Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042304.t1 | XP_024638642.1 | 100 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 9.30E-63 | 250.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042304.t1 | Q9CX48 | 55.9 | 34 | 15 | 0 | 86 | 119 | 20 | 53 | 6.9e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042304.t1 | A0A396IFI3 | 100.0 | 130 | 0 | 0 | 1 | 130 | 1 | 130 | 6.7e-63 | 250.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045256 | MS.gene042304 | PPI |
| MS.gene75592 | MS.gene042304 | PPI |
| MS.gene042304 | MS.gene066824 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042304.t1 | MTR_1g032030 | 89.552 | 67 | 6 | 1 | 18 | 83 | 15 | 81 | 3.40e-22 | 90.1 |
| MS.gene042304.t1 | MTR_1g032370 | 89.552 | 67 | 6 | 1 | 18 | 83 | 15 | 81 | 3.40e-22 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene042304.t1 | AT5G49400 | 73.944 | 142 | 22 | 3 | 1 | 129 | 1 | 140 | 2.61e-53 | 174 |
Find 63 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACATCTGGCCTCAATTT+AGG | 0.169557 | 8.3:+7122979 | None:intergenic |
| GAATCATCTGATTCAGATTC+TGG | 0.203322 | 8.3:-7122646 | MS.gene042304:CDS |
| ATCTGATTATTCTTCTAGTT+CGG | 0.261244 | 8.3:-7122788 | MS.gene042304:CDS |
| ACACATCTGGCCTCAATTTA+GGG | 0.271076 | 8.3:+7122980 | None:intergenic |
| AGCCGCAGTTGCTGCTCTTT+CGG | 0.288446 | 8.3:+7123266 | None:intergenic |
| TACTGAGAAAGCTGTTAAAT+TGG | 0.291730 | 8.3:-7123151 | MS.gene042304:CDS |
| TAGTAAGAAGGAAGAGATTA+AGG | 0.323676 | 8.3:-7123331 | MS.gene042304:CDS |
| TCTGATTATTCTTCTAGTTC+GGG | 0.339870 | 8.3:-7122787 | MS.gene042304:CDS |
| TCCAAGAGGAAGTATGATTC+AGG | 0.361886 | 8.3:-7122874 | MS.gene042304:CDS |
| CCATCGACAGTCGACATTGT+AGG | 0.383189 | 8.3:+7123108 | None:intergenic |
| TGTGTCTGTGGGTTATGATT+TGG | 0.415795 | 8.3:-7122962 | MS.gene042304:CDS |
| CTAGCAGATGGCAGGAGAAA+AGG | 0.416419 | 8.3:-7123203 | MS.gene042304:CDS |
| CTCTGATTCAGAGGATGAGC+TGG | 0.446364 | 8.3:+7122762 | None:intergenic |
| CAGCATTAACTGCTGCCAAA+GGG | 0.453514 | 8.3:-7123305 | MS.gene042304:CDS |
| TAGCAGATGGCAGGAGAAAA+GGG | 0.460886 | 8.3:-7123202 | MS.gene042304:CDS |
| ACTGAGAAAGCTGTTAAATT+GGG | 0.471361 | 8.3:-7123150 | MS.gene042304:CDS |
| ATGAGCGGGTTTATATTTCA+AGG | 0.478841 | 8.3:-7123029 | MS.gene042304:CDS |
| GCAGCATTAACTGCTGCCAA+AGG | 0.478872 | 8.3:-7123306 | MS.gene042304:CDS |
| TCTTTCTTCCTCTGATTCAG+AGG | 0.483023 | 8.3:+7122753 | None:intergenic |
