AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043788

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043788.t1 MTR_2g460780 97.209 215 6 0 1 215 1 215 7.53e-155 429
MS.gene043788.t1 MTR_2g460780 94.570 221 6 1 1 215 1 221 6.25e-152 421
MS.gene043788.t1 MTR_2g460780 93.488 215 6 1 1 215 1 207 6.86e-146 405
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043788.t1 AT5G57950 60.360 222 80 3 1 215 1 221 4.63e-89 262
MS.gene043788.t1 AT5G57950 60.360 222 80 3 1 215 30 250 1.25e-88 262

Find 56 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAACAAATAACAAAATAAT+TGG 0.204859 2.1:-39978215 None:intergenic
TTGGCAAGGCAGAGGTTTAC+TGG 0.266830 2.1:+39978177 MS.gene043788:CDS
CCTGGCGCCCCTGGACTTTC+CGG 0.273188 2.1:+39976206 MS.gene043788:CDS
TTGTTTGACCCATGCTCGAC+TGG 0.343385 2.1:-39978096 None:intergenic
TCTCAATTCCCTGGCGCCCC+TGG 0.352289 2.1:+39976197 MS.gene043788:CDS
CTCAGAATCAATTAGATTGC+CGG 0.364511 2.1:-39976225 None:intergenic
AACAAGTGGAACGTCGATAT+CGG 0.371561 2.1:-39976341 None:intergenic
AGCAGAAACAAGATCTCTAA+TGG 0.389792 2.1:+39976124 MS.gene043788:CDS
ACATCCACATCCATCGAGTT+GGG 0.410980 2.1:-39977926 None:intergenic
ACCCATGCTCGACTGGGCCT+CGG 0.418523 2.1:-39978089 None:intergenic
CCGGAAAGTCCAGGGGCGCC+AGG 0.421638 2.1:-39976206 None:intergenic
TTCCGAGGCCCAGTCGAGCA+TGG 0.452146 2.1:+39978087 MS.gene043788:CDS
GCTCGTCTTTCTCAATTCCC+TGG 0.456059 2.1:+39976188 MS.gene043788:CDS
TGAAAATTTGCTCCATAGGT+TGG 0.465291 2.1:+39978063 MS.gene043788:CDS
AAATTTGGCAACGTCGAAGC+AGG 0.480789 2.1:+39978040 MS.gene043788:CDS
TAACAAATAACAAAATAATT+GGG 0.487430 2.1:-39978214 None:intergenic
ATTAGATTGCCGGAAAGTCC+AGG 0.487468 2.1:-39976215 None:intergenic
ATCTCGTCAACCACTGCAAA+GGG 0.491382 2.1:-39977962 None:intergenic
CCTCTGCCTTGCCAAGTCCT+AGG 0.496210 2.1:-39978169 None:intergenic
CGGCAATCTAATTGATTCTG+AGG 0.497483 2.1:+39976226 MS.gene043788:CDS
GTAGAAGATGGTTTACAGCT+TGG 0.500978 2.1:+39978004 MS.gene043788:CDS
GGGCCTCGGAAGCCAACCTA+TGG 0.501119 2.1:-39978075 None:intergenic
CAGGTGAAAATTTGCTCCAT+AGG 0.501246 2.1:+39978059 MS.gene043788:CDS
TGGCAAGGCAGAGGTTTACT+GGG 0.504955 2.1:+39978178 MS.gene043788:CDS
CTCGATGGATGTGGATGTCT+TGG 0.513868 2.1:+39977931 MS.gene043788:CDS
ATTAATCTTATCAGTTATGT+CGG 0.522206 2.1:-39976407 None:intergenic
TCCGAGGCCCAGTCGAGCAT+GGG 0.530910 2.1:+39978088 MS.gene043788:CDS
GACATCCACATCCATCGAGT+TGG 0.533268 2.1:-39977927 None:intergenic
CCTTGCCTCATGATGACAAC+AGG 0.536038 2.1:-39978121 None:intergenic
GCTCCATAGGTTGGCTTCCG+AGG 0.537445 2.1:+39978072 MS.gene043788:CDS
AATAACAAAATAATTGGGAC+AGG 0.549596 2.1:-39978209 None:intergenic
TTTATGATTTCCAAGTCTTG+AGG 0.549764 2.1:-39976452 None:intergenic
TGTTTGACCCATGCTCGACT+GGG 0.552241 2.1:-39978095 None:intergenic
AGTAGAAGAAGAAGAATGGT+GGG 0.556057 2.1:+39976089 None:intergenic
GCATCACCAGCGGTAGAAGA+TGG 0.559452 2.1:+39977992 MS.gene043788:CDS
