Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66461.t1 | XP_003589065.1 | 94.2 | 86 | 3 | 1 | 1 | 84 | 1 | 86 | 3.70E-21 | 110.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66461.t1 | G7ICB5 | 94.2 | 86 | 3 | 1 | 1 | 84 | 1 | 86 | 2.7e-21 | 110.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05029 | MS.gene66461 | 0.814089 | 1.84E-51 | -1.69E-46 |
| MS.gene051691 | MS.gene66461 | 0.845388 | 4.19E-59 | -1.69E-46 |
| MS.gene057130 | MS.gene66461 | 0.831386 | 1.72E-55 | -1.69E-46 |
| MS.gene06118 | MS.gene66461 | 0.81956 | 1.09E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66461.t1 | MTR_1g018020 | 94.186 | 86 | 3 | 1 | 1 | 84 | 1 | 86 | 1.76e-30 | 103 |
| MS.gene66461.t1 | MTR_3g103180 | 63.014 | 73 | 18 | 2 | 8 | 80 | 1 | 64 | 5.96e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66461.t1 | AT5G63905 | 69.863 | 73 | 22 | 0 | 11 | 83 | 3 | 75 | 6.62e-13 | 58.5 |
Find 19 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCAATCGTTCTTCTTCTT+TGG | 0.267606 | 1.4:+4951427 | None:intergenic |
| GCTGCACGTGCACATCAAAA+AGG | 0.391519 | 1.4:-4950740 | MS.gene66461:CDS |
| AAGAAGATGGGTATATGCTT+TGG | 0.393509 | 1.4:-4951479 | MS.gene66461:CDS |
| CAAGCTGCAAACTCAAATAA+AGG | 0.408424 | 1.4:-4950707 | MS.gene66461:CDS |
| CTGCTGAAGCCGCTCAGAAA+AGG | 0.423944 | 1.4:-4951382 | MS.gene66461:intron |
| GGTATATGCTTTGGTTGCTT+CGG | 0.447661 | 1.4:-4951470 | MS.gene66461:CDS |
| GAACGAAGGATTAAAGAAGA+TGG | 0.473381 | 1.4:-4951492 | MS.gene66461:CDS |
| TCTGTGGATGCAGTGGCAGA+TGG | 0.491765 | 1.4:-4950497 | MS.gene66461:intron |
| AACGAAGGATTAAAGAAGAT+GGG | 0.507717 | 1.4:-4951491 | MS.gene66461:CDS |
| ATATGCTTTGGTTGCTTCGG+TGG | 0.510299 | 1.4:-4951467 | MS.gene66461:CDS |
| TGCTTTGGTTGCTTCGGTGG+CGG | 0.510377 | 1.4:-4951464 | MS.gene66461:CDS |
| TAAAGGCGAACCAACTCTAA+AGG | 0.521986 | 1.4:-4950690 | MS.gene66461:intron |
| GACTTTGAGAAATCTGCAGC+AGG | 0.532357 | 1.4:-4950767 | MS.gene66461:CDS |
| GACCAAAGAAGAAGAACGAT+TGG | 0.539638 | 1.4:-4951429 | MS.gene66461:CDS |
| AGTCACCAAACCTTTAGAGT+TGG | 0.547268 | 1.4:+4950680 | None:intergenic |
| CTGTGGATGCAGTGGCAGAT+GGG | 0.552408 | 1.4:-4950496 | MS.gene66461:intron |
| TCTAGCACGCGCTTCTTCAG+AGG | 0.566908 | 1.4:+4951406 | None:intergenic |
| ATGCAGTGGCAGATGGGCTG+AGG | 0.609439 | 1.4:-4950490 | None:intergenic |
| TTGTGTTGATTGCAGGCAAG+AGG | 0.616418 | 1.4:-4950789 | MS.gene66461:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTTTTTTTAA+CGG | - | chr1.4:4950709-4950728 | MS.gene66461:CDS | 0.0% |
| !! | AAAAAAAAAAAAAACTTTGT+AGG | + | chr1.4:4950702-4950721 | None:intergenic | 10.0% |
| !! | TTAAATTCATTACATATTTA+GGG | + | chr1.4:4951050-4951069 | None:intergenic | 10.0% |
| !!! | TTGTATTAAAAAATGAATTT+TGG | - | chr1.4:4950802-4950821 | MS.gene66461:intron | 10.0% |
| !! | AAAATAAATGATAACAAAAG+TGG | + | chr1.4:4951361-4951380 | None:intergenic | 15.0% |
| !! | CTTAAATTCATTACATATTT+AGG | + | chr1.4:4951051-4951070 | None:intergenic | 15.0% |
