Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24479.t1 | XP_003589063.1 | 92.6 | 285 | 20 | 1 | 1 | 284 | 1 | 285 | 7.40E-114 | 420.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24479.t1 | G7ICB3 | 92.6 | 285 | 20 | 1 | 1 | 284 | 1 | 285 | 5.4e-114 | 420.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049259 | MS.gene24479 | 0.801249 | 9.84E-49 | -1.69E-46 |
| MS.gene051689 | MS.gene24479 | 0.842839 | 2.02E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24479.t1 | MTR_1g017990 | 93.684 | 285 | 17 | 1 | 1 | 284 | 1 | 285 | 0.0 | 542 |
| MS.gene24479.t1 | MTR_3g103190 | 42.241 | 232 | 97 | 8 | 1 | 207 | 1 | 220 | 1.92e-46 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24479.t1 | AT1G59590 | 39.683 | 189 | 103 | 5 | 22 | 202 | 7 | 192 | 1.14e-32 | 119 |
| MS.gene24479.t1 | AT1G10220 | 40.000 | 180 | 95 | 5 | 22 | 190 | 3 | 180 | 1.48e-25 | 100 |
| MS.gene24479.t1 | AT1G10220 | 37.696 | 191 | 95 | 5 | 22 | 190 | 3 | 191 | 4.82e-24 | 97.4 |
Find 39 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGTTGTGATAATGATTT+TGG | 0.226913 | 1.1:-4647221 | MS.gene24479:CDS |
| TCTAGGCCACGTGTGGAATA+AGG | 0.234724 | 1.1:+4647559 | None:intergenic |
| ATTCCATGAAATTCTCTTCT+TGG | 0.239938 | 1.1:+4647285 | None:intergenic |
| AAGAAAAGAGATTATGTTAG+AGG | 0.255949 | 1.1:-4647097 | MS.gene24479:CDS |
| TATCTGATTTAAATGATAAA+AGG | 0.270978 | 1.1:-4647519 | MS.gene24479:CDS |
| AGGTTTGAGTGAGAAGAAAA+TGG | 0.283239 | 1.1:-4647077 | MS.gene24479:CDS |
| CATAGTTCATCATCATCTTC+TGG | 0.290163 | 1.1:+4647640 | None:intergenic |
| TATCTTCTAGGGCTTGAATT+AGG | 0.299114 | 1.1:+4647607 | None:intergenic |
| TCAAAATCACCACCACTTCT+TGG | 0.326218 | 1.1:+4646896 | None:intergenic |
| GACTTAGGACTCAAGCTTCT+TGG | 0.327238 | 1.1:+4647367 | None:intergenic |
| TTCCATGAAATTCTCTTCTT+GGG | 0.358637 | 1.1:+4647286 | None:intergenic |
| ATGAAATTCTCTTCTTGGGT+TGG | 0.385407 | 1.1:+4647290 | None:intergenic |
| AAGATGGAGTGAGAAGAAAA+TGG | 0.393067 | 1.1:-4646945 | MS.gene24479:CDS |
| TTGAATGTTCCACAAACAAA+AGG | 0.402250 | 1.1:+4647664 | None:intergenic |
| GATTTAATATCCTTCAAAGT+TGG | 0.427450 | 1.1:+4647133 | None:intergenic |
| AAAATCATTATCACAACAAA+TGG | 0.436688 | 1.1:+4647223 | None:intergenic |
| CTAGGCCACGTGTGGAATAA+GGG | 0.436782 | 1.1:+4647560 | None:intergenic |
| AACCCAAGAAGAGAATTTCA+TGG | 0.440286 | 1.1:-4647288 | MS.gene24479:CDS |
| AGAAAGATTATGTTAGAAGA+TGG | 0.449967 | 1.1:-4646961 | MS.gene24479:CDS |
| AACATTCACACAAGAAAAGT+TGG | 0.459312 | 1.1:-4646844 | MS.gene24479:CDS |
| AAAATGGTTACTACTAATGA+TGG | 0.460282 | 1.1:-4646929 | MS.gene24479:CDS |
| ATGGATGTAGTGAATAATGA+AGG | 0.481019 | 1.1:-4647058 | MS.gene24479:CDS |
| AAAGTGCTGAAAATTTATCT+AGG | 0.485667 | 1.1:+4647542 | None:intergenic |
| GTATGTTATTCCAACTTTGA+AGG | 0.503391 | 1.1:-4647143 | MS.gene24479:CDS |
| TGAATCATCAATTGTTGCTA+TGG | 0.523729 | 1.1:-4647323 | MS.gene24479:CDS |
| TGAATGTTCCACAAACAAAA+GGG | 0.542425 | 1.1:+4647665 | None:intergenic |
| GGATTAGTTTCTCCAAGAAG+TGG | 0.582854 | 1.1:-4646908 | MS.gene24479:CDS |
| AAAAGATGGTGGTTAATGAA+GGG | 0.592112 | 1.1:-4646994 | MS.gene24479:CDS |
