AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051324

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051324.t1 MTR_1g099010 48.223 197 50 1 24 168 21 217 1.83e-58 182
MS.gene051324.t1 MTR_4g094332 88.421 95 11 0 1 95 1 95 3.53e-55 174
MS.gene051324.t1 MTR_4g094332 95.890 73 3 0 96 168 152 224 4.92e-42 140
MS.gene051324.t1 MTR_4g094325 44.498 209 62 2 13 168 32 239 8.55e-54 171
MS.gene051324.t1 MTR_4g094328 86.316 95 13 0 1 95 1 95 4.02e-53 168
MS.gene051324.t1 MTR_4g094328 94.521 73 4 0 96 168 152 224 3.17e-41 138
MS.gene051324.t1 MTR_4g094330 86.316 95 13 0 1 95 1 95 4.02e-53 168
MS.gene051324.t1 MTR_4g094330 94.521 73 4 0 96 168 152 224 3.17e-41 138
MS.gene051324.t1 MTR_0119s0010 46.409 181 50 1 30 163 27 207 2.22e-45 148
MS.gene051324.t1 MTR_0119s0020 46.409 181 50 1 30 163 27 207 2.22e-45 148
MS.gene051324.t1 MTR_2g008110 46.409 181 50 1 30 163 27 207 2.35e-45 148
MS.gene051324.t1 MTR_6g034975 60.000 80 31 1 16 95 5 83 6.10e-27 101
MS.gene051324.t1 MTR_6g034975 64.935 77 27 0 92 168 134 210 1.20e-23 92.8
MS.gene051324.t1 MTR_4g094335 59.036 83 30 1 13 95 20 98 1.65e-25 98.2
MS.gene051324.t1 MTR_4g094335 57.353 68 29 0 96 163 158 225 3.29e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051324.t1 AT3G43660 47.895 190 50 2 28 168 9 198 1.74e-55 173
MS.gene051324.t1 AT1G21140 45.408 196 56 2 24 168 5 200 4.30e-53 167
MS.gene051324.t1 AT1G76800 46.842 190 54 1 26 168 7 196 9.01e-53 166
MS.gene051324.t1 AT3G43630 45.550 191 55 2 24 165 4 194 9.96e-53 166
MS.gene051324.t1 AT3G25190 67.949 78 23 1 18 95 9 84 8.58e-30 108
MS.gene051324.t1 AT3G25190 58.228 79 33 0 90 168 139 217 4.47e-12 62.4

Find 44 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCTATGGCCATTACTTT+TGG 0.296297 4.1:-19283422 MS.gene051324:CDS
ACCAAATTGATTGGCACTAC+TGG 0.296615 4.1:-19283395 MS.gene051324:CDS
TGTGGTTAGGTTTGCATTGT+TGG 0.312190 4.1:-19283522 MS.gene051324:CDS
TCCAGTAGTGCCAATCAATT+TGG 0.330163 4.1:+19283394 None:intergenic
TTTGGTTTCACCAAATTGAT+TGG 0.341339 4.1:-19283404 MS.gene051324:CDS
TATCTCAATCTCAAAATTGC+TGG 0.375338 4.1:+19287467 None:intergenic
AGGTTTGCATTGTTGGTGTT+TGG 0.379958 4.1:-19283515 MS.gene051324:CDS
TGGCTTAGAGCAGCCATGTT+AGG 0.393100 4.1:-19287372 MS.gene051324:CDS
GTTTGGAGGAGTGGGTGCAT+TGG 0.412614 4.1:-19283498 MS.gene051324:CDS
ACCGTTGCGTCGTTGATGAT+TGG 0.428147 4.1:-19287330 MS.gene051324:CDS
AGAGTACTACTGACATGAAT+AGG 0.444320 4.1:+19287444 None:intergenic
GGTTTCGCTGGTTTAGTAGC+AGG 0.462292 4.1:-19287264 MS.gene051324:CDS
CAACATTGATTACTTTCAAA+GGG 0.466059 4.1:-19287400 MS.gene051324:CDS
ATGCTCCGAGCTGGTTTCGC+TGG 0.471108 4.1:-19287276 MS.gene051324:CDS
TTGTTGGTGTTTGGAGGAGT+GGG 0.472009 4.1:-19283506 MS.gene051324:CDS
AACGGTGATTAGTCCATCAT+TGG 0.472628 4.1:+19287347 None:intergenic
GTGTTGTTGCTGCTGTGGTT+AGG 0.476689 4.1:-19283535 MS.gene051324:CDS
GGAGGAGTGGGTGCATTGGT+TGG 0.478561 4.1:-19283494 MS.gene051324:CDS
TCTTGTGTTAGAGTTCTAAT+CGG 0.486108 4.1:-19283452 MS.gene051324:CDS
ACAACATTGATTACTTTCAA+AGG 0.489039 4.1:-19287401 MS.gene051324:CDS
TTTGCATTGTTGGTGTTTGG+AGG 0.490032 4.1:-19283512 MS.gene051324:CDS
GGAGCTTGTAGCATGGCAAT+TGG 0.492067 4.1:-19287243 MS.gene051324:intron
AAAGAATCACAAAATCAGAA+TGG 0.497688 4.1:-19283558 MS.gene051324:CDS
AAGAATCACAAAATCAGAAT+GGG 0.499997 4.1:-19283557 MS.gene051324:CDS
