Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050938.t1 | RHN49201.1 | 96.3 | 215 | 8 | 0 | 1 | 215 | 1 | 215 | 1.80E-120 | 441.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050938.t1 | Q9LZM4 | 37.9 | 190 | 109 | 5 | 32 | 214 | 43 | 230 | 2.1e-32 | 140.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050938.t1 | A0A396H9I2 | 96.3 | 215 | 8 | 0 | 1 | 215 | 1 | 215 | 1.3e-120 | 441.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050938 | MS.gene054421 | 0.830546 | 2.76E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050938.t1 | MTR_7g112440 | 97.073 | 205 | 6 | 0 | 11 | 215 | 1 | 205 | 6.62e-147 | 407 |
| MS.gene050938.t1 | MTR_3g069050 | 36.649 | 191 | 117 | 3 | 26 | 214 | 22 | 210 | 2.06e-38 | 140 |
| MS.gene050938.t1 | MTR_7g103180 | 37.306 | 193 | 111 | 5 | 32 | 214 | 33 | 225 | 8.26e-38 | 139 |
| MS.gene050938.t1 | MTR_5g037310 | 27.193 | 228 | 131 | 10 | 8 | 212 | 7 | 222 | 6.04e-12 | 63.9 |
| MS.gene050938.t1 | MTR_5g037310 | 27.511 | 229 | 129 | 11 | 8 | 212 | 7 | 222 | 6.51e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050938.t1 | AT5G02070 | 37.056 | 197 | 115 | 5 | 25 | 214 | 36 | 230 | 2.16e-36 | 135 |
| MS.gene050938.t1 | AT3G53840 | 36.036 | 222 | 129 | 7 | 3 | 215 | 4 | 221 | 7.70e-34 | 127 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGTGTGTTTAAGTATAT+TGG | 0.180053 | 7.1:-3646615 | None:intergenic |
| CAACCCTAAGTCTGATCTTA+TGG | 0.212153 | 7.1:-3646868 | None:intergenic |
| TGCATGCAATTTAGTTAATT+AGG | 0.230899 | 7.1:-3646983 | None:intergenic |
| TTAGAGTTCTTGTCAGCAAC+AGG | 0.338196 | 7.1:+3646533 | MS.gene050938:CDS |
| TGTTATTCTTCTGATCTCAT+TGG | 0.347587 | 7.1:+3646635 | MS.gene050938:CDS |
| CAAGTGTGTTTAAGTATATT+GGG | 0.351271 | 7.1:-3646614 | None:intergenic |
| CAGCATTTGTAGACAGTTTG+AGG | 0.366269 | 7.1:+3646772 | MS.gene050938:CDS |
| CTGTTTGAGGAACAGTTTAG+AGG | 0.382089 | 7.1:-3646752 | None:intergenic |
| AACCCTAAGTCTGATCTTAT+GGG | 0.401892 | 7.1:-3646867 | None:intergenic |
| TACTGCTTACACTTGTTTGG+TGG | 0.409415 | 7.1:+3646898 | MS.gene050938:CDS |
| TTGTACTGCTTACACTTGTT+TGG | 0.410029 | 7.1:+3646895 | MS.gene050938:CDS |
| AGAAAGATTCCATGTATCCT+TGG | 0.438666 | 7.1:+3646333 | None:intergenic |
| TGATTAGTAGAAAGAGTAGA+AGG | 0.443383 | 7.1:-3646382 | None:intergenic |
| ATTCCATGTATCCTTGGGAT+TGG | 0.449562 | 7.1:+3646339 | None:intergenic |
| TATTCTTCTGATCTCATTGG+TGG | 0.451225 | 7.1:+3646638 | MS.gene050938:CDS |
