Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050509.t1 | XP_013458275.1 | 96.9 | 161 | 5 | 0 | 6 | 166 | 251 | 411 | 1.90E-46 | 195.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050509.t1 | A0A072USJ1 | 96.9 | 161 | 5 | 0 | 6 | 166 | 251 | 411 | 1.4e-46 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050509 | MS.gene072533 | 0.93529 | 1.07E-96 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050509.t1 | MTR_4g120360 | 98.734 | 158 | 2 | 0 | 9 | 166 | 254 | 411 | 1.97e-109 | 320 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050509.t1 | AT3G16760 | 52.663 | 169 | 60 | 6 | 1 | 166 | 268 | 419 | 1.55e-40 | 143 |
| MS.gene050509.t1 | AT3G16760 | 44.379 | 169 | 55 | 7 | 1 | 166 | 268 | 400 | 3.64e-23 | 95.5 |
Find 65 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATACTTTAGTATCTTATTAA+TGG | 0.238469 | 7.2:+2804211 | MS.gene050509:CDS |
| CCTCCTCCTGCTGGGATATC+AGG | 0.240809 | 7.2:+2803990 | MS.gene050509:CDS |
| AATTTGCTGATGCTATTAAA+TGG | 0.246283 | 7.2:+2804057 | MS.gene050509:CDS |
| CCAACACCAGTTCAAGCTTC+TGG | 0.260664 | 7.2:+2803804 | MS.gene050509:CDS |
| GGGCTTCCACCTCCTCCTGC+TGG | 0.273283 | 7.2:+2803981 | MS.gene050509:CDS |
| ATGCTATTAAATGGCTTTCA+TGG | 0.318577 | 7.2:+2804066 | MS.gene050509:CDS |
| ATCTTCTGAAATTGATGATT+GGG | 0.330063 | 7.2:+2803902 | MS.gene050509:CDS |
| GATGATTGGGGTACTGAGTT+TGG | 0.345895 | 7.2:+2803915 | MS.gene050509:CDS |
| TCATCTGGAGGTGGTGGTGG+TGG | 0.381873 | 7.2:+2803867 | MS.gene050509:CDS |
| CTACCACCACTTGATGGAAA+TGG | 0.389548 | 7.2:-2803735 | None:intergenic |
| TCTGGAGGTGGTGGTGGTGG+AGG | 0.391020 | 7.2:+2803870 | MS.gene050509:CDS |
| CAACACCAGTTCAAGCTTCT+GGG | 0.394009 | 7.2:+2803805 | MS.gene050509:CDS |
| CATCTTCTGAAATTGATGAT+TGG | 0.399843 | 7.2:+2803901 | MS.gene050509:CDS |
| CCAACACTACCACCACTTGA+TGG | 0.411604 | 7.2:-2803741 | None:intergenic |
| AGGGATGGATAATTACAAAC+AGG | 0.417808 | 7.2:+2804031 | MS.gene050509:CDS |
| GGCTTCCACCTCCTCCTGCT+GGG | 0.431491 | 7.2:+2803982 | MS.gene050509:CDS |
| GCTTCTTGTTATAAAGAAGT+TGG | 0.432373 | 7.2:+2804152 | MS.gene050509:CDS |
| CCATCATCTGGAGGTGGTGG+TGG | 0.433402 | 7.2:+2803864 | MS.gene050509:CDS |
| AGGTGGTGGTGGTGGAGGTG+CGG | 0.446270 | 7.2:+2803875 | MS.gene050509:CDS |
| GGGACAACCACTGAGCTTGA+TGG | 0.450240 | 7.2:+2803960 | MS.gene050509:CDS |
| CTTATTCTTCTAGAGAAAGC+TGG | 0.452802 | 7.2:+2804092 | MS.gene050509:CDS |
| ACATCTACCACTCCATCATC+TGG | 0.458456 | 7.2:+2803852 | MS.gene050509:CDS |
| GGACAACCACTGAGCTTGAT+GGG | 0.464015 | 7.2:+2803961 | MS.gene050509:CDS |
