Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03677.t1 | XP_024633839.1 | 96.1 | 490 | 19 | 0 | 1 | 490 | 1 | 490 | 1.60E-257 | 898.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03677.t1 | O48791 | 71.0 | 490 | 138 | 2 | 1 | 486 | 1 | 490 | 9.7e-187 | 654.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03677.t1 | A0A396IPJ6 | 96.1 | 490 | 19 | 0 | 1 | 490 | 1 | 490 | 1.2e-257 | 898.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene58836 | MS.gene03677 | PPI |
| MS.gene017694 | MS.gene03677 | PPI |
| MS.gene38829 | MS.gene03677 | PPI |
| MS.gene013971 | MS.gene03677 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03677.t1 | MTR_3g045180 | 94.177 | 498 | 21 | 1 | 1 | 490 | 1 | 498 | 0.0 | 961 |
| MS.gene03677.t1 | MTR_5g088860 | 83.640 | 489 | 76 | 2 | 1 | 485 | 1 | 489 | 0.0 | 844 |
| MS.gene03677.t1 | MTR_5g088860 | 83.640 | 489 | 76 | 2 | 1 | 485 | 23 | 511 | 0.0 | 843 |
| MS.gene03677.t1 | MTR_7g089090 | 58.197 | 488 | 198 | 3 | 1 | 483 | 1 | 487 | 0.0 | 577 |
| MS.gene03677.t1 | MTR_6g079040 | 66.379 | 116 | 38 | 1 | 86 | 200 | 41 | 156 | 5.94e-38 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03677.t1 | AT2G26770 | 73.984 | 492 | 120 | 3 | 1 | 486 | 1 | 490 | 0.0 | 753 |
| MS.gene03677.t1 | AT2G26770 | 73.984 | 492 | 120 | 3 | 1 | 486 | 1 | 490 | 0.0 | 753 |
| MS.gene03677.t1 | AT2G26770 | 73.984 | 492 | 120 | 3 | 1 | 486 | 1 | 490 | 0.0 | 753 |
| MS.gene03677.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 47 | 516 | 0.0 | 524 |
| MS.gene03677.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 22 | 491 | 0.0 | 523 |
| MS.gene03677.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 34 | 503 | 0.0 | 523 |
| MS.gene03677.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 22 | 491 | 0.0 | 523 |
| MS.gene03677.t1 | AT3G56480 | 55.579 | 466 | 199 | 4 | 25 | 483 | 25 | 489 | 2.76e-180 | 514 |
| MS.gene03677.t1 | AT2G40820 | 53.403 | 382 | 175 | 2 | 104 | 483 | 1 | 381 | 1.17e-147 | 427 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCGGCGCAGGCACTATTT+TGG | 0.185288 | 3.4:+35613733 | MS.gene03677:CDS |
| CGATTTCCAACTTACAAAAT+TGG | 0.193646 | 3.4:+35606276 | MS.gene03677:CDS |
| GAGAGGATGTCTCGAACTTC+TGG | 0.270869 | 3.4:+35608714 | MS.gene03677:CDS |
| CCTCATTTATATGAATTAGA+AGG | 0.272590 | 3.4:+35610568 | MS.gene03677:CDS |
| TCCTTCATCGATAGCACTTT+CGG | 0.275084 | 3.4:-35606324 | None:intergenic |
| GATGGGTTGGAATCCTTAAA+AGG | 0.293122 | 3.4:+35607025 | MS.gene03677:CDS |
| CGTCCAACGTCAAAAGGTTC+TGG | 0.297621 | 3.4:-35612726 | None:intergenic |
| TGAACTCGAATAGAATTCTT+TGG | 0.297696 | 3.4:-35613506 | None:intergenic |
| CCTTCATCGATAGCACTTTC+GGG | 0.304015 | 3.4:-35606323 | None:intergenic |
| CCTCCTTCATTAATTGTTCC+AGG | 0.317717 | 3.4:-35609314 | None:intergenic |
| TGTACACGAAGCTATTTCTT+TGG | 0.342988 | 3.4:+35607075 | MS.gene03677:CDS |
| ATCACCCGAAAGTGCCAAAA+AGG | 0.350946 | 3.4:+35610681 | MS.gene03677:CDS |
| ATTGAACATTCTGAAAGTTC+GGG | 0.352004 | 3.4:-35610643 | None:intergenic |
