AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049800.t1 MTR_4g057730 65.385 260 69 5 1 250 289 537 6.10e-102 307
MS.gene049800.t1 MTR_2g095510 56.693 254 101 4 1 250 286 534 6.40e-87 268
MS.gene049800.t1 MTR_1g063120 48.400 250 126 2 1 248 297 545 5.77e-71 227
MS.gene049800.t1 MTR_1g063110 48.000 250 127 3 1 248 293 541 2.18e-70 226
MS.gene049800.t1 MTR_4g057730 58.763 194 59 5 1 184 289 471 2.56e-59 195
MS.gene049800.t1 MTR_1g073180 58.667 150 61 1 101 250 381 529 1.88e-57 191
MS.gene049800.t1 MTR_1g026310 45.455 242 121 6 13 249 319 554 1.09e-56 190
MS.gene049800.t1 MTR_3g088880 39.062 256 139 4 1 248 303 549 6.34e-56 188
MS.gene049800.t1 MTR_3g088870 39.921 253 141 6 1 248 305 551 1.13e-55 187
MS.gene049800.t1 MTR_5g007640 38.722 266 133 4 1 250 331 582 3.06e-53 181
MS.gene049800.t1 MTR_2g105570 38.281 256 149 4 1 250 292 544 7.05e-53 180
MS.gene049800.t1 MTR_1g090737 56.429 140 61 0 109 248 420 559 2.60e-52 179
MS.gene049800.t1 MTR_4g005750 38.281 256 149 3 1 250 285 537 5.30e-51 174
MS.gene049800.t1 MTR_4g128337 39.924 263 127 4 1 247 293 540 8.28e-51 174
MS.gene049800.t1 MTR_8g039720 58.042 143 56 1 107 249 415 553 3.26e-50 173
MS.gene049800.t1 MTR_5g027480 49.660 147 74 0 101 247 401 547 1.00e-48 169
MS.gene049800.t1 MTR_5g027500 49.660 147 74 0 101 247 414 560 2.28e-48 168
MS.gene049800.t1 MTR_4g077900 47.619 147 77 0 101 247 324 470 1.20e-47 164
MS.gene049800.t1 MTR_3g460770 36.462 277 136 7 1 256 285 542 3.51e-47 164
MS.gene049800.t1 MTR_1g073180 59.677 124 49 1 107 230 409 531 2.01e-46 162
MS.gene049800.t1 MTR_4g057730 54.070 172 58 5 1 162 289 449 1.97e-42 150
MS.gene049800.t1 MTR_4g057755 87.671 73 9 0 111 183 46 118 1.15e-40 136
MS.gene049800.t1 MTR_2g095510 43.373 166 85 4 1 162 286 446 1.14e-31 121
MS.gene049800.t1 MTR_3g035130 38.028 142 87 1 101 242 362 502 4.03e-25 103
MS.gene049800.t1 MTR_1569s0010 36.486 148 91 2 101 247 382 527 9.39e-23 97.1
MS.gene049800.t1 MTR_0161s0050 31.788 151 99 1 101 247 393 543 3.60e-21 92.8
MS.gene049800.t1 MTR_3g064420 31.847 157 99 2 101 250 394 549 3.38e-20 89.7
MS.gene049800.t1 MTR_5g076200 31.126 151 100 1 101 247 391 541 7.57e-20 89.0
MS.gene049800.t1 MTR_5g076220 33.333 153 94 2 101 247 391 541 1.35e-19 88.2
MS.gene049800.t1 MTR_8g074120 32.000 150 100 2 101 250 343 490 3.52e-19 86.7
MS.gene049800.t1 MTR_2g044180 34.194 155 95 3 101 250 311 463 3.92e-19 86.7
MS.gene049800.t1 MTR_2g044180 34.194 155 95 3 101 250 402 554 4.23e-19 86.7
MS.gene049800.t1 MTR_0803s0020 34.868 152 95 3 91 241 586 734 4.51e-19 86.7
MS.gene049800.t1 MTR_8g074040 30.667 150 102 2 101 250 432 579 5.77e-19 86.3
MS.gene049800.t1 MTR_8g074040 30.667 150 102 2 101 250 314 461 1.04e-18 85.5
MS.gene049800.t1 MTR_5g008800 33.333 150 98 2 101 250 388 535 1.57e-18 85.1
MS.gene049800.t1 MTR_3g035130 43.478 92 51 1 101 192 362 452 1.00e-17 82.4
MS.gene049800.t1 MTR_4g121600 32.903 155 97 3 101 250 402 554 1.02e-17 82.4
MS.gene049800.t1 MTR_4g013210 30.435 161 101 3 98 247 278 438 2.31e-17 81.3
MS.gene049800.t1 MTR_4g013210 30.435 161 101 3 98 247 237 397 2.64e-17 80.9
MS.gene049800.t1 MTR_4g013210 30.435 161 101 3 98 247 389 549 2.71e-17 81.3
MS.gene049800.t1 MTR_5g008820 31.915 141 94 2 101 241 386 524 3.99e-17 80.9
