Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049160.t1 | XP_003630930.1 | 100 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 3.90E-91 | 344 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049160.t1 | Q9ZU95 | 68.6 | 159 | 49 | 1 | 1 | 159 | 6 | 163 | 8.9e-60 | 231.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049160.t1 | G7LAJ8 | 100.0 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 2.8e-91 | 344.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049158 | MS.gene049160 | 0.866111 | 3.62E-65 | -1.69E-46 |
| MS.gene049160 | MS.gene051837 | 0.806837 | 6.78E-50 | -1.69E-46 |
| MS.gene049160 | MS.gene052668 | 0.852875 | 3.46E-61 | -1.69E-46 |
| MS.gene049160 | MS.gene24855 | 0.806434 | 8.24E-50 | -1.69E-46 |
| MS.gene049160 | MS.gene24856 | 0.80493 | 1.70E-49 | -1.69E-46 |
| MS.gene049160 | MS.gene59219 | 0.805268 | 1.45E-49 | -1.69E-46 |
| MS.gene049160 | MS.gene62693 | 0.871618 | 5.96E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049160.t1 | MTR_8g105140 | 100.000 | 164 | 0 | 0 | 1 | 164 | 1 | 164 | 6.55e-121 | 337 |
| MS.gene049160.t1 | MTR_1g032290 | 81.646 | 158 | 29 | 0 | 1 | 158 | 1 | 158 | 2.45e-96 | 275 |
| MS.gene049160.t1 | MTR_3g065330 | 63.804 | 163 | 56 | 2 | 1 | 161 | 10 | 171 | 2.15e-71 | 213 |
| MS.gene049160.t1 | MTR_5g078020 | 61.111 | 162 | 52 | 4 | 1 | 157 | 10 | 165 | 7.01e-64 | 194 |
| MS.gene049160.t1 | MTR_6g070920 | 59.006 | 161 | 62 | 3 | 1 | 160 | 72 | 229 | 3.24e-63 | 194 |
| MS.gene049160.t1 | MTR_3g065330 | 62.963 | 135 | 47 | 2 | 1 | 133 | 32 | 165 | 2.45e-55 | 172 |
| MS.gene049160.t1 | MTR_6g070920 | 57.971 | 138 | 54 | 3 | 1 | 137 | 72 | 206 | 1.27e-51 | 164 |
| MS.gene049160.t1 | MTR_8g039690 | 49.057 | 159 | 71 | 5 | 1 | 151 | 1 | 157 | 1.88e-42 | 139 |
| MS.gene049160.t1 | MTR_7g084380 | 49.375 | 160 | 70 | 5 | 1 | 150 | 1 | 159 | 2.42e-37 | 128 |
| MS.gene049160.t1 | MTR_7g084380 | 51.111 | 135 | 56 | 4 | 26 | 150 | 21 | 155 | 3.41e-32 | 114 |
| MS.gene049160.t1 | MTR_6g070920 | 59.783 | 92 | 33 | 3 | 1 | 91 | 72 | 160 | 1.67e-30 | 108 |
| MS.gene049160.t1 | MTR_1g078390 | 44.615 | 65 | 34 | 2 | 1 | 64 | 1 | 64 | 3.72e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049160.t1 | AT1G14860 | 69.427 | 157 | 47 | 1 | 1 | 157 | 1 | 156 | 1.96e-77 | 228 |
| MS.gene049160.t1 | AT2G01670 | 68.553 | 159 | 49 | 1 | 1 | 159 | 6 | 163 | 2.72e-77 | 228 |
| MS.gene049160.t1 | AT1G18300 | 54.839 | 155 | 69 | 1 | 1 | 154 | 40 | 194 | 8.99e-63 | 192 |
| MS.gene049160.t1 | AT1G73540 | 52.469 | 162 | 72 | 2 | 1 | 161 | 41 | 198 | 2.10e-59 | 183 |