| AATTGAACATTCTGAAAGTT+CGG | 0.353771 | 3.4:-35610644 | None:intergenic |
| AACACGAGCTTCAAGCATTA+AGG | 0.356617 | 3.4:+35609970 | MS.gene03677:CDS |
| GCGAGTGGCGGGAAGAAATA+AGG | 0.360346 | 3.4:+35607048 | MS.gene03677:CDS |
| CCACTCTTTGAACTGCTGCC+CGG | 0.360438 | 3.4:-35608671 | None:intergenic |
| GATGATGTGAGTCATTTGTC+TGG | 0.366669 | 3.4:-35613411 | None:intergenic |
| TTGCAGTTCAGTTAACTCAT+AGG | 0.368181 | 3.4:+35607907 | MS.gene03677:CDS |
| ACAAGGATGACGCGCAGTTT+TGG | 0.369059 | 3.4:+35606195 | MS.gene03677:CDS |
| TTCTGGGGCATAAACTGATT+TGG | 0.372582 | 3.4:-35612709 | None:intergenic |
| CACTCTTTGAACTGCTGCCC+GGG | 0.388647 | 3.4:-35608670 | None:intergenic |
| GTAGCCAAACCTGGTTCAAT+TGG | 0.395153 | 3.4:-35612807 | None:intergenic |
| TCCCACATTAAGTATATGAA+TGG | 0.411218 | 3.4:-35613443 | None:intergenic |
| CTTTGTGGAGTTCGAGGAGG+TGG | 0.411973 | 3.4:+35613705 | MS.gene03677:CDS |
| CTTCAGCTTTGTGGAGTTCG+AGG | 0.431632 | 3.4:+35613699 | MS.gene03677:intron |
| TGCTAAGAAACTTGTATATG+AGG | 0.435523 | 3.4:+35608614 | MS.gene03677:CDS |
| AGGTGGGAATGCTGCGGCGC+AGG | 0.440198 | 3.4:+35613722 | MS.gene03677:CDS |
| TTAGAAGGGACTGAAACATT+AGG | 0.443933 | 3.4:+35610583 | MS.gene03677:CDS |
| ACAAAAGAGAGTCCTTGCTT+TGG | 0.450797 | 3.4:-35613759 | None:intergenic |
| TCCTTAAAAGGGCGAGTGGC+GGG | 0.450878 | 3.4:+35607037 | MS.gene03677:CDS |
| AAAGAAGATAAGAGATGGGT+TGG | 0.458713 | 3.4:+35607012 | MS.gene03677:CDS |
| CATTTGAATCAGAGAGAGAA+AGG | 0.460633 | 3.4:+35613787 | MS.gene03677:CDS |
| TGAGAATGAAGCTTTGTAAA+GGG | 0.466239 | 3.4:+35613470 | MS.gene03677:CDS |
| AGTATATGAATGGATTCAGT+TGG | 0.467239 | 3.4:-35613433 | None:intergenic |
| GTCCAACGTCAAAAGGTTCT+GGG | 0.473124 | 3.4:-35612725 | None:intergenic |
| TAATTGCAGATTGTTCTAGC+TGG | 0.479576 | 3.4:+35613954 | MS.gene03677:intron |
| TGCAGTTCAGTTAACTCATA+GGG | 0.484553 | 3.4:+35607908 | MS.gene03677:CDS |
| ATGAGAATGAAGCTTTGTAA+AGG | 0.488741 | 3.4:+35613469 | MS.gene03677:CDS |
| TAGTAAATTTGAGAAGGGTT+TGG | 0.490035 | 3.4:+35606413 | MS.gene03677:CDS |
| ATCCATTCATATACTTAATG+TGG | 0.494470 | 3.4:+35613441 | MS.gene03677:CDS |
| CTCATTTATATGAATTAGAA+GGG | 0.497583 | 3.4:+35610569 | MS.gene03677:CDS |
| TGGATCTAATAATCAGATCA+TGG | 0.501525 | 3.4:+35606296 | MS.gene03677:CDS |
| CAAGTTGGTATTATTTCAGA+AGG | 0.506009 | 3.4:+35612756 | MS.gene03677:CDS |
| ATTCTATTCGAGTTCAATGC+AGG | 0.509177 | 3.4:+35613513 | MS.gene03677:CDS |
| GTCTCGAACTTCTGGAAACC+AGG | 0.510853 | 3.4:+35608722 | MS.gene03677:CDS |
| AGCAGTTCAAAGAGTGGAGG+AGG | 0.517740 | 3.4:+35608677 | MS.gene03677:CDS |
| AATATGCGTCCAACGTCAAA+AGG | 0.523688 | 3.4:-35612732 | None:intergenic |
| AAACTCTTTCAGAAACATGA+TGG | 0.524058 | 3.4:+35613988 | MS.gene03677:CDS |
| CTTGCTAGTAAATTTGAGAA+GGG | 0.528068 | 3.4:+35606408 | MS.gene03677:CDS |
| TTTCAGAATGTTCAATTCAG+TGG | 0.532345 | 3.4:+35610650 | MS.gene03677:CDS |
| CCCGAAAGTGCTATCGATGA+AGG | 0.534861 | 3.4:+35606323 | MS.gene03677:CDS |
