AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034160.t1 MTR_8g096990 66.897 435 138 5 1 433 1 431 0.0 583
MS.gene034160.t1 MTR_5g080960 66.362 437 139 6 1 433 1 433 0.0 569
MS.gene034160.t1 MTR_5g080890 65.904 437 141 6 1 433 1 433 0.0 564
MS.gene034160.t1 MTR_5g044770 66.279 430 138 6 6 433 6 430 0.0 560
MS.gene034160.t1 MTR_8g038280 65.207 434 141 5 6 433 6 435 0.0 558
MS.gene034160.t1 MTR_8g038250 65.000 440 122 6 1 433 1 415 0.0 555
MS.gene034160.t1 MTR_8g038310 59.540 435 156 4 1 435 1 415 0.0 526
MS.gene034160.t1 MTR_6g090290 61.678 441 156 7 1 433 1 436 1.38e-175 498
MS.gene034160.t1 MTR_4g109760 57.569 436 144 8 1 433 1 398 2.33e-152 438
MS.gene034160.t1 MTR_3g028120 51.566 415 190 7 28 433 26 438 1.37e-139 407
MS.gene034160.t1 MTR_2g067830 54.634 410 173 11 33 433 1 406 6.86e-136 396
MS.gene034160.t1 MTR_6g088760 52.300 413 181 9 1 402 1 408 1.71e-131 389
MS.gene034160.t1 MTR_5g038360 48.394 436 207 11 9 433 8 436 8.74e-129 379
MS.gene034160.t1 MTR_2g105300 51.351 407 163 12 33 430 1 381 8.61e-122 359
MS.gene034160.t1 MTR_0024s0040 47.596 416 199 10 31 433 1 410 2.73e-117 349
MS.gene034160.t1 MTR_5g044570 43.341 443 207 11 3 433 1 411 2.29e-112 336
MS.gene034160.t1 MTR_5g008070 41.388 418 223 10 22 432 23 425 4.32e-98 300
MS.gene034160.t1 MTR_0036s0110 39.207 454 243 14 1 433 1 442 1.95e-97 299
MS.gene034160.t1 MTR_0036s0050 41.050 419 225 11 27 433 27 435 3.47e-97 298
MS.gene034160.t1 MTR_0036s0070 40.000 435 234 12 11 433 7 426 4.26e-97 298
MS.gene034160.t1 MTR_2g059490 41.190 437 213 13 5 432 8 409 3.93e-94 291
MS.gene034160.t1 MTR_2g059460 41.148 418 202 13 24 433 25 406 1.50e-93 288
MS.gene034160.t1 MTR_5g008110 36.782 435 231 9 6 433 18 415 2.76e-87 272
MS.gene034160.t1 MTR_5g008150 38.810 420 214 12 23 433 27 412 3.31e-85 266
MS.gene034160.t1 MTR_1g060750 34.739 403 225 12 41 433 2 376 2.00e-68 222
MS.gene034160.t1 MTR_3g097150 33.810 420 251 11 28 433 42 448 9.05e-66 217
MS.gene034160.t1 MTR_5g008090 39.000 300 167 10 144 433 21 314 7.06e-58 192
MS.gene034160.t1 MTR_4g045857 38.994 318 155 10 3 311 1 288 1.84e-57 191
MS.gene034160.t1 MTR_7g117430 35.014 357 209 13 86 431 140 484 6.58e-51 179
MS.gene034160.t1 MTR_1g115210 30.769 429 271 11 18 431 69 486 5.60e-50 176
MS.gene034160.t1 MTR_6g088765 43.277 238 102 5 162 398 3 208 3.26e-49 167
MS.gene034160.t1 MTR_1g039270 33.239 355 216 11 86 431 127 469 3.43e-49 174
MS.gene034160.t1 MTR_4g008110 77.876 113 4 1 161 273 46 137 1.21e-48 162
MS.gene034160.t1 MTR_1g111350 34.463 354 208 13 86 431 130 467 7.15e-46 165
MS.gene034160.t1 MTR_2g028190 32.432 370 223 14 78 431 93 451 3.19e-40 149
MS.gene034160.t1 MTR_3g046300 33.077 390 228 14 69 433 79 460 1.59e-39 147
MS.gene034160.t1 MTR_6g085010 32.286 350 218 10 87 431 156 491 5.05e-39 147
MS.gene034160.t1 MTR_3g053710 33.884 363 211 15 86 431 116 466 7.69e-38 143
MS.gene034160.t1 MTR_4g007740 31.304 345 208 14 104 432 147 478 2.78e-37 142
MS.gene034160.t1 MTR_5g091130 31.105 389 236 15 69 432 173 554 6.20e-36 139
MS.gene034160.t1 MTR_3g068000 29.365 378 219 16 90 431 76 441 6.58e-31 124
MS.gene034160.t1 MTR_8g103880 29.737 380 232 16 67 431 96 455 9.56e-31 123
MS.gene034160.t1 MTR_2g070430 28.571 462 275 17 6 428 5 450 7.57e-29 118
MS.gene034160.t1 MTR_7g105850 30.044 456 234 24 41 433 34 467 4.65e-28 115
MS.gene034160.t1 MTR_7g080230 26.773 437 269 19 31 432 11 431 8.36e-28 115