| TTCCCATAAGATCAGACTTA+GGG | 0.466503 | 7.1:+3646865 | MS.gene050938:CDS |
| GAAAGATTCCATGTATCCTT+GGG | 0.467489 | 7.1:+3646334 | None:intergenic |
| TCTTCTGATCTCATTGGTGG+AGG | 0.471616 | 7.1:+3646641 | MS.gene050938:CDS |
| TTTCTACTAATCACTGGTCT+TGG | 0.477113 | 7.1:+3646392 | MS.gene050938:CDS |
| AACAGTTTAGAGGAGATTGA+AGG | 0.483257 | 7.1:-3646742 | None:intergenic |
| ATTATAAGCTTGTTAGCACT+TGG | 0.485174 | 7.1:-3646584 | None:intergenic |
| CTACTCTTTCTACTAATCAC+TGG | 0.492211 | 7.1:+3646386 | MS.gene050938:CDS |
| ATTCCAATCCCAAGGATACA+TGG | 0.495507 | 7.1:-3646342 | None:intergenic |
| GTTGAGTGCAGAAATATTGA+AGG | 0.498744 | 7.1:-3646687 | None:intergenic |
| GGATTGGAATAGTTTAAAGA+TGG | 0.503276 | 7.1:+3646355 | MS.gene050938:CDS |
| CTGTCTACAAATGCTGTTTG+AGG | 0.506654 | 7.1:-3646765 | None:intergenic |
| ATAAGATCAGACTTAGGGTT+GGG | 0.510350 | 7.1:+3646870 | MS.gene050938:CDS |
| ATTCCCATAAGATCAGACTT+AGG | 0.515395 | 7.1:+3646864 | MS.gene050938:CDS |
| ATGATGATTGTGGTGACAAA+AGG | 0.535496 | 7.1:+3646483 | MS.gene050938:CDS |
| ATGCAATTTAGTTAATTAGG+AGG | 0.544113 | 7.1:-3646980 | None:intergenic |
| CAATCATCATTAGTGCTAAG+TGG | 0.546790 | 7.1:-3646470 | None:intergenic |
| TCATTAGTGCTAAGTGGGTG+AGG | 0.560513 | 7.1:-3646464 | None:intergenic |
| TTATAAGCTTGTTAGCACTT+GGG | 0.573998 | 7.1:-3646583 | None:intergenic |
| TTTAAACTATTCCAATCCCA+AGG | 0.579737 | 7.1:-3646350 | None:intergenic |
| CATAAGATCAGACTTAGGGT+TGG | 0.583330 | 7.1:+3646869 | MS.gene050938:CDS |
| AATCATCATTAGTGCTAAGT+GGG | 0.586032 | 7.1:-3646469 | None:intergenic |
| CAAGGAGATGCATTGATCAC+GGG | 0.586885 | 7.1:-3646416 | None:intergenic |
| GCATCTCCTTGTTCAAATTG+TGG | 0.598062 | 7.1:+3646428 | MS.gene050938:CDS |
| ACAAGGAGATGCATTGATCA+CGG | 0.611816 | 7.1:-3646417 | None:intergenic |
| ACTATGGGATTGAGTTGCAA+TGG | 0.615433 | 7.1:+3646954 | MS.gene050938:CDS |
| TTGAGTGCAGAAATATTGAA+GGG | 0.632612 | 7.1:-3646686 | None:intergenic |
| AAGTCACCACAATTTGAACA+AGG | 0.634351 | 7.1:-3646434 | None:intergenic |
| TAGAGTTCTTGTCAGCAACA+GGG | 0.637502 | 7.1:+3646534 | MS.gene050938:CDS |
| GTGTGTTTAAGTATATTGGG+TGG | 0.649267 | 7.1:-3646611 | None:intergenic |
| CTTAGCACTAATGATGATTG+TGG | 0.667956 | 7.1:+3646473 | MS.gene050938:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTAAAAAATGGAACTAT+GGG | + | chr7.1:3646939-3646958 | MS.gene050938:CDS | 20.0% |
| !!! | TTTTAAGTAATGACAACAAA+GGG | - | chr7.1:3646831-3646850 | None:intergenic | 20.0% |
