AlfalfaGEDB | MS.gene051755

M. sativa cultivar XinJiangDaYe / MS.gene051755

KEGG: K00419
GO: GO:0005575 GO:0005743 GO:0005750 GO:0006091 GO:0006122 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0019866 GO:0022900 GO:0022904 GO:0031090 GO:0031966 GO:0032991 GO:0044237 GO:0045275 GO:0055114 GO:0070069 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0110165 GO:1902494 GO:1990204
PFAM: PF05365.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051755.t1	XP_003625817.1	100	56	0	0	1	56	1	56	6.00E-23	115.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051755.t1	P46270	78.6	56	12	0	1	56	1	56	1.7e-18	92.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051755.t1	G7L638	100.0	56	0	0	1	56	1	56	4.3e-23	115.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051755	MS.gene051756	0.871499	6.52E-67	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051755.t1	MTR_7g104570	100.000	56	0	0	1	56	1	56	3.61e-35	113

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051755.t1	AT3G52730	76.786	56	13	0	1	56	1	56	8.88e-27	92.4
MS.gene051755.t1	AT3G52730	76.786	56	13	0	1	56	1	56	1.10e-26	92.0

Find 15 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAGAGGAGGAGGCGTTT+TGG	0.335531	7.4:-7633711	MS.gene051755:CDS
TCTGGGAAATAAATAATGTT+GGG	0.387527	7.4:-7633501	MS.gene051755:CDS
TCGTTGCAGGCTGTGGATTA+CGG	0.406296	7.4:-7633535	MS.gene051755:intron
ATTACGGTGTTCACAAGCTC+TGG	0.424543	7.4:-7633519	MS.gene051755:CDS
CTCTGGGAAATAAATAATGT+TGG	0.443669	7.4:-7633502	MS.gene051755:CDS
TATGTTACCTTCGTCATCGC+CGG	0.481783	7.4:-7633650	MS.gene051755:CDS
TTACGGTGTTCACAAGCTCT+GGG	0.588806	7.4:-7633518	MS.gene051755:CDS
CTGGGAAATAAATAATGTTG+GGG	0.603916	7.4:-7633500	MS.gene051755:intron
GGCGCATAAGAACCTTGTAA+AGG	0.605705	7.4:+7633684	None:intergenic
GCTGCTAAAAGAAGAGGAGG+AGG	0.621931	7.4:-7633719	MS.gene051755:CDS
GAATCCGCTGCTAAAAGAAG+AGG	0.626583	7.4:-7633725	MS.gene051755:CDS
AAAAGCACCGGCGATGACGA+AGG	0.627325	7.4:+7633643	None:intergenic
TCCGCTGCTAAAAGAAGAGG+AGG	0.631604	7.4:-7633722	MS.gene051755:CDS
CCGTTCACCGAGAAAAGCAC+CGG	0.644147	7.4:+7633631	None:intergenic
AGGTAACATAGACAGAGTTG+CGG	0.713031	7.4:+7633663	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTAAGAGTAAAATTAAAAG+GGG	+	chr7.4:7633531-7633550	None:intergenic	15.0%
!!	GATTAAGAGTAAAATTAAAA+GGG	+	chr7.4:7633532-7633551	None:intergenic	15.0%
!!	CGATTAAGAGTAAAATTAAA+AGG	+	chr7.4:7633533-7633552	None:intergenic	20.0%
!	TCTGGGAAATAAATAATGTT+GGG	-	chr7.4:7633622-7633641	MS.gene051755:intron	25.0%
	CTGGGAAATAAATAATGTTG+GGG	-	chr7.4:7633623-7633642	MS.gene051755:intron	30.0%
	TCGATAGATTAAGCATAAAG+TGG	+	chr7.4:7633662-7633681	None:intergenic	30.0%
!	CTCTGGGAAATAAATAATGT+TGG	-	chr7.4:7633621-7633640	MS.gene051755:intron	30.0%