| MS.gene049160.t1 | AT1G12880 | 49.390 | 164 | 69 | 4 | 1 | 151 | 1 | 163 | 1.02e-41 | 138 |
| MS.gene049160.t1 | AT3G26690 | 43.820 | 178 | 85 | 4 | 1 | 164 | 1 | 177 | 1.35e-40 | 135 |
| MS.gene049160.t1 | AT3G26690 | 43.820 | 178 | 85 | 4 | 1 | 164 | 1 | 177 | 1.35e-40 | 135 |
| MS.gene049160.t1 | AT3G12600 | 47.170 | 159 | 74 | 4 | 1 | 150 | 1 | 158 | 2.01e-33 | 117 |
| MS.gene049160.t1 | AT3G12600 | 46.939 | 147 | 68 | 4 | 13 | 150 | 4 | 149 | 7.12e-30 | 107 |
Find 40 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTCTTCTTTATATCTATA+AGG | 0.288768 | 4.4:-2210520 | None:intergenic |
| AAAATGTTAGGAGAAGAATA+TGG | 0.319122 | 4.4:+2211025 | MS.gene049160:CDS |
| TTCATATGACAGTGCGAATT+GGG | 0.322626 | 4.4:+2210907 | MS.gene049160:intron |
| AAGAAGACATAGATGGTAAT+AGG | 0.347424 | 4.4:+2210533 | MS.gene049160:CDS |
| GAAGGAAGCATTAGATATAT+TGG | 0.347737 | 4.4:+2211210 | MS.gene049160:CDS |
| ATTCATATGACAGTGCGAAT+TGG | 0.395799 | 4.4:+2210906 | MS.gene049160:intron |
| ATGGGTGGCCGCCAAGTTGT+AGG | 0.417911 | 4.4:+2210144 | MS.gene049160:CDS |
| GTTGAAAGATATGGTTTGTT+TGG | 0.419854 | 4.4:+2210083 | None:intergenic |
| CTGTGACTCCTGCTTCTTCA+AGG | 0.425939 | 4.4:-2210782 | None:intergenic |
| GAGAAGTTTGTCAGCATTGG+TGG | 0.451878 | 4.4:+2211185 | MS.gene049160:CDS |
| TGGTAATAGGAGCAACGAAT+TGG | 0.454436 | 4.4:+2210546 | MS.gene049160:CDS |
| GTTTGTTTGGTATCTCGCTC+TGG | 0.459609 | 4.4:+2210096 | MS.gene049160:CDS |
| CATGTTCCCTTTGTTTGTGA+AGG | 0.465572 | 4.4:+2210978 | MS.gene049160:CDS |
| TTGTCAGCATTGGTGGATGA+AGG | 0.468481 | 4.4:+2211192 | MS.gene049160:CDS |
| TGTGACTCCTGCTTCTTCAA+GGG | 0.476266 | 4.4:-2210781 | None:intergenic |
| CAAGAGAAGTTTGTCAGCAT+TGG | 0.482593 | 4.4:+2211182 | MS.gene049160:CDS |
| AGATATAAAGAAGACATAGA+TGG | 0.486650 | 4.4:+2210526 | MS.gene049160:CDS |
| AATTTCATTAGCAAAAGATA+TGG | 0.486910 | 4.4:+2210937 | MS.gene049160:CDS |
| ATTGATCTCAACAGGGAGGA+TGG | 0.489262 | 4.4:+2210718 | MS.gene049160:intron |
| GTTTGGTATCTCGCTCTGGA+AGG | 0.507086 | 4.4:+2210100 | MS.gene049160:CDS |
| CTTGAAGAAGCAGGAGTCAC+AGG | 0.513097 | 4.4:+2210783 | MS.gene049160:CDS |
| AAATGTTAGGAGAAGAATAT+GGG | 0.514195 | 4.4:+2211026 | MS.gene049160:CDS |
| GACAGTGCGAATTGGGGCAG+TGG | 0.525400 | 4.4:+2210914 | MS.gene049160:intron |
| TTTGGTATCTCGCTCTGGAA+GGG | 0.527970 | 4.4:+2210101 | MS.gene049160:CDS |
| AGTTGCTCCTTCACAAACAA+AGG | 0.531029 | 4.4:-2210985 | None:intergenic |
| TGGGTGGCCGCCAAGTTGTA+GGG | 0.544829 | 4.4:+2210145 | MS.gene049160:CDS |
