AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036860.t1 MTR_4g113270 98.844 346 3 1 1 346 1 345 0.0 679
MS.gene036860.t1 MTR_4g113270 98.824 340 3 1 1 340 1 339 0.0 668
MS.gene036860.t1 MTR_1g100860 50.158 317 149 3 15 328 41 351 3.67e-98 295
MS.gene036860.t1 MTR_4g018770 49.527 317 151 3 15 328 35 345 4.35e-98 295
MS.gene036860.t1 MTR_4g018770 49.412 255 120 3 77 328 5 253 2.44e-76 236
MS.gene036860.t1 MTR_1g100860 49.804 255 119 3 77 328 5 253 2.78e-76 236
MS.gene036860.t1 MTR_3g463360 24.242 297 206 6 19 304 108 396 1.06e-12 68.9
MS.gene036860.t1 MTR_3g109070 27.692 260 164 7 18 267 20 265 2.24e-12 67.4
MS.gene036860.t1 MTR_3g109070 27.692 260 164 7 18 267 20 265 3.22e-12 66.6
MS.gene036860.t1 MTR_3g096310 23.102 303 218 5 18 311 45 341 7.17e-12 66.2
MS.gene036860.t1 MTR_3g109070 28.218 202 126 5 18 210 20 211 2.72e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036860.t1 AT5G19980 71.594 345 91 2 2 346 3 340 1.85e-169 475
MS.gene036860.t1 AT1G76340 48.148 324 166 2 15 337 37 359 9.93e-100 299
MS.gene036860.t1 AT4G31600 26.848 257 170 5 18 267 19 264 3.73e-13 69.7
MS.gene036860.t1 AT2G13650 22.896 297 212 5 18 304 38 327 8.66e-12 65.9
MS.gene036860.t1 AT2G13650 22.896 297 212 5 18 304 38 327 8.66e-12 65.9
MS.gene036860.t1 AT4G31600 27.136 199 132 3 18 210 19 210 1.32e-11 65.1

Find 64 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGGTTGTTATTCTTGC+TGG 0.233454 8.4:+15189659 MS.gene036860:CDS
GCTACCGCGTTTACCGTTAC+TGG 0.269983 8.4:+15190025 MS.gene036860:CDS
GGTTTGTTGATTAGTTTCTT+TGG 0.270396 8.4:+15189977 MS.gene036860:CDS
GAAACCCTAATTTACCTAAA+AGG 0.287655 8.4:-15189427 None:intergenic
TTACACTCACGGCTTATTCT+TGG 0.301434 8.4:+15189714 MS.gene036860:CDS
TGAGTCTTGGGTTGAATACT+TGG 0.340183 8.4:+15189795 MS.gene036860:CDS
AATAACAACCAAGGACAAAA+AGG 0.342091 8.4:-15189651 None:intergenic
TACACTCACGGCTTATTCTT+GGG 0.343245 8.4:+15189715 MS.gene036860:CDS
GGTTATGTTGCTACTGATTC+CGG 0.348332 8.4:+15189689 MS.gene036860:CDS
CTACCGCGTTTACCGTTACT+GGG 0.359242 8.4:+15190026 MS.gene036860:CDS
TGGCGATTAATGTGACGATT+TGG 0.360897 8.4:+15190071 MS.gene036860:CDS
GTTTCGTTGTCTTGTGTGTT+TGG 0.368137 8.4:+15189956 MS.gene036860:CDS
GATGGTTTATATTAAGCATA+TGG 0.374607 8.4:+15189769 MS.gene036860:CDS
GCATGCTAGTCCAGCTGGTT+TGG 0.378044 8.4:+15190099 MS.gene036860:CDS
