Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35052.t1 | RHN62754.1 | 92 | 225 | 17 | 1 | 1 | 225 | 1 | 224 | 5.20E-94 | 354 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35052.t1 | Q9M2C0 | 61.6 | 198 | 66 | 2 | 29 | 225 | 10 | 198 | 3.4e-62 | 239.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35052.t1 | A0A396IER6 | 92.0 | 225 | 17 | 1 | 1 | 225 | 1 | 224 | 3.7e-94 | 354.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051324 | MS.gene35052 | 0.821126 | 4.76E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35052 | MS.gene006180 | PPI |
| MS.gene35052 | MS.gene60673 | PPI |
| MS.gene35052 | MS.gene48173 | PPI |
| MS.gene35052 | MS.gene000219 | PPI |
| MS.gene48173 | MS.gene35052 | PPI |
| MS.gene35052 | MS.gene67394 | PPI |
| MS.gene35052 | MS.gene066222 | PPI |
| MS.gene35052 | MS.gene59206 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35052.t1 | MTR_4g094332 | 91.556 | 225 | 18 | 1 | 1 | 225 | 1 | 224 | 2.42e-129 | 364 |
| MS.gene35052.t1 | MTR_4g094328 | 90.222 | 225 | 21 | 1 | 1 | 225 | 1 | 224 | 1.74e-126 | 357 |
| MS.gene35052.t1 | MTR_4g094330 | 90.222 | 225 | 21 | 1 | 1 | 225 | 1 | 224 | 1.74e-126 | 357 |
| MS.gene35052.t1 | MTR_1g099010 | 64.851 | 202 | 66 | 1 | 24 | 225 | 21 | 217 | 5.36e-89 | 261 |
| MS.gene35052.t1 | MTR_6g034975 | 66.500 | 200 | 62 | 2 | 26 | 225 | 16 | 210 | 8.71e-84 | 248 |
| MS.gene35052.t1 | MTR_4g094325 | 63.402 | 194 | 67 | 1 | 32 | 225 | 50 | 239 | 5.33e-82 | 244 |
| MS.gene35052.t1 | MTR_0119s0010 | 59.686 | 191 | 67 | 1 | 30 | 220 | 27 | 207 | 2.62e-71 | 216 |
| MS.gene35052.t1 | MTR_0119s0020 | 59.686 | 191 | 67 | 1 | 30 | 220 | 27 | 207 | 2.62e-71 | 216 |
| MS.gene35052.t1 | MTR_2g008110 | 59.686 | 191 | 67 | 1 | 30 | 220 | 27 | 207 | 3.48e-71 | 216 |
| MS.gene35052.t1 | MTR_4g094335 | 59.278 | 194 | 77 | 1 | 29 | 220 | 32 | 225 | 7.08e-70 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35052.t1 | AT3G43660 | 62.121 | 198 | 65 | 2 | 29 | 225 | 10 | 198 | 1.71e-89 | 262 |
| MS.gene35052.t1 | AT1G21140 | 60.000 | 205 | 70 | 2 | 24 | 225 | 5 | 200 | 6.56e-80 | 238 |
| MS.gene35052.t1 | AT3G43630 | 60.500 | 200 | 69 | 2 | 24 | 222 | 4 | 194 | 3.34e-79 | 236 |
| MS.gene35052.t1 | AT1G76800 | 61.500 | 200 | 67 | 1 | 26 | 225 | 7 | 196 | 1.45e-77 | 232 |
| MS.gene35052.t1 | AT3G25190 | 62.085 | 211 | 75 | 2 | 18 | 225 | 9 | 217 | 1.05e-71 | 218 |
