Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05060.t1 | XP_003600584.1 | 87.3 | 212 | 27 | 0 | 1 | 212 | 115 | 326 | 2.10E-97 | 365.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05060.t1 | G7J778 | 87.3 | 212 | 27 | 0 | 1 | 212 | 115 | 326 | 1.5e-97 | 365.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05060 | MS.gene55664 | 0.803211 | 3.89E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05060.t1 | MTR_3g063950 | 87.264 | 212 | 27 | 0 | 1 | 212 | 115 | 326 | 1.27e-126 | 360 |
| MS.gene05060.t1 | MTR_3g063940 | 72.381 | 210 | 58 | 0 | 1 | 210 | 120 | 329 | 1.19e-105 | 307 |
| MS.gene05060.t1 | MTR_3g063940 | 71.429 | 140 | 40 | 0 | 1 | 140 | 120 | 259 | 3.13e-65 | 202 |
| MS.gene05060.t1 | MTR_7g114660 | 51.220 | 205 | 70 | 3 | 1 | 205 | 213 | 387 | 9.52e-54 | 177 |
| MS.gene05060.t1 | MTR_8g465010 | 49.756 | 205 | 73 | 3 | 1 | 205 | 208 | 382 | 9.09e-52 | 171 |
| MS.gene05060.t1 | MTR_8g031500 | 60.870 | 69 | 26 | 1 | 137 | 205 | 275 | 342 | 3.08e-17 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05060.t1 | AT1G54520 | 45.854 | 205 | 81 | 4 | 1 | 205 | 214 | 388 | 4.67e-45 | 154 |
Find 44 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTGCAGGTGGATCTTAAA+AGG | 0.274049 | 3.4:-65800781 | MS.gene05060:intron |
| GAAGGGAGCTGAATATTTAA+AGG | 0.344002 | 3.4:-65798482 | MS.gene05060:CDS |
| TTCAGGCTGGCGAGGTACTA+TGG | 0.358063 | 3.4:-65798312 | MS.gene05060:intron |
| GAAGAAAGGGAGAAATTTGA+TGG | 0.359812 | 3.4:-65800716 | MS.gene05060:CDS |
| TATTCAGCTCCCTTCATGTT+AGG | 0.362444 | 3.4:+65798490 | None:intergenic |
| CCCTTTCTTCAATTGATAGT+TGG | 0.365483 | 3.4:+65800729 | None:intergenic |
| CTTATGTTAGGTAACAATAT+TGG | 0.386874 | 3.4:-65798539 | MS.gene05060:intron |
| AGCATGTTTCTCCACCCTCC+TGG | 0.401006 | 3.4:+65800756 | None:intergenic |
| AGCTGCTAATACACGGTATG+AGG | 0.403947 | 3.4:-65810401 | MS.gene05060:CDS |
| TTTGTTTAAGGTTGGAATGT+TGG | 0.409336 | 3.4:-65810470 | MS.gene05060:intron |
| GGGTTTCATCCTCTCTCTGT+GGG | 0.434025 | 3.4:+65798287 | None:intergenic |
| GGTTGGAATGTTGGATGAGA+TGG | 0.439180 | 3.4:-65810461 | MS.gene05060:CDS |
| AACAGAGACAATACAAACTT+TGG | 0.439999 | 3.4:-65808889 | MS.gene05060:intron |
| ACCAACTATCAATTGAAGAA+AGG | 0.462903 | 3.4:-65800730 | MS.gene05060:CDS |
| CCAACTATCAATTGAAGAAA+GGG | 0.475889 | 3.4:-65800729 | MS.gene05060:CDS |
| TTATTCATATTTCATAGACT+TGG | 0.480040 | 3.4:+65798225 | None:intergenic |
| CATCATGGTTACTGTATAGC+AGG | 0.483872 | 3.4:-65808861 | MS.gene05060:CDS |
| CAAACTTTGGATAAACATCA+TGG | 0.498262 | 3.4:-65808876 | MS.gene05060:CDS |
| GATGGGATGTACATTACAAA+GGG | 0.528262 | 3.4:-65810443 | MS.gene05060:CDS |
| ACAATATTGGTAGCTACTAA+AGG | 0.530785 | 3.4:-65798526 | MS.gene05060:CDS |
| CATTACAAAGGGATCTAGCT+AGG | 0.535268 | 3.4:-65810432 | MS.gene05060:CDS |
| TTCATAGACTTGGCTAGCTG+AGG | 0.537152 | 3.4:+65798235 | None:intergenic |
| GAGGATGAAACCCTTTCAGA+TGG | 0.539392 | 3.4:-65798277 | MS.gene05060:CDS |
| TGCTAAACCGATCATAACTC+TGG | 0.546715 | 3.4:+65800645 | None:intergenic |
| GTTGGAATGTTGGATGAGAT+GGG | 0.546768 | 3.4:-65810460 | MS.gene05060:CDS |