CGTCGTTCAGCTCGAACAAG+TGG 0.560165 2.1:-39976355 None:intergenic
CCTAGGACTTGGCAAGGCAG+AGG 0.560284 2.1:+39978169 MS.gene043788:CDS
TGTAAACCATCTTCTACCGC+TGG 0.569610 2.1:-39977998 None:intergenic
CGGAAAGTCCAGGGGCGCCA+GGG 0.574777 2.1:-39976205 None:intergenic
TATCTCGTCAACCACTGCAA+AGG 0.575740 2.1:-39977963 None:intergenic
TAACTGTTACACCTAGGACT+TGG 0.577662 2.1:+39978158 MS.gene043788:CDS
CATCCACATCCATCGAGTTG+GGG 0.579168 2.1:-39977925 None:intergenic
TTAGATTGCCGGAAAGTCCA+GGG 0.588856 2.1:-39976214 None:intergenic
CAAGATCTCTAATGGATAAG+CGG 0.592560 2.1:+39976132 MS.gene043788:CDS
AACAGTTTCTGCAGTAGATG+AGG 0.592840 2.1:-39977864 None:intergenic
GGTTAGTAGACCCTTTGCAG+TGG 0.593357 2.1:+39977952 MS.gene043788:CDS
CAATACCTGTTGTCATCATG+AGG 0.605657 2.1:+39978116 MS.gene043788:CDS
CCTGTTGTCATCATGAGGCA+AGG 0.626933 2.1:+39978121 MS.gene043788:CDS
GTTACACCTAGGACTTGGCA+AGG 0.647181 2.1:+39978163 MS.gene043788:CDS
CTCACAAGGACATTCTGTGA+TGG 0.663425 2.1:-39977896 None:intergenic
ATCCACATCCATCGAGTTGG+GGG 0.663720 2.1:-39977924 None:intergenic
TCAACTTAACTGTTACACCT+AGG 0.680007 2.1:+39978152 MS.gene043788:CDS
TAGATTGCCGGAAAGTCCAG+GGG 0.688081 2.1:-39976213 None:intergenic
GGCAAGGCAGAGGTTTACTG+GGG 0.698130 2.1:+39978179 MS.gene043788:CDS
GATAGCAGATGCATCACCAG+CGG 0.721340 2.1:+39977982 MS.gene043788:CDS
GGAACGTCGATATCGGAACG+AGG 0.736140 2.1:-39976334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACAAATAACAAAATAAT+TGG - chr2.1:39978218-39978237 None:intergenic 10.0%
!! ATAATTAATAGACAATAAAT+TGG + chr2.1:39977614-39977633 MS.gene043788:intron 10.0%
!! TAACAAATAACAAAATAATT+GGG - chr2.1:39978217-39978236 None:intergenic 10.0%
!!! TCATTTTCTTAAATTATTAT+TGG - chr2.1:39976542-39976561 None:intergenic 10.0%
!! AACTTCAAAAATATCAATAA+TGG - chr2.1:39976937-39976956 None:intergenic 15.0%
!! ATTCATAATATGAAACAAAA+GGG - chr2.1:39977341-39977360 None:intergenic 15.0%
!! TATTCATAATATGAAACAAA+AGG - chr2.1:39977342-39977361 None:intergenic 15.0%
!! TCTAGTATTAAATTTATTAG+TGG + chr2.1:39977423-39977442 MS.gene043788:intron 15.0%
!! TTATATATGAAAAGCTTAAT+AGG - chr2.1:39976906-39976925 None:intergenic 15.0%
!!! GTTATTGTTTTACTATTTAT+AGG - chr2.1:39976690-39976709 None:intergenic 15.0%
!! ATTAATCTTATCAGTTATGT+CGG - chr2.1:39976410-39976429 None:intergenic 20.0%
!! TAAAGCCTATTTAATTAAAC+AGG + chr2.1:39976835-39976854 MS.gene043788:intron 20.0%
!! TAAATAATGAATTCTTACGA+AGG - chr2.1:39977025-39977044 None:intergenic 20.0%
!! TTATTTAGTAGCTGATATAT+TGG + chr2.1:39976989-39977008 MS.gene043788:intron 20.0%
!!! CATAAAATAGGCTTTTAAAT+AGG + chr2.1:39977128-39977147 MS.gene043788:intron 20.0%
!!! CATAAATATTGTTTTCTGAA+TGG + chr2.1:39977730-39977749 MS.gene043788:intron 20.0%
!!! GAAATTTTGAGTAATACTTA+AGG + chr2.1:39977368-39977387 MS.gene043788:intron 20.0%
!!! GTATTTCTTTTTGAATAGTA+TGG + chr2.1:39977398-39977417 MS.gene043788:intron 20.0%
