AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054421


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054421.t1 MTR_7g112440 97.561 205 5 0 11 215 1 205 3.62e-147 408
MS.gene054421.t1 MTR_3g069050 37.173 191 116 3 26 214 22 210 1.44e-38 140
MS.gene054421.t1 MTR_7g103180 37.824 193 110 5 32 214 33 225 6.27e-38 139
MS.gene054421.t1 MTR_5g037310 27.193 228 131 10 8 212 7 222 5.59e-12 63.9
MS.gene054421.t1 MTR_5g037310 27.111 225 135 10 8 212 7 222 6.27e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054421.t1 AT5G02070 37.563 197 114 5 25 214 36 230 1.46e-36 135
MS.gene054421.t1 AT3G53840 36.486 222 128 7 3 215 4 221 5.62e-34 128

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGTGTGTTTAAGTATAT+TGG 0.180053 7.4:-4245247 None:intergenic
CAACCCTAAGTCTGATCTTA+TGG 0.212153 7.4:-4245500 None:intergenic
TGCATGCAATTTAGTTAATT+AGG 0.264632 7.4:-4245615 None:intergenic
TTAGAGTTCTTGTCAGCAAC+AGG 0.338196 7.4:+4245165 MS.gene054421:CDS
TGTTATTCTTCTGATCTCAT+TGG 0.347587 7.4:+4245267 MS.gene054421:CDS
CAAGTGTGTTTAAGTATATT+GGG 0.351271 7.4:-4245246 None:intergenic
CTGTTTGAAGAACAGTTTAG+AGG 0.353341 7.4:-4245384 None:intergenic
CAGCATTTGTAGACAGTTTG+AGG 0.366269 7.4:+4245404 MS.gene054421:CDS
AACCCTAAGTCTGATCTTAT+GGG 0.401892 7.4:-4245499 None:intergenic
TACTGCTTACACTTGTTTGG+TGG 0.409415 7.4:+4245530 MS.gene054421:CDS
TTGTACTGCTTACACTTGTT+TGG 0.410029 7.4:+4245527 MS.gene054421:CDS
AGAAAGATTCCATGTATCCT+TGG 0.438666 7.4:+4244965 None:intergenic
TGATTAGTAGAAAGAGTAGA+AGG 0.443383 7.4:-4245014 None:intergenic
ATTCCATGTATCCTTGGGAT+TGG 0.449562 7.4:+4244971 None:intergenic
TATTCTTCTGATCTCATTGG+TGG 0.451225 7.4:+4245270 MS.gene054421:CDS
TTCCCATAAGATCAGACTTA+GGG 0.466503 7.4:+4245497 MS.gene054421:CDS
GAAAGATTCCATGTATCCTT+GGG 0.467489 7.4:+4244966 None:intergenic
TCTTCTGATCTCATTGGTGG+AGG 0.471616 7.4:+4245273 MS.gene054421:CDS
ATTACAAGCTTGTTAGCACT+TGG 0.472165 7.4:-4245216 None:intergenic
TTTCTACTAATCACTGGTCT+TGG 0.477113 7.4:+4245024 MS.gene054421:CDS
AACAGTTTAGAGGAGATTGA+AGG 0.483257 7.4:-4245374 None:intergenic
CTACTCTTTCTACTAATCAC+TGG 0.492211 7.4:+4245018 MS.gene054421:CDS
ATTCCAATCCCAAGGATACA+TGG 0.495507 7.4:-4244974 None:intergenic
GTTGAGTGCAGAAATATTGA+AGG 0.498744 7.4:-4245319 None:intergenic
GGATTGGAATAGTTTAAAGA+TGG 0.503276 7.4:+4244987 MS.gene054421:CDS
ATAAGATCAGACTTAGGGTT+GGG 0.510350 7.4:+4245502 MS.gene054421:CDS
ATTCCCATAAGATCAGACTT+AGG 0.515395 7.4:+4245496 MS.gene054421:CDS
ATGATGATTGTGGTGACAAA+AGG 0.535496 7.4:+4245115 MS.gene054421:CDS
CAATCATCATTAGTGCTAAG+TGG 0.546790 7.4:-4245102 None:intergenic
TCATTAGTGCTAAGTGGGTG+AGG 0.560513 7.4:-4245096 None:intergenic
ATGCAATTTAGTTAATTAGG+AGG 0.566067 7.4:-4245612 None:intergenic
TTTAAACTATTCCAATCCCA+AGG 0.579737 7.4:-4244982 None:intergenic
CATAAGATCAGACTTAGGGT+TGG 0.583330 7.4:+4245501 MS.gene054421:CDS
AATCATCATTAGTGCTAAGT+GGG 0.586032 7.4:-4245101 None:intergenic
CAAGGAGATGCATTGATCAC+GGG 0.586885 7.4:-4245048 None:intergenic
TTACAAGCTTGTTAGCACTT+GGG 0.592605 7.4:-4245215 None:intergenic
GCATCTCCTTGTTCAAATTG+TGG 0.598062 7.4:+4245060 MS.gene054421:CDS
ACAAGGAGATGCATTGATCA+CGG 0.611816 7.4:-4245049 None:intergenic
ACTATGGGATTGAGTTGCAA+TGG 0.615433 7.4:+4245586 MS.gene054421:CDS
TTGAGTGCAGAAATATTGAA+GGG 0.632612 7.4:-4245318 None:intergenic
AAGTCACCACAATTTGAACA+AGG 0.634351 7.4:-4245066 None:intergenic
TAGAGTTCTTGTCAGCAACA+GGG 0.637502 7.4:+4245166 MS.gene054421:CDS
GTGTGTTTAAGTATATTGGG+TGG 0.649267 7.4:-4245243 None:intergenic
CTTAGCACTAATGATGATTG+TGG 0.667956 7.4:+4245105 MS.gene054421:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTAAAAAATGGAACTAT+GGG + chr7.4:4245571-4245590 MS.gene054421:CDS 20.0%
!!! TTTTAAGTAATGACAACAAA+GGG - chr7.4:4245463-4245482 None:intergenic 20.0%
! ACAAGTGTGTTTAAGTATAT+TGG - chr7.4:4245250-4245269 None:intergenic 25.0%
! CAAGTGTGTTTAAGTATATT+GGG - chr7.4:4245249-4245268 None:intergenic 25.0%
