AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050405


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050405.t1 MTR_7g109510 82.119 151 13 2 1 151 1 137 3.26e-74 218
MS.gene050405.t1 MTR_1g087040 51.370 146 61 3 1 140 1 142 4.07e-39 129
MS.gene050405.t1 MTR_4g070860 48.387 93 47 1 10 101 17 109 1.08e-19 80.5
MS.gene050405.t1 MTR_3g117120 53.012 83 39 0 19 101 27 109 1.13e-19 80.5
MS.gene050405.t1 MTR_1g022495 54.217 83 38 0 19 101 27 109 1.70e-17 74.7
MS.gene050405.t1 MTR_8g086020 40.594 101 55 1 10 105 18 118 8.36e-16 70.5
MS.gene050405.t1 MTR_5g015090 52.857 70 24 1 2 62 32 101 6.42e-13 62.8
MS.gene050405.t1 MTR_4g097440 39.823 113 40 3 7 94 45 154 1.06e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050405.t1 AT3G62420 46.711 152 70 3 1 149 1 144 1.04e-34 118
MS.gene050405.t1 AT2G18160 44.118 102 52 1 18 119 27 123 1.08e-20 83.6
MS.gene050405.t1 AT1G75390 38.849 139 72 3 19 149 38 171 1.44e-20 83.2
MS.gene050405.t1 AT4G34590 48.810 84 43 0 19 102 24 107 1.47e-19 80.1
MS.gene050405.t1 AT1G75390 49.254 67 34 0 19 85 38 104 5.38e-12 59.7

