AlfalfaGEDB | MS.gene049158

M. sativa cultivar XinJiangDaYe / MS.gene049158

KEGG: K07766
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049158.t1	XP_003630930.1	98.3	60	1	0	1	60	1	60	5.20E-25	122.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049158.t1	Q9ZU95	65.0	60	20	1	1	60	6	64	7.1e-15	80.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049158.t1	G7LAJ8	98.3	60	1	0	1	60	1	60	3.7e-25	122.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049158	MS.gene049160	0.866111	3.62E-65	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049158.t1	MTR_8g105140	98.333	60	1	0	1	60	1	60	6.44e-38	123
MS.gene049158.t1	MTR_1g032290	81.667	60	11	0	1	60	1	60	3.43e-31	106
MS.gene049158.t1	MTR_3g065330	62.903	62	20	2	1	60	32	92	2.50e-17	71.2
MS.gene049158.t1	MTR_3g065330	62.903	62	20	2	1	60	10	70	6.55e-17	70.5
MS.gene049158.t1	MTR_6g070920	57.377	61	22	3	1	60	72	129	7.10e-16	67.0
MS.gene049158.t1	MTR_6g070920	57.377	61	22	3	1	60	72	129	2.03e-15	67.0
MS.gene049158.t1	MTR_6g070920	57.377	61	22	3	1	60	72	129	2.43e-15	67.0
MS.gene049158.t1	MTR_5g078020	53.846	65	21	3	1	60	10	70	2.36e-14	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049158.t1	AT2G01670	65.000	60	20	1	1	60	6	64	2.11e-21	81.6
MS.gene049158.t1	AT1G14860	68.333	60	18	1	1	60	1	59	2.84e-21	81.3
MS.gene049158.t1	AT1G18300	52.459	61	28	1	1	60	40	100	1.82e-17	72.0
MS.gene049158.t1	AT1G73540	57.377	61	21	2	1	60	41	97	2.25e-16	69.3