MS.gene049800.t1 MTR_4g011790 28.571 154 103 2 101 247 438 591 4.74e-17 80.9
MS.gene049800.t1 MTR_4g011790 27.273 154 105 2 101 247 438 591 7.19e-17 80.1
MS.gene049800.t1 MTR_4g011790 27.273 154 105 2 101 247 447 600 7.73e-17 80.1
MS.gene049800.t1 MTR_5g076240 30.719 153 101 2 101 249 391 542 5.39e-16 77.4
MS.gene049800.t1 MTR_3g088890 46.032 63 34 0 157 219 10 72 6.54e-16 71.2
MS.gene049800.t1 MTR_4g013210 27.737 137 88 2 98 223 430 566 6.87e-13 68.2
MS.gene049800.t1 MTR_1054s0020 31.250 160 105 3 97 252 612 770 1.57e-12 67.4
MS.gene049800.t1 MTR_3g084470 30.496 141 76 3 101 219 523 663 1.81e-11 64.3
MS.gene049800.t1 MTR_1g104810 38.158 76 46 1 98 172 426 501 2.95e-11 63.5
MS.gene049800.t1 MTR_1g104810 38.158 76 46 1 98 172 493 568 3.08e-11 63.5
MS.gene049800.t1 MTR_8g083170 32.704 159 73 6 101 248 530 665 4.35e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049800.t1 AT4G05160 54.724 254 106 4 1 250 291 539 8.85e-84 260
MS.gene049800.t1 AT1G20510 60.403 149 59 0 101 249 390 538 2.82e-60 199
MS.gene049800.t1 AT1G20500 45.267 243 126 4 10 249 306 544 7.34e-59 196
MS.gene049800.t1 AT1G20480 42.259 239 129 4 13 247 324 557 7.12e-54 182
MS.gene049800.t1 AT5G63380 39.841 251 142 5 1 247 305 550 8.71e-54 182
MS.gene049800.t1 AT5G38120 42.387 243 130 5 10 249 309 544 1.27e-53 182
MS.gene049800.t1 AT3G21240 36.364 264 136 4 1 247 298 546 2.88e-53 181
MS.gene049800.t1 AT4G19010 38.583 254 142 6 1 247 308 554 6.39e-52 177
MS.gene049800.t1 AT1G65060 35.055 271 136 3 1 250 308 559 2.05e-50 173
MS.gene049800.t1 AT3G21230 48.701 154 79 0 97 250 410 563 2.77e-50 173
MS.gene049800.t1 AT1G51680 36.466 266 139 3 1 250 305 556 4.38e-47 164
MS.gene049800.t1 AT1G62940 50.000 154 77 0 97 250 382 535 2.76e-43 154
MS.gene049800.t1 AT3G21230 48.299 147 70 3 97 243 410 550 2.24e-42 152
MS.gene049800.t1 AT5G38120 41.315 213 115 5 10 219 309 514 6.60e-41 147
MS.gene049800.t1 AT1G51680 36.170 235 120 3 1 219 305 525 3.37e-39 143
MS.gene049800.t1 AT1G20510 66.667 84 28 0 101 184 390 473 5.73e-33 125
MS.gene049800.t1 AT3G48990 40.789 152 89 1 98 249 358 508 1.09e-32 125
MS.gene049800.t1 AT3G21240 33.168 202 103 4 1 185 298 484 2.38e-31 121
MS.gene049800.t1 AT3G21240 33.168 202 103 4 1 185 298 484 2.38e-31 121
MS.gene049800.t1 AT3G21230 56.322 87 38 0 97 183 410 496 2.87e-31 120
MS.gene049800.t1 AT1G51680 34.673 199 100 3 1 183 305 489 1.28e-26 107
MS.gene049800.t1 AT5G38120 38.764 178 99 5 10 184 309 479 1.37e-26 107
MS.gene049800.t1 AT5G38120 38.764 178 99 5 10 184 309 479 1.37e-26 107
MS.gene049800.t1 AT1G77240 34.000 150 98 1 101 249 390 539 7.13e-24 100
MS.gene049800.t1 AT1G21540 34.641 153 96 1 101 249 392 544 1.15e-23 99.8
MS.gene049800.t1 AT1G21530 31.613 155 100 2 101 249 389 543 9.43e-22 94.4
MS.gene049800.t1 AT1G21530 31.613 155 100 2 101 249 387 541 9.67e-22 94.4
MS.gene049800.t1 AT1G65060 30.387 181 94 4 1 164 308 473 1.45e-20 90.9
MS.gene049800.t1 AT1G20510 62.903 62 23 0 101 162 390 451 3.41e-20 89.7
MS.gene049800.t1 AT1G75960 32.895 152 98 3 101 250 390 539 1.49e-18 85.1
MS.gene049800.t1 AT1G68270 29.299 157 100 2 101 247 363 518 3.18e-18 84.0
MS.gene049800.t1 AT1G20560 32.667 150 99 2 101 250 316 463 7.97e-18 82.8
MS.gene049800.t1 AT1G20560 32.667 150 99 2 101 250 394 541 9.84e-18 82.8
MS.gene049800.t1 AT5G16340 30.137 146 99 2 101 244 390 534 2.61e-17 81.3