Find 50 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGCTATGGCCATTACTTT+TGG | 0.296297 | 4.4:-20479849 | MS.gene35052:CDS |
| ACCAAATTGATTGGCACTAC+TGG | 0.296615 | 4.4:-20479822 | MS.gene35052:CDS |
| TGTGGTTAGGTTTGCATTGT+TGG | 0.312190 | 4.4:-20479949 | MS.gene35052:CDS |
| TCTTGTGTTAGAGTTCTAAT+TGG | 0.326616 | 4.4:-20479879 | MS.gene35052:CDS |
| TCCAGTAGTGCCAATCAATT+TGG | 0.330163 | 4.4:+20479821 | None:intergenic |
| TTTGGTTTCACCAAATTGAT+TGG | 0.341339 | 4.4:-20479831 | MS.gene35052:CDS |
| TATCTCAATCTCAAAATTGC+TGG | 0.375338 | 4.4:+20480430 | None:intergenic |
| AGGTTTGCATTGTTGGTGTT+TGG | 0.379958 | 4.4:-20479942 | MS.gene35052:CDS |
| AGGGCTGCTTGAAATGGATT+TGG | 0.388581 | 4.4:+20480068 | None:intergenic |
| TGGCTTAGAGCAGCCATGTT+AGG | 0.393100 | 4.4:-20480335 | MS.gene35052:CDS |
| GTTTGGAGGAGTGGGTGCAT+TGG | 0.412614 | 4.4:-20479925 | MS.gene35052:CDS |
| GATGCTAGGGCTGCTTGAAA+TGG | 0.413748 | 4.4:+20480062 | None:intergenic |
| ACCGTTGCGTCGTTGATGAT+TGG | 0.428147 | 4.4:-20480293 | MS.gene35052:CDS |
| AGAGTACTACTGACATGAAT+AGG | 0.444320 | 4.4:+20480407 | None:intergenic |
| TGTGTTAGAGTTCTAATTGG+AGG | 0.446628 | 4.4:-20479876 | MS.gene35052:CDS |
| GGTTTCGCTGGTTTAGTAGC+AGG | 0.462292 | 4.4:-20480227 | MS.gene35052:CDS |
| CAACATTGATTACTTTCAAA+GGG | 0.466059 | 4.4:-20480363 | MS.gene35052:CDS |
| ATGCTCCGAGCTGGTTTCGC+TGG | 0.471108 | 4.4:-20480239 | MS.gene35052:CDS |
| TTGTTGGTGTTTGGAGGAGT+GGG | 0.472009 | 4.4:-20479933 | MS.gene35052:CDS |
| AACGGTGATTAGTCCATCAT+TGG | 0.472628 | 4.4:+20480310 | None:intergenic |
| GGAGGAGTGGGTGCATTGGT+TGG | 0.478561 | 4.4:-20479921 | MS.gene35052:CDS |
| GTGTTGTAGCTGCTGTGGTT+AGG | 0.480340 | 4.4:-20479962 | MS.gene35052:CDS |
| ACAACATTGATTACTTTCAA+AGG | 0.489039 | 4.4:-20480364 | MS.gene35052:CDS |
| TTTGCATTGTTGGTGTTTGG+AGG | 0.490032 | 4.4:-20479939 | MS.gene35052:CDS |
| GGAGCTTGTAGCATGGCAAT+TGG | 0.492067 | 4.4:-20480206 | MS.gene35052:CDS |
| AAAGAATCACAAAATCAGAA+TGG | 0.497688 | 4.4:-20479985 | MS.gene35052:CDS |
| AAGAATCACAAAATCAGAAT+GGG | 0.499997 | 4.4:-20479984 | MS.gene35052:CDS |
| TTCAATATGGCCTCCCACCT+TGG | 0.500245 | 4.4:-20480476 | None:intergenic |
| ATTGTTGGTGTTTGGAGGAG+TGG | 0.501761 | 4.4:-20479934 | MS.gene35052:CDS |
| AAAATGCAAGTGCTGATGCT+AGG | 0.501770 | 4.4:+20480048 | None:intergenic |