Find 41 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCCGAATCAAGCGATTA+AGG 0.283521 7.2:-5718772 MS.gene050405:CDS
GGCGATTGTTGGAGAACATT+AGG 0.296251 7.2:-5718995 MS.gene050405:CDS
CAGATTGGTTCTTGCATTGA+TGG 0.301394 7.2:-5718737 None:intergenic
GGTTCAACGTGTGACGAGTT+CGG 0.348908 7.2:-5719165 MS.gene050405:CDS
GAGCGATGGACGAGAGAAAA+AGG 0.360427 7.2:-5719124 MS.gene050405:CDS
GTTCAACGTGTGACGAGTTC+GGG 0.369000 7.2:-5719164 MS.gene050405:CDS
TGAATCAATTTGAATGGCTT+CGG 0.385700 7.2:-5719186 None:intergenic
GCGATTGTTGGAGAACATTA+GGG 0.409944 7.2:-5718994 MS.gene050405:CDS
CACGGGTTCAGTTGTGGATC+TGG 0.419548 7.2:+5718804 None:intergenic
GTGATCGCCGTGCGGATTCA+CGG 0.422997 7.2:+5719084 None:intergenic
AGTTGTGGATCTGGAATCTT+CGG 0.445106 7.2:+5718813 None:intergenic
AAACGAGAATCGGCGATTGT+TGG 0.446153 7.2:-5719006 MS.gene050405:CDS
AATGCAAACGAAGCCGCTAA+TGG 0.446683 7.2:-5718954 MS.gene050405:CDS
ATCTTCAAGATGAGACAAAC+AGG 0.448219 7.2:-5719034 MS.gene050405:CDS
AACATTAGGGCGAGGGAAGA+AGG 0.454886 7.2:-5718981 MS.gene050405:CDS
ACGTGTGACGAGTTCGGGTT+CGG 0.460336 7.2:-5719159 MS.gene050405:CDS
GACGCCTTAATCGCTTGATT+CGG 0.461919 7.2:+5718768 None:intergenic
AGGCGTCTTCGTCTTCAGAT+TGG 0.488511 7.2:-5718752 MS.gene050405:CDS
TGTCCTCGGAGCTCGGATAC+AGG 0.488969 7.2:-5718931 MS.gene050405:CDS
AAAGCTGAGAGGGAAAAGAA+TGG 0.490064 7.2:-5718852 MS.gene050405:CDS
TCGGGTTCGGACGGTGTGAA+TGG 0.491849 7.2:-5719146 MS.gene050405:CDS
GTGAATCCGCACGGCGATCA+CGG 0.512811 7.2:-5719082 MS.gene050405:CDS
CTAATGGTGTCCTCGGAGCT+CGG 0.524737 7.2:-5718938 MS.gene050405:CDS
TGAATCCGCACGGCGATCAC+GGG 0.531059 7.2:-5719081 MS.gene050405:CDS
ACAGGTTGCGAAACGAGAAT+CGG 0.540937 7.2:-5719016 MS.gene050405:CDS
CCGCACGGCGATCACGGGAG+AGG 0.550454 7.2:-5719076 MS.gene050405:CDS
TGTTGGAGAACATTAGGGCG+AGG 0.554484 7.2:-5718989 MS.gene050405:CDS
ATCTGCCACGGGTTCAGTTG+TGG 0.561631 7.2:+5718798 None:intergenic
AGCTCCGAGGACACCATTAG+CGG 0.564090 7.2:+5718941 None:intergenic
GAAGCCGCTAATGGTGTCCT+CGG 0.572523 7.2:-5718945 MS.gene050405:CDS
TCGGACAAAGCATCTGCCAC+GGG 0.574386 7.2:+5718787 None:intergenic
GTTGGAGAACATTAGGGCGA+GGG 0.586730 7.2:-5718988 MS.gene050405:CDS
GGAGAGGAAGCAGAAACTGA+TGG 0.591146 7.2:-5719060 MS.gene050405:CDS
TTCGGACAAAGCATCTGCCA+CGG 0.597740 7.2:+5718786 None:intergenic
GGACGGTGTGAATGGAGCGA+TGG 0.618123 7.2:-5719138 MS.gene050405:CDS
GTGACGAGTTCGGGTTCGGA+CGG 0.621958 7.2:-5719155 MS.gene050405:CDS
CAGATCCACAACTGAACCCG+TGG 0.655694 7.2:-5718803 MS.gene050405:CDS
TTTCGAACCGTGAATCCGCA+CGG 0.677805 7.2:-5719091 MS.gene050405:CDS
CCTCTCCCGTGATCGCCGTG+CGG 0.684095 7.2:+5719076 None:intergenic
GAGGAAGCAGAAACTGATGG+AGG 0.689697 7.2:-5719057 MS.gene050405:CDS
ATTCCTGTATCCGAGCTCCG+AGG 0.746883 7.2:+5718928 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCTTCAAGATGAGACAAAC+AGG - chr7.2:5718879-5718898 MS.gene050405:CDS 35.0%
AAAGCTGAGAGGGAAAAGAA+TGG - chr7.2:5719061-5719080 MS.gene050405:CDS 40.0%
GCGATTGTTGGAGAACATTA+GGG - chr7.2:5718919-5718938 MS.gene050405:CDS 40.0%
TGAGAGAGAAAAAGCTGAGA+GGG - chr7.2:5719051-5719070 MS.gene050405:CDS 40.0%
TTGTCCGAATCAAGCGATTA+AGG - chr7.2:5719141-5719160 MS.gene050405:CDS 40.0%
! AGTTGTGGATCTGGAATCTT+CGG + chr7.2:5719103-5719122 None:intergenic 40.0%
AAACGAGAATCGGCGATTGT+TGG - chr7.2:5718907-5718926 MS.gene050405:CDS 45.0%
AATGCAAACGAAGCCGCTAA+TGG - chr7.2:5718959-5718978 MS.gene050405:CDS 45.0%
ACAGGTTGCGAAACGAGAAT+CGG - chr7.2:5718897-5718916 MS.gene050405:CDS 45.0%
CTGAGAGAGAAAAAGCTGAG+AGG - chr7.2:5719050-5719069 MS.gene050405:CDS 45.0%
GACGCCTTAATCGCTTGATT+CGG + chr7.2:5719148-5719167 None:intergenic 45.0%
GGCGATTGTTGGAGAACATT+AGG - chr7.2:5718918-5718937 MS.gene050405:CDS 45.0%
AACATTAGGGCGAGGGAAGA+AGG - chr7.2:5718932-5718951 MS.gene050405:CDS 50.0%
AGGCGTCTTCGTCTTCAGAT+TGG - chr7.2:5719161-5719180 MS.gene050405:CDS 50.0%
GAGCGATGGACGAGAGAAAA+AGG - chr7.2:5718789-5718808 MS.gene050405:CDS 50.0%
GAGGAAGCAGAAACTGATGG+AGG - chr7.2:5718856-5718875 MS.gene050405:CDS 50.0%
GGAGAGGAAGCAGAAACTGA+TGG - chr7.2:5718853-5718872 MS.gene050405:CDS 50.0%
GGTTCAACGTGTGACGAGTT+CGG - chr7.2:5718748-5718767 MS.gene050405:CDS 50.0%
GTTCAACGTGTGACGAGTTC+GGG - chr7.2:5718749-5718768 MS.gene050405:CDS 50.0%
GTTGGAGAACATTAGGGCGA+GGG - chr7.2:5718925-5718944 MS.gene050405:CDS 50.0%
TGTTGGAGAACATTAGGGCG+AGG - chr7.2:5718924-5718943 MS.gene050405:CDS 50.0%
TTTCGAACCGTGAATCCGCA+CGG - chr7.2:5718822-5718841 MS.gene050405:CDS 50.0%
! TTCGGACAAAGCATCTGCCA+CGG + chr7.2:5719130-5719149 None:intergenic 50.0%
AGCTCCGAGGACACCATTAG+CGG + chr7.2:5718975-5718994 None:intergenic 55.0%
ATCTGCCACGGGTTCAGTTG+TGG + chr7.2:5719118-5719137 None:intergenic 55.0%
ATTCCTGTATCCGAGCTCCG+AGG + chr7.2:5718988-5719007 None:intergenic 55.0%
CACGGGTTCAGTTGTGGATC+TGG + chr7.2:5719112-5719131 None:intergenic 55.0%
CAGATCCACAACTGAACCCG+TGG - chr7.2:5719110-5719129 MS.gene050405:CDS 55.0%
CTAATGGTGTCCTCGGAGCT+CGG - chr7.2:5718975-5718994 MS.gene050405:CDS 55.0%
GAAGCCGCTAATGGTGTCCT+CGG - chr7.2:5718968-5718987 MS.gene050405:CDS 55.0%
! ACGTGTGACGAGTTCGGGTT+CGG - chr7.2:5718754-5718773 MS.gene050405:CDS 55.0%
! TCGGACAAAGCATCTGCCAC+GGG + chr7.2:5719129-5719148 None:intergenic 55.0%
GGACGGTGTGAATGGAGCGA+TGG - chr7.2:5718775-5718794 MS.gene050405:CDS 60.0%
GTGAATCCGCACGGCGATCA+CGG - chr7.2:5718831-5718850 MS.gene050405:CDS 60.0%
GTGATCGCCGTGCGGATTCA+CGG + chr7.2:5718832-5718851 None:intergenic 60.0%
TCGGGTTCGGACGGTGTGAA+TGG - chr7.2:5718767-5718786 MS.gene050405:CDS 60.0%
TGAATCCGCACGGCGATCAC+GGG - chr7.2:5718832-5718851 MS.gene050405:CDS 60.0%
TGTCCTCGGAGCTCGGATAC+AGG - chr7.2:5718982-5719001 MS.gene050405:CDS 60.0%
! GTGACGAGTTCGGGTTCGGA+CGG - chr7.2:5718758-5718777 MS.gene050405:CDS 60.0%
CCTCTCCCGTGATCGCCGTG+CGG + chr7.2:5718840-5718859 None:intergenic 70.0%
CCGCACGGCGATCACGGGAG+AGG - chr7.2:5718837-5718856 MS.gene050405:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 5718740 5719195 5718740 ID=MS.gene050405
chr7.2 mRNA 5718740 5719195 5718740 ID=MS.gene050405.t1;Parent=MS.gene050405
chr7.2 exon 5718740 5719195 5718740 ID=MS.gene050405.t1.exon1;Parent=MS.gene050405.t1
chr7.2 CDS 5718740 5719195 5718740 ID=cds.MS.gene050405.t1;Parent=MS.gene050405.t1
Gene Sequence

