Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051691.t1 | XP_003589065.1 | 97.4 | 77 | 2 | 0 | 1 | 77 | 10 | 86 | 4.20E-19 | 103.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051691.t1 | G7ICB5 | 97.4 | 77 | 2 | 0 | 1 | 77 | 10 | 86 | 3.0e-19 | 103.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051691 | MS.gene66461 | 0.845388 | 4.19E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051691.t1 | MTR_1g018020 | 97.403 | 77 | 2 | 0 | 1 | 77 | 10 | 86 | 3.66e-49 | 150 |
| MS.gene051691.t1 | MTR_3g103180 | 63.014 | 73 | 18 | 2 | 1 | 73 | 1 | 64 | 3.51e-13 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051691.t1 | AT5G63905 | 69.863 | 73 | 22 | 0 | 4 | 76 | 3 | 75 | 5.18e-13 | 58.5 |
Find 17 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCAATCGTTCTTCTTCTT+TGG | 0.267606 | 1.3:+4780795 | None:intergenic |
| GCTGCACGTGCACATCAAAA+AGG | 0.391519 | 1.3:-4780114 | MS.gene051691:CDS |
| AAGAAGATGGGTATATGCTT+TGG | 0.393509 | 1.3:-4780847 | None:intergenic |
| CAAGCTGCAAACTCAAATAA+AGG | 0.408424 | 1.3:-4780081 | MS.gene051691:CDS |
| CTGCTGAAGCCGCTCAGAAA+AGG | 0.423944 | 1.3:-4780750 | MS.gene051691:intron |
| GGTATATGCTTTGGTTGCTT+CGG | 0.447661 | 1.3:-4780838 | MS.gene051691:CDS |
| TCTGTGGATGCAGTGGCAGA+TGG | 0.491765 | 1.3:-4779871 | MS.gene051691:intron |
| ATATGCTTTGGTTGCTTCGG+TGG | 0.510299 | 1.3:-4780835 | MS.gene051691:CDS |
| TGCTTTGGTTGCTTCGGTGG+CGG | 0.510377 | 1.3:-4780832 | MS.gene051691:CDS |
| TAAAGGCGAACCAACTCTAA+AGG | 0.521986 | 1.3:-4780064 | MS.gene051691:intron |
| GACTTTGAGAAATCTGCAGC+AGG | 0.532357 | 1.3:-4780141 | MS.gene051691:CDS |
| GACCAAAGAAGAAGAACGAT+TGG | 0.539638 | 1.3:-4780797 | MS.gene051691:CDS |
| AGTCACCAAACCTTTAGAGT+TGG | 0.547268 | 1.3:+4780054 | None:intergenic |
| CTGTGGATGCAGTGGCAGAT+GGG | 0.552408 | 1.3:-4779870 | MS.gene051691:intron |
| TCTAGCACGCGCTTCTTCAG+AGG | 0.566908 | 1.3:+4780774 | None:intergenic |
| ATGCAGTGGCAGATGGGCTG+AGG | 0.609439 | 1.3:-4779864 | None:intergenic |
| TTGTGTTGATTGCAGGCAAG+AGG | 0.616418 | 1.3:-4780163 | MS.gene051691:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAAAAAAAAACTTTGT+AGG | + | chr1.3:4780055-4780074 | None:intergenic | 10.0% |
| !! | TTAAATTCATTACATATTTA+GGG | + | chr1.3:4780397-4780416 | None:intergenic | 10.0% |
| !!! | CAAAGTTTTTTTTTTTTTAA+CGG | - | chr1.3:4780056-4780075 | MS.gene051691:intron | 10.0% |
| !!! | TTGTATTAAAAAATGAATTT+TGG | - | chr1.3:4780149-4780168 | MS.gene051691:CDS | 10.0% |
| !! | AAAATAAATGATAACAAAAG+TGG | + | chr1.3:4780708-4780727 | None:intergenic | 15.0% |
| !! | CTTAAATTCATTACATATTT+AGG | + | chr1.3:4780398-4780417 | None:intergenic | 15.0% |
| !!! | TTTTTTTTTTTAACGGATTT+GGG | - | chr1.3:4780063-4780082 | MS.gene051691:intron | 15.0% |