MS.gene049800.t1 AT1G65880 28.481 158 101 3 101 247 393 549 3.27e-17 81.3
MS.gene049800.t1 AT1G65890 26.752 157 104 2 101 247 393 548 3.73e-17 80.9
MS.gene049800.t1 AT1G66120 30.000 150 101 2 101 247 393 541 5.11e-17 80.5
MS.gene049800.t1 AT3G16170 26.708 161 107 2 98 247 440 600 6.58e-17 80.5
MS.gene049800.t1 AT5G16370 32.414 145 91 3 101 241 390 531 9.18e-17 79.7
MS.gene049800.t1 AT2G17650 28.758 153 106 3 101 252 448 598 1.83e-16 79.0
MS.gene049800.t1 AT1G30520 40.777 103 56 1 101 198 380 482 1.05e-15 76.6
MS.gene049800.t1 AT1G30520 40.777 103 56 1 101 198 380 482 1.05e-15 76.6
MS.gene049800.t1 AT3G16910 30.573 157 98 4 101 250 405 557 1.13e-15 76.6
MS.gene049800.t1 AT1G76290 32.192 146 96 2 104 247 386 530 1.76e-12 67.0
MS.gene049800.t1 AT2G47240 31.737 167 92 6 86 234 453 615 2.42e-11 63.9
MS.gene049800.t1 AT2G47240 31.737 167 92 6 86 234 453 615 2.42e-11 63.9
MS.gene049800.t1 AT2G47240 31.737 167 92 6 86 234 453 615 2.42e-11 63.9
MS.gene049800.t1 AT2G47240 31.737 167 92 6 86 234 394 556 2.43e-11 63.9
MS.gene049800.t1 AT5G27600 38.028 71 43 1 101 170 499 569 6.71e-11 62.4

Find 61 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTATATCTTATATTT+TGG 0.116812 4.2:+45966423 None:intergenic
TCAGCTGGAATGTTGGTTTC+AGG 0.248160 4.2:-45968079 MS.gene049800:CDS
TCAGAAGTTTATTGCTAATC+AGG 0.249995 4.2:-45966727 MS.gene049800:intron
ACGACAGCATCTTGTATTTC+AGG 0.265567 4.2:+45967055 None:intergenic
TTTCTTCATTGAAGAGTATT+GGG 0.309637 4.2:-45968499 MS.gene049800:CDS
ACCTCATGTTACTATTTATC+AGG 0.312959 4.2:-45968422 MS.gene049800:intron
GGTCTGGTGCTGGCTCCTCT+TGG 0.332249 4.2:-45968478 MS.gene049800:CDS
GATCAAATACAAAGGTTATC+AGG 0.342076 4.2:-45967381 MS.gene049800:intron
CACTCCCCTCCAAAACAATT+GGG 0.349096 4.2:-45968018 MS.gene049800:CDS
GTCTGGTGCTGGCTCCTCTT+GGG 0.359041 4.2:-45968477 MS.gene049800:CDS
CCACTCCCCTCCAAAACAAT+TGG 0.360839 4.2:-45968019 MS.gene049800:CDS
AGCGTAGGTATTCGTCATTC+TGG 0.360952 4.2:-45968103 MS.gene049800:CDS
GCCACCGATGATTCTTGCTT+TGG 0.397468 4.2:-45968552 None:intergenic
ACCTCACCAGCCTCGTCATC+AGG 0.398355 4.2:+45966804 None:intergenic
GTTTCTTCATTGAAGAGTAT+TGG 0.413086 4.2:-45968500 MS.gene049800:CDS
TCCTCGGACAGCAAACTGTT+GGG 0.419851 4.2:+45966756 None:intergenic
AATTTACCTTGCATCATGTT+GGG 0.436629 4.2:+45967975 None:intergenic
TCCTCTTGGGAAGGAATTGA+TGG 0.439409 4.2:-45968464 MS.gene049800:CDS
ATTCGTCATTCTGGCTCAGC+TGG 0.439809 4.2:-45968094 MS.gene049800:CDS
TGGGTACATACAGGAGATCT+TGG 0.444644 4.2:-45967455 MS.gene049800:CDS
TTCCTCGGACAGCAAACTGT+TGG 0.447080 4.2:+45966755 None:intergenic
TTCTGGCTCAGCTGGAATGT+TGG 0.451563 4.2:-45968086 MS.gene049800:CDS
AGTGTTCCTAAGACAGCTTC+AGG 0.454588 4.2:-45966483 MS.gene049800:CDS
AAGAGTATTGGGTCTGGTGC+TGG 0.456352 4.2:-45968488 MS.gene049800:CDS
GAACGAACAACATAGGCAAT+TGG 0.465645 4.2:+45966780 None:intergenic
CAATTTACCTTGCATCATGT+TGG 0.472409 4.2:+45967974 None:intergenic
ATCTTGTACTATTAGGGGTA+TGG 0.475615 4.2:-45968166 MS.gene049800:intron
CGACAGCATCTTGTATTTCA+GGG 0.482401 4.2:+45967056 None:intergenic
TCATTGAAGAGTATTGGGTC+TGG 0.485714 4.2:-45968494 MS.gene049800:CDS
TGGCGAAACAGAGTGTGGTT+GGG 0.494845 4.2:-45968532 MS.gene049800:CDS
TACGATCAACAACATAAAGT+TGG 0.524847 4.2:+45967414 None:intergenic
