AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74587


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74587.t1 MTR_2g029180 100.000 263 0 0 1 263 146 408 0.0 526
MS.gene74587.t1 MTR_3g049400 55.200 250 107 4 1 248 153 399 4.31e-82 252
MS.gene74587.t1 MTR_2g022700 54.800 250 108 4 1 248 147 393 1.65e-81 250
MS.gene74587.t1 MTR_4g131800 50.741 270 109 5 1 247 144 412 2.86e-67 225
MS.gene74587.t1 MTR_4g105630 44.048 252 135 4 4 252 131 379 5.31e-59 192
MS.gene74587.t1 MTR_6g005480 46.154 260 135 4 3 258 158 416 1.06e-58 192
MS.gene74587.t1 MTR_4g088350 42.045 264 138 6 2 261 32 284 1.38e-57 186
MS.gene74587.t1 MTR_5g044220 40.625 256 142 5 5 256 151 400 2.55e-56 186
MS.gene74587.t1 MTR_4g088350 42.045 264 138 6 2 261 143 395 5.07e-56 185
MS.gene74587.t1 MTR_4g105630 43.210 243 132 4 4 243 131 370 5.31e-55 181
MS.gene74587.t1 MTR_3g077640 44.531 256 136 4 4 256 156 408 4.55e-54 180
MS.gene74587.t1 MTR_4g105630 40.816 196 110 4 4 196 131 323 1.32e-34 127
MS.gene74587.t1 MTR_4g105630 41.143 175 95 3 4 170 131 305 1.17e-31 119
MS.gene74587.t1 MTR_3g072380 29.046 241 164 4 7 245 67 302 1.01e-19 87.4
MS.gene74587.t1 MTR_1g030320 28.099 242 165 6 7 245 66 301 1.16e-17 81.6
MS.gene74587.t1 MTR_3g070000 24.800 250 181 5 7 253 77 322 9.61e-15 73.2
MS.gene74587.t1 MTR_4g101860 28.571 217 147 4 40 253 139 350 1.24e-14 73.2
MS.gene74587.t1 MTR_4g018760 26.383 235 167 4 16 248 75 305 1.60e-14 72.4
MS.gene74587.t1 MTR_5g081710 25.806 217 155 4 39 253 94 306 1.37e-13 69.7
MS.gene74587.t1 MTR_3g462070 24.434 221 157 6 39 256 95 308 1.18e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74587.t1 AT5G17630 83.650 263 43 0 1 263 155 417 2.07e-160 452
MS.gene74587.t1 AT5G54800 55.200 250 107 4 1 248 140 386 2.88e-82 252
MS.gene74587.t1 AT1G61800 52.800 250 113 4 1 248 140 386 1.86e-80 247
MS.gene74587.t1 AT1G61800 52.610 249 113 4 1 247 140 385 2.10e-79 245
MS.gene74587.t1 AT5G46110 39.623 265 150 5 2 262 39 297 1.15e-57 186
MS.gene74587.t1 AT5G46110 39.623 265 150 5 2 262 152 410 1.86e-56 186
MS.gene74587.t1 AT5G46110 40.530 264 142 6 2 261 152 404 3.21e-55 183
MS.gene74587.t1 AT5G46110 38.491 265 142 6 2 262 152 399 3.85e-52 175
MS.gene74587.t1 AT3G01550 44.177 249 130 4 3 248 124 366 6.10e-52 174