MS.gene034160.t1 MTR_3g065160 28.075 374 221 16 88 433 75 428 3.91e-27 112
MS.gene034160.t1 MTR_2g055840 26.984 378 242 13 79 431 5 373 9.66e-26 108
MS.gene034160.t1 MTR_3g053980 29.315 365 235 13 78 431 133 485 3.01e-24 105
MS.gene034160.t1 MTR_0945s0010 40.000 125 71 4 310 432 1 123 3.42e-24 98.6
MS.gene034160.t1 MTR_7g062360 26.984 378 226 15 90 431 62 425 1.93e-23 102
MS.gene034160.t1 MTR_7g021510 26.963 382 226 18 86 432 71 434 1.98e-23 102
MS.gene034160.t1 MTR_8g094590 26.146 371 231 14 86 431 72 424 4.54e-23 101
MS.gene034160.t1 MTR_5g077850 26.260 377 228 15 88 433 82 439 6.08e-23 100
MS.gene034160.t1 MTR_1g078240 26.108 406 251 20 62 432 39 430 7.11e-22 98.2
MS.gene034160.t1 MTR_1g029810 26.330 376 229 16 86 433 56 411 1.17e-21 97.1
MS.gene034160.t1 MTR_1g029810 26.330 376 229 16 86 433 56 411 1.33e-21 97.1
MS.gene034160.t1 MTR_3g096930 25.000 388 237 16 79 433 70 436 1.86e-21 96.7
MS.gene034160.t1 MTR_2g103340 42.735 117 23 1 21 137 33 105 2.19e-21 91.7
MS.gene034160.t1 MTR_1g078240 26.716 408 247 21 62 432 39 431 3.82e-21 95.9
MS.gene034160.t1 MTR_6g084710 26.649 379 233 15 86 434 190 553 4.47e-21 96.3
MS.gene034160.t1 MTR_4g095270 25.424 413 263 17 42 433 37 425 9.25e-21 95.1
MS.gene034160.t1 MTR_5g016260 25.405 370 231 16 86 433 87 433 1.02e-20 95.1
MS.gene034160.t1 MTR_4g095270 25.424 413 263 17 42 433 37 425 1.09e-20 95.1
MS.gene034160.t1 MTR_5g016260 25.405 370 231 16 86 433 87 433 1.29e-20 94.7
MS.gene034160.t1 MTR_4g023650 25.441 397 249 16 67 433 42 421 1.63e-20 93.6
MS.gene034160.t1 MTR_8g094590 28.777 278 171 10 86 354 72 331 3.37e-20 92.4
MS.gene034160.t1 MTR_4g100990 27.603 413 215 20 87 431 74 470 5.22e-20 92.4
MS.gene034160.t1 MTR_1g037630 28.235 425 264 17 27 432 27 429 5.94e-20 92.0
MS.gene034160.t1 MTR_4g056420 27.394 376 230 17 86 432 76 437 8.34e-20 92.0
MS.gene034160.t1 MTR_8g094590 29.297 256 155 9 86 333 72 309 1.27e-19 89.4
MS.gene034160.t1 MTR_5g012490 26.536 407 226 19 87 431 74 469 2.51e-19 90.5
MS.gene034160.t1 MTR_7g080230 27.479 353 209 17 111 432 25 361 2.52e-19 89.7
MS.gene034160.t1 MTR_2g084550 28.912 377 224 18 86 432 76 438 5.54e-19 89.4
MS.gene034160.t1 MTR_3g014290 29.050 358 220 15 88 432 81 417 6.57e-19 88.6
MS.gene034160.t1 MTR_4g023630 25.441 397 249 16 67 433 42 421 7.54e-19 88.6
MS.gene034160.t1 MTR_1g078240 27.647 340 204 18 62 373 39 364 1.42e-18 87.4
MS.gene034160.t1 MTR_5g077920 25.459 381 228 14 88 433 69 428 1.45e-18 87.8
MS.gene034160.t1 MTR_6g084710 26.140 329 210 13 86 396 190 503 3.02e-18 87.4
MS.gene034160.t1 MTR_8g039540 28.795 448 262 24 16 432 13 434 4.96e-17 83.2
MS.gene034160.t1 MTR_5g080330 26.816 358 195 17 104 431 2 322 8.29e-17 82.4
MS.gene034160.t1 MTR_1g061940 25.936 374 240 18 86 432 82 445 3.17e-16 80.9
MS.gene034160.t1 MTR_7g102750 25.269 372 243 16 86 432 80 441 1.18e-15 79.3
MS.gene034160.t1 MTR_2g063110 23.270 477 279 20 29 431 45 508 2.58e-15 78.2
MS.gene034160.t1 MTR_3g100500 27.293 447 263 20 7 418 5 424 4.86e-14 73.9
MS.gene034160.t1 MTR_7g102750 26.623 308 196 14 86 373 80 377 7.92e-14 73.6
MS.gene034160.t1 MTR_8g075090 26.087 368 221 20 93 433 118 461 3.83e-13 71.6
MS.gene034160.t1 MTR_3g087780 23.632 457 262 22 29 431 27 450 8.71e-13 70.5
MS.gene034160.t1 MTR_8g075090 25.956 366 220 20 93 431 118 459 1.67e-12 69.3
MS.gene034160.t1 MTR_3g006820 22.506 391 259 15 72 433 39 414 1.54e-11 66.2