! AAAACATATTTGTGTGCTTT+AGG + chr2.1:39977049-39977068 MS.gene043788:intron 25.0%
! AAATAGGCCAAAACTTATTA+AGG - chr2.1:39977075-39977094 None:intergenic 25.0%
! AATAACAAAATAATTGGGAC+AGG - chr2.1:39978212-39978231 None:intergenic 25.0%
! ATACCAAATTACATACAACT+AGG - chr2.1:39977296-39977315 None:intergenic 25.0%
! CTAAATAACCAATCATATGA+AGG - chr2.1:39976977-39976996 None:intergenic 25.0%
! CTTGGAAATCATAAAAATTC+AGG + chr2.1:39976460-39976479 MS.gene043788:CDS 25.0%
! GAAATCATAAAAATTCAGGT+TGG + chr2.1:39976464-39976483 MS.gene043788:intron 25.0%
! TACCAAATTACATACAACTA+GGG - chr2.1:39977295-39977314 None:intergenic 25.0%
!! TAAAAGCCTATTTTATGCTA+AGG - chr2.1:39977125-39977144 None:intergenic 25.0%
!!! GGAGATCAAATTTTGAAATT+TGG + chr2.1:39978025-39978044 MS.gene043788:CDS 25.0%
!!! TAATAAGTTTTGGCCTATTT+AGG + chr2.1:39977075-39977094 MS.gene043788:intron 25.0%
AACTTGTACTAGTTGTATGT+AGG + chr2.1:39976707-39976726 MS.gene043788:intron 30.0%
ACCAAATTACATACAACTAG+GGG - chr2.1:39977294-39977313 None:intergenic 30.0%
ACCTTGTTAATAAATGAGCT+AGG - chr2.1:39976798-39976817 None:intergenic 30.0%
ACTTGTACTAGTTGTATGTA+GGG + chr2.1:39976708-39976727 MS.gene043788:intron 30.0%
ATATGAAAAGCTTAATAGGC+CGG - chr2.1:39976902-39976921 None:intergenic 30.0%
ATGTAAACCTACTCCTAAAT+AGG - chr2.1:39977091-39977110 None:intergenic 30.0%
TGGCCTACTTCAAAAATTAT+AGG - chr2.1:39977228-39977247 None:intergenic 30.0%
TTCCACTAAGACAATTATGA+TGG + chr2.1:39977827-39977846 MS.gene043788:intron 30.0%
TTTATGATTTCCAAGTCTTG+AGG - chr2.1:39976455-39976474 None:intergenic 30.0%
! CTTTAGGCCTTAATAAGTTT+TGG + chr2.1:39977065-39977084 MS.gene043788:intron 30.0%
!!! AGGCCTATAATTTTTGAAGT+AGG + chr2.1:39977222-39977241 MS.gene043788:intron 30.0%
!!! TATAATTTTTGAAGTAGGCC+AGG + chr2.1:39977227-39977246 MS.gene043788:intron 30.0%
AAAAATTATAGGCCTGAGTC+TGG - chr2.1:39977217-39977236 None:intergenic 35.0%
AAATTACATACAACTAGGGG+TGG - chr2.1:39977291-39977310 None:intergenic 35.0%
AACACAAGCCTTCATATGAT+TGG + chr2.1:39976966-39976985 MS.gene043788:intron 35.0%
AACATCTCTGTTGTCAAAAG+CGG + chr2.1:39976576-39976595 MS.gene043788:intron 35.0%
ACCCCTAGTTGTATGTAATT+TGG + chr2.1:39977290-39977309 MS.gene043788:intron 35.0%
AGCAGAAACAAGATCTCTAA+TGG + chr2.1:39976124-39976143 MS.gene043788:CDS 35.0%
AGCCATCATAATTGTCTTAG+TGG - chr2.1:39977832-39977851 None:intergenic 35.0%
AGGGATAGCAAAATCGAAAA+TGG - chr2.1:39977322-39977341 None:intergenic 35.0%
CCTATTTAATTAAACAGGCC+AGG + chr2.1:39976840-39976859 MS.gene043788:intron 35.0%
CCTGGCCTGTTTAATTAAAT+AGG - chr2.1:39976843-39976862 None:intergenic 35.0%
CTCAGAATCAATTAGATTGC+CGG - chr2.1:39976228-39976247 None:intergenic 35.0%
CTTGTACTAGTTGTATGTAG+GGG + chr2.1:39976709-39976728 MS.gene043788:intron 35.0%
GCCTAGCTCATTTATTAACA+AGG + chr2.1:39976794-39976813 MS.gene043788:intron 35.0%
TAATGAAAACCTTCACTCCA+TGG - chr2.1:39977595-39977614 None:intergenic 35.0%