| CCAGAAGCTTGAACTGGTGT+TGG | 0.480586 | 7.2:-2803804 | None:intergenic |
| AAAGTATACCTTGGTACAAT+CGG | 0.485485 | 7.2:-2804195 | None:intergenic |
| TTATTCTTCTAGAGAAAGCT+GGG | 0.492606 | 7.2:+2804093 | MS.gene050509:CDS |
| AGCTGGGGATGGTGCTGGTA+CGG | 0.495673 | 7.2:+2804109 | MS.gene050509:CDS |
| CCACCACCACCTCCAGATGA+TGG | 0.495707 | 7.2:-2803864 | None:intergenic |
| GATATCCCAGCAGGAGGAGG+TGG | 0.497382 | 7.2:-2803987 | None:intergenic |
| CCATCAAGTGGTGGTAGTGT+TGG | 0.502799 | 7.2:+2803741 | MS.gene050509:CDS |
| GAGAAAGCTGGGGATGGTGC+TGG | 0.511690 | 7.2:+2804104 | MS.gene050509:CDS |
| TGGTGGTGGTGGAGGTGCGG+CGG | 0.514395 | 7.2:+2803878 | MS.gene050509:CDS |
| TGCTATTAAATGGCTTTCAT+GGG | 0.517180 | 7.2:+2804067 | MS.gene050509:CDS |
| TGGGGTACTGAGTTTGGAGG+AGG | 0.520172 | 7.2:+2803921 | MS.gene050509:CDS |
| TGGGGATGGTGCTGGTACGG+TGG | 0.527253 | 7.2:+2804112 | MS.gene050509:CDS |
| TGGTTGTCCCACCAACATCA+TGG | 0.529915 | 7.2:-2803947 | None:intergenic |
| ACTCCATCATCTGGAGGTGG+TGG | 0.531742 | 7.2:+2803861 | MS.gene050509:CDS |
| GATTGGGGTACTGAGTTTGG+AGG | 0.532492 | 7.2:+2803918 | MS.gene050509:CDS |
| GGATGGTGCTGGTACGGTGG+AGG | 0.534756 | 7.2:+2804115 | MS.gene050509:CDS |
| ATATCAGGTTCTTCTGCTAA+AGG | 0.534928 | 7.2:+2804005 | MS.gene050509:CDS |
| GGTTCTTCTGCTAAAGGCAA+AGG | 0.540494 | 7.2:+2804011 | MS.gene050509:CDS |
| TCCCATCCATTTCCATCAAG+TGG | 0.550610 | 7.2:+2803729 | MS.gene050509:CDS |
| GTTCTTCTGCTAAAGGCAAA+GGG | 0.550732 | 7.2:+2804012 | MS.gene050509:CDS |
| CCTGATATCCCAGCAGGAGG+AGG | 0.553353 | 7.2:-2803990 | None:intergenic |
| CACCACTTGATGGAAATGGA+TGG | 0.559417 | 7.2:-2803731 | None:intergenic |
| ACCACTCCATCATCTGGAGG+TGG | 0.567943 | 7.2:+2803858 | MS.gene050509:CDS |
| CTTCTAGAGAAAGCTGGGGA+TGG | 0.579736 | 7.2:+2804098 | MS.gene050509:CDS |
| GAAGAACCTGATATCCCAGC+AGG | 0.585834 | 7.2:-2803996 | None:intergenic |
| TTCTGCTAAAGGCAAAGGGA+TGG | 0.585920 | 7.2:+2804016 | MS.gene050509:CDS |
| ACCACTTGATGGAAATGGAT+GGG | 0.594133 | 7.2:-2803730 | None:intergenic |
| TAAGATACTAAAGTATACCT+TGG | 0.595524 | 7.2:-2804204 | None:intergenic |
| AACACCAGTTCAAGCTTCTG+GGG | 0.598648 | 7.2:+2803806 | MS.gene050509:CDS |
| GGAGGAAACCATGATGTTGG+TGG | 0.608973 | 7.2:+2803939 | MS.gene050509:CDS |
| GGAGGAGGAAACCATGATGT+TGG | 0.614688 | 7.2:+2803936 | MS.gene050509:CDS |
| GAGGAAACCATGATGTTGGT+GGG | 0.620042 | 7.2:+2803940 | MS.gene050509:CDS |
| TCTTCTGAAATTGATGATTG+GGG | 0.623414 | 7.2:+2803903 | MS.gene050509:CDS |
| CATCCATTTCCATCAAGTGG+TGG | 0.662013 | 7.2:+2803732 | MS.gene050509:CDS |
