Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050684.t1 | XP_024625385.1 | 67.9 | 53 | 17 | 0 | 1 | 53 | 1 | 53 | 8.40E-09 | 68.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050684.t1 | G7L532 | 67.9 | 53 | 17 | 0 | 1 | 53 | 1 | 53 | 6.1e-09 | 68.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050684 | MS.gene58497 | 0.911872 | 3.72E-83 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050684.t1 | MTR_7g118060 | 67.925 | 53 | 17 | 0 | 1 | 53 | 1 | 53 | 2.35e-18 | 72.0 |
| MS.gene050684.t1 | MTR_8g024350 | 47.170 | 53 | 28 | 0 | 1 | 53 | 1 | 53 | 7.53e-11 | 52.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTTGATGTTGAATCCTTC+TGG | 0.363218 | 7.4:+528995 | MS.gene050684:CDS |
| CAACAAAGTTGTGATGTTGT+CGG | 0.447259 | 7.4:-528963 | None:intergenic |
| GTGATGTTGTCGGCAGTTGC+TGG | 0.481555 | 7.4:-528953 | None:intergenic |
| CTGGAATGTTGTCATCTTCG+AGG | 0.500813 | 7.4:-528934 | None:intergenic |
| GCAAGTTTAGCGAATAAAGC+GGG | 0.558481 | 7.4:-529018 | None:intergenic |
| TGTCATCTTCGAGGATGAGA+AGG | 0.559827 | 7.4:-528925 | None:intergenic |
| AGCGAATAAAGCGGGCCAGA+AGG | 0.567952 | 7.4:-529010 | None:intergenic |
| AGCAAGTTTAGCGAATAAAG+CGG | 0.583424 | 7.4:-529019 | None:intergenic |
| CAACTTTGTTGAAATCCGCA+AGG | 0.612147 | 7.4:+528975 | MS.gene050684:CDS |
| GTCATCTTCGAGGATGAGAA+GGG | 0.616441 | 7.4:-528924 | None:intergenic |
| AGGATTCAACATCAACCTTG+CGG | 0.660755 | 7.4:-528990 | None:intergenic |
| TGTAGTGTGAAAATGTCGCT+CGG | 0.684963 | 7.4:+528881 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGCTGAGAAGAAAAATATTA+GGG | + | chr7.4:529038-529057 | MS.gene050684:CDS | 25.0% |
| ! | TTGCTGAGAAGAAAAATATT+AGG | + | chr7.4:529037-529056 | MS.gene050684:CDS | 25.0% |
| AGCAAGTTTAGCGAATAAAG+CGG | - | chr7.4:529022-529041 | None:intergenic | 35.0% | |
| CAACAAAGTTGTGATGTTGT+CGG | - | chr7.4:528966-528985 | None:intergenic | 35.0% | |
| AGGATTCAACATCAACCTTG+CGG | - | chr7.4:528993-529012 | None:intergenic | 40.0% | |
| CAACTTTGTTGAAATCCGCA+AGG | + | chr7.4:528975-528994 | MS.gene050684:CDS | 40.0% | |
| GCAAGTTTAGCGAATAAAGC+GGG | - | chr7.4:529021-529040 | None:intergenic | 40.0% | |
| !! | AGGTTGATGTTGAATCCTTC+TGG | + | chr7.4:528995-529014 | MS.gene050684:CDS | 40.0% |
| CTGGAATGTTGTCATCTTCG+AGG | - | chr7.4:528937-528956 | None:intergenic | 45.0% | |
| GTCATCTTCGAGGATGAGAA+GGG | - | chr7.4:528927-528946 | None:intergenic | 45.0% | |
| TGTCATCTTCGAGGATGAGA+AGG | - | chr7.4:528928-528947 | None:intergenic | 45.0% | |
| AGCGAATAAAGCGGGCCAGA+AGG | - | chr7.4:529013-529032 | None:intergenic | 55.0% | |
| GTGATGTTGTCGGCAGTTGC+TGG | - | chr7.4:528956-528975 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 528893 | 529066 | 528893 | ID=MS.gene050684 |
| chr7.4 | mRNA | 528893 | 529066 | 528893 | ID=MS.gene050684.t1;Parent=MS.gene050684 |
| chr7.4 | exon | 528893 | 529066 | 528893 | ID=MS.gene050684.t1.exon1;Parent=MS.gene050684.t1 |
| chr7.4 | CDS | 528893 | 529066 | 528893 | ID=cds.MS.gene050684.t1;Parent=MS.gene050684.t1 |
| Gene Sequence |
| Protein sequence |