TACTATTTATAGGTCCGCTT+TGG - chr2.1:39976680-39976699 None:intergenic 35.0%
TCAACTTAACTGTTACACCT+AGG + chr2.1:39978152-39978171 MS.gene043788:CDS 35.0%
TTGTACTAGTTGTATGTAGG+GGG + chr2.1:39976710-39976729 MS.gene043788:intron 35.0%
! AGGCTTTTAAATAGGCTTTC+AGG + chr2.1:39977136-39977155 MS.gene043788:intron 35.0%
! ATTTTGCATTCCTCAAGACT+TGG + chr2.1:39976442-39976461 MS.gene043788:CDS 35.0%
! CAAGATCTCTAATGGATAAG+CGG + chr2.1:39976132-39976151 MS.gene043788:CDS 35.0%
! TGAAAATTTGCTCCATAGGT+TGG + chr2.1:39978063-39978082 MS.gene043788:CDS 35.0%
! TTCACTCCATGGATTCTTTT+CGG - chr2.1:39977584-39977603 None:intergenic 35.0%
! TTTTCGCCTGGCATTTTTAA+AGG - chr2.1:39977172-39977191 None:intergenic 35.0%
!! ATGGATGGATTGAAAGAAGA+AGG - chr2.1:39976285-39976304 None:intergenic 35.0%
!! TGGATGGATTGAAAGAAGAA+GGG - chr2.1:39976284-39976303 None:intergenic 35.0%
AAAAGCCTATTAAGCCCATC+AGG + chr2.1:39976876-39976895 MS.gene043788:intron 40.0%
AACAAGTGGAACGTCGATAT+CGG - chr2.1:39976344-39976363 None:intergenic 40.0%
AACAGTTTCTGCAGTAGATG+AGG - chr2.1:39977867-39977886 None:intergenic 40.0%
AGAGAGAGAGAGAGTTATGA+TGG - chr2.1:39976304-39976323 None:intergenic 40.0%
ATAACTCTCTCTCTCTCTCT+AGG + chr2.1:39976306-39976325 MS.gene043788:intron 40.0%
ATACAACTAGGGGTGGAAAT+GGG - chr2.1:39977284-39977303 None:intergenic 40.0%
CAATACCTGTTGTCATCATG+AGG + chr2.1:39978116-39978135 MS.gene043788:CDS 40.0%
CCTAATCCGAAAAGAATCCA+TGG + chr2.1:39977575-39977594 MS.gene043788:intron 40.0%
GAAAAGAATCCATGGAGTGA+AGG + chr2.1:39977583-39977602 MS.gene043788:intron 40.0%
GTAAACATACGTGTCAGTGT+CGG + chr2.1:39977540-39977559 MS.gene043788:intron 40.0%
GTAGAAGATGGTTTACAGCT+TGG + chr2.1:39978004-39978023 MS.gene043788:CDS 40.0%
GTAGGTTTACATATGTTGCC+AGG + chr2.1:39977099-39977118 MS.gene043788:intron 40.0%
GTTTACCTCTGTTGTCAATG+CGG - chr2.1:39977526-39977545 None:intergenic 40.0%
TAAACATACGTGTCAGTGTC+GGG + chr2.1:39977541-39977560 MS.gene043788:intron 40.0%
TAACTCTCTCTCTCTCTCTA+GGG + chr2.1:39976307-39976326 MS.gene043788:intron 40.0%
TAACTGTTACACCTAGGACT+TGG + chr2.1:39978158-39978177 MS.gene043788:CDS 40.0%
TAATTAAACAGGCCAGGCTA+AGG + chr2.1:39976846-39976865 MS.gene043788:intron 40.0%
TATTTATAGGTCCGCTTTGG+CGG - chr2.1:39976677-39976696 None:intergenic 40.0%
! AGAGAGAGAGTTATGATGGA+TGG - chr2.1:39976300-39976319 None:intergenic 40.0%
! AGCCTATTTAAAGCCTAGCT+CGG + chr2.1:39976772-39976791 MS.gene043788:intron 40.0%
! ATCAGGCCTTTAAAAATGCC+AGG + chr2.1:39977163-39977182 MS.gene043788:intron 40.0%
! CAGGTGAAAATTTGCTCCAT+AGG + chr2.1:39978059-39978078 MS.gene043788:CDS 40.0%
! CCATGGATTCTTTTCGGATT+AGG - chr2.1:39977578-39977597 None:intergenic 40.0%
! CGGCAATCTAATTGATTCTG+AGG + chr2.1:39976226-39976245 MS.gene043788:CDS 40.0%
!! AGCTAGGCTTTAAATAGGCT+GGG - chr2.1:39976772-39976791 None:intergenic 40.0%
!! GTTTTGGCCTATTTAGGAGT+AGG + chr2.1:39977081-39977100 MS.gene043788:intron 40.0%