AAGCATATGGTTATGAGTCT+TGG 0.383947 8.4:+15189782 MS.gene036860:CDS
AGCATATGGTTATGAGTCTT+GGG 0.397212 8.4:+15189783 MS.gene036860:CDS
GACTGGAAGTGGTTCGCAAC+AGG 0.414839 8.4:+15190171 MS.gene036860:CDS
GTTATTCTTGCTGGTGCTGT+TGG 0.419854 8.4:+15189668 MS.gene036860:CDS
GGCGATTAATGTGACGATTT+GGG 0.432854 8.4:+15190072 MS.gene036860:CDS
GATGTTGAAAGTAGTCTTGT+TGG 0.433983 8.4:+15190220 MS.gene036860:CDS
GCCACAAGTGGCTTAGTAAT+CGG 0.437849 8.4:+15189290 MS.gene036860:CDS
GGGAAGAATTTCTTAGCGAT+TGG 0.443284 8.4:-15189473 None:intergenic
AAGAAGAAAACGAGTGCTGC+AGG 0.450306 8.4:-15189494 None:intergenic
GAGTCTTGGGTTGAATACTT+GGG 0.451840 8.4:+15189796 MS.gene036860:CDS
CTCGCTGATACTGCGTTTCG+CGG 0.463897 8.4:+15189605 MS.gene036860:CDS
CAGCAATCGGTGACTGGAAG+TGG 0.465887 8.4:+15190160 MS.gene036860:CDS
GGGTGTTCTTTATCAGCAAT+CGG 0.471127 8.4:+15190147 MS.gene036860:CDS
CTTTATCAGCAATCGGTGAC+TGG 0.474553 8.4:+15190154 MS.gene036860:CDS
TGGTTCGCAACAGGCGGTTG+TGG 0.476669 8.4:+15190180 MS.gene036860:CDS
GTTAGCGTGACGAAGGAGAT+TGG 0.477146 8.4:-15189534 None:intergenic
ATAAGCCGTGAGTGTAAAAC+CGG 0.495535 8.4:-15189708 None:intergenic
ACTGCCTTGAGATCGAGTAC+TGG 0.496102 8.4:+15189902 MS.gene036860:CDS
TGAAGATGAGAGTGAAGGAA+AGG 0.502584 8.4:+15190255 MS.gene036860:CDS
CAATATCTCACTTCTTCACT+CGG 0.505402 8.4:+15189398 MS.gene036860:CDS
CTGCCTTGAGATCGAGTACT+GGG 0.507997 8.4:+15189903 MS.gene036860:CDS
AGTCTTGGGTTGAATACTTG+GGG 0.509311 8.4:+15189797 MS.gene036860:CDS
GATAAGCATGCTAGTCCAGC+TGG 0.512847 8.4:+15190094 MS.gene036860:CDS
ATAAACCATCTCAGTAGTAA+TGG 0.529935 8.4:-15189756 None:intergenic
GAAGATGAGAGTGAAGGAAA+GGG 0.531374 8.4:+15190256 MS.gene036860:CDS
AGCAAACAAACCAAACCAGC+TGG 0.537823 8.4:-15190109 None:intergenic
TTAGCGATTGGAATAGTGAA+AGG 0.542673 8.4:-15189461 None:intergenic
GGTTACCATTACTACTGAGA+TGG 0.551400 8.4:+15189751 MS.gene036860:CDS
TGTAACGCAGTTAAAAGACC+TGG 0.556729 8.4:-15189377 None:intergenic
TCAGCGAGCGCAACAAGAAG+AGG 0.567028 8.4:-15189590 None:intergenic
CAGCGAGCGCAACAAGAAGA+GGG 0.576233 8.4:-15189589 None:intergenic
CAACAACACTACGCCACAAG+TGG 0.587960 8.4:+15189278 MS.gene036860:CDS
GAAAGTAGTCTTGTTGGTGA+TGG 0.589182 8.4:+15190226 MS.gene036860:CDS
ACCAGCAAGAATAACAACCA+AGG 0.595444 8.4:-15189660 None:intergenic