MS.gene034160.t1 MTR_5g061690 26.621 293 179 15 141 419 24 294 1.69e-11 65.9
MS.gene034160.t1 MTR_8g075090 25.836 329 202 17 93 398 118 427 8.75e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034160.t1 AT5G33340 48.418 411 203 8 28 433 30 436 8.20e-122 361
MS.gene034160.t1 AT1G64830 45.499 411 216 7 27 433 24 430 3.84e-121 359
MS.gene034160.t1 AT2G35615 44.444 441 217 12 16 432 8 444 5.55e-109 329
MS.gene034160.t1 AT1G31450 41.295 448 241 10 1 432 1 442 1.60e-97 300
MS.gene034160.t1 AT2G28010 39.450 436 207 16 6 435 11 395 2.50e-84 264
MS.gene034160.t1 AT2G28040 38.799 433 203 16 9 432 12 391 1.54e-79 251
MS.gene034160.t1 AT2G28040 39.518 415 191 15 27 432 19 382 1.72e-77 246
MS.gene034160.t1 AT2G28030 43.466 352 170 13 88 433 61 389 6.45e-76 242
MS.gene034160.t1 AT2G28220 37.011 435 221 15 7 432 8 398 3.90e-74 238
MS.gene034160.t1 AT2G28225 35.780 436 227 15 7 433 8 399 5.29e-67 219
MS.gene034160.t1 AT2G03200 35.308 439 239 16 23 433 39 460 1.89e-59 201
MS.gene034160.t1 AT1G01300 36.415 357 204 13 86 431 140 484 1.53e-52 183
MS.gene034160.t1 AT3G61820 35.813 363 204 13 86 431 133 483 3.27e-47 169
MS.gene034160.t1 AT3G18490 30.649 447 262 17 7 431 80 500 3.76e-47 169
MS.gene034160.t1 AT3G20015 30.365 438 260 16 21 431 51 470 1.54e-46 167
MS.gene034160.t1 AT1G25510 32.955 352 216 10 86 431 146 483 1.69e-43 159
MS.gene034160.t1 AT2G23945 27.409 467 288 15 1 433 1 450 1.21e-41 153
MS.gene034160.t1 AT4G30030 32.143 364 221 15 80 431 70 419 2.22e-41 152
MS.gene034160.t1 AT5G10770 35.733 375 206 16 78 432 115 474 1.87e-40 150
MS.gene034160.t1 AT5G10760 32.220 419 249 16 30 431 64 464 2.51e-40 150
MS.gene034160.t1 AT1G79720 34.501 371 193 19 88 431 135 482 1.92e-37 142
MS.gene034160.t1 AT3G12700 28.315 445 275 17 15 432 34 461 2.87e-36 139
MS.gene034160.t1 AT4G30040 30.425 447 257 24 5 426 10 427 3.20e-35 135
MS.gene034160.t1 AT3G25700 30.159 378 228 16 86 434 82 452 5.54e-35 135
MS.gene034160.t1 AT2G42980 30.628 382 240 13 73 431 145 524 6.41e-34 133
MS.gene034160.t1 AT3G59080 29.737 380 244 14 73 431 155 532 2.31e-32 129
MS.gene034160.t1 AT2G39710 29.947 374 210 17 90 426 67 425 3.03e-32 127
MS.gene034160.t1 AT5G36260 28.460 383 214 17 86 432 76 434 4.23e-31 125
MS.gene034160.t1 AT5G02190 27.151 372 223 16 97 431 82 442 3.29e-28 116
MS.gene034160.t1 AT1G66180 25.397 378 229 15 88 433 72 428 3.10e-25 107
MS.gene034160.t1 AT3G59080 27.467 375 223 12 73 431 155 496 4.79e-25 107
MS.gene034160.t1 AT1G09750 27.820 399 246 15 58 431 67 448 5.03e-25 107
MS.gene034160.t1 AT5G37540 27.200 375 226 16 88 431 80 438 2.57e-24 105
MS.gene034160.t1 AT3G54400 28.974 390 237 17 63 432 56 425 4.95e-24 103
MS.gene034160.t1 AT3G02740 26.902 368 238 16 86 432 83 440 8.55e-24 103
MS.gene034160.t1 AT5G45120 27.119 413 215 19 88 431 83 478 1.25e-23 103
MS.gene034160.t1 AT3G02740 27.222 360 231 16 94 432 16 365 1.32e-23 102
MS.gene034160.t1 AT1G65240 25.000 420 258 17 50 432 28 427 4.72e-23 101
MS.gene034160.t1 AT4G33490 26.185 401 238 18 69 433 35 413 1.72e-22 99.4
MS.gene034160.t1 AT5G43100 27.248 367 229 14 84 432 72 418 2.42e-22 99.4
MS.gene034160.t1 AT1G44130 27.068 399 236 18 69 433 24 401 2.68e-22 98.6
MS.gene034160.t1 AT5G43100 27.248 367 229 14 84 432 72 418 5.71e-22 99.0
MS.gene034160.t1 AT1G49050 24.157 356 238 12 105 434 49 398 2.36e-20 93.2
MS.gene034160.t1 AT5G22850 27.778 378 227 18 86 432 79 441 6.76e-20 92.4