AAAATCAAGACCACCGCATC+AGG + chr2.1:39976626-39976645 MS.gene043788:intron 45.0%
AAATTTGGCAACGTCGAAGC+AGG + chr2.1:39978040-39978059 MS.gene043788:CDS 45.0%
ACATCCACATCCATCGAGTT+GGG - chr2.1:39977929-39977948 None:intergenic 45.0%
ACCCAATAATTGACGCAGTG+CGG - chr2.1:39977487-39977506 None:intergenic 45.0%
ACTAGTTGTATGTAGGGGGT+GGG + chr2.1:39976714-39976733 MS.gene043788:intron 45.0%
AGGCGAAAATGAGCCTATGA+CGG + chr2.1:39977183-39977202 MS.gene043788:intron 45.0%
ATAGGCTTTCAGGCTTCATC+AGG + chr2.1:39977146-39977165 MS.gene043788:intron 45.0%
ATCTCGTCAACCACTGCAAA+GGG - chr2.1:39977965-39977984 None:intergenic 45.0%
ATTAGATTGCCGGAAAGTCC+AGG - chr2.1:39976218-39976237 None:intergenic 45.0%
CACTGCGTCAATTATTGGGT+TGG + chr2.1:39977487-39977506 MS.gene043788:intron 45.0%
CATACAACTAGGGGTGGAAA+TGG - chr2.1:39977285-39977304 None:intergenic 45.0%
CTCACAAGGACATTCTGTGA+TGG - chr2.1:39977899-39977918 None:intergenic 45.0%
CTGTTGTCAATGCGGAAATG+CGG - chr2.1:39977518-39977537 None:intergenic 45.0%
CTTCGTGTCTCGTGTTTCAT+AGG - chr2.1:39976503-39976522 None:intergenic 45.0%
GCCAGGCCTTAGCATAAAAT+AGG + chr2.1:39977116-39977135 MS.gene043788:intron 45.0%
TACTAGTTGTATGTAGGGGG+TGG + chr2.1:39976713-39976732 MS.gene043788:intron 45.0%
TATCTCGTCAACCACTGCAA+AGG - chr2.1:39977966-39977985 None:intergenic 45.0%
TGTAAACCATCTTCTACCGC+TGG - chr2.1:39978001-39978020 None:intergenic 45.0%
TTAGATTGCCGGAAAGTCCA+GGG - chr2.1:39976217-39976236 None:intergenic 45.0%
! ATTTTTCCGCTCCGCTATAG+CGG - chr2.1:39976611-39976630 None:intergenic 45.0%
! CATTTCCGCATTGACAACAG+AGG + chr2.1:39977518-39977537 MS.gene043788:intron 45.0%
! GCCTATTTTATGCTAAGGCC+TGG - chr2.1:39977120-39977139 None:intergenic 45.0%
!! ATGAGCCTATGACGGGTAAT+AGG + chr2.1:39977191-39977210 MS.gene043788:intron 45.0%
!! GAGCTAGGCTTTAAATAGGC+TGG - chr2.1:39976773-39976792 None:intergenic 45.0%
!!! CTTTTTTGCAAGCCTTAGCC+TGG - chr2.1:39976861-39976880 None:intergenic 45.0%
!!! TTTTGAAGTAGGCCAGGCTT+AGG + chr2.1:39977233-39977252 MS.gene043788:intron 45.0%
AGTTGGGGGAAAGACTCACA+AGG - chr2.1:39977913-39977932 None:intergenic 50.0%
CCCAATAATTGACGCAGTGC+GGG - chr2.1:39977486-39977505 None:intergenic 50.0%
CCCGCACTGCGTCAATTATT+GGG + chr2.1:39977483-39977502 MS.gene043788:intron 50.0%
CCTGTTGTCATCATGAGGCA+AGG + chr2.1:39978121-39978140 MS.gene043788:CDS 50.0%
CCTTGCCTCATGATGACAAC+AGG - chr2.1:39978124-39978143 None:intergenic 50.0%
CTCGATGGATGTGGATGTCT+TGG + chr2.1:39977931-39977950 MS.gene043788:CDS 50.0%
CTTTAAATAGGCTGGGCCGT+AGG - chr2.1:39976765-39976784 None:intergenic 50.0%
GACATCCACATCCATCGAGT+TGG - chr2.1:39977930-39977949 None:intergenic 50.0%
GATAGCAGATGCATCACCAG+CGG + chr2.1:39977982-39978001 MS.gene043788:CDS 50.0%
GCTCGTCTTTCTCAATTCCC+TGG + chr2.1:39976188-39976207 MS.gene043788:CDS 50.0%
GGCGAAAATGAGCCTATGAC+GGG + chr2.1:39977184-39977203 MS.gene043788:intron 50.0%
