AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047951

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047951.t1 MTR_2g022700 96.212 396 10 1 1 391 1 396 0.0 765
MS.gene047951.t1 MTR_4g131800 75.904 415 73 3 1 389 1 414 0.0 577
MS.gene047951.t1 MTR_3g049400 76.064 376 74 6 26 390 31 401 0.0 553
MS.gene047951.t1 MTR_2g029180 49.555 337 157 7 54 387 66 392 3.33e-96 294
MS.gene047951.t1 MTR_4g088350 43.434 297 159 4 97 389 97 388 6.55e-78 247
MS.gene047951.t1 MTR_5g044220 37.653 409 219 10 5 391 1 395 3.31e-77 245
MS.gene047951.t1 MTR_6g005480 44.242 330 165 5 67 390 92 408 7.36e-76 242
MS.gene047951.t1 MTR_4g105630 41.611 298 171 2 95 390 81 377 5.51e-71 228
MS.gene047951.t1 MTR_4g105630 40.893 291 169 2 95 383 81 370 2.30e-66 216
MS.gene047951.t1 MTR_3g077640 39.017 346 194 4 47 390 72 402 2.17e-65 214
MS.gene047951.t1 MTR_4g088350 41.418 268 148 4 126 389 15 277 9.30e-63 204
MS.gene047951.t1 MTR_4g105630 37.759 241 147 2 95 333 81 320 8.65e-45 159
MS.gene047951.t1 MTR_4g105630 39.535 215 128 1 95 307 81 295 2.11e-42 152
MS.gene047951.t1 MTR_1g030320 26.557 305 185 7 106 390 21 306 1.72e-23 100
MS.gene047951.t1 MTR_3g072380 26.230 305 186 7 106 390 22 307 2.52e-23 100
MS.gene047951.t1 MTR_3g070000 23.548 310 222 10 91 391 17 320 4.24e-17 82.0
MS.gene047951.t1 MTR_3g072380 30.357 168 107 3 106 266 22 186 1.02e-16 79.3
MS.gene047951.t1 MTR_5g081710 23.649 296 213 8 104 391 14 304 1.65e-15 77.0
MS.gene047951.t1 MTR_4g018760 23.129 294 215 6 105 391 19 308 4.03e-15 76.3
MS.gene047951.t1 MTR_3g462070 21.843 293 218 6 106 391 17 305 2.91e-14 73.6
MS.gene047951.t1 MTR_4g101860 22.442 303 214 6 101 391 55 348 8.67e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047951.t1 AT1G61800 80.435 368 63 2 24 390 29 388 0.0 592
MS.gene047951.t1 AT1G61800 79.404 369 67 2 24 391 29 389 0.0 586
MS.gene047951.t1 AT5G54800 79.379 354 60 3 41 389 42 387 0.0 547
MS.gene047951.t1 AT5G17630 47.042 355 182 5 37 388 51 402 4.74e-109 327
MS.gene047951.t1 AT4G03950 57.288 295 65 6 99 390 18 254 2.85e-93 282
MS.gene047951.t1 AT5G46110 42.208 308 169 4 97 400 106 408 2.17e-78 248
MS.gene047951.t1 AT5G46110 43.098 297 160 4 97 389 106 397 1.60e-77 246
MS.gene047951.t1 AT5G46110 42.525 301 161 5 106 402 2 294 4.50e-77 241
MS.gene047951.t1 AT5G46110 41.751 297 153 5 97 389 106 386 2.55e-72 232
MS.gene047951.t1 AT3G01550 40.717 307 179 2 87 390 67 373 5.53e-68 221
MS.gene047951.t1 AT3G01550 39.739 307 177 3 87 390 67 368 7.14e-64 210
MS.gene047951.t1 AT5G33320 41.017 295 171 2 97 389 106 399 4.55e-62 206
MS.gene047951.t1 AT1G77610 25.503 298 197 5 106 390 16 301 9.02e-24 101
MS.gene047951.t1 AT1G21870 25.503 298 197 6 106 390 22 307 8.04e-23 99.0
