AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene038701


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038701.t1 MTR_4g113270 98.266 346 5 1 1 346 1 345 0.0 676
MS.gene038701.t1 MTR_4g113270 98.235 340 5 1 1 340 1 339 0.0 665
MS.gene038701.t1 MTR_1g100860 47.788 339 158 4 15 340 41 373 5.73e-98 295
MS.gene038701.t1 MTR_4g018770 47.198 339 160 4 15 340 35 367 8.72e-98 294
MS.gene038701.t1 MTR_1g100860 46.739 276 130 4 77 340 5 275 4.57e-76 235
MS.gene038701.t1 MTR_4g018770 46.570 277 129 4 77 340 5 275 6.75e-76 235
MS.gene038701.t1 MTR_3g463360 24.242 297 206 6 19 304 108 396 9.01e-13 69.3
MS.gene038701.t1 MTR_3g109070 27.692 260 164 7 18 267 20 265 2.14e-12 67.4
MS.gene038701.t1 MTR_3g109070 27.692 260 164 7 18 267 20 265 3.48e-12 66.2
MS.gene038701.t1 MTR_3g096310 23.102 303 218 5 18 311 45 341 6.48e-12 66.2
MS.gene038701.t1 MTR_3g109070 28.218 202 126 5 18 210 20 211 2.80e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene038701.t1 AT5G19980 71.304 345 92 2 2 346 3 340 6.17e-169 474
MS.gene038701.t1 AT1G76340 48.615 325 161 3 15 337 37 357 9.10e-101 302
MS.gene038701.t1 AT4G31600 26.848 257 170 5 18 267 19 264 3.66e-13 69.7
MS.gene038701.t1 AT2G13650 22.896 297 212 5 18 304 38 327 8.51e-12 65.9
MS.gene038701.t1 AT2G13650 22.896 297 212 5 18 304 38 327 8.51e-12 65.9
MS.gene038701.t1 AT4G31600 27.136 199 132 3 18 210 19 210 1.26e-11 65.1

Find 65 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGGTTGTTATTCTTGC+TGG 0.233454 8.4:+15270974 MS.gene038701:CDS
GCTACCGCGTTTACCGTTAC+TGG 0.269983 8.4:+15271340 MS.gene038701:CDS
GGTTTGTTGATTAGTTTCTT+TGG 0.270396 8.4:+15271292 MS.gene038701:CDS
GAAACCCTAATTTACCTAAA+AGG 0.287655 8.4:-15270742 None:intergenic
TTACACTCACGGCTTATTCT+TGG 0.301434 8.4:+15271029 MS.gene038701:CDS
TGAGTCTTGGGTTGAATACT+TGG 0.340183 8.4:+15271110 MS.gene038701:CDS
AATAACAACCAAGGACAAAA+AGG 0.342091 8.4:-15270966 None:intergenic
TACACTCACGGCTTATTCTT+GGG 0.343245 8.4:+15271030 MS.gene038701:CDS
GGTTATGTTGCTACTGATTC+CGG 0.348332 8.4:+15271004 MS.gene038701:CDS
CTACCGCGTTTACCGTTACT+GGG 0.359242 8.4:+15271341 MS.gene038701:CDS
TGGCAATTAATGTGACGATT+TGG 0.360897 8.4:+15271386 MS.gene038701:CDS
GTTTCGTTGTCTTGTGTGTT+TGG 0.368137 8.4:+15271271 MS.gene038701:CDS
GATGGTTTATATTAAGCATA+TGG 0.374607 8.4:+15271084 MS.gene038701:CDS