MS.gene034160.t1 AT2G17760 27.198 364 217 18 93 431 109 449 7.42e-20 92.4
MS.gene034160.t1 AT1G49050 24.157 356 238 12 105 434 222 571 7.73e-20 92.4
MS.gene034160.t1 AT4G33490 27.703 296 174 13 69 344 32 307 9.24e-19 88.2
MS.gene034160.t1 AT1G05840 25.526 380 232 15 86 432 78 439 1.56e-18 88.2
MS.gene034160.t1 AT5G07030 28.153 444 265 21 7 419 22 442 6.03e-18 86.3
MS.gene034160.t1 AT3G50050 25.341 367 236 14 86 433 91 438 7.11e-18 86.7
MS.gene034160.t1 AT2G36670 26.216 370 241 17 86 432 98 458 3.59e-17 84.0
MS.gene034160.t1 AT1G08210 26.203 374 235 17 86 432 130 489 5.11e-17 83.6
MS.gene034160.t1 AT1G08210 26.203 374 235 17 86 432 116 475 5.13e-17 83.6
MS.gene034160.t1 AT2G36670 26.519 362 234 17 94 432 40 392 5.42e-17 83.2
MS.gene034160.t1 AT2G36670 26.519 362 234 17 94 432 40 392 5.42e-17 83.2
MS.gene034160.t1 AT1G08210 26.203 374 235 17 86 432 82 441 5.85e-17 83.2
MS.gene034160.t1 AT2G36670 26.519 362 234 17 94 432 111 463 5.87e-17 83.6
MS.gene034160.t1 AT1G49050 26.800 250 161 9 105 342 222 461 4.71e-16 80.5
MS.gene034160.t1 AT3G42550 22.642 371 223 12 78 432 68 390 5.78e-16 80.1
MS.gene034160.t1 AT1G08210 27.885 312 185 15 86 373 82 377 3.57e-14 74.7
MS.gene034160.t1 AT5G24820 26.122 245 161 8 200 435 170 403 1.72e-12 69.3
MS.gene034160.t1 AT3G51340 25.414 362 225 18 93 431 108 447 2.14e-12 69.3
MS.gene034160.t1 AT3G25700 30.709 127 79 4 86 204 82 207 4.33e-12 67.8
MS.gene034160.t1 AT3G51360 24.377 361 232 13 93 431 10 351 1.36e-11 66.2

Find 83 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAATGTGCAGTGATTA+TGG 0.131217 4.3:+87443979 None:intergenic
ATCTGGTATTTATCATAATT+TGG 0.150549 4.3:-87444104 MS.gene034160:CDS
GGAGAAGCTGCTATAGTTTC+TGG 0.180325 4.3:-87444298 MS.gene034160:CDS
TCAACGAGCAAACTCAATTT+TGG 0.205188 4.3:-87444319 MS.gene034160:CDS
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.3:-87444358 MS.gene034160:CDS
AGCGGGCATCACAGTGGATT+TGG 0.236368 4.3:+87444840 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.251212 4.3:-87444784 MS.gene034160:CDS
CCTAAAACCGTGATAGGTTT+TGG 0.252734 4.3:-87444481 MS.gene034160:CDS
CTACTCAATAAAGGCATCTT+TGG 0.257937 4.3:-87443875 MS.gene034160:CDS
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.3:-87444359 MS.gene034160:CDS
ACAATACCATATAACTTAAA+TGG 0.274831 4.3:+87444778 None:intergenic
TCATTTCCTAAAACCGTGAT+AGG 0.315869 4.3:-87444487 MS.gene034160:CDS
CGTGTCTCTAATAAATCAAT+TGG 0.324779 4.3:-87444395 MS.gene034160:CDS
AATCAATTGGGTTCATCAAT+TGG 0.334833 4.3:-87444382 MS.gene034160:CDS
TGGATTTGGGTGTGTTAGTA+AGG 0.346495 4.3:+87444854 None:intergenic
AACAAAGTCTACACTCAATA+TGG 0.354389 4.3:+87443770 None:intergenic
GGTAACATCATAGTTGATTC+AGG 0.362416 4.3:-87444148 MS.gene034160:CDS
GCGGGCATCACAGTGGATTT+GGG 0.367396 4.3:+87444841 None:intergenic
GTCCGATCTCTACTCAATAA+AGG 0.384414 4.3:-87443884 MS.gene034160:CDS
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.3:-87444421 MS.gene034160:CDS
ACCTTTGCTCGAGTTTCTGA+TGG 0.391081 4.3:-87443926 MS.gene034160:CDS
TGCGAATATAGTATTAAGTA+TGG 0.394209 4.3:-87444583 MS.gene034160:CDS
TCAAAATGTGCAGTGATTAT+GGG 0.395459 4.3:+87443980 None:intergenic
CAATCAAATGGTAGTGTTGA+TGG 0.396941 4.3:-87444169 MS.gene034160:CDS
AGCTCTGGTGTGGTTGGCCT+TGG 0.398793 4.3:-87444427 MS.gene034160:CDS
AACAATACATTGTCATTTGA+AGG 0.406579 4.3:-87444454 MS.gene034160:CDS
TACTCAATAAAGGCATCTTT+GGG 0.415339 4.3:-87443874 MS.gene034160:CDS