MS.gene047951.t1 AT5G05820 24.252 301 214 9 99 391 12 306 4.34e-18 84.7
MS.gene047951.t1 AT3G11320 23.588 301 216 9 99 391 12 306 3.54e-16 79.3
MS.gene047951.t1 AT1G12500 23.986 296 212 8 104 391 64 354 2.36e-13 71.2
MS.gene047951.t1 AT5G04160 22.115 312 230 7 88 391 1 307 7.46e-11 63.2

Find 76 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGTCACATTCTTTCTTT+TGG 0.109741 2.4:+64690920 None:intergenic
CTAAAGTTAACTTGGATTTC+TGG 0.136067 2.4:-64690647 MS.gene047951:CDS
AATTCTTGTTTCAGGATTTA+TGG 0.170342 2.4:-64689931 MS.gene047951:intron
CTCTCATTGTTGCCAATAAT+TGG 0.171795 2.4:-64690069 MS.gene047951:CDS
CAAATTTGGCATTTGTATTC+AGG 0.181063 2.4:-64689896 MS.gene047951:CDS
CAGAAATCCAAGTTAACTTT+AGG 0.181603 2.4:+64690648 None:intergenic
GGGAGCTATGATATCAAATT+TGG 0.191205 2.4:-64689910 MS.gene047951:CDS
GGAGCAGCTATTGCAATTCT+TGG 0.270973 2.4:-64689384 MS.gene047951:CDS
ATGACACTTGATTATATAAA+TGG 0.296751 2.4:+64689529 None:intergenic
TGAAAGATAGAGAGGTTTCT+TGG 0.315407 2.4:+64690974 None:intergenic
AGTGCGCAACCACCAATTAT+TGG 0.320937 2.4:+64690057 None:intergenic
TGTTAATCTCATGGGCTACT+AGG 0.323213 2.4:-64690683 MS.gene047951:CDS
CAACCAGCAGTCCAAACTTT+AGG 0.328755 2.4:+64689756 None:intergenic
GACTCACGGTTGGAGCTCTA+AGG 0.331327 2.4:-64689117 MS.gene047951:CDS
ATTTCCTATGCTAAATGTTA+AGG 0.334042 2.4:+64689482 None:intergenic
ATTCTTGTTTCAGGATTTAT+GGG 0.368019 2.4:-64689930 MS.gene047951:intron
TATGCTTACCAGACAAAATT+CGG 0.368126 2.4:+64689708 None:intergenic
CTGTGGAGGGTCCTAAAGTT+TGG 0.369475 2.4:-64689767 MS.gene047951:CDS
CTCTCATTATGTTAATCTCA+TGG 0.373482 2.4:-64690692 MS.gene047951:CDS
AGAAATCCAAGTTAACTTTA+GGG 0.384705 2.4:+64690649 None:intergenic
GAACTCAATTTCAATATGAT+TGG 0.386036 2.4:-64690021 MS.gene047951:intron
CAAACAGCTGTGTCTCAAAT+TGG 0.392629 2.4:-64689732 MS.gene047951:CDS
AGCTGCTCCAAGAGCATTGA+TGG 0.393145 2.4:+64689398 None:intergenic
GGCAACAATGAGAGGTAAAC+TGG 0.394912 2.4:+64690078 None:intergenic
AGTGAATGAAACTGCTACTT+TGG 0.405993 2.4:+64690176 None:intergenic
TTTCCTATGCTAAATGTTAA+GGG 0.408792 2.4:+64689483 None:intergenic
GGTCCTAAAGTTTGGACTGC+TGG 0.426259 2.4:-64689759 MS.gene047951:CDS
GCAGCTCAAAAGCTCAAGAT+TGG 0.427817 2.4:-64690831 MS.gene047951:CDS
TCCACTTTATCCTTAGCTGC+TGG 0.430126 2.4:-64690717 MS.gene047951:CDS
TTCTCAAAGAAAGGAATGAA+TGG 0.434361 2.4:-64689867 MS.gene047951:CDS
AGAATTGAAAATTGAGACCT+TGG 0.444612 2.4:+64691047 None:intergenic
CTTCAACCCATCAATGCTCT+TGG 0.453683 2.4:-64689405 MS.gene047951:CDS
GTAGCTGTGGCACACACAAT+TGG 0.470268 2.4:-64690225 MS.gene047951:CDS
ACCAGCAGCTAAGGATAAAG+TGG 0.472955 2.4:+64690716 None:intergenic
GTTAATCTCATGGGCTACTA+GGG 0.473011 2.4:-64690682 MS.gene047951:CDS
TGAGGCCCCTAAAGTTAACT+TGG 0.473088 2.4:-64690655 MS.gene047951:CDS
