Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049141.t1 | XP_003602582.1 | 100 | 57 | 0 | 0 | 1 | 57 | 55 | 111 | 1.60E-23 | 117.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049141.t1 | A0L5Z9 | 60.3 | 58 | 22 | 1 | 1 | 57 | 58 | 115 | 1.4e-12 | 72.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049141.t1 | G7JBH3 | 100.0 | 57 | 0 | 0 | 1 | 57 | 55 | 111 | 1.1e-23 | 117.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049141 | MS.gene05937 | 0.822182 | 2.72E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049141.t1 | MTR_3g095900 | 100.000 | 57 | 0 | 0 | 1 | 57 | 55 | 111 | 1.84e-36 | 119 |
| MS.gene049141.t1 | MTR_8g103900 | 98.246 | 57 | 1 | 0 | 1 | 57 | 55 | 111 | 4.27e-36 | 118 |
| MS.gene049141.t1 | MTR_3g062930 | 50.877 | 57 | 28 | 0 | 1 | 57 | 156 | 212 | 7.45e-14 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049141.t1 | AT5G09770 | 87.719 | 57 | 7 | 0 | 1 | 57 | 55 | 111 | 1.36e-31 | 107 |
| MS.gene049141.t1 | AT5G64650 | 87.719 | 57 | 7 | 0 | 1 | 57 | 55 | 111 | 2.04e-31 | 106 |
| MS.gene049141.t1 | AT3G54210 | 49.123 | 57 | 29 | 0 | 1 | 57 | 153 | 209 | 1.97e-12 | 58.9 |
Find 14 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTACAGAACTGGCTTAT+CGG | 0.376930 | 4.4:-2795099 | MS.gene049141:CDS |
| TCACAAGCTGTTTACAGAAC+TGG | 0.397878 | 4.4:-2795107 | MS.gene049141:CDS |
| ATAGAACTGGTGGATATACT+AGG | 0.438895 | 4.4:-2794984 | MS.gene049141:CDS |
| AGCTCGCCTTGCAGCACATA+CGG | 0.485413 | 4.4:+2795159 | None:intergenic |
| AACAGAGATTTACTCAATAT+AGG | 0.495924 | 4.4:+2794918 | None:intergenic |
| GTGGATATACTAGGGTGTTA+CGG | 0.513509 | 4.4:-2794975 | MS.gene049141:CDS |
| ATTTACTCAATATAGGCCAT+TGG | 0.558368 | 4.4:+2794925 | None:intergenic |
| TTACGGACTCGCATTCGAGT+TGG | 0.558431 | 4.4:-2794958 | MS.gene049141:CDS |
| AGTTGGTGATGCTGCACCAA+TGG | 0.577706 | 4.4:-2794941 | MS.gene049141:CDS |
| TAGAACTGGTGGATATACTA+GGG | 0.605628 | 4.4:-2794983 | MS.gene049141:CDS |
| AGGGATCCGTATGTGCTGCA+AGG | 0.620707 | 4.4:-2795165 | MS.gene049141:CDS |
| GTGTTGTGCAGAGATAGAAC+TGG | 0.623312 | 4.4:-2794997 | MS.gene049141:intron |
| TTGTGCAGAGATAGAACTGG+TGG | 0.666108 | 4.4:-2794994 | MS.gene049141:intron |
| CGAGCTGCTAGTTTCGTGCG+AGG | 0.670232 | 4.4:-2795142 | MS.gene049141:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAGAACTGGTGGATATACT+AGG | - | chr4.4:2795106-2795125 | MS.gene049141:CDS | 35.0% |
| ! | TAGAACTGGTGGATATACTA+GGG | - | chr4.4:2795107-2795126 | MS.gene049141:CDS | 35.0% |
| ! | TGTTTACAGAACTGGCTTAT+CGG | - | chr4.4:2794991-2795010 | MS.gene049141:intron | 35.0% |
| CCAATGGCCTATATTGAGTA+AGG | - | chr4.4:2795165-2795184 | MS.gene049141:CDS | 40.0% | |
| TCACAAGCTGTTTACAGAAC+TGG | - | chr4.4:2794983-2795002 | MS.gene049141:CDS | 40.0% | |
| ! | GTGGATATACTAGGGTGTTA+CGG | - | chr4.4:2795115-2795134 | MS.gene049141:CDS | 40.0% |
| GTGTTGTGCAGAGATAGAAC+TGG | - | chr4.4:2795093-2795112 | MS.gene049141:intron | 45.0% | |
| TTGTGCAGAGATAGAACTGG+TGG | - | chr4.4:2795096-2795115 | MS.gene049141:CDS | 45.0% | |
| TTACGGACTCGCATTCGAGT+TGG | - | chr4.4:2795132-2795151 | MS.gene049141:CDS | 50.0% | |
| !! | AGTTGGTGATGCTGCACCAA+TGG | - | chr4.4:2795149-2795168 | MS.gene049141:CDS | 50.0% |
| AGCTCGCCTTGCAGCACATA+CGG | + | chr4.4:2794934-2794953 | None:intergenic | 55.0% | |
| ! | CGAGCTGCTAGTTTCGTGCG+AGG | - | chr4.4:2794948-2794967 | MS.gene049141:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 2794927 | 2795185 | 2794927 | ID=MS.gene049141 |
| chr4.4 | mRNA | 2794927 | 2795185 | 2794927 | ID=MS.gene049141.t1;Parent=MS.gene049141 |
| chr4.4 | exon | 2795094 | 2795185 | 2795094 | ID=MS.gene049141.t1.exon1;Parent=MS.gene049141.t1 |
| chr4.4 | CDS | 2795094 | 2795185 | 2795094 | ID=cds.MS.gene049141.t1;Parent=MS.gene049141.t1 |
| chr4.4 | exon | 2794927 | 2795008 | 2794927 | ID=MS.gene049141.t1.exon2;Parent=MS.gene049141.t1 |
| chr4.4 | CDS | 2794927 | 2795008 | 2794927 | ID=cds.MS.gene049141.t1;Parent=MS.gene049141.t1 |
| Gene Sequence |
| Protein sequence |
