AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048769

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048769.t1 MTR_8g096990 92.449 437 28 1 1 437 1 432 0.0 825
MS.gene048769.t1 MTR_5g080890 76.538 439 96 4 1 437 1 434 0.0 679
MS.gene048769.t1 MTR_5g080960 76.765 439 95 4 1 437 1 434 0.0 679
MS.gene048769.t1 MTR_8g038250 75.847 443 74 3 1 437 1 416 0.0 678
MS.gene048769.t1 MTR_8g038280 72.540 437 119 1 1 437 1 436 0.0 659
MS.gene048769.t1 MTR_5g044770 72.540 437 114 2 1 437 1 431 0.0 648
MS.gene048769.t1 MTR_6g090290 67.735 437 139 2 1 436 1 436 0.0 585
MS.gene048769.t1 MTR_8g038310 63.387 437 135 4 1 436 1 413 0.0 551
MS.gene048769.t1 MTR_4g109760 67.277 437 105 5 1 437 1 399 0.0 539
MS.gene048769.t1 MTR_3g028120 53.444 421 183 7 25 437 24 439 5.22e-151 436
MS.gene048769.t1 MTR_6g088760 55.231 411 168 8 1 402 1 404 1.69e-146 427
MS.gene048769.t1 MTR_2g067830 53.398 412 179 8 32 436 1 406 3.02e-146 422
MS.gene048769.t1 MTR_5g038360 50.114 437 201 9 9 436 8 436 1.02e-138 404
MS.gene048769.t1 MTR_2g105300 51.834 409 162 9 32 433 1 381 8.91e-134 390
MS.gene048769.t1 MTR_0024s0040 51.196 418 185 8 30 436 1 410 4.58e-130 382
MS.gene048769.t1 MTR_5g044570 47.651 447 187 12 1 436 1 411 4.82e-127 374
MS.gene048769.t1 MTR_0036s0070 43.722 446 221 11 1 436 1 426 4.85e-118 351
MS.gene048769.t1 MTR_0036s0050 43.237 451 225 11 1 436 1 435 1.07e-113 340
MS.gene048769.t1 MTR_0036s0110 41.138 457 233 11 1 436 1 442 2.16e-107 325
MS.gene048769.t1 MTR_5g008070 41.469 422 221 10 21 435 23 425 1.68e-99 304
MS.gene048769.t1 MTR_2g059490 41.190 437 214 11 9 437 10 411 2.70e-93 288
MS.gene048769.t1 MTR_5g008150 39.953 423 209 11 22 436 27 412 1.18e-91 283
MS.gene048769.t1 MTR_2g059460 40.426 423 202 12 23 436 25 406 1.02e-88 276
MS.gene048769.t1 MTR_5g008110 37.529 437 224 12 7 436 21 415 1.00e-87 273
MS.gene048769.t1 MTR_3g097150 37.116 423 239 10 27 437 42 449 1.83e-84 266
MS.gene048769.t1 MTR_1g060750 37.592 407 212 11 40 436 2 376 1.21e-78 249
MS.gene048769.t1 MTR_5g008090 38.487 304 168 9 142 436 21 314 2.20e-59 197
MS.gene048769.t1 MTR_4g045857 40.323 310 144 10 14 316 13 288 4.23e-58 192
MS.gene048769.t1 MTR_7g117430 32.861 423 253 14 28 434 77 484 2.16e-56 194
MS.gene048769.t1 MTR_1g115210 32.176 432 257 14 21 434 73 486 2.19e-55 191
MS.gene048769.t1 MTR_1g039270 32.723 437 239 18 28 434 58 469 1.24e-53 186
MS.gene048769.t1 MTR_2g028190 36.813 364 205 13 84 434 100 451 6.86e-52 181
MS.gene048769.t1 MTR_6g088765 43.802 242 97 5 162 402 5 208 1.54e-50 171
MS.gene048769.t1 MTR_3g046300 29.437 462 275 15 11 437 16 461 1.92e-46 166
MS.gene048769.t1 MTR_1g111350 32.778 360 212 12 84 434 129 467 2.05e-46 167
MS.gene048769.t1 MTR_6g085010 33.803 355 210 11 86 434 156 491 3.39e-45 164
MS.gene048769.t1 MTR_4g007740 33.609 363 210 12 87 435 133 478 1.55e-43 159
MS.gene048769.t1 MTR_5g091130 30.993 413 255 13 47 437 152 556 1.60e-41 155
MS.gene048769.t1 MTR_2g070430 27.813 471 288 16 2 436 3 457 7.84e-39 145
MS.gene048769.t1 MTR_3g053710 31.250 368 218 12 85 434 116 466 3.10e-38 144
MS.gene048769.t1 MTR_2g055840 29.101 378 238 10 78 434 5 373 3.93e-38 142
MS.gene048769.t1 MTR_8g103880 31.105 344 212 13 87 422 117 443 8.34e-38 143
MS.gene048769.t1 MTR_3g068000 27.649 387 218 16 89 434 76 441 7.94e-31 124
MS.gene048769.t1 MTR_7g080230 28.082 438 266 16 30 435 11 431 2.83e-30 122
