373 236 17 29 375 82 445 5.32e-21 94.7
MS.gene034159.t1 MTR_2g063110 24.685 397 243 16 29 374 117 508 6.27e-21 94.7
MS.gene034159.t1 MTR_5g012490 27.206 408 224 19 30 375 74 470 7.85e-21 94.0
MS.gene034159.t1 MTR_7g062360 27.154 383 219 16 33 374 62 425 9.23e-21 93.6
MS.gene034159.t1 MTR_6g084710 26.421 299 193 11 20 308 181 462 1.11e-20 94.0
MS.gene034159.t1 MTR_5g022880 27.624 362 221 16 36 374 118 461 1.30e-20 93.6
MS.gene034159.t1 MTR_3g014290 29.014 355 224 15 31 375 81 417 2.41e-20 92.4
MS.gene034159.t1 MTR_7g102750 28.662 314 195 14 29 322 80 384 2.48e-20 92.4
MS.gene034159.t1 MTR_1g037630 29.014 355 224 15 31 375 93 429 3.34e-20 92.0
MS.gene034159.t1 MTR_8g075090 28.184 369 212 21 36 376 118 461 4.34e-20 92.0
MS.gene034159.t1 MTR_8g039540 28.169 355 228 15 31 375 97 434 9.00e-20 90.9
MS.gene034159.t1 MTR_8g075090 28.065 367 211 21 36 374 118 459 2.66e-19 89.4
MS.gene034159.t1 MTR_8g094590 30.078 256 153 9 29 276 72 309 5.81e-19 87.0
MS.gene034159.t1 MTR_3g087780 28.302 371 207 22 37 374 106 450 1.06e-18 87.8
MS.gene034159.t1 MTR_5g077850 25.594 379 228 15 31 376 82 439 3.79e-18 85.9
MS.gene034159.t1 MTR_8g075090 28.746 327 189 19 36 338 118 424 1.35e-17 84.3
MS.gene034159.t1 MTR_5g061690 29.492 295 168 17 84 362 24 294 2.39e-17 83.2
MS.gene034159.t1 MTR_5g080330 26.331 357 196 16 48 374 3 322 7.97e-15 75.9
MS.gene034159.t1 MTR_3g006820 23.785 391 254 17 15 376 39 414 9.62e-14 72.4
MS.gene034159.t1 MTR_3g087780 28.308 325 185 20 73 374 9 308 9.77e-14 72.4
MS.gene034159.t1 MTR_3g087780 28.000 325 186 19 73 374 45 344 1.91e-13 71.6
MS.gene034159.t1 MTR_2g103340 52.542 59 28 0 42 100 67 125 4.35e-13 67.8
MS.gene034159.t1 MTR_3g100500 29.226 349 211 16 29 361 96 424 6.43e-13 70.1
MS.gene034159.t1 MTR_1g072380 24.365 394 217 19 30 369 56 422 2.04e-12 68.6
MS.gene034159.t1 MTR_1g072450 24.010 404 234 19 20 369 30 414 5.76e-12 67.0
MS.gene034159.t1 MTR_2g037710 27.962 211 118 11 175 374 18 205 8.81e-12 64.3
MS.gene034159.t1 MTR_5g077920 23.733 375 242 13 31 376 69 428 1.08e-11 66.2
MS.gene034159.t1 MTR_2g022460 30.000 230 125 13 160 369 171 384 3.24e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034159.t1 AT1G64830 47.656 384 189 7 1 376 51 430 6.76e-112 333
MS.gene034159.t1 AT5G33340 48.031 381 189 8 1 376 60 436 2.66e-108 325
MS.gene034159.t1 AT2G35615 44.757 391 195 9 3 375 57 444 1.85e-99 302
MS.gene034159.t1 AT1G31450 43.990 391 193 10 3 375 60 442 2.78e-90 279
MS.gene034159.t1 AT2G28010 40.470 383 201 11 1 378 35 395 2.71e-85 264
MS.gene034159.t1 AT2G28040 41.514 383 196 12 1 378 35 394 1.76e-81 254
MS.gene034159.t1 AT2G28040 41.514 383 196 11 1 378 26 385 2.06e-81 254
MS.gene034159.t1 AT2G28030 40.274 365 191 11 19 378 49 391 2.42e-76 241
MS.gene034159.t1 AT2G28220 39.378 386 204 11 2 378 37 401 1.25e-75 239
MS.gene034159.t1 AT2G28225 38.108 370 196 11 19 378 55 401 6.17e-71 228