ATCACAAGGAGATCTTAGTT+TGG 0.415779 4.3:-87444548 MS.gene034160:CDS
ATCATATTTATTTGATGCAA+CGG 0.425117 4.3:+87444006 None:intergenic
TCTCTTATTGTTTGGGGCCA+TGG 0.430440 4.3:-87444351 MS.gene034160:CDS
GATTAAAGATAGGAGTCGTT+TGG 0.435507 4.3:+87444698 None:intergenic
CATAAGAAACTCACCTTTAG+CGG 0.438405 4.3:+87444822 None:intergenic
TTATATGGTATTGTTGATAC+AGG 0.443331 4.3:-87444769 MS.gene034160:CDS
ATACAGGTAGTGACATGGTT+TGG 0.456250 4.3:-87444753 MS.gene034160:CDS
CCTTTGCTCGAGTTTCTGAT+GGG 0.459517 4.3:-87443925 MS.gene034160:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.3:-87444520 MS.gene034160:CDS
TCATTTGAAGGCAAGAGCTC+TGG 0.470790 4.3:-87444442 MS.gene034160:CDS
TGGTGGGGTACCGACTGAAT+AGG 0.480047 4.3:+87444798 None:intergenic
GGCAAGAGCTCTGGTGTGGT+TGG 0.491732 4.3:-87444433 MS.gene034160:CDS
TACCATATAACTTAAATGGT+GGG 0.492301 4.3:+87444782 None:intergenic
CTTTCTAATGCTCTAAGCAA+TGG 0.494056 4.3:-87445009 MS.gene034160:CDS
GAATTCAAAGGATTAAAGAT+AGG 0.495849 4.3:+87444688 None:intergenic
TCCACTGTGATGCCCGCTAA+AGG 0.499859 4.3:-87444835 MS.gene034160:CDS
AGTCTACACTCAATATGGTT+TGG 0.504081 4.3:+87443775 None:intergenic
TTGTAACTTGATGAATTCAA+AGG 0.507205 4.3:+87444676 None:intergenic
AAGCGTGCAAAGGATCCAAC+TGG 0.524991 4.3:-87444052 MS.gene034160:CDS
GTGTCTCTAATAAATCAATT+GGG 0.531292 4.3:-87444394 MS.gene034160:CDS
TATAGTATTAAGTATGGTGA+TGG 0.532681 4.3:-87444577 MS.gene034160:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.3:-87444217 MS.gene034160:CDS
GCTGCTATAGTTTCTGGTCA+TGG 0.541311 4.3:-87444292 MS.gene034160:CDS
TGCCTTTATTGAGTAGAGAT+CGG 0.542952 4.3:+87443882 None:intergenic
GCAGTGACAGTTGTACCATC+TGG 0.543688 4.3:-87444121 MS.gene034160:CDS
TCATAATTTGGTATCAGCTG+TGG 0.549938 4.3:-87444092 MS.gene034160:CDS
GCTCAACAGAATTTGTTGGT+TGG 0.552408 4.3:-87443845 MS.gene034160:CDS
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.3:-87444357 MS.gene034160:CDS
CAATTGATTTATTAGAGACA+CGG 0.558463 4.3:+87444396 None:intergenic
GTTATGTCCAAAACCTATCA+CGG 0.558819 4.3:+87444474 None:intergenic
TATGGTTTGGTACAATCAGT+AGG 0.559170 4.3:+87443788 None:intergenic
ATACCATATAACTTAAATGG+TGG 0.565852 4.3:+87444781 None:intergenic
CTCGTTGAGTTAGACGACCA+TGG 0.566118 4.3:+87444334 None:intergenic
GAGACACGGGTCCATTTCCA+AGG 0.570796 4.3:+87444410 None:intergenic
AGATTAAGAAAACCACCAGT+TGG 0.573212 4.3:+87444037 None:intergenic
GGATTTGGGTGTGTTAGTAA+GGG 0.573804 4.3:+87444855 None:intergenic
ACCATATAACTTAAATGGTG+GGG 0.576438 4.3:+87444783 None:intergenic
ATAAGAAACTCACCTTTAGC+GGG 0.577860 4.3:+87444823 None:intergenic
CCCATCAGAAACTCGAGCAA+AGG 0.593408 4.3:+87443925 None:intergenic
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.3:-87444379 MS.gene034160:CDS
AATTGATTTATTAGAGACAC+GGG 0.597238 4.3:+87444397 None:intergenic
CATCATAGTTGATTCAGGTA+CGG 0.597806 4.3:-87444143 MS.gene034160:CDS
CTTTGCTCGAGTTTCTGATG+GGG 0.607379 4.3:-87443924 MS.gene034160:CDS
GTAATGATCGACATGATCGA+TGG 0.608118 4.3:+87444888 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.3:+87444507 None:intergenic
AGTTAAACTGAAGCGTGCAA+AGG 0.614291 4.3:-87444062 MS.gene034160:CDS
TTTCTTATGACCTATTCAGT+CGG 0.625475 4.3:-87444808 MS.gene034160:CDS
TGTTGATACAGGTAGTGACA+TGG 0.630223 4.3:-87444758 MS.gene034160:CDS
TGGTTGTAACAATGTTCACA+AGG 0.636110 4.3:+87444718 None:intergenic
AAATTATGATAAATACCAGA+TGG 0.652242 4.3:+87444106 None:intergenic