| ACCACCTCCAGATGATGGAG+TGG | 0.663977 | 7.2:-2803859 | None:intergenic |
| GAACCTGATATCCCAGCAGG+AGG | 0.665419 | 7.2:-2803993 | None:intergenic |
| GGGATGGATAATTACAAACA+GGG | 0.676491 | 7.2:+2804032 | MS.gene050509:CDS |
| ACACCAGTTCAAGCTTCTGG+GGG | 0.679660 | 7.2:+2803807 | MS.gene050509:CDS |
| TCTACCACTCCATCATCTGG+AGG | 0.681710 | 7.2:+2803855 | MS.gene050509:CDS |
| TATTCTTCTAGAGAAAGCTG+GGG | 0.681721 | 7.2:+2804094 | MS.gene050509:CDS |
| AGGGGTGGCCGATTGTACCA+AGG | 0.686545 | 7.2:+2804187 | MS.gene050509:CDS |
| TGGAAGCCCATCAAGCTCAG+TGG | 0.695115 | 7.2:-2803967 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTTTAGTATCTTATTAA+TGG | + | chr7.2:2804211-2804230 | MS.gene050509:CDS | 15.0% |
| ! | TAAGATACTAAAGTATACCT+TGG | - | chr7.2:2804207-2804226 | None:intergenic | 25.0% |
| !! | AAGTTGGAGAGTATAAAAAA+GGG | + | chr7.2:2804168-2804187 | MS.gene050509:CDS | 25.0% |
| !!! | AATTTGCTGATGCTATTAAA+TGG | + | chr7.2:2804057-2804076 | MS.gene050509:CDS | 25.0% |
| !!! | ATCTTCTGAAATTGATGATT+GGG | + | chr7.2:2803902-2803921 | MS.gene050509:CDS | 25.0% |
| AAAGTATACCTTGGTACAAT+CGG | - | chr7.2:2804198-2804217 | None:intergenic | 30.0% | |
| ATGCTATTAAATGGCTTTCA+TGG | + | chr7.2:2804066-2804085 | MS.gene050509:CDS | 30.0% | |
| GATGTGAAAAACATATCCAT+TGG | - | chr7.2:2803837-2803856 | None:intergenic | 30.0% | |
| TGCTATTAAATGGCTTTCAT+GGG | + | chr7.2:2804067-2804086 | MS.gene050509:CDS | 30.0% | |
| ! | AGTTGGAGAGTATAAAAAAG+GGG | + | chr7.2:2804169-2804188 | MS.gene050509:CDS | 30.0% |
| ! | GAAGTTGGAGAGTATAAAAA+AGG | + | chr7.2:2804167-2804186 | MS.gene050509:CDS | 30.0% |
| ! | GCTTCTTGTTATAAAGAAGT+TGG | + | chr7.2:2804152-2804171 | MS.gene050509:CDS | 30.0% |
| ! | TTATTCTTCTAGAGAAAGCT+GGG | + | chr7.2:2804093-2804112 | MS.gene050509:CDS | 30.0% |
| !! | CATCTTCTGAAATTGATGAT+TGG | + | chr7.2:2803901-2803920 | MS.gene050509:CDS | 30.0% |
| !! | TCTTCTGAAATTGATGATTG+GGG | + | chr7.2:2803903-2803922 | MS.gene050509:CDS | 30.0% |
| !!! | GGAATTGATGTTGATTTTAC+TGG | + | chr7.2:2803762-2803781 | MS.gene050509:CDS | 30.0% |
| !!! | TGTTGATTTTACTGGATCTT+CGG | + | chr7.2:2803770-2803789 | MS.gene050509:CDS | 30.0% |
| AGGGATGGATAATTACAAAC+AGG | + | chr7.2:2804031-2804050 | MS.gene050509:CDS | 35.0% | |
| ATATCAGGTTCTTCTGCTAA+AGG | + | chr7.2:2804005-2804024 | MS.gene050509:CDS | 35.0% | |
| GGGATGGATAATTACAAACA+GGG | + | chr7.2:2804032-2804051 | MS.gene050509:CDS | 35.0% | |
| ! | CTTATTCTTCTAGAGAAAGC+TGG | + | chr7.2:2804092-2804111 | MS.gene050509:CDS | 35.0% |
