Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041828.t1 | XP_024638642.1 | 100 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 9.30E-63 | 250.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041828.t1 | Q9CX48 | 55.9 | 34 | 15 | 0 | 86 | 119 | 20 | 53 | 6.9e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041828.t1 | A0A396IFI3 | 100.0 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 6.7e-63 | 250.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045256 | MS.gene041828 | PPI |
| MS.gene75592 | MS.gene041828 | PPI |
| MS.gene041828 | MS.gene066824 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041828.t1 | MTR_1g032030 | 89.552 | 67 | 6 | 1 | 18 | 83 | 15 | 81 | 3.47e-22 | 90.1 |
| MS.gene041828.t1 | MTR_1g032370 | 89.552 | 67 | 6 | 1 | 18 | 83 | 15 | 81 | 3.47e-22 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041828.t1 | AT5G49400 | 73.944 | 142 | 22 | 3 | 1 | 129 | 1 | 140 | 3.13e-53 | 174 |
Find 0 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTTAAAAACCCTAAATTG+AGG | - | 32443:9943-9962 | MS.gene041828:CDS | 25.0% |
| !! | ATCTGATTATTCTTCTAGTT+CGG | - | 32443:10148-10167 | MS.gene041828:CDS | 25.0% |
| ACTGAGAAAGCTGTTAAATT+GGG | - | 32443:9783-9802 | MS.gene041828:CDS | 30.0% | |
| TAATAATCATCGTGATGTGA+AGG | - | 32443:10010-10029 | MS.gene041828:CDS | 30.0% | |
| TACTGAGAAAGCTGTTAAAT+TGG | - | 32443:9782-9801 | MS.gene041828:CDS | 30.0% | |
| TAGTAAGAAGGAAGAGATTA+AGG | - | 32443:9602-9621 | MS.gene041828:CDS | 30.0% | |
| !! | TCTGATTATTCTTCTAGTTC+GGG | - | 32443:10149-10168 | MS.gene041828:CDS | 30.0% |
| AAGCTGTTAAATTGGGTGAA+AGG | - | 32443:9790-9809 | MS.gene041828:CDS | 35.0% | |
| ATTCTGATGATAAAAGCAGC+AGG | - | 32443:10324-10343 | MS.gene041828:CDS | 35.0% | |
| ATTTCAGTGTAAGAATGAGC+GGG | - | 32443:9890-9909 | MS.gene041828:CDS | 35.0% | |
| CATTTCAGTGTAAGAATGAG+CGG | - | 32443:9889-9908 | MS.gene041828:CDS | 35.0% | |
| GAATCATCTGATTCAGATTC+TGG | - | 32443:10290-10309 | MS.gene041828:CDS | 35.0% | |
| TGATTCAGGTAGTAATAGTG+AGG | - | 32443:10076-10095 | MS.gene041828:CDS | 35.0% | |
| TGTTAAATTGGGTGAAAGGA+AGG | - | 32443:9794-9813 | MS.gene041828:CDS | 35.0% | |
| TTTATATTTCAAGGCCATCG+AGG | - | 32443:9913-9932 | MS.gene041828:CDS | 35.0% | |
| ! | ATGAGCGGGTTTATATTTCA+AGG | - | 32443:9904-9923 | MS.gene041828:CDS | 35.0% |
| ACACATCTGGCCTCAATTTA+GGG | + | 32443:9956-9975 | None:intergenic | 40.0% | |
| ACCTGAATCATACTTCCTCT+TGG | + | 32443:10066-10085 | None:intergenic | 40.0% | |
| AGAAGAATAATCAGATCCCG+TGG | + | 32443:10144-10163 | None:intergenic | 40.0% | |
| AGAAGAGCAAAGGAAAAAGC+AGG | - | 32443:10249-10268 | MS.gene041828:CDS | 40.0% | |
| CAGACTAAGAAGAAGAGCAA+AGG | - | 32443:10239-10258 | MS.gene041828:CDS | 40.0% | |
| TAATCATCGTGATGTGAAGG+AGG | - | 32443:10013-10032 | MS.gene041828:CDS | 40.0% | |
| TCTTTCTTCCTCTGATTCAG+AGG | + | 32443:10186-10205 | None:intergenic | 40.0% | |
| TGAGTTCGAAAAAGTCCAAG+AGG | - | 32443:10048-10067 | MS.gene041828:CDS | 40.0% | |
| ! | TCCAAGAGGAAGTATGATTC+AGG | - | 32443:10062-10081 | MS.gene041828:CDS | 40.0% |
| ! | TGTGTCTGTGGGTTATGATT+TGG | - | 32443:9971-9990 | MS.gene041828:CDS | 40.0% |
| !!! | GGACTTTTTCGAACTCACTT+TGG | + | 32443:10045-10064 | None:intergenic | 40.0% |
| AAAGCAGCAGGAGAAAGAAG+AGG | - | 32443:10336-10355 | MS.gene041828:CDS | 45.0% | |
| AGAGCAAAGGAAAAAGCAGG+AGG | - | 32443:10252-10271 | MS.gene041828:CDS | 45.0% | |
| CAGCATTAACTGCTGCCAAA+GGG | - | 32443:9628-9647 | MS.gene041828:CDS | 45.0% | |
