AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034173

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034173.t1 MTR_8g096990 68.883 376 113 3 2 375 58 431 0.0 520
MS.gene034173.t1 MTR_5g080960 68.254 378 114 4 2 375 58 433 0.0 509
MS.gene034173.t1 MTR_5g080890 67.725 378 116 4 2 375 58 433 1.82e-178 503
MS.gene034173.t1 MTR_5g044770 67.819 376 116 4 2 375 58 430 7.26e-178 501
MS.gene034173.t1 MTR_8g038280 65.526 380 123 3 2 375 58 435 1.15e-176 498
MS.gene034173.t1 MTR_8g038250 65.969 382 98 5 2 375 58 415 2.46e-172 487
MS.gene034173.t1 MTR_8g038310 57.825 377 140 3 1 377 58 415 1.94e-157 449
MS.gene034173.t1 MTR_6g090290 60.209 382 143 4 1 375 57 436 1.30e-149 430
MS.gene034173.t1 MTR_4g109760 61.273 377 117 4 2 375 48 398 1.12e-146 421
MS.gene034173.t1 MTR_3g028120 53.525 383 166 7 3 375 58 438 7.86e-135 392
MS.gene034173.t1 MTR_2g067830 54.167 384 164 9 1 375 26 406 1.05e-126 370
MS.gene034173.t1 MTR_5g038360 49.482 386 180 10 1 375 55 436 1.76e-117 348
MS.gene034173.t1 MTR_0024s0040 50.515 388 174 9 1 375 28 410 2.68e-117 347
MS.gene034173.t1 MTR_6g088760 53.143 350 150 8 3 341 59 405 4.71e-114 342
MS.gene034173.t1 MTR_2g105300 50.394 381 155 10 1 372 26 381 2.27e-112 333
MS.gene034173.t1 MTR_5g044570 44.819 386 172 11 1 375 56 411 4.35e-99 300
MS.gene034173.t1 MTR_5g008070 42.440 377 205 8 6 374 53 425 4.76e-92 283
MS.gene034173.t1 MTR_2g059490 44.915 354 184 9 30 374 58 409 1.14e-90 280
MS.gene034173.t1 MTR_2g059460 42.368 380 199 12 7 375 36 406 4.44e-89 274
MS.gene034173.t1 MTR_0036s0070 39.842 379 210 9 3 375 60 426 1.01e-88 274
MS.gene034173.t1 MTR_0036s0050 40.625 384 209 9 3 375 60 435 1.98e-88 274
MS.gene034173.t1 MTR_0036s0110 40.164 366 200 8 21 375 85 442 2.03e-85 266
MS.gene034173.t1 MTR_5g008150 41.737 357 192 10 29 375 62 412 6.10e-83 259
MS.gene034173.t1 MTR_5g008110 40.896 357 194 10 29 375 66 415 5.67e-82 256
MS.gene034173.t1 MTR_1g060750 36.340 377 208 9 5 375 26 376 1.32e-69 223
MS.gene034173.t1 MTR_3g097150 36.695 357 209 9 28 375 100 448 3.58e-66 216
MS.gene034173.t1 MTR_4g045857 44.130 247 130 6 15 254 43 288 7.61e-57 187
MS.gene034173.t1 MTR_5g008090 37.667 300 172 9 86 375 20 314 3.15e-55 184
MS.gene034173.t1 MTR_1g039270 32.768 354 220 10 28 373 126 469 1.11e-50 176
MS.gene034173.t1 MTR_6g088765 42.259 239 106 5 105 342 3 210 3.91e-49 166
MS.gene034173.t1 MTR_1g115210 32.861 353 220 10 28 373 144 486 2.51e-47 168
MS.gene034173.t1 MTR_4g008110 73.684 114 9 1 103 216 45 137 6.42e-46 154
MS.gene034173.t1 MTR_7g117430 32.584 356 220 12 28 373 139 484 2.14e-45 162
MS.gene034173.t1 MTR_1g111350 30.312 353 225 10 28 373 129 467 1.32e-41 152
MS.gene034173.t1 MTR_3g046300 32.552 384 237 11 12 375 79 460 6.48e-41 150
MS.gene034173.t1 MTR_6g085010 31.034 348 224 9 30 373 156 491 2.80e-39 146
MS.gene034173.t1 MTR_3g053710 31.967 366 224 13 23 373 111 466 4.50e-38 142
MS.gene034173.t1 MTR_5g091130 29.948 384 246 11 12 374 173 554 3.90e-36 138
MS.gene034173.t1 MTR_2g028190 31.707 369 226 14 21 373 93 451 9.52e-36 136
MS.gene034173.t1 MTR_4g007740 29.971 347 209 11 47 374 147 478 7.77e-35 134
