Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050407.t1 | XP_003625968.1 | 82.1 | 151 | 13 | 2 | 1 | 151 | 1 | 137 | 1.00E-37 | 166.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050407.t1 | Q9LZP8 | 38.8 | 147 | 81 | 2 | 1 | 144 | 1 | 141 | 4.7e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050407.t1 | G7KST5 | 82.1 | 151 | 13 | 2 | 1 | 151 | 1 | 137 | 7.4e-38 | 166.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene050407.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050405 | MS.gene050407 | 0.98506 | 2.08E-162 | -1.69E-46 |
| MS.gene050407 | MS.gene07054 | 0.930267 | 2.10E-93 | -1.69E-46 |
| MS.gene050407 | MS.gene072700 | 0.817347 | 3.46E-52 | -1.69E-46 |
| MS.gene050407 | MS.gene22502 | 0.986291 | 2.67E-166 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050407.t1 | MTR_7g109510 | 82.119 | 151 | 13 | 2 | 1 | 151 | 1 | 137 | 3.26e-74 | 218 |
| MS.gene050407.t1 | MTR_1g087040 | 51.370 | 146 | 61 | 3 | 1 | 140 | 1 | 142 | 4.07e-39 | 129 |
| MS.gene050407.t1 | MTR_4g070860 | 48.387 | 93 | 47 | 1 | 10 | 101 | 17 | 109 | 1.08e-19 | 80.5 |
| MS.gene050407.t1 | MTR_3g117120 | 53.012 | 83 | 39 | 0 | 19 | 101 | 27 | 109 | 1.13e-19 | 80.5 |
| MS.gene050407.t1 | MTR_1g022495 | 54.217 | 83 | 38 | 0 | 19 | 101 | 27 | 109 | 1.70e-17 | 74.7 |
| MS.gene050407.t1 | MTR_8g086020 | 40.594 | 101 | 55 | 1 | 10 | 105 | 18 | 118 | 8.36e-16 | 70.5 |
| MS.gene050407.t1 | MTR_5g015090 | 52.857 | 70 | 24 | 1 | 2 | 62 | 32 | 101 | 6.42e-13 | 62.8 |
| MS.gene050407.t1 | MTR_4g097440 | 39.823 | 113 | 40 | 3 | 7 | 94 | 45 | 154 | 1.06e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050407.t1 | AT3G62420 | 46.711 | 152 | 70 | 3 | 1 | 149 | 1 | 144 | 1.04e-34 | 118 |
| MS.gene050407.t1 | AT2G18160 | 44.118 | 102 | 52 | 1 | 18 | 119 | 27 | 123 | 1.08e-20 | 83.6 |
| MS.gene050407.t1 | AT1G75390 | 38.849 | 139 | 72 | 3 | 19 | 149 | 38 | 171 | 1.44e-20 | 83.2 |
| MS.gene050407.t1 | AT4G34590 | 48.810 | 84 | 43 | 0 | 19 | 102 | 24 | 107 | 1.47e-19 | 80.1 |
| MS.gene050407.t1 | AT1G75390 | 49.254 | 67 | 34 | 0 | 19 | 85 | 38 | 104 | 5.38e-12 | 59.7 |
Find 0 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCTTCAAGATGAGACAAAC+AGG | - | 51828:9463-9482 | MS.gene050407:CDS | 35.0% | |
| AAAGCTGAGAGGGAAAAGAA+TGG | - | 51828:9645-9664 | MS.gene050407:CDS | 40.0% | |
| GCGATTGTTGGAGAACATTA+GGG | - | 51828:9503-9522 | MS.gene050407:CDS | 40.0% | |
| TGAGAGAGAAAAAGCTGAGA+GGG | - | 51828:9635-9654 | MS.gene050407:CDS | 40.0% | |
| TTGTCCGAATCAAGCGATTA+AGG | - | 51828:9725-9744 | MS.gene050407:CDS | 40.0% | |
| ! | AGTTGTGGATCTGGAATCTT+CGG | + | 51828:9687-9706 | None:intergenic | 40.0% |
| AAACGAGAATCGGCGATTGT+TGG | - | 51828:9491-9510 | MS.gene050407:CDS | 45.0% | |
| AATGCAAACGAAGCCGCTAA+TGG | - | 51828:9543-9562 | MS.gene050407:CDS | 45.0% | |
| ACAGGTTGCGAAACGAGAAT+CGG | - | 51828:9481-9500 | MS.gene050407:CDS | 45.0% | |
| CTGAGAGAGAAAAAGCTGAG+AGG | - | 51828:9634-9653 | MS.gene050407:CDS | 45.0% | |