TCCTGATGACGAGGCTGGTG+AGG 0.526657 4.2:-45966805 MS.gene049800:CDS
AGATTTCCTGATGACGAGGC+TGG 0.527482 4.2:-45966810 MS.gene049800:CDS
AAACTTCTGAATTTCTTCCT+CGG 0.530812 4.2:+45966740 None:intergenic
GAATGACGAATACCTACGCT+TGG 0.539411 4.2:+45968106 None:intergenic
AATGAGGGGTGGGTACATAC+AGG 0.542233 4.2:-45967464 MS.gene049800:CDS
AAAACAAACTTTAGACAATG+AGG 0.546586 4.2:-45967480 MS.gene049800:CDS
AGAAGATCTTCAAGTTCTGC+GGG 0.547646 4.2:+45967088 None:intergenic
GGTGCTGGCTCCTCTTGGGA+AGG 0.549750 4.2:-45968473 MS.gene049800:CDS
TTGGCGAAACAGAGTGTGGT+TGG 0.557745 4.2:-45968533 MS.gene049800:CDS
ACCCAACAGTTTGCTGTCCG+AGG 0.559615 4.2:-45966757 MS.gene049800:CDS
CACCATTCAAAAGATTGCGA+AGG 0.561043 4.2:-45966521 MS.gene049800:CDS
AAACAAACTTTAGACAATGA+GGG 0.563086 4.2:-45967479 MS.gene049800:CDS
ACCATTCAAAAGATTGCGAA+GGG 0.569226 4.2:-45966520 MS.gene049800:CDS
AACTTTAGACAATGAGGGGT+GGG 0.570046 4.2:-45967474 MS.gene049800:CDS
CGAAGCTCAAATAGTGAGTG+TGG 0.579940 4.2:-45968053 MS.gene049800:CDS
GTTGGGTGAACGAACAACAT+AGG 0.583728 4.2:+45966773 None:intergenic
CTATAGATTTCCTGATGACG+AGG 0.586638 4.2:-45966814 MS.gene049800:intron
AAGAAGATCTTCAAGTTCTG+CGG 0.597098 4.2:+45967087 None:intergenic
TTCGCCAAAGCAAGAATCAT+CGG 0.605055 4.2:+45968548 None:intergenic
ACTCCCCTCCAAAACAATTG+GGG 0.605738 4.2:-45968017 MS.gene049800:CDS
TCCATCAATTCCTTCCCAAG+AGG 0.606276 4.2:+45968463 None:intergenic
CTCCCCTCCAAAACAATTGG+GGG 0.613445 4.2:-45968016 MS.gene049800:CDS
TCTATCGAAAATCCAAGCGT+AGG 0.620944 4.2:-45968118 MS.gene049800:CDS
AAACTTTAGACAATGAGGGG+TGG 0.625674 4.2:-45967475 MS.gene049800:CDS
AACAAACTTTAGACAATGAG+GGG 0.631532 4.2:-45967478 MS.gene049800:CDS
TGCTTTGGCGAAACAGAGTG+TGG 0.643161 4.2:-45968537 MS.gene049800:CDS
GAGGGTCCCAACATGATGCA+AGG 0.650137 4.2:-45967981 MS.gene049800:intron
AAAGAACTGATCAAATACAA+AGG 0.656643 4.2:-45967389 MS.gene049800:CDS
ACCTGATAAATAGTAACATG+AGG 0.666648 4.2:+45968421 None:intergenic
AGGTTATTATAACAATCCAG+AGG 0.669013 4.2:-45967507 MS.gene049800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATAGAGAAATATCAAT+TGG + chr4.2:45968115-45968134 None:intergenic 15.0%
!! GTTTAAAAATTAGTTGAAAT+TGG + chr4.2:45968418-45968437 None:intergenic 15.0%
!! AATAACCTGAAATATTGTAT+AGG + chr4.2:45967454-45967473 None:intergenic 20.0%
!! ACAATATAATTGTATTCTGT+TGG + chr4.2:45967073-45967092 None:intergenic 20.0%
!! ACCATTAAAACATAAATACA+GGG + chr4.2:45968377-45968396 None:intergenic 20.0%
!! ATTGATTATCTTGTACTATT+AGG - chr4.2:45966800-45966819 MS.gene049800:CDS 20.0%
!! GATCTTAAGAAATATTAATG+AGG + chr4.2:45967767-45967786 None:intergenic 20.0%
!! TTAATATTTCTTAAGATCGA+TGG - chr4.2:45967769-45967788 MS.gene049800:intron 20.0%
!! TTGATTATCTTGTACTATTA+GGG - chr4.2:45966801-45966820 MS.gene049800:CDS 20.0%
!!! AATTAAAGTATCTTGATCAT+TGG - chr4.2:45966693-45966712 MS.gene049800:intron 20.0%
!!! TAATTTTTAAACACGCTTAT+AGG - chr4.2:45968426-45968445 MS.gene049800:CDS 20.0%
!!! TGTTATTTAAAAGCATGAAA+AGG + chr4.2:45967284-45967303 None:intergenic 20.0%
! AAAACAAACTTTAGACAATG+AGG - chr4.2:45967493-45967512 MS.gene049800:CDS 25.0%
! AAACAAACTTTAGACAATGA+GGG - chr4.2:45967494-45967513 MS.gene049800:CDS 25.0%
! AAAGAACTGATCAAATACAA+AGG - chr4.2:45967584-45967603 MS.gene049800:intron 25.0%
! AATCACTTAGAGTTATTTGT+AGG + chr4.2:45967048-45967067 None:intergenic 25.0%