MS.gene048769.t1 MTR_4g008110 55.085 118 25 1 161 278 48 137 1.04e-29 112
MS.gene048769.t1 MTR_1g078240 28.982 383 220 16 85 435 68 430 6.18e-29 118
MS.gene048769.t1 MTR_3g065160 25.201 373 237 13 88 437 76 429 7.07e-28 115
MS.gene048769.t1 MTR_1g078240 29.167 384 219 17 85 435 68 431 1.02e-27 115
MS.gene048769.t1 MTR_7g105850 28.131 487 265 23 9 436 7 467 1.28e-27 114
MS.gene048769.t1 MTR_8g094590 27.764 407 230 17 64 437 52 427 2.93e-27 113
MS.gene048769.t1 MTR_4g095270 25.542 415 266 15 41 437 37 426 5.12e-27 114
MS.gene048769.t1 MTR_4g095270 25.542 415 266 15 41 437 37 426 5.71e-27 114
MS.gene048769.t1 MTR_3g053980 29.589 365 224 11 84 434 140 485 6.23e-27 113
MS.gene048769.t1 MTR_2g084550 31.675 382 211 19 85 435 76 438 6.93e-27 112
MS.gene048769.t1 MTR_5g016260 25.798 376 230 15 84 437 86 434 1.73e-26 112
MS.gene048769.t1 MTR_5g016260 25.798 376 230 15 84 437 86 434 2.46e-26 112
MS.gene048769.t1 MTR_6g084710 26.402 428 245 18 49 434 151 550 2.83e-26 111
MS.gene048769.t1 MTR_3g096930 24.938 401 249 13 64 437 62 437 1.27e-25 108
MS.gene048769.t1 MTR_4g056420 29.396 381 220 18 85 435 76 437 2.10e-25 108
MS.gene048769.t1 MTR_7g062360 27.865 384 225 18 89 437 62 428 4.66e-25 107
MS.gene048769.t1 MTR_1g061940 29.122 467 264 26 8 435 7 445 6.38e-25 107
MS.gene048769.t1 MTR_5g077850 25.707 389 219 16 88 437 83 440 6.76e-25 106
MS.gene048769.t1 MTR_8g094590 28.941 387 212 17 64 422 52 403 8.55e-25 105
MS.gene048769.t1 MTR_7g021510 26.904 394 222 17 82 435 67 434 9.17e-25 106
MS.gene048769.t1 MTR_7g102750 28.460 383 221 19 85 435 80 441 1.37e-24 106
MS.gene048769.t1 MTR_2g063110 24.738 477 276 19 28 434 45 508 3.31e-24 105
MS.gene048769.t1 MTR_0945s0010 40.476 126 71 3 315 437 1 125 3.89e-24 98.6
MS.gene048769.t1 MTR_1g078240 30.599 317 176 14 85 377 68 364 6.16e-24 103
MS.gene048769.t1 MTR_4g100990 28.365 416 218 21 86 437 74 473 8.91e-24 103
MS.gene048769.t1 MTR_8g094590 31.707 287 155 11 64 338 52 309 1.02e-23 101
MS.gene048769.t1 MTR_1g029810 25.849 383 228 18 85 437 56 412 1.90e-23 102
MS.gene048769.t1 MTR_6g084710 27.647 340 199 14 49 369 151 462 2.21e-23 102
MS.gene048769.t1 MTR_1g029810 25.849 383 228 18 85 437 56 412 2.42e-23 102
MS.gene048769.t1 MTR_4g023650 26.173 405 242 18 66 437 42 422 5.09e-23 100
MS.gene048769.t1 MTR_1g037630 26.761 426 275 15 25 435 26 429 2.17e-22 99.4
MS.gene048769.t1 MTR_2g103340 42.241 116 23 1 21 136 34 105 3.28e-22 94.0
MS.gene048769.t1 MTR_2g103340 48.052 77 35 2 355 431 88 159 4.06e-12 65.1
MS.gene048769.t1 MTR_8g039540 26.327 452 288 17 6 435 6 434 2.47e-21 96.3
MS.gene048769.t1 MTR_4g023630 24.631 406 247 16 66 437 42 422 5.52e-21 95.1
MS.gene048769.t1 MTR_5g022880 27.110 391 225 16 79 434 96 461 9.69e-21 95.1
MS.gene048769.t1 MTR_7g102750 29.755 326 181 17 85 383 80 384 2.33e-20 93.2
MS.gene048769.t1 MTR_3g014290 28.134 359 226 13 87 435 81 417 3.52e-20 92.4
MS.gene048769.t1 MTR_7g080230 28.125 352 209 15 110 435 28 361 1.21e-19 90.9
MS.gene048769.t1 MTR_5g012490 26.025 488 278 25 9 435 5 470 1.28e-19 91.3
MS.gene048769.t1 MTR_8g075090 25.984 381 219 18 87 436 113 461 3.59e-18 87.0
MS.gene048769.t1 MTR_8g075090 26.260 377 215 18 92 437 118 462 7.06e-18 85.9
MS.gene048769.t1 MTR_5g077920 27.078 373 234 14 88 436 70 428 9.89e-18 85.5
MS.gene048769.t1 MTR_5g061690 28.011 357 199 20 93 422 108 433 2.25e-17 84.7
