AlfalfaGEDB | MS.gene051369

M. sativa cultivar XinJiangDaYe / MS.gene051369

KEGG: K01623
GO: GO:0003674 GO:0003824 GO:0004332 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006165 GO:0006725 GO:0006753 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009117 GO:0009132 GO:0009135 GO:0009150 GO:0009179 GO:0009185 GO:0009259 GO:0009987 GO:0016052 GO:0016310 GO:0016829 GO:0016830 GO:0016832 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034641 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0046031 GO:0046034 GO:0046483 GO:0046939 GO:0055086 GO:0071704 GO:0072521 GO:1901135 GO:1901360 GO:1901564 GO:1901575
PFAM: PF00274.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051369.t1	PNX79792.1	93.9	131	8	0	1	131	142	272	1.50E-61	245.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051369.t1	P17784	84.0	131	21	0	1	131	228	358	6.7e-58	224.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051369.t1	A0A2K3LMN4	93.9	131	8	0	1	131	142	272	1.1e-61	245.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051369	MS.gene24640	0.882081	1.40E-70	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051369.t1	MTR_1g061050	82.443	131	23	0	1	131	228	358	7.60e-77	231
MS.gene051369.t1	MTR_5g069055	80.916	131	25	0	1	131	228	358	4.67e-75	227
MS.gene051369.t1	MTR_5g069050	79.389	131	27	0	1	131	254	384	1.38e-72	221
MS.gene051369.t1	MTR_5g096670	74.809	131	32	1	1	131	228	357	4.89e-62	194
MS.gene051369.t1	MTR_4g057670	53.030	132	58	3	1	131	272	400	2.36e-40	139
MS.gene051369.t1	MTR_4g071860	53.030	132	58	3	1	131	272	400	2.43e-40	139
MS.gene051369.t1	MTR_4g071880	51.515	132	60	3	1	131	258	386	7.51e-39	134
MS.gene051369.t1	MTR_1g109200	50.000	132	62	3	1	131	263	391	4.63e-38	132

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051369.t1	AT2G36460	83.206	131	22	0	1	131	137	267	7.99e-79	233
MS.gene051369.t1	AT2G36460	83.206	131	22	0	1	131	137	267	7.99e-79	233
MS.gene051369.t1	AT2G36460	83.206	131	22	0	1	131	228	358	1.15e-77	234
MS.gene051369.t1	AT3G52930	83.969	131	21	0	1	131	228	358	1.31e-77	233
MS.gene051369.t1	AT5G03690	80.303	132	25	1	1	131	136	267	1.73e-74	223
MS.gene051369.t1	AT5G03690	80.303	132	25	1	1	131	136	267	1.73e-74	223
MS.gene051369.t1	AT5G03690	80.303	132	25	1	1	131	228	359	1.96e-73	223
MS.gene051369.t1	AT5G03690	80.303	132	25	1	1	131	262	393	6.60e-73	223
MS.gene051369.t1	AT4G26530	74.046	131	34	0	1	131	228	358	1.14e-64	200
MS.gene051369.t1	AT4G26530	74.046	131	34	0	1	131	228	358	1.14e-64	200
MS.gene051369.t1	AT4G26530	74.046	131	34	0	1	131	228	358	1.14e-64	200
MS.gene051369.t1	AT4G26520	66.412	131	44	0	1	131	228	358	4.88e-56	178
MS.gene051369.t1	AT4G26520	66.412	131	44	0	1	131	225	355	5.20e-56	178
MS.gene051369.t1	AT4G26520	66.412	131	44	0	1	131	225	355	5.20e-56	178
MS.gene051369.t1	AT4G26520	66.667	129	43	0	1	129	228	356	1.60e-54	174
MS.gene051369.t1	AT2G21330	51.515	132	60	3	1	131	271	399	6.84e-40	137
MS.gene051369.t1	AT4G38970	51.515	132	60	3	1	131	270	398	8.29e-39	134
MS.gene051369.t1	AT2G01140	50.758	132	61	3	1	131	263	391	1.60e-37	131
MS.gene051369.t1	AT2G21330	46.212	132	57	4	1	131	271	389	1.70e-31	115