| AGAGAATCCCTTGAAGAAGC+AGG | 0.547892 | 4.4:+2210774 | MS.gene049160:CDS |
| GTACTACTCACCCTACAACT+TGG | 0.553526 | 4.4:-2210155 | None:intergenic |
| AGATATGGCATATACTATGA+AGG | 0.554822 | 4.4:+2210952 | MS.gene049160:CDS |
| TTGATCTCAACAGGGAGGAT+GGG | 0.565610 | 4.4:+2210719 | MS.gene049160:intron |
| GTTGCTCCTTCACAAACAAA+GGG | 0.573725 | 4.4:-2210984 | None:intergenic |
| CAACGAATTGGAAGTACTAG+TGG | 0.598559 | 4.4:+2210558 | MS.gene049160:CDS |
| TCAGAGACTAATGTTTCCCA+AGG | 0.618632 | 4.4:+2210597 | MS.gene049160:CDS |
| CTACTCACCCTACAACTTGG+CGG | 0.620694 | 4.4:-2210152 | None:intergenic |
| TCATATGACAGTGCGAATTG+GGG | 0.622366 | 4.4:+2210908 | MS.gene049160:intron |
| AGGAGTCACAGGATTAGTTG+AGG | 0.634343 | 4.4:+2210794 | MS.gene049160:CDS |
| TGAAGGAGCAACTTGATCAG+TGG | 0.636553 | 4.4:+2210995 | MS.gene049160:CDS |
| CAGAGATATAACAACATGGG+TGG | 0.667388 | 4.4:+2210129 | MS.gene049160:CDS |
| TTGCAGAGATATAACAACAT+GGG | 0.682172 | 4.4:+2210126 | MS.gene049160:CDS |
| GTTGCAGAGATATAACAACA+TGG | 0.704593 | 4.4:+2210125 | MS.gene049160:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTAATATTCCAATTAA+GGG | - | chr4.4:2210681-2210700 | None:intergenic | 10.0% |
| !! | GTTAATTAATATTCCAATTA+AGG | - | chr4.4:2210682-2210701 | None:intergenic | 15.0% |
| !! | TAATTAATATTCCAATTAAG+GGG | - | chr4.4:2210680-2210699 | None:intergenic | 15.0% |
| !! | AAAATGAAGTGAATAGTATT+TGG | - | chr4.4:2210201-2210220 | None:intergenic | 20.0% |
| !! | AATTAATATTCCAATTAAGG+GGG | - | chr4.4:2210679-2210698 | None:intergenic | 20.0% |
| !! | AATTTCATTAGCAAAAGATA+TGG | + | chr4.4:2210937-2210956 | MS.gene049160:CDS | 20.0% |
| !! | ATGTCTTCTTTATATCTATA+AGG | - | chr4.4:2210523-2210542 | None:intergenic | 20.0% |
| !! | TGAAATGAATTTGAAAAATG+TGG | - | chr4.4:2210626-2210645 | None:intergenic | 20.0% |
| !! | TTGAGATCAATCAAATATAT+AGG | - | chr4.4:2210709-2210728 | None:intergenic | 20.0% |
| ! | AAAATGTTAGGAGAAGAATA+TGG | + | chr4.4:2211025-2211044 | MS.gene049160:CDS | 25.0% |
| ! | AAATGTTAGGAGAAGAATAT+GGG | + | chr4.4:2211026-2211045 | MS.gene049160:CDS | 25.0% |
| ! | AGATATAAAGAAGACATAGA+TGG | + | chr4.4:2210526-2210545 | MS.gene049160:CDS | 25.0% |
| ! | ATATTTGATTGATCTCAACA+GGG | + | chr4.4:2210711-2210730 | MS.gene049160:intron | 25.0% |
| ! | TATATTTGATTGATCTCAAC+AGG | + | chr4.4:2210710-2210729 | MS.gene049160:intron | 25.0% |
| ! | TTTCACGTTGCAAAAATATT+TGG | + | chr4.4:2210219-2210238 | MS.gene049160:intron | 25.0% |
| AAGAAGACATAGATGGTAAT+AGG | + | chr4.4:2210533-2210552 | MS.gene049160:CDS | 30.0% | |
