AlfalfaGEDB | MS.gene049125

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049125.t1	AET04117.1	96.8	62	1	1	29	89	41	102	1.20E-25	125.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049125.t1	G7LH58	96.8	62	1	1	29	89	41	102	8.6e-26	125.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049125	MS.gene05920	0.848325	6.59E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049125.t1	MTR_8g083450	94.444	90	2	3	3	89	13	102	5.33e-52	158
MS.gene049125.t1	MTR_6g086485	55.435	92	34	4	1	89	1	88	3.31e-22	82.4
MS.gene049125.t1	MTR_5g032790	50.000	58	17	2	33	87	26	74	6.19e-11	53.5

Find 22 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCCGATTCCACTGTCACTTT+TGG	0.208493	4.1:+2679830	None:intergenic
CGACACATGAAGGCTCTTTC+AGG	0.221924	4.1:-2679761	MS.gene049125:CDS
GAACATAGACACCGTCCTTT+GGG	0.403670	4.1:+2679789	None:intergenic
ATGATTAGGAGGCATGGATT+TGG	0.406829	4.1:+2679677	None:intergenic
TGAACATAGACACCGTCCTT+TGG	0.409239	4.1:+2679788	None:intergenic
TTAATTTGAGGTTGAGATTG+AGG	0.413522	4.1:+2679876	None:intergenic
GAAACAACAGACTTATGATT+AGG	0.415127	4.1:+2679663	None:intergenic
CACCTCCACCTTGGATGAAA+AGG	0.419918	4.1:-2679701	MS.gene049125:CDS
CCCAAAAGTGACAGTGGAAT+CGG	0.461245	4.1:-2679831	MS.gene049125:CDS
GTTGAGATTGAGGTTGAGAT+TGG	0.469836	4.1:+2679886	None:intergenic
CGTCATCTGCACCTCCACCT+TGG	0.488683	4.1:-2679710	MS.gene049125:CDS
TTGAGATTGAGGTTGAGATT+GGG	0.491587	4.1:+2679887	None:intergenic
AGACTTATGATTAGGAGGCA+TGG	0.556169	4.1:+2679671	None:intergenic
GAAACGATGAGCTTAATTTG+AGG	0.569661	4.1:+2679864	None:intergenic
CAGTGGAATCGGCGATGCAG+CGG	0.571284	4.1:-2679820	MS.gene049125:CDS
TGTTCACCAACGACACATGA+AGG	0.573889	4.1:-2679771	MS.gene049125:CDS
ACAACAGACTTATGATTAGG+AGG	0.591566	4.1:+2679666	None:intergenic
CGGCGTCGTTTCCCAAAGGA+CGG	0.617718	4.1:-2679800	MS.gene049125:CDS
GCAGCGGCGTCGTTTCCCAA+AGG	0.632009	4.1:-2679804	MS.gene049125:CDS
AAAGAGCCTTCATGTGTCGT+TGG	0.632608	4.1:+2679765	None:intergenic
TCATCACCCAAAAGTGACAG+TGG	0.653040	4.1:-2679837	MS.gene049125:CDS
AAACATATAGTAACTCACTG+TGG	0.708803	4.1:+2679633	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAACAACAGACTTATGATT+AGG	+	chr4.1:2679881-2679900	None:intergenic	30.0%
!	TTAATTTGAGGTTGAGATTG+AGG	+	chr4.1:2679668-2679687	None:intergenic	30.0%
	ACAACAGACTTATGATTAGG+AGG	+	chr4.1:2679878-2679897	None:intergenic	35.0%
	TTGAGATTGAGGTTGAGATT+GGG	+	chr4.1:2679657-2679676	None:intergenic	35.0%
!	GAAACGATGAGCTTAATTTG+AGG	+	chr4.1:2679680-2679699	None:intergenic	35.0%