MS.gene034159.t1 AT2G03200 37.990 408 206 15 1 376 68 460 1.50e-65 215
MS.gene034159.t1 AT1G01300 36.872 358 203 13 29 375 140 485 1.77e-55 189
MS.gene034159.t1 AT3G18490 34.565 379 223 14 6 374 137 500 1.90e-53 184
MS.gene034159.t1 AT1G25510 35.294 357 201 13 29 374 146 483 4.75e-51 177
MS.gene034159.t1 AT4G30030 33.676 389 224 16 1 374 50 419 1.17e-50 175
MS.gene034159.t1 AT3G61820 36.088 363 203 13 29 374 133 483 1.21e-49 174
MS.gene034159.t1 AT3G20015 33.148 359 210 13 29 374 129 470 2.26e-49 173
MS.gene034159.t1 AT2G23945 30.874 366 222 12 31 376 96 450 4.01e-46 164
MS.gene034159.t1 AT1G79720 36.685 368 189 17 31 374 135 482 3.83e-43 156
MS.gene034159.t1 AT2G42980 32.902 386 226 16 16 374 145 524 9.47e-42 154
MS.gene034159.t1 AT5G10770 35.734 361 202 16 29 375 130 474 9.69e-41 150
MS.gene034159.t1 AT5G10760 34.565 379 222 14 4 374 104 464 1.10e-40 149
MS.gene034159.t1 AT3G59080 32.208 385 230 17 16 375 155 533 2.82e-40 149
MS.gene034159.t1 AT4G30040 30.914 372 217 17 19 369 75 427 4.56e-40 147
MS.gene034159.t1 AT5G36260 30.667 375 216 16 29 375 76 434 4.20e-39 145
MS.gene034159.t1 AT3G12700 31.781 365 224 13 29 375 104 461 5.03e-39 145
MS.gene034159.t1 AT3G25700 30.788 406 237 17 9 377 54 452 2.82e-38 143
MS.gene034159.t1 AT3G02740 29.268 369 228 16 29 375 83 440 1.86e-32 127
MS.gene034159.t1 AT3G02740 29.640 361 221 16 37 375 16 365 2.20e-32 126
MS.gene034159.t1 AT2G39710 29.737 380 203 18 33 369 67 425 9.90e-31 122
MS.gene034159.t1 AT3G59080 29.737 380 210 15 16 375 155 497 1.21e-30 122
MS.gene034159.t1 AT3G54400 29.534 386 230 16 11 375 61 425 5.86e-30 119
MS.gene034159.t1 AT1G65240 27.467 375 225 16 29 375 72 427 1.53e-27 113
MS.gene034159.t1 AT5G45120 28.434 415 212 21 28 374 81 478 1.92e-27 113
MS.gene034159.t1 AT5G43100 27.692 390 240 16 7 375 50 418 2.01e-26 110
MS.gene034159.t1 AT5G43100 27.692 390 240 16 7 375 50 418 4.77e-26 110
MS.gene034159.t1 AT5G37540 27.733 375 224 16 31 374 80 438 5.34e-26 108
MS.gene034159.t1 AT1G05840 27.614 373 233 15 29 375 78 439 5.70e-26 109
MS.gene034159.t1 AT1G09750 28.536 403 240 18 1 374 65 448 1.03e-25 108
MS.gene034159.t1 AT1G66180 26.842 380 221 16 31 376 72 428 1.07e-25 107
MS.gene034159.t1 AT4G33490 25.815 399 242 16 12 376 35 413 1.99e-25 107
MS.gene034159.t1 AT5G02190 26.203 374 224 16 40 374 82 442 4.72e-24 103
MS.gene034159.t1 AT2G17760 27.805 410 236 22 2 374 63 449 2.22e-23 102
MS.gene034159.t1 AT4G12920 27.606 355 221 14 31 374 58 387 2.43e-23 100
MS.gene034159.t1 AT5G22850 28.610 374 229 18 29 375 79 441 3.35e-23 101
MS.gene034159.t1 AT1G49050 24.234 359 234 15 48 377 49 398 5.40e-23 99.8
MS.gene034159.t1 AT3G50050 27.937 383 234 15 10 376 82 438 6.06e-23 100
MS.gene034159.t1 AT2G36670 26.287 369 242 14 29 375 98 458 6.50e-23 100
MS.gene034159.t1 AT1G49050 23.873 377 255 14 28 377 200 571 1.11e-22 100
MS.gene034159.t1 AT2G36670 26.593 361 235 14 37 375 111 463 1.42e-22 99.4