| !!! | TTTTTTTTTTTAACGGATTT+GGG | - | chr1.4:4950716-4950735 | MS.gene66461:CDS | 15.0% |
| !!! | TTTTTTTTTTTTAACGGATT+TGG | - | chr1.4:4950715-4950734 | MS.gene66461:CDS | 15.0% |
| !! | TAAATGTATCTGAAAATTGT+TGG | + | chr1.4:4951437-4951456 | None:intergenic | 20.0% |
| !! | TTTCATTTCAATACTCATAT+GGG | - | chr1.4:4950741-4950760 | MS.gene66461:CDS | 20.0% |
| !!! | TTTTCATTTCAATACTCATA+TGG | - | chr1.4:4950740-4950759 | MS.gene66461:CDS | 20.0% |
| ! | AAATTGTTGGTCAAAAAACA+TGG | + | chr1.4:4951424-4951443 | None:intergenic | 25.0% |
| ! | AATTGTACAATTAAGTTTGG+TGG | + | chr1.4:4950679-4950698 | None:intergenic | 25.0% |
| ! | AATTGTTGGTCAAAAAACAT+GGG | + | chr1.4:4951423-4951442 | None:intergenic | 25.0% |
| ! | AGGAATTGTACAATTAAGTT+TGG | + | chr1.4:4950682-4950701 | None:intergenic | 25.0% |
| ! | ATAGAAACAACTTATGAGAT+GGG | + | chr1.4:4950958-4950977 | None:intergenic | 25.0% |
| ! | ATTCAGGAGAAAAAAGAAAA+GGG | + | chr1.4:4950653-4950672 | None:intergenic | 25.0% |
| ! | TATAGAAACAACTTATGAGA+TGG | + | chr1.4:4950959-4950978 | None:intergenic | 25.0% |
| ! | TATATTCTTTGTGATCATAC+TGG | - | chr1.4:4950768-4950787 | MS.gene66461:CDS | 25.0% |
| ! | TATTCAGGAGAAAAAAGAAA+AGG | + | chr1.4:4950654-4950673 | None:intergenic | 25.0% |
| !!! | ATGTTATGATGTTATTTTGC+TGG | - | chr1.4:4951123-4951142 | MS.gene66461:intron | 25.0% |
| AAACACTTGTGAAACTGTTT+GGG | + | chr1.4:4950988-4951007 | None:intergenic | 30.0% | |
| AAAGGGGAATTTAGAGAAAA+AGG | + | chr1.4:4950636-4950655 | None:intergenic | 30.0% | |
| AACGAAGGATTAAAGAAGAT+GGG | - | chr1.4:4950495-4950514 | MS.gene66461:intron | 30.0% | |
| ACTAGAGATAAGCTCAAATA+AGG | - | chr1.4:4950919-4950938 | MS.gene66461:intron | 30.0% | |
| CTCAAATAAGGCAATTCAAA+CGG | - | chr1.4:4950931-4950950 | MS.gene66461:intron | 30.0% | |
| GAATTTAAGCTTACATGTTG+AGG | - | chr1.4:4951062-4951081 | MS.gene66461:intron | 30.0% | |
| GTAGAAACTCTTAATAAAGG+TGG | + | chr1.4:4951339-4951358 | None:intergenic | 30.0% | |
| GTGGTAGAAACTCTTAATAA+AGG | + | chr1.4:4951342-4951361 | None:intergenic | 30.0% | |
| TAAACACTTGTGAAACTGTT+TGG | + | chr1.4:4950989-4951008 | None:intergenic | 30.0% | |
| TCAAATAAGGCAATTCAAAC+GGG | - | chr1.4:4950932-4950951 | MS.gene66461:intron | 30.0% | |
| TTCAGGAGAAAAAAGAAAAG+GGG | + | chr1.4:4950652-4950671 | None:intergenic | 30.0% | |
| ! | TTAAGTTTGGTGGAATATTC+AGG | + | chr1.4:4950669-4950688 | None:intergenic | 30.0% |
| ! | TTACATATTTAGGGTGTGTT+TGG | + | chr1.4:4951041-4951060 | None:intergenic | 30.0% |
| !!! | CGTTTTGATTCTTTTATCTG+TGG | - | chr1.4:4951473-4951492 | MS.gene66461:CDS | 30.0% |
| AAAGAAAGCAGCATATTGCA+AGG | + | chr1.4:4951396-4951415 | None:intergenic | 35.0% | |
| AAGAAGATGGGTATATGCTT+TGG | - | chr1.4:4950507-4950526 | MS.gene66461:intron | 35.0% | |
| CAAGCTGCAAACTCAAATAA+AGG | - | chr1.4:4951279-4951298 | MS.gene66461:intron | 35.0% | |
| GAACGAAGGATTAAAGAAGA+TGG | - | chr1.4:4950494-4950513 | MS.gene66461:intron | 35.0% | |