MS.gene048769.t1 MTR_3g087780 24.027 437 261 18 28 422 27 434 1.23e-16 82.4
MS.gene048769.t1 MTR_3g006820 23.797 395 241 18 78 437 46 415 3.69e-16 80.5
MS.gene048769.t1 MTR_8g075010 25.397 378 223 18 90 437 104 452 5.79e-16 80.5
MS.gene048769.t1 MTR_8g075090 26.590 346 199 16 92 410 118 435 1.61e-15 79.0
MS.gene048769.t1 MTR_3g100500 27.208 419 255 20 40 434 45 437 3.45e-15 77.8
MS.gene048769.t1 MTR_5g080330 25.067 375 184 18 103 434 2 322 9.31e-14 73.2
MS.gene048769.t1 MTR_5g061690 27.152 302 173 17 138 422 23 294 6.33e-13 70.5
MS.gene048769.t1 MTR_1g072380 22.556 399 222 17 86 429 56 422 5.56e-11 64.7
MS.gene048769.t1 MTR_2g037710 25.455 220 131 8 224 435 12 206 6.11e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048769.t1 AT1G64830 51.208 414 194 4 26 437 24 431 1.37e-139 407
MS.gene048769.t1 AT5G33340 51.208 414 193 5 27 437 30 437 2.52e-134 393
MS.gene048769.t1 AT2G35615 44.921 443 218 8 14 435 7 444 1.02e-114 343
MS.gene048769.t1 AT1G31450 43.318 434 224 8 16 435 17 442 4.87e-105 319
MS.gene048769.t1 AT2G28010 39.819 442 208 11 3 437 4 394 6.27e-88 273
MS.gene048769.t1 AT2G28040 40.281 427 199 12 16 437 18 393 2.66e-83 261
MS.gene048769.t1 AT2G28040 41.007 417 190 12 26 437 19 384 6.17e-83 260
MS.gene048769.t1 AT2G28030 41.463 369 185 8 74 437 48 390 1.24e-77 246
MS.gene048769.t1 AT2G28220 37.303 445 221 11 3 437 4 400 3.81e-77 246
MS.gene048769.t1 AT2G03200 36.156 437 238 12 25 437 42 461 1.55e-75 243
MS.gene048769.t1 AT2G28225 40.751 373 186 9 74 437 54 400 1.06e-74 239
MS.gene048769.t1 AT4G30030 37.867 375 195 15 79 437 70 422 1.12e-56 193
MS.gene048769.t1 AT1G01300 35.792 366 202 13 84 435 139 485 1.45e-55 191
MS.gene048769.t1 AT3G61820 34.848 396 222 16 55 434 108 483 1.55e-52 183
MS.gene048769.t1 AT3G18490 31.409 433 261 16 16 434 90 500 4.63e-50 177
MS.gene048769.t1 AT3G20015 29.186 442 264 15 20 434 51 470 2.26e-47 169
MS.gene048769.t1 AT5G10760 34.419 430 241 17 23 434 58 464 4.32e-47 168
MS.gene048769.t1 AT1G25510 33.609 363 205 14 84 434 145 483 1.04e-46 168
MS.gene048769.t1 AT2G23945 28.294 463 277 15 9 437 10 451 1.32e-43 159
MS.gene048769.t1 AT5G36260 32.723 382 203 15 85 435 76 434 1.35e-43 159
MS.gene048769.t1 AT3G25700 29.861 432 248 17 39 435 39 450 1.41e-43 159
MS.gene048769.t1 AT5G10770 34.341 364 209 13 84 435 129 474 3.19e-42 155
MS.gene048769.t1 AT1G79720 32.454 379 194 16 87 434 135 482 1.47e-40 151
MS.gene048769.t1 AT3G59080 31.969 391 231 16 72 437 155 535 9.11e-40 150
MS.gene048769.t1 AT4G30040 30.958 449 251 21 9 429 10 427 1.72e-39 147
MS.gene048769.t1 AT2G42980 31.807 393 231 17 72 437 145 527 3.76e-39 148
MS.gene048769.t1 AT1G65240 30.628 382 208 16 85 435 72 427 2.57e-37 142
MS.gene048769.t1 AT3G02740 30.649 385 223 15 74 435 77 440 7.73e-37 141
MS.gene048769.t1 AT3G02740 30.376 372 220 14 87 435 10 365 2.68e-36 138
MS.gene048769.t1 AT3G12700 25.778 450 301 15 5 435 26 461 1.27e-34 134
MS.gene048769.t1 AT2G39710 29.921 381 205 19 89 429 67 425 4.64e-34 132
MS.gene048769.t1 AT3G59080 30.052 386 209 15 72 437 155 499 2.84e-30 122
MS.gene048769.t1 AT4G33490 24.878 410 238 14 68 437 35 414 2.59e-29 119
MS.gene048769.t1 AT1G66180 27.586 377 226 17 88 437 73 429 7.35e-29 117
MS.gene048769.t1 AT5G43100 26.829 369 234 15 84 437 73 420 5.94e-28 115
MS.gene048769.t1 AT5G43100 26.829 369 234 15 84 437 73 420 1.07e-27 116
MS.gene048769.t1 AT4G12920 28.760 379 231 15 67 434 37 387 1.35e-27 113