MS.gene034159.t1 AT2G36670 26.316 361 236 14 37 375 40 392 1.49e-22 99.0
MS.gene034159.t1 AT2G36670 26.316 361 236 14 37 375 40 392 1.49e-22 99.0
MS.gene034159.t1 AT1G44130 27.250 400 236 17 11 376 23 401 1.97e-22 98.2
MS.gene034159.t1 AT4G33490 28.197 305 181 13 12 298 32 316 1.46e-21 95.5
MS.gene034159.t1 AT1G08210 26.613 372 236 17 29 375 116 475 1.16e-20 94.0
MS.gene034159.t1 AT1G08210 26.613 372 236 17 29 375 82 441 1.18e-20 93.6
MS.gene034159.t1 AT1G08210 26.613 372 236 17 29 375 130 489 1.26e-20 94.0
MS.gene034159.t1 AT1G77480 26.005 373 235 16 29 376 65 421 4.69e-20 91.7
MS.gene034159.t1 AT1G77480 26.005 373 235 16 29 376 65 421 4.94e-20 91.7
MS.gene034159.t1 AT3G42550 24.798 371 215 12 21 375 68 390 1.95e-19 89.7
MS.gene034159.t1 AT5G07030 27.195 353 211 14 31 362 115 442 2.21e-19 89.7
MS.gene034159.t1 AT3G51350 26.216 370 228 16 31 374 102 452 3.62e-19 89.4
MS.gene034159.t1 AT3G51360 27.424 361 221 16 36 374 10 351 1.92e-17 83.6
MS.gene034159.t1 AT3G51360 27.424 361 221 16 36 374 104 445 3.47e-17 83.2
MS.gene034159.t1 AT3G51360 27.424 361 221 16 36 374 94 435 3.70e-17 83.2
MS.gene034159.t1 AT3G52500 27.525 396 223 23 29 375 88 468 4.91e-17 82.8
MS.gene034159.t1 AT1G08210 27.619 315 195 15 29 322 82 384 1.30e-16 81.6
MS.gene034159.t1 AT1G49050 25.357 280 185 11 11 283 197 459 2.71e-16 80.5
MS.gene034159.t1 AT5G24820 23.797 395 262 12 9 378 23 403 2.73e-14 74.3
MS.gene034159.t1 AT3G51360 28.308 325 193 15 36 339 94 399 3.29e-14 73.9
MS.gene034159.t1 AT3G51340 24.793 363 226 16 36 374 108 447 3.73e-14 74.3
MS.gene034159.t1 AT3G51360 28.308 325 193 15 36 339 104 409 3.82e-14 73.9
MS.gene034159.t1 AT5G10080 27.224 371 218 22 37 376 106 455 5.15e-14 73.9
MS.gene034159.t1 AT3G51360 27.458 295 182 11 36 315 94 371 1.25e-13 72.0
MS.gene034159.t1 AT3G51360 27.458 295 182 11 36 315 104 381 1.46e-13 72.0
MS.gene034159.t1 AT3G25700 30.319 188 113 7 9 179 54 240 1.78e-13 71.2
MS.gene034159.t1 AT3G51330 26.503 366 223 17 36 374 107 453 4.77e-13 70.5
MS.gene034159.t1 AT3G51330 26.503 366 223 17 36 374 107 453 5.51e-13 70.5
MS.gene034159.t1 AT3G51360 26.733 303 191 14 85 374 25 309 1.69e-12 68.6
MS.gene034159.t1 AT3G51360 26.733 303 191 14 85 374 25 309 1.69e-12 68.6
MS.gene034159.t1 AT3G51340 23.387 372 232 15 36 374 108 459 2.30e-12 68.6
MS.gene034159.t1 AT3G51340 25.442 283 180 11 36 302 108 375 3.73e-12 67.8

Find 84 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGGTATTTATGATAATT+TGG 0.139054 4.3:-87410955 MS.gene034159:CDS
GGAGAAGATGCTGTAGTTTC+TGG 0.182846 4.3:-87411149 MS.gene034159:CDS
ACCCCGCCATTTAAGTTATA+TGG 0.209903 4.3:-87411635 MS.gene034159:CDS
TCACTCAAGGAAGGCATCTT+TGG 0.217493 4.3:-87410726 MS.gene034159:CDS
TTAAAATGTGCGGTGATTAT+AGG 0.227453 4.3:+87410831 None:intergenic
GGAAAGTTCTCTTATTGTTT+GGG 0.246827 4.3:-87411209 MS.gene034159:CDS
ATCGGGGATCACAGTGGATT+TGG 0.254977 4.3:+87411691 None:intergenic