| !!! | TTTTTTTTTTTTAACGGATT+TGG | - | chr1.3:4780062-4780081 | MS.gene051691:intron | 15.0% |
| !! | TAAATGTATCTGAAAATTGT+TGG | + | chr1.3:4780784-4780803 | None:intergenic | 20.0% |
| !! | TTTCATTTCAATACTCATAT+GGG | - | chr1.3:4780088-4780107 | MS.gene051691:CDS | 20.0% |
| !!! | TTTTCATTTCAATACTCATA+TGG | - | chr1.3:4780087-4780106 | MS.gene051691:CDS | 20.0% |
| ! | AAATTGTTGGTCAAAAAACA+TGG | + | chr1.3:4780771-4780790 | None:intergenic | 25.0% |
| ! | AATTGTACAATTAAGTTTGG+TGG | + | chr1.3:4780032-4780051 | None:intergenic | 25.0% |
| ! | AATTGTTGGTCAAAAAACAT+GGG | + | chr1.3:4780770-4780789 | None:intergenic | 25.0% |
| ! | AGGAATTGTACAATTAAGTT+TGG | + | chr1.3:4780035-4780054 | None:intergenic | 25.0% |
| ! | ATAGAAACAACTTATGAGAT+GGG | + | chr1.3:4780305-4780324 | None:intergenic | 25.0% |
| ! | ATTCAGGAGAAAAAAGAAAA+GGG | + | chr1.3:4780006-4780025 | None:intergenic | 25.0% |
| ! | TATAGAAACAACTTATGAGA+TGG | + | chr1.3:4780306-4780325 | None:intergenic | 25.0% |
| ! | TATATTCTTTGTGATCATAC+TGG | - | chr1.3:4780115-4780134 | MS.gene051691:CDS | 25.0% |
| ! | TATTCAGGAGAAAAAAGAAA+AGG | + | chr1.3:4780007-4780026 | None:intergenic | 25.0% |
| !!! | ATGTTATGATGTTATTTTGC+TGG | - | chr1.3:4780470-4780489 | MS.gene051691:intron | 25.0% |
| !!! | CATTTTGATTCTTTTATCTG+TGG | - | chr1.3:4780820-4780839 | MS.gene051691:CDS | 25.0% |
| AAACACTTGTGAAACTGTTT+GGG | + | chr1.3:4780335-4780354 | None:intergenic | 30.0% | |
| AAAGGGGAATTTAGAGAAAA+AGG | + | chr1.3:4779989-4780008 | None:intergenic | 30.0% | |
| ACTAGAGATAAGCTCAAATA+AGG | - | chr1.3:4780266-4780285 | MS.gene051691:intron | 30.0% | |
| CTCAAATAAGGCAATTCAAA+CGG | - | chr1.3:4780278-4780297 | MS.gene051691:intron | 30.0% | |
| GAATTTAAGCTTACATGTTG+AGG | - | chr1.3:4780409-4780428 | MS.gene051691:intron | 30.0% | |
| GTAGAAACTCTTAATAAAGG+TGG | + | chr1.3:4780686-4780705 | None:intergenic | 30.0% | |
| GTGGTAGAAACTCTTAATAA+AGG | + | chr1.3:4780689-4780708 | None:intergenic | 30.0% | |
| TAAACACTTGTGAAACTGTT+TGG | + | chr1.3:4780336-4780355 | None:intergenic | 30.0% | |
| TCAAATAAGGCAATTCAAAC+GGG | - | chr1.3:4780279-4780298 | MS.gene051691:intron | 30.0% | |
| TTCAGGAGAAAAAAGAAAAG+GGG | + | chr1.3:4780005-4780024 | None:intergenic | 30.0% | |
| ! | TTAAGTTTGGTGGAATATTC+AGG | + | chr1.3:4780022-4780041 | None:intergenic | 30.0% |
| ! | TTACATATTTAGGGTGTGTT+TGG | + | chr1.3:4780388-4780407 | None:intergenic | 30.0% |
| AAAGAAAGCAGCATATTGCA+AGG | + | chr1.3:4780743-4780762 | None:intergenic | 35.0% | |
| CAAGCTGCAAACTCAAATAA+AGG | - | chr1.3:4780626-4780645 | MS.gene051691:intron | 35.0% | |
| TTAAGCTTACATGTTGAGGT+GGG | - | chr1.3:4780413-4780432 | MS.gene051691:intron | 35.0% | |
| TTTAAGCTTACATGTTGAGG+TGG | - | chr1.3:4780412-4780431 | MS.gene051691:intron | 35.0% | |