| AATATTTGGTAGGTGACATA+TGG | + | chr4.4:2210233-2210252 | MS.gene049160:intron | 30.0% | |
| ACGTTGCAAAAATATTTGGT+AGG | + | chr4.4:2210223-2210242 | MS.gene049160:intron | 30.0% | |
| AGATATGGCATATACTATGA+AGG | + | chr4.4:2210952-2210971 | MS.gene049160:CDS | 30.0% | |
| TTGAAAAATGTGGATACCTT+GGG | - | chr4.4:2210616-2210635 | None:intergenic | 30.0% | |
| TTGCAGAGATATAACAACAT+GGG | + | chr4.4:2210126-2210145 | MS.gene049160:CDS | 30.0% | |
| TTTGAAAAATGTGGATACCT+TGG | - | chr4.4:2210617-2210636 | None:intergenic | 30.0% | |
| !! | GAAGGAAGCATTAGATATAT+TGG | + | chr4.4:2211210-2211229 | MS.gene049160:CDS | 30.0% |
| !! | GGATTAGTTGAGGTATTAAT+TGG | + | chr4.4:2210804-2210823 | MS.gene049160:intron | 30.0% |
| ATTCATATGACAGTGCGAAT+TGG | + | chr4.4:2210906-2210925 | MS.gene049160:intron | 35.0% | |
| GTTGCAGAGATATAACAACA+TGG | + | chr4.4:2210125-2210144 | MS.gene049160:CDS | 35.0% | |
| TTCATATGACAGTGCGAATT+GGG | + | chr4.4:2210907-2210926 | MS.gene049160:intron | 35.0% | |
| ! | CTTCTCCTAACATTTTTCTC+AGG | - | chr4.4:2211021-2211040 | None:intergenic | 35.0% |
| !! | ACTTGCTCATTAGTTGCTTT+TGG | + | chr4.4:2210651-2210670 | MS.gene049160:intron | 35.0% |
| AGTGGCCTGAGAAAAATGTT+AGG | + | chr4.4:2211013-2211032 | MS.gene049160:CDS | 40.0% | |
| AGTTGCTCCTTCACAAACAA+AGG | - | chr4.4:2210988-2211007 | None:intergenic | 40.0% | |
| CAAGAGAAGTTTGTCAGCAT+TGG | + | chr4.4:2211182-2211201 | MS.gene049160:CDS | 40.0% | |
| CAGAGATATAACAACATGGG+TGG | + | chr4.4:2210129-2210148 | MS.gene049160:CDS | 40.0% | |
| GTTGCTCCTTCACAAACAAA+GGG | - | chr4.4:2210987-2211006 | None:intergenic | 40.0% | |
| TCAGAGACTAATGTTTCCCA+AGG | + | chr4.4:2210597-2210616 | MS.gene049160:CDS | 40.0% | |
| TCATATGACAGTGCGAATTG+GGG | + | chr4.4:2210908-2210927 | MS.gene049160:intron | 40.0% | |
| TGGTAATAGGAGCAACGAAT+TGG | + | chr4.4:2210546-2210565 | MS.gene049160:CDS | 40.0% | |
| TTTGATTGATCTCAACAGGG+AGG | + | chr4.4:2210714-2210733 | MS.gene049160:intron | 40.0% | |
| ! | CATGTTCCCTTTGTTTGTGA+AGG | + | chr4.4:2210978-2210997 | MS.gene049160:CDS | 40.0% |
| !! | CAACGAATTGGAAGTACTAG+TGG | + | chr4.4:2210558-2210577 | MS.gene049160:CDS | 40.0% |
| AGAGAATCCCTTGAAGAAGC+AGG | + | chr4.4:2210774-2210793 | MS.gene049160:CDS | 45.0% | |
| ATTGATCTCAACAGGGAGGA+TGG | + | chr4.4:2210718-2210737 | MS.gene049160:intron | 45.0% | |
| GTACTACTCACCCTACAACT+TGG | - | chr4.4:2210158-2210177 | None:intergenic | 45.0% | |
| GTTTGTTTGGTATCTCGCTC+TGG | + | chr4.4:2210096-2210115 | MS.gene049160:CDS | 45.0% | |
