Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051689.t1 | XP_003589063.1 | 90.8 | 292 | 19 | 2 | 1 | 291 | 1 | 285 | 3.80E-113 | 417.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051689.t1 | G7ICB3 | 90.8 | 292 | 19 | 2 | 1 | 291 | 1 | 285 | 2.7e-113 | 417.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051689 | MS.gene24479 | 0.842839 | 2.02E-58 | -1.69E-46 |
| MS.gene051689 | MS.gene36612 | 0.8059 | 1.07E-49 | -1.69E-46 |
| MS.gene051689 | MS.gene44289 | 0.802593 | 5.21E-49 | -1.69E-46 |
| MS.gene051689 | MS.gene73753 | 0.803716 | 3.05E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051689.t1 | MTR_1g017990 | 91.096 | 292 | 18 | 2 | 1 | 291 | 1 | 285 | 0.0 | 534 |
| MS.gene051689.t1 | MTR_3g103190 | 42.672 | 232 | 96 | 8 | 1 | 207 | 1 | 220 | 4.86e-47 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051689.t1 | AT1G59590 | 39.683 | 189 | 103 | 5 | 22 | 202 | 7 | 192 | 1.51e-32 | 119 |
| MS.gene051689.t1 | AT1G10220 | 40.000 | 180 | 95 | 5 | 22 | 190 | 3 | 180 | 1.59e-25 | 101 |
| MS.gene051689.t1 | AT1G10220 | 37.696 | 191 | 95 | 5 | 22 | 190 | 3 | 191 | 5.39e-24 | 97.4 |
Find 40 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGGCCACGTGTGGAATA+AGG | 0.226117 | 1.3:+4800436 | None:intergenic |
| ATTTGTTGTGATAATGATTT+TGG | 0.226913 | 1.3:-4800098 | MS.gene051689:CDS |
| ATTCCATGAAATTCTCTTCT+TGG | 0.239938 | 1.3:+4800162 | None:intergenic |
| AAGAAAAGAGATTATGTTAG+AGG | 0.255949 | 1.3:-4799974 | MS.gene051689:CDS |
| TATCTGATTTAAATGATAAA+AGG | 0.270978 | 1.3:-4800396 | MS.gene051689:CDS |
| AGGTTTGAGTGAGAAGAAAA+TGG | 0.283239 | 1.3:-4799954 | MS.gene051689:CDS |
| TATCTTCTAGGGCTTGAATT+AGG | 0.299114 | 1.3:+4800484 | None:intergenic |
| CATAGATCATCATCATCTTC+TGG | 0.320316 | 1.3:+4800517 | None:intergenic |
| TCAAAATCACCACCACTTCT+TGG | 0.326218 | 1.3:+4799752 | None:intergenic |
| GACTTAGGACTCAAGCTTCT+TGG | 0.327238 | 1.3:+4800244 | None:intergenic |
| TTCCATGAAATTCTCTTCTT+GGG | 0.358637 | 1.3:+4800163 | None:intergenic |
| CCCTTATTCCACACGTGGCC+TGG | 0.364320 | 1.3:-4800437 | MS.gene051689:CDS |
| ATGAAATTCTCTTCTTGGGT+TGG | 0.385407 | 1.3:+4800167 | None:intergenic |
| AAGATGGAGTGAGAAGAAAA+TGG | 0.393067 | 1.3:-4799801 | MS.gene051689:CDS |
| TTGAATGTTCCACAAACAAA+AGG | 0.402250 | 1.3:+4800541 | None:intergenic |
| CCAGGCCACGTGTGGAATAA+GGG | 0.414462 | 1.3:+4800437 | None:intergenic |
| GATTTAATATCCTTCAAAGT+TGG | 0.427450 | 1.3:+4800010 | None:intergenic |
| AAAATCATTATCACAACAAA+TGG | 0.436688 | 1.3:+4800100 | None:intergenic |