! ACCATATAAATATACTGTGA+TGG - chr4.2:45968073-45968092 MS.gene049800:CDS 25.0%
! ATGTTACTATTTATCAGGTA+AGG - chr4.2:45966556-45966575 MS.gene049800:intron 25.0%
! CACCATTAAAACATAAATAC+AGG + chr4.2:45968378-45968397 None:intergenic 25.0%
! GTATAGGAAAAGTTTCATAT+GGG + chr4.2:45967438-45967457 None:intergenic 25.0%
! TAAAATCGCTATCTTTAATC+CGG + chr4.2:45966659-45966678 None:intergenic 25.0%
! TATATCTCCAACTTTATGAT+TGG - chr4.2:45967399-45967418 MS.gene049800:CDS 25.0%
! TCCATCACAGTATATTTATA+TGG + chr4.2:45968077-45968096 None:intergenic 25.0%
! TGATTATCTTGTACTATTAG+GGG - chr4.2:45966802-45966821 MS.gene049800:CDS 25.0%
! TGTATAGGAAAAGTTTCATA+TGG + chr4.2:45967439-45967458 None:intergenic 25.0%
! TTACATTCCAATCATAAAGT+TGG + chr4.2:45967409-45967428 None:intergenic 25.0%
!! ATATCCTAACTTGTAACAAT+TGG - chr4.2:45968010-45968029 MS.gene049800:CDS 25.0%
!! CTTTTCCTATACAATATTTC+AGG - chr4.2:45967446-45967465 MS.gene049800:CDS 25.0%
!!! ACCCTGTATTTATGTTTTAA+TGG - chr4.2:45968373-45968392 MS.gene049800:intron 25.0%
!!! ATTGTCTAAAGTTTGTTTTG+TGG + chr4.2:45967492-45967511 None:intergenic 25.0%
!!! CTGTATTTATGTTTTAATGG+TGG - chr4.2:45968376-45968395 MS.gene049800:intron 25.0%
!!! TTGTCTCTTTGAAATTAACT+AGG - chr4.2:45967857-45967876 MS.gene049800:intron 25.0%
!!! TTTCTTCATTGAAGAGTATT+GGG - chr4.2:45966474-45966493 MS.gene049800:CDS 25.0%
AAACTTCTGAATTTCTTCCT+CGG + chr4.2:45968236-45968255 None:intergenic 30.0%
AACAAACTTTAGACAATGAG+GGG - chr4.2:45967495-45967514 MS.gene049800:CDS 30.0%
AAGTAAACATAACAGTGACA+AGG + chr4.2:45967698-45967717 None:intergenic 30.0%
AATTTACCTTGCATCATGTT+GGG + chr4.2:45967001-45967020 None:intergenic 30.0%
ACCTCATGTTACTATTTATC+AGG - chr4.2:45966551-45966570 MS.gene049800:intron 30.0%
ACCTGATAAATAGTAACATG+AGG + chr4.2:45966555-45966574 None:intergenic 30.0%
AGGTTATTATAACAATCCAG+AGG - chr4.2:45967466-45967485 MS.gene049800:CDS 30.0%
CAAACCAATTGTTACAAGTT+AGG + chr4.2:45968017-45968036 None:intergenic 30.0%
GATATAAAAACTGCAAGACT+TGG + chr4.2:45967385-45967404 None:intergenic 30.0%
GATCAAATACAAAGGTTATC+AGG - chr4.2:45967592-45967611 MS.gene049800:intron 30.0%
TACGATCAACAACATAAAGT+TGG + chr4.2:45967562-45967581 None:intergenic 30.0%
TCAGAAGTTTATTGCTAATC+AGG - chr4.2:45968246-45968265 MS.gene049800:intron 30.0%
TGTTATGTTTACTTGAGCAT+TGG - chr4.2:45967704-45967723 MS.gene049800:intron 30.0%
TTGATCAAGAGAAGTTTCAT+TGG + chr4.2:45967232-45967251 None:intergenic 30.0%
TTGGAAAGATGATATGGTAT+TGG - chr4.2:45967723-45967742 MS.gene049800:intron 30.0%
! AGTGTTCTTGTTATGCTTTA+AGG - chr4.2:45967099-45967118 MS.gene049800:intron 30.0%
! GTTTCTTCATTGAAGAGTAT+TGG - chr4.2:45966473-45966492 MS.gene049800:CDS 30.0%
! TATCATGCAAGTTTTACTCA+CGG + chr4.2:45967948-45967967 None:intergenic 30.0%
! TGGATTTTCGATAGAAACAA+CGG + chr4.2:45966850-45966869 None:intergenic 30.0%
!! TAAATCATTTTTATCCCAGC+TGG + chr4.2:45966773-45966792 None:intergenic 30.0%
!!! ACGAGGATTATTTTTGACAA+GGG - chr4.2:45967814-45967833 MS.gene049800:intron 30.0%
!!! CTTGGATATTTTGATGAGAT+CGG - chr4.2:45967536-45967555 MS.gene049800:intron 30.0%
!!! TACGAGGATTATTTTTGACA+AGG - chr4.2:45967813-45967832 MS.gene049800:intron 30.0%
AAAGTCTCTGTAGAGTACAA+TGG - chr4.2:45967344-45967363 MS.gene049800:intron 35.0%