TTCAATATGGCCTCCCACCT+TGG 0.500245 4.1:-19287513 None:intergenic
ATTGTTGGTGTTTGGAGGAG+TGG 0.501761 4.1:-19283507 MS.gene051324:CDS
ATCTCAATCTCAAAATTGCT+GGG 0.516463 4.1:+19287468 None:intergenic
GCGTCGTTGATGATTGGTGT+TGG 0.527815 4.1:-19287324 MS.gene051324:CDS
AATGGGTGTTGTTGCTGCTG+TGG 0.531269 4.1:-19283540 MS.gene051324:CDS
TAAATGCAGCTGCTAGCAAT+GGG 0.531631 4.1:+19283582 None:intergenic
ATAAATGCAGCTGCTAGCAA+TGG 0.533922 4.1:+19283581 None:intergenic
TTTGGTGAAACCAAAAGTAA+TGG 0.542621 4.1:+19283412 None:intergenic
TCCATCATTGGCTCCTAACA+TGG 0.543290 4.1:+19287359 None:intergenic
AGTTCTAATCGGAGGTTGGA+TGG 0.545830 4.1:-19283441 MS.gene051324:CDS
ACCAATCATCAACGACGCAA+CGG 0.550619 4.1:+19287329 None:intergenic
TTAGAGTTCTAATCGGAGGT+TGG 0.557542 4.1:-19283445 MS.gene051324:CDS
AATCGGAGGTTGGATGGCTA+TGG 0.558633 4.1:-19283435 MS.gene051324:CDS
ATCAAAGTAATGCTCCGAGC+TGG 0.567968 4.1:-19287285 MS.gene051324:CDS
GCCATGTTAGGAGCCAATGA+TGG 0.577904 4.1:-19287360 MS.gene051324:CDS
TGTGTTAGAGTTCTAATCGG+AGG 0.592866 4.1:-19283449 MS.gene051324:CDS
ATTACTTTCAAAGGGCACAA+TGG 0.615521 4.1:-19287392 MS.gene051324:CDS
CTAAACCAGCGAAACCAGCT+CGG 0.629821 4.1:+19287271 None:intergenic
AGTAGCAGGAGCTTGTAGCA+TGG 0.637161 4.1:-19287250 MS.gene051324:CDS
CTAACACAAGACCTCATCAG+AGG 0.675054 4.1:+19283464 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATGATATTAAAATAATT+TGG + chr4.1:19285835-19285854 None:intergenic 10.0%
!! TCATGATATTAAAATAATTT+GGG + chr4.1:19285834-19285853 None:intergenic 10.0%
!! ATATATGTTATGGAAATTTA+TGG + chr4.1:19286327-19286346 None:intergenic 15.0%
!! ATTACCTATTATGAAATATT+TGG + chr4.1:19286158-19286177 None:intergenic 15.0%
!! ATTACTAAACTTAATAAGAT+GGG - chr4.1:19285560-19285579 MS.gene051324:intron 15.0%
!!! ATAGAGAATATTAATGATTT+TGG + chr4.1:19285764-19285783 None:intergenic 15.0%
!!! GTGTTATATTTAATTTTATG+TGG + chr4.1:19285609-19285628 None:intergenic 15.0%
!! AAACTTAACTTACTAAGTAA+TGG + chr4.1:19284674-19284693 None:intergenic 20.0%
!! AACACCAAATATTTCATAAT+AGG - chr4.1:19286151-19286170 MS.gene051324:intron 20.0%
!! AATACATTGAAGACATTATT+AGG + chr4.1:19285168-19285187 None:intergenic 20.0%
!! AATACTAAATCACATAAATC+CGG - chr4.1:19284608-19284627 MS.gene051324:intron 20.0%
!! AATATGTTAGATTTAACATG+TGG + chr4.1:19286097-19286116 None:intergenic 20.0%
!! AATCAATGTTATGATTCATT+AGG - chr4.1:19284349-19284368 MS.gene051324:intron 20.0%
!! ATACATTGAAGACATTATTA+GGG + chr4.1:19285167-19285186 None:intergenic 20.0%
!! ATTCAATGTATGATATATGT+TGG + chr4.1:19285493-19285512 None:intergenic 20.0%
!! CATGATAATATGAAAAGTAA+TGG + chr4.1:19285872-19285891 None:intergenic 20.0%
!! CATTACTAAACTTAATAAGA+TGG - chr4.1:19285559-19285578 MS.gene051324:intron 20.0%
!! CCATAACATATATTCATAAA+AGG - chr4.1:19286334-19286353 MS.gene051324:intron 20.0%
!! GTTAAATATTGTTATAGCAA+AGG + chr4.1:19285429-19285448 None:intergenic 20.0%
!! GTTTATGTTAAATTTACATG+TGG + chr4.1:19286282-19286301 None:intergenic 20.0%
!! TAAATCTGTAAAATACTGTT+AGG + chr4.1:19284121-19284140 None:intergenic 20.0%
!! TAAATTGTCAAAATATCAAG+TGG + chr4.1:19285965-19285984 None:intergenic 20.0%
!! TATAAAAGAAAAGTTGAAGT+TGG + chr4.1:19285465-19285484 None:intergenic 20.0%
!! TATAATGCATTATAAATGCT+TGG + chr4.1:19284292-19284311 None:intergenic 20.0%