MS.gene034173.t1 MTR_8g103880 29.121 364 230 14 10 361 96 443 3.42e-33 129
MS.gene034173.t1 MTR_3g068000 28.424 387 220 15 28 373 71 441 2.58e-29 118
MS.gene034173.t1 MTR_2g055840 26.525 377 244 12 22 373 5 373 1.06e-27 112
MS.gene034173.t1 MTR_3g053980 29.268 369 238 14 17 373 128 485 1.54e-26 110
MS.gene034173.t1 MTR_2g070430 29.155 367 217 13 37 370 94 450 5.02e-26 108
MS.gene034173.t1 MTR_7g105850 29.073 399 211 19 29 375 89 467 1.26e-23 102
MS.gene034173.t1 MTR_7g080230 26.178 382 239 17 23 374 63 431 3.70e-23 100
MS.gene034173.t1 MTR_3g065160 24.745 392 244 14 18 375 54 428 8.13e-23 99.8
MS.gene034173.t1 MTR_4g023650 26.650 394 235 17 16 375 48 421 7.07e-22 96.7
MS.gene034173.t1 MTR_0945s0010 37.903 124 74 3 253 374 1 123 1.70e-20 87.8
MS.gene034173.t1 MTR_3g014290 29.032 341 215 14 31 361 81 404 4.93e-20 91.3
MS.gene034173.t1 MTR_1g037630 29.032 341 215 14 31 361 93 416 6.88e-20 90.9
MS.gene034173.t1 MTR_1g029810 26.371 383 230 19 23 375 51 411 7.58e-20 90.9
MS.gene034173.t1 MTR_1g029810 26.110 383 231 18 23 375 51 411 8.27e-20 90.9
MS.gene034173.t1 MTR_5g016260 24.590 366 240 14 28 375 86 433 9.79e-20 91.3
MS.gene034173.t1 MTR_7g062360 26.263 396 241 16 15 373 44 425 1.80e-19 89.7
MS.gene034173.t1 MTR_7g021510 24.138 377 242 17 29 374 71 434 1.99e-19 90.1
MS.gene034173.t1 MTR_5g016260 25.269 372 230 16 28 375 86 433 1.99e-19 90.5
MS.gene034173.t1 MTR_4g056420 28.232 379 222 19 29 374 76 437 3.46e-19 89.4
MS.gene034173.t1 MTR_2g084550 28.647 377 224 18 29 374 76 438 5.60e-19 88.6
MS.gene034173.t1 MTR_4g095270 24.865 370 234 15 28 375 78 425 1.28e-18 87.8
MS.gene034173.t1 MTR_4g095270 24.865 370 234 15 28 375 78 425 1.72e-18 87.4
MS.gene034173.t1 MTR_8g094590 25.199 377 237 18 23 373 67 424 2.41e-18 86.7
MS.gene034173.t1 MTR_2g063110 24.570 407 230 18 29 373 117 508 2.46e-18 86.7
MS.gene034173.t1 MTR_1g078240 25.062 403 258 18 5 374 39 430 2.63e-18 86.7
MS.gene034173.t1 MTR_4g100990 26.942 412 220 19 30 374 74 471 4.03e-18 85.9
MS.gene034173.t1 MTR_4g023630 25.127 394 241 17 16 375 48 421 4.45e-18 85.5
MS.gene034173.t1 MTR_1g078240 25.926 405 253 19 5 374 39 431 5.22e-18 85.9
MS.gene034173.t1 MTR_8g039540 29.014 355 224 15 31 374 97 434 2.60e-17 83.6
MS.gene034173.t1 MTR_3g096930 24.147 381 239 16 23 375 78 436 1.03e-16 81.6
MS.gene034173.t1 MTR_5g077850 22.872 376 241 13 31 375 82 439 1.74e-16 80.9
MS.gene034173.t1 MTR_7g080230 25.429 350 219 16 54 374 25 361 4.15e-16 79.7
MS.gene034173.t1 MTR_1g078240 26.113 337 212 15 5 315 39 364 7.51e-16 78.6
MS.gene034173.t1 MTR_5g012490 24.878 410 230 20 30 374 74 470 8.65e-16 79.0
MS.gene034173.t1 MTR_8g094590 26.036 338 209 16 23 338 67 385 1.16e-15 78.2
MS.gene034173.t1 MTR_8g094590 27.519 258 160 10 23 272 67 305 2.66e-15 76.3
MS.gene034173.t1 MTR_3g100500 28.448 348 214 16 29 360 96 424 2.25e-13 71.6
MS.gene034173.t1 MTR_5g080330 25.698 358 198 17 47 373 2 322 9.39e-12 66.6
MS.gene034173.t1 MTR_5g061690 28.070 285 162 16 92 361 38 294 1.10e-11 66.2
MS.gene034173.t1 MTR_2g103340 71.795 39 11 0 42 80 67 105 1.69e-11 63.2