| ! | TATTCTTCTAGAGAAAGCTG+GGG | + | chr7.2:2804094-2804113 | MS.gene050509:CDS | 35.0% |
| ACCACTTGATGGAAATGGAT+GGG | - | chr7.2:2803733-2803752 | None:intergenic | 40.0% | |
| GTTCTTCTGCTAAAGGCAAA+GGG | + | chr7.2:2804012-2804031 | MS.gene050509:CDS | 40.0% | |
| ! | TGGAGAGTATAAAAAAGGGG+TGG | + | chr7.2:2804172-2804191 | MS.gene050509:CDS | 40.0% |
| AACACCAGTTCAAGCTTCTG+GGG | + | chr7.2:2803806-2803825 | MS.gene050509:CDS | 45.0% | |
| ACATCTACCACTCCATCATC+TGG | + | chr7.2:2803852-2803871 | MS.gene050509:CDS | 45.0% | |
| CAACACCAGTTCAAGCTTCT+GGG | + | chr7.2:2803805-2803824 | MS.gene050509:CDS | 45.0% | |
| CACCACTTGATGGAAATGGA+TGG | - | chr7.2:2803734-2803753 | None:intergenic | 45.0% | |
| CATCCATTTCCATCAAGTGG+TGG | + | chr7.2:2803732-2803751 | MS.gene050509:CDS | 45.0% | |
| CTACCACCACTTGATGGAAA+TGG | - | chr7.2:2803738-2803757 | None:intergenic | 45.0% | |
| GAGGAAACCATGATGTTGGT+GGG | + | chr7.2:2803940-2803959 | MS.gene050509:CDS | 45.0% | |
| GGTTCTTCTGCTAAAGGCAA+AGG | + | chr7.2:2804011-2804030 | MS.gene050509:CDS | 45.0% | |
| TCCCATCCATTTCCATCAAG+TGG | + | chr7.2:2803729-2803748 | MS.gene050509:CDS | 45.0% | |
| TTCTGCTAAAGGCAAAGGGA+TGG | + | chr7.2:2804016-2804035 | MS.gene050509:CDS | 45.0% | |
| !! | GATGATTGGGGTACTGAGTT+TGG | + | chr7.2:2803915-2803934 | MS.gene050509:CDS | 45.0% |
| ACACCAGTTCAAGCTTCTGG+GGG | + | chr7.2:2803807-2803826 | MS.gene050509:CDS | 50.0% | |
| CCAACACCAGTTCAAGCTTC+TGG | + | chr7.2:2803804-2803823 | MS.gene050509:CDS | 50.0% | |
| CCAACACTACCACCACTTGA+TGG | - | chr7.2:2803744-2803763 | None:intergenic | 50.0% | |
| CCAGAAGCTTGAACTGGTGT+TGG | - | chr7.2:2803807-2803826 | None:intergenic | 50.0% | |
| GAAGAACCTGATATCCCAGC+AGG | - | chr7.2:2803999-2804018 | None:intergenic | 50.0% | |
| GGACAACCACTGAGCTTGAT+GGG | + | chr7.2:2803961-2803980 | MS.gene050509:CDS | 50.0% | |
| GGAGGAAACCATGATGTTGG+TGG | + | chr7.2:2803939-2803958 | MS.gene050509:CDS | 50.0% | |
| GGAGGAGGAAACCATGATGT+TGG | + | chr7.2:2803936-2803955 | MS.gene050509:CDS | 50.0% | |
| TCTACCACTCCATCATCTGG+AGG | + | chr7.2:2803855-2803874 | MS.gene050509:CDS | 50.0% | |
| TGGTTGTCCCACCAACATCA+TGG | - | chr7.2:2803950-2803969 | None:intergenic | 50.0% | |
| ! | CCATCAAGTGGTGGTAGTGT+TGG | + | chr7.2:2803741-2803760 | MS.gene050509:CDS | 50.0% |
| ! | CTTCTAGAGAAAGCTGGGGA+TGG | + | chr7.2:2804098-2804117 | MS.gene050509:CDS | 50.0% |
| !! | GATTGGGGTACTGAGTTTGG+AGG | + | chr7.2:2803918-2803937 | MS.gene050509:CDS | 50.0% |
| ACCACTCCATCATCTGGAGG+TGG | + | chr7.2:2803858-2803877 | MS.gene050509:CDS | 55.0% | |