AAGGTAAAATTAGACGGAGA+GGG 0.604146 8.4:-15189632 None:intergenic
CTTATTCACAACCCCAGTAA+CGG 0.607538 8.4:-15190038 None:intergenic
AAAGGTAAAATTAGACGGAG+AGG 0.624222 8.4:-15189633 None:intergenic
GTGACGAAGGAGATTGGTGT+TGG 0.632002 8.4:-15189528 None:intergenic
ACTCCCAGTACTCGATCTCA+AGG 0.635928 8.4:-15189906 None:intergenic
ACCGATTACTAAGCCACTTG+TGG 0.637733 8.4:-15189291 None:intergenic
TATCAACGTTAGCGTGACGA+AGG 0.639770 8.4:-15189541 None:intergenic
ATTACTCAATTCAACTACCC+AGG 0.649152 8.4:+15189359 MS.gene036860:CDS
GTAACGCAGTTAAAAGACCT+GGG 0.655430 8.4:-15189376 None:intergenic
AGAAGAAAACGAGTGCTGCA+GGG 0.656660 8.4:-15189493 None:intergenic
TGGAAGTGGTTCGCAACAGG+CGG 0.657769 8.4:+15190174 MS.gene036860:CDS
TGTGAATAAGTTTCTGACTG+TGG 0.673099 8.4:+15190051 MS.gene036860:CDS
AGCGAGCGCAACAAGAAGAG+GGG 0.681654 8.4:-15189588 None:intergenic
GATTCTGAAGATGAGAGTGA+AGG 0.689840 8.4:+15190250 MS.gene036860:CDS
TACCGCGTTTACCGTTACTG+GGG 0.705827 8.4:+15190027 MS.gene036860:CDS
AACCCCAGTAACGGTAAACG+CGG 0.739549 8.4:-15190029 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACCTTTTAGGTAAATTA+GGG + chr8.4:15189423-15189442 MS.gene036860:CDS 20.0%
! GACAAAAAGGTAAAATTAGA+CGG - chr8.4:15189641-15189660 None:intergenic 25.0%
! GATGGTTTATATTAAGCATA+TGG + chr8.4:15189769-15189788 MS.gene036860:CDS 25.0%
!! TAATTTTACCTTTTTGTCCT+TGG + chr8.4:15189643-15189662 MS.gene036860:CDS 25.0%
!!! GTTTACCTTTTAGGTAAATT+AGG + chr8.4:15189422-15189441 MS.gene036860:CDS 25.0%
!!! GTTTGCTTTTTACGATTATT+GGG + chr8.4:15190125-15190144 MS.gene036860:CDS 25.0%
!!! TGTTTGCTTTTTACGATTAT+TGG + chr8.4:15190124-15190143 MS.gene036860:CDS 25.0%
!!! TTTGCTTTTTACGATTATTG+GGG + chr8.4:15190126-15190145 MS.gene036860:CDS 25.0%
AATAACAACCAAGGACAAAA+AGG - chr8.4:15189654-15189673 None:intergenic 30.0%
ATAAACCATCTCAGTAGTAA+TGG - chr8.4:15189759-15189778 None:intergenic 30.0%
GAAACCCTAATTTACCTAAA+AGG - chr8.4:15189430-15189449 None:intergenic 30.0%
!! GGTTTGTTGATTAGTTTCTT+TGG + chr8.4:15189977-15189996 MS.gene036860:CDS 30.0%
!!! TTGCTTTTTACGATTATTGG+GGG + chr8.4:15190127-15190146 MS.gene036860:CDS 30.0%
AAAGGTAAAATTAGACGGAG+AGG - chr8.4:15189636-15189655 None:intergenic 35.0%
AAGCATATGGTTATGAGTCT+TGG + chr8.4:15189782-15189801 MS.gene036860:CDS 35.0%
AAGGTAAAATTAGACGGAGA+GGG - chr8.4:15189635-15189654 None:intergenic 35.0%