| GACGCCTTAATCGCTTGATT+CGG | + | 51828:9732-9751 | None:intergenic | 45.0% | |
| GGCGATTGTTGGAGAACATT+AGG | - | 51828:9502-9521 | MS.gene050407:CDS | 45.0% | |
| AACATTAGGGCGAGGGAAGA+AGG | - | 51828:9516-9535 | MS.gene050407:CDS | 50.0% | |
| AGGCGTCTTCGTCTTCAGAT+TGG | - | 51828:9745-9764 | MS.gene050407:CDS | 50.0% | |
| GAGCGATGGACGAGAGAAAA+AGG | - | 51828:9373-9392 | MS.gene050407:CDS | 50.0% | |
| GAGGAAGCAGAAACTGATGG+AGG | - | 51828:9440-9459 | MS.gene050407:CDS | 50.0% | |
| GGAGAGGAAGCAGAAACTGA+TGG | - | 51828:9437-9456 | MS.gene050407:CDS | 50.0% | |
| GGTTCAACGTGTGACGAGTT+CGG | - | 51828:9332-9351 | MS.gene050407:CDS | 50.0% | |
| GTTCAACGTGTGACGAGTTC+GGG | - | 51828:9333-9352 | MS.gene050407:CDS | 50.0% | |
| GTTGGAGAACATTAGGGCGA+GGG | - | 51828:9509-9528 | MS.gene050407:CDS | 50.0% | |
| TGTTGGAGAACATTAGGGCG+AGG | - | 51828:9508-9527 | MS.gene050407:CDS | 50.0% | |
| TTTCGAACCGTGAATCCGCA+CGG | - | 51828:9406-9425 | MS.gene050407:CDS | 50.0% | |
| ! | TTCGGACAAAGCATCTGCCA+CGG | + | 51828:9714-9733 | None:intergenic | 50.0% |
| AGCTCCGAGGACACCATTAG+CGG | + | 51828:9559-9578 | None:intergenic | 55.0% | |
| ATCTGCCACGGGTTCAGTTG+TGG | + | 51828:9702-9721 | None:intergenic | 55.0% | |
| ATTCCTGTATCCGAGCTCCG+AGG | + | 51828:9572-9591 | None:intergenic | 55.0% | |
| CACGGGTTCAGTTGTGGATC+TGG | + | 51828:9696-9715 | None:intergenic | 55.0% | |
| CAGATCCACAACTGAACCCG+TGG | - | 51828:9694-9713 | MS.gene050407:CDS | 55.0% | |
| CTAATGGTGTCCTCGGAGCT+CGG | - | 51828:9559-9578 | MS.gene050407:CDS | 55.0% | |
| GAAGCCGCTAATGGTGTCCT+CGG | - | 51828:9552-9571 | MS.gene050407:CDS | 55.0% | |
| ! | ACGTGTGACGAGTTCGGGTT+CGG | - | 51828:9338-9357 | MS.gene050407:CDS | 55.0% |
| ! | TCGGACAAAGCATCTGCCAC+GGG | + | 51828:9713-9732 | None:intergenic | 55.0% |
| GGACGGTGTGAATGGAGCGA+TGG | - | 51828:9359-9378 | MS.gene050407:CDS | 60.0% | |
| GTGAATCCGCACGGCGATCA+CGG | - | 51828:9415-9434 | MS.gene050407:CDS | 60.0% | |
| GTGATCGCCGTGCGGATTCA+CGG | + | 51828:9416-9435 | None:intergenic | 60.0% | |
| TCGGGTTCGGACGGTGTGAA+TGG | - | 51828:9351-9370 | MS.gene050407:CDS | 60.0% | |
| TGAATCCGCACGGCGATCAC+GGG | - | 51828:9416-9435 | MS.gene050407:CDS | 60.0% | |
| TGTCCTCGGAGCTCGGATAC+AGG | - | 51828:9566-9585 | MS.gene050407:CDS | 60.0% | |
| ! | GTGACGAGTTCGGGTTCGGA+CGG | - | 51828:9342-9361 | MS.gene050407:CDS | 60.0% |
| CCTCTCCCGTGATCGCCGTG+CGG | + | 51828:9424-9443 | None:intergenic | 70.0% | |
| CCGCACGGCGATCACGGGAG+AGG | - | 51828:9421-9440 | MS.gene050407:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51828 | gene | 9324 | 9779 | 9324 | ID=MS.gene050407 |
| 51828 | mRNA | 9324 | 9779 | 9324 | ID=MS.gene050407.t1;Parent=MS.gene050407 |
| 51828 | exon | 9324 | 9779 | 9324 | ID=MS.gene050407.t1.exon1;Parent=MS.gene050407.t1 |
| 51828 | CDS | 9324 | 9779 | 9324 | ID=cds.MS.gene050407.t1;Parent=MS.gene050407.t1 |
| Gene Sequence |
| Protein sequence |