MS.gene034173.t1 MTR_3g087780 27.019 322 192 16 73 373 9 308 1.97e-11 65.5
MS.gene034173.t1 MTR_8g075090 25.613 367 223 19 36 375 118 461 2.29e-11 65.5
MS.gene034173.t1 MTR_3g087780 27.019 322 192 16 73 373 45 344 3.36e-11 64.7
MS.gene034173.t1 MTR_2g037710 27.700 213 127 10 169 373 12 205 4.73e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034173.t1 AT1G64830 45.953 383 196 6 1 375 51 430 8.73e-111 331
MS.gene034173.t1 AT5G33340 47.895 380 190 7 1 375 60 436 6.68e-108 323
MS.gene034173.t1 AT2G35615 43.734 391 198 10 3 374 57 444 3.24e-95 291
MS.gene034173.t1 AT1G31450 42.931 389 199 9 3 374 60 442 7.24e-88 273
MS.gene034173.t1 AT2G28010 42.308 364 184 10 19 377 53 395 2.63e-82 256
MS.gene034173.t1 AT2G28040 42.659 361 180 13 19 374 44 382 9.08e-79 247
MS.gene034173.t1 AT2G28040 42.308 364 183 13 19 377 53 394 9.48e-79 248
MS.gene034173.t1 AT2G28030 41.026 351 183 9 31 377 61 391 6.86e-76 240
MS.gene034173.t1 AT2G28220 41.408 355 180 9 31 377 67 401 4.13e-75 238
MS.gene034173.t1 AT2G28225 38.692 367 193 10 19 375 55 399 7.90e-69 222
MS.gene034173.t1 AT2G03200 36.508 378 211 12 17 375 93 460 1.05e-58 197
MS.gene034173.t1 AT1G01300 34.174 357 215 12 28 374 139 485 2.00e-48 171
MS.gene034173.t1 AT4G30030 34.890 364 206 15 25 373 72 419 1.52e-45 162
MS.gene034173.t1 AT3G18490 31.217 378 236 13 6 373 137 500 3.10e-45 162
MS.gene034173.t1 AT3G61820 32.597 362 218 12 28 373 132 483 2.42e-43 157
MS.gene034173.t1 AT1G25510 31.250 352 223 9 28 373 145 483 5.22e-43 156
MS.gene034173.t1 AT2G23945 29.428 367 225 13 31 375 96 450 3.75e-41 150
MS.gene034173.t1 AT3G20015 30.532 357 223 12 28 373 128 470 3.46e-40 148
MS.gene034173.t1 AT5G10770 33.508 382 222 16 9 374 109 474 1.38e-38 144
MS.gene034173.t1 AT5G10760 30.848 389 240 11 1 373 89 464 1.94e-38 143
MS.gene034173.t1 AT3G12700 31.302 361 230 12 29 374 104 461 9.23e-37 139
MS.gene034173.t1 AT2G42980 30.890 382 238 14 16 373 145 524 1.31e-36 139
MS.gene034173.t1 AT1G79720 33.516 364 205 16 31 373 135 482 1.68e-36 138
MS.gene034173.t1 AT3G25700 29.365 378 232 14 28 376 81 452 3.74e-35 134
MS.gene034173.t1 AT4G30040 30.851 376 211 18 19 368 75 427 9.17e-35 133
MS.gene034173.t1 AT3G59080 30.971 381 239 16 16 374 155 533 1.76e-34 134
MS.gene034173.t1 AT5G36260 28.342 374 225 18 29 374 76 434 4.08e-32 126
MS.gene034173.t1 AT2G39710 27.822 381 218 16 28 368 62 425 7.12e-28 114
MS.gene034173.t1 AT3G54400 28.691 359 220 16 31 374 88 425 8.63e-26 108
MS.gene034173.t1 AT3G59080 28.723 376 218 14 16 374 155 497 3.20e-25 107
MS.gene034173.t1 AT3G02740 27.100 369 235 16 29 374 83 440 4.10e-25 106
MS.gene034173.t1 AT3G02740 27.424 361 228 16 37 374 16 365 5.58e-25 105
MS.gene034173.t1 AT5G02190 26.525 377 218 16 40 373 82 442 3.20e-24 103
MS.gene034173.t1 AT1G65240 26.344 372 232 16 29 374 72 427 1.72e-23 102
MS.gene034173.t1 AT5G43100 26.486 370 227 15 27 374 72 418 3.60e-23 100
MS.gene034173.t1 AT1G09750 25.840 387 248 13 11 373 77 448 7.97e-23 99.8
MS.gene034173.t1 AT5G43100 26.486 370 227 15 27 374 72 418 8.10e-23 100
MS.gene034173.t1 AT5G45120 27.273 407 221 19 31 373 83 478 1.74e-22 99.0