| CCTTGCTAGTAAATTTGAGA+AGG | 0.543952 | 3.4:+35606407 | MS.gene03677:CDS |
| AGGCTCTTCAAGAGCATGAG+AGG | 0.544876 | 3.4:+35608697 | MS.gene03677:CDS |
| CCTTCTCAAATTTACTAGCA+AGG | 0.554709 | 3.4:-35606407 | None:intergenic |
| ATGATGTGAGTCATTTGTCT+GGG | 0.555872 | 3.4:-35613410 | None:intergenic |
| TTCAGTTAACTCATAGGGAA+GGG | 0.556887 | 3.4:+35607913 | MS.gene03677:CDS |
| ATGGGTTGGAATCCTTAAAA+GGG | 0.558486 | 3.4:+35607026 | MS.gene03677:CDS |
| CAGTTGGCAAGGGAAAAGAA+AGG | 0.562244 | 3.4:+35610532 | MS.gene03677:intron |
| TGCTGCTAAGTTGTCTGAAG+AGG | 0.562336 | 3.4:+35606440 | MS.gene03677:CDS |
| TCCATTCATATACTTAATGT+GGG | 0.569079 | 3.4:+35613442 | MS.gene03677:CDS |
| GGACCTGGAACAATTAATGA+AGG | 0.569122 | 3.4:+35609311 | MS.gene03677:intron |
| CAAACCTGGTTCAATTGGAC+CGG | 0.569962 | 3.4:-35612802 | None:intergenic |
| ACGCGAAACTGCACAGCTGT+TGG | 0.571348 | 3.4:+35606356 | MS.gene03677:CDS |
| ACCTGGTTCAATTGGACCGG+TGG | 0.575977 | 3.4:-35612799 | None:intergenic |
| CCTTCTAATTCATATAAATG+AGG | 0.579496 | 3.4:-35610568 | None:intergenic |
| GGCAGCAGTTCAAAGAGTGG+AGG | 0.581141 | 3.4:+35608674 | MS.gene03677:CDS |
| ATTAATGAAGGAGGTTCAAG+AGG | 0.588663 | 3.4:+35609323 | MS.gene03677:CDS |
| GTTCAGTTAACTCATAGGGA+AGG | 0.590453 | 3.4:+35607912 | MS.gene03677:CDS |
| TCAGTTAACTCATAGGGAAG+GGG | 0.591057 | 3.4:+35607914 | MS.gene03677:CDS |
| ATGATGAGAGATACCTTGCT+TGG | 0.592908 | 3.4:-35609372 | None:intergenic |
| ATCCTTAAAAGGGCGAGTGG+CGG | 0.594151 | 3.4:+35607036 | MS.gene03677:CDS |
| GGCAAGGGAAAAGAAAGGAG+AGG | 0.600428 | 3.4:+35610537 | MS.gene03677:CDS |
| CAGCTTTGTGGAGTTCGAGG+AGG | 0.602632 | 3.4:+35613702 | MS.gene03677:intron |
| ATGCAGCTATCATGCTTGCA+AGG | 0.603055 | 3.4:+35613811 | MS.gene03677:CDS |
| TTTGTGGAGTTCGAGGAGGT+GGG | 0.607486 | 3.4:+35613706 | MS.gene03677:CDS |
| ACCACCGGTCCAATTGAACC+AGG | 0.610618 | 3.4:+35612798 | MS.gene03677:CDS |
| GTTGGACGCATATTGCAAGT+TGG | 0.611694 | 3.4:+35612741 | MS.gene03677:CDS |
| TGACACTATGATGATGCATA+AGG | 0.614210 | 3.4:+35606218 | MS.gene03677:CDS |
| AGGGGAGCTAGTACAACAGA+AGG | 0.616255 | 3.4:+35607932 | MS.gene03677:CDS |
| CAGATCACACTGTCAACCAC+CGG | 0.621680 | 3.4:+35612783 | MS.gene03677:CDS |
| ATGAGTTAACTGAACTGCAA+GGG | 0.627954 | 3.4:-35607904 | None:intergenic |
| GCTAGTACAACAGAAGGCTG+AGG | 0.635371 | 3.4:+35607938 | MS.gene03677:CDS |
| TTGAACATTCTGAAAGTTCG+GGG | 0.637142 | 3.4:-35610642 | None:intergenic |
| TATGAGTTAACTGAACTGCA+AGG | 0.638190 | 3.4:-35607905 | None:intergenic |
| AATGCTGCATCAGCCAAGCA+AGG | 0.639243 | 3.4:+35609359 | MS.gene03677:CDS |
| TCGAGGAGGTGGGAATGCTG+CGG | 0.640974 | 3.4:+35613716 | MS.gene03677:CDS |
| CCGGGCAGCAGTTCAAAGAG+TGG | 0.642685 | 3.4:+35608671 | MS.gene03677:CDS |
| TCCAACGTCAAAAGGTTCTG+GGG | 0.650573 | 3.4:-35612724 | None:intergenic |
| GGAATCCTTAAAAGGGCGAG+TGG | 0.655039 | 3.4:+35607033 | MS.gene03677:CDS |
| CCTGGAACAATTAATGAAGG+AGG | 0.657650 | 3.4:+35609314 | MS.gene03677:CDS |