MS.gene048769.t1 AT5G37540 27.614 373 229 14 88 434 81 438 2.29e-27 114
MS.gene048769.t1 AT5G22850 29.973 377 224 16 85 435 79 441 4.09e-27 113
MS.gene048769.t1 AT5G02190 26.316 380 220 16 96 434 82 442 5.05e-27 112
MS.gene048769.t1 AT3G50050 26.049 453 282 19 12 437 13 439 6.40e-27 114
MS.gene048769.t1 AT1G09750 28.649 370 221 16 85 434 102 448 9.79e-27 112
MS.gene048769.t1 AT1G44130 25.743 404 241 14 68 437 24 402 2.22e-26 110
MS.gene048769.t1 AT3G54400 27.110 391 247 13 62 435 56 425 2.64e-26 110
MS.gene048769.t1 AT1G77480 25.526 380 234 15 85 437 65 422 3.77e-25 107
MS.gene048769.t1 AT1G77480 25.526 380 234 15 85 437 65 422 6.53e-25 107
MS.gene048769.t1 AT4G33490 27.492 331 187 12 68 375 32 332 1.72e-24 105
MS.gene048769.t1 AT5G45120 26.650 409 226 16 87 434 83 478 2.42e-24 105
MS.gene048769.t1 AT1G05840 27.632 380 228 15 85 435 78 439 7.96e-24 103
MS.gene048769.t1 AT3G52500 26.464 461 258 21 40 435 24 468 1.82e-22 99.8
MS.gene048769.t1 AT2G36670 28.117 377 229 16 85 435 98 458 8.39e-22 98.2
MS.gene048769.t1 AT1G49050 23.925 372 221 15 104 436 49 397 9.24e-22 97.1
MS.gene048769.t1 AT1G49050 24.372 398 236 18 80 436 197 570 9.84e-22 98.2
MS.gene048769.t1 AT1G08210 28.647 377 226 16 85 435 116 475 1.10e-21 97.8
MS.gene048769.t1 AT1G08210 28.647 377 226 16 85 435 130 489 1.10e-21 97.8
MS.gene048769.t1 AT1G08210 28.647 377 226 16 85 435 82 441 1.17e-21 97.4
MS.gene048769.t1 AT2G36670 28.000 375 228 16 87 435 105 463 2.78e-21 96.7
MS.gene048769.t1 AT2G36670 28.000 375 228 16 87 435 34 392 3.16e-21 95.9
MS.gene048769.t1 AT2G36670 28.000 375 228 16 87 435 34 392 3.16e-21 95.9
MS.gene048769.t1 AT2G17760 27.297 370 213 17 92 434 109 449 5.32e-21 95.9
MS.gene048769.t1 AT5G07030 26.293 464 289 18 2 435 15 455 4.77e-20 92.4
MS.gene048769.t1 AT1G08210 26.733 404 226 16 85 435 82 468 2.15e-17 84.7
MS.gene048769.t1 AT1G49050 26.316 285 170 14 80 347 197 458 2.86e-17 84.3
MS.gene048769.t1 AT3G42550 24.251 367 206 12 87 435 78 390 2.28e-16 81.3
MS.gene048769.t1 AT3G51350 25.469 373 231 16 87 434 102 452 4.06e-16 80.9
MS.gene048769.t1 AT3G25700 29.592 196 111 7 39 221 39 220 5.80e-16 79.3
MS.gene048769.t1 AT4G35880 24.538 379 221 18 90 437 110 454 1.47e-13 72.8
MS.gene048769.t1 AT4G16563 26.341 410 211 21 104 437 100 494 4.89e-13 71.2
MS.gene048769.t1 AT3G51330 24.932 369 229 16 92 434 107 453 1.22e-11 66.6
MS.gene048769.t1 AT3G51330 24.932 369 229 16 92 434 107 453 1.29e-11 67.0
MS.gene048769.t1 AT4G35880 24.425 348 206 16 90 410 110 427 5.92e-11 64.7

Find 72 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTAATATTTATAATAATT+TGG 0.118502 4.3:-6448403 MS.gene048769:CDS
GGAGATGCCGCTGTGGTTTC+TGG 0.147563 4.3:-6448597 MS.gene048769:CDS
TGTGAATATACTATTGCTTA+TGG 0.243798 4.3:-6448903 MS.gene048769:CDS
GACAATACAATGTCGTTTAA+AGG 0.262527 4.3:-6448774 MS.gene048769:CDS
TTAAAATGCGCTGTTATTAG+AGG 0.262980 4.3:+6448282 None:intergenic
ACAATGCCATATATCTTAAA+TGG 0.279082 4.3:+6449095 None:intergenic
GGAAACAAAAGAATAGAATT+TGG 0.288475 4.3:-6448495 MS.gene048769:CDS
TCATTTCCTAAAACTGTGAT+AGG 0.290097 4.3:-6448807 MS.gene048769:CDS
TCAGGAATTACAGTTGATTC+AGG 0.295216 4.3:+6449158 None:intergenic
AGCCCACCATTTAAGATATA+TGG 0.321843 4.3:-6449101 MS.gene048769:CDS
AAGACAAGCAAACTCAGTTT+TGG 0.327541 4.3:-6448618 MS.gene048769:CDS
TGTTAAATAGTAGAAAACTA+TGG 0.332231 4.3:+6448536 None:intergenic