TAGCTGTGGCACACACAATT+GGG 0.496344 2.4:-64690224 MS.gene047951:CDS
AGGAATATATTCTCAAAGAA+AGG 0.498153 2.4:-64689876 MS.gene047951:CDS
GAAAATTGAGACCTTGGAAG+TGG 0.499489 2.4:+64691053 None:intergenic
ATGAATGGGATGAGTGTTAG+TGG 0.502849 2.4:-64689852 MS.gene047951:CDS
TGGCACCTTCATCTATTCAC+AGG 0.508331 2.4:-64689364 MS.gene047951:intron
TCTCAAAGAAAGGAATGAAT+GGG 0.514111 2.4:-64689866 MS.gene047951:CDS
TGAGTGGATGAAAGATAGAG+AGG 0.518129 2.4:+64690966 None:intergenic
CCCTCCACAGCAATAGCAAA+AGG 0.519923 2.4:+64689780 None:intergenic
CAGCTCAAAAGCTCAAGATT+GGG 0.521252 2.4:-64690830 MS.gene047951:CDS
TCTCATTATGTTAATCTCAT+GGG 0.522285 2.4:-64690691 MS.gene047951:CDS
AATGAGAGAACCAGCAGCTA+AGG 0.537711 2.4:+64690707 None:intergenic
TCAAAATGTTGAACAAAACA+TGG 0.542137 2.4:-64691015 MS.gene047951:CDS
TCATTGTTGCCAATAATTGG+TGG 0.544739 2.4:-64690066 MS.gene047951:CDS
AACATTGAACTTCCTAGTGA+AGG 0.545080 2.4:-64690855 MS.gene047951:CDS
CTAGTATCAAGATTCATGCT+TGG 0.552737 2.4:-64690117 MS.gene047951:CDS
CTAAAGTTTGGACTGCTGGT+TGG 0.555262 2.4:-64689755 MS.gene047951:CDS
CATCATCAAGAGTGGAGAGC+CGG 0.558982 2.4:-64690151 MS.gene047951:CDS
GACAGAATGTCAAGCTTATG+AGG 0.561271 2.4:-64690904 MS.gene047951:CDS
GCACAAAGTTTCCACTTCCA+AGG 0.563876 2.4:-64691064 MS.gene047951:CDS
GAAATCCAAGTTAACTTTAG+GGG 0.565634 2.4:+64690650 None:intergenic
CTTATGAGGCTGATAGATCA+AGG 0.566563 2.4:-64690890 MS.gene047951:CDS
TCTCCCTTAACATTTAGCAT+AGG 0.566878 2.4:-64689486 MS.gene047951:CDS
GTGGAAGTGAGCCAAGGGTA+AGG 0.571201 2.4:+64690735 None:intergenic
GATACTAGCACACTGAAAGC+CGG 0.571541 2.4:+64690132 None:intergenic
TGGGAACGATGACTCACGGT+TGG 0.581791 2.4:-64689127 MS.gene047951:CDS
ATGGGCTACTAGGGTTGCTG+AGG 0.583408 2.4:-64690673 MS.gene047951:CDS
ATTGGGTATGCAGGTAGCTG+TGG 0.585242 2.4:-64690238 MS.gene047951:intron
ATTCACCTGTGAATAGATGA+AGG 0.585857 2.4:+64689359 None:intergenic
ACTCACGGTTGGAGCTCTAA+GGG 0.589290 2.4:-64689116 MS.gene047951:CDS
TTCACTCACATCATCAAGAG+TGG 0.593759 2.4:-64690159 MS.gene047951:CDS
TGGTGTGTGAAAGATAATGA+TGG 0.599746 2.4:+64689428 None:intergenic
CGCATGGGAACGATGACTCA+CGG 0.599817 2.4:-64689131 MS.gene047951:CDS
AGTTCAATGTTAATCTCTAG+TGG 0.602873 2.4:+64690867 None:intergenic
CAATTATTGGCAACAATGAG+AGG 0.616851 2.4:+64690070 None:intergenic
GATAAAGTGGAAGTGAGCCA+AGG 0.631302 2.4:+64690729 None:intergenic
CAAAATGTTGAACAAAACAT+GGG 0.645558 2.4:-64691014 MS.gene047951:CDS
GCTGCTCCAAGAGCATTGAT+GGG 0.645674 2.4:+64689399 None:intergenic
GAGCAGGCTAAACAGAATTG+CGG 0.647389 2.4:-64689173 MS.gene047951:intron
ATAAAGTGGAAGTGAGCCAA+GGG 0.650767 2.4:+64690730 None:intergenic
AGAGCATTGATGGGTTGAAG+TGG 0.671770 2.4:+64689408 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTTTAATTAATAATAATTT+GGG - chr2.4:64690611-64690630 MS.gene047951:intron 10.0%