MS.gene034173.t1 AT1G66180 24.129 373 239 13 31 375 72 428 2.10e-22 98.6
MS.gene034173.t1 AT5G37540 25.333 375 232 14 31 373 80 438 1.60e-21 95.9
MS.gene034173.t1 AT3G50050 24.251 367 241 12 28 375 90 438 6.43e-19 89.0
MS.gene034173.t1 AT1G05840 25.401 374 239 15 29 374 78 439 5.08e-18 85.9
MS.gene034173.t1 AT4G12920 27.424 361 213 19 31 373 58 387 7.09e-18 84.7
MS.gene034173.t1 AT5G07030 28.226 372 208 17 31 374 115 455 5.46e-17 82.4
MS.gene034173.t1 AT3G52500 27.543 403 213 21 29 374 88 468 1.02e-16 81.6
MS.gene034173.t1 AT1G08210 26.064 376 232 19 29 374 82 441 2.09e-15 77.8
MS.gene034173.t1 AT1G08210 26.064 376 232 19 29 374 116 475 2.32e-15 77.8
MS.gene034173.t1 AT1G08210 26.064 376 232 19 29 374 130 489 3.51e-15 77.4
MS.gene034173.t1 AT5G24820 22.656 384 260 11 18 377 33 403 1.46e-14 75.1
MS.gene034173.t1 AT1G77480 26.463 393 218 22 23 375 60 421 2.43e-14 74.7
MS.gene034173.t1 AT1G77480 26.463 393 218 22 23 375 60 421 2.67e-14 74.3
MS.gene034173.t1 AT3G51350 25.956 366 223 16 36 373 107 452 3.62e-14 74.3
MS.gene034173.t1 AT2G17760 25.620 363 223 17 36 373 109 449 2.69e-13 71.6
MS.gene034173.t1 AT3G42550 21.703 364 214 10 31 374 78 390 5.17e-13 70.5
MS.gene034173.t1 AT1G08210 27.742 310 187 16 29 315 82 377 7.24e-13 70.1
MS.gene034173.t1 AT5G10080 25.946 370 223 21 37 375 106 455 6.27e-12 67.4

Find 80 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGGTATTTATCATAATT+TGG 0.131312 4.3:-87536822 MS.gene034173:CDS
TGAAGGCATCTTTGGGAATT+TGG 0.131735 4.3:-87536588 MS.gene034173:CDS
GGAGAAGCTGCTGTAGTTTC+TGG 0.166013 4.3:-87537016 MS.gene034173:CDS
CGACTCAATGAAGGCATCTT+TGG 0.201942 4.3:-87536596 MS.gene034173:CDS
TTAAAATGTGCAGTGATTAT+AGG 0.219892 4.3:+87536701 None:intergenic
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.3:-87537076 MS.gene034173:CDS
CCTAAAACTGTGATAGGTTT+TGG 0.228752 4.3:-87537199 MS.gene034173:CDS
TCGACGAGCAAACTCAATTT+TGG 0.229402 4.3:-87537037 MS.gene034173:CDS
ACCCCGCCATTTAAGTTATA+TGG 0.232605 4.3:-87537502 MS.gene034173:CDS
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.3:-87537077 MS.gene034173:CDS
AGTGGGGATCACATTGGATT+TGG 0.281086 4.3:+87537558 None:intergenic
ATAATACCATATAACTTAAA+TGG 0.291745 4.3:+87537496 None:intergenic
TCATTTCCTAAAACTGTGAT+AGG 0.301005 4.3:-87537205 MS.gene034173:CDS
TGCTGATGGGGTTGTCTGTT+TGG 0.306514 4.3:-87536633 MS.gene034173:CDS
CGTGTCTCTTATAAATCAAT+TGG 0.312361 4.3:-87537113 MS.gene034173:CDS
GTGGGGATCACATTGGATTT+GGG 0.335142 4.3:+87537559 None:intergenic
AACAAAGTCTGCACTCAATA+TGG 0.340167 4.3:+87536491 None:intergenic
AATCAATTGGGTTCATCAAT+TGG 0.341653 4.3:-87537100 MS.gene034173:CDS
GGTAACATCATGGTTGATTC+AGG 0.351027 4.3:-87536866 MS.gene034173:CDS
CAAAGGTTTAAATAACCATT+TGG 0.354673 4.3:+87536755 None:intergenic
TGGATTTGGGTGTGTTAGTA+AGG 0.374259 4.3:+87537572 None:intergenic
ATACAGGTAGCGACATAGTT+TGG 0.378441 4.3:-87537471 MS.gene034173:CDS
GACTCAATGAAGGCATCTTT+GGG 0.379134 4.3:-87536595 MS.gene034173:CDS
CATAAGAAACTCACCGTTAG+TGG 0.387341 4.3:+87537540 None:intergenic
