AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58497


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58497.t1 MTR_7g118060 93.913 115 2 2 1 112 1 113 2.05e-67 198
MS.gene58497.t1 MTR_8g024350 58.559 111 43 1 1 111 1 108 8.10e-33 110
MS.gene58497.t1 MTR_3g092600 54.054 111 50 1 1 111 1 110 3.48e-22 84.0
MS.gene58497.t1 MTR_2g066750 54.054 111 50 1 1 111 1 110 3.48e-22 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58497.t1 AT5G24510 52.542 118 42 4 1 111 1 111 6.50e-31 106
MS.gene58497.t1 AT1G01100 54.545 110 50 0 2 111 3 112 8.19e-28 98.6
MS.gene58497.t1 AT1G01100 54.545 110 50 0 2 111 3 112 8.19e-28 98.6
MS.gene58497.t1 AT1G01100 54.545 110 50 0 2 111 3 112 8.19e-28 98.6
MS.gene58497.t1 AT5G47700 54.955 111 49 1 2 111 3 113 2.06e-26 95.1
MS.gene58497.t1 AT5G47700 54.955 111 49 1 2 111 3 113 2.06e-26 95.1
MS.gene58497.t1 AT4G00810 54.464 112 48 2 2 111 3 113 2.27e-26 94.7
MS.gene58497.t1 AT4G00810 54.464 112 48 2 2 111 3 113 2.27e-26 94.7
MS.gene58497.t1 AT1G01100 47.273 110 42 1 2 111 3 96 3.07e-26 94.0