| ! | AGGTTTAAACCTTTTCTGAG+CGG | + | chr1.3:4779969-4779988 | None:intergenic | 35.0% |
| ACGTGCACATCAAAAAGGAA+TGG | - | chr1.3:4780598-4780617 | MS.gene051691:intron | 40.0% | |
| GACCAAAGAAGAAGAACGAT+TGG | - | chr1.3:4779910-4779929 | MS.gene051691:intron | 40.0% | |
| GGTATATGCTTTGGTTGCTT+CGG | - | chr1.3:4779869-4779888 | MS.gene051691:intron | 40.0% | |
| TAAAGGCGAACCAACTCTAA+AGG | - | chr1.3:4780643-4780662 | MS.gene051691:intron | 40.0% | |
| TAAGCTTACATGTTGAGGTG+GGG | - | chr1.3:4780414-4780433 | MS.gene051691:intron | 40.0% | |
| ! | TCTTTTATCTGTGGATGCAG+TGG | - | chr1.3:4780829-4780848 | MS.gene051691:CDS | 40.0% |
| !! | AGTCACCAAACCTTTAGAGT+TGG | + | chr1.3:4780656-4780675 | None:intergenic | 40.0% |
| !! | GTATGTGTTGTGTTGATTGC+AGG | - | chr1.3:4780537-4780556 | MS.gene051691:intron | 40.0% |
| AAGCTTACATGTTGAGGTGG+GGG | - | chr1.3:4780415-4780434 | MS.gene051691:intron | 45.0% | |
| ATATGCTTTGGTTGCTTCGG+TGG | - | chr1.3:4779872-4779891 | MS.gene051691:intron | 45.0% | |
| GGCCAATCGTTCTTCTTCTT+TGG | + | chr1.3:4779915-4779934 | None:intergenic | 45.0% | |
| ! | GACTTTGAGAAATCTGCAGC+AGG | - | chr1.3:4780566-4780585 | MS.gene051691:intron | 45.0% |
| ! | GCGAACCAACTCTAAAGGTT+TGG | - | chr1.3:4780648-4780667 | MS.gene051691:intron | 45.0% |
| !! | TTGTGTTGATTGCAGGCAAG+AGG | - | chr1.3:4780544-4780563 | MS.gene051691:intron | 45.0% |
| GCTGCACGTGCACATCAAAA+AGG | - | chr1.3:4780593-4780612 | MS.gene051691:intron | 50.0% | |
| CTGCTGAAGCCGCTCAGAAA+AGG | - | chr1.3:4779957-4779976 | MS.gene051691:intron | 55.0% | |
| CTGTGGATGCAGTGGCAGAT+GGG | - | chr1.3:4780837-4780856 | MS.gene051691:CDS | 55.0% | |
| CTTACATGTTGAGGTGGGGG+TGG | - | chr1.3:4780418-4780437 | MS.gene051691:intron | 55.0% | |
| TCTAGCACGCGCTTCTTCAG+AGG | + | chr1.3:4779936-4779955 | None:intergenic | 55.0% | |
| TCTGTGGATGCAGTGGCAGA+TGG | - | chr1.3:4780836-4780855 | MS.gene051691:CDS | 55.0% | |
| TGCTTTGGTTGCTTCGGTGG+CGG | - | chr1.3:4779875-4779894 | MS.gene051691:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 4779866 | 4780863 | 4779866 | ID=MS.gene051691 |
| chr1.3 | mRNA | 4779866 | 4780863 | 4779866 | ID=MS.gene051691.t1;Parent=MS.gene051691 |
| chr1.3 | exon | 4780751 | 4780863 | 4780751 | ID=MS.gene051691.t1.exon1;Parent=MS.gene051691.t1 |
| chr1.3 | CDS | 4780751 | 4780863 | 4780751 | ID=cds.MS.gene051691.t1;Parent=MS.gene051691.t1 |
| chr1.3 | exon | 4780065 | 4780170 | 4780065 | ID=MS.gene051691.t1.exon2;Parent=MS.gene051691.t1 |
| chr1.3 | CDS | 4780065 | 4780170 | 4780065 | ID=cds.MS.gene051691.t1;Parent=MS.gene051691.t1 |
| chr1.3 | exon | 4779866 | 4779880 | 4779866 | ID=MS.gene051691.t1.exon3;Parent=MS.gene051691.t1 |
| chr1.3 | CDS | 4779866 | 4779880 | 4779866 | ID=cds.MS.gene051691.t1;Parent=MS.gene051691.t1 |
| Gene Sequence |
| Protein sequence |