| GGTGGATCTTAAAAGGAGCC+AGG | 0.555856 | 3.4:-65800774 | MS.gene05060:CDS |
| TCATAGACTTGGCTAGCTGA+GGG | 0.559467 | 3.4:+65798236 | None:intergenic |
| GAAATTCTTTCAGGCTGGCG+AGG | 0.569656 | 3.4:-65798320 | MS.gene05060:intron |
| AGGGTTTCATCCTCTCTCTG+TGG | 0.571271 | 3.4:+65798286 | None:intergenic |
| ACATAAGCTACCTAACATGA+AGG | 0.574557 | 3.4:-65798500 | MS.gene05060:CDS |
| AGATGGGATGTACATTACAA+AGG | 0.592518 | 3.4:-65810444 | MS.gene05060:CDS |
| GATCTTAAAAGGAGCCAGGA+GGG | 0.605501 | 3.4:-65800770 | MS.gene05060:CDS |
| ACTATGGACCCCACAGAGAG+AGG | 0.608037 | 3.4:-65798296 | MS.gene05060:CDS |
| GCTGCTAATACACGGTATGA+GGG | 0.609541 | 3.4:-65810400 | MS.gene05060:CDS |
| GTTTAGCAATGAATATTCCA+CGG | 0.610272 | 3.4:-65800630 | MS.gene05060:intron |
| CTTAAAAGGAGCCAGGAGGG+TGG | 0.619800 | 3.4:-65800767 | MS.gene05060:CDS |
| TAAGAAGCCAGAGTTATGAT+CGG | 0.619942 | 3.4:-65800652 | MS.gene05060:CDS |
| TTGCAGAAGCTGCTAATACA+CGG | 0.637222 | 3.4:-65810408 | MS.gene05060:CDS |
| GGTTTCATCCTCTCTCTGTG+GGG | 0.650477 | 3.4:+65798288 | None:intergenic |
| GGCGTGTGCTGTTTATTGAG+AGG | 0.655354 | 3.4:-65810376 | MS.gene05060:intron |
| CATAAGCTACCTAACATGAA+GGG | 0.659099 | 3.4:-65798499 | MS.gene05060:CDS |
| GCTAATACACGGTATGAGGG+AGG | 0.690356 | 3.4:-65810397 | MS.gene05060:CDS |
| GGATCTTAAAAGGAGCCAGG+AGG | 0.690908 | 3.4:-65800771 | MS.gene05060:CDS |
| GTTCGACAACAAGTACATAG+TGG | 0.695714 | 3.4:-65800016 | MS.gene05060:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 65798225 | 65810539 | 65798225 | ID=MS.gene05060 |
| chr3.4 | mRNA | 65798225 | 65810539 | 65798225 | ID=MS.gene05060.t1;Parent=MS.gene05060 |
| chr3.4 | exon | 65810534 | 65810539 | 65810534 | ID=MS.gene05060.t1.exon1;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65810534 | 65810539 | 65810534 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| chr3.4 | exon | 65810377 | 65810482 | 65810377 | ID=MS.gene05060.t1.exon2;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65810377 | 65810482 | 65810377 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| chr3.4 | exon | 65808851 | 65808906 | 65808851 | ID=MS.gene05060.t1.exon3;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65808851 | 65808906 | 65808851 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| chr3.4 | exon | 65800631 | 65800795 | 65800631 | ID=MS.gene05060.t1.exon4;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65800631 | 65800795 | 65800631 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| chr3.4 | exon | 65800017 | 65800100 | 65800017 | ID=MS.gene05060.t1.exon5;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65800017 | 65800100 | 65800017 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| chr3.4 | exon | 65798435 | 65798551 | 65798435 | ID=MS.gene05060.t1.exon6;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65798435 | 65798551 | 65798435 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| chr3.4 | exon | 65798225 | 65798329 | 65798225 | ID=MS.gene05060.t1.exon7;Parent=MS.gene05060.t1 |
| chr3.4 | CDS | 65798225 | 65798329 | 65798225 | ID=cds.MS.gene05060.t1;Parent=MS.gene05060.t1 |
| Gene Sequence |
| Protein sequence |