| AACCCAAGAAGAGAATTTCA+TGG | 0.440286 | 1.3:-4800165 | MS.gene051689:CDS |
| AGAAAGATTATGTTAGAAGA+TGG | 0.449967 | 1.3:-4799817 | MS.gene051689:CDS |
| AACATTCACACAAGAAAAGT+TGG | 0.459312 | 1.3:-4799700 | MS.gene051689:CDS |
| AAAATGGTTACTACTAATGA+TGG | 0.460282 | 1.3:-4799785 | MS.gene051689:CDS |
| GTATGTTATTCCAACTTTGA+AGG | 0.503391 | 1.3:-4800020 | MS.gene051689:CDS |
| TGAATCATCAATTGTTGCTA+TGG | 0.523729 | 1.3:-4800200 | MS.gene051689:CDS |
| ATGGATGTAGTGAATAATGA+CGG | 0.534632 | 1.3:-4799935 | MS.gene051689:CDS |
| TGAATGTTCCACAAACAAAA+GGG | 0.542425 | 1.3:+4800542 | None:intergenic |
| AAAGTGCTGAAAATTTATCC+AGG | 0.555876 | 1.3:+4800419 | None:intergenic |
| AAATTTATCCAGGCCACGTG+TGG | 0.580635 | 1.3:+4800429 | None:intergenic |
| GGATTAGTTTCTCCAAGAAG+TGG | 0.582854 | 1.3:-4799764 | MS.gene051689:CDS |
| TGGATGTAGTGAATAATGAC+GGG | 0.588904 | 1.3:-4799934 | MS.gene051689:CDS |
| AAAAGATGGTGGTTAATGAA+GGG | 0.592112 | 1.3:-4799850 | MS.gene051689:CDS |
| GAATATGAAGAATATTGAGA+TGG | 0.602572 | 1.3:-4800143 | MS.gene051689:CDS |
| CGAACAAAAGAAATATCATG+AGG | 0.627048 | 1.3:+4800349 | None:intergenic |
| AAGCTTGAGTCCTAAGTCAA+CGG | 0.627738 | 1.3:-4800239 | MS.gene051689:CDS |
| TTAGTTTCTCCAAGAAGTGG+TGG | 0.628810 | 1.3:-4799761 | MS.gene051689:CDS |
| ACAAATGGCAAATAGAACAA+TGG | 0.651573 | 1.3:+4800115 | None:intergenic |
| GAACAAAAGAAATATCATGA+GGG | 0.654561 | 1.3:+4800350 | None:intergenic |
| AATAACCCTTATTCCACACG+TGG | 0.697457 | 1.3:-4800442 | MS.gene051689:CDS |
| TGTAGTGAATAATGACGGGG+TGG | 0.710681 | 1.3:-4799930 | MS.gene051689:CDS |
| GGATGTAGTGAATAATGACG+GGG | 0.789564 | 1.3:-4799933 | MS.gene051689:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCTGATTTAAATGATAAA+AGG | - | chr1.3:4799851-4799870 | MS.gene051689:CDS | 15.0% |
| !! | AAAATCATTATCACAACAAA+TGG | + | chr1.3:4800150-4800169 | None:intergenic | 20.0% |
| !! | AAGAAAAAAGATTATGTTAG+AGG | - | chr1.3:4800363-4800382 | MS.gene051689:CDS | 20.0% |
| !! | AGAAAAAAGATTATGTTAGA+GGG | - | chr1.3:4800364-4800383 | MS.gene051689:CDS | 20.0% |
| !!! | ATTTGTTGTGATAATGATTT+TGG | - | chr1.3:4800149-4800168 | MS.gene051689:CDS | 20.0% |
| ! | AAAATGGTTACTACTAATGA+TGG | - | chr1.3:4800462-4800481 | MS.gene051689:CDS | 25.0% |
| ! | AAGAAAAGAGATTATGTTAG+AGG | - | chr1.3:4800273-4800292 | MS.gene051689:CDS | 25.0% |
| ! | AGAAAGATTATGTTAGAAGA+TGG | - | chr1.3:4800430-4800449 | MS.gene051689:CDS | 25.0% |
| ! | GAACAAAAGAAATATCATGA+GGG | + | chr1.3:4799900-4799919 | None:intergenic | 25.0% |