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTCTTCCTCTGGCTC+TGG 0.293967 7.4:-540757 None:intergenic
AGCAGCAGCTGGAGCAGCAG+CGG 0.297502 7.4:-540719 None:intergenic
AGAAGAGAGTGACGAAGAAA+TGG 0.327092 7.4:+540771 MS.gene58497:CDS
ATTCGGCTTGTTCGATGAAT+AGG 0.347729 7.4:+540795 MS.gene58497:CDS
AGGTTGATGTTGAATCCTTC+TGG 0.358591 7.4:+540581 MS.gene58497:CDS
CCACCACCTGATGCTGCAAC+TGG 0.368389 7.4:-540694 None:intergenic
GCTGCTCCAGTTGCAGCATC+AGG 0.384530 7.4:+540688 MS.gene58497:CDS
TGCTCCACCTCCAGCAGCAC+TGG 0.392502 7.4:-540656 None:intergenic
TCGTCACTCTCTTCTTCCTC+TGG 0.392693 7.4:-540763 None:intergenic
GATTTGATTGCCAGTGCTGC+TGG 0.422177 7.4:+540646 MS.gene58497:CDS
CAACAAAGTTGTGATGTTGT+CGG 0.447259 7.4:-540548 None:intergenic
ATTGCCAGTGCTGCTGGAGG+TGG 0.452006 7.4:+540652 MS.gene58497:CDS
GAAGAGAGTGACGAAGAAAT+GGG 0.472727 7.4:+540772 MS.gene58497:CDS
GTGATGTTGTCGGCAGTTGC+TGG 0.481555 7.4:-540538 None:intergenic
CCAGTTGCAGCATCAGGTGG+TGG 0.493184 7.4:+540694 MS.gene58497:CDS
GGATTCAACATCAACCTTTG+CGG 0.496398 7.4:-540575 None:intergenic
CTGGAATGTTGTCATCTTCG+AGG 0.500813 7.4:-540519 None:intergenic
AGTGACGAAGAAATGGGATT+CGG 0.508982 7.4:+540778 MS.gene58497:CDS
GCAAGTTTAGCGAATAAAGC+GGG 0.558481 7.4:-540604 None:intergenic
TGTCATCTTCGAGGATGAGA+AGG 0.559827 7.4:-540510 None:intergenic
GCTCCAGTTGCAGCATCAGG+TGG 0.561855 7.4:+540691 MS.gene58497:CDS
AACTTTGTTGAAATCCGCAA+AGG 0.566474 7.4:+540561 MS.gene58497:CDS
AGCGAATAAAGCGGGCCAGA+AGG 0.567952 7.4:-540596 None:intergenic
AGCAAGTTTAGCGAATAAAG+CGG 0.583424 7.4:-540605 None:intergenic
TGGAGGTGGAGCAGCTGTTG+CGG 0.612651 7.4:+540666 MS.gene58497:CDS
GTCATCTTCGAGGATGAGAA+GGG 0.616441 7.4:-540509 None:intergenic
TGAGAAGAAACCAGAGCCAG+AGG 0.638704 7.4:+540747 MS.gene58497:CDS
TTGATTGCCAGTGCTGCTGG+AGG 0.642263 7.4:+540649 MS.gene58497:CDS
TGTAGTGTGAAAATGTCGCT+CGG 0.684963 7.4:+540466 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGCTGAGAAGAAAAATATTA+GGG + chr7.4:540624-540643 MS.gene58497:CDS 25.0%
! TTGCTGAGAAGAAAAATATT+AGG + chr7.4:540623-540642 MS.gene58497:CDS 25.0%
AACTTTGTTGAAATCCGCAA+AGG + chr7.4:540561-540580 MS.gene58497:CDS 35.0%
AGCAAGTTTAGCGAATAAAG+CGG - chr7.4:540608-540627 None:intergenic 35.0%
CAACAAAGTTGTGATGTTGT+CGG - chr7.4:540551-540570 None:intergenic 35.0%
AGAAGAGAGTGACGAAGAAA+TGG + chr7.4:540771-540790 MS.gene58497:CDS 40.0%
AGTGACGAAGAAATGGGATT+CGG + chr7.4:540778-540797 MS.gene58497:CDS 40.0%
GAAGAGAGTGACGAAGAAAT+GGG + chr7.4:540772-540791 MS.gene58497:CDS 40.0%
GCAAGTTTAGCGAATAAAGC+GGG - chr7.4:540607-540626 None:intergenic 40.0%
GGATTCAACATCAACCTTTG+CGG - chr7.4:540578-540597 None:intergenic 40.0%
!! AGGTTGATGTTGAATCCTTC+TGG + chr7.4:540581-540600 MS.gene58497:CDS 40.0%
CTGGAATGTTGTCATCTTCG+AGG - chr7.4:540522-540541 None:intergenic 45.0%
GTCATCTTCGAGGATGAGAA+GGG - chr7.4:540512-540531 None:intergenic 45.0%
TGTCATCTTCGAGGATGAGA+AGG - chr7.4:540513-540532 None:intergenic 45.0%
GATTTGATTGCCAGTGCTGC+TGG + chr7.4:540646-540665 MS.gene58497:CDS 50.0%
TCGTCACTCTCTTCTTCCTC+TGG - chr7.4:540766-540785 None:intergenic 50.0%
TGAGAAGAAACCAGAGCCAG+AGG + chr7.4:540747-540766 MS.gene58497:CDS 50.0%
AGCGAATAAAGCGGGCCAGA+AGG - chr7.4:540599-540618 None:intergenic 55.0%
GTGATGTTGTCGGCAGTTGC+TGG - chr7.4:540541-540560 None:intergenic 55.0%
! CTCTCTTCTTCCTCTGGCTC+TGG - chr7.4:540760-540779 None:intergenic 55.0%
! TTGATTGCCAGTGCTGCTGG+AGG + chr7.4:540649-540668 MS.gene58497:CDS 55.0%
CCACCACCTGATGCTGCAAC+TGG - chr7.4:540697-540716 None:intergenic 60.0%
CCAGTTGCAGCATCAGGTGG+TGG + chr7.4:540694-540713 MS.gene58497:CDS 60.0%
GCTCCAGTTGCAGCATCAGG+TGG + chr7.4:540691-540710 MS.gene58497:CDS 60.0%
GCTGCTCCAGTTGCAGCATC+AGG + chr7.4:540688-540707 MS.gene58497:CDS 60.0%
TGGAGGTGGAGCAGCTGTTG+CGG + chr7.4:540666-540685 MS.gene58497:CDS 60.0%
TTCTCAGCAGCAGCAGCAGC+TGG - chr7.4:540733-540752 None:intergenic 60.0%
! ATTGCCAGTGCTGCTGGAGG+TGG + chr7.4:540652-540671 MS.gene58497:CDS 60.0%
AGCAGCAGCTGGAGCAGCAG+CGG - chr7.4:540722-540741 None:intergenic 65.0%
TGCTCCACCTCCAGCAGCAC+TGG - chr7.4:540659-540678 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 540478 540816 540478 ID=MS.gene58497
chr7.4 mRNA 540478 540816 540478 ID=MS.gene58497.t1;Parent=MS.gene58497
chr7.4 exon 540478 540816 540478 ID=MS.gene58497.t1.exon1;Parent=MS.gene58497.t1
chr7.4 CDS 540478 540816 540478 ID=cds.MS.gene58497.t1;Parent=MS.gene58497.t1
Gene Sequence

>MS.gene58497

ATGTCGCTCGGAGAAACTGCTTGCTCATACGCCCTTCTCATCCTCGAAGATGACAACATTCCAGCAACTGCCGACAACATCACAACTTTGTTGAAATCCGCAAAGGTTGATGTTGAATCCTTCTGGCCCGCTTTATTCGCTAAACTTGCTGAGAAGAAAAATATTAGGGATTTGATTGCCAGTGCTGCTGGAGGTGGAGCAGCTGTTGCGGCTGCTCCAGTTGCAGCATCAGGTGGTGGTGCCGCTGCTGCTCCAGCTGCTGCTGCTGCTGAGAAGAAACCAGAGCCAGAGGAAGAAGAGAGTGACGAAGAAATGGGATTCGGCTTGTTCGATGAATAG

Protein sequence

>MS.gene58497.t1

MSLGETACSYALLILEDDNIPATADNITTLLKSAKVDVESFWPALFAKLAEKKNIRDLIASAAGGGAAVAAAPVAASGGGAAAAPAAAAAEKKPEPEEEESDEEMGFGLFDE