Find 46 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTCCCTTCAATTTGTTCA+TGG	0.195538	7.3:-10270340	None:intergenic
TGGGCACTTTCTTTCTCTTT+TGG	0.238095	7.3:+10270369	MS.gene051369:CDS
CTCAGGAAGCATTCATTTCT+AGG	0.287182	7.3:+10270454	MS.gene051369:CDS
GCAACTTTGGGAACTTATAA+AGG	0.304285	7.3:+10270495	MS.gene051369:CDS
GGCCTTGCAGCGAACCGTTC+CGG	0.326308	7.3:+10270248	MS.gene051369:CDS
CCTGCTGTTGTGTTCTTGTC+TGG	0.328998	7.3:+10270279	MS.gene051369:CDS
GGCTAACTCACAAGCAACTT+TGG	0.373884	7.3:+10270482	MS.gene051369:CDS
GTATATTCGGCTATAACTTC+AGG	0.378009	7.3:-10270219	None:intergenic
CAAGGCCTTAACGGTATATT+CGG	0.396094	7.3:-10270232	None:intergenic
CTCTGAGTCTCTCCATGTTA+AGG	0.403479	7.3:+10270542	MS.gene051369:CDS
GGTTCGCTGCAAGGCCTTAA+CGG	0.417489	7.3:-10270241	None:intergenic
AAGCATTCATTTCTAGGTGC+AGG	0.421155	7.3:+10270460	MS.gene051369:CDS
GCTAACTCACAAGCAACTTT+GGG	0.426606	7.3:+10270483	MS.gene051369:CDS
AGTACTCTCAAGGCATGGGC+TGG	0.441574	7.3:+10270408	MS.gene051369:CDS
CCAGACAAGAACACAACAGC+AGG	0.465132	7.3:-10270279	None:intergenic
TATAAAGGTGATTCTACCCT+TGG	0.470420	7.3:+10270510	MS.gene051369:CDS
CAATGCCATGAACAAATTGA+AGG	0.489466	7.3:+10270335	MS.gene051369:CDS
AGCCGGAACGGTTCGCTGCA+AGG	0.504681	7.3:-10270250	None:intergenic
GCTGTTGTGTTCTTGTCTGG+TGG	0.505060	7.3:+10270282	MS.gene051369:CDS
TATAGCCGAATATACCGTTA+AGG	0.505464	7.3:+10270227	MS.gene051369:CDS
TATATTCGGCTATAACTTCA+GGG	0.510081	7.3:-10270218	None:intergenic
GTACTCTCAAGGCATGGGCT+GGG	0.512522	7.3:+10270409	MS.gene051369:CDS
CCCCGGTTCAGATTCTCAAA+AGG	0.519538	7.3:+10270191	MS.gene051369:CDS
TCAATTTGTTCATGGCATTG+AGG	0.520997	7.3:-10270332	None:intergenic
AGCAGAGTACTCTCAAGGCA+TGG	0.525031	7.3:+10270403	MS.gene051369:CDS
TGAAAACATTAAGAAGGCTC+AGG	0.537239	7.3:+10270437	MS.gene051369:CDS
ACAACAGCAGGAACTGCAGC+CGG	0.552294	7.3:-10270267	None:intergenic
TGGTGGACAGAGTGAGGAGG+AGG	0.559419	7.3:+10270299	MS.gene051369:CDS
GAAAGATGAAAACATTAAGA+AGG	0.560338	7.3:+10270431	MS.gene051369:CDS
AGCAGGAACTGCAGCCGGAA+CGG	0.563737	7.3:-10270262	None:intergenic
AGCATTCATTTCTAGGTGCA+GGG	0.565780	7.3:+10270461	MS.gene051369:CDS
AAGCCCAACATGGTAACCCC+CGG	0.580471	7.3:+10270174	None:intergenic
GCAGAGTACTCTCAAGGCAT+GGG	0.580912	7.3:+10270404	MS.gene051369:CDS
AGTATTTGTAATCCTTAACA+TGG	0.585318	7.3:-10270554	None:intergenic
AATGCCATGAACAAATTGAA+GGG	0.590609	7.3:+10270336	MS.gene051369:CDS
AATTGAAGGGAAAGAAGCCG+TGG	0.606270	7.3:+10270349	MS.gene051369:CDS
CTTGTCTGGTGGACAGAGTG+AGG	0.610600	7.3:+10270293	MS.gene051369:CDS
CTCAGAGGCACCATCACCAA+GGG	0.635565	7.3:-10270526	None:intergenic
GGTGATTCTACCCTTGGTGA+TGG	0.637135	7.3:+10270516	MS.gene051369:CDS
ACTTCAGCAGAGTACTCTCA+AGG	0.637395	7.3:+10270398	MS.gene051369:CDS
AAAGAGAAAGAAAGTGCCCA+CGG	0.650023	7.3:-10270366	None:intergenic
GTCTGGTGGACAGAGTGAGG+AGG	0.667820	7.3:+10270296	MS.gene051369:CDS
ACTCAGAGGCACCATCACCA+AGG	0.668916	7.3:-10270527	None:intergenic
CTTAACATGGAGAGACTCAG+AGG	0.681234	7.3:-10270541	None:intergenic
ATTGAAGGGAAAGAAGCCGT+GGG	0.685142	7.3:+10270350	MS.gene051369:CDS
ATATTCGGCTATAACTTCAG+GGG	0.697227	7.3:-10270217	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGTATTTGTAATCCTTAACA+TGG	-	chr7.3:10270557-10270576	None:intergenic	25.0%
!	GAAAGATGAAAACATTAAGA+AGG	+	chr7.3:10270431-10270450	MS.gene051369:CDS	25.0%
	AATGCCATGAACAAATTGAA+GGG	+	chr7.3:10270336-10270355	MS.gene051369:CDS	30.0%
	TATATTCGGCTATAACTTCA+GGG	-	chr7.3:10270221-10270240	None:intergenic	30.0%
	ATATTCGGCTATAACTTCAG+GGG	-	chr7.3:10270220-10270239	None:intergenic	35.0%
	CAATGCCATGAACAAATTGA+AGG	+	chr7.3:10270335-10270354	MS.gene051369:CDS	35.0%
	GTATATTCGGCTATAACTTC+AGG	-	chr7.3:10270222-10270241	None:intergenic	35.0%