TGGAAAGTTCTCTTATTGTT+TGG 0.264888 4.3:-87411210 MS.gene034159:CDS
ACAAAACCATATAACTTAAA+TGG 0.265952 4.3:+87411629 None:intergenic
ACAGAATAACAAAGGTTAAA+AGG 0.278658 4.3:+87410876 None:intergenic
GGAATCAGCAGTGGTAAAAT+TGG 0.291369 4.3:-87410934 MS.gene034159:CDS
AGCGTCTCTTATAAATCAAT+TGG 0.304096 4.3:-87411246 MS.gene034159:CDS
TCATTTCCTAAAACTATGAT+AGG 0.304111 4.3:-87411338 MS.gene034159:CDS
TGCTGATGGGGTTGTTTGTT+TGG 0.319991 4.3:-87410763 MS.gene034159:CDS
TGCAAATATACTATTGACTA+TGG 0.344759 4.3:-87411434 MS.gene034159:CDS
GGTAACATCATGATTGATTC+AGG 0.346362 4.3:-87410999 MS.gene034159:CDS
CACTCAAGGAAGGCATCTTT+GGG 0.346432 4.3:-87410725 MS.gene034159:CDS
TCGACAAGCAAACTCAGTTT+TGG 0.354009 4.3:-87411170 MS.gene034159:CDS
AACAAAGTCTACACTCAATA+TGG 0.354389 4.3:+87410621 None:intergenic
AGCTCTGGTGTGGTTGGCCT+TGG 0.357134 4.3:-87411278 MS.gene034159:CDS
CACTCAAAGGGTAGTGTTGA+TGG 0.368567 4.3:-87411020 MS.gene034159:CDS
CAAACAACCCCATCAGCAAC+TGG 0.374630 4.3:+87410768 None:intergenic
TAAACGATTCACACGGTTGA+TGG 0.387426 4.3:+87411739 None:intergenic
ACTGGGACAAAGGTGTTAAT+AGG 0.389857 4.3:+87410786 None:intergenic
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.3:-87411272 MS.gene034159:CDS
ATACAGGTAGTGACATAGTT+TGG 0.395461 4.3:-87411604 MS.gene034159:CDS
ATCACATGGAGATCTTAGTT+TGG 0.404282 4.3:-87411399 MS.gene034159:CDS
TCGGGGATCACAGTGGATTT+GGG 0.412343 4.3:+87411692 None:intergenic
CCTTTGTCCCAGTTGCTGAT+GGG 0.431564 4.3:-87410776 MS.gene034159:CDS
ACCTTTGTCCCAGTTGCTGA+TGG 0.433069 4.3:-87410777 MS.gene034159:CDS
GATTAAACATAGGAGTCGTT+TGG 0.435507 4.3:+87411549 None:intergenic
AACCATATAACTTAAATGGC+GGG 0.444894 4.3:+87411633 None:intergenic
GGCCAACCACACCAGAGCTC+TGG 0.446413 4.3:+87411282 None:intergenic
ATAAGAAACTCACCGTCATC+GGG 0.448901 4.3:+87411674 None:intergenic
AATCAATTGGGTTCATCAAT+CGG 0.452100 4.3:-87411233 MS.gene034159:CDS
GGCCAGAGCTCTGGTGTGGT+TGG 0.453163 4.3:-87411284 MS.gene034159:CDS
ATGCCTTCCTTGAGTGAAGA+TGG 0.457005 4.3:+87410732 None:intergenic
TTTAGCTCAACAGAATTTGT+TGG 0.460799 4.3:-87410700 MS.gene034159:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.3:-87411371 MS.gene034159:CDS
TAGTGTTGGAAACAAAATAG+TGG 0.485317 4.3:-87411054 MS.gene034159:CDS
GCGTCTCTTATAAATCAATT+GGG 0.487687 4.3:-87411245 MS.gene034159:CDS
CATAAGAAACTCACCGTCAT+CGG 0.488061 4.3:+87411673 None:intergenic
TCAATATGGTTTGGTACAAT+CGG 0.489521 4.3:+87410635 None:intergenic
TCATTTGAAGGCCAGAGCTC+TGG 0.493916 4.3:-87411293 MS.gene034159:CDS
CCTAAAACTATGATAGGTTG+TGG 0.495262 4.3:-87411332 MS.gene034159:CDS
TGGCGGGGTACCGATTGAAT+AGG 0.499470 4.3:+87411649 None:intergenic
AGTCTACACTCAATATGGTT+TGG 0.504081 4.3:+87410626 None:intergenic
TTTCTTATGACCTATTCAAT+CGG 0.507430 4.3:-87411659 MS.gene034159:CDS
CCCATCAGCAACTGGGACAA+AGG 0.511669 4.3:+87410776 None:intergenic
CTTTGTCCCAGTTGCTGATG+GGG 0.513410 4.3:-87410775 MS.gene034159:CDS
TTGTAACTTGATGATTTCAA+AGG 0.516263 4.3:+87411527 None:intergenic