ACATAACAGTGACAAGGATT+TGG + chr4.2:45967692-45967711 None:intergenic 35.0%
ACCATTCAAAAGATTGCGAA+GGG - chr4.2:45968453-45968472 MS.gene049800:CDS 35.0%
ACCGACTGATCTTGATAAAA+CGG + chr4.2:45966636-45966655 None:intergenic 35.0%
ATCTTGTACTATTAGGGGTA+TGG - chr4.2:45966807-45966826 MS.gene049800:CDS 35.0%
CAATGACACATTCAGATCAT+TGG + chr4.2:45966730-45966749 None:intergenic 35.0%
CAATTTACCTTGCATCATGT+TGG + chr4.2:45967002-45967021 None:intergenic 35.0%
CATTGCATTGAACCTATTTG+AGG - chr4.2:45966745-45966764 MS.gene049800:CDS 35.0%
GCATGATAGTTTACGTTTGA+GGG - chr4.2:45967960-45967979 MS.gene049800:intron 35.0%
TAATCTTGATCGGACAGTTA+GGG - chr4.2:45966579-45966598 MS.gene049800:intron 35.0%
TCAGGTAAGGTAATCTTGAT+CGG - chr4.2:45966569-45966588 MS.gene049800:intron 35.0%
TGCATGATAGTTTACGTTTG+AGG - chr4.2:45967959-45967978 MS.gene049800:intron 35.0%
! AAGAAGATCTTCAAGTTCTG+CGG + chr4.2:45967889-45967908 None:intergenic 35.0%
! AATTTTCCTGAAGCTGTCTT+AGG + chr4.2:45968499-45968518 None:intergenic 35.0%
! ATCATGCAAGTTTTACTCAC+GGG + chr4.2:45967947-45967966 None:intergenic 35.0%
! CATATTTCCCCCAATTGTTT+TGG + chr4.2:45966967-45966986 None:intergenic 35.0%
! TTTCTTAAGATCGATGGACT+AGG - chr4.2:45967775-45967794 MS.gene049800:intron 35.0%
!! CAAGCTTTAAACTATGTCTG+AGG - chr4.2:45968334-45968353 MS.gene049800:intron 35.0%
!! CTTTTGCTGTAACTTGTAAG+TGG + chr4.2:45968302-45968321 None:intergenic 35.0%
!! TGAGCATTGGAAAGATGATA+TGG - chr4.2:45967717-45967736 MS.gene049800:intron 35.0%
AAACTTTAGACAATGAGGGG+TGG - chr4.2:45967498-45967517 MS.gene049800:CDS 40.0%
AACTTTAGACAATGAGGGGT+GGG - chr4.2:45967499-45967518 MS.gene049800:CDS 40.0%
ACATTCACGAATTCGGACAT+CGG - chr4.2:45966607-45966626 MS.gene049800:intron 40.0%
ACGACAGCATCTTGTATTTC+AGG + chr4.2:45967921-45967940 None:intergenic 40.0%
CACCATTCAAAAGATTGCGA+AGG - chr4.2:45968452-45968471 MS.gene049800:CDS 40.0%
CGACAGCATCTTGTATTTCA+GGG + chr4.2:45967920-45967939 None:intergenic 40.0%
GAAATCATGTTGTGGTTACG+AGG - chr4.2:45967797-45967816 MS.gene049800:intron 40.0%
GAACGAACAACATAGGCAAT+TGG + chr4.2:45968196-45968215 None:intergenic 40.0%
GTAATCTTGATCGGACAGTT+AGG - chr4.2:45966578-45966597 MS.gene049800:intron 40.0%
TCTATCGAAAATCCAAGCGT+AGG - chr4.2:45966855-45966874 MS.gene049800:intron 40.0%
TTTATCAAGATCAGTCGGTC+CGG - chr4.2:45966637-45966656 MS.gene049800:intron 40.0%
! ACCCTTCGCAATCTTTTGAA+TGG + chr4.2:45968457-45968476 None:intergenic 40.0%
! AGAAGATCTTCAAGTTCTGC+GGG + chr4.2:45967888-45967907 None:intergenic 40.0%
! CTATAGATTTCCTGATGACG+AGG - chr4.2:45968159-45968178 MS.gene049800:intron 40.0%
! TGGACTAGGAAATCATGTTG+TGG - chr4.2:45967789-45967808 MS.gene049800:intron 40.0%
!! GCCGTTTTATCAAGATCAGT+CGG - chr4.2:45966632-45966651 MS.gene049800:intron 40.0%
!! TCATTGAAGAGTATTGGGTC+TGG - chr4.2:45966479-45966498 MS.gene049800:CDS 40.0%
!!! AAAGTTTGTTTTGTGGCCTC+TGG + chr4.2:45967485-45967504 None:intergenic 40.0%
!!! ATTTCCCCCAATTGTTTTGG+AGG + chr4.2:45966964-45966983 None:intergenic 40.0%
!!! TTTCCCCCAATTGTTTTGGA+GGG + chr4.2:45966963-45966982 None:intergenic 40.0%
ACTCCCCTCCAAAACAATTG+GGG - chr4.2:45966956-45966975 MS.gene049800:intron 45.0%
AGCGTAGGTATTCGTCATTC+TGG - chr4.2:45966870-45966889 MS.gene049800:intron 45.0%
AGTGTTCCTAAGACAGCTTC+AGG - chr4.2:45968490-45968509 MS.gene049800:CDS 45.0%