	TATAAAGGTGATTCTACCCT+TGG	+	chr7.3:10270510-10270529	MS.gene051369:CDS	35.0%
	TATAGCCGAATATACCGTTA+AGG	+	chr7.3:10270227-10270246	MS.gene051369:CDS	35.0%
	TGAAAACATTAAGAAGGCTC+AGG	+	chr7.3:10270437-10270456	MS.gene051369:CDS	35.0%
!	CTTTCCCTTCAATTTGTTCA+TGG	-	chr7.3:10270343-10270362	None:intergenic	35.0%
!	GCAACTTTGGGAACTTATAA+AGG	+	chr7.3:10270495-10270514	MS.gene051369:CDS	35.0%
!	TCAATTTGTTCATGGCATTG+AGG	-	chr7.3:10270335-10270354	None:intergenic	35.0%
!!	AACCTTTTGAGAATCTGAAC+CGG	-	chr7.3:10270196-10270215	None:intergenic	35.0%
	CAAGGCCTTAACGGTATATT+CGG	-	chr7.3:10270235-10270254	None:intergenic	40.0%
	GCTAACTCACAAGCAACTTT+GGG	+	chr7.3:10270483-10270502	MS.gene051369:CDS	40.0%
!	AAGCATTCATTTCTAGGTGC+AGG	+	chr7.3:10270460-10270479	MS.gene051369:CDS	40.0%
!	AGCATTCATTTCTAGGTGCA+GGG	+	chr7.3:10270461-10270480	MS.gene051369:CDS	40.0%
!	TGGGCACTTTCTTTCTCTTT+TGG	+	chr7.3:10270369-10270388	MS.gene051369:CDS	40.0%
!!	AAAGAGAAAGAAAGTGCCCA+CGG	-	chr7.3:10270369-10270388	None:intergenic	40.0%
!!	ACCTTTTGAGAATCTGAACC+GGG	-	chr7.3:10270195-10270214	None:intergenic	40.0%
!!	CTCAGGAAGCATTCATTTCT+AGG	+	chr7.3:10270454-10270473	MS.gene051369:CDS	40.0%
	AATTGAAGGGAAAGAAGCCG+TGG	+	chr7.3:10270349-10270368	MS.gene051369:CDS	45.0%
	ATTGAAGGGAAAGAAGCCGT+GGG	+	chr7.3:10270350-10270369	MS.gene051369:CDS	45.0%
	CTCTGAGTCTCTCCATGTTA+AGG	+	chr7.3:10270542-10270561	MS.gene051369:CDS	45.0%
	CTTAACATGGAGAGACTCAG+AGG	-	chr7.3:10270544-10270563	None:intergenic	45.0%
	GGCTAACTCACAAGCAACTT+TGG	+	chr7.3:10270482-10270501	MS.gene051369:CDS	45.0%
!	ACTTCAGCAGAGTACTCTCA+AGG	+	chr7.3:10270398-10270417	MS.gene051369:CDS	45.0%
!!	CCTTTTGAGAATCTGAACCG+GGG	-	chr7.3:10270194-10270213	None:intergenic	45.0%
!!	CTTTTGAGAATCTGAACCGG+GGG	-	chr7.3:10270193-10270212	None:intergenic	45.0%
	CCAGACAAGAACACAACAGC+AGG	-	chr7.3:10270282-10270301	None:intergenic	50.0%
	CCCCGGTTCAGATTCTCAAA+AGG	+	chr7.3:10270191-10270210	MS.gene051369:CDS	50.0%
	CCTGCTGTTGTGTTCTTGTC+TGG	+	chr7.3:10270279-10270298	MS.gene051369:CDS	50.0%
	GCTGTTGTGTTCTTGTCTGG+TGG	+	chr7.3:10270282-10270301	MS.gene051369:CDS	50.0%
	GGTGATTCTACCCTTGGTGA+TGG	+	chr7.3:10270516-10270535	MS.gene051369:CDS	50.0%
!	AGCAGAGTACTCTCAAGGCA+TGG	+	chr7.3:10270403-10270422	MS.gene051369:CDS	50.0%
!	GCAGAGTACTCTCAAGGCAT+GGG	+	chr7.3:10270404-10270423	MS.gene051369:CDS	50.0%
	ACAACAGCAGGAACTGCAGC+CGG	-	chr7.3:10270270-10270289	None:intergenic	55.0%
	AGTACTCTCAAGGCATGGGC+TGG	+	chr7.3:10270408-10270427	MS.gene051369:CDS	55.0%
	GGTTCGCTGCAAGGCCTTAA+CGG	-	chr7.3:10270244-10270263	None:intergenic	55.0%
	GTACTCTCAAGGCATGGGCT+GGG	+	chr7.3:10270409-10270428	MS.gene051369:CDS	55.0%
!	CTTGTCTGGTGGACAGAGTG+AGG	+	chr7.3:10270293-10270312	MS.gene051369:CDS	55.0%
!!	ACTCAGAGGCACCATCACCA+AGG	-	chr7.3:10270530-10270549	None:intergenic	55.0%
!!	CTCAGAGGCACCATCACCAA+GGG	-	chr7.3:10270529-10270548	None:intergenic	55.0%
	AGCAGGAACTGCAGCCGGAA+CGG	-	chr7.3:10270265-10270284	None:intergenic	60.0%
!	GTCTGGTGGACAGAGTGAGG+AGG	+	chr7.3:10270296-10270315	MS.gene051369:CDS	60.0%
!	TGGTGGACAGAGTGAGGAGG+AGG	+	chr7.3:10270299-10270318	MS.gene051369:CDS	60.0%
	AGCCGGAACGGTTCGCTGCA+AGG	-	chr7.3:10270253-10270272	None:intergenic	65.0%
	GGCCTTGCAGCGAACCGTTC+CGG	+	chr7.3:10270248-10270267	MS.gene051369:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	10270183	10270578	10270183	ID=MS.gene051369
chr7.3	mRNA	10270183	10270578	10270183	ID=MS.gene051369.t1;Parent=MS.gene051369
chr7.3	exon	10270183	10270578	10270183	ID=MS.gene051369.t1.exon1;Parent=MS.gene051369.t1
chr7.3	CDS	10270183	10270578	10270183	ID=cds.MS.gene051369.t1;Parent=MS.gene051369.t1