| TCAGTGTTTGAGACCGATAG+TGG | 0.483942 | 8.3:-7122835 | MS.gene042304:CDS |
| ACCTGAATCATACTTCCTCT+TGG | 0.488586 | 8.3:+7122873 | None:intergenic |
| TCATAACCCACAGACACATC+TGG | 0.498000 | 8.3:+7122967 | None:intergenic |
| TGTTAAATTGGGTGAAAGGA+AGG | 0.511428 | 8.3:-7123139 | MS.gene042304:CDS |
| AGTGTTACCAGGGTGGGCAC+TGG | 0.514214 | 8.3:-7123068 | MS.gene042304:CDS |
| TCGTGATGTGAAGGAGGAAA+AGG | 0.516783 | 8.3:-7122917 | MS.gene042304:CDS |
| TTGAGCACGACTAAGCCCTT+TGG | 0.517177 | 8.3:+7123290 | None:intergenic |
| CAGTGGATCATCGTCTGCCA+CGG | 0.518690 | 8.3:-7122812 | MS.gene042304:CDS |
| GCAGTGTCAGAAGTGTTACC+AGG | 0.520961 | 8.3:-7123079 | MS.gene042304:CDS |
| GCGTATGGTCAGAAGGAAGA+AGG | 0.528077 | 8.3:-7123228 | MS.gene042304:CDS |
| CGATGATCCACTGCCACTAT+CGG | 0.530022 | 8.3:+7122822 | None:intergenic |
| GCGCGAAATGTTAACGCGTA+TGG | 0.534367 | 8.3:-7123243 | MS.gene042304:CDS |
| GGAGAAAGAAGAGGCATAGT+CGG | 0.539953 | 8.3:-7122591 | MS.gene042304:CDS |
| AAGCTGTTAAATTGGGTGAA+AGG | 0.542301 | 8.3:-7123143 | MS.gene042304:CDS |
| GAACATGTCGAGTAGTAAGA+AGG | 0.543783 | 8.3:-7123343 | None:intergenic |
| TGTTAACGCGTATGGTCAGA+AGG | 0.552640 | 8.3:-7123235 | MS.gene042304:CDS |
| AGTGGATCATCGTCTGCCAC+GGG | 0.553302 | 8.3:-7122811 | MS.gene042304:CDS |
| AGAAGGGCCTAGCAGATGGC+AGG | 0.555494 | 8.3:-7123211 | MS.gene042304:CDS |
| TTAAGTTGCTGAGTCCTCGA+TGG | 0.557112 | 8.3:+7123006 | None:intergenic |
| TTTGAGACCGATAGTGGCAG+TGG | 0.557910 | 8.3:-7122829 | MS.gene042304:CDS |
| CAGCTCATCCTCTGAATCAG+AGG | 0.567124 | 8.3:-7122761 | MS.gene042304:CDS |
| AGGAAGAAGGGCCTAGCAGA+TGG | 0.572751 | 8.3:-7123215 | MS.gene042304:CDS |
| ATTCTGATGATAAAAGCAGC+AGG | 0.581802 | 8.3:-7122612 | MS.gene042304:CDS |
| ATTTCAGTGTAAGAATGAGC+GGG | 0.583189 | 8.3:-7123043 | MS.gene042304:CDS |
| TAATAATCATCGTGATGTGA+AGG | 0.585747 | 8.3:-7122926 | MS.gene042304:CDS |
| TGATTCAGGTAGTAATAGTG+AGG | 0.590078 | 8.3:-7122860 | MS.gene042304:CDS |
| CATTTCAGTGTAAGAATGAG+CGG | 0.590562 | 8.3:-7123044 | MS.gene042304:CDS |
| AGGAGAAAAGGGAGGCGAAG+AGG | 0.596956 | 8.3:-7123191 | MS.gene042304:CDS |
| AATCAGAGGAAGAAAGAAGA+CGG | 0.599944 | 8.3:-7122747 | MS.gene042304:CDS |
| GTCAGAAGTGTTACCAGGGT+GGG | 0.604446 | 8.3:-7123074 | MS.gene042304:CDS |
| CAGACTAAGAAGAAGAGCAA+AGG | 0.608854 | 8.3:-7122697 | MS.gene042304:CDS |
| TAATCATCGTGATGTGAAGG+AGG | 0.609023 | 8.3:-7122923 | MS.gene042304:CDS |
| CGTATGGTCAGAAGGAAGAA+GGG | 0.609566 | 8.3:-7123227 | MS.gene042304:CDS |