| TTAAGCTTACATGTTGAGGT+GGG | - | chr1.4:4951066-4951085 | MS.gene66461:intron | 35.0% | |
| TTTAAGCTTACATGTTGAGG+TGG | - | chr1.4:4951065-4951084 | MS.gene66461:intron | 35.0% | |
| ! | AGGTTTAAACCTTTTCTGAG+CGG | + | chr1.4:4950616-4950635 | None:intergenic | 35.0% |
| ACGTGCACATCAAAAAGGAA+TGG | - | chr1.4:4951251-4951270 | MS.gene66461:intron | 40.0% | |
| GACCAAAGAAGAAGAACGAT+TGG | - | chr1.4:4950557-4950576 | MS.gene66461:intron | 40.0% | |
| GGTATATGCTTTGGTTGCTT+CGG | - | chr1.4:4950516-4950535 | MS.gene66461:intron | 40.0% | |
| TAAAGGCGAACCAACTCTAA+AGG | - | chr1.4:4951296-4951315 | MS.gene66461:intron | 40.0% | |
| TAAGCTTACATGTTGAGGTG+GGG | - | chr1.4:4951067-4951086 | MS.gene66461:intron | 40.0% | |
| ! | TCTTTTATCTGTGGATGCAG+TGG | - | chr1.4:4951482-4951501 | MS.gene66461:CDS | 40.0% |
| !! | AGTCACCAAACCTTTAGAGT+TGG | + | chr1.4:4951309-4951328 | None:intergenic | 40.0% |
| !! | GTATGTGTTGTGTTGATTGC+AGG | - | chr1.4:4951190-4951209 | MS.gene66461:intron | 40.0% |
| AAGCTTACATGTTGAGGTGG+GGG | - | chr1.4:4951068-4951087 | MS.gene66461:intron | 45.0% | |
| ATATGCTTTGGTTGCTTCGG+TGG | - | chr1.4:4950519-4950538 | MS.gene66461:intron | 45.0% | |
| GGCCAATCGTTCTTCTTCTT+TGG | + | chr1.4:4950562-4950581 | None:intergenic | 45.0% | |
| ! | GACTTTGAGAAATCTGCAGC+AGG | - | chr1.4:4951219-4951238 | MS.gene66461:intron | 45.0% |
| ! | GCGAACCAACTCTAAAGGTT+TGG | - | chr1.4:4951301-4951320 | MS.gene66461:intron | 45.0% |
| !! | TTGTGTTGATTGCAGGCAAG+AGG | - | chr1.4:4951197-4951216 | MS.gene66461:intron | 45.0% |
| GCTGCACGTGCACATCAAAA+AGG | - | chr1.4:4951246-4951265 | MS.gene66461:intron | 50.0% | |
| CTGCTGAAGCCGCTCAGAAA+AGG | - | chr1.4:4950604-4950623 | MS.gene66461:intron | 55.0% | |
| CTGTGGATGCAGTGGCAGAT+GGG | - | chr1.4:4951490-4951509 | MS.gene66461:CDS | 55.0% | |
| CTTACATGTTGAGGTGGGGG+TGG | - | chr1.4:4951071-4951090 | MS.gene66461:intron | 55.0% | |
| TCTAGCACGCGCTTCTTCAG+AGG | + | chr1.4:4950583-4950602 | None:intergenic | 55.0% | |
| TCTGTGGATGCAGTGGCAGA+TGG | - | chr1.4:4951489-4951508 | MS.gene66461:CDS | 55.0% | |
| TGCTTTGGTTGCTTCGGTGG+CGG | - | chr1.4:4950522-4950541 | MS.gene66461:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 4950492 | 4951516 | 4950492 | ID=MS.gene66461 |
| chr1.4 | mRNA | 4950492 | 4951516 | 4950492 | ID=MS.gene66461.t1;Parent=MS.gene66461 |
| chr1.4 | exon | 4951383 | 4951516 | 4951383 | ID=MS.gene66461.t1.exon1;Parent=MS.gene66461.t1 |
| chr1.4 | CDS | 4951383 | 4951516 | 4951383 | ID=cds.MS.gene66461.t1;Parent=MS.gene66461.t1 |
| chr1.4 | exon | 4950691 | 4950796 | 4950691 | ID=MS.gene66461.t1.exon2;Parent=MS.gene66461.t1 |
| chr1.4 | CDS | 4950691 | 4950796 | 4950691 | ID=cds.MS.gene66461.t1;Parent=MS.gene66461.t1 |
| chr1.4 | exon | 4950492 | 4950506 | 4950492 | ID=MS.gene66461.t1.exon3;Parent=MS.gene66461.t1 |
| chr1.4 | CDS | 4950492 | 4950506 | 4950492 | ID=cds.MS.gene66461.t1;Parent=MS.gene66461.t1 |
| Gene Sequence |
| Protein sequence |