!!! CATTTTTTAAAAGGTTCATC+AGG - chr7.4:4245564-4245583 None:intergenic 25.0%
!!! CCATAGTTCCATTTTTTAAA+AGG - chr7.4:4245573-4245592 None:intergenic 25.0%
!!! CCTTTTAAAAAATGGAACTA+TGG + chr7.4:4245570-4245589 MS.gene054421:CDS 25.0%
!!! CTGATGAACCTTTTAAAAAA+TGG + chr7.4:4245562-4245581 MS.gene054421:CDS 25.0%
!!! CTTTTAAGTAATGACAACAA+AGG - chr7.4:4245464-4245483 None:intergenic 25.0%
AATCATCATTAGTGCTAAGT+GGG - chr7.4:4245104-4245123 None:intergenic 30.0%
GGATTGGAATAGTTTAAAGA+TGG + chr7.4:4244987-4245006 MS.gene054421:CDS 30.0%
TGATTAGTAGAAAGAGTAGA+AGG - chr7.4:4245017-4245036 None:intergenic 30.0%
TGTTATTCTTCTGATCTCAT+TGG + chr7.4:4245267-4245286 MS.gene054421:CDS 30.0%
TTGAGTGCAGAAATATTGAA+GGG - chr7.4:4245321-4245340 None:intergenic 30.0%
TTTAAACTATTCCAATCCCA+AGG - chr7.4:4244985-4245004 None:intergenic 30.0%
AACAGTTTAGAGGAGATTGA+AGG - chr7.4:4245377-4245396 None:intergenic 35.0%
AACCCTAAGTCTGATCTTAT+GGG - chr7.4:4245502-4245521 None:intergenic 35.0%
AAGTCACCACAATTTGAACA+AGG - chr7.4:4245069-4245088 None:intergenic 35.0%
ATAAGATCAGACTTAGGGTT+GGG + chr7.4:4245502-4245521 MS.gene054421:CDS 35.0%
ATGATGATTGTGGTGACAAA+AGG + chr7.4:4245115-4245134 MS.gene054421:CDS 35.0%
ATTCCCATAAGATCAGACTT+AGG + chr7.4:4245496-4245515 MS.gene054421:CDS 35.0%
CAATCATCATTAGTGCTAAG+TGG - chr7.4:4245105-4245124 None:intergenic 35.0%
CTACTCTTTCTACTAATCAC+TGG + chr7.4:4245018-4245037 MS.gene054421:CDS 35.0%
CTGTTTGAAGAACAGTTTAG+AGG - chr7.4:4245387-4245406 None:intergenic 35.0%
CTTAGCACTAATGATGATTG+TGG + chr7.4:4245105-4245124 MS.gene054421:CDS 35.0%
GTGTGTTTAAGTATATTGGG+TGG - chr7.4:4245246-4245265 None:intergenic 35.0%
GTTGAGTGCAGAAATATTGA+AGG - chr7.4:4245322-4245341 None:intergenic 35.0%
TAGACAGTTTGAGGAAAAAG+TGG + chr7.4:4245413-4245432 MS.gene054421:CDS 35.0%
TATTCTTCTGATCTCATTGG+TGG + chr7.4:4245270-4245289 MS.gene054421:CDS 35.0%
TTCCCATAAGATCAGACTTA+GGG + chr7.4:4245497-4245516 MS.gene054421:CDS 35.0%
TTGTACTGCTTACACTTGTT+TGG + chr7.4:4245527-4245546 MS.gene054421:CDS 35.0%
! TAGCACTTGGGTCAATTTTA+AGG - chr7.4:4245206-4245225 None:intergenic 35.0%
! TTTCTACTAATCACTGGTCT+TGG + chr7.4:4245024-4245043 MS.gene054421:CDS 35.0%
!! ATTACAAGCTTGTTAGCACT+TGG - chr7.4:4245219-4245238 None:intergenic 35.0%
!! TTACAAGCTTGTTAGCACTT+GGG - chr7.4:4245218-4245237 None:intergenic 35.0%
AAAAAGTGGAGGAGGGAAAT+GGG + chr7.4:4245427-4245446 MS.gene054421:CDS 40.0%
ACAGTTTGAGGAAAAAGTGG+AGG + chr7.4:4245416-4245435 MS.gene054421:CDS 40.0%
CAACCCTAAGTCTGATCTTA+TGG - chr7.4:4245503-4245522 None:intergenic 40.0%
CAGCATTTGTAGACAGTTTG+AGG + chr7.4:4245404-4245423 MS.gene054421:CDS 40.0%
CATAAGATCAGACTTAGGGT+TGG + chr7.4:4245501-4245520 MS.gene054421:CDS 40.0%
GCATCTCCTTGTTCAAATTG+TGG + chr7.4:4245060-4245079 MS.gene054421:CDS 40.0%
TACTGCTTACACTTGTTTGG+TGG + chr7.4:4245530-4245549 MS.gene054421:CDS 40.0%
TAGAGTTCTTGTCAGCAACA+GGG + chr7.4:4245166-4245185 MS.gene054421:CDS 40.0%
TTAGAGTTCTTGTCAGCAAC+AGG + chr7.4:4245165-4245184 MS.gene054421:CDS 40.0%
TTTGAGGAAAAAGTGGAGGA+GGG + chr7.4:4245420-4245439 MS.gene054421:CDS 40.0%
! ACAAGGAGATGCATTGATCA+CGG - chr7.4:4245052-4245071 None:intergenic 40.0%
! ACTATGGGATTGAGTTGCAA+TGG + chr7.4:4245586-4245605 MS.gene054421:CDS 40.0%
GAAAAAGTGGAGGAGGGAAA+TGG + chr7.4:4245426-4245445 MS.gene054421:CDS 45.0%
GTTTGAGGAAAAAGTGGAGG+AGG + chr7.4:4245419-4245438 MS.gene054421:CDS 45.0%
TCATTAGTGCTAAGTGGGTG+AGG - chr7.4:4245099-4245118 None:intergenic 45.0%
TCTTCTGATCTCATTGGTGG+AGG + chr7.4:4245273-4245292 MS.gene054421:CDS 45.0%
! CAAGGAGATGCATTGATCAC+GGG - chr7.4:4245051-4245070 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 4244976 4245623 4244976 ID=MS.gene054421
chr7.4 mRNA 4244976 4245623 4244976 ID=MS.gene054421.t1;Parent=MS.gene054421
chr7.4 exon 4244976 4245623 4244976 ID=MS.gene054421.t1.exon1;Parent=MS.gene054421.t1
chr7.4 CDS 4244976 4245623 4244976 ID=cds.MS.gene054421.t1;Parent=MS.gene054421.t1
Gene Sequence