	AGACTTATGATTAGGAGGCA+TGG	+	chr4.1:2679873-2679892	None:intergenic	40.0%
	GTTGAGATTGAGGTTGAGAT+TGG	+	chr4.1:2679658-2679677	None:intergenic	40.0%
!	ATGATTAGGAGGCATGGATT+TGG	+	chr4.1:2679867-2679886	None:intergenic	40.0%
!!	GATTTGGTCCTTTTCATCCA+AGG	+	chr4.1:2679851-2679870	None:intergenic	40.0%
	AAAGAGCCTTCATGTGTCGT+TGG	+	chr4.1:2679779-2679798	None:intergenic	45.0%
	CCCAAAAGTGACAGTGGAAT+CGG	-	chr4.1:2679710-2679729	MS.gene049125:CDS	45.0%
	GAACATAGACACCGTCCTTT+GGG	+	chr4.1:2679755-2679774	None:intergenic	45.0%
	TCATCACCCAAAAGTGACAG+TGG	-	chr4.1:2679704-2679723	MS.gene049125:CDS	45.0%
	TGAACATAGACACCGTCCTT+TGG	+	chr4.1:2679756-2679775	None:intergenic	45.0%
	TGTTCACCAACGACACATGA+AGG	-	chr4.1:2679770-2679789	MS.gene049125:CDS	45.0%
!	TTGGTCCTTTTCATCCAAGG+TGG	+	chr4.1:2679848-2679867	None:intergenic	45.0%
!!!	AGGTGTCGTTTTCATCGATC+AGG	-	chr4.1:2679800-2679819	MS.gene049125:CDS	45.0%
!!!	CCGATTCCACTGTCACTTTT+GGG	+	chr4.1:2679713-2679732	None:intergenic	45.0%
!!!	GGTGTCGTTTTCATCGATCA+GGG	-	chr4.1:2679801-2679820	MS.gene049125:CDS	45.0%
	CACCTCCACCTTGGATGAAA+AGG	-	chr4.1:2679840-2679859	MS.gene049125:CDS	50.0%
	CGACACATGAAGGCTCTTTC+AGG	-	chr4.1:2679780-2679799	MS.gene049125:CDS	50.0%
!	GTCCTTTTCATCCAAGGTGG+AGG	+	chr4.1:2679845-2679864	None:intergenic	50.0%
!!!	GCCGATTCCACTGTCACTTT+TGG	+	chr4.1:2679714-2679733	None:intergenic	50.0%
	CAGTGGAATCGGCGATGCAG+CGG	-	chr4.1:2679721-2679740	MS.gene049125:CDS	60.0%
	CGGCGTCGTTTCCCAAAGGA+CGG	-	chr4.1:2679741-2679760	MS.gene049125:CDS	60.0%
	CGTCATCTGCACCTCCACCT+TGG	-	chr4.1:2679831-2679850	MS.gene049125:CDS	60.0%
	GCAGCGGCGTCGTTTCCCAA+AGG	-	chr4.1:2679737-2679756	MS.gene049125:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	2679647	2679916	2679647	ID=MS.gene049125
chr4.1	mRNA	2679647	2679916	2679647	ID=MS.gene049125.t1;Parent=MS.gene049125
chr4.1	exon	2679647	2679916	2679647	ID=MS.gene049125.t1.exon1;Parent=MS.gene049125.t1
chr4.1	CDS	2679647	2679916	2679647	ID=cds.MS.gene049125.t1;Parent=MS.gene049125.t1

Gene Sequence	>MS.gene049125 ATGGCTTCCCAATCTCAACCTCAATCTCAACCTCAAATTAAGCTCATCGTTTCAACATCATCACCCAAAAGTGACAGTGGAATCGGCGATGCAGCGGCGTCGTTTCCCAAAGGACGGTGTCTATGTTCACCAACGACACATGAAGGCTCTTTCAGGTGTCGTTTTCATCGATCAGGGACATTTTCGTCATCTGCACCTCCACCTTGGATGAAAAGGACCAAATCCATGCCTCCTAATCATAAGTCTGTTGTTTCTGTTTCTCCACAGTGA
Protein sequence	>MS.gene049125.t1 MASQSQPQSQPQIKLIVSTSSPKSDSGIGDAAASFPKGRCLCSPTTHEGSFRCRFHRSGTFSSSAPPPWMKRTKSMPPNHKSVVSVSPQ

M. sativa cultivar XinJiangDaYe / MS.gene049125