TGAAGGCAAGAGCTCTGGTG+TGG 0.663721 4.3:-87444437 MS.gene034160:CDS
ACCTTTAGCGGGCATCACAG+TGG 0.667290 4.3:+87444834 None:intergenic
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.3:+87444504 None:intergenic
CGTGCAAAGGATCCAACTGG+TGG 0.682034 4.3:-87444049 MS.gene034160:CDS
GGTGATGGATCAATATCACA+AGG 0.695175 4.3:-87444562 MS.gene034160:CDS
GCATAGCATTGACAACACGT+TGG 0.726271 4.3:+87444917 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAGTAAGCAATTTTT+GGG + chr4.3:87444617-87444636 None:intergenic 15.0%
!! AAATTATGATAAATACCAGA+TGG + chr4.3:87444750-87444769 None:intergenic 20.0%
!! ACAATACCATATAACTTAAA+TGG + chr4.3:87444078-87444097 None:intergenic 20.0%
!! ATCATATTTATTTGATGCAA+CGG + chr4.3:87444850-87444869 None:intergenic 20.0%
!! ATCTGGTATTTATCATAATT+TGG - chr4.3:87444749-87444768 MS.gene034160:CDS 20.0%
!!! AGTTTTGTTTTTATTACAAG+AGG + chr4.3:87444250-87444269 None:intergenic 20.0%
!!! GTTATTAAGTAAGCAATTTT+TGG + chr4.3:87444618-87444637 None:intergenic 20.0%
!!! TTACATTTTTTAGACAAACA+AGG + chr4.3:87444213-87444232 None:intergenic 20.0%
! ATACCATATAACTTAAATGG+TGG + chr4.3:87444075-87444094 None:intergenic 25.0%
! GAATTCAAAGGATTAAAGAT+AGG + chr4.3:87444168-87444187 None:intergenic 25.0%
! GTGTCTCTAATAAATCAATT+GGG - chr4.3:87444459-87444478 MS.gene034160:CDS 25.0%
! TACCATATAACTTAAATGGT+GGG + chr4.3:87444074-87444093 None:intergenic 25.0%
! TATAGTATTAAGTATGGTGA+TGG - chr4.3:87444276-87444295 MS.gene034160:CDS 25.0%
! TGCGAATATAGTATTAAGTA+TGG - chr4.3:87444270-87444289 MS.gene034160:CDS 25.0%
! TTATATGGTATTGTTGATAC+AGG - chr4.3:87444084-87444103 MS.gene034160:CDS 25.0%
! TTGTAACTTGATGAATTCAA+AGG + chr4.3:87444180-87444199 None:intergenic 25.0%
!! AACAATACATTGTCATTTGA+AGG - chr4.3:87444399-87444418 MS.gene034160:CDS 25.0%
!! AATTGATTTATTAGAGACAC+GGG + chr4.3:87444459-87444478 None:intergenic 25.0%
!! CAATTGATTTATTAGAGACA+CGG + chr4.3:87444460-87444479 None:intergenic 25.0%
!! GAATTTTCAAAGCAATCAAA+TGG - chr4.3:87444672-87444691 MS.gene034160:CDS 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.3:87444352-87444371 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG - chr4.3:87444471-87444490 MS.gene034160:CDS 30.0%
ACCATATAACTTAAATGGTG+GGG + chr4.3:87444073-87444092 None:intergenic 30.0%
CGTGTCTCTAATAAATCAAT+TGG - chr4.3:87444458-87444477 MS.gene034160:CDS 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.3:87444496-87444515 MS.gene034160:CDS 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.3:87444495-87444514 MS.gene034160:CDS 30.0%
TCAAAATGTGCAGTGATTAT+GGG + chr4.3:87444876-87444895 None:intergenic 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.3:87444494-87444513 MS.gene034160:CDS 30.0%
TTCAAAATGTGCAGTGATTA+TGG + chr4.3:87444877-87444896 None:intergenic 30.0%
TTTCTTATGACCTATTCAGT+CGG - chr4.3:87444045-87444064 MS.gene034160:CDS 30.0%
! ATAATCACTGCACATTTTGA+AGG - chr4.3:87444876-87444895 MS.gene034160:CDS 30.0%
! TTTTGCTCAACAGAATTTGT+TGG - chr4.3:87445004-87445023 MS.gene034160:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.3:87444636-87444655 MS.gene034160:CDS 30.0%
!! TACTCAATAAAGGCATCTTT+GGG - chr4.3:87444979-87444998 MS.gene034160:CDS 30.0%
!!! TTTGGGTCTTTTTTCACTAA+AGG + chr4.3:87444600-87444619 None:intergenic 30.0%
AGATTAAGAAAACCACCAGT+TGG + chr4.3:87444819-87444838 None:intergenic 35.0%
ATAAGAAACTCACCTTTAGC+GGG + chr4.3:87444033-87444052 None:intergenic 35.0%