| ! | ACAAGTGTGTTTAAGTATAT+TGG | - | chr7.1:3646618-3646637 | None:intergenic | 25.0% |
| ! | CAAGTGTGTTTAAGTATATT+GGG | - | chr7.1:3646617-3646636 | None:intergenic | 25.0% |
| !!! | CATTTTTTAAAAGGTTCATC+AGG | - | chr7.1:3646932-3646951 | None:intergenic | 25.0% |
| !!! | CCATAGTTCCATTTTTTAAA+AGG | - | chr7.1:3646941-3646960 | None:intergenic | 25.0% |
| !!! | CCTTTTAAAAAATGGAACTA+TGG | + | chr7.1:3646938-3646957 | MS.gene050938:CDS | 25.0% |
| !!! | CTGATGAACCTTTTAAAAAA+TGG | + | chr7.1:3646930-3646949 | MS.gene050938:CDS | 25.0% |
| !!! | CTTTTAAGTAATGACAACAA+AGG | - | chr7.1:3646832-3646851 | None:intergenic | 25.0% |
| AATCATCATTAGTGCTAAGT+GGG | - | chr7.1:3646472-3646491 | None:intergenic | 30.0% | |
| GGATTGGAATAGTTTAAAGA+TGG | + | chr7.1:3646355-3646374 | MS.gene050938:CDS | 30.0% | |
| TGATTAGTAGAAAGAGTAGA+AGG | - | chr7.1:3646385-3646404 | None:intergenic | 30.0% | |
| TGTTATTCTTCTGATCTCAT+TGG | + | chr7.1:3646635-3646654 | MS.gene050938:CDS | 30.0% | |
| TTGAGTGCAGAAATATTGAA+GGG | - | chr7.1:3646689-3646708 | None:intergenic | 30.0% | |
| TTTAAACTATTCCAATCCCA+AGG | - | chr7.1:3646353-3646372 | None:intergenic | 30.0% | |
| !! | ATTATAAGCTTGTTAGCACT+TGG | - | chr7.1:3646587-3646606 | None:intergenic | 30.0% |
| !! | TTATAAGCTTGTTAGCACTT+GGG | - | chr7.1:3646586-3646605 | None:intergenic | 30.0% |
| AACAGTTTAGAGGAGATTGA+AGG | - | chr7.1:3646745-3646764 | None:intergenic | 35.0% | |
| AACCCTAAGTCTGATCTTAT+GGG | - | chr7.1:3646870-3646889 | None:intergenic | 35.0% | |
| AAGTCACCACAATTTGAACA+AGG | - | chr7.1:3646437-3646456 | None:intergenic | 35.0% | |
| ATAAGATCAGACTTAGGGTT+GGG | + | chr7.1:3646870-3646889 | MS.gene050938:CDS | 35.0% | |
| ATGATGATTGTGGTGACAAA+AGG | + | chr7.1:3646483-3646502 | MS.gene050938:CDS | 35.0% | |
| ATTCCCATAAGATCAGACTT+AGG | + | chr7.1:3646864-3646883 | MS.gene050938:CDS | 35.0% | |
| CAATCATCATTAGTGCTAAG+TGG | - | chr7.1:3646473-3646492 | None:intergenic | 35.0% | |
| CTACTCTTTCTACTAATCAC+TGG | + | chr7.1:3646386-3646405 | MS.gene050938:CDS | 35.0% | |
| CTTAGCACTAATGATGATTG+TGG | + | chr7.1:3646473-3646492 | MS.gene050938:CDS | 35.0% | |
| GTGTGTTTAAGTATATTGGG+TGG | - | chr7.1:3646614-3646633 | None:intergenic | 35.0% | |
| GTTGAGTGCAGAAATATTGA+AGG | - | chr7.1:3646690-3646709 | None:intergenic | 35.0% | |
| TAGACAGTTTGAGGAAAAAG+TGG | + | chr7.1:3646781-3646800 | MS.gene050938:CDS | 35.0% | |
| TATTCTTCTGATCTCATTGG+TGG | + | chr7.1:3646638-3646657 | MS.gene050938:CDS | 35.0% | |