AGCATATGGTTATGAGTCTT+GGG + chr8.4:15189783-15189802 MS.gene036860:CDS 35.0%
ATTACTCAATTCAACTACCC+AGG + chr8.4:15189359-15189378 MS.gene036860:CDS 35.0%
CAATATCTCACTTCTTCACT+CGG + chr8.4:15189398-15189417 MS.gene036860:CDS 35.0%
CCAGTCAAAAACCAAAAAAC+AGG - chr8.4:15189863-15189882 None:intergenic 35.0%
TGTGAATAAGTTTCTGACTG+TGG + chr8.4:15190051-15190070 MS.gene036860:CDS 35.0%
TTAGCGATTGGAATAGTGAA+AGG - chr8.4:15189464-15189483 None:intergenic 35.0%
! TTCTTGGGCTTTTGCTTATT+TGG + chr8.4:15189730-15189749 MS.gene036860:CDS 35.0%
! TTTGACTGGTGAGAATTTTG+AGG + chr8.4:15189874-15189893 MS.gene036860:CDS 35.0%
!! GATGTTGAAAGTAGTCTTGT+TGG + chr8.4:15190220-15190239 MS.gene036860:CDS 35.0%
!!! CCTGTTTTTTGGTTTTTGAC+TGG + chr8.4:15189860-15189879 MS.gene036860:CDS 35.0%
!!! TGATGATTGCTCCTGTTTTT+TGG + chr8.4:15189849-15189868 MS.gene036860:CDS 35.0%
ACCAGCAAGAATAACAACCA+AGG - chr8.4:15189663-15189682 None:intergenic 40.0%
ATAAGCCGTGAGTGTAAAAC+CGG - chr8.4:15189711-15189730 None:intergenic 40.0%
CTTATTCACAACCCCAGTAA+CGG - chr8.4:15190041-15190060 None:intergenic 40.0%
GAAGATGAGAGTGAAGGAAA+GGG + chr8.4:15190256-15190275 MS.gene036860:CDS 40.0%
GGCGATTAATGTGACGATTT+GGG + chr8.4:15190072-15190091 MS.gene036860:CDS 40.0%
GGGAAGAATTTCTTAGCGAT+TGG - chr8.4:15189476-15189495 None:intergenic 40.0%
GGGTGTTCTTTATCAGCAAT+CGG + chr8.4:15190147-15190166 MS.gene036860:CDS 40.0%
GGTTACCATTACTACTGAGA+TGG + chr8.4:15189751-15189770 MS.gene036860:CDS 40.0%
GTAACGCAGTTAAAAGACCT+GGG - chr8.4:15189379-15189398 None:intergenic 40.0%
GTTTCGTTGTCTTGTGTGTT+TGG + chr8.4:15189956-15189975 MS.gene036860:CDS 40.0%
TACACTCACGGCTTATTCTT+GGG + chr8.4:15189715-15189734 MS.gene036860:CDS 40.0%
TGAAGATGAGAGTGAAGGAA+AGG + chr8.4:15190255-15190274 MS.gene036860:CDS 40.0%
TGGCGATTAATGTGACGATT+TGG + chr8.4:15190071-15190090 MS.gene036860:CDS 40.0%
TGTAACGCAGTTAAAAGACC+TGG - chr8.4:15189380-15189399 None:intergenic 40.0%
TTACACTCACGGCTTATTCT+TGG + chr8.4:15189714-15189733 MS.gene036860:CDS 40.0%
! GATTCTGAAGATGAGAGTGA+AGG + chr8.4:15190250-15190269 MS.gene036860:CDS 40.0%
! GTTCGATGTGAATGCGTTTT+TGG + chr8.4:15189931-15189950 MS.gene036860:CDS 40.0%
! TCCTTGGTTGTTATTCTTGC+TGG + chr8.4:15189659-15189678 MS.gene036860:CDS 40.0%