CACTCCCCTCCAAAACAATT+GGG - chr4.2:45966955-45966974 MS.gene049800:intron 45.0%
CGAAGCTCAAATAGTGAGTG+TGG - chr4.2:45966920-45966939 MS.gene049800:intron 45.0%
CTCTGTAGAGTACAATGGCT+AGG - chr4.2:45967349-45967368 MS.gene049800:intron 45.0%
GAATGACGAATACCTACGCT+TGG + chr4.2:45966870-45966889 None:intergenic 45.0%
GGAGCACACATTCACGAATT+CGG - chr4.2:45966600-45966619 MS.gene049800:intron 45.0%
GTTGGGTGAACGAACAACAT+AGG + chr4.2:45968203-45968222 None:intergenic 45.0%
TCCATCAATTCCTTCCCAAG+AGG + chr4.2:45966513-45966532 None:intergenic 45.0%
TCCTCTTGGGAAGGAATTGA+TGG - chr4.2:45966509-45966528 MS.gene049800:CDS 45.0%
TGGGTACATACAGGAGATCT+TGG - chr4.2:45967518-45967537 MS.gene049800:intron 45.0%
!! TCAGCTGGAATGTTGGTTTC+AGG - chr4.2:45966894-45966913 MS.gene049800:intron 45.0%
!!! TTCCCCCAATTGTTTTGGAG+GGG + chr4.2:45966962-45966981 None:intergenic 45.0%
AATGAGGGGTGGGTACATAC+AGG - chr4.2:45967509-45967528 MS.gene049800:intron 50.0%
CCACTCCCCTCCAAAACAAT+TGG - chr4.2:45966954-45966973 MS.gene049800:intron 50.0%
CTCCCCTCCAAAACAATTGG+GGG - chr4.2:45966957-45966976 MS.gene049800:intron 50.0%
TCCTCGGACAGCAAACTGTT+GGG + chr4.2:45968220-45968239 None:intergenic 50.0%
TGCTTTGGCGAAACAGAGTG+TGG - chr4.2:45966436-45966455 MS.gene049800:CDS 50.0%
TGGCGAAACAGAGTGTGGTT+GGG - chr4.2:45966441-45966460 MS.gene049800:CDS 50.0%
TGGGGGAAATATGCGTACGA+GGG - chr4.2:45966974-45966993 MS.gene049800:intron 50.0%
TTCCTCGGACAGCAAACTGT+TGG + chr4.2:45968221-45968240 None:intergenic 50.0%
TTGGCGAAACAGAGTGTGGT+TGG - chr4.2:45966440-45966459 MS.gene049800:CDS 50.0%
TTGGGGGAAATATGCGTACG+AGG - chr4.2:45966973-45966992 MS.gene049800:intron 50.0%
! AACCTATTTGAGGCACCAGC+TGG - chr4.2:45966755-45966774 MS.gene049800:CDS 50.0%
! ACCTATTTGAGGCACCAGCT+GGG - chr4.2:45966756-45966775 MS.gene049800:CDS 50.0%
! AGATTTCCTGATGACGAGGC+TGG - chr4.2:45968163-45968182 MS.gene049800:intron 50.0%
!! AAGAGTATTGGGTCTGGTGC+TGG - chr4.2:45966485-45966504 MS.gene049800:CDS 50.0%
!! ATTCGTCATTCTGGCTCAGC+TGG - chr4.2:45966879-45966898 MS.gene049800:intron 50.0%
!! TTCTGGCTCAGCTGGAATGT+TGG - chr4.2:45966887-45966906 MS.gene049800:intron 50.0%
!!! CCAATTGTTTTGGAGGGGAG+TGG + chr4.2:45966957-45966976 None:intergenic 50.0%
GAGGGTCCCAACATGATGCA+AGG - chr4.2:45966992-45967011 MS.gene049800:intron 55.0%
TCCCAGCTGGTGCCTCAAAT+AGG + chr4.2:45966760-45966779 None:intergenic 55.0%
!! ACCCAACAGTTTGCTGTCCG+AGG - chr4.2:45968216-45968235 MS.gene049800:intron 55.0%
ACCTCACCAGCCTCGTCATC+AGG + chr4.2:45968172-45968191 None:intergenic 60.0%
! TCCTGATGACGAGGCTGGTG+AGG - chr4.2:45968168-45968187 MS.gene049800:intron 60.0%
!! GTCTGGTGCTGGCTCCTCTT+GGG - chr4.2:45966496-45966515 MS.gene049800:CDS 60.0%
!! GGTCTGGTGCTGGCTCCTCT+TGG - chr4.2:45966495-45966514 MS.gene049800:CDS 65.0%
!! GGTGCTGGCTCCTCTTGGGA+AGG - chr4.2:45966500-45966519 MS.gene049800:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 45966428 45968567 45966428 ID=MS.gene049800
chr4.2 mRNA 45966428 45968567 45966428 ID=MS.gene049800.t1;Parent=MS.gene049800
chr4.2 exon 45968423 45968567 45968423 ID=MS.gene049800.t1.exon1;Parent=MS.gene049800.t1
chr4.2 CDS 45968423 45968567 45968423 ID=cds.MS.gene049800.t1;Parent=MS.gene049800.t1
chr4.2 exon 45967982 45968173 45967982 ID=MS.gene049800.t1.exon2;Parent=MS.gene049800.t1