| CGTATGGTCAGAAGGAAGAA+GGG | - | 32443:9706-9725 | MS.gene041828:CDS | 45.0% | |
| GACACATCTGGCCTCAATTT+AGG | + | 32443:9957-9976 | None:intergenic | 45.0% | |
| TAGCAGATGGCAGGAGAAAA+GGG | - | 32443:9731-9750 | MS.gene041828:CDS | 45.0% | |
| TCATAACCCACAGACACATC+TGG | + | 32443:9969-9988 | None:intergenic | 45.0% | |
| TCGTGATGTGAAGGAGGAAA+AGG | - | 32443:10019-10038 | MS.gene041828:CDS | 45.0% | |
| TGTTAACGCGTATGGTCAGA+AGG | - | 32443:9698-9717 | MS.gene041828:CDS | 45.0% | |
| ! | GGAGAAAGAAGAGGCATAGT+CGG | - | 32443:10345-10364 | MS.gene041828:CDS | 45.0% |
| ! | TCAGTGTTTGAGACCGATAG+TGG | - | 32443:10101-10120 | MS.gene041828:CDS | 45.0% |
| ! | TTAAGTTGCTGAGTCCTCGA+TGG | + | 32443:9930-9949 | None:intergenic | 45.0% |
| !! | CAGTGTCAGAAGTGTTACCA+GGG | - | 32443:9855-9874 | MS.gene041828:CDS | 45.0% |
| ATTGAGGCCAGATGTGTCTG+TGG | - | 32443:9959-9978 | MS.gene041828:CDS | 50.0% | |
| CAGCTCATCCTCTGAATCAG+AGG | - | 32443:10175-10194 | MS.gene041828:CDS | 50.0% | |
| CCATCGACAGTCGACATTGT+AGG | + | 32443:9828-9847 | None:intergenic | 50.0% | |
| CGATGATCCACTGCCACTAT+CGG | + | 32443:10117-10136 | None:intergenic | 50.0% | |
| CTAGCAGATGGCAGGAGAAA+AGG | - | 32443:9730-9749 | MS.gene041828:CDS | 50.0% | |
| CTCTGATTCAGAGGATGAGC+TGG | + | 32443:10177-10196 | None:intergenic | 50.0% | |
| GCAGCATTAACTGCTGCCAA+AGG | - | 32443:9627-9646 | MS.gene041828:CDS | 50.0% | |
| GCGCGAAATGTTAACGCGTA+TGG | - | 32443:9690-9709 | MS.gene041828:CDS | 50.0% | |
| GCGTATGGTCAGAAGGAAGA+AGG | - | 32443:9705-9724 | MS.gene041828:CDS | 50.0% | |
| TTGAGCACGACTAAGCCCTT+TGG | + | 32443:9646-9665 | None:intergenic | 50.0% | |
| TTGAGGCCAGATGTGTCTGT+GGG | - | 32443:9960-9979 | MS.gene041828:CDS | 50.0% | |
| ! | CCTACAATGTCGACTGTCGA+TGG | - | 32443:9825-9844 | MS.gene041828:CDS | 50.0% |
| ! | CTTTTCTCCTGCCATCTGCT+AGG | + | 32443:9732-9751 | None:intergenic | 50.0% |
| ! | TTTGAGACCGATAGTGGCAG+TGG | - | 32443:10107-10126 | MS.gene041828:CDS | 50.0% |
| !! | GCAGTGTCAGAAGTGTTACC+AGG | - | 32443:9854-9873 | MS.gene041828:CDS | 50.0% |
| !! | GTCAGAAGTGTTACCAGGGT+GGG | - | 32443:9859-9878 | MS.gene041828:CDS | 50.0% |
| !! | TGTCAGAAGTGTTACCAGGG+TGG | - | 32443:9858-9877 | MS.gene041828:CDS | 50.0% |
| AGCCGAAAGAGCAGCAACTG+CGG | - | 32443:9665-9684 | MS.gene041828:CDS | 55.0% | |
| AGCCGCAGTTGCTGCTCTTT+CGG | + | 32443:9670-9689 | None:intergenic | 55.0% | |
| AGGAAGAAGGGCCTAGCAGA+TGG | - | 32443:9718-9737 | MS.gene041828:CDS | 55.0% | |
| AGGAGAAAAGGGAGGCGAAG+AGG | - | 32443:9742-9761 | MS.gene041828:CDS | 55.0% | |
| AGTGGATCATCGTCTGCCAC+GGG | - | 32443:10125-10144 | MS.gene041828:CDS | 55.0% | |
| CAGATGGCAGGAGAAAAGGG+AGG | - | 32443:9734-9753 | MS.gene041828:CDS | 55.0% | |
| CAGTGGATCATCGTCTGCCA+CGG | - | 32443:10124-10143 | MS.gene041828:CDS | 55.0% | |
| AGAAGGGCCTAGCAGATGGC+AGG | - | 32443:9722-9741 | MS.gene041828:CDS | 60.0% | |
| AGTGTTACCAGGGTGGGCAC+TGG | - | 32443:9865-9884 | MS.gene041828:CDS | 60.0% | |
| GAAATGTCCAGTGCCCACCC+TGG | + | 32443:9875-9894 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32443 | gene | 9594 | 10376 | 9594 | ID=MS.gene041828 |
| 32443 | mRNA | 9594 | 10376 | 9594 | ID=MS.gene041828.t1;Parent=MS.gene041828 |
| 32443 | exon | 9594 | 10376 | 9594 | ID=MS.gene041828.t1.exon1;Parent=MS.gene041828.t1 |
| 32443 | CDS | 9594 | 10376 | 9594 | ID=cds.MS.gene041828.t1;Parent=MS.gene041828.t1 |
| Gene Sequence |
| Protein sequence |