!!! AACAGTATTTTACAGATTTA+TGG - chr4.1:19284121-19284140 MS.gene051324:intron 20.0%
!!! ATTTAGTATTTTGAAAATGG+TGG + chr4.1:19284598-19284617 None:intergenic 20.0%
!!! CCTTTTATGAATATATGTTA+TGG + chr4.1:19286337-19286356 None:intergenic 20.0%
!!! GTGATTTAGTATTTTGAAAA+TGG + chr4.1:19284601-19284620 None:intergenic 20.0%
!!! TTTAGTATTTTGAAAATGGT+GGG + chr4.1:19284597-19284616 None:intergenic 20.0%
! AAAGAATCACAAAATCAGAA+TGG - chr4.1:19287337-19287356 MS.gene051324:CDS 25.0%
! AAGAATCACAAAATCAGAAT+GGG - chr4.1:19287338-19287357 MS.gene051324:CDS 25.0%
! ACTCTTAAATTCTCATGAAA+AGG + chr4.1:19284842-19284861 None:intergenic 25.0%
! ATAACTTACACATAGAATCT+CGG - chr4.1:19284758-19284777 MS.gene051324:intron 25.0%
! ATTATAAATGCTTGGCATTT+GGG + chr4.1:19284284-19284303 None:intergenic 25.0%
! CAAGATATACAACAAATCTT+TGG + chr4.1:19283885-19283904 None:intergenic 25.0%
! CTCTTAAATTCTCATGAAAA+GGG + chr4.1:19284841-19284860 None:intergenic 25.0%
! TATGATTTACTTGTGACTAA+TGG + chr4.1:19286427-19286446 None:intergenic 25.0%
! TGTTGTATATCTTGATTGTT+GGG - chr4.1:19283891-19283910 MS.gene051324:intron 25.0%
! TTGAAACCTGAAAAGATTAA+TGG - chr4.1:19283929-19283948 MS.gene051324:intron 25.0%
! TTGTTGTATATCTTGATTGT+TGG - chr4.1:19283890-19283909 MS.gene051324:intron 25.0%
!! ACAACATTGATTACTTTCAA+AGG - chr4.1:19283494-19283513 MS.gene051324:CDS 25.0%
!! ACACAAAAACAACACAATTT+TGG + chr4.1:19284240-19284259 None:intergenic 25.0%
!! ATTGGTGATCAATTAGATTT+GGG - chr4.1:19285129-19285148 MS.gene051324:intron 25.0%
!! CAACATTGATTACTTTCAAA+GGG - chr4.1:19283495-19283514 MS.gene051324:CDS 25.0%
!! CTAAAGTGATTCCATTATTT+TGG - chr4.1:19284555-19284574 MS.gene051324:intron 25.0%
!!! ATAACATTGATTCAAGTCAA+AGG + chr4.1:19284341-19284360 None:intergenic 25.0%
!!! TAACATTGATTCAAGTCAAA+GGG + chr4.1:19284340-19284359 None:intergenic 25.0%
!!! TGTAAGTTGATATATGTTCA+TGG + chr4.1:19285796-19285815 None:intergenic 25.0%
!!! TTAGTATTTTGAAAATGGTG+GGG + chr4.1:19284596-19284615 None:intergenic 25.0%
AAATCGAACCTAAAGATTGT+TGG - chr4.1:19286446-19286465 MS.gene051324:intron 30.0%
AATTCTCATAGCTATGTCTA+TGG - chr4.1:19285299-19285318 MS.gene051324:intron 30.0%
ACTGTATATTGGATGCAATA+AGG - chr4.1:19284022-19284041 MS.gene051324:intron 30.0%
ATACACTTGAATCAATGCTA+TGG - chr4.1:19284399-19284418 MS.gene051324:intron 30.0%
ATACACTTGAATCAATGTCA+TGG - chr4.1:19284458-19284477 MS.gene051324:intron 30.0%
ATCCAATATACAGTTATTGG+TGG + chr4.1:19284016-19284035 None:intergenic 30.0%
ATCTCAATCTCAAAATTGCT+GGG + chr4.1:19283430-19283449 None:intergenic 30.0%
ATTTGACGAAATGATTGACA+AGG + chr4.1:19285216-19285235 None:intergenic 30.0%
CAAAAGTTGTAACTCTTCAT+GGG + chr4.1:19284532-19284551 None:intergenic 30.0%
CAAATCTAATTGATCACCAA+TGG + chr4.1:19285130-19285149 None:intergenic 30.0%
CACTTGTTCTAATGAATAGT+TGG + chr4.1:19284735-19284754 None:intergenic 30.0%
CATTATAAATGCTTGGCATT+TGG + chr4.1:19284285-19284304 None:intergenic 30.0%
CTTACAAGTCTCTTTATCAA+TGG + chr4.1:19284786-19284805 None:intergenic 30.0%
GACATATAGCATATATTTGC+TGG + chr4.1:19284947-19284966 None:intergenic 30.0%
GTGTATATGATCATAACCTT+TGG + chr4.1:19284385-19284404 None:intergenic 30.0%
GTGTATATGGTTGTAACATT+TGG + chr4.1:19284444-19284463 None:intergenic 30.0%
TATCTCAATCTCAAAATTGC+TGG + chr4.1:19283431-19283450 None:intergenic 30.0%