	AGGTAACATAGACAGAGTTG+CGG	+	chr7.4:7633463-7633482	None:intergenic	40.0%
	AGATCGCTGAATTTCGTTGC+AGG	-	chr7.4:7633575-7633594	MS.gene051755:intron	45.0%
	ATTACGGTGTTCACAAGCTC+TGG	-	chr7.4:7633604-7633623	MS.gene051755:intron	45.0%
	GAATCCGCTGCTAAAAGAAG+AGG	-	chr7.4:7633398-7633417	MS.gene051755:intron	45.0%
	GGCGCATAAGAACCTTGTAA+AGG	+	chr7.4:7633442-7633461	None:intergenic	45.0%
	TATGTTACCTTCGTCATCGC+CGG	-	chr7.4:7633473-7633492	MS.gene051755:intron	45.0%
	TTACGGTGTTCACAAGCTCT+GGG	-	chr7.4:7633605-7633624	MS.gene051755:intron	45.0%
!	TCCTCCTCTTCTTTTAGCAG+CGG	+	chr7.4:7633405-7633424	None:intergenic	45.0%
!!!	CGTTTTGGAAGGCCTTTACA+AGG	-	chr7.4:7633427-7633446	MS.gene051755:intron	45.0%
	CTGAATTTCGTTGCAGGCTG+TGG	-	chr7.4:7633581-7633600	MS.gene051755:intron	50.0%
	GCTGCTAAAAGAAGAGGAGG+AGG	-	chr7.4:7633404-7633423	MS.gene051755:intron	50.0%
	TCCGCTGCTAAAAGAAGAGG+AGG	-	chr7.4:7633401-7633420	MS.gene051755:intron	50.0%
!	TCGTTGCAGGCTGTGGATTA+CGG	-	chr7.4:7633588-7633607	MS.gene051755:intron	50.0%
!!!	AAGAAGAGGAGGAGGCGTTT+TGG	-	chr7.4:7633412-7633431	MS.gene051755:intron	50.0%
!	CCGGTGCTTTTCTCGGTGAA+CGG	-	chr7.4:7633492-7633511	MS.gene051755:intron	55.0%
!	CCGTTCACCGAGAAAAGCAC+CGG	+	chr7.4:7633495-7633514	None:intergenic	55.0%
!	CGGTGCTTTTCTCGGTGAAC+GGG	-	chr7.4:7633493-7633512	MS.gene051755:intron	55.0%
!	GTCATCGCCGGTGCTTTTCT+CGG	-	chr7.4:7633485-7633504	MS.gene051755:intron	55.0%
!!	AAAAGCACCGGCGATGACGA+AGG	+	chr7.4:7633483-7633502	None:intergenic	55.0%
!!!	AGAGGAGGAGGCGTTTTGGA+AGG	-	chr7.4:7633416-7633435	MS.gene051755:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	7633395	7633750	7633395	ID=MS.gene051755
chr7.4	mRNA	7633395	7633750	7633395	ID=MS.gene051755.t1;Parent=MS.gene051755
chr7.4	exon	7633631	7633750	7633631	ID=MS.gene051755.t1.exon1;Parent=MS.gene051755.t1
chr7.4	CDS	7633631	7633750	7633631	ID=cds.MS.gene051755.t1;Parent=MS.gene051755.t1
chr7.4	exon	7633501	7633548	7633501	ID=MS.gene051755.t1.exon2;Parent=MS.gene051755.t1
chr7.4	CDS	7633501	7633548	7633501	ID=cds.MS.gene051755.t1;Parent=MS.gene051755.t1
chr7.4	exon	7633395	7633400	7633395	ID=MS.gene051755.t1.exon3;Parent=MS.gene051755.t1
chr7.4	CDS	7633395	7633400	7633395	ID=cds.MS.gene051755.t1;Parent=MS.gene051755.t1

Gene Sequence	>MS.gene051755 ATGGAATCCGCTGCTAAAAGAAGAGGAGGAGGCGTTTTGGAAGGCCTTTACAAGGTTCTTATGCGCCGCAACTCTGTCTATGTTACCTTCGTCATCGCCGGTGCTTTTCTCGGTGAACGGGTTAGCTAATTTTCCCCTTTTAATTTTACTCTTAATCGTCTATTCTTATTTGCTTATCGAAGATCGCTGAATTTCGTTGCAGGCTGTGGATTACGGTGTTCACAAGCTCTGGGAAATAAATAATGTTGGGGTACTGAATCTATTCCACTTTATGCTTAATCTATCGAATTTTAAAATTACGCTTCTTATATAACTGCGATTTAATTCTGATCTTCGTGTCGTTTCGCTAGTTTTAA
Protein sequence	>MS.gene051755.t1 MESAAKRRGGGVLEGLYKVLMRRNSVYVTFVIAGAFLGERAVDYGVHKLWEINNVGF