!! TTTATTACTTAAATTATTAC+AGG - chr2.4:64689846-64689865 MS.gene047951:CDS 10.0%
!!! TTTTTTTTTTTTATGTTTAC+AGG - chr2.4:64690635-64690654 MS.gene047951:CDS 10.0%
!! ATCTTTATAGTTTATACTTT+GGG - chr2.4:64690905-64690924 MS.gene047951:CDS 15.0%
!! CGCTTTAATTAATAATAATT+TGG - chr2.4:64690610-64690629 MS.gene047951:intron 15.0%
!! AATTAGAATGAAAAGAAAGT+GGG + chr2.4:64689621-64689640 None:intergenic 20.0%
!! ACCTAAGAAATTTCAAAATT+GGG - chr2.4:64689958-64689977 MS.gene047951:intron 20.0%
!! ATGACACTTGATTATATAAA+TGG + chr2.4:64690687-64690706 None:intergenic 20.0%
!! GATCTTTATAGTTTATACTT+TGG - chr2.4:64690904-64690923 MS.gene047951:CDS 20.0%
!! TATACTAGTTCAAATTTCTT+CGG - chr2.4:64690584-64690603 MS.gene047951:intron 20.0%
!!! TTTTTTTTATGTTTACAGGT+GGG - chr2.4:64690639-64690658 MS.gene047951:CDS 20.0%
!!! TTTTTTTTTATGTTTACAGG+TGG - chr2.4:64690638-64690657 MS.gene047951:CDS 20.0%
! AGAAATCCAAGTTAACTTTA+GGG + chr2.4:64689567-64689586 None:intergenic 25.0%
! AGGAATATATTCTCAAAGAA+AGG - chr2.4:64690337-64690356 MS.gene047951:intron 25.0%
! ATTAGAATGAAAAGAAAGTG+GGG + chr2.4:64689620-64689639 None:intergenic 25.0%
! ATTTCCTATGCTAAATGTTA+AGG + chr2.4:64690734-64690753 None:intergenic 25.0%
! CAAAATGTTGAACAAAACAT+GGG - chr2.4:64689199-64689218 MS.gene047951:intron 25.0%
! CAATTAGAATGAAAAGAAAG+TGG + chr2.4:64689622-64689641 None:intergenic 25.0%
! CACCTAAGAAATTTCAAAAT+TGG - chr2.4:64689957-64689976 MS.gene047951:intron 25.0%
! GAACTCAATTTCAATATGAT+TGG - chr2.4:64690192-64690211 MS.gene047951:CDS 25.0%
! GATCAAGTACATAATTTAGT+AGG + chr2.4:64690888-64690907 None:intergenic 25.0%
! TCAAAATGTTGAACAAAACA+TGG - chr2.4:64689198-64689217 MS.gene047951:intron 25.0%
! TCTCATTATGTTAATCTCAT+GGG - chr2.4:64689522-64689541 MS.gene047951:CDS 25.0%
! TTTCCTATGCTAAATGTTAA+GGG + chr2.4:64690733-64690752 None:intergenic 25.0%
!! AATTCTTGTTTCAGGATTTA+TGG - chr2.4:64690282-64690301 MS.gene047951:intron 25.0%
!! ATTCTTGTTTCAGGATTTAT+GGG - chr2.4:64690283-64690302 MS.gene047951:intron 25.0%
!! GATTTTTCTTACAGGAAAAA+GGG + chr2.4:64689596-64689615 None:intergenic 25.0%
!! TACAATATCAATGCTCTAAA+TGG - chr2.4:64690256-64690275 MS.gene047951:intron 25.0%
!! TGATTTTTCTTACAGGAAAA+AGG + chr2.4:64689597-64689616 None:intergenic 25.0%
!!! ACCCAATTTTGAAATTTCTT+AGG + chr2.4:64689962-64689981 None:intergenic 25.0%
!!! GTTTTGTTCAACATTTTGAA+TGG + chr2.4:64689197-64689216 None:intergenic 25.0%
AATTTCAAAATTGGGTATGC+AGG - chr2.4:64689966-64689985 MS.gene047951:intron 30.0%
AGAATTGAAAATTGAGACCT+TGG + chr2.4:64689169-64689188 None:intergenic 30.0%
AGTTCAATGTTAATCTCTAG+TGG + chr2.4:64689349-64689368 None:intergenic 30.0%