| AATTGGAGGTTGGATGGCTA+TGG | 0.510325 | 4.4:-20479862 | MS.gene35052:CDS |
| ATCTCAATCTCAAAATTGCT+GGG | 0.516463 | 4.4:+20480431 | None:intergenic |
| AGTTCTAATTGGAGGTTGGA+TGG | 0.519543 | 4.4:-20479868 | MS.gene35052:CDS |
| TTAGAGTTCTAATTGGAGGT+TGG | 0.521312 | 4.4:-20479872 | MS.gene35052:CDS |
| GCGTCGTTGATGATTGGTGT+TGG | 0.527815 | 4.4:-20480287 | MS.gene35052:CDS |
| TAAATGCAGCTGCTAGCAAT+GGG | 0.531631 | 4.4:+20480009 | None:intergenic |
| ATAAATGCAGCTGCTAGCAA+TGG | 0.533922 | 4.4:+20480008 | None:intergenic |
| AAATGCAAGTGCTGATGCTA+GGG | 0.534060 | 4.4:+20480049 | None:intergenic |
| TTTGGTGAAACCAAAAGTAA+TGG | 0.542621 | 4.4:+20479839 | None:intergenic |
| TCCATCATTGGCTCCTAACA+TGG | 0.543290 | 4.4:+20480322 | None:intergenic |
| ACCAATCATCAACGACGCAA+CGG | 0.550619 | 4.4:+20480292 | None:intergenic |
| ATCAAAGTAATGCTCCGAGC+TGG | 0.567968 | 4.4:-20480248 | MS.gene35052:CDS |
| GCCATGTTAGGAGCCAATGA+TGG | 0.577904 | 4.4:-20480323 | MS.gene35052:CDS |
| ATGATGATGAAAGTAGTGAA+AGG | 0.610585 | 4.4:-20480100 | MS.gene35052:CDS |
| TGATGATGAAAGTAGTGAAA+GGG | 0.611622 | 4.4:-20480099 | MS.gene35052:CDS |
| AATGGGTGTTGTAGCTGCTG+TGG | 0.611775 | 4.4:-20479967 | MS.gene35052:CDS |
| ATTACTTTCAAAGGGCACAA+TGG | 0.615521 | 4.4:-20480355 | MS.gene35052:CDS |
| CTAAACCAGCGAAACCAGCT+CGG | 0.629821 | 4.4:+20480234 | None:intergenic |
| AGTAGCAGGAGCTTGTAGCA+TGG | 0.637161 | 4.4:-20480213 | MS.gene35052:CDS |
| CTAACACAAGACCTCATCAG+AGG | 0.675054 | 4.4:+20479891 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAGAATCACAAAATCAGAA+TGG | - | chr4.4:20480300-20480319 | MS.gene35052:CDS | 25.0% |
| ! | AAGAATCACAAAATCAGAAT+GGG | - | chr4.4:20480301-20480320 | MS.gene35052:CDS | 25.0% |
| !! | ACAACATTGATTACTTTCAA+AGG | - | chr4.4:20479921-20479940 | MS.gene35052:CDS | 25.0% |
| !! | CAACATTGATTACTTTCAAA+GGG | - | chr4.4:20479922-20479941 | MS.gene35052:CDS | 25.0% |
| ATCTCAATCTCAAAATTGCT+GGG | + | chr4.4:20479857-20479876 | None:intergenic | 30.0% | |
| TATCTCAATCTCAAAATTGC+TGG | + | chr4.4:20479858-20479877 | None:intergenic | 30.0% | |
| TTTGGTGAAACCAAAAGTAA+TGG | + | chr4.4:20480449-20480468 | None:intergenic | 30.0% | |
| ! | ATGATGATGAAAGTAGTGAA+AGG | - | chr4.4:20480185-20480204 | MS.gene35052:CDS | 30.0% |
| ! | TCTTGTGTTAGAGTTCTAAT+TGG | - | chr4.4:20480406-20480425 | MS.gene35052:CDS | 30.0% |
