AlfalfaGEDB | MS.gene05920

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05920.t1	AET04117.1	96.8	62	1	1	29	89	41	102	1.20E-25	125.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05920.t1	G7LH58	96.8	62	1	1	29	89	41	102	8.6e-26	125.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049125	MS.gene05920	0.848325	6.59E-60	-1.69E-46
MS.gene057173	MS.gene05920	0.815138	1.08E-51	-1.69E-46
MS.gene05920	MS.gene069785	-0.807441	5.05E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05920.t1	MTR_8g083450	94.444	90	2	3	3	89	13	102	5.33e-52	158
MS.gene05920.t1	MTR_6g086485	55.435	92	34	4	1	89	1	88	3.31e-22	82.4
MS.gene05920.t1	MTR_5g032790	50.000	58	17	2	33	87	26	74	6.19e-11	53.5

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGACACATGAAGGGTCTTTC+AGG	0.198905	4.4:-2984245	MS.gene05920:CDS
GCCGATTCCACTGTCACTTT+TGG	0.208493	4.4:+2984314	None:intergenic
GAACATAGACACCGTCCTTT+GGG	0.403670	4.4:+2984273	None:intergenic
ATGATTAGGAGGCATGGATT+TGG	0.406829	4.4:+2984161	None:intergenic
TGAACATAGACACCGTCCTT+TGG	0.409239	4.4:+2984272	None:intergenic
TTAATTTGAGGTTGAGATTG+AGG	0.413522	4.4:+2984360	None:intergenic
GAAACAACAGACTTATGATT+AGG	0.415127	4.4:+2984147	None:intergenic
CACCTCCACCTTGGATGAAA+AGG	0.419918	4.4:-2984185	MS.gene05920:CDS
CCCAAAAGTGACAGTGGAAT+CGG	0.461245	4.4:-2984315	MS.gene05920:CDS
GTTGAGATTGAGGTTGAGAT+TGG	0.469836	4.4:+2984370	None:intergenic
CGTCATCTGCACCTCCACCT+TGG	0.488683	4.4:-2984194	MS.gene05920:CDS
TTGAGATTGAGGTTGAGATT+GGG	0.491587	4.4:+2984371	None:intergenic
TGTTCACCAACGACACATGA+AGG	0.528018	4.4:-2984255	MS.gene05920:CDS
AGACTTATGATTAGGAGGCA+TGG	0.556169	4.4:+2984155	None:intergenic
GAAACGATGAGCTTAATTTG+AGG	0.569661	4.4:+2984348	None:intergenic
CAGTGGAATCGGCGATGCAG+CGG	0.571284	4.4:-2984304	MS.gene05920:CDS
ACAACAGACTTATGATTAGG+AGG	0.591566	4.4:+2984150	None:intergenic
CGGCGTCGTTTCCCAAAGGA+CGG	0.617718	4.4:-2984284	MS.gene05920:CDS
AAAGACCCTTCATGTGTCGT+TGG	0.628957	4.4:+2984249	None:intergenic
GCAGCGGCGTCGTTTCCCAA+AGG	0.632009	4.4:-2984288	MS.gene05920:CDS
GTTCACCAACGACACATGAA+GGG	0.648482	4.4:-2984254	MS.gene05920:CDS
TCATCACCCAAAAGTGACAG+TGG	0.653040	4.4:-2984321	MS.gene05920:CDS
AAACATATAGTAACTCACTG+TGG	0.708803	4.4:+2984117	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	GAAACAACAGACTTATGATT+AGG	+	chr4.4:2984365-2984384	None:intergenic	30.0%
!	TTAATTTGAGGTTGAGATTG+AGG	+	chr4.4:2984152-2984171	None:intergenic	30.0%
	ACAACAGACTTATGATTAGG+AGG	+	chr4.4:2984362-2984381	None:intergenic	35.0%
	TTGAGATTGAGGTTGAGATT+GGG	+	chr4.4:2984141-2984160	None:intergenic	35.0%