TATACTATTGACTATGGTGA+TGG 0.523155 4.3:-87411428 MS.gene034159:CDS
CTTGTCGAGTTAGACGACTC+TGG 0.532316 4.3:+87411185 None:intergenic
TTCCCATCTTCACTCAAGGA+AGG 0.532641 4.3:-87410735 MS.gene034159:CDS
GATGCTGTAGTTTCTGGCCA+TGG 0.536021 4.3:-87411143 MS.gene034159:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.3:-87411068 MS.gene034159:CDS
GGAATTTGAAAAGCACTCAA+AGG 0.542170 4.3:-87411033 MS.gene034159:CDS
AGGTTAAAAGGATCATCAGT+TGG 0.545914 4.3:+87410888 None:intergenic
GAATTTGAAAAGCACTCAAA+GGG 0.551524 4.3:-87411032 MS.gene034159:CDS
GCTCAACAGAATTTGTTGGT+TGG 0.553264 4.3:-87410696 MS.gene034159:CDS
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.3:-87411208 MS.gene034159:CDS
GATTTCAAAGGATTAAACAT+AGG 0.557880 4.3:+87411539 None:intergenic
ACCATATAACTTAAATGGCG+GGG 0.569617 4.3:+87411634 None:intergenic
TATGGTTTGGTACAATCGGT+AGG 0.569939 4.3:+87410639 None:intergenic
TCCACTGTGATCCCCGATGA+CGG 0.573173 4.3:-87411686 MS.gene034159:CDS
AAACCATATAACTTAAATGG+CGG 0.573940 4.3:+87411632 None:intergenic
TGCCTTCCTTGAGTGAAGAT+GGG 0.574486 4.3:+87410733 None:intergenic
TGATAATTTGGAATCAGCAG+TGG 0.583321 4.3:-87410943 MS.gene034159:CDS
TTGATGTAACAGAATAACAA+AGG 0.586188 4.3:+87410868 None:intergenic
GAGACGCTGGTCCATTTCCA+AGG 0.605463 4.3:+87411261 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.3:+87411358 None:intergenic
CTTTGTATAAACGATTCACA+CGG 0.608440 4.3:+87411732 None:intergenic
TGAAGGCCAGAGCTCTGGTG+TGG 0.622062 4.3:-87411288 MS.gene034159:CDS
AAACAACCCCATCAGCAACT+GGG 0.622207 4.3:+87410769 None:intergenic
AAATTATCATAAATACCAGA+TGG 0.626186 4.3:+87410957 None:intergenic
AATTGATTTATAAGAGACGC+TGG 0.633040 4.3:+87411248 None:intergenic
ATCCGCACCTTTAAAATGTG+CGG 0.635428 4.3:+87410821 None:intergenic
TGGTTGTAACAATGTTCACA+AGG 0.636110 4.3:+87411569 None:intergenic
AAGGAGTTGACACAACTCCA+TGG 0.639655 4.3:+87411126 None:intergenic
CAATTGGGTTCATCAATCGG+TGG 0.649649 4.3:-87411230 MS.gene034159:CDS
GGTGATGGATCAATATCACA+TGG 0.658999 4.3:-87411413 MS.gene034159:CDS
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.3:+87411355 None:intergenic
TAAGAAACTCACCGTCATCG+GGG 0.707255 4.3:+87411675 None:intergenic
ACCGTCATCGGGGATCACAG+TGG 0.716584 4.3:+87411685 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAAGTAAAAAATTTGT+GGG + chr4.3:87411297-87411316 None:intergenic 10.0%
!! GTTATAAAGTAAAAAATTTG+TGG + chr4.3:87411298-87411317 None:intergenic 15.0%
!! AAATTATCATAAATACCAGA+TGG + chr4.3:87411430-87411449 None:intergenic 20.0%
!! ACAAAACCATATAACTTAAA+TGG + chr4.3:87410758-87410777 None:intergenic 20.0%
!! ATCTGGTATTTATGATAATT+TGG - chr4.3:87411429-87411448 MS.gene034159:CDS 20.0%
!!! AGTTTTTTTTTTACTACAAG+AGG + chr4.3:87410930-87410949 None:intergenic 20.0%
! AAACCATATAACTTAAATGG+CGG + chr4.3:87410755-87410774 None:intergenic 25.0%
! ACAGAATAACAAAGGTTAAA+AGG + chr4.3:87411511-87411530 None:intergenic 25.0%
! GATTTCAAAGGATTAAACAT+AGG + chr4.3:87410848-87410867 None:intergenic 25.0%