| ! | TGATGATGAAAGTAGTGAAA+GGG | - | chr4.4:20480186-20480205 | MS.gene35052:CDS | 30.0% |
| ! | TTTGGTTTCACCAAATTGAT+TGG | - | chr4.4:20480454-20480473 | MS.gene35052:CDS | 30.0% |
| ! | AGAGTACTACTGACATGAAT+AGG | + | chr4.4:20479881-20479900 | None:intergenic | 35.0% |
| ! | ATTACTTTCAAAGGGCACAA+TGG | - | chr4.4:20479930-20479949 | MS.gene35052:CDS | 35.0% |
| ! | TCAGCACTTGCATTTTCTAT+TGG | - | chr4.4:20480244-20480263 | MS.gene35052:CDS | 35.0% |
| ! | TGTGTTAGAGTTCTAATTGG+AGG | - | chr4.4:20480409-20480428 | MS.gene35052:CDS | 35.0% |
| ! | TTAGAGTTCTAATTGGAGGT+TGG | - | chr4.4:20480413-20480432 | MS.gene35052:CDS | 35.0% |
| !!! | ATTAGTTTTTGCTCCAAGGT+GGG | + | chr4.4:20479825-20479844 | None:intergenic | 35.0% |
| !!! | TTCCATTAGTTTTTGCTCCA+AGG | + | chr4.4:20479829-20479848 | None:intergenic | 35.0% |
| ACCAAATTGATTGGCACTAC+TGG | - | chr4.4:20480463-20480482 | MS.gene35052:CDS | 40.0% | |
| ATAAATGCAGCTGCTAGCAA+TGG | + | chr4.4:20480280-20480299 | None:intergenic | 40.0% | |
| CACCTTGGAGCAAAAACTAA+TGG | - | chr4.4:20479824-20479843 | MS.gene35052:CDS | 40.0% | |
| TAAATGCAGCTGCTAGCAAT+GGG | + | chr4.4:20480279-20480298 | None:intergenic | 40.0% | |
| TCCAGTAGTGCCAATCAATT+TGG | + | chr4.4:20480467-20480486 | None:intergenic | 40.0% | |
| TGTGGTTAGGTTTGCATTGT+TGG | - | chr4.4:20480336-20480355 | MS.gene35052:CDS | 40.0% | |
| TTGGAAAAACTCCTCTGATG+AGG | - | chr4.4:20480383-20480402 | MS.gene35052:CDS | 40.0% | |
| ! | AACGGTGATTAGTCCATCAT+TGG | + | chr4.4:20479978-20479997 | None:intergenic | 40.0% |
| ! | AGGTTTGCATTGTTGGTGTT+TGG | - | chr4.4:20480343-20480362 | MS.gene35052:CDS | 40.0% |
| ! | AGTTCTAATTGGAGGTTGGA+TGG | - | chr4.4:20480417-20480436 | MS.gene35052:CDS | 40.0% |
| ! | ATGGCTATGGCCATTACTTT+TGG | - | chr4.4:20480436-20480455 | MS.gene35052:CDS | 40.0% |
| ! | TTTGCATTGTTGGTGTTTGG+AGG | - | chr4.4:20480346-20480365 | MS.gene35052:CDS | 40.0% |
| !! | AAAATGCAAGTGCTGATGCT+AGG | + | chr4.4:20480240-20480259 | None:intergenic | 40.0% |
| !! | AAATGCAAGTGCTGATGCTA+GGG | + | chr4.4:20480239-20480258 | None:intergenic | 40.0% |
| !!! | CATTAGTTTTTGCTCCAAGG+TGG | + | chr4.4:20479826-20479845 | None:intergenic | 40.0% |
| AATTGGAGGTTGGATGGCTA+TGG | - | chr4.4:20480423-20480442 | MS.gene35052:CDS | 45.0% | |
| ACCAATCATCAACGACGCAA+CGG | + | chr4.4:20479996-20480015 | None:intergenic | 45.0% | |