>MS.gene050405

ATGGCTTCGGTTCAACGTGTGACGAGTTCGGGTTCGGACGGTGTGAATGGAGCGATGGACGAGAGAAAAAGGAAGAGAATGATTTCGAACCGTGAATCCGCACGGCGATCACGGGAGAGGAAGCAGAAACTGATGGAGGATCTTCAAGATGAGACAAACAGGTTGCGAAACGAGAATCGGCGATTGTTGGAGAACATTAGGGCGAGGGAAGAAGGTTTCAATGCAAACGAAGCCGCTAATGGTGTCCTCGGAGCTCGGATACAGGAATTGACAGATCAATTGAAGTTTCTGAACTCAATCATTGAAAAAGCTGAGAGAGAAAAAGCTGAGAGGGAAAAGAATGGATTTTCTGTTGAGATTCCGAAGATTCCAGATCCACAACTGAACCCGTGGCAGATGCTTTGTCCGAATCAAGCGATTAAGGCGTCTTCGTCTTCAGATTGGTTCTTGCATTGA

Protein sequence

>MS.gene050405.t1

MASVQRVTSSGSDGVNGAMDERKRKRMISNRESARRSRERKQKLMEDLQDETNRLRNENRRLLENIRAREEGFNANEAANGVLGARIQELTDQLKFLNSIIEKAEREKAEREKNGFSVEIPKIPDPQLNPWQMLCPNQAIKASSSSDWFLH