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.3:-87537139 MS.gene034173:CDS
GTAATGATCGACACGGTTAA+TGG 0.392714 4.3:+87537606 None:intergenic
CAATCAAATGGTAGTGTTGA+TGG 0.396941 4.3:-87536887 MS.gene034173:CDS
AGCTCTGGTGTGGTTGGCCT+TGG 0.398793 4.3:-87537145 MS.gene034173:CDS
ACCTTTGTTTCAGTTGCTGA+TGG 0.408371 4.3:-87536647 MS.gene034173:CDS
GATTAAAGATAGAAGTCGTT+TGG 0.412526 4.3:+87537416 None:intergenic
ATCACAAGGAGATCTTAGTT+TGG 0.415779 4.3:-87537266 MS.gene034173:CDS
CCTTTGTTTCAGTTGCTGAT+GGG 0.416283 4.3:-87536646 MS.gene034173:CDS
TTATATGGTATTATTGATAC+AGG 0.423817 4.3:-87537487 MS.gene034173:CDS
TTTGGCTCAACAAAATTTGT+TGG 0.424190 4.3:-87536570 MS.gene034173:CDS
GGAATTAGCTGTGGTAGAAT+CGG 0.458402 4.3:-87536801 MS.gene034173:CDS
TACCATATAACTTAAATGGC+GGG 0.463919 4.3:+87537500 None:intergenic
GCTCAACAAAATTTGTTGGT+TGG 0.467228 4.3:-87536566 MS.gene034173:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.3:-87537238 MS.gene034173:CDS
TGGCGGGGTACCGACTGAAT+AGG 0.482486 4.3:+87537516 None:intergenic
GGTTAGACTAAAGCGTGTTA+AGG 0.486147 4.3:-87536780 MS.gene034173:CDS
GGCAAGAGCTCTGGTGTGGT+TGG 0.491732 4.3:-87537151 MS.gene034173:CDS
GTGTCTCTTATAAATCAATT+GGG 0.493038 4.3:-87537112 MS.gene034173:CDS
CTTTGTTTCAGTTGCTGATG+GGG 0.494820 4.3:-87536645 MS.gene034173:CDS
TCATTTGAAGGCAAGAGCTC+TGG 0.501352 4.3:-87537160 MS.gene034173:CDS
AGTCTGCACTCAATATGGTT+TGG 0.515365 4.3:+87536496 None:intergenic
TTGTAACTTGATGATTTCAA+AGG 0.516263 4.3:+87537394 None:intergenic
GTCCCATCTCGACTCAATGA+AGG 0.517181 4.3:-87536605 MS.gene034173:CDS
GAATTTGCAAAGCAATCAAA+TGG 0.522533 4.3:-87536899 MS.gene034173:CDS
ATGCCTTCATTGAGTCGAGA+TGG 0.524151 4.3:+87536602 None:intergenic
ACATTGACAACTGTACCATC+TGG 0.525694 4.3:-87536839 MS.gene034173:CDS
CCCATCAGCAACTGAAACAA+AGG 0.528788 4.3:+87536646 None:intergenic
GCTGCTGTAGTTTCTGGTCA+TGG 0.536172 4.3:-87537010 MS.gene034173:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.3:-87536935 MS.gene034173:CDS
AAGCGTGTTAAGGATCCAAA+TGG 0.542262 4.3:-87536770 MS.gene034173:CDS
ACGGTTAATGGAACGACGCA+TGG 0.550865 4.3:+87537618 None:intergenic
TGCCTTCATTGAGTCGAGAT+GGG 0.557467 4.3:+87536603 None:intergenic
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.3:-87537075 MS.gene034173:CDS
TATGGTTTGGTACAATCAGT+AGG 0.559170 4.3:+87536509 None:intergenic
ACCATATAACTTAAATGGCG+GGG 0.576438 4.3:+87537501 None:intergenic
ACCGTTAGTGGGGATCACAT+TGG 0.582419 4.3:+87537552 None:intergenic
CAATTGATTTATAAGAGACA+CGG 0.582532 4.3:+87537114 None:intergenic
TTGATGTAACAGAATAACAA+AGG 0.586188 4.3:+87536738 None:intergenic
ATAAGAAACTCACCGTTAGT+GGG 0.587177 4.3:+87537541 None:intergenic
TGGTTGTAACAGTGTTCACA+AGG 0.587638 4.3:+87537436 None:intergenic
CTCGTCGAGTTAGACGACTC+TGG 0.588889 4.3:+87537052 None:intergenic
ATACCATATAACTTAAATGG+CGG 0.593415 4.3:+87537499 None:intergenic
TCCAATGTGATCCCCACTAA+CGG 0.595512 4.3:-87537553 MS.gene034173:CDS
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.3:-87537097 MS.gene034173:CDS
GAGACACGGGTCCATTTCCA+AGG 0.606926 4.3:+87537128 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.3:+87537225 None:intergenic