| TGAACATTCTGAAAGTTCGG+GGG | 0.661686 | 3.4:-35610641 | None:intergenic |
| CAGCTATCATGCTTGCAAGG+AGG | 0.667212 | 3.4:+35613814 | MS.gene03677:CDS |
| TATGATGATGCATAAGGACG+TGG | 0.671817 | 3.4:+35606224 | MS.gene03677:CDS |
| TGGTTTGGGAAACTATGTAG+AGG | 0.678930 | 3.4:+35612997 | MS.gene03677:CDS |
| GAAAGTGCTATCGATGAAGG+AGG | 0.682437 | 3.4:+35606326 | MS.gene03677:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 35606192 | 35614007 | 35606192 | ID=MS.gene03677 |
| chr3.4 | mRNA | 35606192 | 35614007 | 35606192 | ID=MS.gene03677.t1;Parent=MS.gene03677 |
| chr3.4 | exon | 35606192 | 35606461 | 35606192 | ID=MS.gene03677.t1.exon1;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35606192 | 35606461 | 35606192 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35606968 | 35607096 | 35606968 | ID=MS.gene03677.t1.exon2;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35606968 | 35607096 | 35606968 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35607897 | 35607989 | 35607897 | ID=MS.gene03677.t1.exon3;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35607897 | 35607989 | 35607897 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35608603 | 35608743 | 35608603 | ID=MS.gene03677.t1.exon4;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35608603 | 35608743 | 35608603 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35609312 | 35609380 | 35609312 | ID=MS.gene03677.t1.exon5;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35609312 | 35609380 | 35609312 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35609957 | 35610040 | 35609957 | ID=MS.gene03677.t1.exon6;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35609957 | 35610040 | 35609957 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35610535 | 35610715 | 35610535 | ID=MS.gene03677.t1.exon7;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35610535 | 35610715 | 35610535 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35612703 | 35612819 | 35612703 | ID=MS.gene03677.t1.exon8;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35612703 | 35612819 | 35612703 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35612993 | 35613051 | 35612993 | ID=MS.gene03677.t1.exon9;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35612993 | 35613051 | 35612993 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35613400 | 35613534 | 35613400 | ID=MS.gene03677.t1.exon10;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35613400 | 35613534 | 35613400 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35613705 | 35613854 | 35613705 | ID=MS.gene03677.t1.exon11;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35613705 | 35613854 | 35613705 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| chr3.4 | exon | 35613963 | 35614007 | 35613963 | ID=MS.gene03677.t1.exon12;Parent=MS.gene03677.t1 |
| chr3.4 | CDS | 35613963 | 35614007 | 35613963 | ID=cds.MS.gene03677.t1;Parent=MS.gene03677.t1 |
| Gene Sequence |
| Protein sequence |