TATGCTATATGTCCATTAAG+AGG - chr4.1:19284954-19284973 MS.gene051324:intron 30.0%
TCCAATATACAGTTATTGGT+GGG + chr4.1:19284015-19284034 None:intergenic 30.0%
TGCATCCAATATACAGTTAT+TGG + chr4.1:19284019-19284038 None:intergenic 30.0%
TGGGAATCATTCATATACAT+TGG + chr4.1:19284513-19284532 None:intergenic 30.0%
TGTAAGATGACACATTAAGA+AGG - chr4.1:19284800-19284819 MS.gene051324:intron 30.0%
TTGGACCATAAGAAGAAAAA+GGG + chr4.1:19284994-19285013 None:intergenic 30.0%
TTTGGTGAAACCAAAAGTAA+TGG + chr4.1:19287486-19287505 None:intergenic 30.0%
! ATGATGATGAAAGTAGTGAA+AGG - chr4.1:19283758-19283777 MS.gene051324:intron 30.0%
! CATTGGTGATCAATTAGATT+TGG - chr4.1:19285128-19285147 MS.gene051324:intron 30.0%
! GATTTAGAGTAAGAGAATGA+GGG - chr4.1:19284182-19284201 MS.gene051324:intron 30.0%
! GCATAACCATTAATCTTTTC+AGG + chr4.1:19283938-19283957 None:intergenic 30.0%
! TACATTTTCTTCCATGACTA+CGG + chr4.1:19286377-19286396 None:intergenic 30.0%
! TCTTGTGTTAGAGTTCTAAT+CGG - chr4.1:19287443-19287462 MS.gene051324:CDS 30.0%
! TGATGATGAAAGTAGTGAAA+GGG - chr4.1:19283759-19283778 MS.gene051324:intron 30.0%
! TGATTTAGAGTAAGAGAATG+AGG - chr4.1:19284181-19284200 MS.gene051324:intron 30.0%
! TTTGGTTTCACCAAATTGAT+TGG - chr4.1:19287491-19287510 MS.gene051324:CDS 30.0%
!! AACATTGATTCAAGTCAAAG+GGG + chr4.1:19284339-19284358 None:intergenic 30.0%
!! GACATTGATTCAAGTGTATA+TGG + chr4.1:19284457-19284476 None:intergenic 30.0%
!! GTTGGTGATTTCCAAAATAA+TGG + chr4.1:19284569-19284588 None:intergenic 30.0%
!! TAGTATTTTGAAAATGGTGG+GGG + chr4.1:19284595-19284614 None:intergenic 30.0%
AAATCTGTTGGAACTTGTGT+TGG - chr4.1:19283792-19283811 MS.gene051324:intron 35.0%
AACACAAGTTCCAACAGATT+TGG + chr4.1:19283793-19283812 None:intergenic 35.0%
AAGATTGTTGGAACTTGTGT+TGG - chr4.1:19286458-19286477 MS.gene051324:intron 35.0%
AATCTGTTGGAACTTGTGTT+GGG - chr4.1:19283793-19283812 MS.gene051324:intron 35.0%
AATTCCATGCCTTCAGATAA+TGG - chr4.1:19284632-19284651 MS.gene051324:intron 35.0%
ACCATAAGAAGAAAAAGGGT+AGG + chr4.1:19284990-19285009 None:intergenic 35.0%
ACCTATGTGAGAATGAAGTT+TGG + chr4.1:19284890-19284909 None:intergenic 35.0%
AGATTGTTGGAACTTGTGTT+GGG - chr4.1:19286459-19286478 MS.gene051324:intron 35.0%
AGTCATCTTCATCATCTTCT+TGG + chr4.1:19283839-19283858 None:intergenic 35.0%
ATCGAACCTAAAGGTTAGTA+TGG + chr4.1:19283987-19284006 None:intergenic 35.0%
ATCTAATTGATCACCAATGG+TGG + chr4.1:19285127-19285146 None:intergenic 35.0%
ATGATCATAACCTTTGGCAA+AGG + chr4.1:19284379-19284398 None:intergenic 35.0%
ATGTAAACTCAAAGCGTATG+GGG - chr4.1:19283958-19283977 MS.gene051324:intron 35.0%
CAATATACAGTTATTGGTGG+GGG + chr4.1:19284013-19284032 None:intergenic 35.0%
CACAAGTTCCAACAATCTTT+AGG + chr4.1:19286457-19286476 None:intergenic 35.0%
CATGTAAACTCAAAGCGTAT+GGG - chr4.1:19283957-19283976 MS.gene051324:intron 35.0%
CCAAAAGTTGTAACTCTTCA+TGG + chr4.1:19284533-19284552 None:intergenic 35.0%
CCAATATACAGTTATTGGTG+GGG + chr4.1:19284014-19284033 None:intergenic 35.0%
CTCACATAGGTAGACTATAT+CGG - chr4.1:19284899-19284918 MS.gene051324:intron 35.0%
CTCATGCATTAGAGAATTGA+TGG - chr4.1:19284157-19284176 MS.gene051324:intron 35.0%
CTTGGACCATAAGAAGAAAA+AGG + chr4.1:19284995-19285014 None:intergenic 35.0%
GAAAAGGGATATATGACACA+AGG + chr4.1:19284826-19284845 None:intergenic 35.0%
GGTTGTAACATTTGGCAAAA+GGG + chr4.1:19284436-19284455 None:intergenic 35.0%