| AGCCGAAAGAGCAGCAACTG+CGG | 0.616543 | 8.3:-7123268 | MS.gene042304:CDS |
| CCTACAATGTCGACTGTCGA+TGG | 0.624864 | 8.3:-7123108 | MS.gene042304:CDS |
| ATTGAGGCCAGATGTGTCTG+TGG | 0.651230 | 8.3:-7122974 | MS.gene042304:CDS |
| AAAGCAGCAGGAGAAAGAAG+AGG | 0.653056 | 8.3:-7122600 | MS.gene042304:CDS |
| GAAATGTCCAGTGCCCACCC+TGG | 0.658551 | 8.3:+7123061 | None:intergenic |
| TGTCAGAAGTGTTACCAGGG+TGG | 0.665235 | 8.3:-7123075 | MS.gene042304:CDS |
| CAGTGTCAGAAGTGTTACCA+GGG | 0.669839 | 8.3:-7123078 | MS.gene042304:CDS |
| AGAAGAATAATCAGATCCCG+TGG | 0.674048 | 8.3:+7122795 | None:intergenic |
| TTGAGGCCAGATGTGTCTGT+GGG | 0.707588 | 8.3:-7122973 | MS.gene042304:CDS |
| TTTATATTTCAAGGCCATCG+AGG | 0.711495 | 8.3:-7123020 | MS.gene042304:CDS |
| CAGATGGCAGGAGAAAAGGG+AGG | 0.762274 | 8.3:-7123199 | MS.gene042304:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTTAAAAACCCTAAATTG+AGG | - | chr8.3:7122931-7122950 | MS.gene042304:CDS | 25.0% |
| !! | ATCTGATTATTCTTCTAGTT+CGG | - | chr8.3:7123133-7123152 | MS.gene042304:CDS | 25.0% |
| ACTGAGAAAGCTGTTAAATT+GGG | - | chr8.3:7122771-7122790 | MS.gene042304:CDS | 30.0% | |
| TAATAATCATCGTGATGTGA+AGG | - | chr8.3:7122995-7123014 | MS.gene042304:CDS | 30.0% | |
| TACTGAGAAAGCTGTTAAAT+TGG | - | chr8.3:7122770-7122789 | MS.gene042304:CDS | 30.0% | |
| TAGTAAGAAGGAAGAGATTA+AGG | - | chr8.3:7122590-7122609 | MS.gene042304:CDS | 30.0% | |
| !! | TCTGATTATTCTTCTAGTTC+GGG | - | chr8.3:7123134-7123153 | MS.gene042304:CDS | 30.0% |
| AAGCTGTTAAATTGGGTGAA+AGG | - | chr8.3:7122778-7122797 | MS.gene042304:CDS | 35.0% | |
| AATCAGAGGAAGAAAGAAGA+CGG | - | chr8.3:7123174-7123193 | MS.gene042304:CDS | 35.0% | |
| ATTCTGATGATAAAAGCAGC+AGG | - | chr8.3:7123309-7123328 | MS.gene042304:CDS | 35.0% | |
| ATTTCAGTGTAAGAATGAGC+GGG | - | chr8.3:7122878-7122897 | MS.gene042304:CDS | 35.0% | |
| CATTTCAGTGTAAGAATGAG+CGG | - | chr8.3:7122877-7122896 | MS.gene042304:CDS | 35.0% | |
| GAATCATCTGATTCAGATTC+TGG | - | chr8.3:7123275-7123294 | MS.gene042304:CDS | 35.0% | |
| TGATTCAGGTAGTAATAGTG+AGG | - | chr8.3:7123061-7123080 | MS.gene042304:CDS | 35.0% | |
| TGTTAAATTGGGTGAAAGGA+AGG | - | chr8.3:7122782-7122801 | MS.gene042304:CDS | 35.0% | |
| TTTATATTTCAAGGCCATCG+AGG | - | chr8.3:7122901-7122920 | MS.gene042304:CDS | 35.0% | |
| ! | ATGAGCGGGTTTATATTTCA+AGG | - | chr8.3:7122892-7122911 | MS.gene042304:CDS | 35.0% |