GCATGCTAGTCCAGCTGGTT+TGG 0.378044 8.4:+15271414 MS.gene038701:CDS
CAGCAATCGGTGACTGGAAA+TGG 0.381275 8.4:+15271475 MS.gene038701:CDS
AAGCATATGGTTATGAGTCT+TGG 0.383947 8.4:+15271097 MS.gene038701:CDS
AGCATATGGTTATGAGTCTT+GGG 0.397212 8.4:+15271098 MS.gene038701:CDS
GGCAATTAATGTGACGATTT+GGG 0.402674 8.4:+15271387 MS.gene038701:CDS
GTTATTCTTGCTGGTGCTGT+TGG 0.419854 8.4:+15270983 MS.gene038701:CDS
GACTGGAAATGGTTCGCAAC+AGG 0.428349 8.4:+15271486 MS.gene038701:CDS
GATGTTGAAAGTAGTCTTGT+TGG 0.433983 8.4:+15271535 MS.gene038701:CDS
GCCACAAGTGGCTTAGTAAT+CGG 0.437849 8.4:+15270605 MS.gene038701:CDS
GGGAAGAATTTCTTAGCGAT+TGG 0.443284 8.4:-15270788 None:intergenic
AAGAAGAAAACGAGTGCTGC+AGG 0.450306 8.4:-15270809 None:intergenic
GAGTCTTGGGTTGAATACTT+GGG 0.451840 8.4:+15271111 MS.gene038701:CDS
CTTTATCAGCAATCGGTGAC+TGG 0.470901 8.4:+15271469 MS.gene038701:CDS
GGGTGTTCTTTATCAGCAAT+CGG 0.471127 8.4:+15271462 MS.gene038701:CDS
TGGTTCGCAACAGGCGGTTG+TGG 0.475038 8.4:+15271495 MS.gene038701:CDS
GTTAGCGTGACGAAGGAGAT+TGG 0.477146 8.4:-15270849 None:intergenic
TGAAGAAGTGAGATATTGTA+AGG 0.495489 8.4:-15270708 None:intergenic
ATAAGCCGTGAGTGTAAAAC+CGG 0.495535 8.4:-15271023 None:intergenic
ACTGCCTTGAGATCGAGTAC+TGG 0.496102 8.4:+15271217 MS.gene038701:CDS
TGTAAGGCTGTTAAAAGACC+TGG 0.499578 8.4:-15270692 None:intergenic
CAATATCTCACTTCTTCACT+CGG 0.505402 8.4:+15270713 MS.gene038701:CDS
CTGCCTTGAGATCGAGTACT+GGG 0.507997 8.4:+15271218 MS.gene038701:CDS
AGTCTTGGGTTGAATACTTG+GGG 0.509311 8.4:+15271112 MS.gene038701:CDS
GATAAGCATGCTAGTCCAGC+TGG 0.512847 8.4:+15271409 MS.gene038701:CDS
TGAAGATGAGAGTGAAGGAA+AGG 0.515215 8.4:+15271570 MS.gene038701:CDS
CTCGCTGATACTGCGTTTCG+CGG 0.516331 8.4:+15270920 MS.gene038701:CDS
ATAAACCATCTCAGTAGTAA+TGG 0.529935 8.4:-15271071 None:intergenic
GAAGATGAGAGTGAAGGAAA+GGG 0.531374 8.4:+15271571 MS.gene038701:CDS
AGCAAACAAACCAAACCAGC+TGG 0.537823 8.4:-15271424 None:intergenic
TTAGCGATTGGAATAGTGAA+AGG 0.542673 8.4:-15270776 None:intergenic
GGTTACCATTACTACTGAGA+TGG 0.551400 8.4:+15271066 MS.gene038701:CDS
CAGCGAGAGCAACAAGAAGA+GGG 0.556974 8.4:-15270904 None:intergenic
TCAGCGAGAGCAACAAGAAG+AGG 0.569697 8.4:-15270905 None:intergenic
CAACAACACTACGCCACAAG+TGG 0.587960 8.4:+15270593 MS.gene038701:CDS
GAAAGTAGTCTTGTTGGTGA+TGG 0.589182 8.4:+15271541 MS.gene038701:CDS