TCATTCCCAAATAGTATGGT+AGG - chr4.1:19284698-19284717 MS.gene051324:intron 35.0%
TGAAGACATTATTAGGGATG+TGG + chr4.1:19285161-19285180 None:intergenic 35.0%
TGACAATAGATACATCCCAA+GGG + chr4.1:19285027-19285046 None:intergenic 35.0%
TGATCATAACCTTTGGCAAA+GGG + chr4.1:19284378-19284397 None:intergenic 35.0%
TGGTTGTAACATTTGGCAAA+AGG + chr4.1:19284437-19284456 None:intergenic 35.0%
TTGACAAGGAATGATGTCTT+TGG + chr4.1:19285202-19285221 None:intergenic 35.0%
TTGACAATAGATACATCCCA+AGG + chr4.1:19285028-19285047 None:intergenic 35.0%
TTGCTGGAATGCATACTTAT+GGG + chr4.1:19284931-19284950 None:intergenic 35.0%
TTTGCTGGAATGCATACTTA+TGG + chr4.1:19284932-19284951 None:intergenic 35.0%
! AGAGTACTACTGACATGAAT+AGG + chr4.1:19283454-19283473 None:intergenic 35.0%
! ATTACTTTCAAAGGGCACAA+TGG - chr4.1:19283503-19283522 MS.gene051324:CDS 35.0%
! CCATGAAGAGTTACAACTTT+TGG - chr4.1:19284530-19284549 MS.gene051324:intron 35.0%
! GACTAATGGCTGCAATTTTT+AGG + chr4.1:19286413-19286432 None:intergenic 35.0%
! TCAGCACTTGCATTTTCTAT+TGG - chr4.1:19287281-19287300 MS.gene051324:CDS 35.0%
! TCCTACCCTTTTTCTTCTTA+TGG - chr4.1:19284986-19285005 MS.gene051324:intron 35.0%
! TGTCATGGTTTGATGATAGT+TGG + chr4.1:19285354-19285373 None:intergenic 35.0%
!! CTGCTTGAAATGGATTTTGT+TGG + chr4.1:19287253-19287272 None:intergenic 35.0%
!!! ATTAGTTTTTGCTCCAAGGT+GGG + chr4.1:19283398-19283417 None:intergenic 35.0%
!!! TTCCATTAGTTTTTGCTCCA+AGG + chr4.1:19283402-19283421 None:intergenic 35.0%
ACCAAATTGATTGGCACTAC+TGG - chr4.1:19287500-19287519 MS.gene051324:CDS 40.0%
ACTTGAGTAGCATCAAGTCT+TGG + chr4.1:19285091-19285110 None:intergenic 40.0%
ACTTGATGCTACTCAAGTGT+GGG - chr4.1:19285093-19285112 MS.gene051324:intron 40.0%
AGAAGAAAAAGGGTAGGATG+AGG + chr4.1:19284984-19285003 None:intergenic 40.0%
AGCTTGCCTACCATACTATT+TGG + chr4.1:19284707-19284726 None:intergenic 40.0%
AGGAACTCATAGTATCCTGA+AGG + chr4.1:19284064-19284083 None:intergenic 40.0%
ATAAATGCAGCTGCTAGCAA+TGG + chr4.1:19287317-19287336 None:intergenic 40.0%
ATACTGTTAGGAGATACGCT+TGG + chr4.1:19284109-19284128 None:intergenic 40.0%
ATCTGTTGGAACTTGTGTTG+GGG - chr4.1:19283794-19283813 MS.gene051324:intron 40.0%
ATTGTTGGAACTTGTGTTGG+GGG - chr4.1:19286461-19286480 MS.gene051324:intron 40.0%
CACCTTGGAGCAAAAACTAA+TGG - chr4.1:19283397-19283416 MS.gene051324:CDS 40.0%
CCCCACCAATAACTGTATAT+TGG - chr4.1:19284011-19284030 MS.gene051324:intron 40.0%
CTAATGCATGAGTGAATTGC+TGG + chr4.1:19284149-19284168 None:intergenic 40.0%
CTTGAGTAGCATCAAGTCTT+GGG + chr4.1:19285090-19285109 None:intergenic 40.0%
GAACTCATAGTATCCTGAAG+GGG + chr4.1:19284062-19284081 None:intergenic 40.0%
GACAATAGATACATCCCAAG+GGG + chr4.1:19285026-19285045 None:intergenic 40.0%
GATACCATTATCTGAAGGCA+TGG + chr4.1:19284639-19284658 None:intergenic 40.0%
GATCATAACCTTTGGCAAAG+GGG + chr4.1:19284377-19284396 None:intergenic 40.0%
GATTGTTGGAACTTGTGTTG+GGG - chr4.1:19286460-19286479 MS.gene051324:intron 40.0%
GCATGTAAACTCAAAGCGTA+TGG - chr4.1:19283956-19283975 MS.gene051324:intron 40.0%
GCCAAACTTCATTCTCACAT+AGG - chr4.1:19284886-19284905 MS.gene051324:intron 40.0%
GCTTGCCTACCATACTATTT+GGG + chr4.1:19284706-19284725 None:intergenic 40.0%
GGAACTCATAGTATCCTGAA+GGG + chr4.1:19284063-19284082 None:intergenic 40.0%
GGAGAAATTGCCAAATCTGT+TGG - chr4.1:19283780-19283799 MS.gene051324:intron 40.0%