| ACACATCTGGCCTCAATTTA+GGG | + | chr8.3:7122944-7122963 | None:intergenic | 40.0% | |
| ACCTGAATCATACTTCCTCT+TGG | + | chr8.3:7123051-7123070 | None:intergenic | 40.0% | |
| AGAAGAATAATCAGATCCCG+TGG | + | chr8.3:7123129-7123148 | None:intergenic | 40.0% | |
| AGAAGAGCAAAGGAAAAAGC+AGG | - | chr8.3:7123234-7123253 | MS.gene042304:CDS | 40.0% | |
| CAGACTAAGAAGAAGAGCAA+AGG | - | chr8.3:7123224-7123243 | MS.gene042304:CDS | 40.0% | |
| TAATCATCGTGATGTGAAGG+AGG | - | chr8.3:7122998-7123017 | MS.gene042304:CDS | 40.0% | |
| TCTTTCTTCCTCTGATTCAG+AGG | + | chr8.3:7123171-7123190 | None:intergenic | 40.0% | |
| TGAGTTCGAAAAAGTCCAAG+AGG | - | chr8.3:7123033-7123052 | MS.gene042304:CDS | 40.0% | |
| ! | TCCAAGAGGAAGTATGATTC+AGG | - | chr8.3:7123047-7123066 | MS.gene042304:CDS | 40.0% |
| ! | TGTGTCTGTGGGTTATGATT+TGG | - | chr8.3:7122959-7122978 | MS.gene042304:CDS | 40.0% |
| !!! | GGACTTTTTCGAACTCACTT+TGG | + | chr8.3:7123030-7123049 | None:intergenic | 40.0% |
| AAAGCAGCAGGAGAAAGAAG+AGG | - | chr8.3:7123321-7123340 | MS.gene042304:CDS | 45.0% | |
| AGAGCAAAGGAAAAAGCAGG+AGG | - | chr8.3:7123237-7123256 | MS.gene042304:CDS | 45.0% | |
| CAGCATTAACTGCTGCCAAA+GGG | - | chr8.3:7122616-7122635 | MS.gene042304:CDS | 45.0% | |
| CGTATGGTCAGAAGGAAGAA+GGG | - | chr8.3:7122694-7122713 | MS.gene042304:CDS | 45.0% | |
| GACACATCTGGCCTCAATTT+AGG | + | chr8.3:7122945-7122964 | None:intergenic | 45.0% | |
| TAGCAGATGGCAGGAGAAAA+GGG | - | chr8.3:7122719-7122738 | MS.gene042304:CDS | 45.0% | |
| TCATAACCCACAGACACATC+TGG | + | chr8.3:7122957-7122976 | None:intergenic | 45.0% | |
| TCGTGATGTGAAGGAGGAAA+AGG | - | chr8.3:7123004-7123023 | MS.gene042304:CDS | 45.0% | |
| TGTTAACGCGTATGGTCAGA+AGG | - | chr8.3:7122686-7122705 | MS.gene042304:CDS | 45.0% | |
| ! | GGAGAAAGAAGAGGCATAGT+CGG | - | chr8.3:7123330-7123349 | MS.gene042304:CDS | 45.0% |
| ! | TCAGTGTTTGAGACCGATAG+TGG | - | chr8.3:7123086-7123105 | MS.gene042304:CDS | 45.0% |
| ! | TTAAGTTGCTGAGTCCTCGA+TGG | + | chr8.3:7122918-7122937 | None:intergenic | 45.0% |
| !! | CAGTGTCAGAAGTGTTACCA+GGG | - | chr8.3:7122843-7122862 | MS.gene042304:CDS | 45.0% |
| ATTGAGGCCAGATGTGTCTG+TGG | - | chr8.3:7122947-7122966 | MS.gene042304:CDS | 50.0% | |
| CAGCTCATCCTCTGAATCAG+AGG | - | chr8.3:7123160-7123179 | MS.gene042304:CDS | 50.0% | |
| CCATCGACAGTCGACATTGT+AGG | + | chr8.3:7122816-7122835 | None:intergenic | 50.0% | |
| CGATGATCCACTGCCACTAT+CGG | + | chr8.3:7123102-7123121 | None:intergenic | 50.0% | |