! TCATTTCCTAAAACTATGAT+AGG - chr4.3:87411046-87411065 MS.gene034159:CDS 25.0%
! TTGATGTAACAGAATAACAA+AGG + chr4.3:87411519-87411538 None:intergenic 25.0%
! TTTCTTATGACCTATTCAAT+CGG - chr4.3:87410725-87410744 MS.gene034159:CDS 25.0%
!! TGCAAATATACTATTGACTA+TGG - chr4.3:87410950-87410969 MS.gene034159:CDS 25.0%
!! TTGTAACTTGATGATTTCAA+AGG + chr4.3:87410860-87410879 None:intergenic 25.0%
!!! AAAAATAGAGTGTCATTTGA+AGG - chr4.3:87411079-87411098 MS.gene034159:CDS 25.0%
!!! GTACATTTTTTAGACAAACA+AGG + chr4.3:87410893-87410912 None:intergenic 25.0%
!!! TTATATGGTTTTGTTGATAC+AGG - chr4.3:87410764-87410783 MS.gene034159:CDS 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.3:87411032-87411051 None:intergenic 30.0%
AACCATATAACTTAAATGGC+GGG + chr4.3:87410754-87410773 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+CGG - chr4.3:87411151-87411170 MS.gene034159:CDS 30.0%
AGCGTCTCTTATAAATCAAT+TGG - chr4.3:87411138-87411157 MS.gene034159:CDS 30.0%
CTTTGTATAAACGATTCACA+CGG + chr4.3:87410655-87410674 None:intergenic 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.3:87411176-87411195 MS.gene034159:CDS 30.0%
GCGTCTCTTATAAATCAATT+GGG - chr4.3:87411139-87411158 MS.gene034159:CDS 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.3:87411175-87411194 MS.gene034159:CDS 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.3:87411174-87411193 MS.gene034159:CDS 30.0%
TTAAAATGTGCGGTGATTAT+AGG + chr4.3:87411556-87411575 None:intergenic 30.0%
! AATTGATTTATAAGAGACGC+TGG + chr4.3:87411139-87411158 None:intergenic 30.0%
! ATAATCACCGCACATTTTAA+AGG - chr4.3:87411556-87411575 MS.gene034159:CDS 30.0%
! TAGTGTTGGAAACAAAATAG+TGG - chr4.3:87411330-87411349 MS.gene034159:CDS 30.0%
! TATACTATTGACTATGGTGA+TGG - chr4.3:87410956-87410975 MS.gene034159:CDS 30.0%
! TTTAGCTCAACAGAATTTGT+TGG - chr4.3:87411684-87411703 MS.gene034159:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.3:87411316-87411335 MS.gene034159:CDS 30.0%
!! GAATTTGAAAAGCACTCAAA+GGG - chr4.3:87411352-87411371 MS.gene034159:CDS 30.0%
!! TCAATATGGTTTGGTACAAT+CGG + chr4.3:87411752-87411771 None:intergenic 30.0%
ACCATATAACTTAAATGGCG+GGG + chr4.3:87410753-87410772 None:intergenic 35.0%
AGGTTAAAAGGATCATCAGT+TGG + chr4.3:87411499-87411518 None:intergenic 35.0%
ATACAGGTAGTGACATAGTT+TGG - chr4.3:87410780-87410799 MS.gene034159:CDS 35.0%
ATCACATGGAGATCTTAGTT+TGG - chr4.3:87410985-87411004 MS.gene034159:CDS 35.0%
CCTAAAACTATGATAGGTTG+TGG - chr4.3:87411052-87411071 MS.gene034159:CDS 35.0%
GATTAAACATAGGAGTCGTT+TGG + chr4.3:87410838-87410857 None:intergenic 35.0%
TGGTTGTAACAATGTTCACA+AGG + chr4.3:87410818-87410837 None:intergenic 35.0%
! ACAGTGACAATTTTACCATC+TGG - chr4.3:87411412-87411431 MS.gene034159:CDS 35.0%
! CCACAACCTATCATAGTTTT+AGG + chr4.3:87411055-87411074 None:intergenic 35.0%
! GGTAACATCATGATTGATTC+AGG - chr4.3:87411385-87411404 MS.gene034159:CDS 35.0%