TAAATGCAGCTGCTAGCAAT+GGG + chr4.1:19287316-19287335 None:intergenic 40.0%
TCCAGTAGTGCCAATCAATT+TGG + chr4.1:19287504-19287523 None:intergenic 40.0%
TCGCTCATTCCCAAATAGTA+TGG - chr4.1:19284694-19284713 MS.gene051324:intron 40.0%
TGCTGGAATGCATACTTATG+GGG + chr4.1:19284930-19284949 None:intergenic 40.0%
TGGATGCAATAAGGAATGAC+CGG - chr4.1:19284031-19284050 MS.gene051324:intron 40.0%
TTGGAAAAACTCCTCTGATG+AGG - chr4.1:19287420-19287439 MS.gene051324:CDS 40.0%
! AACGGTGATTAGTCCATCAT+TGG + chr4.1:19283551-19283570 None:intergenic 40.0%
! ATCTGAAGGCATGGAATTTC+CGG + chr4.1:19284630-19284649 None:intergenic 40.0%
! ATGGCTATGGCCATTACTTT+TGG - chr4.1:19287473-19287492 MS.gene051324:CDS 40.0%
! GGATGAGGTTTTCCTCTTAA+TGG + chr4.1:19284969-19284988 None:intergenic 40.0%
! TCCAAGTACTTTTACCCCTT+GGG - chr4.1:19285009-19285028 MS.gene051324:intron 40.0%
! TGTGTTAGAGTTCTAATCGG+AGG - chr4.1:19287446-19287465 MS.gene051324:CDS 40.0%
! TTAGAGTTCTAATCGGAGGT+TGG - chr4.1:19287450-19287469 MS.gene051324:CDS 40.0%
!! AAAATGCAAGTGCTGATGCT+AGG + chr4.1:19287277-19287296 None:intergenic 40.0%
!! AAATGCAAGTGCTGATGCTA+GGG + chr4.1:19287276-19287295 None:intergenic 40.0%
!! CTCAATTGACGAAAGTGTCA+TGG + chr4.1:19285369-19285388 None:intergenic 40.0%
!!! CATTAGTTTTTGCTCCAAGG+TGG + chr4.1:19283399-19283418 None:intergenic 40.0%
AATGAAGTTTGGCCGCTTCA+CGG + chr4.1:19284879-19284898 None:intergenic 45.0%
ACCAATCATCAACGACGCAA+CGG + chr4.1:19283569-19283588 None:intergenic 45.0%
ATCAAAGTAATGCTCCGAGC+TGG - chr4.1:19283610-19283629 MS.gene051324:intron 45.0%
CGCGAGATACCATTATCTGA+AGG + chr4.1:19284644-19284663 None:intergenic 45.0%
CTAACACAAGACCTCATCAG+AGG + chr4.1:19287434-19287453 None:intergenic 45.0%
CTTGGCATTTGGGCATTAGT+TGG + chr4.1:19284274-19284293 None:intergenic 45.0%
GACTTGATGCTACTCAAGTG+TGG - chr4.1:19285092-19285111 MS.gene051324:intron 45.0%
GCATCAAGTCTTGGGTTCAA+TGG + chr4.1:19285082-19285101 None:intergenic 45.0%
GGGTTGCCATACTAACCTTT+AGG - chr4.1:19283978-19283997 MS.gene051324:intron 45.0%
GTTAGGAGATACGCTTGGTT+TGG + chr4.1:19286770-19286789 None:intergenic 45.0%
TCTGTTGGAACTTGTGTTGG+GGG - chr4.1:19283795-19283814 MS.gene051324:intron 45.0%
TCTTCCATGACTACGGTGAA+CGG + chr4.1:19286370-19286389 None:intergenic 45.0%
TGGTTGTGCAATACACGTCA+AGG + chr4.1:19284084-19284103 None:intergenic 45.0%
TTTACCGTTCACCGTAGTCA+TGG - chr4.1:19286363-19286382 MS.gene051324:intron 45.0%
! AGTTCTAATCGGAGGTTGGA+TGG - chr4.1:19287454-19287473 MS.gene051324:CDS 45.0%
! ATTGTTGGTGTTTGGAGGAG+TGG - chr4.1:19287388-19287407 MS.gene051324:CDS 45.0%
! GTCCAAGTACTTTTACCCCT+TGG - chr4.1:19285008-19285027 MS.gene051324:intron 45.0%
! TGAAAATGGTGGGGGATTGT+TGG + chr4.1:19284587-19284606 None:intergenic 45.0%
! TTGTTGGTGTTTGGAGGAGT+GGG - chr4.1:19287389-19287408 MS.gene051324:CDS 45.0%
!! TCCATCATTGGCTCCTAACA+TGG + chr4.1:19283539-19283558 None:intergenic 45.0%
!! TCCCAAGGGGTAAAAGTACT+TGG + chr4.1:19285013-19285032 None:intergenic 45.0%
AACCCTTGAACTCCGTGAAG+CGG - chr4.1:19284864-19284883 MS.gene051324:intron 50.0%
AATCGGAGGTTGGATGGCTA+TGG - chr4.1:19287460-19287479 MS.gene051324:CDS 50.0%
AGTAGCAGGAGCTTGTAGCA+TGG - chr4.1:19283645-19283664 MS.gene051324:intron 50.0%
ATTAGGCACCCCTTTGCCAA+AGG - chr4.1:19284366-19284385 MS.gene051324:intron 50.0%