CAAATTTGGCATTTGTATTC+AGG - chr2.4:64690317-64690336 MS.gene047951:intron 30.0%
CAGAAATCCAAGTTAACTTT+AGG + chr2.4:64689568-64689587 None:intergenic 30.0%
CTCTCATTATGTTAATCTCA+TGG - chr2.4:64689521-64689540 MS.gene047951:CDS 30.0%
GAAATCCAAGTTAACTTTAG+GGG + chr2.4:64689566-64689585 None:intergenic 30.0%
GAGATTGAAACATTATGTGA+GGG - chr2.4:64690978-64690997 MS.gene047951:CDS 30.0%
TATGCTTACCAGACAAAATT+CGG + chr2.4:64690508-64690527 None:intergenic 30.0%
TCTCAAAGAAAGGAATGAAT+GGG - chr2.4:64690347-64690366 MS.gene047951:intron 30.0%
TGAGATTGAAACATTATGTG+AGG - chr2.4:64690977-64690996 MS.gene047951:CDS 30.0%
TTCTCAAAGAAAGGAATGAA+TGG - chr2.4:64690346-64690365 MS.gene047951:intron 30.0%
TTGAATGTAACTACATGCAT+CGG + chr2.4:64689692-64689711 None:intergenic 30.0%
! AATGGATCAATTCTTGTTTC+AGG - chr2.4:64690274-64690293 MS.gene047951:intron 30.0%
! CTAAAGTTAACTTGGATTTC+TGG - chr2.4:64689566-64689585 MS.gene047951:intron 30.0%
! TCTGTCACATTCTTTCTTTT+GGG + chr2.4:64689295-64689314 None:intergenic 30.0%
! TGTTCAACATTTTGAATGGT+TGG + chr2.4:64689193-64689212 None:intergenic 30.0%
! TTCTGTCACATTCTTTCTTT+TGG + chr2.4:64689296-64689315 None:intergenic 30.0%
!!! TGTTGATTTTTTGAAAGAGC+AGG - chr2.4:64691024-64691043 MS.gene047951:CDS 30.0%
AACATTGAACTTCCTAGTGA+AGG - chr2.4:64689358-64689377 MS.gene047951:intron 35.0%
ATTCACCTGTGAATAGATGA+AGG + chr2.4:64690857-64690876 None:intergenic 35.0%
CAATTATTGGCAACAATGAG+AGG + chr2.4:64690146-64690165 None:intergenic 35.0%
CTAGTATCAAGATTCATGCT+TGG - chr2.4:64690096-64690115 MS.gene047951:CDS 35.0%
CTCTCATTGTTGCCAATAAT+TGG - chr2.4:64690144-64690163 MS.gene047951:CDS 35.0%
GGGAGCTATGATATCAAATT+TGG - chr2.4:64690303-64690322 MS.gene047951:intron 35.0%
GTAACTACATTTGTCAGTGT+CGG + chr2.4:64689816-64689835 None:intergenic 35.0%
GTGAGCACTAAAAATAACAC+CGG + chr2.4:64689940-64689959 None:intergenic 35.0%
TCATTGTTGCCAATAATTGG+TGG - chr2.4:64690147-64690166 MS.gene047951:CDS 35.0%
TCTCCCTTAACATTTAGCAT+AGG - chr2.4:64690727-64690746 MS.gene047951:CDS 35.0%
TGAAAGATAGAGAGGTTTCT+TGG + chr2.4:64689242-64689261 None:intergenic 35.0%
TGGTGTGTGAAAGATAATGA+TGG + chr2.4:64690788-64690807 None:intergenic 35.0%
! AAATTGGTCCGAATTTTGTC+TGG - chr2.4:64690497-64690516 MS.gene047951:intron 35.0%
! AGTGAATGAAACTGCTACTT+TGG + chr2.4:64690040-64690059 None:intergenic 35.0%
! GAGCAATGCAAAATTTTGAG+TGG + chr2.4:64689266-64689285 None:intergenic 35.0%
! TTTTTATGTTTACAGGTGGG+TGG - chr2.4:64690642-64690661 MS.gene047951:CDS 35.0%
!! GTTGTATTTTGCTACTTGGT+GGG - chr2.4:64689405-64689424 MS.gene047951:CDS 35.0%
!! TTGGGTTGTATTTTGCTACT+TGG - chr2.4:64689401-64689420 MS.gene047951:CDS 35.0%
!!! ACTTTTTCAACCTGAAGTGT+CGG + chr2.4:64689784-64689803 None:intergenic 35.0%