TCATAATTTGGAATTAGCTG+TGG 0.617309 4.3:-87536810 MS.gene034173:CDS
TTTCTTATGACCTATTCAGT+CGG 0.625475 4.3:-87537526 MS.gene034173:CDS
AAATTATGATAAATACCAGA+TGG 0.639824 4.3:+87536824 None:intergenic
AATTGATTTATAAGAGACAC+GGG 0.646628 4.3:+87537115 None:intergenic
TAAGAAACTCACCGTTAGTG+GGG 0.653160 4.3:+87537542 None:intergenic
TGAAGGCAAGAGCTCTGGTG+TGG 0.663721 4.3:-87537155 MS.gene034173:CDS
TAGTGTTGATGGTAACATCA+TGG 0.667211 4.3:-87536876 MS.gene034173:CDS
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.3:+87537222 None:intergenic
GTTTGTAGTAATGATCGACA+CGG 0.708983 4.3:+87537599 None:intergenic
GATGATAGATCAATATCACA+AGG 0.717243 4.3:-87537280 MS.gene034173:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAAGTAAAAAATTTGT+AGG + chr4.3:87537167-87537186 None:intergenic 10.0%
!! ATAATACCATATAACTTAAA+TGG + chr4.3:87536628-87536647 None:intergenic 15.0%
!! AAATTATGATAAATACCAGA+TGG + chr4.3:87537300-87537319 None:intergenic 20.0%
!! ATCTGGTATTTATCATAATT+TGG - chr4.3:87537299-87537318 MS.gene034173:CDS 20.0%
!! TTATATGGTATTATTGATAC+AGG - chr4.3:87536634-87536653 MS.gene034173:CDS 20.0%
!!! AAAAATACAATGTCATTTGA+AGG - chr4.3:87536949-87536968 MS.gene034173:CDS 20.0%
!!! AGTTTTTTTTTTACTACAAG+AGG + chr4.3:87536800-87536819 None:intergenic 20.0%
! ATACCATATAACTTAAATGG+CGG + chr4.3:87536625-87536644 None:intergenic 25.0%
! CAAAGGTTTAAATAACCATT+TGG + chr4.3:87537369-87537388 None:intergenic 25.0%
! GTGTCTCTTATAAATCAATT+GGG - chr4.3:87537009-87537028 MS.gene034173:CDS 25.0%
! TTAAAATGTGCAGTGATTAT+AGG + chr4.3:87537423-87537442 None:intergenic 25.0%
! TTGATGTAACAGAATAACAA+AGG + chr4.3:87537386-87537405 None:intergenic 25.0%
!! AATTGATTTATAAGAGACAC+GGG + chr4.3:87537009-87537028 None:intergenic 25.0%
!! ATAATCACTGCACATTTTAA+AGG - chr4.3:87537423-87537442 MS.gene034173:CDS 25.0%
!! CAATTGATTTATAAGAGACA+CGG + chr4.3:87537010-87537029 None:intergenic 25.0%
!! TTGTAACTTGATGATTTCAA+AGG + chr4.3:87536730-87536749 None:intergenic 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.3:87536902-87536921 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG - chr4.3:87537021-87537040 MS.gene034173:CDS 30.0%
CGTGTCTCTTATAAATCAAT+TGG - chr4.3:87537008-87537027 MS.gene034173:CDS 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.3:87537046-87537065 MS.gene034173:CDS 30.0%
GAATTTGCAAAGCAATCAAA+TGG - chr4.3:87537222-87537241 MS.gene034173:CDS 30.0%
GATGATAGATCAATATCACA+AGG - chr4.3:87536841-87536860 MS.gene034173:CDS 30.0%
GATTAAAGATAGAAGTCGTT+TGG + chr4.3:87536708-87536727 None:intergenic 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.3:87537045-87537064 MS.gene034173:CDS 30.0%
TACCATATAACTTAAATGGC+GGG + chr4.3:87536624-87536643 None:intergenic 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr4.3:87536916-87536935 MS.gene034173:CDS 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.3:87537044-87537063 MS.gene034173:CDS 30.0%
TTTCTTATGACCTATTCAGT+CGG - chr4.3:87536595-87536614 MS.gene034173:CDS 30.0%
! TCATAATTTGGAATTAGCTG+TGG - chr4.3:87537311-87537330 MS.gene034173:CDS 30.0%