| GAATATGAAGAATATTGAGA+TGG | 0.602572 | 1.1:-4647266 | MS.gene24479:CDS |
| CGAACAAAAGAAATATCATG+AGG | 0.627048 | 1.1:+4647472 | None:intergenic |
| AAGCTTGAGTCCTAAGTCAA+CGG | 0.627738 | 1.1:-4647362 | MS.gene24479:CDS |
| TTAGTTTCTCCAAGAAGTGG+TGG | 0.628810 | 1.1:-4646905 | MS.gene24479:CDS |
| AAATTTATCTAGGCCACGTG+TGG | 0.632904 | 1.1:+4647552 | None:intergenic |
| ACAAATGGCAAATAGAACAA+TGG | 0.651573 | 1.1:+4647238 | None:intergenic |
| GAACAAAAGAAATATCATGA+GGG | 0.654561 | 1.1:+4647473 | None:intergenic |
| TGGATGTAGTGAATAATGAA+GGG | 0.664223 | 1.1:-4647057 | MS.gene24479:CDS |
| TGTAGTGAATAATGAAGGGG+TGG | 0.690570 | 1.1:-4647053 | MS.gene24479:CDS |
| AATAACCCTTATTCCACACG+TGG | 0.697457 | 1.1:-4647565 | MS.gene24479:CDS |
| GGATGTAGTGAATAATGAAG+GGG | 0.711044 | 1.1:-4647056 | MS.gene24479:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATCTGATTTAAATGATAAA+AGG | - | chr1.1:4646995-4647014 | MS.gene24479:CDS | 15.0% |
| !! | AAAATCATTATCACAACAAA+TGG | + | chr1.1:4647294-4647313 | None:intergenic | 20.0% |
| !! | AAGAAAAAAGATTATGTTAG+AGG | - | chr1.1:4647486-4647505 | MS.gene24479:CDS | 20.0% |
| !! | AGAAAAAAGATTATGTTAGA+GGG | - | chr1.1:4647487-4647506 | MS.gene24479:CDS | 20.0% |
| !!! | ATTTGTTGTGATAATGATTT+TGG | - | chr1.1:4647293-4647312 | MS.gene24479:CDS | 20.0% |
| ! | AAAATGGTTACTACTAATGA+TGG | - | chr1.1:4647585-4647604 | MS.gene24479:CDS | 25.0% |
| ! | AAGAAAAGAGATTATGTTAG+AGG | - | chr1.1:4647417-4647436 | MS.gene24479:CDS | 25.0% |
| ! | AGAAAGATTATGTTAGAAGA+TGG | - | chr1.1:4647553-4647572 | MS.gene24479:CDS | 25.0% |
| ! | GAACAAAAGAAATATCATGA+GGG | + | chr1.1:4647044-4647063 | None:intergenic | 25.0% |
| ! | GAATATGAAGAATATTGAGA+TGG | - | chr1.1:4647248-4647267 | MS.gene24479:CDS | 25.0% |
| ! | GATTTAATATCCTTCAAAGT+TGG | + | chr1.1:4647384-4647403 | None:intergenic | 25.0% |
| !! | CTTTTCTTTTGTATCTTCTA+GGG | + | chr1.1:4646921-4646940 | None:intergenic | 25.0% |
| !! | TAATTTTCACTTGTATTCTG+TGG | - | chr1.1:4647349-4647368 | MS.gene24479:CDS | 25.0% |
| !! | TCTTTTCTTTTGTATCTTCT+AGG | + | chr1.1:4646922-4646941 | None:intergenic | 25.0% |
| !! | TTTTCACCTTAACAACAATA+GGG | + | chr1.1:4647098-4647117 | None:intergenic | 25.0% |
| !! | TTTTTCACCTTAACAACAAT+AGG | + | chr1.1:4647099-4647118 | None:intergenic | 25.0% |
| !!! | AAAGTGCTGAAAATTTATCT+AGG | + | chr1.1:4646975-4646994 | None:intergenic | 25.0% |
| AACATTCACACAAGAAAAGT+TGG | - | chr1.1:4647670-4647689 | MS.gene24479:CDS | 30.0% | |
| ACAAATGGCAAATAGAACAA+TGG | + | chr1.1:4647279-4647298 | None:intergenic | 30.0% | |
| AGGGTTTAGTGAAAAAAAGA+TGG | - | chr1.1:4647506-4647525 | MS.gene24479:CDS | 30.0% | |
| ATGGATGTAGTGAATAATGA+AGG | - | chr1.1:4647456-4647475 | MS.gene24479:CDS | 30.0% | |
| ATTCCATGAAATTCTCTTCT+TGG | + | chr1.1:4647232-4647251 | None:intergenic | 30.0% | |
| CGAACAAAAGAAATATCATG+AGG | + | chr1.1:4647045-4647064 | None:intergenic | 30.0% | |
| GTATGTTATTCCAACTTTGA+AGG | - | chr1.1:4647371-4647390 | MS.gene24479:CDS | 30.0% | |
| GTTTAGTGAAAAAAAGATGG+TGG | - | chr1.1:4647509-4647528 | MS.gene24479:CDS | 30.0% | |