chr4.2 CDS 45967982 45968173 45967982 ID=cds.MS.gene049800.t1;Parent=MS.gene049800.t1
chr4.2 exon 45967382 45967527 45967382 ID=MS.gene049800.t1.exon3;Parent=MS.gene049800.t1
chr4.2 CDS 45967382 45967527 45967382 ID=cds.MS.gene049800.t1;Parent=MS.gene049800.t1
chr4.2 exon 45967049 45967116 45967049 ID=MS.gene049800.t1.exon4;Parent=MS.gene049800.t1
chr4.2 CDS 45967049 45967116 45967049 ID=cds.MS.gene049800.t1;Parent=MS.gene049800.t1
chr4.2 exon 45966728 45966830 45966728 ID=MS.gene049800.t1.exon5;Parent=MS.gene049800.t1
chr4.2 CDS 45966728 45966830 45966728 ID=cds.MS.gene049800.t1;Parent=MS.gene049800.t1
chr4.2 exon 45966428 45966547 45966428 ID=MS.gene049800.t1.exon6;Parent=MS.gene049800.t1
chr4.2 CDS 45966428 45966547 45966428 ID=cds.MS.gene049800.t1;Parent=MS.gene049800.t1
Gene Sequence

>MS.gene049800

ATGATTCTTGCTTTGGCGAAACAGAGTGTGGTTGGGAAATATGATGTTTCTTCATTGAAGAGTATTGGGTCTGGTGCTGGCTCCTCTTGGGAAGGAATTGATGGAGAAGTGTGCTCGACGTCTACCTCATGTTACTATTTATCAGGTAAGGTAATCTTGATCGGACAGTTAGGGAGCACACATTCACGAATTCGGACATCGGTAGCCGTTTTATCAAGATCAGTCGGTCCGGATTAAAGATAGCGATTTTAAGTTATAAAACTGAAATTAAAGTATCTTGATCATTGGTAATTTTTTTACCAATGATCTGAATGTGTCATTGCATTGAACCTATTTGAGGCACCAGCTGGGATAAAAATGATTTATTTATTAATTGATTATCTTGTACTATTAGGGGTATGGTATGACAGAAACTTGTGCCGTTGTTTCTATCGAAAATCCAAGCGTAGGTATTCGTCATTCTGGCTCAGCTGGAATGTTGGTTTCAGGAGTCGAAGCTCAAATAGTGAGTGTGGATACACAGAAGCCACTCCCCTCCAAAACAATTGGGGGAAATATGCGTACGAGGGTCCCAACATGATGCAAGGTAAATTGTCAGCACATTCAACATTTACACTCCTACAAATAACTCTAAGTGATTTTCCAACAGAATACAATTATATTGTACATGTAGTGTTCTTGTTATGCTTTAAGGAATTCAGTAAAAAAAATGACTTGCATTGTTGTATATGCTACTTTCTGATATACATCTATTTTTTCTATTAAAAAATTCAACTTCTTAGTGTATTTGTAAATTCTCTACCAATGAAACTTCTCTTGATCAACTAATAATTAGAAACATGAACTTTAAGTTCCTTTTCATGCTTTTAAATAACATTGTAATCATAATGCATTTTATGATCATACTTACTTGTACAAAGTCTCTGTAGAGTACAATGGCTAGGCACTGTTTCTCCAAGTCTTGCAGTTTTTATATCTCCAACTTTATGATTGGAATGTAAATTTTTCCCATATGAAACTTTTCCTATACAATATTTCAGGTTATTATAACAATCCAGAGGCCACAAAACAAACTTTAGACAATGAGGGGTGGGTACATACAGGAGATCTTGGATATTTTGATGAGATCGGCCAACTTTATGTTGTTGATCGTATCAAAGAACTGATCAAATACAAAGGTTATCAGGTATGTAATCTAAAATGATATCGATTGTAGTAACTAAAGTAACATATTTCATCTATTCATTATCTGAAATCTTAGCCAAATCCTTGTCACTGTTATGTTTACTTGAGCATTGGAAAGATGATATGGTATTGGTTGTATTAATCAAATGATCCTCATTAATATTTCTTAAGATCGATGGACTAGGAAATCATGTTGTGGTTACGAGGATTATTTTTGACAAGGGAATTTCACATTATGTAATATTTGTCTCTTTGAAATTAACTAGGTTGCGCCCGCAGAACTTGAAGATCTTCTTGTTTCTCACCCTGAAATACAAGATGCTGTCGTTATCCCGTGAGTAAAACTTGCATGATAGTTTACGTTTGAGGGATGTTACTTACATCAAAAAATTGAAAAATATCCTAACTTGTAACAATTGGTTTGACACAGAGAAAAAAGTAATAAAAAATGAAATCGTACACCATATAAATATACTGTGATGGACAACAAAACTGTTTTCCAATTGATATTTCTCTATTAATAATTCACATTTGAGTTACTTATTGCTATAGATTTCCTGATGACGAGGCTGGTGAGGTTCCAATTGCCTATGTTGTTCGTTCACCCAACAGTTTGCTGTCCGAGGAAGAAATTCAGAAGTTTATTGCTAATCAGGTGAGAAAAAGCTAAGTAACAAGTTTTATCTCCACTTACAAGTTACAGCAAAAGAAACACATTTTGCAAGCTTTAAACTATGTCTGAGGAATGAAACTGTTTTTAACCCTGTATTTATGTTTTAATGGTGGTCAACTTATTCATTTGCCAATTTCAACTAATTTTTAAACACGCTTATAGGTTGCACCATTCAAAAGATTGCGAAGGGTTACATTCATCAACAGTGTTCCTAAGACAGCTTCAGGAAAATTCTCAGAAGAGAGCTTATTGCAAAAGCACGATCCAAAATATAAGATATAA
Protein sequence

>MS.gene049800.t1

MILALAKQSVVGKYDVSSLKSIGSGAGSSWEGIDGEVCSTSTSCYYLSGVWYDRNLCRCFYRKSKRRYSSFWLSWNVGFRSRSSNSECGYTEATPLQNNWGKYAYEGPNMMQGYYNNPEATKQTLDNEGWVHTGDLGYFDEIGQLYVVDRIKELIKYKGYQVAPAELEDLLVSHPEIQDAVVIPFPDDEAGEVPIAYVVRSPNSLLSEEEIQKFIANQVAPFKRLRRVTFINSVPKTASGKFSEESLLQKHDPKYKI