GGTAACATCATAATTGATTC+AGG 0.364855 4.3:-6448447 MS.gene048769:CDS
AAATTATTATAAATATTAGA+TGG 0.365192 4.3:+6448405 None:intergenic
ATGGAAACAAAGGTACTAAT+AGG 0.366817 4.3:+6448237 None:intergenic
ATGAGATACTCACCATTATC+AGG 0.381520 4.3:+6449140 None:intergenic
CTTAGCTCAGCAGAATTTGT+TGG 0.389893 4.3:-6448154 MS.gene048769:CDS
ATACAGGTAGTGACATTGTT+TGG 0.394829 4.3:-6449070 MS.gene048769:CDS
TCAACTGTAATTCCTGATAA+TGG 0.402401 4.3:-6449152 MS.gene048769:CDS
TCTTCGGGCGTTGCTATCTT+CGG 0.415446 4.3:-6448180 MS.gene048769:CDS
CAAAGGTTTAATTGTTGAGT+TGG 0.422727 4.3:+6448336 None:intergenic
TCTGGTATAGTTGGCCTTGG+AGG 0.436740 4.3:-6448744 MS.gene048769:CDS
TGGTGGGCTGCCAATTGAAT+AGG 0.439433 4.3:+6449115 None:intergenic
ACACAACTTGGATCGTCAAT+TGG 0.440174 4.3:-6448702 MS.gene048769:CDS
AGCTCTGGTATAGTTGGCCT+TGG 0.443123 4.3:-6448747 MS.gene048769:CDS
GTTAAATAGTAGAAAACTAT+GGG 0.448636 4.3:+6448537 None:intergenic
GTTTAAACTTAGGAGTGGTT+TGG 0.455654 4.3:+6449015 None:intergenic
TCGTTTAAAGGCGCAAGCTC+TGG 0.458230 4.3:-6448762 MS.gene048769:CDS
TTTAAACGACATTGTATTGT+CGG 0.466344 4.3:+6448776 None:intergenic
TATCTCATGACCTATTCAAT+TGG 0.471723 4.3:-6449125 MS.gene048769:CDS
CCTAAAACTGTGATAGGATG+TGG 0.472735 4.3:-6448801 MS.gene048769:CDS
GGTATAGTTGGCCTTGGAGG+TGG 0.473391 4.3:-6448741 MS.gene048769:CDS
AAAAGAAGCAATGAAACAAA+GGG 0.475787 4.3:+6449346 None:intergenic
GTGCGGCATTGACAACTTGT+TGG 0.485070 4.3:+6449234 None:intergenic
GGCGCAAGCTCTGGTATAGT+TGG 0.493766 4.3:-6448753 MS.gene048769:CDS
GGAATCAGCCGTGGTAGAAT+CGG 0.498390 4.3:-6448382 MS.gene048769:CDS
ATATATGGCATTGTTGATAC+AGG 0.504947 4.3:-6449086 MS.gene048769:CDS
ACCTTTGTTTCCATTGCCAA+TGG 0.512736 4.3:-6448228 MS.gene048769:CDS
GATAGCAACGCCCGAAGAAG+CGG 0.523072 4.3:+6448185 None:intergenic
GCAAACAATGCCATTGGCAA+TGG 0.526861 4.3:+6448218 None:intergenic
ATGCCATATATCTTAAATGG+TGG 0.536932 4.3:+6449098 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.540009 4.3:-6448516 MS.gene048769:CDS
ATCATCAAATGGTGGTGATG+AGG 0.548330 4.3:-6448469 MS.gene048769:CDS
AGCAAAGCAAACAATGCCAT+TGG 0.555164 4.3:+6448212 None:intergenic
GCTCAGCAGAATTTGTTGGT+TGG 0.563160 4.3:-6448150 MS.gene048769:CDS
CTTACATTAGAGTCCTCTAC+TGG 0.564370 4.3:-6448840 MS.gene048769:CDS
TGAAATATGACCGCCAGTAG+AGG 0.573661 4.3:+6448827 None:intergenic
TGCCATATATCTTAAATGGT+GGG 0.574669 4.3:+6449099 None:intergenic
GAATTTGGCAGATCATCAAA+TGG 0.575497 4.3:-6448480 MS.gene048769:CDS
TCATCAAATGGTGGTGATGA+GGG 0.576269 4.3:-6448468 MS.gene048769:CDS
TACACTTTGCTACAATCAGT+AGG 0.577745 4.3:+6448093 None:intergenic
TGCTATAGTACCACATCAGA+CGG 0.579957 4.3:-6448315 MS.gene048769:CDS
TGACATAGGTTAGATGAACA+TGG 0.583072 4.3:+6448963 None:intergenic
TATCTCTCACAGATTGACAT+AGG 0.588294 4.3:+6448949 None:intergenic
GCCATTGGCAATGGAAACAA+AGG 0.590979 4.3:+6448227 None:intergenic
TGATGGTTTAAACTTAGGAG+TGG 0.593505 4.3:+6449010 None:intergenic
GTGTCTCTTATAACACAACT+TGG 0.612159 4.3:-6448714 MS.gene048769:CDS
AAAATTATATCCGTCTGATG+TGG 0.617721 4.3:+6448305 None:intergenic
CTGATGTGGTACTATAGCAA+AGG 0.621790 4.3:+6448319 None:intergenic
GAGACACGGGACCACCTCCA+AGG 0.631998 4.3:+6448730 None:intergenic
GAGTTTGACTCAAAAGACAA+TGG 0.632515 4.3:+6448654 None:intergenic
GACATAGGTTAGATGAACAT+GGG 0.639950 4.3:+6448964 None:intergenic
TAATAATTTGGAATCAGCCG+TGG 0.648028 4.3:-6448391 MS.gene048769:CDS
AAGTTGTGTTATAAGAGACA+CGG 0.651825 4.3:+6448716 None:intergenic