Find 17 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTCTTCTTTATATCTATA+AGG	0.288768	4.4:-2165433	None:intergenic
AAGAAGACATAGATGGTAAT+AGG	0.347424	4.4:+2165446	MS.gene049158:CDS
ATGGGTGGCCGCCAAGTTGT+AGG	0.417911	4.4:+2165059	MS.gene049158:CDS
GTTGAAAGATATGGTTTGTT+TGG	0.419854	4.4:+2164998	None:intergenic
TGGTAATAGGAGCAACGAAT+TGG	0.454436	4.4:+2165459	MS.gene049158:CDS
GTTTGTTTGGTATCTCGCTC+TGG	0.459609	4.4:+2165011	MS.gene049158:CDS
AGATATAAAGAAGACATAGA+TGG	0.486650	4.4:+2165439	MS.gene049158:CDS
GTTTGGTATCTCGCTCTGGA+AGG	0.507086	4.4:+2165015	MS.gene049158:CDS
TTTGGTATCTCGCTCTGGAA+GGG	0.527970	4.4:+2165016	MS.gene049158:CDS
TGGGTGGCCGCCAAGTTGTA+GGG	0.544829	4.4:+2165060	MS.gene049158:CDS
GTACTACTCACCCTACAACT+TGG	0.553526	4.4:-2165070	None:intergenic
CAACGAATTGGAAGTACTAG+TGG	0.598559	4.4:+2165471	MS.gene049158:CDS
TCAGAGACTAATGTTACCCA+AGG	0.637240	4.4:+2165510	MS.gene049158:CDS
CTACTCACCCTACAACTTGG+CGG	0.648959	4.4:-2165067	None:intergenic
CAGAGATATAACAACATGGG+TGG	0.667388	4.4:+2165044	MS.gene049158:CDS
TTGCAGAGATATAACAACAT+GGG	0.682172	4.4:+2165041	MS.gene049158:CDS
GTTGCAGAGATATAACAACA+TGG	0.704593	4.4:+2165040	MS.gene049158:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAATGAGTGAATAGTATT+TGG	-	chr4.4:2165117-2165136	None:intergenic	20.0%
!!	ATGTCTTCTTTATATCTATA+AGG	-	chr4.4:2165436-2165455	None:intergenic	20.0%
!	AGATATAAAGAAGACATAGA+TGG	+	chr4.4:2165439-2165458	MS.gene049158:CDS	25.0%
	AAGAAGACATAGATGGTAAT+AGG	+	chr4.4:2165446-2165465	MS.gene049158:CDS	30.0%
	AATATTTGGTAGGTGACATA+TGG	+	chr4.4:2165149-2165168	MS.gene049158:intron	30.0%
	ACGTTGCAAAAATATTTGGT+AGG	+	chr4.4:2165139-2165158	MS.gene049158:intron	30.0%
	GCAGTCAGAAATTGAATAAA+AGG	-	chr4.4:2165095-2165114	None:intergenic	30.0%
	TTCCACGTTGCAAAAATATT+TGG	+	chr4.4:2165135-2165154	MS.gene049158:intron	30.0%
	TTGCAGAGATATAACAACAT+GGG	+	chr4.4:2165041-2165060	MS.gene049158:CDS	30.0%
!!	TACCAAATATTTTTGCAACG+TGG	-	chr4.4:2165140-2165159	None:intergenic	30.0%
	GTTGCAGAGATATAACAACA+TGG	+	chr4.4:2165040-2165059	MS.gene049158:CDS	35.0%
	CAGAGATATAACAACATGGG+TGG	+	chr4.4:2165044-2165063	MS.gene049158:CDS	40.0%
	TGGTAATAGGAGCAACGAAT+TGG	+	chr4.4:2165459-2165478	MS.gene049158:CDS	40.0%
!!	CAACGAATTGGAAGTACTAG+TGG	+	chr4.4:2165471-2165490	MS.gene049158:CDS	40.0%
	GTACTACTCACCCTACAACT+TGG	-	chr4.4:2165073-2165092	None:intergenic	45.0%
	GTTTGTTTGGTATCTCGCTC+TGG	+	chr4.4:2165011-2165030	MS.gene049158:CDS	45.0%
!!	TTTGGTATCTCGCTCTGGAA+GGG	+	chr4.4:2165016-2165035	MS.gene049158:CDS	45.0%
	CTACTCACCCTACAACTTGG+CGG	-	chr4.4:2165070-2165089	None:intergenic	50.0%
!	GTTTGGTATCTCGCTCTGGA+AGG	+	chr4.4:2165015-2165034	MS.gene049158:CDS	50.0%
	ATGGGTGGCCGCCAAGTTGT+AGG	+	chr4.4:2165059-2165078	MS.gene049158:CDS	60.0%
	TGGGTGGCCGCCAAGTTGTA+GGG	+	chr4.4:2165060-2165079	MS.gene049158:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	2165008	2165531	2165008	ID=MS.gene049158
chr4.4	mRNA	2165008	2165531	2165008	ID=MS.gene049158.t1;Parent=MS.gene049158
chr4.4	exon	2165008	2165081	2165008	ID=MS.gene049158.t1.exon1;Parent=MS.gene049158.t1
chr4.4	CDS	2165008	2165081	2165008	ID=cds.MS.gene049158.t1;Parent=MS.gene049158.t1
chr4.4	exon	2165426	2165531	2165426	ID=MS.gene049158.t1.exon2;Parent=MS.gene049158.t1
chr4.4	CDS	2165426	2165531	2165426	ID=cds.MS.gene049158.t1;Parent=MS.gene049158.t1

Gene Sequence

>MS.gene049158

ATGGTTTGTTTGGTATCTCGCTCTGGAAGGGAGTTGCAGAGATATAACAACATGGGTGGCCGCCAAGTTGTAGGGTGAGTAGTACCTTTTATTCAATTTCTGACTGCCAAATACTATTCACTCATTTTTCCACGTTGCAAAAATATTTGGTAGGTGACATATGGCTCATATACAGTATTGACTTTTTTTACGAAAAAAGTTGATCAGTAACTGATTCTATATATCACGTATTTAATCATCTCTTTAGCTCAATTCACTATTAAAGAACTATAAATTAGTGACAAAATAATTTAAATAATTTTCAGTATAGTTACTCTAATCATTAATTGTATGTATATATCTATTGCATTGTTTGTTCTATTGTATGTTTGTTTCATAATATTGATATATATAATTTATATTGATAATGTTTGCGCAGATGCATACCTTATAGATATAAAGAAGACATAGATGGTAATAGGAGCAACGAATTGGAAGTACTAGTGGTTAGTTCGCAGAAAAGTCAGAGACTAATGTTACCCAAG

Protein sequence

>MS.gene049158.t1

MVCLVSRSGRELQRYNNMGGRQVVGCIPYRYKEDIDGNRSNELEVLVVSSQKSQRLMLPK