| ! | GAATATGAAGAATATTGAGA+TGG | - | chr1.3:4800104-4800123 | MS.gene051689:CDS | 25.0% |
| ! | GATTTAATATCCTTCAAAGT+TGG | + | chr1.3:4800240-4800259 | None:intergenic | 25.0% |
| !! | CTTTTCTTTTGTATCTTCTA+GGG | + | chr1.3:4799777-4799796 | None:intergenic | 25.0% |
| !! | TAATTTTCACTTGTATTCTG+TGG | - | chr1.3:4800205-4800224 | MS.gene051689:CDS | 25.0% |
| !! | TCTTTTCTTTTGTATCTTCT+AGG | + | chr1.3:4799778-4799797 | None:intergenic | 25.0% |
| !! | TTTTCACCTTAACAACAATA+GGG | + | chr1.3:4799954-4799973 | None:intergenic | 25.0% |
| !! | TTTTTCACCTTAACAACAAT+AGG | + | chr1.3:4799955-4799974 | None:intergenic | 25.0% |
| AACATTCACACAAGAAAAGT+TGG | - | chr1.3:4800547-4800566 | MS.gene051689:CDS | 30.0% | |
| ACAAATGGCAAATAGAACAA+TGG | + | chr1.3:4800135-4800154 | None:intergenic | 30.0% | |
| AGGGTTTAGTGAAAAAAAGA+TGG | - | chr1.3:4800383-4800402 | MS.gene051689:CDS | 30.0% | |
| ATGGATGTAGTGAATAATGA+CGG | - | chr1.3:4800312-4800331 | MS.gene051689:CDS | 30.0% | |
| ATTCCATGAAATTCTCTTCT+TGG | + | chr1.3:4800088-4800107 | None:intergenic | 30.0% | |
| CGAACAAAAGAAATATCATG+AGG | + | chr1.3:4799901-4799920 | None:intergenic | 30.0% | |
| GTATGTTATTCCAACTTTGA+AGG | - | chr1.3:4800227-4800246 | MS.gene051689:CDS | 30.0% | |
| GTTTAGTGAAAAAAAGATGG+TGG | - | chr1.3:4800386-4800405 | MS.gene051689:CDS | 30.0% | |
| TGAATCATCAATTGTTGCTA+TGG | - | chr1.3:4800047-4800066 | MS.gene051689:CDS | 30.0% | |
| TGAATGTTCCACAAACAAAA+GGG | + | chr1.3:4799708-4799727 | None:intergenic | 30.0% | |
| TTCCATGAAATTCTCTTCTT+GGG | + | chr1.3:4800087-4800106 | None:intergenic | 30.0% | |
| TTGAATGTTCCACAAACAAA+AGG | + | chr1.3:4799709-4799728 | None:intergenic | 30.0% | |
| ! | AAAAAGATGGTGGTTAATGA+AGG | - | chr1.3:4800396-4800415 | MS.gene051689:CDS | 30.0% |
| ! | AAAAGATGGTGGTTAATGAA+GGG | - | chr1.3:4800397-4800416 | MS.gene051689:CDS | 30.0% |
| ! | TTTTGCTTGAATCTTGTTCT+TGG | + | chr1.3:4800529-4800548 | None:intergenic | 30.0% |
| !! | AAAGTGCTGAAAATTTATCC+AGG | + | chr1.3:4799831-4799850 | None:intergenic | 30.0% |
| !! | GTTTCACTACTTTTAACTCT+TGG | + | chr1.3:4799984-4800003 | None:intergenic | 30.0% |
| !! | TTTTGTCCCTATTGTTGTTA+AGG | - | chr1.3:4799945-4799964 | MS.gene051689:CDS | 30.0% |
| AACCCAAGAAGAGAATTTCA+TGG | - | chr1.3:4800082-4800101 | MS.gene051689:CDS | 35.0% | |
| AAGATGGAGTGAGAAGAAAA+TGG | - | chr1.3:4800446-4800465 | MS.gene051689:CDS | 35.0% | |
| AGGTTTGAGTGAGAAGAAAA+TGG | - | chr1.3:4800293-4800312 | MS.gene051689:CDS | 35.0% | |