| ATCAAAGTAATGCTCCGAGC+TGG | - | chr4.4:20480037-20480056 | MS.gene35052:CDS | 45.0% | |
| CTAACACAAGACCTCATCAG+AGG | + | chr4.4:20480397-20480416 | None:intergenic | 45.0% | |
| ! | AGGGCTGCTTGAAATGGATT+TGG | + | chr4.4:20480220-20480239 | None:intergenic | 45.0% |
| ! | ATTGTTGGTGTTTGGAGGAG+TGG | - | chr4.4:20480351-20480370 | MS.gene35052:CDS | 45.0% |
| ! | TTGTTGGTGTTTGGAGGAGT+GGG | - | chr4.4:20480352-20480371 | MS.gene35052:CDS | 45.0% |
| !! | TCCATCATTGGCTCCTAACA+TGG | + | chr4.4:20479966-20479985 | None:intergenic | 45.0% |
| AGTAGCAGGAGCTTGTAGCA+TGG | - | chr4.4:20480072-20480091 | MS.gene35052:CDS | 50.0% | |
| CTAAACCAGCGAAACCAGCT+CGG | + | chr4.4:20480054-20480073 | None:intergenic | 50.0% | |
| GATGCTAGGGCTGCTTGAAA+TGG | + | chr4.4:20480226-20480245 | None:intergenic | 50.0% | |
| GCCATGTTAGGAGCCAATGA+TGG | - | chr4.4:20479962-20479981 | MS.gene35052:CDS | 50.0% | |
| GGAGCTTGTAGCATGGCAAT+TGG | - | chr4.4:20480079-20480098 | MS.gene35052:CDS | 50.0% | |
| GTGTTGTAGCTGCTGTGGTT+AGG | - | chr4.4:20480323-20480342 | MS.gene35052:CDS | 50.0% | |
| TGGCTTAGAGCAGCCATGTT+AGG | - | chr4.4:20479950-20479969 | MS.gene35052:CDS | 50.0% | |
| ! | AATGGGTGTTGTAGCTGCTG+TGG | - | chr4.4:20480318-20480337 | MS.gene35052:CDS | 50.0% |
| ! | AGTTTTTGCTCCAAGGTGGG+AGG | + | chr4.4:20479822-20479841 | None:intergenic | 50.0% |
| !! | ACCGTTGCGTCGTTGATGAT+TGG | - | chr4.4:20479992-20480011 | MS.gene35052:CDS | 50.0% |
| !! | GCGTCGTTGATGATTGGTGT+TGG | - | chr4.4:20479998-20480017 | MS.gene35052:CDS | 50.0% |
| !! | GGTTTCGCTGGTTTAGTAGC+AGG | - | chr4.4:20480058-20480077 | MS.gene35052:CDS | 50.0% |
| GTTTGGAGGAGTGGGTGCAT+TGG | - | chr4.4:20480360-20480379 | MS.gene35052:CDS | 55.0% | |
| ! | ATGCTCCGAGCTGGTTTCGC+TGG | - | chr4.4:20480046-20480065 | MS.gene35052:CDS | 60.0% |
| ! | GGAGGAGTGGGTGCATTGGT+TGG | - | chr4.4:20480364-20480383 | MS.gene35052:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 20479815 | 20480492 | 20479815 | ID=MS.gene35052 |
| chr4.4 | mRNA | 20479815 | 20480492 | 20479815 | ID=MS.gene35052.t1;Parent=MS.gene35052 |
| chr4.4 | exon | 20479815 | 20480492 | 20479815 | ID=MS.gene35052.t1.exon1;Parent=MS.gene35052.t1 |
| chr4.4 | CDS | 20479815 | 20480492 | 20479815 | ID=cds.MS.gene35052.t1;Parent=MS.gene35052.t1 |
| Gene Sequence |
| Protein sequence |