ATAAAGTGGAAGTGAGCCAA+GGG + chr2.4:64689486-64689505 None:intergenic 40.0%
ATGAATGGGATGAGTGTTAG+TGG - chr2.4:64690361-64690380 MS.gene047951:intron 40.0%
CAAACAGCTGTGTCTCAAAT+TGG - chr2.4:64690481-64690500 MS.gene047951:intron 40.0%
CAATGTGTCAGTGTAATGTC+CGG - chr2.4:64689918-64689937 MS.gene047951:CDS 40.0%
CAGCTCAAAAGCTCAAGATT+GGG - chr2.4:64689383-64689402 MS.gene047951:CDS 40.0%
CTATGTGTCAGTATAGTGTC+TGG - chr2.4:64689872-64689891 MS.gene047951:CDS 40.0%
CTTATGAGGCTGATAGATCA+AGG - chr2.4:64689323-64689342 MS.gene047951:intron 40.0%
GAAAATTGAGACCTTGGAAG+TGG + chr2.4:64689163-64689182 None:intergenic 40.0%
GACACATTGATATCAGTCAC+TGG + chr2.4:64689907-64689926 None:intergenic 40.0%
GACAGAATGTCAAGCTTATG+AGG - chr2.4:64689309-64689328 MS.gene047951:intron 40.0%
GTACTGTTGAAAAGTGGCAT+CGG + chr2.4:64689762-64689781 None:intergenic 40.0%
TGAGTGGATGAAAGATAGAG+AGG + chr2.4:64689250-64689269 None:intergenic 40.0%
TTCACTCACATCATCAAGAG+TGG - chr2.4:64690054-64690073 MS.gene047951:CDS 40.0%
! AGTGGGGTGATTTTTCTTAC+AGG + chr2.4:64689604-64689623 None:intergenic 40.0%
! GAAGCTTTTATCGAACGCAT+GGG - chr2.4:64691067-64691086 MS.gene047951:CDS 40.0%
! GTTAATCTCATGGGCTACTA+GGG - chr2.4:64689531-64689550 MS.gene047951:CDS 40.0%
! GTTTTCACTTTCACTAGCAC+TGG - chr2.4:64689641-64689660 MS.gene047951:intron 40.0%
! TGAAGCTTTTATCGAACGCA+TGG - chr2.4:64691066-64691085 MS.gene047951:CDS 40.0%
! TGAATGCTTTTCCTTACCCT+TGG - chr2.4:64689467-64689486 MS.gene047951:CDS 40.0%
! TGTTAATCTCATGGGCTACT+AGG - chr2.4:64689530-64689549 MS.gene047951:CDS 40.0%
!! GGTTGTATTTTGCTACTTGG+TGG - chr2.4:64689404-64689423 MS.gene047951:CDS 40.0%
AATGAGAGAACCAGCAGCTA+AGG + chr2.4:64689509-64689528 None:intergenic 45.0%
ACCAGCAGCTAAGGATAAAG+TGG + chr2.4:64689500-64689519 None:intergenic 45.0%
AGTGCGCAACCACCAATTAT+TGG + chr2.4:64690159-64690178 None:intergenic 45.0%
CAACCAGCAGTCCAAACTTT+AGG + chr2.4:64690460-64690479 None:intergenic 45.0%
CTAAAGTTTGGACTGCTGGT+TGG - chr2.4:64690458-64690477 MS.gene047951:intron 45.0%
CTTCAACCCATCAATGCTCT+TGG - chr2.4:64690808-64690827 MS.gene047951:CDS 45.0%
GAGCAGGCTAAACAGAATTG+CGG - chr2.4:64691040-64691059 MS.gene047951:CDS 45.0%
GATAAAGTGGAAGTGAGCCA+AGG + chr2.4:64689487-64689506 None:intergenic 45.0%
GATACTAGCACACTGAAAGC+CGG + chr2.4:64690084-64690103 None:intergenic 45.0%
GCACAAAGTTTCCACTTCCA+AGG - chr2.4:64689149-64689168 MS.gene047951:CDS 45.0%
GCAGCTCAAAAGCTCAAGAT+TGG - chr2.4:64689382-64689401 MS.gene047951:CDS 45.0%
GGAGCAGCTATTGCAATTCT+TGG - chr2.4:64690829-64690848 MS.gene047951:CDS 45.0%
GGCAACAATGAGAGGTAAAC+TGG + chr2.4:64690138-64690157 None:intergenic 45.0%
TAGCTGTGGCACACACAATT+GGG - chr2.4:64689989-64690008 MS.gene047951:intron 45.0%
TCCACTTTATCCTTAGCTGC+TGG - chr2.4:64689496-64689515 MS.gene047951:CDS 45.0%