| ATGAAATTCTCTTCTTGGGT+TGG | + | chr1.3:4800083-4800102 | None:intergenic | 35.0% | |
| CATAGATCATCATCATCTTC+TGG | + | chr1.3:4799733-4799752 | None:intergenic | 35.0% | |
| TGGATGTAGTGAATAATGAC+GGG | - | chr1.3:4800313-4800332 | MS.gene051689:CDS | 35.0% | |
| !! | ATGTTGAACCCTTTTGTTTG+TGG | - | chr1.3:4799697-4799716 | MS.gene051689:CDS | 35.0% |
| !! | TATCTTCTAGGGCTTGAATT+AGG | + | chr1.3:4799766-4799785 | None:intergenic | 35.0% |
| !!! | TTGGTTTTGTTGACTGTGTT+TGG | - | chr1.3:4800168-4800187 | MS.gene051689:CDS | 35.0% |
| AAGCTTGAGTCCTAAGTCAA+CGG | - | chr1.3:4800008-4800027 | MS.gene051689:CDS | 40.0% | |
| AATAACCCTTATTCCACACG+TGG | - | chr1.3:4799805-4799824 | MS.gene051689:CDS | 40.0% | |
| GGATGTAGTGAATAATGACG+GGG | - | chr1.3:4800314-4800333 | MS.gene051689:CDS | 40.0% | |
| GGGTGGTGAAGAAAAATGAA+GGG | - | chr1.3:4800334-4800353 | MS.gene051689:CDS | 40.0% | |
| GGTGGTGAAGAAAAATGAAG+GGG | - | chr1.3:4800335-4800354 | MS.gene051689:CDS | 40.0% | |
| TCAAAATCACCACCACTTCT+TGG | + | chr1.3:4800498-4800517 | None:intergenic | 40.0% | |
| TTAGTTTCTCCAAGAAGTGG+TGG | - | chr1.3:4800486-4800505 | MS.gene051689:CDS | 40.0% | |
| !! | GGATTAGTTTCTCCAAGAAG+TGG | - | chr1.3:4800483-4800502 | MS.gene051689:CDS | 40.0% |
| !!! | GACTTTTTCTCCGTTGACTT+AGG | + | chr1.3:4800021-4800040 | None:intergenic | 40.0% |
| AAATTTATCCAGGCCACGTG+TGG | + | chr1.3:4799821-4799840 | None:intergenic | 45.0% | |
| GACTTAGGACTCAAGCTTCT+TGG | + | chr1.3:4800006-4800025 | None:intergenic | 45.0% | |
| GGGGTGGTGAAGAAAAATGA+AGG | - | chr1.3:4800333-4800352 | MS.gene051689:CDS | 45.0% | |
| GGTGAAGAAAAATGAAGGGG+TGG | - | chr1.3:4800338-4800357 | MS.gene051689:CDS | 45.0% | |
| ! | TGTAGTGAATAATGACGGGG+TGG | - | chr1.3:4800317-4800336 | MS.gene051689:CDS | 45.0% |
| CCAGGCCACGTGTGGAATAA+GGG | + | chr1.3:4799813-4799832 | None:intergenic | 55.0% | |
| TCCAGGCCACGTGTGGAATA+AGG | + | chr1.3:4799814-4799833 | None:intergenic | 55.0% | |
| CCCTTATTCCACACGTGGCC+TGG | - | chr1.3:4799810-4799829 | MS.gene051689:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 4799697 | 4800572 | 4799697 | ID=MS.gene051689 |
| chr1.3 | mRNA | 4799697 | 4800572 | 4799697 | ID=MS.gene051689.t1;Parent=MS.gene051689 |
| chr1.3 | exon | 4799697 | 4800572 | 4799697 | ID=MS.gene051689.t1.exon1;Parent=MS.gene051689.t1 |
| chr1.3 | CDS | 4799697 | 4800572 | 4799697 | ID=cds.MS.gene051689.t1;Parent=MS.gene051689.t1 |
| Gene Sequence |
| Protein sequence |