| ACTCCATCATCTGGAGGTGG+TGG | + | chr7.2:2803861-2803880 | MS.gene050509:CDS | 55.0% | |
| GAACCTGATATCCCAGCAGG+AGG | - | chr7.2:2803996-2804015 | None:intergenic | 55.0% | |
| GGGACAACCACTGAGCTTGA+TGG | + | chr7.2:2803960-2803979 | MS.gene050509:CDS | 55.0% | |
| TCACCCCCAGAAGCTTGAAC+TGG | - | chr7.2:2803813-2803832 | None:intergenic | 55.0% | |
| TGGAAGCCCATCAAGCTCAG+TGG | - | chr7.2:2803970-2803989 | None:intergenic | 55.0% | |
| ! | AGCTTCTGGGGGTGATCCAA+TGG | + | chr7.2:2803818-2803837 | MS.gene050509:CDS | 55.0% |
| !! | ACCACCTCCAGATGATGGAG+TGG | - | chr7.2:2803862-2803881 | None:intergenic | 55.0% |
| !! | TGGGGTACTGAGTTTGGAGG+AGG | + | chr7.2:2803921-2803940 | MS.gene050509:CDS | 55.0% |
| AGGGGTGGCCGATTGTACCA+AGG | + | chr7.2:2804187-2804206 | MS.gene050509:CDS | 60.0% | |
| CCACCACCACCTCCAGATGA+TGG | - | chr7.2:2803867-2803886 | None:intergenic | 60.0% | |
| CCTCCTCCTGCTGGGATATC+AGG | + | chr7.2:2803990-2804009 | MS.gene050509:CDS | 60.0% | |
| CCTGATATCCCAGCAGGAGG+AGG | - | chr7.2:2803993-2804012 | None:intergenic | 60.0% | |
| GAGAAAGCTGGGGATGGTGC+TGG | + | chr7.2:2804104-2804123 | MS.gene050509:CDS | 60.0% | |
| GATATCCCAGCAGGAGGAGG+TGG | - | chr7.2:2803990-2804009 | None:intergenic | 60.0% | |
| ! | CCATCATCTGGAGGTGGTGG+TGG | + | chr7.2:2803864-2803883 | MS.gene050509:CDS | 60.0% |
| !! | AGCTGGGGATGGTGCTGGTA+CGG | + | chr7.2:2804109-2804128 | MS.gene050509:CDS | 60.0% |
| !! | TCATCTGGAGGTGGTGGTGG+TGG | + | chr7.2:2803867-2803886 | MS.gene050509:CDS | 60.0% |
| GGCTTCCACCTCCTCCTGCT+GGG | + | chr7.2:2803982-2804001 | MS.gene050509:CDS | 65.0% | |
| !! | AGGTGGTGGTGGTGGAGGTG+CGG | + | chr7.2:2803875-2803894 | MS.gene050509:CDS | 65.0% |
| !! | GGATGGTGCTGGTACGGTGG+AGG | + | chr7.2:2804115-2804134 | MS.gene050509:CDS | 65.0% |
| !! | TCTGGAGGTGGTGGTGGTGG+AGG | + | chr7.2:2803870-2803889 | MS.gene050509:CDS | 65.0% |
| !! | TGGGGATGGTGCTGGTACGG+TGG | + | chr7.2:2804112-2804131 | MS.gene050509:CDS | 65.0% |
| GGGCTTCCACCTCCTCCTGC+TGG | + | chr7.2:2803981-2804000 | MS.gene050509:CDS | 70.0% | |
| !! | TGGTGGTGGTGGAGGTGCGG+CGG | + | chr7.2:2803878-2803897 | MS.gene050509:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 2803714 | 2804271 | 2803714 | ID=MS.gene050509 |
| chr7.2 | mRNA | 2803714 | 2804271 | 2803714 | ID=MS.gene050509.t1;Parent=MS.gene050509 |
| chr7.2 | exon | 2803714 | 2804271 | 2803714 | ID=MS.gene050509.t1.exon1;Parent=MS.gene050509.t1 |
| chr7.2 | CDS | 2803714 | 2804271 | 2803714 | ID=cds.MS.gene050509.t1;Parent=MS.gene050509.t1 |
| Gene Sequence |
| Protein sequence |