! TTTGGCTCAACAAAATTTGT+TGG - chr4.3:87537551-87537570 MS.gene034173:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.3:87537186-87537205 MS.gene034173:CDS 30.0%
!!! TGTAGGTCTTTTTTCACTAA+AGG + chr4.3:87537150-87537169 None:intergenic 30.0%
ACCATATAACTTAAATGGCG+GGG + chr4.3:87536623-87536642 None:intergenic 35.0%
ATAAGAAACTCACCGTTAGT+GGG + chr4.3:87536583-87536602 None:intergenic 35.0%
ATCACAAGGAGATCTTAGTT+TGG - chr4.3:87536855-87536874 MS.gene034173:CDS 35.0%
GTTTGTAGTAATGATCGACA+CGG + chr4.3:87536525-87536544 None:intergenic 35.0%
! CAATCAAATGGTAGTGTTGA+TGG - chr4.3:87537234-87537253 MS.gene034173:CDS 35.0%
! CCAAAACCTATCACAGTTTT+AGG + chr4.3:87536925-87536944 None:intergenic 35.0%
! CCTAAAACTGTGATAGGTTT+TGG - chr4.3:87536922-87536941 MS.gene034173:CDS 35.0%
!! GCTCAACAAAATTTGTTGGT+TGG - chr4.3:87537555-87537574 MS.gene034173:CDS 35.0%
!! TAGTGTTGATGGTAACATCA+TGG - chr4.3:87537245-87537264 MS.gene034173:CDS 35.0%
!! TATGGTTTGGTACAATCAGT+AGG + chr4.3:87537615-87537634 None:intergenic 35.0%
AAGCGTGTTAAGGATCCAAA+TGG - chr4.3:87537351-87537370 MS.gene034173:CDS 40.0%
ACATTGACAACTGTACCATC+TGG - chr4.3:87537282-87537301 MS.gene034173:CDS 40.0%
ATACAGGTAGCGACATAGTT+TGG - chr4.3:87536650-87536669 MS.gene034173:CDS 40.0%
CAATTGGGTTCATCAATTGG+TGG - chr4.3:87537024-87537043 MS.gene034173:CDS 40.0%
CATAAGAAACTCACCGTTAG+TGG + chr4.3:87536584-87536603 None:intergenic 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.3:87536883-87536902 MS.gene034173:CDS 40.0%
GGAATTAGCTGTGGTAGAAT+CGG - chr4.3:87537320-87537339 MS.gene034173:CDS 40.0%
GGTTAGACTAAAGCGTGTTA+AGG - chr4.3:87537341-87537360 MS.gene034173:CDS 40.0%
GTAATGATCGACACGGTTAA+TGG + chr4.3:87536518-87536537 None:intergenic 40.0%
TAAGAAACTCACCGTTAGTG+GGG + chr4.3:87536582-87536601 None:intergenic 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.3:87536899-87536918 None:intergenic 40.0%
TGGATTTGGGTGTGTTAGTA+AGG + chr4.3:87536552-87536571 None:intergenic 40.0%
TGGTTGTAACAGTGTTCACA+AGG + chr4.3:87536688-87536707 None:intergenic 40.0%
! ACCCCGCCATTTAAGTTATA+TGG - chr4.3:87536619-87536638 MS.gene034173:CDS 40.0%
! ACCTTTGTTTCAGTTGCTGA+TGG - chr4.3:87537474-87537493 MS.gene034173:CDS 40.0%
! CCTTTGTTTCAGTTGCTGAT+GGG - chr4.3:87537475-87537494 MS.gene034173:CDS 40.0%
! CTTTGTTTCAGTTGCTGATG+GGG - chr4.3:87537476-87537495 MS.gene034173:CDS 40.0%
! GGTAACATCATGGTTGATTC+AGG - chr4.3:87537255-87537274 MS.gene034173:CDS 40.0%
! TCGACGAGCAAACTCAATTT+TGG - chr4.3:87537084-87537103 MS.gene034173:CDS 40.0%
!! GACTCAATGAAGGCATCTTT+GGG - chr4.3:87537526-87537545 MS.gene034173:CDS 40.0%
!! TGAAGGCATCTTTGGGAATT+TGG - chr4.3:87537533-87537552 MS.gene034173:CDS 40.0%
AGTGGGGATCACATTGGATT+TGG + chr4.3:87536566-87536585 None:intergenic 45.0%
ATGCCTTCATTGAGTCGAGA+TGG + chr4.3:87537522-87537541 None:intergenic 45.0%
CCCATCAGCAACTGAAACAA+AGG + chr4.3:87537478-87537497 None:intergenic 45.0%
GTGGGGATCACATTGGATTT+GGG + chr4.3:87536565-87536584 None:intergenic 45.0%
TCATTTGAAGGCAAGAGCTC+TGG - chr4.3:87536961-87536980 MS.gene034173:CDS 45.0%
TCCAATGTGATCCCCACTAA+CGG - chr4.3:87536568-87536587 MS.gene034173:CDS 45.0%