| TGAATCATCAATTGTTGCTA+TGG | - | chr1.1:4647191-4647210 | MS.gene24479:CDS | 30.0% | |
| TGAATGTTCCACAAACAAAA+GGG | + | chr1.1:4646852-4646871 | None:intergenic | 30.0% | |
| TGGATGTAGTGAATAATGAA+GGG | - | chr1.1:4647457-4647476 | MS.gene24479:CDS | 30.0% | |
| TTCCATGAAATTCTCTTCTT+GGG | + | chr1.1:4647231-4647250 | None:intergenic | 30.0% | |
| TTGAATGTTCCACAAACAAA+AGG | + | chr1.1:4646853-4646872 | None:intergenic | 30.0% | |
| ! | AAAAAGATGGTGGTTAATGA+AGG | - | chr1.1:4647519-4647538 | MS.gene24479:CDS | 30.0% |
| ! | AAAAGATGGTGGTTAATGAA+GGG | - | chr1.1:4647520-4647539 | MS.gene24479:CDS | 30.0% |
| ! | TTTTGCTTGAATCTTGTTCT+TGG | + | chr1.1:4647652-4647671 | None:intergenic | 30.0% |
| !! | GTTTCACTACTTTTAACTCT+TGG | + | chr1.1:4647128-4647147 | None:intergenic | 30.0% |
| !! | TTTTGTCCCTATTGTTGTTA+AGG | - | chr1.1:4647089-4647108 | MS.gene24479:CDS | 30.0% |
| AACCCAAGAAGAGAATTTCA+TGG | - | chr1.1:4647226-4647245 | MS.gene24479:CDS | 35.0% | |
| AAGATGGAGTGAGAAGAAAA+TGG | - | chr1.1:4647569-4647588 | MS.gene24479:CDS | 35.0% | |
| AGGTTTGAGTGAGAAGAAAA+TGG | - | chr1.1:4647437-4647456 | MS.gene24479:CDS | 35.0% | |
| ATGAAATTCTCTTCTTGGGT+TGG | + | chr1.1:4647227-4647246 | None:intergenic | 35.0% | |
| CATAGTTCATCATCATCTTC+TGG | + | chr1.1:4646877-4646896 | None:intergenic | 35.0% | |
| GGATGTAGTGAATAATGAAG+GGG | - | chr1.1:4647458-4647477 | MS.gene24479:CDS | 35.0% | |
| !! | ATGTTGAACCCTTTTGTTTG+TGG | - | chr1.1:4646841-4646860 | MS.gene24479:CDS | 35.0% |
| !! | TATCTTCTAGGGCTTGAATT+AGG | + | chr1.1:4646910-4646929 | None:intergenic | 35.0% |
| !!! | TTGGTTTTGTTGACTGTGTT+TGG | - | chr1.1:4647312-4647331 | MS.gene24479:CDS | 35.0% |
| AAATTTATCTAGGCCACGTG+TGG | + | chr1.1:4646965-4646984 | None:intergenic | 40.0% | |
| AAGCTTGAGTCCTAAGTCAA+CGG | - | chr1.1:4647152-4647171 | MS.gene24479:CDS | 40.0% | |
| AATAACCCTTATTCCACACG+TGG | - | chr1.1:4646949-4646968 | MS.gene24479:CDS | 40.0% | |
| TCAAAATCACCACCACTTCT+TGG | + | chr1.1:4647621-4647640 | None:intergenic | 40.0% | |
| TGTAGTGAATAATGAAGGGG+TGG | - | chr1.1:4647461-4647480 | MS.gene24479:CDS | 40.0% | |
| TTAGTTTCTCCAAGAAGTGG+TGG | - | chr1.1:4647609-4647628 | MS.gene24479:CDS | 40.0% | |
| !! | GGATTAGTTTCTCCAAGAAG+TGG | - | chr1.1:4647606-4647625 | MS.gene24479:CDS | 40.0% |
| !!! | GACTTTTTCTCCGTTGACTT+AGG | + | chr1.1:4647165-4647184 | None:intergenic | 40.0% |
| GACTTAGGACTCAAGCTTCT+TGG | + | chr1.1:4647150-4647169 | None:intergenic | 45.0% | |
| CTAGGCCACGTGTGGAATAA+GGG | + | chr1.1:4646957-4646976 | None:intergenic | 50.0% | |
| TCTAGGCCACGTGTGGAATA+AGG | + | chr1.1:4646958-4646977 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 4646841 | 4647695 | 4646841 | ID=MS.gene24479 |
| chr1.1 | mRNA | 4646841 | 4647695 | 4646841 | ID=MS.gene24479.t1;Parent=MS.gene24479 |
| chr1.1 | exon | 4646841 | 4647695 | 4646841 | ID=MS.gene24479.t1.exon1;Parent=MS.gene24479.t1 |
| chr1.1 | CDS | 4646841 | 4647695 | 4646841 | ID=cds.MS.gene24479.t1;Parent=MS.gene24479.t1 |
| Gene Sequence |
| Protein sequence |