ACCAGCAAGAATAACAACCA+AGG 0.595444 8.4:-15270975 None:intergenic
AAGGTAAAATTAGACGGAGA+GGG 0.604146 8.4:-15270947 None:intergenic
CTTATTCACAACCCCAGTAA+CGG 0.607538 8.4:-15271353 None:intergenic
GTAAGGCTGTTAAAAGACCT+GGG 0.614797 8.4:-15270691 None:intergenic
AAAGGTAAAATTAGACGGAG+AGG 0.624222 8.4:-15270948 None:intergenic
GTGACGAAGGAGATTGGTGT+TGG 0.632002 8.4:-15270843 None:intergenic
ACTCCCAGTACTCGATCTCA+AGG 0.635928 8.4:-15271221 None:intergenic
ACCGATTACTAAGCCACTTG+TGG 0.637733 8.4:-15270606 None:intergenic
TATCAACGTTAGCGTGACGA+AGG 0.639770 8.4:-15270856 None:intergenic
TGGAAATGGTTCGCAACAGG+CGG 0.646327 8.4:+15271489 MS.gene038701:CDS
ATTACTCAATTCAACTACCC+AGG 0.649152 8.4:+15270674 MS.gene038701:CDS
AGAAGAAAACGAGTGCTGCA+GGG 0.656660 8.4:-15270808 None:intergenic
AGCGAGAGCAACAAGAAGAG+GGG 0.662395 8.4:-15270903 None:intergenic
TGTGAATAAGTTTCTGACTG+TGG 0.688581 8.4:+15271366 MS.gene038701:CDS
GAATCTGAAGATGAGAGTGA+AGG 0.699145 8.4:+15271565 MS.gene038701:CDS
TACCGCGTTTACCGTTACTG+GGG 0.705827 8.4:+15271342 MS.gene038701:CDS
AACCCCAGTAACGGTAAACG+CGG 0.739549 8.4:-15271344 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACCTTTTAGGTAAATTA+GGG + chr8.4:15270738-15270757 MS.gene038701:CDS 20.0%
! GACAAAAAGGTAAAATTAGA+CGG - chr8.4:15270956-15270975 None:intergenic 25.0%
! GATGGTTTATATTAAGCATA+TGG + chr8.4:15271084-15271103 MS.gene038701:CDS 25.0%
!! TAATTTTACCTTTTTGTCCT+TGG + chr8.4:15270958-15270977 MS.gene038701:CDS 25.0%
!!! GTTTACCTTTTAGGTAAATT+AGG + chr8.4:15270737-15270756 MS.gene038701:CDS 25.0%
!!! GTTTGCTTTTTACGATTATT+GGG + chr8.4:15271440-15271459 MS.gene038701:CDS 25.0%
!!! TGTTTGCTTTTTACGATTAT+TGG + chr8.4:15271439-15271458 MS.gene038701:CDS 25.0%
!!! TTTGCTTTTTACGATTATTG+GGG + chr8.4:15271441-15271460 MS.gene038701:CDS 25.0%
AATAACAACCAAGGACAAAA+AGG - chr8.4:15270969-15270988 None:intergenic 30.0%
ATAAACCATCTCAGTAGTAA+TGG - chr8.4:15271074-15271093 None:intergenic 30.0%
GAAACCCTAATTTACCTAAA+AGG - chr8.4:15270745-15270764 None:intergenic 30.0%
TGAAGAAGTGAGATATTGTA+AGG - chr8.4:15270711-15270730 None:intergenic 30.0%
!! GGTTTGTTGATTAGTTTCTT+TGG + chr8.4:15271292-15271311 MS.gene038701:CDS 30.0%
!!! TTGCTTTTTACGATTATTGG+GGG + chr8.4:15271442-15271461 MS.gene038701:CDS 30.0%
AAAGGTAAAATTAGACGGAG+AGG - chr8.4:15270951-15270970 None:intergenic 35.0%
