AlfalfaGEDB | MS.gene050683

M. sativa cultivar XinJiangDaYe / MS.gene050683

KEGG: K02942
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006414 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044249 GO:0044260 GO:0071704 GO:0110165 GO:1901576
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050683.t1	XP_024625385.1	98.3	59	1	0	1	59	1	59	9.10E-23	116.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050683.t1	Q8LCW9	62.1	58	22	0	2	59	3	60	1.0e-14	80.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050683.t1	G7L532	98.3	59	1	0	1	59	1	59	6.5e-23	116.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050683	MS.gene58497	0.87867	2.33E-69	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050683.t1	MTR_7g118060	93.913	115	2	2	1	112	1	113	2.05e-67	198
MS.gene050683.t1	MTR_8g024350	58.559	111	43	1	1	111	1	108	8.10e-33	110
MS.gene050683.t1	MTR_3g092600	54.054	111	50	1	1	111	1	110	3.48e-22	84.0
MS.gene050683.t1	MTR_2g066750	54.054	111	50	1	1	111	1	110	3.48e-22	84.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050683.t1	AT5G24510	52.542	118	42	4	1	111	1	111	6.50e-31	106
MS.gene050683.t1	AT1G01100	54.545	110	50	0	2	111	3	112	8.19e-28	98.6
MS.gene050683.t1	AT1G01100	54.545	110	50	0	2	111	3	112	8.19e-28	98.6
MS.gene050683.t1	AT1G01100	54.545	110	50	0	2	111	3	112	8.19e-28	98.6
MS.gene050683.t1	AT5G47700	54.955	111	49	1	2	111	3	113	2.06e-26	95.1
MS.gene050683.t1	AT5G47700	54.955	111	49	1	2	111	3	113	2.06e-26	95.1
MS.gene050683.t1	AT4G00810	54.464	112	48	2	2	111	3	113	2.27e-26	94.7
MS.gene050683.t1	AT4G00810	54.464	112	48	2	2	111	3	113	2.27e-26	94.7
MS.gene050683.t1	AT1G01100	47.273	110	42	1	2	111	3	96	3.07e-26	94.0