| TTCCCATAAGATCAGACTTA+GGG | + | chr7.1:3646865-3646884 | MS.gene050938:CDS | 35.0% | |
| TTGTACTGCTTACACTTGTT+TGG | + | chr7.1:3646895-3646914 | MS.gene050938:CDS | 35.0% | |
| ! | TAGCACTTGGGTCAATTTTA+AGG | - | chr7.1:3646574-3646593 | None:intergenic | 35.0% |
| ! | TTTCTACTAATCACTGGTCT+TGG | + | chr7.1:3646392-3646411 | MS.gene050938:CDS | 35.0% |
| AAAAAGTGGAGGAGGGAAAT+GGG | + | chr7.1:3646795-3646814 | MS.gene050938:CDS | 40.0% | |
| ACAGTTTGAGGAAAAAGTGG+AGG | + | chr7.1:3646784-3646803 | MS.gene050938:CDS | 40.0% | |
| CAACCCTAAGTCTGATCTTA+TGG | - | chr7.1:3646871-3646890 | None:intergenic | 40.0% | |
| CAGCATTTGTAGACAGTTTG+AGG | + | chr7.1:3646772-3646791 | MS.gene050938:CDS | 40.0% | |
| CATAAGATCAGACTTAGGGT+TGG | + | chr7.1:3646869-3646888 | MS.gene050938:CDS | 40.0% | |
| CTGTTTGAGGAACAGTTTAG+AGG | - | chr7.1:3646755-3646774 | None:intergenic | 40.0% | |
| GCATCTCCTTGTTCAAATTG+TGG | + | chr7.1:3646428-3646447 | MS.gene050938:CDS | 40.0% | |
| TACTGCTTACACTTGTTTGG+TGG | + | chr7.1:3646898-3646917 | MS.gene050938:CDS | 40.0% | |
| TAGAGTTCTTGTCAGCAACA+GGG | + | chr7.1:3646534-3646553 | MS.gene050938:CDS | 40.0% | |
| TTAGAGTTCTTGTCAGCAAC+AGG | + | chr7.1:3646533-3646552 | MS.gene050938:CDS | 40.0% | |
| TTTGAGGAAAAAGTGGAGGA+GGG | + | chr7.1:3646788-3646807 | MS.gene050938:CDS | 40.0% | |
| ! | ACAAGGAGATGCATTGATCA+CGG | - | chr7.1:3646420-3646439 | None:intergenic | 40.0% |
| ! | ACTATGGGATTGAGTTGCAA+TGG | + | chr7.1:3646954-3646973 | MS.gene050938:CDS | 40.0% |
| ! | CTGTCTACAAATGCTGTTTG+AGG | - | chr7.1:3646768-3646787 | None:intergenic | 40.0% |
| GAAAAAGTGGAGGAGGGAAA+TGG | + | chr7.1:3646794-3646813 | MS.gene050938:CDS | 45.0% | |
| GTTTGAGGAAAAAGTGGAGG+AGG | + | chr7.1:3646787-3646806 | MS.gene050938:CDS | 45.0% | |
| TCATTAGTGCTAAGTGGGTG+AGG | - | chr7.1:3646467-3646486 | None:intergenic | 45.0% | |
| TCTTCTGATCTCATTGGTGG+AGG | + | chr7.1:3646641-3646660 | MS.gene050938:CDS | 45.0% | |
| ! | CAAGGAGATGCATTGATCAC+GGG | - | chr7.1:3646419-3646438 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 3646344 | 3646991 | 3646344 | ID=MS.gene050938 |
| chr7.1 | mRNA | 3646344 | 3646991 | 3646344 | ID=MS.gene050938.t1;Parent=MS.gene050938 |
| chr7.1 | exon | 3646344 | 3646991 | 3646344 | ID=MS.gene050938.t1.exon1;Parent=MS.gene050938.t1 |
| chr7.1 | CDS | 3646344 | 3646991 | 3646344 | ID=cds.MS.gene050938.t1;Parent=MS.gene050938.t1 |
| Gene Sequence |
| Protein sequence |