MS.gene74587.t1 AT3G01550 44.177 249 135 3 3 248 124 371 6.30e-52 174
MS.gene74587.t1 AT5G33320 41.406 256 146 3 3 256 153 406 2.44e-48 165
MS.gene74587.t1 AT4G03950 36.864 236 103 7 27 257 67 261 2.18e-33 122
MS.gene74587.t1 AT1G77610 30.769 208 138 3 39 245 94 296 5.85e-20 88.2
MS.gene74587.t1 AT1G21870 27.801 241 167 4 7 245 67 302 5.37e-19 85.5
MS.gene74587.t1 AT1G12500 26.667 225 154 5 40 261 145 361 9.50e-15 73.6
MS.gene74587.t1 AT5G05820 24.603 252 179 6 7 253 63 308 5.03e-13 68.2
MS.gene74587.t1 AT3G11320 25.114 219 154 5 39 253 96 308 2.60e-12 65.9

Find 40 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATCTGCCATTGCTATTCT+TGG 0.173141 2.3:+60387436 MS.gene74587:CDS
CCATTTGGATGCTTGTTCTT+TGG 0.202656 2.3:+60386729 None:intergenic
AGTGATTGTGTCTTCGATTT+TGG 0.242143 2.3:+60387378 MS.gene74587:CDS
TTTCCAACAGAGAAAGTTAA+TGG 0.242242 2.3:-60387337 None:intergenic
AGCTTGTGTTTCATTCTCTA+AGG 0.279265 2.3:+60386844 MS.gene74587:CDS
TGGTGATAGGTATCCAATTC+AGG 0.283536 2.3:+60386937 MS.gene74587:CDS
CAAAATTTCAAAGAAGTTGA+TGG 0.305953 2.3:+60387103 MS.gene74587:CDS
GTTGATGGTTTGAACTTATA+TGG 0.310417 2.3:+60387118 MS.gene74587:CDS
CCAAGTAGTGCAAAGATAAA+AGG 0.314629 2.3:-60386788 None:intergenic
ATTGAAAGCCAAACCTGAAT+TGG 0.343853 2.3:-60386950 None:intergenic
GTTAGGCCTCTTAATGGTCT+TGG 0.367474 2.3:+60387415 MS.gene74587:CDS
TTTCTTCTGTTCTTGGTGAT+AGG 0.374490 2.3:+60386924 MS.gene74587:CDS
GATCTGCCATTGCTATTCTT+GGG 0.384636 2.3:+60387437 MS.gene74587:CDS
CCTGCTTTGTTTCACACAAT+AGG 0.391158 2.3:+60386812 MS.gene74587:CDS
TAAGAGGCCTAACCGGATTC+CGG 0.403937 2.3:-60387405 None:intergenic
ATGGATTCCAGGGTATTATA+AGG 0.414035 2.3:+60387204 MS.gene74587:CDS
GAAGGTTCTCAATGGATTCC+AGG 0.417158 2.3:+60387193 MS.gene74587:CDS
AAAGAACAACCAAGCACAAT+TGG 0.428671 2.3:-60386977 None:intergenic
ATAGGTATCCAATTCAGGTT+TGG 0.441304 2.3:+60386942 MS.gene74587:CDS
AATCCGGTTAGGCCTCTTAA+TGG 0.445202 2.3:+60387409 MS.gene74587:CDS
GAGGTATCTTTCAATGTTGG+AGG 0.458201 2.3:+60387013 MS.gene74587:CDS
AGTCCATTAACTTTCTCTGT+TGG 0.459007 2.3:+60387334 MS.gene74587:CDS
AGACCATTAAGAGGCCTAAC+CGG 0.467988 2.3:-60387412 None:intergenic
AAGAAAGCAAAGAAAATTGA+AGG 0.478846 2.3:+60387490 MS.gene74587:CDS
TTCTTTAGCTGCTGTTACTG+AGG 0.482713 2.3:+60386994 MS.gene74587:CDS
TATAAGGCGCTTGAAGCGAT+TGG 0.482837 2.3:+60387220 MS.gene74587:CDS