GGAGATCATTCACATTCACA+AGG 0.667677 4.3:-6448882 MS.gene048769:CDS
AGTTGTGTTATAAGAGACAC+GGG 0.672510 4.3:+6448717 None:intergenic
ACATTAGAGTCCTCTACTGG+CGG 0.682517 4.3:-6448837 MS.gene048769:CDS
TTTGGCAGATCATCAAATGG+TGG 0.685338 4.3:-6448477 MS.gene048769:CDS
TTAAATAGTAGAAAACTATG+GGG 0.716714 4.3:+6448538 None:intergenic
ACGGTTGATAGAACGACGTG+CGG 0.717425 4.3:+6449217 None:intergenic
GAGTTTCACCGATTCTACCA+CGG 0.727068 4.3:+6448374 None:intergenic
ATCATCACCAGAAACCACAG+CGG 0.787801 4.3:+6448590 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATATTCACAAAAATTTT+GGG + chr4.3:6448564-6448583 None:intergenic 15.0%
!!! TAGTATATTCACAAAAATTT+TGG + chr4.3:6448565-6448584 None:intergenic 15.0%
!! GTTAAATAGTAGAAAACTAT+GGG + chr4.3:6448941-6448960 None:intergenic 20.0%
!! TGTTAAATAGTAGAAAACTA+TGG + chr4.3:6448942-6448961 None:intergenic 20.0%
!! TTAAATAGTAGAAAACTATG+GGG + chr4.3:6448940-6448959 None:intergenic 20.0%
!!! AGTTTTCTACTATTTAACAT+TGG - chr4.3:6448943-6448962 MS.gene048769:CDS 20.0%
!!! TTTTGTAGAAATGATTAACA+CGG + chr4.3:6448280-6448299 None:intergenic 20.0%
! AAAAAGAAGCAATGAAACAA+AGG + chr4.3:6448133-6448152 None:intergenic 25.0%
! AAAAGAAGCAATGAAACAAA+GGG + chr4.3:6448132-6448151 None:intergenic 25.0%
! AAAGAAAAAAAGGGTTAGAA+AGG + chr4.3:6448108-6448127 None:intergenic 25.0%
! AACAAAGGGAAAAGAAAAAA+AGG + chr4.3:6448118-6448137 None:intergenic 25.0%
! ACAAAGGGAAAAGAAAAAAA+GGG + chr4.3:6448117-6448136 None:intergenic 25.0%
! ACAATGCCATATATCTTAAA+TGG + chr4.3:6448383-6448402 None:intergenic 25.0%
! GGAAACAAAAGAATAGAATT+TGG - chr4.3:6448980-6448999 MS.gene048769:CDS 25.0%
! TGTGAATATACTATTGCTTA+TGG - chr4.3:6448572-6448591 MS.gene048769:CDS 25.0%
! TTTAAACGACATTGTATTGT+CGG + chr4.3:6448702-6448721 None:intergenic 25.0%
!!! ATGACACAGTATTTTGTTTA+AGG + chr4.3:6449357-6449376 None:intergenic 25.0%
!!! GATTTTGATGGTTTAAACTT+AGG + chr4.3:6448473-6448492 None:intergenic 25.0%
!!! TTGTAAGTTGATGATTTTGA+TGG + chr4.3:6448485-6448504 None:intergenic 25.0%
AAAATTATATCCGTCTGATG+TGG + chr4.3:6449173-6449192 None:intergenic 30.0%
AAGTTGTGTTATAAGAGACA+CGG + chr4.3:6448762-6448781 None:intergenic 30.0%
ATATATGGCATTGTTGATAC+AGG - chr4.3:6448389-6448408 MS.gene048769:CDS 30.0%
ATGCCATATATCTTAAATGG+TGG + chr4.3:6448380-6448399 None:intergenic 30.0%
CAAAGGTTTAATTGTTGAGT+TGG + chr4.3:6449142-6449161 None:intergenic 30.0%
TATCTCATGACCTATTCAAT+TGG - chr4.3:6448350-6448369 MS.gene048769:CDS 30.0%
TCAACTGTAATTCCTGATAA+TGG - chr4.3:6448323-6448342 MS.gene048769:CDS 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr4.3:6448668-6448687 MS.gene048769:CDS 30.0%
TGCCATATATCTTAAATGGT+GGG + chr4.3:6448379-6448398 None:intergenic 30.0%
TTAAAATGCGCTGTTATTAG+AGG + chr4.3:6449196-6449215 None:intergenic 30.0%
! CTAATAACAGCGCATTTTAA+AGG - chr4.3:6449196-6449215 MS.gene048769:CDS 30.0%
! GACAATACAATGTCGTTTAA+AGG - chr4.3:6448701-6448720 MS.gene048769:CDS 30.0%
! GGTAACATCATAATTGATTC+AGG - chr4.3:6449028-6449047 MS.gene048769:CDS 30.0%
! TGGTTGTAACATTTTTCACA+AGG + chr4.3:6448443-6448462 None:intergenic 30.0%
! TTTTCTCATGCGCTAAATAA+TGG - chr4.3:6448149-6448168 MS.gene048769:CDS 30.0%
!! ATGGAAACAAAGGTACTAAT+AGG + chr4.3:6449241-6449260 None:intergenic 30.0%
AGTTGTGTTATAAGAGACAC+GGG + chr4.3:6448761-6448780 None:intergenic 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr4.3:6448405-6448424 MS.gene048769:CDS 35.0%