ATCACAAGGAGATCTTAGTT+TGG - chr4.3:87444305-87444324 MS.gene034160:CDS 35.0%
CATAAGAAACTCACCTTTAG+CGG + chr4.3:87444034-87444053 None:intergenic 35.0%
GATTAAAGATAGGAGTCGTT+TGG + chr4.3:87444158-87444177 None:intergenic 35.0%
GTTATGTCCAAAACCTATCA+CGG + chr4.3:87444382-87444401 None:intergenic 35.0%
TCATAATTTGGTATCAGCTG+TGG - chr4.3:87444761-87444780 MS.gene034160:CDS 35.0%
TCATTTCCTAAAACCGTGAT+AGG - chr4.3:87444366-87444385 MS.gene034160:CDS 35.0%
TGCCTTTATTGAGTAGAGAT+CGG + chr4.3:87444974-87444993 None:intergenic 35.0%
TGGTTGTAACAATGTTCACA+AGG + chr4.3:87444138-87444157 None:intergenic 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr4.3:87444069-87444088 MS.gene034160:CDS 35.0%
! CAATCAAATGGTAGTGTTGA+TGG - chr4.3:87444684-87444703 MS.gene034160:CDS 35.0%
! CATCATAGTTGATTCAGGTA+CGG - chr4.3:87444710-87444729 MS.gene034160:CDS 35.0%
! CTTTCTAATGCTCTAAGCAA+TGG - chr4.3:87443844-87443863 MS.gene034160:CDS 35.0%
! GGTAACATCATAGTTGATTC+AGG - chr4.3:87444705-87444724 MS.gene034160:CDS 35.0%
! TCAACGAGCAAACTCAATTT+TGG - chr4.3:87444534-87444553 MS.gene034160:CDS 35.0%
!! CTACTCAATAAAGGCATCTT+TGG - chr4.3:87444978-87444997 MS.gene034160:CDS 35.0%
!! TATGGTTTGGTACAATCAGT+AGG + chr4.3:87445068-87445087 None:intergenic 35.0%
!!! CGTTGGTATTTGTTTTGTGT+AGG + chr4.3:87443922-87443941 None:intergenic 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG + chr4.3:87443918-87443937 None:intergenic 35.0%
AGTTAAACTGAAGCGTGCAA+AGG - chr4.3:87444791-87444810 MS.gene034160:CDS 40.0%
ATACAGGTAGTGACATGGTT+TGG - chr4.3:87444100-87444119 MS.gene034160:CDS 40.0%
CAATTGGGTTCATCAATTGG+TGG - chr4.3:87444474-87444493 MS.gene034160:CDS 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.3:87444333-87444352 MS.gene034160:CDS 40.0%
GGATTTGGGTGTGTTAGTAA+GGG + chr4.3:87444001-87444020 None:intergenic 40.0%
GTAATGATCGACATGATCGA+TGG + chr4.3:87443968-87443987 None:intergenic 40.0%
GTCCGATCTCTACTCAATAA+AGG - chr4.3:87444969-87444988 MS.gene034160:CDS 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.3:87444349-87444368 None:intergenic 40.0%
TGGATTTGGGTGTGTTAGTA+AGG + chr4.3:87444002-87444021 None:intergenic 40.0%
TGTTGATACAGGTAGTGACA+TGG - chr4.3:87444095-87444114 MS.gene034160:CDS 40.0%
! CCAAAACCTATCACGGTTTT+AGG + chr4.3:87444375-87444394 None:intergenic 40.0%
! CCTAAAACCGTGATAGGTTT+TGG - chr4.3:87444372-87444391 MS.gene034160:CDS 40.0%
!! GCTCAACAGAATTTGTTGGT+TGG - chr4.3:87445008-87445027 MS.gene034160:CDS 40.0%
!! GGTGATGGATCAATATCACA+AGG - chr4.3:87444291-87444310 MS.gene034160:CDS 40.0%
!!! TTTTGTGTAGGTTGGTAGAG+TGG + chr4.3:87443910-87443929 None:intergenic 40.0%
ACCTTTGCTCGAGTTTCTGA+TGG - chr4.3:87444927-87444946 MS.gene034160:CDS 45.0%
CCTTTGCTCGAGTTTCTGAT+GGG - chr4.3:87444928-87444947 MS.gene034160:CDS 45.0%
CTTTGCTCGAGTTTCTGATG+GGG - chr4.3:87444929-87444948 MS.gene034160:CDS 45.0%
GGAGAAGCTGCTATAGTTTC+TGG - chr4.3:87444555-87444574 MS.gene034160:CDS 45.0%
TCATTTGAAGGCAAGAGCTC+TGG - chr4.3:87444411-87444430 MS.gene034160:CDS 45.0%
! TCTCTTATTGTTTGGGGCCA+TGG - chr4.3:87444502-87444521 MS.gene034160:CDS 45.0%
!! GCATAGCATTGACAACACGT+TGG + chr4.3:87443939-87443958 None:intergenic 45.0%
!! GCTGCTATAGTTTCTGGTCA+TGG - chr4.3:87444561-87444580 MS.gene034160:CDS 45.0%
!! GTGGTGATTTTGAAGAGTCG+CGG + chr4.3:87443891-87443910 None:intergenic 45.0%
AAGCGTGCAAAGGATCCAAC+TGG - chr4.3:87444801-87444820 MS.gene034160:CDS 50.0%