| TGAAGGAGCAACTTGATCAG+TGG | + | chr4.4:2210995-2211014 | MS.gene049160:CDS | 45.0% | |
| TGTGACTCCTGCTTCTTCAA+GGG | - | chr4.4:2210784-2210803 | None:intergenic | 45.0% | |
| TTGATCTCAACAGGGAGGAT+GGG | + | chr4.4:2210719-2210738 | MS.gene049160:intron | 45.0% | |
| ! | GAGAAGTTTGTCAGCATTGG+TGG | + | chr4.4:2211185-2211204 | MS.gene049160:CDS | 45.0% |
| !! | AGGAGTCACAGGATTAGTTG+AGG | + | chr4.4:2210794-2210813 | MS.gene049160:CDS | 45.0% |
| !! | TTGTCAGCATTGGTGGATGA+AGG | + | chr4.4:2211192-2211211 | MS.gene049160:CDS | 45.0% |
| !! | TTTGGTATCTCGCTCTGGAA+GGG | + | chr4.4:2210101-2210120 | MS.gene049160:CDS | 45.0% |
| !!! | GCTTTTGGAACCCCCTTAAT+TGG | + | chr4.4:2210666-2210685 | MS.gene049160:intron | 45.0% |
| CTACTCACCCTACAACTTGG+CGG | - | chr4.4:2210155-2210174 | None:intergenic | 50.0% | |
| CTGTGACTCCTGCTTCTTCA+AGG | - | chr4.4:2210785-2210804 | None:intergenic | 50.0% | |
| CTTGAAGAAGCAGGAGTCAC+AGG | + | chr4.4:2210783-2210802 | MS.gene049160:CDS | 50.0% | |
| ! | GTTTGGTATCTCGCTCTGGA+AGG | + | chr4.4:2210100-2210119 | MS.gene049160:CDS | 50.0% |
| ATGGGTGGCCGCCAAGTTGT+AGG | + | chr4.4:2210144-2210163 | MS.gene049160:CDS | 60.0% | |
| GACAGTGCGAATTGGGGCAG+TGG | + | chr4.4:2210914-2210933 | MS.gene049160:intron | 60.0% | |
| TGGGTGGCCGCCAAGTTGTA+GGG | + | chr4.4:2210145-2210164 | MS.gene049160:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 2210093 | 2211267 | 2210093 | ID=MS.gene049160 |
| chr4.4 | mRNA | 2210093 | 2211267 | 2210093 | ID=MS.gene049160.t1;Parent=MS.gene049160 |
| chr4.4 | exon | 2210093 | 2210166 | 2210093 | ID=MS.gene049160.t1.exon1;Parent=MS.gene049160.t1 |
| chr4.4 | CDS | 2210093 | 2210166 | 2210093 | ID=cds.MS.gene049160.t1;Parent=MS.gene049160.t1 |
| chr4.4 | exon | 2210513 | 2210618 | 2210513 | ID=MS.gene049160.t1.exon2;Parent=MS.gene049160.t1 |
| chr4.4 | CDS | 2210513 | 2210618 | 2210513 | ID=cds.MS.gene049160.t1;Parent=MS.gene049160.t1 |
| chr4.4 | exon | 2210732 | 2210815 | 2210732 | ID=MS.gene049160.t1.exon3;Parent=MS.gene049160.t1 |
| chr4.4 | CDS | 2210732 | 2210815 | 2210732 | ID=cds.MS.gene049160.t1;Parent=MS.gene049160.t1 |
| chr4.4 | exon | 2210919 | 2211047 | 2210919 | ID=MS.gene049160.t1.exon4;Parent=MS.gene049160.t1 |
| chr4.4 | CDS | 2210919 | 2211047 | 2210919 | ID=cds.MS.gene049160.t1;Parent=MS.gene049160.t1 |
| chr4.4 | exon | 2211166 | 2211267 | 2211166 | ID=MS.gene049160.t1.exon5;Parent=MS.gene049160.t1 |
| chr4.4 | CDS | 2211166 | 2211267 | 2211166 | ID=cds.MS.gene049160.t1;Parent=MS.gene049160.t1 |
| Gene Sequence |
| Protein sequence |