ATGAGATACTCACCATTATC+AGG + chr4.3:6448338-6448357 None:intergenic 35.0%
GAATTTGGCAGATCATCAAA+TGG - chr4.3:6448995-6449014 MS.gene048769:CDS 35.0%
GACATAGGTTAGATGAACAT+GGG + chr4.3:6448514-6448533 None:intergenic 35.0%
GAGTTTGACTCAAAAGACAA+TGG + chr4.3:6448824-6448843 None:intergenic 35.0%
GTGTCTCTTATAACACAACT+TGG - chr4.3:6448761-6448780 MS.gene048769:CDS 35.0%
GTTTAAACTTAGGAGTGGTT+TGG + chr4.3:6448463-6448482 None:intergenic 35.0%
TACACTTTGCTACAATCAGT+AGG + chr4.3:6449385-6449404 None:intergenic 35.0%
TATCTCTCACAGATTGACAT+AGG + chr4.3:6448529-6448548 None:intergenic 35.0%
TGACATAGGTTAGATGAACA+TGG + chr4.3:6448515-6448534 None:intergenic 35.0%
TGATGGTTTAAACTTAGGAG+TGG + chr4.3:6448468-6448487 None:intergenic 35.0%
! AAGACAAGCAAACTCAGTTT+TGG - chr4.3:6448857-6448876 MS.gene048769:CDS 35.0%
! AGCCCACCATTTAAGATATA+TGG - chr4.3:6448374-6448393 MS.gene048769:CDS 35.0%
! TAATAATTTGGAATCAGCCG+TGG - chr4.3:6449084-6449103 MS.gene048769:CDS 35.0%
! TCAGGAATTACAGTTGATTC+AGG + chr4.3:6448320-6448339 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr4.3:6448959-6448978 MS.gene048769:CDS 35.0%
!! ATGGTGATTTTGAAGAGTCA+CGG + chr4.3:6448196-6448215 None:intergenic 35.0%
!!! ATGGGGTGTTTTTTCACTAT+AGG + chr4.3:6448923-6448942 None:intergenic 35.0%
!!! TGTTGGTACTTGTTTTGTGT+AGG + chr4.3:6448227-6448246 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTAGAA+TGG + chr4.3:6448215-6448234 None:intergenic 35.0%
ACACAACTTGGATCGTCAAT+TGG - chr4.3:6448773-6448792 MS.gene048769:CDS 40.0%
AGCAAAGCAAACAATGCCAT+TGG + chr4.3:6449266-6449285 None:intergenic 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr4.3:6448674-6448693 MS.gene048769:CDS 40.0%
CTGATGTGGTACTATAGCAA+AGG + chr4.3:6449159-6449178 None:intergenic 40.0%
CTTACATTAGAGTCCTCTAC+TGG - chr4.3:6448635-6448654 MS.gene048769:CDS 40.0%
CTTAGCTCAGCAGAATTTGT+TGG - chr4.3:6449321-6449340 MS.gene048769:CDS 40.0%
GGAGATCATTCACATTCACA+AGG - chr4.3:6448593-6448612 MS.gene048769:CDS 40.0%
TGCTATAGTACCACATCAGA+CGG - chr4.3:6449160-6449179 MS.gene048769:CDS 40.0%
TTTGGCAGATCATCAAATGG+TGG - chr4.3:6448998-6449017 MS.gene048769:CDS 40.0%
! ACCTTTGTTTCCATTGCCAA+TGG - chr4.3:6449247-6449266 MS.gene048769:CDS 40.0%
! ATCATCAAATGGTGGTGATG+AGG - chr4.3:6449006-6449025 MS.gene048769:CDS 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr4.3:6448677-6448696 None:intergenic 40.0%
! TCATCAAATGGTGGTGATGA+GGG - chr4.3:6449007-6449026 MS.gene048769:CDS 40.0%
!! AACAGCGCATTTTAAAGGTG+CGG - chr4.3:6449201-6449220 MS.gene048769:CDS 40.0%
!! CTTTGCTTTTACCGCTTCTT+CGG - chr4.3:6449279-6449298 MS.gene048769:CDS 40.0%
!! TTTGCTTTTACCGCTTCTTC+GGG - chr4.3:6449280-6449299 MS.gene048769:CDS 40.0%
!!! GGTACTTGTTTTGTGTAGGT+TGG + chr4.3:6448223-6448242 None:intergenic 40.0%
ACATTAGAGTCCTCTACTGG+CGG - chr4.3:6448638-6448657 MS.gene048769:CDS 45.0%
ATCATCACCAGAAACCACAG+CGG + chr4.3:6448888-6448907 None:intergenic 45.0%
GCAAACAATGCCATTGGCAA+TGG + chr4.3:6449260-6449279 None:intergenic 45.0%
GCCATTGGCAATGGAAACAA+AGG + chr4.3:6449251-6449270 None:intergenic 45.0%
TGAAATATGACCGCCAGTAG+AGG + chr4.3:6448651-6448670 None:intergenic 45.0%
!! GAGTTTCACCGATTCTACCA+CGG + chr4.3:6449104-6449123 None:intergenic 45.0%
!! GCTCAGCAGAATTTGTTGGT+TGG - chr4.3:6449325-6449344 MS.gene048769:CDS 45.0%
!! GTGATTTTGAAGAGTCACGG+TGG + chr4.3:6448193-6448212 None:intergenic 45.0%
