AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051756


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051756.t1 MTR_7g104570 100.000 56 0 0 1 56 1 56 3.46e-35 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051756.t1 AT3G52730 76.786 56 13 0 1 56 1 56 6.33e-27 92.8
MS.gene051756.t1 AT3G52730 76.786 56 13 0 1 56 1 56 6.81e-27 92.4

Find 15 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAGAGGAGGAGGCGTTT+TGG 0.335531 7.4:-7658338 MS.gene051756:CDS
TCTGGGAAATAAATAATGTT+GGG 0.387527 7.4:-7658128 MS.gene051756:CDS
TCGTTGCAGGCTGTGGATTA+CGG 0.406296 7.4:-7658162 MS.gene051756:intron
ATTACGGTGTTCACAAGCTC+TGG 0.424543 7.4:-7658146 MS.gene051756:CDS
CTCTGGGAAATAAATAATGT+TGG 0.443669 7.4:-7658129 MS.gene051756:CDS
TATGTTACCTTCGTCATCGC+CGG 0.481783 7.4:-7658277 MS.gene051756:CDS
TTACGGTGTTCACAAGCTCT+GGG 0.588806 7.4:-7658145 MS.gene051756:CDS
CTGGGAAATAAATAATGTTG+GGG 0.603916 7.4:-7658127 None:intergenic
GGCGCATAAGAACCTTGTAA+AGG 0.605705 7.4:+7658311 None:intergenic
GCTGCTAAAAGAAGAGGAGG+AGG 0.621931 7.4:-7658346 MS.gene051756:CDS
GAATCCGCTGCTAAAAGAAG+AGG 0.626583 7.4:-7658352 MS.gene051756:CDS
AAAAGCACCGGCGATGACGA+AGG 0.627325 7.4:+7658270 None:intergenic
TCCGCTGCTAAAAGAAGAGG+AGG 0.631604 7.4:-7658349 MS.gene051756:CDS
CCGTTCACCGAGAAAAGCAC+CGG 0.644147 7.4:+7658258 None:intergenic
AGGTAACATAGACAGAGTTG+CGG 0.713031 7.4:+7658290 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAGAGTAAAATTAAAAG+GGG + chr7.4:7658264-7658283 None:intergenic 15.0%
!! GATTAAGAGTAAAATTAAAA+GGG + chr7.4:7658265-7658284 None:intergenic 15.0%
!! CGATTAAGAGTAAAATTAAA+AGG + chr7.4:7658266-7658285 None:intergenic 20.0%
! TCTGGGAAATAAATAATGTT+GGG - chr7.4:7658355-7658374 MS.gene051756:CDS 25.0%
! CTCTGGGAAATAAATAATGT+TGG - chr7.4:7658354-7658373 MS.gene051756:CDS 30.0%
AGGTAACATAGACAGAGTTG+CGG + chr7.4:7658196-7658215 None:intergenic 40.0%
AGATCGCTGAATTTCGTTGC+AGG - chr7.4:7658308-7658327 MS.gene051756:CDS 45.0%
ATTACGGTGTTCACAAGCTC+TGG - chr7.4:7658337-7658356 MS.gene051756:CDS 45.0%
GAATCCGCTGCTAAAAGAAG+AGG - chr7.4:7658131-7658150 MS.gene051756:CDS 45.0%
GGCGCATAAGAACCTTGTAA+AGG + chr7.4:7658175-7658194 None:intergenic 45.0%
TATGTTACCTTCGTCATCGC+CGG - chr7.4:7658206-7658225 MS.gene051756:intron 45.0%
TTACGGTGTTCACAAGCTCT+GGG - chr7.4:7658338-7658357 MS.gene051756:CDS 45.0%
! TCCTCCTCTTCTTTTAGCAG+CGG + chr7.4:7658138-7658157 None:intergenic 45.0%
!!! CGTTTTGGAAGGCCTTTACA+AGG - chr7.4:7658160-7658179 MS.gene051756:intron 45.0%
CTGAATTTCGTTGCAGGCTG+TGG - chr7.4:7658314-7658333 MS.gene051756:CDS 50.0%
GCTGCTAAAAGAAGAGGAGG+AGG - chr7.4:7658137-7658156 MS.gene051756:CDS 50.0%
TCCGCTGCTAAAAGAAGAGG+AGG - chr7.4:7658134-7658153 MS.gene051756:CDS 50.0%
! TCGTTGCAGGCTGTGGATTA+CGG - chr7.4:7658321-7658340 MS.gene051756:CDS 50.0%
!!! AAGAAGAGGAGGAGGCGTTT+TGG - chr7.4:7658145-7658164 MS.gene051756:CDS 50.0%
! CCGGTGCTTTTCTCGGTGAA+CGG - chr7.4:7658225-7658244 MS.gene051756:intron 55.0%
! CCGTTCACCGAGAAAAGCAC+CGG + chr7.4:7658228-7658247 None:intergenic 55.0%
! CGGTGCTTTTCTCGGTGAAC+GGG - chr7.4:7658226-7658245 MS.gene051756:intron 55.0%
! GTCATCGCCGGTGCTTTTCT+CGG - chr7.4:7658218-7658237 MS.gene051756:intron 55.0%
!! AAAAGCACCGGCGATGACGA+AGG + chr7.4:7658216-7658235 None:intergenic 55.0%
!!! AGAGGAGGAGGCGTTTTGGA+AGG - chr7.4:7658149-7658168 MS.gene051756:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 7658128 7658377 7658128 ID=MS.gene051756
chr7.4 mRNA 7658128 7658377 7658128 ID=MS.gene051756.t1;Parent=MS.gene051756
chr7.4 exon 7658258 7658377 7658258 ID=MS.gene051756.t1.exon1;Parent=MS.gene051756.t1
chr7.4 CDS 7658258 7658377 7658258 ID=cds.MS.gene051756.t1;Parent=MS.gene051756.t1
chr7.4 exon 7658128 7658175 7658128 ID=MS.gene051756.t1.exon2;Parent=MS.gene051756.t1
chr7.4 CDS 7658128 7658175 7658128 ID=cds.MS.gene051756.t1;Parent=MS.gene051756.t1
Gene Sequence

>MS.gene051756

ATGGAATCCGCTGCTAAAAGAAGAGGAGGAGGCGTTTTGGAAGGCCTTTACAAGGTTCTTATGCGCCGCAACTCTGTCTATGTTACCTTCGTCATCGCCGGTGCTTTTCTCGGTGAACGGGTTAGCTAATTTTCCCCTTTTAATTTTACTCTTAATCGTCTATTCTTATTTGCTTATCGAAGATCGCTGAATTTCGTTGCAGGCTGTGGATTACGGTGTTCACAAGCTCTGGGAAATAAATAATGTTGGG

Protein sequence

>MS.gene051756.t1

MESAAKRRGGGVLEGLYKVLMRRNSVYVTFVIAGAFLGERAVDYGVHKLWEINNVG