AAGCATATGGTTATGAGTCT+TGG + chr8.4:15271097-15271116 MS.gene038701:CDS 35.0%
AAGGTAAAATTAGACGGAGA+GGG - chr8.4:15270950-15270969 None:intergenic 35.0%
AGCATATGGTTATGAGTCTT+GGG + chr8.4:15271098-15271117 MS.gene038701:CDS 35.0%
ATTACTCAATTCAACTACCC+AGG + chr8.4:15270674-15270693 MS.gene038701:CDS 35.0%
CAATATCTCACTTCTTCACT+CGG + chr8.4:15270713-15270732 MS.gene038701:CDS 35.0%
CCAGTCAAAAACCAAAAAAC+AGG - chr8.4:15271178-15271197 None:intergenic 35.0%
GGCAATTAATGTGACGATTT+GGG + chr8.4:15271387-15271406 MS.gene038701:CDS 35.0%
TGGCAATTAATGTGACGATT+TGG + chr8.4:15271386-15271405 MS.gene038701:CDS 35.0%
TGTGAATAAGTTTCTGACTG+TGG + chr8.4:15271366-15271385 MS.gene038701:CDS 35.0%
TTAGCGATTGGAATAGTGAA+AGG - chr8.4:15270779-15270798 None:intergenic 35.0%
! TTCTTGGGCTTTTGCTTATT+TGG + chr8.4:15271045-15271064 MS.gene038701:CDS 35.0%
! TTTGACTGGTGAGAATTTTG+AGG + chr8.4:15271189-15271208 MS.gene038701:CDS 35.0%
!! GATGTTGAAAGTAGTCTTGT+TGG + chr8.4:15271535-15271554 MS.gene038701:CDS 35.0%
!!! CCTGTTTTTTGGTTTTTGAC+TGG + chr8.4:15271175-15271194 MS.gene038701:CDS 35.0%
!!! TGATGATTGCTCCTGTTTTT+TGG + chr8.4:15271164-15271183 MS.gene038701:CDS 35.0%
ACCAGCAAGAATAACAACCA+AGG - chr8.4:15270978-15270997 None:intergenic 40.0%
ATAAGCCGTGAGTGTAAAAC+CGG - chr8.4:15271026-15271045 None:intergenic 40.0%
CTTATTCACAACCCCAGTAA+CGG - chr8.4:15271356-15271375 None:intergenic 40.0%
GAAGATGAGAGTGAAGGAAA+GGG + chr8.4:15271571-15271590 MS.gene038701:CDS 40.0%
GAATCTGAAGATGAGAGTGA+AGG + chr8.4:15271565-15271584 MS.gene038701:CDS 40.0%
GGGAAGAATTTCTTAGCGAT+TGG - chr8.4:15270791-15270810 None:intergenic 40.0%
GGGTGTTCTTTATCAGCAAT+CGG + chr8.4:15271462-15271481 MS.gene038701:CDS 40.0%
GGTTACCATTACTACTGAGA+TGG + chr8.4:15271066-15271085 MS.gene038701:CDS 40.0%
GTAAGGCTGTTAAAAGACCT+GGG - chr8.4:15270694-15270713 None:intergenic 40.0%
GTTTCGTTGTCTTGTGTGTT+TGG + chr8.4:15271271-15271290 MS.gene038701:CDS 40.0%
TACACTCACGGCTTATTCTT+GGG + chr8.4:15271030-15271049 MS.gene038701:CDS 40.0%
TGAAGATGAGAGTGAAGGAA+AGG + chr8.4:15271570-15271589 MS.gene038701:CDS 40.0%
TGTAAGGCTGTTAAAAGACC+TGG - chr8.4:15270695-15270714 None:intergenic 40.0%
TTACACTCACGGCTTATTCT+TGG + chr8.4:15271029-15271048 MS.gene038701:CDS 40.0%
! GTTCGATGTGAATGCGTTTT+TGG + chr8.4:15271246-15271265 MS.gene038701:CDS 40.0%