!	GAAACGATGAGCTTAATTTG+AGG	+	chr4.4:2984164-2984183	None:intergenic	35.0%
	AGACTTATGATTAGGAGGCA+TGG	+	chr4.4:2984357-2984376	None:intergenic	40.0%
	GTTGAGATTGAGGTTGAGAT+TGG	+	chr4.4:2984142-2984161	None:intergenic	40.0%
!	ATGATTAGGAGGCATGGATT+TGG	+	chr4.4:2984351-2984370	None:intergenic	40.0%
!!	GATTTGGTCCTTTTCATCCA+AGG	+	chr4.4:2984335-2984354	None:intergenic	40.0%
	AAAGACCCTTCATGTGTCGT+TGG	+	chr4.4:2984263-2984282	None:intergenic	45.0%
	CCCAAAAGTGACAGTGGAAT+CGG	-	chr4.4:2984194-2984213	MS.gene05920:CDS	45.0%
	GAACATAGACACCGTCCTTT+GGG	+	chr4.4:2984239-2984258	None:intergenic	45.0%
	GTTCACCAACGACACATGAA+GGG	-	chr4.4:2984255-2984274	MS.gene05920:CDS	45.0%
	TCATCACCCAAAAGTGACAG+TGG	-	chr4.4:2984188-2984207	MS.gene05920:CDS	45.0%
	TGAACATAGACACCGTCCTT+TGG	+	chr4.4:2984240-2984259	None:intergenic	45.0%
	TGTTCACCAACGACACATGA+AGG	-	chr4.4:2984254-2984273	MS.gene05920:CDS	45.0%
!	TTGGTCCTTTTCATCCAAGG+TGG	+	chr4.4:2984332-2984351	None:intergenic	45.0%
!!!	AGGTGTCGTTTTCATCGATC+AGG	-	chr4.4:2984284-2984303	MS.gene05920:CDS	45.0%
!!!	CCGATTCCACTGTCACTTTT+GGG	+	chr4.4:2984197-2984216	None:intergenic	45.0%
!!!	GGTGTCGTTTTCATCGATCA+GGG	-	chr4.4:2984285-2984304	MS.gene05920:CDS	45.0%
	CACCTCCACCTTGGATGAAA+AGG	-	chr4.4:2984324-2984343	MS.gene05920:CDS	50.0%
	CGACACATGAAGGGTCTTTC+AGG	-	chr4.4:2984264-2984283	MS.gene05920:CDS	50.0%
!	GTCCTTTTCATCCAAGGTGG+AGG	+	chr4.4:2984329-2984348	None:intergenic	50.0%
!!!	GCCGATTCCACTGTCACTTT+TGG	+	chr4.4:2984198-2984217	None:intergenic	50.0%
	CAGTGGAATCGGCGATGCAG+CGG	-	chr4.4:2984205-2984224	MS.gene05920:CDS	60.0%
	CGGCGTCGTTTCCCAAAGGA+CGG	-	chr4.4:2984225-2984244	MS.gene05920:CDS	60.0%
	CGTCATCTGCACCTCCACCT+TGG	-	chr4.4:2984315-2984334	MS.gene05920:CDS	60.0%
	GCAGCGGCGTCGTTTCCCAA+AGG	-	chr4.4:2984221-2984240	MS.gene05920:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	2984131	2984400	2984131	ID=MS.gene05920
chr4.4	mRNA	2984131	2984400	2984131	ID=MS.gene05920.t1;Parent=MS.gene05920
chr4.4	exon	2984131	2984400	2984131	ID=MS.gene05920.t1.exon1;Parent=MS.gene05920.t1
chr4.4	CDS	2984131	2984400	2984131	ID=cds.MS.gene05920.t1;Parent=MS.gene05920.t1

Gene Sequence	>MS.gene05920 ATGGCTTCCCAATCTCAACCTCAATCTCAACCTCAAATTAAGCTCATCGTTTCAACATCATCACCCAAAAGTGACAGTGGAATCGGCGATGCAGCGGCGTCGTTTCCCAAAGGACGGTGTCTATGTTCACCAACGACACATGAAGGGTCTTTCAGGTGTCGTTTTCATCGATCAGGGACATTTTCGTCATCTGCACCTCCACCTTGGATGAAAAGGACCAAATCCATGCCTCCTAATCATAAGTCTGTTGTTTCTGTTTCTCCACAGTGA
Protein sequence	>MS.gene05920.t1 MASQSQPQSQPQIKLIVSTSSPKSDSGIGDAAASFPKGRCLCSPTTHEGSFRCRFHRSGTFSSSAPPPWMKRTKSMPPNHKSVVSVSPQ

M. sativa cultivar XinJiangDaYe / MS.gene05920