GGTTAGTAGACCCTTTGCAG+TGG + chr2.1:39977952-39977971 MS.gene043788:CDS 50.0%
GTTACACCTAGGACTTGGCA+AGG + chr2.1:39978163-39978182 MS.gene043788:CDS 50.0%
TAAATGAGCTAGGCCGAGCT+AGG - chr2.1:39976788-39976807 None:intergenic 50.0%
TAGATTGCCGGAAAGTCCAG+GGG - chr2.1:39976216-39976235 None:intergenic 50.0%
TCCCGCACTGCGTCAATTAT+TGG + chr2.1:39977482-39977501 MS.gene043788:intron 50.0%
TCTGGCCTATTACCCGTCAT+AGG - chr2.1:39977199-39977218 None:intergenic 50.0%
TGGCAAGGCAGAGGTTTACT+GGG + chr2.1:39978178-39978197 MS.gene043788:CDS 50.0%
TGTTTGACCCATGCTCGACT+GGG - chr2.1:39978098-39978117 None:intergenic 50.0%
TTGGCAAGGCAGAGGTTTAC+TGG + chr2.1:39978177-39978196 MS.gene043788:CDS 50.0%
TTGTTTGACCCATGCTCGAC+TGG - chr2.1:39978099-39978118 None:intergenic 50.0%
! CATCCACATCCATCGAGTTG+GGG - chr2.1:39977928-39977947 None:intergenic 50.0%
! GTCATAGGCTCATTTTCGCC+TGG - chr2.1:39977184-39977203 None:intergenic 50.0%
!! ATCCACATCCATCGAGTTGG+GGG - chr2.1:39977927-39977946 None:intergenic 50.0%
!! GGCCGAGCTAGGCTTTAAAT+AGG - chr2.1:39976777-39976796 None:intergenic 50.0%
AATTGACGCAGTGCGGGAGT+GGG - chr2.1:39977480-39977499 None:intergenic 55.0%
ACGGGTAATAGGCCAGACTC+AGG + chr2.1:39977202-39977221 MS.gene043788:intron 55.0%
AGCTTAATAGGCCGGCCTGA+TGG - chr2.1:39976894-39976913 None:intergenic 55.0%
CGTCGTTCAGCTCGAACAAG+TGG - chr2.1:39976358-39976377 None:intergenic 55.0%
GAAGTGGAAAGCCGCCAAAG+CGG + chr2.1:39976663-39976682 MS.gene043788:intron 55.0%
GAGTCTTTCCCCCAACTCGA+TGG + chr2.1:39977916-39977935 MS.gene043788:CDS 55.0%
GCATCACCAGCGGTAGAAGA+TGG + chr2.1:39977992-39978011 MS.gene043788:CDS 55.0%
GCCTATTAAGCCCATCAGGC+CGG + chr2.1:39976880-39976899 MS.gene043788:intron 55.0%
GCTTAATAGGCCGGCCTGAT+GGG - chr2.1:39976893-39976912 None:intergenic 55.0%
GGAACGTCGATATCGGAACG+AGG - chr2.1:39976337-39976356 None:intergenic 55.0%
GGCAAGGCAGAGGTTTACTG+GGG + chr2.1:39978179-39978198 MS.gene043788:CDS 55.0%
GGCTTAGGCCTTACAAAGCC+CGG + chr2.1:39977248-39977267 MS.gene043788:intron 55.0%
GTTGTCAAAAGCGGTTGCGC+CGG + chr2.1:39976585-39976604 MS.gene043788:intron 55.0%
TAATTGACGCAGTGCGGGAG+TGG - chr2.1:39977481-39977500 None:intergenic 55.0%
TGTATGTAGGGGGTGGGAAC+AGG + chr2.1:39976720-39976739 MS.gene043788:intron 55.0%
TTCCCCCAACTCGATGGATG+TGG + chr2.1:39977922-39977941 MS.gene043788:CDS 55.0%
! ACTAGGGGTGGAAATGGGCT+AGG - chr2.1:39977279-39977298 None:intergenic 55.0%
! ATACGTGTCAGTGTCGGGCA+CGG + chr2.1:39977546-39977565 MS.gene043788:intron 55.0%
! GGCTTTGTAAGGCCTAAGCC+TGG - chr2.1:39977248-39977267 None:intergenic 55.0%
ATTGACGCAGTGCGGGAGTG+GGG - chr2.1:39977479-39977498 None:intergenic 60.0%
CCTAGGACTTGGCAAGGCAG+AGG + chr2.1:39978169-39978188 MS.gene043788:CDS 60.0%
CCTCTGCCTTGCCAAGTCCT+AGG - chr2.1:39978172-39978191 None:intergenic 60.0%
GTCAATGCGGAAATGCGGCG+CGG - chr2.1:39977513-39977532 None:intergenic 60.0%