ATGGTTTGAACTTATATGGT+TGG 0.489171 2.3:+60387122 MS.gene74587:CDS
TGTGCACTTATTAGCAATGT+TGG 0.491248 2.3:+60387043 MS.gene74587:CDS
CTTTCAATGTTGGAGGATTG+TGG 0.498343 2.3:+60387020 MS.gene74587:CDS
ACTGAGGTATCTTTCAATGT+TGG 0.506410 2.3:+60387010 MS.gene74587:CDS
TCAATCATCTTATCAAGCAT+TGG 0.516384 2.3:+60387303 MS.gene74587:CDS
TGGTGTTCCGGAATCCGGTT+AGG 0.528399 2.3:+60387398 MS.gene74587:CDS
GCAGATCCAAGACCATTAAG+AGG 0.528834 2.3:-60387421 None:intergenic
AGAAAGCAAAGAAAATTGAA+GGG 0.581786 2.3:+60387491 MS.gene74587:CDS
AAGGTTCTCAATGGATTCCA+GGG 0.590612 2.3:+60387194 MS.gene74587:CDS
CCTATTGTGTGAAACAAAGC+AGG 0.603409 2.3:-60386812 None:intergenic
TGGAAATACAATGAAGAGAG+TGG 0.612192 2.3:+60387354 MS.gene74587:CDS
AAAAGTCCCAAGAATAGCAA+TGG 0.616189 2.3:-60387443 None:intergenic
GAAAGCAAAGAAAATTGAAG+GGG 0.622560 2.3:+60387492 MS.gene74587:CDS
TCAAGCGCCTTATAATACCC+TGG 0.633740 2.3:-60387211 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAAGGTTTTGAGATTTT+TGG - chr2.3:60386776-60386795 None:intergenic 20.0%
!!! TTCATTTATGTATCTTTTTC+CGG + chr2.3:60387156-60387175 MS.gene74587:CDS 20.0%
! AAGAAAGCAAAGAAAATTGA+AGG + chr2.3:60387490-60387509 MS.gene74587:CDS 25.0%
! AGAAAGCAAAGAAAATTGAA+GGG + chr2.3:60387491-60387510 MS.gene74587:CDS 25.0%
! CAAAATTTCAAAGAAGTTGA+TGG + chr2.3:60387103-60387122 MS.gene74587:CDS 25.0%
! GAAAATATGACAGAAAAAAC+AGG - chr2.3:60386908-60386927 None:intergenic 25.0%
!!! GACTTTTTATATTTGGGTTT+TGG + chr2.3:60387252-60387271 MS.gene74587:CDS 25.0%
ACCCAAATATAAAAAGTCGA+AGG - chr2.3:60387250-60387269 None:intergenic 30.0%
ATGGTTTGAACTTATATGGT+TGG + chr2.3:60387122-60387141 MS.gene74587:CDS 30.0%
GAAAGCAAAGAAAATTGAAG+GGG + chr2.3:60387492-60387511 MS.gene74587:CDS 30.0%
TCAATCATCTTATCAAGCAT+TGG + chr2.3:60387303-60387322 MS.gene74587:CDS 30.0%
TTTCCAACAGAGAAAGTTAA+TGG - chr2.3:60387340-60387359 None:intergenic 30.0%
! GCCTTCGACTTTTTATATTT+GGG + chr2.3:60387246-60387265 MS.gene74587:CDS 30.0%
! GTCATATTTTCTTCTGTTCT+TGG + chr2.3:60386917-60386936 MS.gene74587:CDS 30.0%
! TCAATTTTACCAATTGTGCT+TGG + chr2.3:60386968-60386987 MS.gene74587:CDS 30.0%
! TTTTTGTAGAAGGTTCTCAA+TGG + chr2.3:60387185-60387204 MS.gene74587:CDS 30.0%
!! GTTGATGGTTTGAACTTATA+TGG + chr2.3:60387118-60387137 MS.gene74587:CDS 30.0%