TGAGGCCCCTAAAGTTAACT+TGG - chr2.4:64689558-64689577 MS.gene047951:CDS 45.0%
TGGCACCTTCATCTATTCAC+AGG - chr2.4:64690849-64690868 MS.gene047951:CDS 45.0%
! GTGTCGGTACTGTTGAAAAG+TGG + chr2.4:64689768-64689787 None:intergenic 45.0%
! TTTTGAGCTGCTCCTTCACT+AGG + chr2.4:64689373-64689392 None:intergenic 45.0%
!! AGAGCATTGATGGGTTGAAG+TGG + chr2.4:64690808-64690827 None:intergenic 45.0%
!! TTCAACAGTACCGACACTTC+AGG - chr2.4:64689771-64689790 MS.gene047951:CDS 45.0%
!!! ACCTTTTGCTATTGCTGTGG+AGG - chr2.4:64690432-64690451 MS.gene047951:intron 45.0%
!!! CACACCTTTTGCTATTGCTG+TGG - chr2.4:64690429-64690448 MS.gene047951:intron 45.0%
!!! CCTTTTGCTATTGCTGTGGA+GGG - chr2.4:64690433-64690452 MS.gene047951:intron 45.0%
ATTGGGTATGCAGGTAGCTG+TGG - chr2.4:64689975-64689994 MS.gene047951:intron 50.0%
CATCATCAAGAGTGGAGAGC+CGG - chr2.4:64690062-64690081 MS.gene047951:CDS 50.0%
CCCTCCACAGCAATAGCAAA+AGG + chr2.4:64690436-64690455 None:intergenic 50.0%
CTGTGGAGGGTCCTAAAGTT+TGG - chr2.4:64690446-64690465 MS.gene047951:intron 50.0%
GGTCCTAAAGTTTGGACTGC+TGG - chr2.4:64690454-64690473 MS.gene047951:intron 50.0%
GTAGCTGTGGCACACACAAT+TGG - chr2.4:64689988-64690007 MS.gene047951:intron 50.0%
! ACTCACGGTTGGAGCTCTAA+GGG - chr2.4:64691097-64691116 MS.gene047951:CDS 50.0%
!! AGCTGCTCCAAGAGCATTGA+TGG + chr2.4:64690818-64690837 None:intergenic 50.0%
!! GCTGCTCCAAGAGCATTGAT+GGG + chr2.4:64690817-64690836 None:intergenic 50.0%
CGCATGGGAACGATGACTCA+CGG - chr2.4:64691082-64691101 MS.gene047951:CDS 55.0%
GACTCACGGTTGGAGCTCTA+AGG - chr2.4:64691096-64691115 MS.gene047951:CDS 55.0%
GTGGAAGTGAGCCAAGGGTA+AGG + chr2.4:64689481-64689500 None:intergenic 55.0%
TGGGAACGATGACTCACGGT+TGG - chr2.4:64691086-64691105 MS.gene047951:CDS 55.0%
! ATGGGCTACTAGGGTTGCTG+AGG - chr2.4:64689540-64689559 MS.gene047951:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 64689106 64691129 64689106 ID=MS.gene047951
chr2.4 mRNA 64689106 64691129 64689106 ID=MS.gene047951.t1;Parent=MS.gene047951
chr2.4 exon 64690632 64691129 64690632 ID=MS.gene047951.t1.exon1;Parent=MS.gene047951.t1
chr2.4 CDS 64690632 64691129 64690632 ID=cds.MS.gene047951.t1;Parent=MS.gene047951.t1
chr2.4 exon 64690022 64690247 64690022 ID=MS.gene047951.t1.exon2;Parent=MS.gene047951.t1
chr2.4 CDS 64690022 64690247 64690022 ID=cds.MS.gene047951.t1;Parent=MS.gene047951.t1
chr2.4 exon 64689717 64689939 64689717 ID=MS.gene047951.t1.exon3;Parent=MS.gene047951.t1
chr2.4 CDS 64689717 64689939 64689717 ID=cds.MS.gene047951.t1;Parent=MS.gene047951.t1
chr2.4 exon 64689365 64689578 64689365 ID=MS.gene047951.t1.exon4;Parent=MS.gene047951.t1
chr2.4 CDS 64689365 64689578 64689365 ID=cds.MS.gene047951.t1;Parent=MS.gene047951.t1