>MS.gene054421

ATGTATCCTTGGGATTGGAATAGTTTAAAGATGGTAATCCTTCTACTCTTTCTACTAATCACTGGTCTTGGTCCCGTGATCAATGCATCTCCTTGTTCAAATTGTGGTGACTTTGAAGTTCCTCACCCACTTAGCACTAATGATGATTGTGGTGACAAAAGGTACAAAATTTACTGCAATAATGATAGCTTAGAGTTCTTGTCAGCAACAGGGACATACTATAAGATCCTTAAAATTGACCCAAGTGCTAACAAGCTTGTAATTAAACCACCCAATATACTTAAACACACTTGTTATTCTTCTGATCTCATTGGTGGAGGCTTAGTACTTGATGAAAGCTTACCCTTCAATATTTCTGCACTCAACACTGTCATGCTTCTAAATTGCTCTGATAATATCCTTCAATCTCCTCTAAACTGTTCTTCAAACAGCATTTGTAGACAGTTTGAGGAAAAAGTGGAGGAGGGAAATGGGTGTATGAACACCCTTTGTTGTCATTACTTAAAAGATTCAGTCATGAATTCCCATAAGATCAGACTTAGGGTTGGGAGTTGTACTGCTTACACTTGTTTGGTGGATTTCAAGCCTGATGAACCTTTTAAAAAATGGAACTATGGGATTGAGTTGCAATGGAAGCCTCCTAATTAA

Protein sequence

>MS.gene054421.t1

MYPWDWNSLKMVILLLFLLITGLGPVINASPCSNCGDFEVPHPLSTNDDCGDKRYKIYCNNDSLEFLSATGTYYKILKIDPSANKLVIKPPNILKHTCYSSDLIGGGLVLDESLPFNISALNTVMLLNCSDNILQSPLNCSSNSICRQFEEKVEEGNGCMNTLCCHYLKDSVMNSHKIRLRVGSCTAYTCLVDFKPDEPFKKWNYGIELQWKPPN