! TGATTCCGGTTTTACACTCA+CGG + chr8.4:15189703-15189722 MS.gene036860:CDS 40.0%
!! AGTCTTGGGTTGAATACTTG+GGG + chr8.4:15189797-15189816 MS.gene036860:CDS 40.0%
!! GAAAGTAGTCTTGTTGGTGA+TGG + chr8.4:15190226-15190245 MS.gene036860:CDS 40.0%
!! GAGTCTTGGGTTGAATACTT+GGG + chr8.4:15189796-15189815 MS.gene036860:CDS 40.0%
!! GGTTATGTTGCTACTGATTC+CGG + chr8.4:15189689-15189708 MS.gene036860:CDS 40.0%
!! TGAGTCTTGGGTTGAATACT+TGG + chr8.4:15189795-15189814 MS.gene036860:CDS 40.0%
!!! TCACTCGGTGTTTACCTTTT+AGG + chr8.4:15189413-15189432 MS.gene036860:CDS 40.0%
ACCGATTACTAAGCCACTTG+TGG - chr8.4:15189294-15189313 None:intergenic 45.0%
AGCAAACAAACCAAACCAGC+TGG - chr8.4:15190112-15190131 None:intergenic 45.0%
CTTTATCAGCAATCGGTGAC+TGG + chr8.4:15190154-15190173 MS.gene036860:CDS 45.0%
GCCACAAGTGGCTTAGTAAT+CGG + chr8.4:15189290-15189309 MS.gene036860:CDS 45.0%
TATCAACGTTAGCGTGACGA+AGG - chr8.4:15189544-15189563 None:intergenic 45.0%
! AAGAAGAAAACGAGTGCTGC+AGG - chr8.4:15189497-15189516 None:intergenic 45.0%
! AGAAGAAAACGAGTGCTGCA+GGG - chr8.4:15189496-15189515 None:intergenic 45.0%
!! CGATGTGAATGCGTTTTTGG+CGG + chr8.4:15189934-15189953 MS.gene036860:CDS 45.0%
!! GTTATTCTTGCTGGTGCTGT+TGG + chr8.4:15189668-15189687 MS.gene036860:CDS 45.0%
AACCCCAGTAACGGTAAACG+CGG - chr8.4:15190032-15190051 None:intergenic 50.0%
ACTCCCAGTACTCGATCTCA+AGG - chr8.4:15189909-15189928 None:intergenic 50.0%
ACTGCCTTGAGATCGAGTAC+TGG + chr8.4:15189902-15189921 MS.gene036860:CDS 50.0%
CAACAACACTACGCCACAAG+TGG + chr8.4:15189278-15189297 MS.gene036860:CDS 50.0%
CTACCGCGTTTACCGTTACT+GGG + chr8.4:15190026-15190045 MS.gene036860:CDS 50.0%
CTGCCTTGAGATCGAGTACT+GGG + chr8.4:15189903-15189922 MS.gene036860:CDS 50.0%
GATAAGCATGCTAGTCCAGC+TGG + chr8.4:15190094-15190113 MS.gene036860:CDS 50.0%
GTGACGAAGGAGATTGGTGT+TGG - chr8.4:15189531-15189550 None:intergenic 50.0%
GTTAGCGTGACGAAGGAGAT+TGG - chr8.4:15189537-15189556 None:intergenic 50.0%
TACCGCGTTTACCGTTACTG+GGG + chr8.4:15190027-15190046 MS.gene036860:CDS 50.0%
AGCGAGCGCAACAAGAAGAG+GGG - chr8.4:15189591-15189610 None:intergenic 55.0%
CAGCAATCGGTGACTGGAAG+TGG + chr8.4:15190160-15190179 MS.gene036860:CDS 55.0%
CAGCGAGCGCAACAAGAAGA+GGG - chr8.4:15189592-15189611 None:intergenic 55.0%
CTCGCTGATACTGCGTTTCG+CGG + chr8.4:15189605-15189624 MS.gene036860:CDS 55.0%