CCCATCAGAAACTCGAGCAA+AGG + chr4.3:87444931-87444950 None:intergenic 50.0%
CTCGTTGAGTTAGACGACCA+TGG + chr4.3:87444522-87444541 None:intergenic 50.0%
GCAGTGACAGTTGTACCATC+TGG - chr4.3:87444732-87444751 MS.gene034160:CDS 50.0%
!! GTGATTTTGAAGAGTCGCGG+TGG + chr4.3:87443888-87443907 None:intergenic 50.0%
CGTGCAAAGGATCCAACTGG+TGG - chr4.3:87444804-87444823 MS.gene034160:CDS 55.0%
GAGACACGGGTCCATTTCCA+AGG + chr4.3:87444446-87444465 None:intergenic 55.0%
TCCACTGTGATGCCCGCTAA+AGG - chr4.3:87444018-87444037 MS.gene034160:CDS 55.0%
! ACCTTTAGCGGGCATCACAG+TGG + chr4.3:87444022-87444041 None:intergenic 55.0%
! AGCGGGCATCACAGTGGATT+TGG + chr4.3:87444016-87444035 None:intergenic 55.0%
! GCGGGCATCACAGTGGATTT+GGG + chr4.3:87444015-87444034 None:intergenic 55.0%
! TGAAGGCAAGAGCTCTGGTG+TGG - chr4.3:87444416-87444435 MS.gene034160:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.3:87444432-87444451 MS.gene034160:CDS 55.0%
!! TGGTGGGGTACCGACTGAAT+AGG + chr4.3:87444058-87444077 None:intergenic 55.0%
! GGCAAGAGCTCTGGTGTGGT+TGG - chr4.3:87444420-87444439 MS.gene034160:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.3:87444426-87444445 MS.gene034160:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 87443784 87445091 87443784 ID=MS.gene034160
chr4.3 mRNA 87443784 87445091 87443784 ID=MS.gene034160.t1;Parent=MS.gene034160
chr4.3 exon 87443784 87445091 87443784 ID=MS.gene034160.t1.exon1;Parent=MS.gene034160.t1
chr4.3 CDS 87443784 87445091 87443784 ID=cds.MS.gene034160.t1;Parent=MS.gene034160.t1
Gene Sequence

>MS.gene034160

ATGAGTATGTGTTATTTTCTTGCTCTTCAGCTTTTTTCTTTTTCTTGTTTTATGATTTCTCTTTCTAATGCTCTAAGCAATGGCTTTAGTGTTGAACTCATCCACCGCGACTCTTCAAAATCACCACTCTACCAACCTACACAAAACAAATACCAACGTGTTGTCAATGCTATGCGTCGTTCCATCGATCATGTCGATCATTACTACAAACATTCCCTTACTAACACACCCAAATCCACTGTGATGCCCGCTAAAGGTGAGTTTCTTATGACCTATTCAGTCGGTACCCCACCATTTAAGTTATATGGTATTGTTGATACAGGTAGTGACATGGTTTGGCTTCAATCTAAGCCTTGTGAACATTGTTACAACCAAACGACTCCTATCTTTAATCCTTTGAATTCATCAAGTTACAAAAACAATATTCCTTGTTTGTCTAAAAAATGTAAGTCTTTTGATTCTACCTCTTGTAATAAAAACAAAACTTGCGAATATAGTATTAAGTATGGTGATGGATCAATATCACAAGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACCGTGATAGGTTTTGGACATAACAATACATTGTCATTTGAAGGCAAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCCGTGTCTCTAATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCATGGTCGTCTAACTCAACGAGCAAACTCAATTTTGGAGAAGCTGCTATAGTTTCTGGTCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCCAAAAATTGCTTACTTAATAACATTGAAAGCATTTAGTGTTGGAAACAAAAGAGTAGAATTTTCAAAGCAATCAAATGGTAGTGTTGATGGTAACATCATAGTTGATTCAGGTACGGCAGTGACAGTTGTACCATCTGGTATTTATCATAATTTGGTATCAGCTGTGGTAAAATCAGTTAAACTGAAGCGTGCAAAGGATCCAACTGGTGGTTTTCTTAATCTTTGTTATTCCGTTGCATCAAATAAATATGATTTTCCCATAATCACTGCACATTTTGAAGGTGCAGATGTTAAGTTGCATTCTATGAGCACCTTTGCTCGAGTTTCTGATGGGGTTGTTTGTTTGACTCTTGTCCGATCTCTACTCAATAAAGGCATCTTTGGGAATTTTGCTCAACAGAATTTGTTGGTTGGTTATGATCTTCAACAAAAAATTGTGTCTTTTAAGCCTACTGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene034160.t1

MSMCYFLALQLFSFSCFMISLSNALSNGFSVELIHRDSSKSPLYQPTQNKYQRVVNAMRRSIDHVDHYYKHSLTNTPKSTVMPAKGEFLMTYSVGTPPFKLYGIVDTGSDMVWLQSKPCEHCYNQTTPIFNPLNSSSYKNNIPCLSKKCKSFDSTSCNKNKTCEYSIKYGDGSISQGDLSLETLTLDSTSGSSISFPKTVIGFGHNNTLSFEGKSSGVVGLGNGPVSLINQLGSSIGGKFSYCLGPWSSNSTSKLNFGEAAIVSGHGVVSTPLVKKDPKIAYLITLKAFSVGNKRVEFSKQSNGSVDGNIIVDSGTAVTVVPSGIYHNLVSAVVKSVKLKRAKDPTGGFLNLCYSVASNKYDFPIITAHFEGADVKLHSMSTFARVSDGVVCLTLVRSLLNKGIFGNFAQQNLLVGYDLQQKIVSFKPTDCTKPY