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCTCTTCTTCCTCTGGCTC+TGG	0.293967	7.3:-551521	None:intergenic
AGCAGCAGCTGGAGCAGCAG+CGG	0.297502	7.3:-551483	None:intergenic
AGAAGAGAGTGACGAAGAAA+TGG	0.327092	7.3:+551535	MS.gene050683:CDS
ATTCGGCTTGTTCGATGAAT+AGG	0.347729	7.3:+551559	MS.gene050683:CDS
AGGTTGATGTTGAATCCTTC+TGG	0.358591	7.3:+551345	MS.gene050683:CDS
CCACCACCTGATGCTGCAAC+TGG	0.368389	7.3:-551458	None:intergenic
GCTGCTCCAGTTGCAGCATC+AGG	0.384530	7.3:+551452	MS.gene050683:CDS
TGCTCCACCTCCAGCAGCAC+TGG	0.392502	7.3:-551420	None:intergenic
TCGTCACTCTCTTCTTCCTC+TGG	0.392693	7.3:-551527	None:intergenic
GATTTGATTGCCAGTGCTGC+TGG	0.422177	7.3:+551410	MS.gene050683:CDS
CAACAAAGTTGTGATGTTGT+CGG	0.447259	7.3:-551312	None:intergenic
ATTGCCAGTGCTGCTGGAGG+TGG	0.452006	7.3:+551416	MS.gene050683:CDS
GAAGAGAGTGACGAAGAAAT+GGG	0.472727	7.3:+551536	MS.gene050683:CDS
GTGATGTTGTCGGCAGTTGC+TGG	0.481555	7.3:-551302	None:intergenic
CCAGTTGCAGCATCAGGTGG+TGG	0.493184	7.3:+551458	MS.gene050683:CDS
GGATTCAACATCAACCTTTG+CGG	0.496398	7.3:-551339	None:intergenic
CTGGAATGTTGTCATCTTCG+AGG	0.500813	7.3:-551283	None:intergenic
AGTGACGAAGAAATGGGATT+CGG	0.508982	7.3:+551542	MS.gene050683:CDS
GCAAGTTTAGCGAATAAAGC+GGG	0.558481	7.3:-551368	None:intergenic
TGTCATCTTCGAGGATGAGA+AGG	0.559827	7.3:-551274	None:intergenic
GCTCCAGTTGCAGCATCAGG+TGG	0.561855	7.3:+551455	MS.gene050683:CDS
AACTTTGTTGAAATCCGCAA+AGG	0.566474	7.3:+551325	MS.gene050683:CDS
AGCGAATAAAGCGGGCCAGA+AGG	0.567952	7.3:-551360	None:intergenic
AGCAAGTTTAGCGAATAAAG+CGG	0.583424	7.3:-551369	None:intergenic
TGGAGGTGGAGCAGCTGTTG+CGG	0.612651	7.3:+551430	MS.gene050683:CDS
GTCATCTTCGAGGATGAGAA+GGG	0.616441	7.3:-551273	None:intergenic
TGAGAAGAAACCAGAGCCAG+AGG	0.638704	7.3:+551511	MS.gene050683:CDS
TTGATTGCCAGTGCTGCTGG+AGG	0.642263	7.3:+551413	MS.gene050683:CDS
TGTAGTGTGAAAATGTCGCT+CGG	0.684963	7.3:+551230	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGCTGAGAAGAAAAATATTA+GGG	+	chr7.3:551388-551407	MS.gene050683:CDS	25.0%
!	TTGCTGAGAAGAAAAATATT+AGG	+	chr7.3:551387-551406	MS.gene050683:CDS	25.0%
	AACTTTGTTGAAATCCGCAA+AGG	+	chr7.3:551325-551344	MS.gene050683:CDS	35.0%
	AGCAAGTTTAGCGAATAAAG+CGG	-	chr7.3:551372-551391	None:intergenic	35.0%
	CAACAAAGTTGTGATGTTGT+CGG	-	chr7.3:551315-551334	None:intergenic	35.0%
	AGAAGAGAGTGACGAAGAAA+TGG	+	chr7.3:551535-551554	MS.gene050683:CDS	40.0%
	AGTGACGAAGAAATGGGATT+CGG	+	chr7.3:551542-551561	MS.gene050683:CDS	40.0%
	GAAGAGAGTGACGAAGAAAT+GGG	+	chr7.3:551536-551555	MS.gene050683:CDS	40.0%
	GCAAGTTTAGCGAATAAAGC+GGG	-	chr7.3:551371-551390	None:intergenic	40.0%
	GGATTCAACATCAACCTTTG+CGG	-	chr7.3:551342-551361	None:intergenic	40.0%
!!	AGGTTGATGTTGAATCCTTC+TGG	+	chr7.3:551345-551364	MS.gene050683:CDS	40.0%
	CTGGAATGTTGTCATCTTCG+AGG	-	chr7.3:551286-551305	None:intergenic	45.0%
	GTCATCTTCGAGGATGAGAA+GGG	-	chr7.3:551276-551295	None:intergenic	45.0%
	TGTCATCTTCGAGGATGAGA+AGG	-	chr7.3:551277-551296	None:intergenic	45.0%
	GATTTGATTGCCAGTGCTGC+TGG	+	chr7.3:551410-551429	MS.gene050683:CDS	50.0%
	TCGTCACTCTCTTCTTCCTC+TGG	-	chr7.3:551530-551549	None:intergenic	50.0%
	TGAGAAGAAACCAGAGCCAG+AGG	+	chr7.3:551511-551530	MS.gene050683:CDS	50.0%
	AGCGAATAAAGCGGGCCAGA+AGG	-	chr7.3:551363-551382	None:intergenic	55.0%
	GTGATGTTGTCGGCAGTTGC+TGG	-	chr7.3:551305-551324	None:intergenic	55.0%
!	CTCTCTTCTTCCTCTGGCTC+TGG	-	chr7.3:551524-551543	None:intergenic	55.0%
!	TTGATTGCCAGTGCTGCTGG+AGG	+	chr7.3:551413-551432	MS.gene050683:CDS	55.0%
	CCACCACCTGATGCTGCAAC+TGG	-	chr7.3:551461-551480	None:intergenic	60.0%
	CCAGTTGCAGCATCAGGTGG+TGG	+	chr7.3:551458-551477	MS.gene050683:CDS	60.0%
	GCTCCAGTTGCAGCATCAGG+TGG	+	chr7.3:551455-551474	MS.gene050683:CDS	60.0%
	GCTGCTCCAGTTGCAGCATC+AGG	+	chr7.3:551452-551471	MS.gene050683:CDS	60.0%
	TGGAGGTGGAGCAGCTGTTG+CGG	+	chr7.3:551430-551449	MS.gene050683:CDS	60.0%
	TTCTCAGCAGCAGCAGCAGC+TGG	-	chr7.3:551497-551516	None:intergenic	60.0%
!	ATTGCCAGTGCTGCTGGAGG+TGG	+	chr7.3:551416-551435	MS.gene050683:CDS	60.0%
	AGCAGCAGCTGGAGCAGCAG+CGG	-	chr7.3:551486-551505	None:intergenic	65.0%
	TGCTCCACCTCCAGCAGCAC+TGG	-	chr7.3:551423-551442	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	551242	551580	551242	ID=MS.gene050683
chr7.3	mRNA	551242	551580	551242	ID=MS.gene050683.t1;Parent=MS.gene050683
chr7.3	exon	551242	551580	551242	ID=MS.gene050683.t1.exon1;Parent=MS.gene050683.t1
chr7.3	CDS	551242	551580	551242	ID=cds.MS.gene050683.t1;Parent=MS.gene050683.t1

Gene Sequence	>MS.gene050683 ATGTCGCTCGGAGAAACTGCTTGCTCATACGCCCTTCTCATCCTCGAAGATGACAACATTCCAGCAACTGCCGACAACATCACAACTTTGTTGAAATCCGCAAAGGTTGATGTTGAATCCTTCTGGCCCGCTTTATTCGCTAAACTTGCTGAGAAGAAAAATATTAGGGATTTGATTGCCAGTGCTGCTGGAGGTGGAGCAGCTGTTGCGGCTGCTCCAGTTGCAGCATCAGGTGGTGGTGCCGCTGCTGCTCCAGCTGCTGCTGCTGCTGAGAAGAAACCAGAGCCAGAGGAAGAAGAGAGTGACGAAGAAATGGGATTCGGCTTGTTCGATGAATAG
Protein sequence	>MS.gene050683.t1 MSLGETACSYALLILEDDNIPATADNITTLLKSAKVDVESFWPALFAKLAEKKNIRDLIASAAGGGAAVAAAPVAASGGGAAAAPAAAAAEKKPEPEEEESDEEMGFGLFDE