!! AAATTATTATAAATATTAGA+TGG + chr4.3:6449073-6449092 None:intergenic 5.0%
!! ATCTAATATTTATAATAATT+TGG - chr4.3:6449072-6449091 MS.gene048769:CDS 5.0%
GGAATCAGCCGTGGTAGAAT+CGG - chr4.3:6449093-6449112 MS.gene048769:CDS 50.0%
! AGCTCTGGTATAGTTGGCCT+TGG - chr4.3:6448728-6448747 MS.gene048769:CDS 50.0%
! TCGTTTAAAGGCGCAAGCTC+TGG - chr4.3:6448713-6448732 MS.gene048769:CDS 50.0%
! TCTTCGGGCGTTGCTATCTT+CGG - chr4.3:6449295-6449314 MS.gene048769:CDS 50.0%
!! ACGGTTGATAGAACGACGTG+CGG + chr4.3:6448261-6448280 None:intergenic 50.0%
!! GTGCGGCATTGACAACTTGT+TGG + chr4.3:6448244-6448263 None:intergenic 50.0%
!! TCTGGTATAGTTGGCCTTGG+AGG - chr4.3:6448731-6448750 MS.gene048769:CDS 50.0%
!! TGGTGGGCTGCCAATTGAAT+AGG + chr4.3:6448363-6448382 None:intergenic 50.0%
GATAGCAACGCCCGAAGAAG+CGG + chr4.3:6449293-6449312 None:intergenic 55.0%
! GGCGCAAGCTCTGGTATAGT+TGG - chr4.3:6448722-6448741 MS.gene048769:CDS 55.0%
!! GGTATAGTTGGCCTTGGAGG+TGG - chr4.3:6448734-6448753 MS.gene048769:CDS 55.0%
!!! CAGTTTTGGAGATGCCGCTG+TGG - chr4.3:6448871-6448890 MS.gene048769:CDS 55.0%
GGAGATGCCGCTGTGGTTTC+TGG - chr4.3:6448878-6448897 MS.gene048769:CDS 60.0%
GAGACACGGGACCACCTCCA+AGG + chr4.3:6448748-6448767 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 6448092 6449405 6448092 ID=MS.gene048769
chr4.3 mRNA 6448092 6449405 6448092 ID=MS.gene048769.t1;Parent=MS.gene048769
chr4.3 exon 6448092 6449405 6448092 ID=MS.gene048769.t1.exon1;Parent=MS.gene048769.t1
chr4.3 CDS 6448092 6449405 6448092 ID=cds.MS.gene048769.t1;Parent=MS.gene048769.t1
Gene Sequence

>MS.gene048769

ATGAACGCACTTTCCTTTCTAACCCTTTTTTTCTTTTCCCTTTGTTTCATTGCTTCTTTTTCTCATGCGCTAAATAATGGTTTTAGTGTTGAACTCATCCACCGTGACTCTTCAAAATCACCATTCTACCAACCTACACAAAACAAGTACCAACAAGTTGTCAATGCCGCACGTCGTTCTATCAACCGTGTTAATCATTTCTACAAAAATTCAATTACAAGTACACCTGAATCAACTGTAATTCCTGATAATGGTGAGTATCTCATGACCTATTCAATTGGCAGCCCACCATTTAAGATATATGGCATTGTTGATACAGGTAGTGACATTGTTTGGCTTCAATGCGAGCCTTGTGAAAAATGTTACAACCAAACCACTCCTAAGTTTAAACCATCAAAATCATCAACTTACAAAAATATCCCATGTTCATCTAACCTATGTCAATCTGTGAGAGATATCTCTTGTAATGCCCAAAATTTTTGTGAATATACTATTGCTTATGGAGATCATTCACATTCACAAGGAGATCTTAGTGTTGATACTCTTACATTAGAGTCCTCTACTGGCGGTCATATTTCATTTCCTAAAACTGTGATAGGATGTGGAACCGACAATACAATGTCGTTTAAAGGCGCAAGCTCTGGTATAGTTGGCCTTGGAGGTGGTCCCGTGTCTCTTATAACACAACTTGGATCGTCAATTGGTTCAAAATTCTCTTATTGTTTGCTTCCATTGTCTTTTGAGTCAAACTCAATTGAGTCAAACAAGACAAGCAAACTCAGTTTTGGAGATGCCGCTGTGGTTTCTGGTGATGATGTTGTGTCAACTCCTATAGTGAAAAAACACCCCATAGTTTTCTACTATTTAACATTGGAAGCATTTAGTGTTGGAAACAAAAGAATAGAATTTGGCAGATCATCAAATGGTGGTGATGAGGGTAACATCATAATTGATTCAGGTACAACATTGACGATATTGCCATCTAATATTTATAATAATTTGGAATCAGCCGTGGTAGAATCGGTGAAACTCGAGCGTGTTGATGATCCAACTCAACAATTAAACCTTTGCTATAGTACCACATCAGACGGATATAATTTTCCTCTAATAACAGCGCATTTTAAAGGTGCGGATATTAAGTTGCATCCTATTAGTACCTTTGTTTCCATTGCCAATGGCATTGTTTGCTTTGCTTTTACCGCTTCTTCGGGCGTTGCTATCTTCGGAAACTTAGCTCAGCAGAATTTGTTGGTTGGTTATGACCTTAAACAAAATACTGTGTCATTTAAGCCTACTGATTGTAGCAAAGTGTAA
Protein sequence

>MS.gene048769.t1

MNALSFLTLFFFSLCFIASFSHALNNGFSVELIHRDSSKSPFYQPTQNKYQQVVNAARRSINRVNHFYKNSITSTPESTVIPDNGEYLMTYSIGSPPFKIYGIVDTGSDIVWLQCEPCEKCYNQTTPKFKPSKSSTYKNIPCSSNLCQSVRDISCNAQNFCEYTIAYGDHSHSQGDLSVDTLTLESSTGGHISFPKTVIGCGTDNTMSFKGASSGIVGLGGGPVSLITQLGSSIGSKFSYCLLPLSFESNSIESNKTSKLSFGDAAVVSGDDVVSTPIVKKHPIVFYYLTLEAFSVGNKRIEFGRSSNGGDEGNIIIDSGTTLTILPSNIYNNLESAVVESVKLERVDDPTQQLNLCYSTTSDGYNFPLITAHFKGADIKLHPISTFVSIANGIVCFAFTASSGVAIFGNLAQQNLLVGYDLKQNTVSFKPTDCSKV