! TACGTGTCAGTGTCGGGCAC+GGG + chr2.1:39977547-39977566 MS.gene043788:intron 60.0%
!! GCCGGCCTGATGGGCTTAAT+AGG - chr2.1:39976884-39976903 None:intergenic 60.0%
!! GCTCCATAGGTTGGCTTCCG+AGG + chr2.1:39978072-39978091 MS.gene043788:CDS 60.0%
AACAGGCCAGGACGTCCGAC+AGG + chr2.1:39976737-39976756 MS.gene043788:intron 65.0%
ACAGGCCAGGACGTCCGACA+GGG + chr2.1:39976738-39976757 MS.gene043788:intron 65.0%
ACCCATGCTCGACTGGGCCT+CGG - chr2.1:39978092-39978111 None:intergenic 65.0%
AGGCCTTACAAAGCCCGGCC+CGG + chr2.1:39977253-39977272 MS.gene043788:intron 65.0%
GACGTGCGCGTAGCCTGATG+CGG - chr2.1:39976642-39976661 None:intergenic 65.0%
GGCTACGCGCACGTCAGAAG+TGG + chr2.1:39976647-39976666 MS.gene043788:intron 65.0%
GGGCCTCGGAAGCCAACCTA+TGG - chr2.1:39978078-39978097 None:intergenic 65.0%
TCCGAGGCCCAGTCGAGCAT+GGG + chr2.1:39978088-39978107 MS.gene043788:CDS 65.0%
TCTCAATTCCCTGGCGCCCC+TGG + chr2.1:39976197-39976216 MS.gene043788:CDS 65.0%
TTCCGAGGCCCAGTCGAGCA+TGG + chr2.1:39978087-39978106 MS.gene043788:CDS 65.0%
TTCCGCTCCGCTATAGCGGC+CGG - chr2.1:39976607-39976626 None:intergenic 65.0%
!! GCGCGTAGCGGGCGCTATTT+CGG - chr2.1:39977457-39977476 None:intergenic 65.0%
!! GGGGTGGAAATGGGCTAGGC+CGG - chr2.1:39977275-39977294 None:intergenic 65.0%
!! GGGTGGAAATGGGCTAGGCC+GGG - chr2.1:39977274-39977293 None:intergenic 65.0%
CAGGCCAGGACGTCCGACAG+GGG + chr2.1:39976739-39976758 MS.gene043788:intron 70.0%
CCTGGCGCCCCTGGACTTTC+CGG + chr2.1:39976206-39976225 MS.gene043788:CDS 70.0%
GGACGTCCGACAGGGGCCTA+CGG + chr2.1:39976746-39976765 MS.gene043788:intron 70.0%
GTAGGGGGTGGGAACAGGCC+AGG + chr2.1:39976725-39976744 MS.gene043788:intron 70.0%
GTGCGCGTAGCCTGATGCGG+TGG - chr2.1:39976639-39976658 None:intergenic 70.0%
TAGGCCCCTGTCGGACGTCC+TGG - chr2.1:39976746-39976765 None:intergenic 70.0%
! AGGCCGGGCCGGGCTTTGTA+AGG - chr2.1:39977259-39977278 None:intergenic 70.0%
! GGTTGCGCCGGCCGCTATAG+CGG + chr2.1:39976597-39976616 MS.gene043788:intron 70.0%
!! CGGAAAGTCCAGGGGCGCCA+GGG - chr2.1:39976208-39976227 None:intergenic 70.0%
!! GAAATGGGCTAGGCCGGGCC+GGG - chr2.1:39977269-39977288 None:intergenic 70.0%
!! GGAAATGGGCTAGGCCGGGC+CGG - chr2.1:39977270-39977289 None:intergenic 70.0%
GCTGGGCCGTAGGCCCCTGT+CGG - chr2.1:39976755-39976774 None:intergenic 75.0%
TGCGGGAGTGGGGCGCGTAG+CGG - chr2.1:39977469-39977488 None:intergenic 75.0%
! CGCCGGCCGCTATAGCGGAG+CGG + chr2.1:39976602-39976621 MS.gene043788:intron 75.0%
!! CCGGAAAGTCCAGGGGCGCC+AGG - chr2.1:39976209-39976228 None:intergenic 75.0%
! GCGGGAGTGGGGCGCGTAGC+GGG - chr2.1:39977468-39977487 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 39976104 39978252 39976104 ID=MS.gene043788
chr2.1 mRNA 39976104 39978252 39976104 ID=MS.gene043788.t1;Parent=MS.gene043788
chr2.1 exon 39976104 39976247 39976104 ID=MS.gene043788.t1.exon1;Parent=MS.gene043788.t1
chr2.1 CDS 39976104 39976247 39976104 ID=cds.MS.gene043788.t1;Parent=MS.gene043788.t1