! TCCTTGGTTGTTATTCTTGC+TGG + chr8.4:15270974-15270993 MS.gene038701:CDS 40.0%
! TGATTCCGGTTTTACACTCA+CGG + chr8.4:15271018-15271037 MS.gene038701:CDS 40.0%
!! AGTCTTGGGTTGAATACTTG+GGG + chr8.4:15271112-15271131 MS.gene038701:CDS 40.0%
!! GAAAGTAGTCTTGTTGGTGA+TGG + chr8.4:15271541-15271560 MS.gene038701:CDS 40.0%
!! GAGTCTTGGGTTGAATACTT+GGG + chr8.4:15271111-15271130 MS.gene038701:CDS 40.0%
!! GGTTATGTTGCTACTGATTC+CGG + chr8.4:15271004-15271023 MS.gene038701:CDS 40.0%
!! TGAGTCTTGGGTTGAATACT+TGG + chr8.4:15271110-15271129 MS.gene038701:CDS 40.0%
!!! TCACTCGGTGTTTACCTTTT+AGG + chr8.4:15270728-15270747 MS.gene038701:CDS 40.0%
ACCGATTACTAAGCCACTTG+TGG - chr8.4:15270609-15270628 None:intergenic 45.0%
AGCAAACAAACCAAACCAGC+TGG - chr8.4:15271427-15271446 None:intergenic 45.0%
CTTTATCAGCAATCGGTGAC+TGG + chr8.4:15271469-15271488 MS.gene038701:CDS 45.0%
GCCACAAGTGGCTTAGTAAT+CGG + chr8.4:15270605-15270624 MS.gene038701:CDS 45.0%
TATCAACGTTAGCGTGACGA+AGG - chr8.4:15270859-15270878 None:intergenic 45.0%
! AAGAAGAAAACGAGTGCTGC+AGG - chr8.4:15270812-15270831 None:intergenic 45.0%
! AGAAGAAAACGAGTGCTGCA+GGG - chr8.4:15270811-15270830 None:intergenic 45.0%
!! CGATGTGAATGCGTTTTTGG+CGG + chr8.4:15271249-15271268 MS.gene038701:CDS 45.0%
!! GTTATTCTTGCTGGTGCTGT+TGG + chr8.4:15270983-15271002 MS.gene038701:CDS 45.0%
AACCCCAGTAACGGTAAACG+CGG - chr8.4:15271347-15271366 None:intergenic 50.0%
ACTCCCAGTACTCGATCTCA+AGG - chr8.4:15271224-15271243 None:intergenic 50.0%
ACTGCCTTGAGATCGAGTAC+TGG + chr8.4:15271217-15271236 MS.gene038701:CDS 50.0%
AGCGAGAGCAACAAGAAGAG+GGG - chr8.4:15270906-15270925 None:intergenic 50.0%
CAACAACACTACGCCACAAG+TGG + chr8.4:15270593-15270612 MS.gene038701:CDS 50.0%
CAGCAATCGGTGACTGGAAA+TGG + chr8.4:15271475-15271494 MS.gene038701:CDS 50.0%
CAGCGAGAGCAACAAGAAGA+GGG - chr8.4:15270907-15270926 None:intergenic 50.0%
CTACCGCGTTTACCGTTACT+GGG + chr8.4:15271341-15271360 MS.gene038701:CDS 50.0%
CTGCCTTGAGATCGAGTACT+GGG + chr8.4:15271218-15271237 MS.gene038701:CDS 50.0%
GACTGGAAATGGTTCGCAAC+AGG + chr8.4:15271486-15271505 MS.gene038701:CDS 50.0%
GATAAGCATGCTAGTCCAGC+TGG + chr8.4:15271409-15271428 MS.gene038701:CDS 50.0%
GTGACGAAGGAGATTGGTGT+TGG - chr8.4:15270846-15270865 None:intergenic 50.0%
GTTAGCGTGACGAAGGAGAT+TGG - chr8.4:15270852-15270871 None:intergenic 50.0%