! TGATAATTTGGAATCAGCAG+TGG - chr4.3:87411441-87411460 MS.gene034159:CDS 35.0%
!! GGAATTTGAAAAGCACTCAA+AGG - chr4.3:87411351-87411370 MS.gene034159:CDS 35.0%
!!! TGTGGGTCTTTTTTCACTAA+AGG + chr4.3:87411280-87411299 None:intergenic 35.0%
ATAAGAAACTCACCGTCATC+GGG + chr4.3:87410713-87410732 None:intergenic 40.0%
CATAAGAAACTCACCGTCAT+CGG + chr4.3:87410714-87410733 None:intergenic 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.3:87411013-87411032 MS.gene034159:CDS 40.0%
GGAATCAGCAGTGGTAAAAT+TGG - chr4.3:87411450-87411469 MS.gene034159:CDS 40.0%
TAAACGATTCACACGGTTGA+TGG + chr4.3:87410648-87410667 None:intergenic 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.3:87411029-87411048 None:intergenic 40.0%
! ACCCCGCCATTTAAGTTATA+TGG - chr4.3:87410749-87410768 MS.gene034159:CDS 40.0%
! TCGACAAGCAAACTCAGTTT+TGG - chr4.3:87411214-87411233 MS.gene034159:CDS 40.0%
! TCTTTTCCCATCTTCACTCA+AGG - chr4.3:87411645-87411664 MS.gene034159:CDS 40.0%
!! ACTGGGACAAAGGTGTTAAT+AGG + chr4.3:87411601-87411620 None:intergenic 40.0%
!! ATCCGCACCTTTAAAATGTG+CGG + chr4.3:87411566-87411585 None:intergenic 40.0%
!! GCTCAACAGAATTTGTTGGT+TGG - chr4.3:87411688-87411707 MS.gene034159:CDS 40.0%
!! GGTGATGGATCAATATCACA+TGG - chr4.3:87410971-87410990 MS.gene034159:CDS 40.0%
!! TATGGTTTGGTACAATCGGT+AGG + chr4.3:87411748-87411767 None:intergenic 40.0%
AAACAACCCCATCAGCAACT+GGG + chr4.3:87411618-87411637 None:intergenic 45.0%
AAGGAGTTGACACAACTCCA+TGG + chr4.3:87411261-87411280 None:intergenic 45.0%
ATGCCTTCCTTGAGTGAAGA+TGG + chr4.3:87411655-87411674 None:intergenic 45.0%
CAATTGGGTTCATCAATCGG+TGG - chr4.3:87411154-87411173 MS.gene034159:CDS 45.0%
GGAGAAGATGCTGTAGTTTC+TGG - chr4.3:87411235-87411254 MS.gene034159:CDS 45.0%
TAAGAAACTCACCGTCATCG+GGG + chr4.3:87410712-87410731 None:intergenic 45.0%
TGCCTTCCTTGAGTGAAGAT+GGG + chr4.3:87411654-87411673 None:intergenic 45.0%
TGCTGATGGGGTTGTTTGTT+TGG - chr4.3:87411621-87411640 MS.gene034159:CDS 45.0%
TTCCCATCTTCACTCAAGGA+AGG - chr4.3:87411649-87411668 MS.gene034159:CDS 45.0%
! CACTCAAAGGGTAGTGTTGA+TGG - chr4.3:87411364-87411383 MS.gene034159:CDS 45.0%
!! CACCGCACATTTTAAAGGTG+CGG - chr4.3:87411561-87411580 MS.gene034159:CDS 45.0%
!! CACTCAAGGAAGGCATCTTT+GGG - chr4.3:87411659-87411678 MS.gene034159:CDS 45.0%
!! TCACTCAAGGAAGGCATCTT+TGG - chr4.3:87411658-87411677 MS.gene034159:CDS 45.0%
ACCTTTGTCCCAGTTGCTGA+TGG - chr4.3:87411607-87411626 MS.gene034159:CDS 50.0%
CAAACAACCCCATCAGCAAC+TGG + chr4.3:87411619-87411638 None:intergenic 50.0%
CCTTTGTCCCAGTTGCTGAT+GGG - chr4.3:87411608-87411627 MS.gene034159:CDS 50.0%
CTTGTCGAGTTAGACGACTC+TGG + chr4.3:87411202-87411221 None:intergenic 50.0%
CTTTGTCCCAGTTGCTGATG+GGG - chr4.3:87411609-87411628 MS.gene034159:CDS 50.0%
TCATTTGAAGGCCAGAGCTC+TGG - chr4.3:87411091-87411110 MS.gene034159:CDS 50.0%
! ATCGGGGATCACAGTGGATT+TGG + chr4.3:87410696-87410715 None:intergenic 50.0%