!!! TATATTTGGGTTTTGGTTTC+TGG + chr2.3:60387259-60387278 MS.gene74587:CDS 30.0%
AAAAGTCCCAAGAATAGCAA+TGG - chr2.3:60387446-60387465 None:intergenic 35.0%
AAAGAACAACCAAGCACAAT+TGG - chr2.3:60386980-60386999 None:intergenic 35.0%
AGCTTGTGTTTCATTCTCTA+AGG + chr2.3:60386844-60386863 MS.gene74587:CDS 35.0%
AGTCCATTAACTTTCTCTGT+TGG + chr2.3:60387334-60387353 MS.gene74587:CDS 35.0%
ATTGAAAGCCAAACCTGAAT+TGG - chr2.3:60386953-60386972 None:intergenic 35.0%
CCAAGTAGTGCAAAGATAAA+AGG - chr2.3:60386791-60386810 None:intergenic 35.0%
TGGAAATACAATGAAGAGAG+TGG + chr2.3:60387354-60387373 MS.gene74587:CDS 35.0%
TGTGCACTTATTAGCAATGT+TGG + chr2.3:60387043-60387062 MS.gene74587:CDS 35.0%
! ACTGAGGTATCTTTCAATGT+TGG + chr2.3:60387010-60387029 MS.gene74587:CDS 35.0%
! AGTGATTGTGTCTTCGATTT+TGG + chr2.3:60387378-60387397 MS.gene74587:CDS 35.0%
! ATAGGTATCCAATTCAGGTT+TGG + chr2.3:60386942-60386961 MS.gene74587:CDS 35.0%
! ATGGATTCCAGGGTATTATA+AGG + chr2.3:60387204-60387223 MS.gene74587:CDS 35.0%
! CCTTTTATCTTTGCACTACT+TGG + chr2.3:60386788-60386807 MS.gene74587:CDS 35.0%
! CGCCTTCGACTTTTTATATT+TGG + chr2.3:60387245-60387264 MS.gene74587:CDS 35.0%
! TTTCTTCTGTTCTTGGTGAT+AGG + chr2.3:60386924-60386943 MS.gene74587:CDS 35.0%
!!! GGTTTTGAGATTTTTGGACA+AGG - chr2.3:60386770-60386789 None:intergenic 35.0%
CCTATTGTGTGAAACAAAGC+AGG - chr2.3:60386815-60386834 None:intergenic 40.0%
CCTTCTACAAAAATCGCTAC+CGG - chr2.3:60387178-60387197 None:intergenic 40.0%
CTTTCAATGTTGGAGGATTG+TGG + chr2.3:60387020-60387039 MS.gene74587:CDS 40.0%
TGGTGATAGGTATCCAATTC+AGG + chr2.3:60386937-60386956 MS.gene74587:CDS 40.0%
TTCTTTAGCTGCTGTTACTG+AGG + chr2.3:60386994-60387013 MS.gene74587:CDS 40.0%
! AAGGTTCTCAATGGATTCCA+GGG + chr2.3:60387194-60387213 MS.gene74587:CDS 40.0%
! GAGGTATCTTTCAATGTTGG+AGG + chr2.3:60387013-60387032 MS.gene74587:CDS 40.0%
! GATCTGCCATTGCTATTCTT+GGG + chr2.3:60387437-60387456 MS.gene74587:CDS 40.0%
!! CCTGCTTTGTTTCACACAAT+AGG + chr2.3:60386812-60386831 MS.gene74587:CDS 40.0%
!!! GCAATGTTGGTTTTGTGTTG+AGG + chr2.3:60387056-60387075 MS.gene74587:CDS 40.0%
!!! TGTCTTCGATTTTGGTGTTC+CGG + chr2.3:60387386-60387405 MS.gene74587:CDS 40.0%
AATCCGGTTAGGCCTCTTAA+TGG + chr2.3:60387409-60387428 MS.gene74587:CDS 45.0%
AGACCATTAAGAGGCCTAAC+CGG - chr2.3:60387415-60387434 None:intergenic 45.0%