GACTGGAAGTGGTTCGCAAC+AGG + chr8.4:15190171-15190190 MS.gene036860:CDS 55.0%
GCATGCTAGTCCAGCTGGTT+TGG + chr8.4:15190099-15190118 MS.gene036860:CDS 55.0%
GCTACCGCGTTTACCGTTAC+TGG + chr8.4:15190025-15190044 MS.gene036860:CDS 55.0%
TCAGCGAGCGCAACAAGAAG+AGG - chr8.4:15189593-15189612 None:intergenic 55.0%
TGGAAGTGGTTCGCAACAGG+CGG + chr8.4:15190174-15190193 MS.gene036860:CDS 55.0%
TGGTTCGCAACAGGCGGTTG+TGG + chr8.4:15190180-15190199 MS.gene036860:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 15189257 15190297 15189257 ID=MS.gene036860
chr8.4 mRNA 15189257 15190297 15189257 ID=MS.gene036860.t1;Parent=MS.gene036860
chr8.4 exon 15189257 15190297 15189257 ID=MS.gene036860.t1.exon1;Parent=MS.gene036860.t1
chr8.4 CDS 15189257 15190297 15189257 ID=cds.MS.gene036860.t1;Parent=MS.gene036860.t1
Gene Sequence

>MS.gene036860

ATGTCATCAACAATATTGAAACAACAACACTACGCCACAAGTGGCTTAGTAATCGGTTACGCACTCTGTTCAAGCTTATTAGCAATCATAAACAAATACGCTATTACTCAATTCAACTACCCAGGTCTTTTAACTGCGTTACAATATCTCACTTCTTCACTCGGTGTTTACCTTTTAGGTAAATTAGGGTTTCTTCATCACGATCCTTTCACTATTCCAATCGCTAAGAAATTCTTCCCTGCAGCACTCGTTTTCTTCTTAGCGATCTTCACCAACACCAATCTCCTTCGTCACGCTAACGTTGATACGTTCATTGTTTTCAGATCTCTTACCCCTCTTCTTGTTGCGCTCGCTGATACTGCGTTTCGCGGTCAACCCTCTCCGTCTAATTTTACCTTTTTGTCCTTGGTTGTTATTCTTGCTGGTGCTGTTGGTTATGTTGCTACTGATTCCGGTTTTACACTCACGGCTTATTCTTGGGCTTTTGCTTATTTGGTTACCATTACTACTGAGATGGTTTATATTAAGCATATGGTTATGAGTCTTGGGTTGAATACTTGGGGTTTTGTGCTTTATAATAATGTATTGTCATTGATGATTGCTCCTGTTTTTTGGTTTTTGACTGGTGAGAATTTTGAGGTTTTTACTGCCTTGAGATCGAGTACTGGGAGTTTGTTCGATGTGAATGCGTTTTTGGCGGTTTCGTTGTCTTGTGTGTTTGGTTTGTTGATTAGTTTCTTTGGATTTGCTGCTAGAAAAGCTGTTTCTGCTACCGCGTTTACCGTTACTGGGGTTGTGAATAAGTTTCTGACTGTGGCGATTAATGTGACGATTTGGGATAAGCATGCTAGTCCAGCTGGTTTGGTTTGTTTGCTTTTTACGATTATTGGGGGTGTTCTTTATCAGCAATCGGTGACTGGAAGTGGTTCGCAACAGGCGGTTGTGGTGACTAAACAACAGTCTGATGTTGAAAGTAGTCTTGTTGGTGATGGTGATTCTGAAGATGAGAGTGAAGGAAAGGGTAAACATGCTTCTCTATGA

Protein sequence

>MS.gene036860.t1

MSSTILKQQHYATSGLVIGYALCSSLLAIINKYAITQFNYPGLLTALQYLTSSLGVYLLGKLGFLHHDPFTIPIAKKFFPAALVFFLAIFTNTNLLRHANVDTFIVFRSLTPLLVALADTAFRGQPSPSNFTFLSLVVILAGAVGYVATDSGFTLTAYSWAFAYLVTITTEMVYIKHMVMSLGLNTWGFVLYNNVLSLMIAPVFWFLTGENFEVFTALRSSTGSLFDVNAFLAVSLSCVFGLLISFFGFAARKAVSATAFTVTGVVNKFLTVAINVTIWDKHASPAGLVCLLFTIIGGVLYQQSVTGSGSQQAVVVTKQQSDVESSLVGDGDSEDESEGKGKHASL