chr2.1 exon 39976328 39976481 39976328 ID=MS.gene043788.t1.exon2;Parent=MS.gene043788.t1
chr2.1 CDS 39976328 39976481 39976328 ID=cds.MS.gene043788.t1;Parent=MS.gene043788.t1
chr2.1 exon 39977855 39978252 39977855 ID=MS.gene043788.t1.exon3;Parent=MS.gene043788.t1
chr2.1 CDS 39977855 39978252 39977855 ID=cds.MS.gene043788.t1;Parent=MS.gene043788.t1
Gene Sequence

>MS.gene043788

ATGGTGGGAACGAACGTGAAAGCAGAAACAAGATCTCTAATGGATAAGCGGAGTGCGTTAGAGTCTGAAATGAACTCAATTATCGCTCGTCTTTCTCAATTCCCTGGCGCCCCTGGACTTTCCGGCAATCTAATTGATTCTGAGGTTTGTTTCTCTGCAAATTATTTCATTCAAATACCCTTCTTCTTTCAATCCATCCATCATAACTCTCTCTCTCTCTCTAGGGTTTTCCTCGTTCCGATATCGACGTTCCACTTGTTCGAGCTGAACGACGTCGTCTTACTGAGCTGCGTAATGACTACACCGACATAACTGATAAGATTAATCAAAATATACACATTTTGCATTCCTCAAGACTTGGAAATCATAAAAATTCAGGTTGGTTTACTTCTCAAACCTATGAAACACGAGACACGAAGCACATTCACGAACACACCAATAATAATTTAAGAAAATGACATAATTCAATGTAAACATCTCTGTTGTCAAAAGCGGTTGCGCCGGCCGCTATAGCGGAGCGGAAAAATCAAGACCACCGCATCAGGCTACGCGCACGTCAGAAGTGGAAAGCCGCCAAAGCGGACCTATAAATAGTAAAACAATAACTTGTACTAGTTGTATGTAGGGGGTGGGAACAGGCCAGGACGTCCGACAGGGGCCTACGGCCCAGCCTATTTAAAGCCTAGCTCGGCCTAGCTCATTTATTAACAAGGTCAGACTCAAGCTTTTTATAAAGCCTATTTAATTAAACAGGCCAGGCTAAGGCTTGCAAAAAAGCCTATTAAGCCCATCAGGCCGGCCTATTAAGCTTTTCATATATAATATATCTTCCATTATTGATATTTTTGAAGTTTTTTTCATAAACACAAGCCTTCATATGATTGGTTATTTAGTAGCTGATATATTGGTGTGATTAGACCTTCGTAAGAATTCATTATTTAGTAGAAAACATATTTGTGTGCTTTAGGCCTTAATAAGTTTTGGCCTATTTAGGAGTAGGTTTACATATGTTGCCAGGCCTTAGCATAAAATAGGCTTTTAAATAGGCTTTCAGGCTTCATCAGGCCTTTAAAAATGCCAGGCGAAAATGAGCCTATGACGGGTAATAGGCCAGACTCAGGCCTATAATTTTTGAAGTAGGCCAGGCTTAGGCCTTACAAAGCCCGGCCCGGCCTAGCCCATTTCCACCCCTAGTTGTATGTAATTTGGTATAAGCCATTTTCGATTTTGCTATCCCTTTTGTTTCATATTATGAATACTTCATGAAATTTTGAGTAATACTTAAGGTATTTTAGTATTTCTTTTTGAATAGTATGGTATCTAGTATTAAATTTATTAGTGGCTGCATCACCGAAATAGCGCCCGCTACGCGCCCCACTCCCGCACTGCGTCAATTATTGGGTTGGCCGCGCCGCATTTCCGCATTGACAACAGAGGTAAACATACGTGTCAGTGTCGGGCACGGGACACACCTAATCCGAAAAGAATCCATGGAGTGAAGGTTTTCATTATAATTAATAGACAATAAATTGGCTTCTGAGAAGCATTCTGTTTTGTTCTTGCTTAACAAAAGATGATAATTCGCTGTTGCATTTTTTTACTGAAGTAATCAAATTTCAGTTTTTGCATAAATATTGTTTTCTGAATGGTGAACTTCTGTATTAACTTTTAATGTTATTTAATATTAATATCATATTTGTTATGTCATGTTGAATGTTTGCTTTTCCACTAAGACAATTATGATGGCTCAGACATGCAGACCTCATCTACTGCAGAAACTGTTGCATCAACACCATCACAGAATGTCCTTGTGAGTCTTTCCCCCAACTCGATGGATGTGGATGTCTTGGTTAGTAGACCCTTTGCAGTGGTTGACGAGATAGCAGATGCATCACCAGCGGTAGAAGATGGTTTACAGCTTGGAGATCAAATTTTGAAATTTGGCAACGTCGAAGCAGGTGAAAATTTGCTCCATAGGTTGGCTTCCGAGGCCCAGTCGAGCATGGGTCAAACAATACCTGTTGTCATCATGAGGCAAGGCACAGTGATCAACTTAACTGTTACACCTAGGACTTGGCAAGGCAGAGGTTTACTGGGGTATGCTTCCTGTCCCAATTATTTTGTTATTTGTTATCTTGTGTGCTTATAG
Protein sequence

>MS.gene043788.t1

MVGTNVKAETRSLMDKRSALESEMNSIIARLSQFPGAPGLSGNLIDSEGFPRSDIDVPLVRAERRRLTELRNDYTDITDKINQNIHILHSSRLGNHKNSDMQTSSTAETVASTPSQNVLVSLSPNSMDVDVLVSRPFAVVDEIADASPAVEDGLQLGDQILKFGNVEAGENLLHRLASEAQSSMGQTIPVVIMRQGTVINLTVTPRTWQGRGLLGYASCPNYFVICYLVCL