Gene Sequence

>MS.gene051369

ATGGTAACCCCCGGTTCAGATTCTCAAAAGGTTGCCCCTGAAGTTATAGCCGAATATACCGTTAAGGCCTTGCAGCGAACCGTTCCGGCTGCAGTTCCTGCTGTTGTGTTCTTGTCTGGTGGACAGAGTGAGGAGGAGGCAACACTCAACCTCAATGCCATGAACAAATTGAAGGGAAAGAAGCCGTGGGCACTTTCTTTCTCTTTTGGAAGAGCACTTCAGCAGAGTACTCTCAAGGCATGGGCTGGGAAAGATGAAAACATTAAGAAGGCTCAGGAAGCATTCATTTCTAGGTGCAGGGCTAACTCACAAGCAACTTTGGGAACTTATAAAGGTGATTCTACCCTTGGTGATGGTGCCTCTGAGTCTCTCCATGTTAAGGATTACAAATACTAA

Protein sequence

>MS.gene051369.t1

MVTPGSDSQKVAPEVIAEYTVKALQRTVPAAVPAVVFLSGGQSEEEATLNLNAMNKLKGKKPWALSFSFGRALQQSTLKAWAGKDENIKKAQEAFISRCRANSQATLGTYKGDSTLGDGASESLHVKDYKY