TACCGCGTTTACCGTTACTG+GGG + chr8.4:15271342-15271361 MS.gene038701:CDS 50.0%
TCAGCGAGAGCAACAAGAAG+AGG - chr8.4:15270908-15270927 None:intergenic 50.0%
TGGAAATGGTTCGCAACAGG+CGG + chr8.4:15271489-15271508 MS.gene038701:CDS 50.0%
CTCGCTGATACTGCGTTTCG+CGG + chr8.4:15270920-15270939 MS.gene038701:CDS 55.0%
GCATGCTAGTCCAGCTGGTT+TGG + chr8.4:15271414-15271433 MS.gene038701:CDS 55.0%
GCTACCGCGTTTACCGTTAC+TGG + chr8.4:15271340-15271359 MS.gene038701:CDS 55.0%
TGGTTCGCAACAGGCGGTTG+TGG + chr8.4:15271495-15271514 MS.gene038701:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 15270572 15271612 15270572 ID=MS.gene038701
chr8.4 mRNA 15270572 15271612 15270572 ID=MS.gene038701.t1;Parent=MS.gene038701
chr8.4 exon 15270572 15271612 15270572 ID=MS.gene038701.t1.exon1;Parent=MS.gene038701.t1
chr8.4 CDS 15270572 15271612 15270572 ID=cds.MS.gene038701.t1;Parent=MS.gene038701.t1
Gene Sequence

>MS.gene038701

ATGTCATCAACAATATTGAAACAACAACACTACGCCACAAGTGGCTTAGTAATCGGTTACGCACTCTGTTCAAGCTTATTAGCAATCATAAACAAATACGCTATTACTCAATTCAACTACCCAGGTCTTTTAACAGCCTTACAATATCTCACTTCTTCACTCGGTGTTTACCTTTTAGGTAAATTAGGGTTTCTTCATCACGATCCTTTCACTATTCCAATCGCTAAGAAATTCTTCCCTGCAGCACTCGTTTTCTTCTTAGCGATCTTCACCAACACCAATCTCCTTCGTCACGCTAACGTTGATACGTTCATTGTTTTCAGATCTCTTACCCCTCTTCTTGTTGCTCTCGCTGATACTGCGTTTCGCGGTCAACCCTCTCCGTCTAATTTTACCTTTTTGTCCTTGGTTGTTATTCTTGCTGGTGCTGTTGGTTATGTTGCTACTGATTCCGGTTTTACACTCACGGCTTATTCTTGGGCTTTTGCTTATTTGGTTACCATTACTACTGAGATGGTTTATATTAAGCATATGGTTATGAGTCTTGGGTTGAATACTTGGGGTTTTGTGCTTTATAATAATGTATTGTCATTGATGATTGCTCCTGTTTTTTGGTTTTTGACTGGTGAGAATTTTGAGGTTTTTACTGCCTTGAGATCGAGTACTGGGAGTTTGTTCGATGTGAATGCGTTTTTGGCGGTTTCGTTGTCTTGTGTGTTTGGTTTGTTGATTAGTTTCTTTGGATTTGCTGCTAGAAAAGCTGTTTCTGCTACCGCGTTTACCGTTACTGGGGTTGTGAATAAGTTTCTGACTGTGGCAATTAATGTGACGATTTGGGATAAGCATGCTAGTCCAGCTGGTTTGGTTTGTTTGCTTTTTACGATTATTGGGGGTGTTCTTTATCAGCAATCGGTGACTGGAAATGGTTCGCAACAGGCGGTTGTGGTGACTAAACAACAGTCTGATGTTGAAAGTAGTCTTGTTGGTGATGGTGAATCTGAAGATGAGAGTGAAGGAAAGGGTAAACATGCTTCTCTATGA

Protein sequence

>MS.gene038701.t1

MSSTILKQQHYATSGLVIGYALCSSLLAIINKYAITQFNYPGLLTALQYLTSSLGVYLLGKLGFLHHDPFTIPIAKKFFPAALVFFLAIFTNTNLLRHANVDTFIVFRSLTPLLVALADTAFRGQPSPSNFTFLSLVVILAGAVGYVATDSGFTLTAYSWAFAYLVTITTEMVYIKHMVMSLGLNTWGFVLYNNVLSLMIAPVFWFLTGENFEVFTALRSSTGSLFDVNAFLAVSLSCVFGLLISFFGFAARKAVSATAFTVTGVVNKFLTVAINVTIWDKHASPAGLVCLLFTIIGGVLYQQSVTGNGSQQAVVVTKQQSDVESSLVGDGESEDESEGKGKHASL