CTAAACCAGCGAAACCAGCT+CGG + chr4.1:19283627-19283646 None:intergenic 50.0%
GATGCTAGGGCTGCTTGAAA+TGG + chr4.1:19287263-19287282 None:intergenic 50.0%
GCCATGTTAGGAGCCAATGA+TGG - chr4.1:19283535-19283554 MS.gene051324:CDS 50.0%
GGAGCTTGTAGCATGGCAAT+TGG - chr4.1:19283652-19283671 MS.gene051324:intron 50.0%
TGGCTTAGAGCAGCCATGTT+AGG - chr4.1:19283523-19283542 MS.gene051324:CDS 50.0%
! AGTTTTTGCTCCAAGGTGGG+AGG + chr4.1:19283395-19283414 None:intergenic 50.0%
! GTGGGTTGAGTTTCCACCAT+TGG - chr4.1:19285111-19285130 MS.gene051324:intron 50.0%
! GTGTTGTTGCTGCTGTGGTT+AGG - chr4.1:19287360-19287379 MS.gene051324:CDS 50.0%
! GTTAGGAGATACGCTTGGTG+TGG + chr4.1:19284104-19284123 None:intergenic 50.0%
!! AATGGGTGTTGTTGCTGCTG+TGG - chr4.1:19287355-19287374 MS.gene051324:CDS 50.0%
!! ACCGTTGCGTCGTTGATGAT+TGG - chr4.1:19283565-19283584 MS.gene051324:CDS 50.0%
!! GCGTCGTTGATGATTGGTGT+TGG - chr4.1:19283571-19283590 MS.gene051324:CDS 50.0%
!! GGTTTCGCTGGTTTAGTAGC+AGG - chr4.1:19283631-19283650 MS.gene051324:intron 50.0%
GTATCCTGAAGGGGATTCGC+CGG + chr4.1:19284053-19284072 None:intergenic 55.0%
GTTTGGAGGAGTGGGTGCAT+TGG - chr4.1:19287397-19287416 MS.gene051324:CDS 55.0%
TGGGGGCGAATCGAACCTAA+AGG + chr4.1:19283996-19284015 None:intergenic 55.0%
ATGACCGGCGAATCCCCTTC+AGG - chr4.1:19284046-19284065 MS.gene051324:intron 60.0%
GGCCGCTTCACGGAGTTCAA+GGG + chr4.1:19284869-19284888 None:intergenic 60.0%
TGGCCGCTTCACGGAGTTCA+AGG + chr4.1:19284870-19284889 None:intergenic 60.0%
! ATGCTCCGAGCTGGTTTCGC+TGG - chr4.1:19283619-19283638 MS.gene051324:intron 60.0%
! GGAGGAGTGGGTGCATTGGT+TGG - chr4.1:19287401-19287420 MS.gene051324:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 19283388 19287529 19283388 ID=MS.gene051324
chr4.1 mRNA 19283388 19287529 19283388 ID=MS.gene051324.t1;Parent=MS.gene051324
chr4.1 exon 19287244 19287529 19287244 ID=MS.gene051324.t1.exon1;Parent=MS.gene051324.t1
chr4.1 CDS 19287244 19287529 19287244 ID=cds.MS.gene051324.t1;Parent=MS.gene051324.t1
chr4.1 exon 19283388 19283608 19283388 ID=MS.gene051324.t1.exon2;Parent=MS.gene051324.t1
chr4.1 CDS 19283388 19283608 19283388 ID=cds.MS.gene051324.t1;Parent=MS.gene051324.t1
Gene Sequence

>MS.gene051324

ATGGCCTCCCACCTTGGAGCAAAAACTAATGGAATTTCACCCAGCAATTTTGAGATTGAGATACCTATTCATGTCAGTAGTACTCTCAAAATTGAAAAAGAGAGCAACAACATTGATTACTTTCAAAGGGCACAATGGCTTAGAGCAGCCATGTTAGGAGCCAATGATGGACTAATCACCGTTGCGTCGTTGATGATTGGTGTTGGAGCTATAAAAGAAGACATCAAAGTAATGCTCCGAGCTGGTTTCGCTGGTTTAGTAGCAGGAGCTTGTAGCATGGCAATTGGTGAGTTTGTGTCTGTTTATACTCAATATGACATTGAGATAGCTCAGATAAATAGAGAAAGTGAAGCTAATAATAATAATGATGATGATGATGAAAGTAGTGAAAGGGAGAAATTGCCAAATCTGTTGGAACTTGTGTTGGGGGTCTGCTGCAATAACTTGACCAAGAAGATGATGAAGATGACTATGATGAAGTGTGCACATAAATTCCAAAGATTTGTTGTATATCTTGATTGTTGGGAAGATGCTGTTGTACTTGAAACCTGAAAAGATTAATGGTTATGCATGTAAACTCAAAGCGTATGGGGTTGCCATACTAACCTTTAGGTTCGATTCGCCCCCACCAATAACTGTATATTGGATGCAATAAGGAATGACCGGCGAATCCCCTTCAGGATACTATGAGTTCCTTGACGTGTATTGCACAACCACACCAAGCGTATCTCCTAACAGTATTTTACAGATTTATGGTTCCAGCAATTCACTCATGCATTAGAGAATTGATGGATGATTTAGAGTAAGAGAATGAGGGAGAATTATGTTGTTAATTTAACAAAATTCTGTCCAAAATTGTGTTGTTTTTGTGTTTTTTGTGTATCCAACTAATGCCCAAATGCCAAGCATTTATAATGCATTATATGACTTTTCACATGAGAGAAAACACCCCTTTGACTTGAATCAATGTTATGATTCATTAGGCACCCCTTTGCCAAAGGTTATGATCATATACACTTGAATCAATGCTATGGTTCATTAAACACCCTTTTGCCAAATGTTACAACCATATACACTTGAATCAATGTCATGGTTCATTATCTATCAATAAATAATAATGTTCCAATGTATATGAATGATTCCCATGAAGAGTTACAACTTTTGGTTCTAAAGTGATTCCATTATTTTGGAAATCACCAACAATCCCCCACCATTTTCAAAATACTAAATCACATAAATCCGGAAATTCCATGCCTTCAGATAATGGTATCTCGCGATTTGAACCATTACTTAGTAAGTTAAGTTTTCGCTCATTCCCAAATAGTATGGTAGGCAAGCTTTGAACCAACTATTCATTAGAACAAGTGTGCATAACTTACACATAGAATCTCGGTACCATTGATAAAGAGACTTGTAAGATGACACATTAAGAAGGCCTTGTGTCATATATCCCTTTTCATGAGAATTTAAGAGTCAAACCCTTGAACTCCGTGAAGCGGCCAAACTTCATTCTCACATAGGTAGACTATATCGGAAATGCCCCATAAGTATGCATTCCAGCAAATATATGCTATATGTCCATTAAGAGGAAAACCTCATCCTACCCTTTTTCTTCTTATGGTCCAAGTACTTTTACCCCTTGGGATGTATCTATTGTCAAGTACTTCAATCACTCTAATAGTGTACTTTCACCATTGAACCCAAGACTTGATGCTACTCAAGTGTGGGTTGAGTTTCCACCATTGGTGATCAATTAGATTTGGGTTTGTACCACATCCCTAATAATGTCTTCAATGTATTCAACATCATTTCCAAAGACATCATTCCTTGTCAATCATTTCGTCAAATAATTGCAAGATTTTCTGTTAACTTTTATAAACTTTGAATATTCAATTCTTCACATAGATTTTTAATTCTCATAGCTATGTCTATGGATCAACTTATATACTCTTGATAGTATTTACCAACTATCATCAAACCATGACACTTTCGTCAATTGAGTATATTGTAATAGTACACAAACTTTTATGTCATTACACCTTTGCTATAACAATATTTAACATGCATTTCAATTCCAACTTCAACTTTTCTTTTATATCTAGCCAACATATATCATACATTGAATGCATTAAACATACATAGCAAAATAAAACTAGATCATAAAAGAATGTCATTACTAAACTTAATAAGATGGGATTAAACATGTAAGTCATATACTCCACATAAAATTAAATATAACACAAAAATTTTGATTTTAGTTATCATATAACTAATACATATATCATAAATTCATTGTGTTAAATTCAAGTTTATGTTTAAATATGTTCAATGTTAAATATCAATAAACAGTCACATAAGAAAATAAACAATGAACCAAAATCATTAATATTCTCTATGACTTTCTTCCATGAACATATATCAACTTACAATAATTCAAAACATACCCAAATTATTTTAATATCATGATAAACAAGAAATTAACCATTACTTTTCATATTATCATGTACTTATGATATCAACACAATATTAATGATAATAATTTCTTACACAAAGTAAATAATATCATTAAAGCAACCACTTGATATTTTGACAATTTATCTACACTACTTTCATAAATAAAATAGTGTTTAAACATTCTTAACATATTACTATACTCATCAAAATTCATATACTTGAACAATTTCTAAATCATGTATATATATGTCACCACATGTTAAATCTAACATATTCATATTTACTTAGATACTATACACATGCATTTAGAACACCAAATATTTCATAATAGGTAATCACTTATATGCACATTATCTCATGTTTAAATTAACATCAAACAAATTTCAAACGATAAATTTCATACATATCAAAATTTATCATGCATATATTATCTACAACCACATGTAAATTTAACATAAACACATCACATATGAAATATTTATCCATAAATTTCCATAACATATATTCATAAAAGGAATTTGTTTACCGTTCACCGTAGTCATGGAAGAAAATGTAAGCTTTCATCTCTCCTAAAAATTGCAGCCATTAGTCACAAGTAAATCATAAATCGAACCTAAAGATTGTTGGAACTTGTGTTGGGGGTCTGCTGCAATAACTTGACCAAGAAGATGATGAAGATGACTATGATGAAGTGTGCACATAAATTCCAAAGATTTGTTGTATATCTTGATTGTTGGGAAGATGCTGTTGTACTTGAAACCTGAAAAGATTAATGGTTATGCATGTAAACTCAAAGCGTATGGGGTTGCCATACTAACCTTTAGGTTCGATTCGCCCCCACCAATAACTGTATATTGGATGCAATAAGGAATGACCGGCGAATCCCCTTCAGGATACTATGAGTTCCTTGACGTGTATTGCACAACCAAACCAAGCGTATCTCCTAACAGTATTTTACAGATTTATGGTTCCAGCAATTCACTCATGCATTAGAGAATTGATGGATGATTTAGAGTAAGAGAATGAGGGAGAATTATGTTGTTAATTTAACAAAATTCTGTCCAAAATTGTGTTGTTTTTGTGTTTTTTGTGTATCCAACTAATGCCCAAATGCCAAGCATTTATAATGCATTATATGACTTTTCACATGAGAGAAAACACCCCTTTGACTTGAATCAATGTTATGATTCATTAGGCACCCCTTTGCCAAAGGTTATGATCATATACACTTGAATCAATGCTATGGTTCATTAAACACCCTTTTGCCAAATGTTACAACCATATACACTTGAATCAATGTCATGGTTCATTATCTATCAATAAATAATAATGTTCCAATGTATATGAATGATTCCCATGAAGAGTTACAACTTTTGGTTCTAAAGTGATTCCATTATTTTGGAAATCACCAACAAAATCCATTTCAAGCAGCCCTAGCATCAGCACTTGCATTTTCTATTGGTGCAGTGATCCCATTGCTAGCAGCTGCATTTATAAAGAATCACAAAATCAGAATGGGTGTTGTTGCTGCTGTGGTTAGGTTTGCATTGTTGGTGTTTGGAGGAGTGGGTGCATTGGTTGGAAAAACTCCTCTGATGAGGTCTTGTGTTAGAGTTCTAATCGGAGGTTGGATGGCTATGGCCATTACTTTTGGTTTCACCAAATTGATTGGCACTACTGGACTTTGA
Protein sequence

>MS.gene051324.t1

MASHLGAKTNGISPSNFEIEIPIHVSSTLKIEKESNNIDYFQRAQWLRAAMLGANDGLITVASLMIGVGAIKEDIKVMLRAGFAGLVAGACSMAIVIPLLAAAFIKNHKIRMGVVAAVVRFALLVFGGVGALVGKTPLMRSCVRVLIGGWMAMAITFGFTKLIGTTGL