! TCGGGGATCACAGTGGATTT+GGG + chr4.3:87410695-87410714 None:intergenic 50.0%
!! GATGCTGTAGTTTCTGGCCA+TGG - chr4.3:87411241-87411260 MS.gene034159:CDS 50.0%
CCCATCAGCAACTGGGACAA+AGG + chr4.3:87411611-87411630 None:intergenic 55.0%
GAGACGCTGGTCCATTTCCA+AGG + chr4.3:87411126-87411145 None:intergenic 55.0%
TCCACTGTGATCCCCGATGA+CGG - chr4.3:87410698-87410717 MS.gene034159:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.3:87411112-87411131 MS.gene034159:CDS 55.0%
!! TGGCGGGGTACCGATTGAAT+AGG + chr4.3:87410738-87410757 None:intergenic 55.0%
ACCGTCATCGGGGATCACAG+TGG + chr4.3:87410702-87410721 None:intergenic 60.0%
! TGAAGGCCAGAGCTCTGGTG+TGG - chr4.3:87411096-87411115 MS.gene034159:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.3:87411106-87411125 MS.gene034159:CDS 60.0%
GGCCAACCACACCAGAGCTC+TGG + chr4.3:87411105-87411124 None:intergenic 65.0%
! GGCCAGAGCTCTGGTGTGGT+TGG - chr4.3:87411100-87411119 MS.gene034159:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 87410635 87411771 87410635 ID=MS.gene034159
chr4.3 mRNA 87410635 87411771 87410635 ID=MS.gene034159.t1;Parent=MS.gene034159
chr4.3 exon 87410635 87411771 87410635 ID=MS.gene034159.t1.exon1;Parent=MS.gene034159.t1
chr4.3 CDS 87410635 87411771 87410635 ID=cds.MS.gene034159.t1;Parent=MS.gene034159.t1
Gene Sequence

>MS.gene034159

ATGCATCGTTCCATCAACCGTGTGAATCGTTTATACAAAGATTCTCTTACTAACACACCCAAATCCACTGTGATCCCCGATGACGGTGAGTTTCTTATGACCTATTCAATCGGTACCCCGCCATTTAAGTTATATGGTTTTGTTGATACAGGTAGTGACATAGTTTGGCTTCAATGTAAGCCTTGTGAACATTGTTACAACCAAACGACTCCTATGTTTAATCCTTTGAAATCATCAAGTTACAAAAACAATATTCCTTGTTTGTCTAAAAAATGTACGTCTGTTGAATCTACCTCTTGTAGTAAAAAAAAAACTTGCAAATATACTATTGACTATGGTGATGGATCAATATCACATGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACTATGATAGGTTGTGGACGTAAAAATAGAGTGTCATTTGAAGGCCAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCAGCGTCTCTTATAAATCAATTGGGTTCATCAATCGGTGGAAAGTTCTCTTATTGTTTGGGGCCAGAGTCGTCTAACTCGACAAGCAAACTCAGTTTTGGAGAAGATGCTGTAGTTTCTGGCCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCCACAAATTTTTTACTTTATAACATTGAAAGCATTTAGTGTTGGAAACAAAATAGTGGAATTTGAAAAGCACTCAAAGGGTAGTGTTGATGGTAACATCATGATTGATTCAGGTACAACAGTGACAATTTTACCATCTGGTATTTATGATAATTTGGAATCAGCAGTGGTAAAATTGGTTAAACTGAAGCGTGTAAAAAATCCAACTGATGATCCTTTTAACCTTTGTTATTCTGTTACATCAAATAAATATGATTTTCCTATAATCACCGCACATTTTAAAGGTGCGGATGTTAAGTTGCATCCTATTAACACCTTTGTCCCAGTTGCTGATGGGGTTGTTTGTTTGGCTCTTTTCCCATCTTCACTCAAGGAAGGCATCTTTGGGAATTTAGCTCAACAGAATTTGTTGGTTGGTTATGATCTTCAACAAAAAATCGTGTCTTTTAAGCCTACCGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene034159.t1

MHRSINRVNRLYKDSLTNTPKSTVIPDDGEFLMTYSIGTPPFKLYGFVDTGSDIVWLQCKPCEHCYNQTTPMFNPLKSSSYKNNIPCLSKKCTSVESTSCSKKKTCKYTIDYGDGSISHGDLSLETLTLDSTSGSSISFPKTMIGCGRKNRVSFEGQSSGVVGLGNGPASLINQLGSSIGGKFSYCLGPESSNSTSKLSFGEDAVVSGHGVVSTPLVKKDPQIFYFITLKAFSVGNKIVEFEKHSKGSVDGNIMIDSGTTVTILPSGIYDNLESAVVKLVKLKRVKNPTDDPFNLCYSVTSNKYDFPIITAHFKGADVKLHPINTFVPVADGVVCLALFPSSLKEGIFGNLAQQNLLVGYDLQQKIVSFKPTDCTKPY