GCAGATCCAAGACCATTAAG+AGG - chr2.3:60387424-60387443 None:intergenic 45.0%
GTTAGGCCTCTTAATGGTCT+TGG + chr2.3:60387415-60387434 MS.gene74587:CDS 45.0%
TCAAGCGCCTTATAATACCC+TGG - chr2.3:60387214-60387233 None:intergenic 45.0%
! CCGGTAGCGATTTTTGTAGA+AGG + chr2.3:60387175-60387194 MS.gene74587:CDS 45.0%
! GAAGGTTCTCAATGGATTCC+AGG + chr2.3:60387193-60387212 MS.gene74587:CDS 45.0%
! GGATCTGCCATTGCTATTCT+TGG + chr2.3:60387436-60387455 MS.gene74587:CDS 45.0%
! TATAAGGCGCTTGAAGCGAT+TGG + chr2.3:60387220-60387239 MS.gene74587:CDS 45.0%
!!! GATTTTGGTGTTCCGGAATC+CGG + chr2.3:60387393-60387412 MS.gene74587:CDS 45.0%
TAAGAGGCCTAACCGGATTC+CGG - chr2.3:60387408-60387427 None:intergenic 50.0%
TGGTGTTCCGGAATCCGGTT+AGG + chr2.3:60387398-60387417 MS.gene74587:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 60386737 60387528 60386737 ID=MS.gene74587
chr2.3 mRNA 60386737 60387528 60386737 ID=MS.gene74587.t1;Parent=MS.gene74587
chr2.3 exon 60386737 60387528 60386737 ID=MS.gene74587.t1.exon1;Parent=MS.gene74587.t1
chr2.3 CDS 60386737 60387528 60386737 ID=cds.MS.gene74587.t1;Parent=MS.gene74587.t1
Gene Sequence

>MS.gene74587

ATGCTTGTTCTTTGGTCTTTAAAGCTTCAACCTTGTCCAAAAATCTCAAAACCTTTTATCTTTGCACTACTTGGACCTGCTTTGTTTCACACAATAGGTCACATTTCAGCTTGTGTTTCATTCTCTAAGGTTGCTGTGTCTTTCACACATGTTATTAAATCAGCAGAACCTGTTTTTTCTGTCATATTTTCTTCTGTTCTTGGTGATAGGTATCCAATTCAGGTTTGGCTTTCAATTTTACCAATTGTGCTTGGTTGTTCTTTAGCTGCTGTTACTGAGGTATCTTTCAATGTTGGAGGATTGTGGTGTGCACTTATTAGCAATGTTGGTTTTGTGTTGAGGAACATTTATTCGAAAAAAAGTTTACAAAATTTCAAAGAAGTTGATGGTTTGAACTTATATGGTTGGATTACTATACTTTCATTTATGTATCTTTTTCCGGTAGCGATTTTTGTAGAAGGTTCTCAATGGATTCCAGGGTATTATAAGGCGCTTGAAGCGATTGGAACGCCTTCGACTTTTTATATTTGGGTTTTGGTTTCTGGTCTGTTTTATCATCTTTATAATCAATCATCTTATCAAGCATTGGATGAAATTAGTCCATTAACTTTCTCTGTTGGAAATACAATGAAGAGAGTGGTAGTGATTGTGTCTTCGATTTTGGTGTTCCGGAATCCGGTTAGGCCTCTTAATGGTCTTGGATCTGCCATTGCTATTCTTGGGACTTTTCTGTATTCACAAGCTACAGCTGCAAAGAAAGCAAAGAAAATTGAAGGGGAAAAGAGTAGTTAG

Protein sequence

>MS.gene74587.t1

MLVLWSLKLQPCPKISKPFIFALLGPALFHTIGHISACVSFSKVAVSFTHVIKSAEPVFSVIFSSVLGDRYPIQVWLSILPIVLGCSLAAVTEVSFNVGGLWCALISNVGFVLRNIYSKKSLQNFKEVDGLNLYGWITILSFMYLFPVAIFVEGSQWIPGYYKALEAIGTPSTFYIWVLVSGLFYHLYNQSSYQALDEISPLTFSVGNTMKRVVVIVSSILVFRNPVRPLNGLGSAIAILGTFLYSQATAAKKAKKIEGEKSS