chr2.4 exon 64689106 64689189 64689106 ID=MS.gene047951.t1.exon5;Parent=MS.gene047951.t1
chr2.4 CDS 64689106 64689189 64689106 ID=cds.MS.gene047951.t1;Parent=MS.gene047951.t1
Gene Sequence

>MS.gene047951

ATGATCTCTTCAATCAAGTGCACAAGTACATCATCACTTACAAGCACAAAGTTTCCACTTCCAAGGTCTCAATTTTCAATTCTACCAACCATTCAAAATGTTGAACAAAACATGGGTCTCTCTCTACTTTCTTCCAAGAAACCTCTCTATCTTTCATCCACTCAAAATTTTGCATTGCTCACAAAACCCAAAAGAAAGAATGTGACAGAATGTCAAGCTTATGAGGCTGATAGATCAAGGCCACTAGAGATTAACATTGAACTTCCTAGTGAAGGAGCAGCTCAAAAGCTCAAGATTGGGTTGTATTTTGCTACTTGGTGGGCTTTGAATGTTGTTTTCAACATATACAACAAGAAAGTTTTGAATGCTTTTCCTTACCCTTGGCTCACTTCCACTTTATCCTTAGCTGCTGGTTCTCTCATTATGTTAATCTCATGGGCTACTAGGGTTGCTGAGGCCCCTAAAGTTAACTTGGATTTCTGGAAAGCCCTTTTTCCTGTAAGAAAAATCACCCCACTTTCTTTTCATTCTAATTGTTTTCACTTTCACTAGCACTGGCATAAGACACATTTAGTGTCTGACACCGATGCATGTAGTTACATTCAATTACTTTCATTTCTAAATTATTATCAGTGTATGTCTCAGTGTCGTATCCGATGCCACTTTTCAACAGTACCGACACTTCAGGTTGAAAAAGTGTCTAATGTCCGACACTGACAAATGTAGTTACATTCAATTAGTTTATTACTTAAATTATTACAGGTATCTATGTGTCAGTATAGTGTCTGGTTTCTATCTCCAGTGACTGATATCAATGTGTCAGTGTAATGTCCGGTGTTATTTTTAGTGCTCACCTAAGAAATTTCAAAATTGGGTATGCAGGTAGCTGTGGCACACACAATTGGGCATGTTGCTGCAACTGTGAGTATGTCCAAAGTAGCAGTTTCATTCACTCACATCATCAAGAGTGGAGAGCCGGCTTTCAGTGTGCTAGTATCAAGATTCATGCTTGGAGAAGCATTCGCTATGCCAGTTTACCTCTCATTGTTGCCAATAATTGGTGGTTGCGCACTAGCTGCTATGACAGAACTCAATTTCAATATGATTGGTAAGAACAACATAACAAACAATTATTCACATAAATATAAAGTACAATATCAATGCTCTAAATGGATCAATTCTTGTTTCAGGATTTATGGGAGCTATGATATCAAATTTGGCATTTGTATTCAGGAATATATTCTCAAAGAAAGGAATGAATGGGATGAGTGTTAGTGGAATGAACTATTATGCTTGTCTTTCAATGTTATCTCTTTTAATTCTCACACCTTTTGCTATTGCTGTGGAGGGTCCTAAAGTTTGGACTGCTGGTTGGCAAACAGCTGTGTCTCAAATTGGTCCGAATTTTGTCTGGTAAGCATAAAACTCTACGAATTTATGTGTCAGTTCAGTGATAAAAGTTTGATTTTCTAAAGTGATATACTAGTTCAAATTTCTTCGGAAACGCTTTAATTAATAATAATTTGGGATTTTTTTTTTTTATGTTTACAGGTGGGTGGTTGCACAAAGTGTGTTCTACCATTTATATAATCAAGTGTCATACATGTCTCTTGATCAGATTTCTCCCTTAACATTTAGCATAGGAAATACAATGAAGAGAATATCTGTAATAGTCTCTTCCATCATTATCTTTCACACACCACTTCAACCCATCAATGCTCTTGGAGCAGCTATTGCAATTCTTGGCACCTTCATCTATTCACAGGTGAATTCTCATTTCCTACTAAATTATGTACTTGATCTTTATAGTTTATACTTTGGGAATGTTCTTTTTATGTGTGTGTGTGTGTGTGTGTGTTTAATATCTTGTTTGAGATTGAAACATTATGTGAGGGATGCAATTAATTAACTCAATTTCTGTTGATTTTTTGAAAGAGCAGGCTAAACAGAATTGCGGTTTTGAAGCTTTTATCGAACGCATGGGAACGATGACTCACGGTTGGAGCTCTAAGGGTATACTATGA
Protein sequence

>MS.gene047951.t1

MISSIKCTSTSSLTSTKFPLPRSQFSILPTIQNVEQNMGLSLLSSKKPLYLSSTQNFALLTKPKRKNVTECQAYEADRSRPLEINIELPSEGAAQKLKIGLYFATWWALNVVFNIYNKKVLNAFPYPWLTSTLSLAAGSLIMLISWATRVAEAPKVNLDFWKALFPVAVAHTIGHVAATVSMSKVAVSFTHIIKSGEPAFSVLVSRFMLGEAFAMPVYLSLLPIIGGCALAAMTELNFNMIGFMGAMISNLAFVFRNIFSKKGMNGMSVSGMNYYACLSMLSLLILTPFAIAVEGPKVWTAGWQTAVSQIGPNFVWWVVAQSVFYHLYNQVSYMSLDQISPLTFSIGNTMKRISVIVSSIIIFHTPLQPINALGAAIAILGTFIYSQAKQNCGFEAFIERMGTMTHGWSSKGIL