TGCCTTCATTGAGTCGAGAT+GGG + chr4.3:87537521-87537540 None:intergenic 45.0%
!! CGACTCAATGAAGGCATCTT+TGG - chr4.3:87537525-87537544 MS.gene034173:CDS 45.0%
ACCGTTAGTGGGGATCACAT+TGG + chr4.3:87536572-87536591 None:intergenic 50.0%
GGAGAAGCTGCTGTAGTTTC+TGG - chr4.3:87537105-87537124 MS.gene034173:CDS 50.0%
GTCCCATCTCGACTCAATGA+AGG - chr4.3:87537516-87537535 MS.gene034173:CDS 50.0%
TGCTGATGGGGTTGTCTGTT+TGG - chr4.3:87537488-87537507 MS.gene034173:CDS 50.0%
!! GCTGCTGTAGTTTCTGGTCA+TGG - chr4.3:87537111-87537130 MS.gene034173:CDS 50.0%
CTCGTCGAGTTAGACGACTC+TGG + chr4.3:87537072-87537091 None:intergenic 55.0%
GAGACACGGGTCCATTTCCA+AGG + chr4.3:87536996-87537015 None:intergenic 55.0%
! TGAAGGCAAGAGCTCTGGTG+TGG - chr4.3:87536966-87536985 MS.gene034173:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.3:87536982-87537001 MS.gene034173:CDS 55.0%
! GGCAAGAGCTCTGGTGTGGT+TGG - chr4.3:87536970-87536989 MS.gene034173:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.3:87536976-87536995 MS.gene034173:CDS 60.0%
!! TGGCGGGGTACCGACTGAAT+AGG + chr4.3:87536608-87536627 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 87536505 87537638 87536505 ID=MS.gene034173
chr4.3 mRNA 87536505 87537638 87536505 ID=MS.gene034173.t1;Parent=MS.gene034173
chr4.3 exon 87536505 87537638 87536505 ID=MS.gene034173.t1.exon1;Parent=MS.gene034173.t1
chr4.3 CDS 87536505 87537638 87536505 ID=cds.MS.gene034173.t1;Parent=MS.gene034173.t1
Gene Sequence

>MS.gene034173

ATGCGTCGTTCCATTAACCGTGTCGATCATTACTACAAACATTTCCTTACTAACACACCCAAATCCAATGTGATCCCCACTAACGGTGAGTTTCTTATGACCTATTCAGTCGGTACCCCGCCATTTAAGTTATATGGTATTATTGATACAGGTAGCGACATAGTTTGGCTTCAATCTAAGCCTTGTGAACACTGTTACAACCAAACGACTTCTATCTTTAATCCTTTGAAATCATCAAGTTACAAAAACAATATTGCTTGTTTGTCTAAAAAATGTAAGTCTTTTGAATCTACCTCTTGTAGTAAAAAAAAAACTTGCGAATATACTATTATATATGATGATAGATCAATATCACAAGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACTGTGATAGGTTTTGGACATAAAAATACAATGTCATTTGAAGGCAAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCCGTGTCTCTTATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCAGAGTCGTCTAACTCGACGAGCAAACTCAATTTTGGAGAAGCTGCTGTAGTTTCTGGTCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCTACAAATTTTTTACTTAATAACATTGAAAGCATTTAGTGTTGGAAATAAAAGAGTAGAATTTGCAAAGCAATCAAATGGTAGTGTTGATGGTAACATCATGGTTGATTCAGGTACAACATTGACAACTGTACCATCTGGTATTTATCATAATTTGGAATTAGCTGTGGTAGAATCGGTTAGACTAAAGCGTGTTAAGGATCCAAATGGTTATTTAAACCTTTGTTATTCTGTTACATCAAAAAAATATGATTTTCCTATAATCACTGCACATTTTAAAGGTGCAGATGTTAAGTTGCATTCTATTAGCACCTTTGTTTCAGTTGCTGATGGGGTTGTCTGTTTGGCTCTTGTCCCATCTCGACTCAATGAAGGCATCTTTGGGAATTTGGCTCAACAAAATTTGTTGGTTGGTTATGATATTCAACAAAAAATCATGTCTTTTAAGCCTACTGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene034173.t1

MRRSINRVDHYYKHFLTNTPKSNVIPTNGEFLMTYSVGTPPFKLYGIIDTGSDIVWLQSKPCEHCYNQTTSIFNPLKSSSYKNNIACLSKKCKSFESTSCSKKKTCEYTIIYDDRSISQGDLSLETLTLDSTSGSSISFPKTVIGFGHKNTMSFEGKSSGVVGLGNGPVSLINQLGSSIGGKFSYCLGPESSNSTSKLNFGEAAVVSGHGVVSTPLVKKDLQIFYLITLKAFSVGNKRVEFAKQSNGSVDGNIMVDSGTTLTTVPSGIYHNLELAVVESVRLKRVKDPNGYLNLCYSVTSKKYDFPIITAHFKGADVKLHSISTFVSVADGVVCLALVPSRLNEGIFGNLAQQNLLVGYDIQQKIMSFKPTDCTKPY