| CTAGCAGATGGCAGGAGAAA+AGG | - | chr8.3:7122718-7122737 | MS.gene042304:CDS | 50.0% | |
| CTCTGATTCAGAGGATGAGC+TGG | + | chr8.3:7123162-7123181 | None:intergenic | 50.0% | |
| GCAGCATTAACTGCTGCCAA+AGG | - | chr8.3:7122615-7122634 | MS.gene042304:CDS | 50.0% | |
| GCGCGAAATGTTAACGCGTA+TGG | - | chr8.3:7122678-7122697 | MS.gene042304:CDS | 50.0% | |
| GCGTATGGTCAGAAGGAAGA+AGG | - | chr8.3:7122693-7122712 | MS.gene042304:CDS | 50.0% | |
| TTGAGCACGACTAAGCCCTT+TGG | + | chr8.3:7122634-7122653 | None:intergenic | 50.0% | |
| TTGAGGCCAGATGTGTCTGT+GGG | - | chr8.3:7122948-7122967 | MS.gene042304:CDS | 50.0% | |
| ! | CCTACAATGTCGACTGTCGA+TGG | - | chr8.3:7122813-7122832 | MS.gene042304:CDS | 50.0% |
| ! | CTTTTCTCCTGCCATCTGCT+AGG | + | chr8.3:7122720-7122739 | None:intergenic | 50.0% |
| ! | TTTGAGACCGATAGTGGCAG+TGG | - | chr8.3:7123092-7123111 | MS.gene042304:CDS | 50.0% |
| !! | GCAGTGTCAGAAGTGTTACC+AGG | - | chr8.3:7122842-7122861 | MS.gene042304:CDS | 50.0% |
| !! | GTCAGAAGTGTTACCAGGGT+GGG | - | chr8.3:7122847-7122866 | MS.gene042304:CDS | 50.0% |
| !! | TGTCAGAAGTGTTACCAGGG+TGG | - | chr8.3:7122846-7122865 | MS.gene042304:CDS | 50.0% |
| AGCCGAAAGAGCAGCAACTG+CGG | - | chr8.3:7122653-7122672 | MS.gene042304:CDS | 55.0% | |
| AGCCGCAGTTGCTGCTCTTT+CGG | + | chr8.3:7122658-7122677 | None:intergenic | 55.0% | |
| AGGAAGAAGGGCCTAGCAGA+TGG | - | chr8.3:7122706-7122725 | MS.gene042304:CDS | 55.0% | |
| AGGAGAAAAGGGAGGCGAAG+AGG | - | chr8.3:7122730-7122749 | MS.gene042304:CDS | 55.0% | |
| AGTGGATCATCGTCTGCCAC+GGG | - | chr8.3:7123110-7123129 | MS.gene042304:CDS | 55.0% | |
| CAGATGGCAGGAGAAAAGGG+AGG | - | chr8.3:7122722-7122741 | MS.gene042304:CDS | 55.0% | |
| CAGTGGATCATCGTCTGCCA+CGG | - | chr8.3:7123109-7123128 | MS.gene042304:CDS | 55.0% | |
| AGAAGGGCCTAGCAGATGGC+AGG | - | chr8.3:7122710-7122729 | MS.gene042304:CDS | 60.0% | |
| AGTGTTACCAGGGTGGGCAC+TGG | - | chr8.3:7122853-7122872 | MS.gene042304:CDS | 60.0% | |
| GAAATGTCCAGTGCCCACCC+TGG | + | chr8.3:7122863-7122882 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 7122582 | 7123361 | 7122582 | ID=MS.gene042304 |
| chr8.3 | mRNA | 7122582 | 7123361 | 7122582 | ID=MS.gene042304.t1;Parent=MS.gene042304 |
| chr8.3 | exon | 7122582 | 7123361 | 7122582 | ID=MS.gene042304.t1.exon1;Parent=MS.gene042304.t1 |
| chr8.3 | CDS | 7122582 | 7123361 | 7122582 | ID=cds.MS.gene042304.t1;Parent=MS.gene042304.t1 |
| Gene Sequence |
| Protein sequence |
